Arabian Journal of Chemistry
·
2021
·
IF 5.165
Aka Alla Martin,Essy Kouadio Fodjo,Zran Vanh Eric-Simon,Zhen Gu,Guangxin Yang,Trokourey Albert,Cong Kong,Hui-Feng Wang
A highly sensitive modified gold (Au) electrode using cysteine-coated silver nanoparticles (Cys-AgNPs) is designed. The electrochemical activity of this designed sensor has exhibited an excellent response for the detection of 3-chloropropane-1,2-diol (3-MCPD). Indeed, the investigation of electrochemical behavior of 3-MCPD at the Cys-AgNPs/Au electrode has shown that the electrocatalytic activity for the 3-MCPD oxidation in NaOH solution is enhanced by three-fold with the presence of Cys-AgNPs film. This Cys-AgNPs film is found to be stable on the Au electrode and the developed electrode can be reused several times while obtaining excellent results. Furthermore, using differential pulse voltammetry technique, the oxidation current peak shows a linear relationship with 3-MCPD concentration between 2.5 ng/mL and 200 ng/mL. The calculated limit of detection (LOD) and limit of quantification (LOQ) are found to be 2.4 ng/mL and 8.6 ng/mL, respectively. A recovery rate ranging from 96% to 105% in the presence of interfering compounds with similar structure to 3-MCPD or not are observed for the determination of 3-MCPD. Moreover, the developed sensor is successfully applied to the determination of 3-MCPD in smoked mackerel and palm oil samples with results which show 3-MCPD concentration ranging from 174 to 439 μg/kg and 895 to 1280 μg/kg in smoked mackerel and palm oil samples, respectively. In addition to the reusability and stability of the electrode, these results suggest that this Cys-AgNPs/Au electrode can be used as an effective sensor to monitor and determine trace level of 3-MCPD in foodstuffs.
研究领域:
分析化学
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食品安全
·
电化学传感器
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2018
·
IF 6.042
Kangming Xiong,Fangjun Huo,Jianbin Chao,Yongbin Zhang,Caixia Yin
Although some progress has been made in distinguishing the detection of biothiols, NIR biothiol fluorescent probes for simultaneously distinguishing detection of Cys, Hcy, and GSH in vivo have not been reported. The design of these probes involves the introduction of NIR fluorophores and multiple binding sites; thus, the integrated design of probes remains a challenge. Although Cys, Hcy, and GSH have common functional groups, a sulfydryl group and an amino group, due to their differences in spatial structure, they may react with multiple binding site probes to produce different reaction products in different bonding mechanisms, resulting in different colors and fluorescent signal changes of the system. Therefore, multiple binding site fluorescent probes can realize their discrimination detection. For an NIR fluorescent probe, it is easier to realize in vivo imaging to promote the research of biothiols in clinical diagnosis. In our work, not only were multiple binding sites constructed in the compound but also NIR fluorophores were introduced. This enables the probe to not only efficiently distinguish detection of Cys/Hcy and GSH but also achieve fluorescence imaging in vivo. We believe this result is a milestone in the discrimination detection of biothiols.
研究领域:
生物化学
·
医学诊断
·
分子探针研究
✓ 已验证
DOI
PubMed
高影响因子
Advanced Science
·
2025
·
IF 14.1
Ting Yu,Yang Li,Jing Li,Yabing Gan,Zhengze Long,Yun Deng,Youyu Zhang,Haitao Li,Peng Yin,Shouzhuo Yao
Adenosine triphosphate (ATP) is a critical intracellular energy currency that plays a key role in various cellular processes and is closely associated with numerous diseases. Similarly, biothiols such as glutathione (GSH), cysteine (Cys), and homocysteine (Hcy) are integral to many physiological and pathological processes due to their strong redox properties. Simultaneous discrimination and detection of ATP and biothiols offer valuable insights into the pathogenesis of conditions such as epilepsy and liver injury. This study introduces the first fluorescent probe, BCR , designed for multifunctional detection of ATP, GSH, Hcy, and Cys. With outstanding optical properties, excellent biocompatibility, high selectivity, and superior sensitivity, probe BCR enables effective imaging of ATP and biothiol dynamics in vivo. Moreover, probe BCR successfully visualizes changes in ATP, GSH, Hcy, and Cys levels in a PTZ-induced epileptic zebrafish model and an APAP-induced mouse liver injury tissue section model. These findings underscore the significant potential of probe BCR for early disease diagnosis and therapeutic applications.
研究领域:
生物化学
·
医学诊断
·
药理学
✓ 已验证
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Yang Zhou,Yuxin Wei,Runqiu Wang,Yanli Wei,Li Wang
A fluorescent sensing method is presented for the determination of L -Cysteine ( L -Cys) based on sulfur quantum dots (SQDs)/MnO 2 nanosheets (MnO 2 NS) nano system. SQDs was prepared from hydrothermal reaction from sublimed sulfur with β-Cyclodextrin as the surface ligand. SQDs have intense fluorescence which will be quenched in the presence of MnO 2 NS. After the addition of L -Cys, the redox reaction of MnO 2 NS and L -Cys results in the recovery of SQDs fluorescence. Under optimal conditions, the sensing system could achieve a sensitive detection of L -Cys with a wide linearity in the range of 8.0 μM-1.0 mM and a detection of limit as 2.44 μM (3σ/k). This sensing method also shows an excellent specificity and anti-inference, which has a great potential application for the real sample detection. The SQDs-MnO 2 NS served as a promising sensing system for the identification of L -Cys.
研究领域:
分析化学
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纳米技术
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生物传感
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
TOXICOLOGY LETTERS
·
2024
·
IF 2.9
Yingxin Pang,Xiaoqi Tao,Zongmin Qin,Muran Jiang,Erqun Song,Yang Song
This work investigated the influence of surface chirality on cellular internalization, cytotoxicity, and tissue distribution of silver nanoparticles (AgNPs). D-cysteine and L-cysteine are chiral forms of the amino acid cysteine. These enantiomers exhibit distinct spatial arrangements, with D-cysteine having a different configuration from L-cysteine. This structural dissimilarity can lead to variations in how these forms interact with biological systems, potentially impacting their cytotoxic responses. Four distinct types of AgNPs were synthesized, each possessing a unique surface coating: pristine AgNPs (pAgNPs), L-cysteine coated AgNPs (AgNPs@L-Cys), D-cysteine coated AgNPs (AgNPs@D-Cys), and racemic AgNPs coated with both L-Cys and D-Cys (AgNPs@L/D-Cys). We found chiral-dependent cytotoxicity of AgNPs on J774A.1 cells. Specifically, AgNPs@L-Cys exhibited the highest toxicity, and AgNPs@D-Cys exhibited the lowest toxicity. Meanwhile, the cellular uptake of the AgNPs correlated nicely with their cytotoxicity, with AgNPs@L-Cys being internalized to the greatest extent while AgNPs@D-Cys displays the least internalization. Scavenger receptors and clathrin predominantly mediate the cellular internalization of these AgNPs. Strikingly, the dissimilar cellular internalization and cytotoxicity of AgNPs with different chirality were eliminated upon protein corona coverage. Notably, following intravenous injection in mice, these four types of AgNPs showed similar patterns among various organs due to the inevitable protein adsorption in the bloodstream. These findings underscored the pivotal role of surface chirality in governing the biological interactions and toxicity of AgNPs.
研究领域:
纳米技术
·
毒理学
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生物材料科学
·
细胞生物学
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产品关联
DOI
PubMed
高影响因子
APPLIED SURFACE SCIENCE
·
2024
·
IF 6.3
Mengtian Cui,He Wang,Xia Fan,Jianhao Zhang,Changrui Xing,Wenjing Yan
The widespread use of organophosphorus pesticides (OPPs) in foods, poses a serious threat to human and environmental health, it is crucial to develop methods that can efficiently degrade residual OPPs . Herein, a chiral TiO 2 photocatalyst was developed by modification TiO 2 with D-cys/Au NPs (DAT) for the simultaneous degradation of four OPPs (chlorpyrifos, chlorpyrifos-methyl, malathion, and parathion) by natural light. The influence of D-Au NPs concentration on the degradation process was optimized, along with the initial pesticide concentration. Under optimal conditions, the photocatalytic degradation efficiencies of DAT for four OPPs were 96.7 %, 56.9 %, 42.6 %, and 49.2 %, respectively, after 12 h of natural light exposure. 12 degradation products of OPPs were identified, among which three products were identified for the first time. The degradation pathway revealed that the degradation of OPPs by DAT involves the oxidation of the P═S bond and the cleavage of the P-O bond and C-O bond. This study establishes a theoretical foundation for applying chiral TiO 2 nanomaterials for the photocatalytic degradation of OPPs in foods.
研究领域:
环境科学
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食品科学
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材料科学
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产品关联
DOI
高影响因子
MOLECULES
·
2024
·Vol. 29
·期 24
·6011
·
IF 4.2
Ting Zhou,Luyao Zang,Xia Zhang,Xia Liu,Zijie Qu,Guodong Zhang,Xiufeng Wang,Fang Wang,Zhiqing Zhang
Gold nanoclusters (AuNCs) have been widely investigated because of their unique photoluminescence properties. However, the applications of AuNCs are limited by their poor stability and relatively low fluorescence. In the present work, we developed nanocomposites (L-Cys-AuNCs@ZIF-8) with high fluorescence and stability, which were constructed by encapsulating the water-dispersible L-Cys-AuNCs into a ZIF-8 via Zn2+-triggered growth strategy without high temperature and pressure. The maximum emission wavelength of the L-Cys-AuNCs@ZIF-8 composite was at 868 nm, and the fluorescence intensity of L-Cys-AuNCs@ZIF-8 was nearly nine-fold compared with L-Cys-AuNCs without the ZIF-8 package. The mechanism investigation by fluorescence spectroscopy and X-ray photoelectron spectroscopy showed that L-Cys-AuNCs@ZIF-8 impeded ligand rotation, induced energy dissipation, and diminished the self-quenching effect, attributing to the spatial distribution of L-Cys-AuNCs. Based on the high fluorescence efficiency of L-Cys-AuNCs@ZIF-8, a “signal off” detective platform was proposed with copper ions as a model analyte, achieving a sensitive detection limit of Cu2+at 16.7 nM. The quenching mechanism was confirmed, showing that the structure of the L-Cys-AuNCs@ZIF-8 nanocomposites was collapsed by the addition of Cu2+. Attributing to the strong adsorption ability between copper ions and pyridyl nitrogen, the as-prepared L-Cys-AuNCs@ZIF-8 was shown to accumulate Cu2+, and the Zn2+in ZIF-8 was replaced by Cu2+.
研究领域:
材料科学
·
分析化学
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2023
·Vol. 13
·期 11
·7425-7431
·
IF 3.9
Wenjie Luo,Changxu Wang,Jieshu Min,Huiyu Luo
Gold nanoclusters exhibit attractive properties owing to their excellent biocompatibility and strong photostability in the biomedical domain. In this research, cysteine-protected fluorescent gold nanoclusters (Cys-Au NCs) were synthesized via decomposing Au(I)–thiolate complexes for the detection of Fe3+ and ascorbic acid in a bidirectional “on–off–on” mode. Meanwhile, the detailed characterization confirmed that the mean particle size of the prepared fluorescent probe was 2.43 nm and showed a fluorescence quantum yield of 3.31%. In addition, our results indicate that the fluorescence probe for ferric ions exhibited a broad detection scope ranging from 0.1 to 2000 μM and excellent selectivity. The as-prepared Cys-Au NCs/Fe3+ was demonstrated to be an ultrasensitive and selective nanoprobe for the detection of ascorbic acid. This study indicated that the “on–off–on” fluorescent probes Cys-Au NCs held a promising application for the bidirectional detection of Fe3+ and ascorbic acid. Furthermore, our novel “on–off–on” fluorescent probes provided insight into the rational design of thiolate-protected gold nanoclusters for biochemical analysis of high selectivity and sensitivity.
研究领域:
生物医学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2023
·
IF 4.1
Chao Huang,Chunjing Tang,Ruizhi Tang,Zheng Gao,Shujuan Ma,Bolin Gong,Junjie Ou
A hydrophilic adsorbent (Cys@poly(AMA)@MAR) was successfully prepared for the enrichment of N-glycopeptides via surface-initiated atom transfer radical polymerization (SI-ATRP) and photo-initiated “thiol-ene” reaction using monodisperse macroporous adsorbent resin (MAR) as adsorption matrix. Due to the presence of electron-deficient acrylic groups and electron-rich vinyl groups in allyl methacrylate (AMA), both of them can participate in free radical reaction. Therefore, the polymerization time of SI-ATRP was optimized. The resulting poly(AMA)@MAR was modified with l -cysteine (L-Cys) via photo-initiated “thiol-ene” reaction, and the amount of vinyl retained was determined by measuring the adsorption of Cu 2+ . The Cys@poly(AMA)@MAR pendant brushes with high density of amine and carboxyl groups could capture N-glycopeptides from IgG digest and human serum digest by hydrophilic interaction. The 22 N-glycopeptides were identified from IgG digest and the limit of detection reached 10 fmol. The 319 N-glycosylation sites and 583 N-glycopeptides were identified from 2 μL human serum digest and mapped to 147 glycoproteins. It demonstrates great potential and commercialization prospects for the enrichment of N-glycopeptides.
研究领域:
生物化学
·
分析化学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
COMPOSITES PART A-APPLIED SCIENCE AND MANUFACTURING
·
2025
·
IF 8.9
Xiang Li,Wenjie Zhao,Guosong Wu,Yinghao Wu
The harsh service environment in the splash zone poses serious challenges for the long-term durability of steel pipe piles. The basalt fibers (BF) reinforced-composite coatings have acquired great attention in various fields such as offshore wind power equipment and construction industry due to the high strength and chemical stability. However, the service life of BF reinforced-composite coatings is greatly restricted by poor interfacial compatibility between BFs and polymer resin. In this work, we fabricated a BF reinforced epoxy powder composite coating with excellent erosion resistance by constructing L-Cys@Cu MOF on BFs. The interfacial shear strength value between MBF and EP was 49.66 % larger than that of BF. Contrasted to EP, the adhesion strength between 2.5 %MBF/EP coating and the steel substrate was 79.65 MPa, which was increased by 24.67 %. Interestingly, the mass and volume loss of 2.5 %MBF/EP coating was decreased by 22.77 % and 21.16 %, respectively, compared with 2.5 %CBF/EP coating. These results demonstrate the broader applicability of BF in composite materials, arising from the synergistic effect of mechanical interlocking between MBF and EP and the formation of cross-linking network between L-Cys@Cu MOF and EP. This work provides a novel perspective for the development of BF reinforced-composite coatings with long-term erosion resistance.
研究领域:
材料科学
·
腐蚀工程
·
复合材料
·
表面改性
·
海洋防护涂层
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2020
·
IF 5.339
Fangbin Fan,Licheng Wang,Yijing Li,Xusheng Wang,Xiaofeng Lu,Yong Guo
l -Cysteine (L-Cys) and N-Isopropyl acrylamide (NIPAM)-modified silica spheres as a novel stationary phase for hydrophilic interaction liquid chromatography (HILIC) was produced firstly by cross-linking polymerization. Some characterizations in this article confirmed that the synthesis of Cys-Si-NIPAM is successful. Some polymer layers can be observed through transmission electron microscopy (TEM). In addition, through nitrogen adsorption porosity method, scanning electron microscopy (SEM), thermal gravimetric analysis (TGA) and other characterization methods, we can find the significant changes after modify. It has good hydrophilic property and higher column effect than bare silica column, Si-Cys column and Si-NIPAM column under the same conditions. It has good separation effect for some hydrophilic analytes such as 5 nucleosides and nucleoside bases, 5 amino acids , 4 sulfonamide drugs and 10 saccharides . The effects including column temperature, pH and organic solvent content on chromatographic performance were studied, which proved that hydrophilic interactions can be simultaneously existed between the stationary phase and the analytes. In addition, reproducibility and efficiency of the Cys-Si-NIPAM column was also investigated, the results illustrated that the stationary phase have passable stability (the intraday RSDs 0.08–0.44%, n = 3 and the interday RSD 0.46–3.50%, n = 3) and ideal efficiency (plates per meter, ~45700 plates/m). In conclusion, the preparation process of this hydrophilic liquid chromatography stationary phase is not only simple, but also can meet the basic requirements for the separation of hydrophilic analytes.
研究领域:
分析化学
·
色谱技术
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2025
·
IF 11.3
Yang Li,Jing Li,Ting Yu,Hongyi Cai,Youyu Zhang,Haitao Li,Huijun Zhou,Peng Yin
The Mercury (II) ion (Hg²⁺) is a toxic heavy metal that threatens biological systems by inducing oxidative stress and disrupting the redox balance. Biothiols such as cysteine (Cys), homocysteine (Hcy), and glutathione (GSH) are critical in maintaining redox homeostasis and are implicated in numerous physiological and pathological processes. Understanding the complex interactions between Hg²⁺ and biothiols requires molecular tools capable of simultaneous detection. Herein, we report a novel multi-signal fluorescent probe, DPC , designed with four specific recognition sites and rhodamine and coumarin fluorophores. The DPC probe enables the selective and sensitive differentiation of Mercury (II) ion (Hg²⁺), cysteine (Cys), homocysteine (Hcy), and glutathione (GSH), with distinct fluorescence signals. DPC can detect mercury (II) ions (Hg²⁺) in water samples with a high recovery rate ranging from 90.44 % to 112.27 %. DPC was also successfully applied for real-time imaging of these species in living cells and zebrafish, revealing that Hg²⁺ induces oxidative stress, reducing cysteine (Cys), homocysteine (Hcy), and glutathione (GSH) levels. Furthermore, detoxifying agents such as sodium selenite (Na₂SeO₃), 2,3-dimercaptopropan-1-ol (DMPC), and L-selenocysteine (Sec) restored biothiol levels, counteracting Mercury (II) ion (Hg²⁺) toxicity. These results highlight the exceptional performance of DPC for the simultaneous imaging of Mercury (II) ions (Hg²⁺) and biothiols, providing insights into their interactions and proposing a chemobiological framework for studying heavy metal toxicity and developing detoxification strategies.
研究领域:
生物化学
·
毒理学
·
分析化学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）