Bioactive Carbohydrates and Dietary Fibre
·
2020
·
IF 0
Jinxia Meng,Zhouya Bai,Wenqi Huang,Ying Liu,Peilin Wang,Shaoping Nie,Xiaojun Huang
Type 2 diabetes (T2D), as the most common type of diabetes, has become the public problem in the world for increasing prevalence. In this study, the effects of polysaccharide from white kidney bean (WKBP) on streptozotocin-induced diabetic rats were investigated. Administration of WKBP decreased the levels of blood glucose , glycated serum protein and increased the level of insulin of diabetic rats. Treatment of WKBP reduced the concentration of total cholesterol and raised the ratio of high and low-density lipoprotein cholesterol related to serum lipids . WKBP also can lessen damage of pancreas, kidney and liver, and contribute to their function restoration. The results found that WKBP exhibited high potential on helping with hyperglycemia , hyperlipidemia and alleviating the damage of pancreas, kidney and liver of T2D.
研究领域:
生物化学
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药理学
·
内分泌学
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DOI
Biomolecules
·
2024
·Vol. 14
·期 8
·907
·
IF 4.8
Yusu Wang,Ying Zong,Weijia Chen,Naichao Diao,Quanmin Zhao,Chunyi Li,Boyin Jia,Miao Zhang,Jianming Li,Yan Zhao,Rui Du,Zhongmei He
Due to the limited supply of autologous bone grafts, there is a need to develop more bone matrix materials to repair bone defects. Xenograft bone is expected to be used for clinical treatment due to its exact structural similarity to natural bone and its high biocompatibility. In this study, decellularized antler cancellous bone matrix (DACB) was first prepared, and then the extent of decellularization of DACB was verified by histological staining, which demonstrated that it retained the extracellular matrix (ECM). The bioactivity of DACB was assessed using C3H10T1/2 cells, revealing that DACB enhanced cell proliferation and facilitated cell adhesion and osteogenic differentiation. When evaluated by implanting DACB into nude mice, there were no signs of necrosis or inflammation in the epidermal tissues. The bone repair effect of DACB was verified in vivo using sika deer during the antler growth period as an animal model, and the molecular mechanisms of bone repair were further evaluated by transcriptomic analysis of the regenerated tissues. Our findings suggest that the low immunogenicity of DACB enhances the production of bone extracellular matrix components, leading to effective osseointegration between bone and DACB. This study provides a new reference for solving bone defects.
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产品关联
DOI
PubMed
高影响因子
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2024
·
IF 4.2
Jiying Zhang,Huifang Liu,Jianmei Yao,Chiyu Ma,Wen Yang,Zhiwei Lei,Rongyu Li
Anthracnose, a fungal disease, commonly infects tea plants and severely impacts the yield and quality of tea. One method for controlling anthracnose is the application of citronellol, a plant extract that exhibits broad-spectrum antimicrobial activity. Herein, the physiological and biochemical mechanism by which citronellol controls anthracnose caused by Colletotrichum camelliae was investigated. Citronellol exhibited excellent antifungal activity based on direct and indirect mycelial growth inhibition assays, with EC 50 values of 76.88 mg/L and 29.79 μL/L air, respectively. Citronellol also exhibited good control effects on C. camelliae in semi-isolated leaf experiments. Optical and scanning electron microscopy revealed that citronellol caused C. camelliae mycelia to thin, fracture, fold and deform. Transmission electron microscopy revealed that the mycelial cell walls collapsed inward and separated, and the organelles became blurred after treatment with citronellol. The sensitivity of C. camelliae to calcofluor white staining was significantly enhanced by citronellol, while PI staining showed minimal fluorescence, and the relative conductivity of mycelia were not significantly different. Under citronellol treatment, the expression levels of β -1,3-glucanase, chitin synthase, and chitin deacetylase-related genes were significantly decreased, while the expression levels of chitinase genes were increased, leading to lower chitinase activity and increased β -1,3-glucanase activity. Therefore, citronellol disrupted the cell wall integrity of C. camelliae and inhibited normal mycelial growth.
研究领域:
植物病理学
·
分子生物学
·
真菌学
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产品关联
DOI
PubMed
高影响因子
Oncologie
·
2024
·
IF 1.4
Chenchen Jin,Xuejiao Xu,Tao Li,Chunxue Zhang,Jianqing Peng,Chao Liu,Weifeng Zheng,Xu Zhang
Objectives
Colorectal cancer (CRC) is a global challenge, and heat shock protein 90 (HSP90) is identified as a key driver in cancer progression. However, the tumor-promoting mechanism of HSP90 in CRC, particularly HSP90AB1, remains unclear. This study aims to explore and analyze the oncogenic mechanism of HSP90AB1 in CRC and identify potential therapeutic targets.
Materials and methods
HSP90AB1 expression underwent analysis in CRC cell lines and tissues at mRNA and protein levels. Through the use of shRNA, targeted suppression of HSP90AB1 was achieved in CRC cell lines, enabling analysis of its influence on cell proliferation, invasion, apoptosis, and cell cycle progression. Subsequent investigation focused on elucidating the regulatory relationship between HSP90AB1 and IDO1, employing a combination of bioinformatics approaches and in vitro /vivo experiments. These efforts confirmed IDO1 as a downstream target of HSP90AB1 and provided insight into its role in driving CRC progression.
Results
HSP90AB1 exhibits overexpression in both CRC cell lines and tumor tissues (p<0.05). Its downregulation impedes cell proliferation and invasion (p<0.01), promotes apoptosis and cell cycle arrest (p<0.05). Investigation reveals that decreased HSP90AB1 leads to the inhibition of IDO1 (p<0.01), suggesting that IDO1 regulation plays a crucial role in mediating the pro-tumorigenic effects of HSP90AB1. In vivo experiments confirm the substantial reduction in tumor growth upon HSP90AB1 knockdown in xenograft models (p<0.01). However, this tumor-suppressive effect is reversed upon IDO1 overexpression (p<0.01), highlighting IDO1 as a downstream target of HSP90AB1 in CRC progression.
Conclusions
HSP90AB1 exerts a regulatory role in the progression of CRC by upregulating IDO1.
研究领域:
肿瘤学
·
分子生物学
·
癌症研究
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产品关联
DOI
高影响因子
Nano Today
·
2024
·
IF 13.2
Ziliang Dong,Lei Zhang,Dongxu Zhao,Chunjie Wang,Yu Hao,Quguang Li,Yunyun Zhang,Zhijuan Yang,Caifang Ni,Zhuang Liu,Liangzhu Feng
Transcatheter arterial embolization (TAE) represents a preferred therapeutic modality with minimal invasiveness for unresectable intermediate and advanced hepatocellular carcinoma (HCC) patients. However, its therapeutic outcome is restricted by incomplete occlusion of the whole tumor vasculature and exacerbated tumor immunosuppression due to the lack of ideal embolic agents. Herein, procoagulant embolic microspheres with tunable sizes are prepared by loading thrombin into calcium carbonate (CaCO 3 ) nanoparticle-embedded gelatin microspheres (Th-CaCO 3 @gelatin MSs) via a microfluidic process. In this system, the yielded Th-CaCO 3 @gelatin MSs are capable of occluding main tumor-feeding blood vessels, while the released thrombin and Ca 2+ triggered by the acidic tumor microenvironment can synergistically promote the formation of intravascular blood clots to occlude tumor capillaries. Meanwhile, the embedded CaCO 3 nanoparticles can work as proton sponges to neutralize tumor acidity and thus effectively reverse tumor immunosuppression. Resultantly, TAE treatment with Th-CaCO 3 @gelatin MSs exhibits the most effective tumor suppression efficacy on orthotopic N1S1 HCC rat xenografts without causing obvious toxic effects. Therefore, this study highlights that our biocompatible embolic microspheres are capable of potentiating conventional TAE treatment by promoting pH-responsive tumor vascular infarction and neutralizing the acidic tumor microenvironment, with great potential in clinical translation.
研究领域:
肿瘤学
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医学工程
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生物医学材料
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产品关联
DOI
高影响因子
Nano Energy
·
2016
·
IF 11.553
Qunwei Tang,Hongna Zhang,Benlin He,Peizhi Yang
Future solar cells may produce electricity in all weathers. We present here a cost-effective and high-efficiency graphene based conducting composite tailored all-weather solar cell that can be actuated with raindrops and sunlight. Due to the formation of π-electron|cation double-layer pseudocapacitance at conducting composite/raindrop interface, the delocalized π-electrons on graphene can migrate along percolating pathways forward at spreading process and backward at shrinking periods, resulting in charging and discharging of the pseudocapacitor. The optimized solar cell yields a photoelectric conversion efficiency of 9.8% under simulated sunlight irradiation (air mass 1.5, 100 mW cm −2 ) as well as current over several microamps and voltage of hundreds of microvolts under simulated raindrops. This work could extend our knowledge of future all-weather solar cells.
研究领域:
材料科学
·
能源技术
✓ 已验证
DOI
高影响因子
Food Additives and Contaminants Part A-Chemistry Analysis Control Exposure & Risk Assessment
·
2022
·
IF 3.549
Quancheng Liu,Libo Huang,Zhiwei Cui,Bin Qiao,Fuchang Li,Chunyang Wang
Fumonisin B1 (FB1) has the highest natural contamination rate among all fumonisin analogs and can inhibit food intake and weight gain of pigs. Under laboratory conditions, carboxylesterase FumDSB has a high FB1 degradation rate and excellent pH and thermal stability. The present study sought to estimate the effects of FumDSB on growing pigs from the perspective of a brain–intestinal axis. Twenty-four growing pigs of similar weight were divided into Control, FB1 (5 mg FB1/kg feed), and FumDSB (5 mg FB1/kg and 0.1% FumDSB in the feed) groups. After 42 days of feeding, hypothalamus and jejunum samples were collected for quantitative real-time fluorescence, western blotting, and immunohistochemistry. The results showed that FB1 consumption can destruct the tissue structure of hypothalamus and jejunum, affect the expression and distribution of several appetite-related neuropeptides and inflammatory cytokines, thereby inducing neuroinflammatory responses and affecting food intake and weight gain. However, these anorexia effects and inflammatory responses are alleviated when FumDSB is added to the feed. In short, FumDSB can alleviate the inflammatory response induced by FB1 in growing pigs.
研究领域:
动物科学
·
毒理学
·
营养学
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DOI
PubMed
高影响因子
CHEMICAL PAPERS
·
2019
·Vol. 74
·期 3
·895-901
·
IF 1.246
Gao Ruo-Xuan,Gao Yuan-Yuan,Xie Rui-Jun,Han Li-Min
Ferrocenylcarboxylic acid Fe, Co, and Ni complexes were synthesized as model complexes to investigate how many ferrocene units of multi-ferrocenyl system can react with the electrode. The molecular structures of model complexes were characterized by X-ray single-crystal diffraction, the diffusion coefficient of the complexes and ferrocene was determined by the diffusion-ordered spectroscopy and Einstein–Stokes equation, the electrode reaction numbers of ferrocene units were determined by cyclic voltammetry and the Randles–Sevcik equation. A model of electrode reaction of multi-ferrocenyl system was proposed in this paper, which was used to explore how many ferrocene units of multi-ferrocenyl complexes can react with the electrode.
研究领域:
电化学
·
材料科学
·
分析化学
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产品关联
DOI
高影响因子
ANNALS OF PLASTIC SURGERY
·
2020
·Vol. 84
·期 3
·328
·
IF 1.354
Zhou Jinghe,Zhang Jufang,Wu Lihong,Zhu Fei,Xu Huifang
Background We aimed to study the effect of tetramethylpyrazine/ligustrazine ( TMP ) on the survival rate of adipose-derived stem cell (ADSC) transplantation . Methods Human ADSCs were cultured and subcultured. Detection of cell growth was performed using CCK8 kit. A total of 12 BALB/c nude mice including 4 groups (group 1: ADSC–hyaluronic acid [HA] + phosphate-buffered saline [PBS]; group 2: ADSC-HA + TMP injection; group 3: ADSC-HA + TMP soaking; group 4: ADSC-HA + TMP injection + TMP soaking) were used for the animal experiments. Furthermore, hematoxylin-eosin staining and oil red O staining were conducted for the frozen section from the animal tissues. Besides, RNA was extracted from the samples, and reverse transcriptase–polymerase chain reaction experiment was performed. At last, samples were detected using transmission electron microscope. Results Tetramethylpyrazine/ligustrazine resulted in significant increase for cell proliferation on the seventh day. It was easier to promote the growth of adipose cell after injection and soaking of TMP . Hematoxylin-eosin staining and oil red O stain showed that injection and soaking of TMP could significantly increase the size of fat. The relative expression of peroxisome proliferator–activated receptor γ, CCAAT/enhancer-binding protein α, and Alu in group 4 was significantly higher than that in other groups. Conclusions Tetramethylpyrazine/ligustrazine can improve the survival rate of ADSC transplantation . The possible mechanisms of it may be that TMP induces the expression of transcription factor associated with fat formation including peroxisome proliferator–activated receptor γ, CCAAT/enhancer-binding protein α, and Alu and then promotes the growth of adipose cell.
研究领域:
干细胞研究
·
分子生物学
·
药理学
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产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2017
·
IF 7.504
Jiahao Xia,Feng Li,Shaobo Ji,Huaping Xu
Graphene oxide (GO) is an important two-dimensional material since it is water-soluble and can be functionalized to adapt to different applications. However, the current covalent functionalization methods usually require hash conditions, long duration, and sometimes even multiple steps, while noncovalent functionalization is inevitably unstable, especially under a physiological environment where competing species exist. Diselenide bond is a dynamic covalent bond and can respond to both redox conditions and visible light irradiation in a sensitive manner. Thus, in this work by combining the stimuli response of diselenide bond and the oxidative/radical attackable nature of GO, we achieved the in situ covalent functionalization of GO simply by stirring GO with diselenide-containing molecules in aqueous solution. The covalent functionalization was proved by Fourier transform infrared, time-of-flight secondary ion mass spectrometry, atomic force microscopy, thermogravimetric analysis, and so forth, and the functionalization mechanism was deduced to involve both redox reaction and radical addition reaction according to the X-ray photoelectron spectrscopy, atomic emission spectroscopy, and Raman spectroscopy. Moreover, we modified GO with a biocompatible diselenide-containing polymer (mPEGSe)2 and found selenium-functionalized GO could modulate the balance of reactive oxygen species (ROS). GOSe could decrease ROS level by accelerating the reduction of peroxides when the ROS concentration is high while boosting the ROS level by in situ generating ROS when its concentration is relatively low.
研究领域:
材料科学
·
纳米技术
·
化学
·
生物材料
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产品关联
DOI
PubMed
高影响因子
Food Bioscience
·
2023
·
IF 5.2
Yifei Dong,Na Sun,Qi Ge,Renzhi Lv,Songyi Lin
In this study, SHECN (Ser-His-Glu-Cys-Asn) and SHCMN (Ser-His-Cys-Met-Asn) soybean peptides were used as the research objects to investigate their antioxidant activities and XO (xanthine oxidase) activity reduction effects. The results show that SHECN and SHCMN have strong antioxidant activities, and at the same time protect the oxidative stress damage caused by HUA (hyperuricemia). They can quench XO, change the secondary structure of XO, and reduce its activity, which leads to the reduction of UA (uric acid) levels. Molecular docking demonstrated soybean peptides could bind to XO target proteins, respectively. In this study, SHECN and SHCMN were obtained from soybean peptides. Based on the data, soy peptides may be an attractive natural ingredient for the development of novel functional foods that inhibit XO activity.
研究领域:
食品科学
·
生物化学
·
药理学
✓ 已验证
产品关联
DOI
高影响因子
EXPERIMENTAL CELL RESEARCH
·
2023
·
IF 3.7
Hanqing Zhu,Hongliang Zhang,Jiahui Guo,Chao Zhang,Quanwu Zhang,Fenghou Gao
Mdm2 promotes the ubiquitination and degradation of p53, while Mdm2-p60 can bind to p53 and reduce the Mdm2-induced p53 ubiquitination to improve its stability. USP2a can deubiquitinate and stabilize Mdm2, whether USP2a can regulate Mdm2-p60 needs to be further confirmed and elucidated. We found that oxidative stress can up-regulate USP2a at the post-transcriptional level and induce USP2a to be oxidized by forming inter-subunit disulfide bonds . The oxidized USP2a is closely related with cell apoptosis. In apoptotic cells, oxidized USP2a has enhanced protein stability and further stabilizes Mdm2-p60 through deubiquitination , and the USP2a-Mdm2-p60-p53 axis plays a role in cell apoptosis. Altogether USP2a is oxygen sensitive, oxidized USP2a exerts apoptotic effects through the Mdm2-p60-p53 axis, which provides an experimental basis for regulating p53 apoptotic signaling by targeting USP2a.
研究领域:
细胞生物学
·
分子生物学
·
癌症研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）