JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2024
·
IF 5.7
Shuli Man,Wei Liu,Jingxian Bi,Jingjing Bai,Qiong Wu,Bin Hu,Jing Hu,Long Ma
Curcumin (CUR) as one of the natural edible pigments is approved by the World Health Organization due to its nontoxic and anticancer effect. However, the utility of CUR is restricted due to its low oral bioavailability. Nanoparticle drug delivery systems like mesoporous silica nanoparticles (MSNs) have been extensively used due to their high specific surface area, high loading rate, and ease of modification. This study developed lactobionic acid (LA)-modified carboxymethyl chitosan (CMCS)-coated MSNs to deliver CUR specifically targeting hepatocellular carcinoma. Among these nanoparticles, LA targets liver cancer cells. CMCS utilizes pH-responsive release of CUR. The LA-CMCS-MSN@CUR (MSN@CUR) were evaluated using several methods, including Fourier transform infrared spectroscopy, transmission electron microscopy, and zeta potential measurements. Liver cellular uptake of MSN@CUR depends on a specific LA receptor-mediated endocytosis mechanism. Additionally, MSN@CUR performed with a better antitumor effect than Cur in H22 orthotopic transplantation of liver cancer and H22 solid tumor mouse models. Treatment with MSN@CUR significantly reduced the protein of VEGF, p-PI3K, and AKT, increased the protein of caspases 3 and 8, ultimately inhibited tumor migration, and promoted apoptosis. This study provides a new path for delivery of natural active ingredients with excellent bioavailability in the antitumor field.
研究领域:
药剂学
·
肿瘤学
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产品关联
DOI
PubMed
高影响因子
Pharmaceutics
·
2019
·Vol. 11
·期 9
·429
·
IF 4.773
Xiaotian Xie,Haijun Wang,Gareth R. Williams,Yanbo Yang,Yongli Zheng,Junzi Wu,Li-Min Zhu
In this study, curcumin-loaded porous poly(lactic-co-glycolic acid) (PLGA) nanoparticles (NPs) were prepared and surface modified with red blood cell membranes (RBCM) to yield biomimetic[email protected]NPs. The NPs displayed a visible cell-membrane structure at their exterior and had a uniform size of 162 ± 3 nm. In vitro studies showed that drug release from non-porous PLGA NPs was slow and that much of the drug remained trapped in the NPs. In contrast, release was accelerated from the porous PLGA NPs, and after the RBCM coating, a sustained release over 48 h was obtained. Confocal microscopy and flow cytometry results revealed that the RBCM-p-PLGA NPs led to a greater cellular uptake by H22 hepatocarcinoma cells than the uncoated analogue NPs, but could avoid phagocytosis by macrophages. The drug-free formulations were highly biocompatible, while the drug-loaded systems were effective in killing cancer cells.[email protected]NPs possess potent anti-tumor activity in a murine H22 xenograft cancer model (in terms of reduced tumor volume and mass, as well as inducing apoptosis of tumor cells), and have no observable systemic toxicity. Overall, our study demonstrates that the use of the RBCM to cloak nanoscale drug delivery systems holds great promise for targeted cancer treatment, and can ameliorate the severe side effects currently associated with chemotherapy.Keywords:erythrocyte membrane;porous PLGA nanoparticle;curcumin;anticancer therapeutics
研究领域:
药学
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纳米技术
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肿瘤学
✓ 已验证
DOI
PubMed
高影响因子
ACS Food Science & Technology
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2026
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IF 3
Qipeixuan Tian,Yiqi Yang,Yuting Wang,Huanhuan Su,David Everett,Wenle He,Qinzheng Yang,Siru Chen,Yun Zhai,Yuying Fu,Teng Li
Developing an efficient curcumin delivery system based on natural food carriers is a hot topic in both fundamental research and industrial applications. This study successfully prepared curcumin-fortified tofu using tofu as a carrier and an emulsified curcumin encapsulation technology. A systematic investigation was conducted from the perspective of physicochemical and in vitro digestive performance and sensory quality. Results indicate that the soy protein–soybean oil emulsion exhibits a high curcumin encapsulation efficiency and loading capacity. Tofu supplemented with curcumin emulsion developed a unique, dense honeycomb structure with significantly enhanced rheological properties. Simulated digestion experiments demonstrated that curcumin-fortified tofu was digested slowly in the gastric stage, effectively delaying the release of curcumin. The extent of release increased significantly during the intestinal stage, showing the potential for intestinal-targeted delivery. Sensory evaluation confirmed that tofu with 0.10 mg/g of curcumin achieved optimal balance in color, texture, aroma, and taste, demonstrating excellent overall acceptability.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2020
·
IF 4.192
Jieyuan Wu,Wei Chen,Yutong Zhang,Xuanxuan Zhang,Jian-Ming Jin,Shuang-Yan Tang
The biosynthetic efficiency of curcumin, a highly bioactive compound from the plant Curcuma longa, needs to be improved. In this study, we performed host cell and biosynthetic pathway engineering to improve curcumin biosynthesis. Using in vivo-directed evolution, the expression level of curcuminoid synthase (CUS), the rate-limiting enzyme in the curcumin biosynthetic pathway, was significantly improved. Furthermore, as curcumin is a highly hydrophobic compound, two cell membrane engineering strategies were applied to optimize the biosynthetic efficiency. Curcumin storage was increased by overexpression of monoglucosyldiacylglycerol synthase from Acholeplasma laidlawii, which optimized the cell membrane morphology. Furthermore, unsaturated fatty acid supplementation was used to enhance membrane fluidity, which greatly ameliorated the damaging effect of curcumin on the cell membrane. These two strategies enhanced curcumin biosynthesis and demonstrated an additive effect.
研究领域:
生物技术
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代谢工程
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微生物学
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生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
International Journal of Nanomedicine
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2016
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IF 4.32
Shan Jiang,Rongrong Zhu,Xiaolie He,Jiao Wang,Mei Wang,Yechang Qian,Shilong Wang
Curcumin (Cur) is a promising photosensitizer that could be used in photodynamic therapy. However, its poor solubility and hydrolytic instability limit its clinical use. The aim of the present study was to encapsulate Cur into solid lipid nanoparticles (SLNs) in order to improve its therapeutic activity. The Cur-loaded SLNs (Cur-SLNs) were prepared using an emulsification and low-temperature solidification method. The functions of Cur and Cur-SLNs were studied on the non-small cell lung cancer A549 cells for photodynamic therapy. The results revealed that Cur-SLNs induced ~2.27-fold toxicity higher than free Cur at a low concentration of 15 μM under light excitation, stocking more cell cycle at G2/M phase. Cur-SLNs could act as an efficient drug delivery system to increase the intracellular concentration of Cur and its accumulation in mitochondria; meanwhile, the hydrolytic stability of free Cur could be improved. Furthermore, Cur-SLNs exposed to 430 nm light could produce more reactive oxygen species to induce the disruption of mitochondrial membrane potential. Western blot analysis revealed that Cur-SLNs increased the expression of caspase-3, caspase-9 proteins and promoted the ratio of Bax/Bcl-2. Overall, the results from these studies demonstrated that the SLNs could enhance the phototoxic effects of Cur.
研究领域:
药学
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纳米技术
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肿瘤学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
METABOLIC BRAIN DISEASE
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2017
·Vol. 32
·期 3
·789-798
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IF 2.297
Wu Xiang,Chen Huixin,Huang Chunhui,Gu Xinmei,Wang Jialing,Xu Dilin,Yu Xin,Shuai Chu,Chen Liping,Li Shun,Xu Yiguo,Gao Tao,Ye Mingrui,Su Wei,Liu Haixiong,Zhang Jinrong,Wang Chuang,Chen Junping,Wang Qinwen,Cui Wei
Post-operative cognitive dysfunction (POCD) is associated with elderly patients undergoing surgery. However, pharmacological treatments for POCD are limited. In this study, we found that curcumin, an active compound derived from Curcuma longa , ameliorated the cognitive dysfunction following abdominal surgery in aged mice. Further, curcumin prevented surgery-induced anti-oxidant enzyme activity. Curcumin also increased brain-derived neurotrophic factor (BDNF)-positive area and expression of pAkt in the brain, suggesting that curcumin activated BDNF signaling in aged mice. Furthermore, curcumin neutralized cholinergic dysfunction involving choline acetyltransferase expression induced by surgery. These results strongly suggested that curcumin prevented cognitive impairments via multiple targets, possibly by increasing the activity of anti-oxidant enzymes, activation of BDNF signaling, and neutralization of cholinergic dysfunction, concurrently. Based on these novel findings, curcumin might be a potential agent in POCD prophylaxis and treatment.
研究领域:
神经科学
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药理学
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老年学
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产品关联
DOI
PubMed
高影响因子
Metals
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2015
·Vol. 5
·期 4
·2401-2412
·
IF 0.883
Yongli Li,Chunxia Zhu,Jinqing Kan
Superparamagnetic nanomaterials are showing great prospects in medical treatments with targeting medicines. A new conductive superparamagnetic nanocomposite, γ-Fe2O3/polyaniline-curcumin (γ-Fe2O3/PANI-curcumin), was prepared by using the interaction between an amino group in polyaniline and a ketone group in curcumin. The γ-Fe2O3/PANI-curcumin nanocomposite showed superparamagnetism (30 emu·g−1) and electrochemical activity, based on the results of magnetization curve and cyclic voltammetry (CV). Transmission electron microscope (TEM) indicated that the particle size of γ-Fe2O3/PANI-curcumin was between 10 and 50 nm. Fourier transform infrared spectra (FT-IR) and X-ray diffraction (XRD) were used to characterize the γ-Fe2O3/PANI-curcumin nanocomposite, confirming that curcumin was immobilized into the γ-Fe2O3/PANI chain. This study provided an academic foundation for developing a new material for immobilizing an anticancer drug.Keywords:curcumin;maghemite;nanocomposites;polyaniline;superparamagnetism
研究领域:
材料科学
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医学
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药学
✓ 已验证
产品关联
DOI
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
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2015
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IF 4.152
Man Li,Min Gao,Yunlan Fu,Chao Chen,Xuan Meng,Aiping Fan,Deling Kong,Zheng Wang,Yanjun Zhao
On-demand curcumin delivery via stimuli-responsive micellar nanocarriers holds promise for addressing its solubility and stability problem. Polymer-curcumin prodrug conjugate micelle is one of such nanosystems. The diversity of linker and conjugation chemistry enabled the generation and optimization of different curcumin micelles with tunable stimuli-responsiveness and delivery efficiency. The aim of the current work was to generate and assess acetal-linked polymeric micelles to enrich the pH-responsive curcumin delivery platforms. Curcumin was slightly modified prior to conjugating to amphiphilic methoxy poly(ethylene glycol)-poly(lactic acid) (mPEG-PLA) copolymer via an acetal bond, whereas an ester bond-linked conjugate was used as the control. The acetal-containing micelles showed a hydrodynamic diameter of 91.1 ± 2.9 (nm) and the accompanying core size of 63.5 ± 7.1 (nm) with a zeta potential of −10.9 ± 0.7 (mV). Both control and pH-labile micelles displayed similar critical micelle concentration at 1.6 μM. The acetal-containing nanocarriers exhibited a pH-dependent drug release behavior, which was faster at lower pH values. The cytotoxicity study in HepG2 cells revealed a significantly lower IC 50 at 51.7 ± 9.0 (μM) for acetal-linked micelles in contrast to the control at 103.0 ± 17.8 (μM), but the polymer residue showed no cytotoxicity upon drug release. The acetal-linked micellar nanocarrier could be a useful addition to the spectrum of currently available stimuli-responsive curcumin nano-formulations.
研究领域:
药学
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纳米技术
·
药物递送系统
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
·
2023
·Vol. 58
·期 5
·2369-2379
·
IF 3.3
Jiayi Bian,Hong Cai,Wenqing Hu,Zhuolin Liu,Zhikun Zhang,Huangxin Sun,Dan Li,Weizhong Zhu,Li Li
Summary The cost-effective strategy of using paper-based materials as alternatives to plastics can aid in reducing environmental stress. Herein, a novel green low-carbon paper-based food-packaging composite was synthesised: curcumin microcapsule-polyvinyl alcohol/beeswax/chitosan (CURM-PVA/BW/CS) composite papers. Scanning electron microscopy and X-ray diffraction results confirmed that curcumin was successfully encased and adhered to the paper. The water vapour permeability and oxygen transmission rate of the composite papers were 42.28 g (m 2 ·d) −1 and 3.41 cm 3 (m 2 ·d·atm) −1 , respectively. The antioxidant capacity of paper was 93.53%, and the mechanical properties were effectively improved by adding natural antioxidants to the coating. Finally, cakes were packaged in the biodegradable 1%CURM-PVA/BW/CS papers exhibited optimal preservation during the storage period, based on the characterisation of the cake peroxide value, acid value and microbiological count. Overall, the CURM-PVA/BW/CS paper showed great potential for food packaging.
研究领域:
材料科学
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食品科学
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环境科学
·
包装工程
✓ 已验证
DOI
高影响因子
Journal of Biomedical Nanotechnology
·
2015
·Vol. 11
·期 3
·403-415
·
IF 5.338
Wang Jiao,Zhu Rongrong,Sun Dongmei,Sun Xiaoyu,Geng Zhengsong,Liu Hui,Wang Shi-Long
Curcumin (Cur) is a naturally derived, novel anti-inflammatory agent, but its poor solubility limits its clinical use. The aim of the present study was to encapsulate Cur into solid lipid nanoparticles (SLNs) to improve its anti-inflammatory activity. The Cur-loaded SLNs (Cur-SLNs)
were prepared using emulsification and low-temperature solidification methods. In contrast to free Cur, the particles were well dispersed in aqueous medium, showing a narrow size distribution with a range of 55 ± 1.2 nm, a zeta potential value of –26.2 ± 1.3 mV, and a high
drug loading efficiency of 37% ± 2.5%. The sustained release of Cur was observed for up to 6 days. The particles displayed enhanced stability in phosphate-buffered saline by protecting the encapsulated Cur against hydrolysis and biotransformation, as well as increasing biocompatibility.
Cur-SLNs were more effective than free Cur at reducing the expression levels of several proinflammatory mediators, including inflammatory cytokines (IL-6, TNF-α, and IL-1β) and nitric oxide (NO), under in vitro conditions. By Western blotting, we found that
Cur-SLNs were more active than free Cur in inhibiting the LPS-induced activation of the inflammatory transcription factor NF-κB through the suppression of IκB kinase activation. Compared to free Cur, Cur-SLNs had an increased intracellular uptake over time (observed
after 24 h) in RAW264.7 cells. Moreover, the Cur-SLNs (≥20 μM) significantly improved RAW264.7 cell viability by inhibiting apoptosis. Thus, these results demonstrated that SLNs could be used as potential anti-inflammatory drug carriers for the treatment of various chronic diseases.
研究领域:
药理学
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纳米技术
·
免疫学
·
药物开发
✓ 已验证
DOI
PubMed
高影响因子
RSC Advances
·
2020
·Vol. 10
·期 41
·24624-24630
·
IF 3.119
Gaizhen Kuang,Qingfei Zhang,Shasha He,Ying Liu
Curcumin (Cur) can be used as a photosensitizer in the photodynamic therapy (PDT) of cancer, but its low bioavailability limits further clinical application. A mesoporous silica-based drug delivery system (PEGylated mesoporous silica nanoparticles, MSN-PEG@Cur) was designed to solve the problem. The successful preparation of MSN-PEG@Cur was characterized by several physico-chemistry techniques. The endocytosis, ROS generation and in vitro anti-cancer efficacy of MSN-PEG@Cur were evaluated in detail step by step. The results indicated that MSN-PEG@Cur could be effectively endocytosed into cells and release Cur, which can promptly generate ROS upon irradiation, achieving effective PDT in cancer treatment. This MSNs-based drug delivery system provides an alternative strategy for Cur loading and PDT of cancer.
研究领域:
药学
·
纳米医学
·
肿瘤学
✓ 已验证
DOI
高影响因子
FOOD HYDROCOLLOIDS
·
2016
·
IF 3.858
Hualin Wang,Lilan Hao,Peng Wang,Minmin Chen,Suwei Jiang,Shaotong Jiang
The aim of the present study was to evaluate the release kinetics and antibacterial activities of curcumin (CUR) loaded in zein (zein-CUR) electrospun fibers towards Escherichia coli ( E. coli , 8099) and Staphylococcus aureus ( S. aureus , ATCC 6538). CUR was successfully loaded in zein fibers by electrospinning technique, and the encapsulation efficiency was close to 100% and the encapsulated CUR still retained its antioxidant capacity. The morphology and size of fibers strongly depended on CUR contents. The interaction between CUR and zein matrix was confirmed to be hydrogen bonding, and the addition of CUR caused a slight increase in the T g of zein matrix. The electrode process of CUR electrooxidation was diffusion-controlled and could be preceded by chemical reaction. The predominant release of CUR from fibers was Fickian diffusion, and First-order model and Hixson-Crowell model could well describe this diffusion behavior. The zein-CUR fibers showed good antibacterial activity towards S. aureus and E. coli , and the inhibition efficiency increased with the increase of CUR contents. However, the antibacterial activity towards S. aureus was better than that towards E. coli . The study displayed that the zein-CUR fibers might have potential as a promising material for antimicrobial applications to inhibit bacterial growth and propagation in active food packaging.
研究领域:
食品科学
·
材料科学
·
微生物学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）