BIOREMEDIATION JOURNAL
·
2022
·
IF 2.14
Zhuo-yi Zhou,Yi-cheng Wu,Yi Kuang,Guan-zhu Lin,Hai-yan Fu,Ze-jie Wang
Ibuprofen, a most widely used anti-inflammatory drug, is frequently detectable in surface water, which might negatively affect the ecosystem. The present research investigated acute toxicity of ibuprofen to Chlorella vulgaris, and ibuprofen removal by C. vulgaris was also evaluated via a first order kinetic model. The results showed that ibuprofen in water has certain freshwater ecological risks, and ibuprofen-contaminated water can be effectively treated by C. vulgaris. The growth and the photosynthetic activity of C. vulgaris were decreased with the increase in ibuprofen exposure concentration, while superoxide dismutase and catalase activities were markedly promoted. The dry weight of C. vulgaris exposed to 100 mg/L ibuprofen was determined as 0.238 ± 0.008 g/L, which was 60.82% of the control group (without added ibuprofen in medium). The capacity of C. vulgaris to ibuprofen removal was decreased with the increase in ibuprofen concentration. The removal efficiency reached to 67.25% after 10-day treatment at ibuprofen concentration of 25 mg/L, while that was just 32.77% when the ibuprofen concentration increased to 100 mg/L. The ibuprofen removal by C. vulgaris accorded with the first-order kinetic model, and the removal rate constants of C. vulgaris to different ibuprofen concentrations (25, 50, 75 and 100 mg/L) were determined as 0.115, 0.097, 0.093 and 0.043 d−1, respectively.
研究领域:
环境科学
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水生毒理学
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生物修复
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DOI
高影响因子
JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
·
2021
·Vol. 21
·期 5
·3065-3071
·
IF 0
Pan Sihao,Huang Guanhua,Ding Hui,Wang Ke,Wang Hong
Towards addressing water pollution issues, the development of multifunctional chlorella with applications ranging from sensing pollutants to heavy metal and oil removal is described. The use of chlorella cells, which are widely abundant natural structures, leads to simple and low-cost
mass production of effective functional materials. Bioinspired surface modification approaches mediated by polydopamine can endow chlorella with enhanced adsorption capacity for heavy metals, as well as superhydrophobic, fluorescence and magnetic properties according to the desired application.
The resulting chlorella exhibits excellent heavy metal and oil removal ability, while magnetic propulsion and guidance allow directional motion over long distances for implementation in situ removal. Moreover, it is further demonstrated that chlorella can be used as a biosensor to detect metal
ions by taking advantage of the fluorescence properties of carbon dots. Such use of chlorella provides a new way for the large-scale production of functional materials to tackle water pollution.
研究领域:
环境科学
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生物技术
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材料科学
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DOI
Algal Research-Biomass Biofuels and Bioproducts
·
2025
·
IF 4.5
Jirong Long,Wenguang Zhou,Jing Cao,Yuxuan Mao,Peilun Xu,Jin Liu,Yanghong Yao,Chenguang Wang,Yong Liu
Sporopollenin is a chemically robust biopolymer known for its resistance to environmental degradation and diverse functional potential. This study pioneers the successful extraction of sporopollenin from Chlorella vulgaris cultured in coal-fired flue gas using an innovative alkali-acid two-stage protocol. Multiscale characterization revealed that, despite its irregular morphology, microalgal sporopollenin shares high structural similarity with pollen-derived counterparts. Fourier-transform infrared spectroscopy and elemental analysis confirmed similar functional groups and elemental profiles. Liquid chromatography–mass spectrometry and pyrolysis–gas chromatography–mass spectrometry identified fatty acids as the major structural units, while solid-state 13 C SSNMR spectroscopy revealed a hybrid architecture comprising aliphatic, aromatic, and heterocyclic components. These findings provide molecular insight into the evolutionary continuity of sporopollenin and highlight a scalable, green approach for transforming industrial emissions into high-value biomaterials.
研究领域:
藻类学
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生物聚合物科学
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环境生物技术
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工业生物技术
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材料科学
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分析化学
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DOI
高影响因子
JOURNAL OF FOOD BIOCHEMISTRY
·
2024
·Vol. 2024
·期 1
·3828251
·
IF 3.5
Yan Wang,Xiao Kong,Mengdi Du,Yang Qiao,Minjie Ye,Bing Han,Jing Gan
Chlorella , a single-celled green algae rich in proteins, holds promise as a source of bioactive peptides. This research aimed to isolate an antioxidant peptide from Chlorella , examining its stability through digestion. Using Response Surface Methodology (RSM), we identified optimal hydrolysis conditions for Chlorella Protein Hydrolysate (CPH): 55°C, pH 8.75, and an enzyme-to-substrate ratio of 2.15%. These conditions led to CPH with notable radical scavenging activity against 1,1-diphenyl-2-picrylhydrazyl (DPPH) (53.19 ± 0.99%), 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) (76.86 ± 0.28%), hydroxyl radical (53.39 ± 1.74%), and Fe 2+ chelating rates of 22.56 ± 2.97%. Subsequently, peptides under 3 kDa isolated by ultrafication proved particularly stable and antioxidative after in vitro digestion. In addition, further purify to obtain component C1 with DPPH radical scavenging ability of 59.62 ± 1.29% and an Fe 2+ chelating ability of 49.78 ± 1.55%. Subsequent LC-MS/MS identification and virtual screening led to the discovery of a stable and antioxidative peptide, Ser-Gly-His-His-Lys-Pro-Leu (SGHHKPL). The study provides the theoretical basis for the development and utilization of chlorella .
研究领域:
食品科学
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生物化学
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DOI
高影响因子
Algal Research-Biomass Biofuels and Bioproducts
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2026
·
IF 5
Zhilai Lu,Zhe Luo,Min Liao,Xiaomei Xie,Mingdi Fan,Yue Xiao,Yunqiang Zhu
Two plant growth promoting bacteria (PGPB) were isolated. • PGPB could significantly enhance the biomass and protein content of Chlorella. • PGPB could enhancing the nitrogen assimilation and cell division of Chlorella. • PGPB could inhibit the flow of carbon metabolism to fat. The co-cultivation of microalgae with bacteria that stimulate microalgae growth and metabolic changes is a promising strategy for improving microalgae yield and quality. In this study, two plant growth promoting bacteria (PGPB), Bacillus safensis BAI-6 (BAI-6) and Bacillus altitudinis FEN-9 (FEN-9), were isolated from a long-term open cultivation system of Chlorella sp. LZ-29. Both strains were confirmed to produce the growth-promoting phytohormone indole-3-acetic acid (IAA) and vitamin B12. The changes in biomass, protein, fat, polysaccharide, pigment and other active components of Chlorella sp. LZ-29 under co-cultivation with these strains were investigated. Compared with the axenic Chlorella culture, co-cultivation with BAI-6 or FEN-9 alone increased biomass by 46.57% and 58.75%, respectively, while the dual-strain co-culture achieved the highest biomass increase of 66.46%. Protein content increased by 7% with BAI-6, 2.56% with FEN-9, and 13.37% in the dual-strain co-culture. Conversely, fat content decreased by 3.05%, 3.67%, and 3.48%, respectively, while polysaccharide content also declined in all co-culture treatments ( p < 0.05). The synergistic effect of BAI-6 and FEN-9 in dual co-culture resulted in significantly enhanced biomass and protein production ( p < 0.01), likely due to increased secretion of IAA and vitamin B12, which promoted nitrogen assimilation and cell division, while inhibiting carbon flux towards lipid synthesis. These findings demonstrate that the dual-strain co-culture system has significant potential for enhancing both the yield and protein content of Chlorella sp. LZ-29.
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DOI
高影响因子
Algal Research-Biomass Biofuels and Bioproducts
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2025
·
IF 4.5
Anqi Zhao,Xuejing Duan,Yang Guo,Zhuoqi Yu,Beixiao Zhang,Yongkun Lv,Alexei Solovchenko,Jifeng Chen,Jiangliang Xu
Chlorella based single cell protein (SCP) is a powerful candidate of sustainable protein source. The heterotrophic cultivation has been proved to be a more effective and industrially scalable route for the economical production of the Chlorella biomass, compare to traditional autotrophic cultivation. In this study, the heterotrophic cultivation medium and pulsed feeding strategy were optimized for the enhanced production of Chlorella pyrenoidosa CP2 based SCP. The content of total amino acids, biomass production, biomass productivity of C. pyrenoidosa CP2 respectively reached 50.64 %, 132.13 g/L and 18.87 g/L/d at 5-L fermenter scale. Moreover, the nutrient profile showed that the macronutrient contents, as well as the amino acid compositions and the essential fatty acid proportion of the heterotrophic C. pyrenoidosa CP2 biomass were comparable to those of the autotrophic biomass. The results indicated that the heterotrophic cultivation of C. pyrenoidosa CP2 has great potential for industrial production of microalgae based SCP.
研究领域:
微生物学
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生物工程
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工业生物技术
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应用与环境微生物学
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食品科学
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营养学
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DOI
高影响因子
JOURNAL OF ANALYTICAL AND APPLIED PYROLYSIS
·
2026
·
IF 6.7
Wei Cai,Shengpeng Xia,Liang Zhu,Yixiao Shi,Zhongqing Ma
Microalgae are promising renewable feedstocks for thermochemical conversion due to their rapid growth, high biochemical diversity, and non-competition with food resources. However, the synergistic interactions among carbohydrates, proteins, and lipids during microalgae pyrolysis remain insufficiently understood, hindering the rational control of product distribution. In this study, an improved extraction protocol was developed to obtain high-purity carbohydrate, protein, and lipid fractions from Chlorella vulgaris , enabling systematic evaluation of component-specific and interactive effects during pyrolysis. Co-pyrolysis experiments were conducted using both the extracted fractions and representative model compounds (glucose, aspartic acid, and glycerol monostearate), with product evolution analyzed by Py-GC/MS. The extracted components exhibited thermal decomposition behaviors comparable to those of their corresponding model compounds, confirming the validity of the extraction strategy and model selection. Carbohydrate-protein co-pyrolysis promoted Maillard reactions between amino and carbonyl groups, significantly enhancing the formation of nitrogen-containing compounds such as pyridines and pyrroles. In contrast, protein–lipid interactions favored carbonization pathways, increasing biochar yields while suppressing bio-oil formation. Carbohydrate–lipid co-pyrolysis displayed limited synergy, primarily due to overlapping decomposition pathways and chemically similar oxygenated products. Overall, this work elucidates component-dependent synergistic mechanisms in microalgae pyrolysis and provides a validated framework for predicting and regulating product distributions from microalgal biomass.
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DOI
高影响因子
BIORESOURCE TECHNOLOGY
·
2025
·
IF 9
Jiamin Xu,Shuyue Wang,Peng Peng,Shujuan Zhang
Microalgae-based system represents a promising and eco-friendly solution for mitigating antibiotic pollution. However, their efficacy is often compromised by antibiotics stress. Here, we demonstrate that acetylacetone (AA), a natural small molecule with potential to regulate microalgal growth and metabolism, significantly alleviates tetracycline (TC) toxicity in Chlorella vulgaris , enhancing TC removal in aquaculture wastewater from 1 mg L -1 to 0.01 mg L -1 . The AA-treated effluent showed no significant developmental toxicity to wheat and negligible antibiotic resistance gene propagation. Proteomic and metabolomic analyses revealed that AA restores cellular homeostasis in microalgae by counteracting TC-induced oxidative stress and metabolic dysregulation, thereby sustaining removal performance under stressful conditions. This stress-regulation mechanism enables robust microalgae-based antibiotic degradation while preserving aquatic ecological safety, providing a sustainable strategy for antibiotic-containing wastewater treatment.
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DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2025
·
IF 9
Xiaoxiang Cheng,Shun Song,Dao Xiao,Chengsi Hou,Kunyu Chen,Yan Jin,Xinsheng Luo,Congwei Luo,Tao Yang,Daoji Wu
Chlorella blooms in water bodies caused by eutrophication can pose a potential membrane fouling problem for ultrafiltration. In this study, an innovative combination of far-ultraviolet light (Far-UVC) and permanganate (Mn(Ⅶ)) mild pre-oxidation was proposed to mitigate ultrafiltration membrane fouling primarily induced by Chlorella pollutants. With 30 min Far-UVC/Mn(Ⅶ) treatment, the final J/J 0 surged by 0.65 compared with that of raw water, indicating a good improvement in flux, with R r and R ir respectively decreased by 98.8 % and 76.8 %. The results of the model fitting showed that membrane fouling changed from complete clogging to the cake filtration stage, and the clogging of membrane pores was significantly reduced. Meanwhile, the increase in interfacial free energy also made it difficult for pollutants to adhere to the membrane surface. Mechanism analysis revealed that HO· and reactive manganese (RMnS, including Mn(Ⅲ) and Mn(Ⅴ)) produced during combined pretreatment fulfilled a vital role in the removal of algal foulants. In addition, in situ formation of MnO 2 promoted the aggregation of algal cells, further reducing the fouling load. Under the electrostatic action of MnO 2 , the zeta potential of flocs declined from −21.1 mV to −4.7 mV, indicating a decrease in electrostatic repulsion, which could promote Chlorella cell aggregation. Simultaneously, the content of fluorescent components was also significantly reduced by the oxidation of HO· and RMnS. Far-UVC/Mn(Ⅶ) pretreatment maintained the integrity of Chlorella cells without causing significant damage, thereby preventing the release of intracellular organic matter and effectively reducing the accumulation of algal-related contaminants on the membrane surface. This work provides an effective way to alleviate membrane fouling induced by Chlorella -laden water and advances the practice of Far-UVC oxidation processes.
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DOI
高影响因子
Chemical Engineering and Processing-Process Intensification
·
2025
·
IF 3.9
Juxiang Chen,Sitong Lin,Chong Ruan,Lin Liu,Shangye Yang
The proliferation of sulfonamide antibiotics (SAs) in aquatic ecosystems poses a severe threat to environmental integrity and human well-being. This research employs an acclimation strategy using Chlorella pyrenoidosas to enhance the photo-sensitized degradation efficacy of sulfamethoxazole (SMX) and sulfisoxazole (SIZ). The acclimated Chlorella exhibits remarkable degradation capabilities, with improvements of approximately 9.7 times for SMX and 4.2 times for SIZ, respectively, compared to non-acclimated strains. These improvements are linked to increased growth rates, higher chlorophyll levels, enhanced extracellular organic matter (EOM) production, and elevated antioxidant enzyme activities. The enhanced performance is attributed to refined enzymatic and metabolic processes promoting photosynthesis, energy conversion, and the degradation of SAs. Through response surface methodology, this approach is refined to achieve optimal water remediation results, attaining a degradation rate of 78.04 %. The EOM content increases by up to 68.33 %, while growth rates improve by 1.06 to 1.14 times, indicating superior tolerance. A biphasic response to antibiotic concentrations is also observed, where low concentrations facilitate degradation while high concentrations have an inhibitory effect. This characteristic is reflected by significant increases in antioxidant enzyme activities, particularly in the case of glutathione reductase (GR) and catalase (CAT) at concentrations below 1 mg/L.
研究领域:
环境科学
·
微生物学
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DOI
高影响因子
Water
·
2021
·Vol. 13
·期 10
·1355
·
IF 3.103
Zhineng Dai,Xing Luo,Aili Yang,Jinsong Wang,Haiyan Fu,Yicheng Wu
Triclosan has been widely used as addition ingredient in personal care and medical antibacterial products, and the increasing amounts of triclosan discharged in aquatic environments pose a potential risk to aquatic ecological systems. In this study, we investigated the effects of exposure to varying triclosan concentrations on the growth, chlorophyll fluorescence and antioxidant enzyme activity ofChlorella vulgaris. The results showed that low-concentration triclosan (<0.75 mg/L) can stimulate the growth ofChlorella vulgaris, whereas 1.05 mg/L triclosan exhibited significant inhibition. Low-concentration triclosan (<0.75 mg/L) could improve the tolerance and utilization ability ofChlorella vulgarisin relation to strong light. We observed a significant increase in the malondialdehyde content ofChlorella vulgarisexposed to 1.05 mg/L triclosan. The intracellular superoxide dismutase and catalase (CAT) activities ofChlorella vulgarisexposed to triclosan were higher than the control groups, and the increase in this activity was positively correlated with the concentration of triclosan. The results also showed that excessive H2O2may in turn damage the CAT structure and eventually inactivate CAT activity whenChlorella vulgarisis exposed to 1.05 mg/L triclosan. This study provided a theoretical basis which can be used to evaluate the ecological risk of triclosan in the aquatic environment.Keywords:triclosan;Chlorella vulgaris;eco-toxicological effect;chlorophyll fluorescence
研究领域:
环境科学
·
水生生物学
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产品关联
DOI
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2020
·
IF 4.872
Wenfeng Yang,Xinxin Gao,Yixiao Wu,Liang Wan,Lichen Tan,Shaoman Yuan,Huijun Ding,Weihao Zhang
Microplastics and nonylphenol (NP) are considered as emerging pollutant and have attracted wide attention, while their combined toxicity on aquatic organisms is barely researched. Therefore, the combined toxicity influence of NP with three types of microplastics containing polyethylene (PE1000, 13 μm and PE, 150 μm), polyamide (PA1000, 13 μm and PA, 150 μm) polystyrene (PS, 150 μm) on microalgae Chlorella pyrenoidosa was analyzed. Both growth inhibition, chlorophyll fluorescence, superoxide dismutase (SOD), malondialdehyde (MDA), and catalase (CAT) were determined. We found that single microplastics and NP both inhibited algal growth, thereby causing oxidative stress . The order of inhibition effect in single microplastics experiment was PE1000 > PA1000 > PE ≈ PS > PA. The combined toxicity experiment results indicated that the presence of microplastics had positive effect in terms of alleviating NP toxicity to C. pyrenoidosa , and the microplastics adsorption capacity to NP was the dominant contributing factor for this effect. According to the independent action model, the combined toxicity was antagonistic. Because the negative effect of smaller size microplastics on algal growth was aggravated with prolonged exposure time, the optimum effect of microplastics alleviated NP toxicity was PA1000 at 48 h, while this effect was substituted by PA at 96 h during combined toxicity. Thus, the toxicity of smaller size microplastics has a nonnegligible influence on combined toxicity. This study confirms that microplastics significantly affected the toxicity of organic pollutants on microalgae. Further research on the combined toxicity of smaller size microplastics with pollutants in chronic toxicity is needed.
研究领域:
环境科学
·
毒理学
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产品关联
DOI
PubMed
高影响因子
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