BIOMACROMOLECULES
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2026
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IF 5.4
Ibrahim M. Ammar,Al-Hassan S. Mahdy,Saikat Kumar Panja,Kui Tan,Shanmeng Lin,Quan Gao,Jin Geng
The need for multifunctional polymers in cellular environments arises from their potential applications in cancer treatment, drug delivery, gene delivery, imaging, sensing of different biomolecules, environmental and cellular engineering, etc. However, due to certain limitations, the direct polymerization of multifunctional polymers within cells is not feasible, as it faces many challenges. Therefore, this study emphasizes the synthesis of functionalized molecules outside the cells and subsequent modification of the polymers inside the cells through intracellular postpolymerization modification (iPPM). We investigate Förster resonance energy transfer (FRET) as a technique for confirming the occurrence of postpolymerization reactions in cells in real time without the need for extraction or purification. The FRET reaction consists of 7-nitrobenz-2-oxa-1,3-diazole (NBD) as the FRET donor, integrated as a segment in the polymer backbone, and rhodamine B-polyethylene glycol-dibenzocyclooctyne (RhB-PEG-DBCO) as the FRET acceptor. A copper-free click chemistry method is used as a postpolymerization reaction within cells by the reaction between the azide group on the polymer backbone and DBCO in the FRET acceptor. By employing FRET and a targeted approach, this technique contributes to the development of multifunctional polymers for diverse applications in cellular environments.
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WATER RESEARCH
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2026
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IF 12.4
Hao Li,Xingrui Tang,Qiang Xian,Shibing Zuo,Yiyang Zeng,Chen Xu,Jun Wen
Electrochemical method offers a promising route for the continuous uranium extraction from seawater, thereby holding significant potential to ensure a sustainable supply of uranium for the nuclear energy industry. The electrode material plays a critical role in determining uranium extraction efficiency, demanding excellent mass transfer performance and high selectivity for effective extraction. Herein, inspired by the hierarchically ordered, porous, and intercross-linked vascular bundle structure of lotus petiole, we report the rational design and successful synthesis of zwitterionic ordered porous electrodes (SBMA-MPAO). This biomimetic architecture not only enhances charge/ion transfer rate and ensures sufficient seawater transport, but also provides numerous adsorption sites for uranyl capture. Furthermore, the integration of zwitterionic moieties enables a synergistic interaction with uranyl species, significantly enhancing extraction efficiency. In natural seawater, the SBMA-MPAO electrode achieves an outstanding uranium adsorption capacity of 9.54 mg/g within 72 hours, including a remarkably high uptake rate of 4.58 mg/g/d on the first day, while maintaining excellent selectivity throughout the process. This work proposes a novel strategy to construct high-performance electrodes for uranium extraction from seawater.
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SEPARATION AND PURIFICATION TECHNOLOGY
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2026
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IF 9.1
Shuo Gao,Wanru Feng,Junsu Jin,Jianguo Mi,Hong Meng
Structured adsorbents capable of combining strong low-pressure CO 2 affinity, high selectivity, and humidity tolerance are highly desired for low-concentration CO 2 capture, yet remain difficult to realize because shaping often compromises porosity and mass transport. Here, we report a solvent-directed and nanoconfined crystallization strategy to construct millimeter-sized polyacrylate (PA)-supported Zn 2 (Mtz) 2 Ox-EG sphere (Zn 2 (Mtz) 2 Ox-EG@PA). Ethylene glycol modulates the ultramicroporous environment of Zn 2 (Mtz) 2 Ox, enhancing its low-pressure CO 2 affinity, while the confined macroporous network of the PA matrix promotes heterogeneous nucleation and suppresses free crystal growth, sharply reducing the size of the micrometer-scale MOF bulk crystals by nearly 100-fold. The resulting Zn 2 (Mtz) 2 Ox-EG@PA composite exhibits a high CO 2 uptake for 1.68 mmol g −1 at 0.05 bar and 313 K, while N 2 and CH 4 adsorption are strongly suppressed. Consequently, high IAST selectivities of 3447 for CO 2 /N 2 and 1275 for CO 2 /CH 4 are achieved at a 5/95 gas ratio. The hydrophobic MOF framework and PA matrix endow the composite with excellent hydrothermal durability, retaining framework integrity after hot-water treatment at 403 K for 7 days. Dynamic breakthrough experiments further demonstrate efficient CO 2 /N 2 separation from a 5 vol% CO 2 stream at 90% RH, highlighting the potential of nanoconfined MOF crystallization for robust structured adsorbents under humid dilute-CO 2 conditions.
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MACROMOLECULAR RAPID COMMUNICATIONS
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2025
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IF 4.3
Sujan Tiwari,Al-Hassan S. Mahdy,Namrata Sapkota,Ibrahim M. Ammar,Saikat Kumar Panja,Shiling Zhang,Xianzhu Zheng,Qi Xing,Jin Geng
The design of polymeric materials with well-defined, tunable properties is essential for advancing drug delivery systems and other biomedical applications. Optimizing key parameters such as size, surface charge, and temperature responsiveness can enhance targeted drug delivery efficiency and biocompatibility. In this study, we synthesized a series of fluorescently labeled polymers with varying molecular weights and charges via reversible addition-fragmentation chain transfer (RAFT) polymerization to investigate their role in cellular uptake. Comprehensive characterization confirmed that these polymers exhibited precise size and charge distributions, enabling controlled interactions with biological systems. Cellular uptake studies in HeLa cells revealed a strong dependence on polymer size, charge, and temperature, with smaller and positively charged polymers demonstrating superior internalization under physiological conditions (37°C). Further analysis showed that the polymers predominantly localized in endosomes and lysosomes, indicating endocytosis as the primary internalization pathway across different polymer charge types. Overall, these findings provide preliminary insights into how polymer physicochemical properties modulate cellular uptake, which may inform the design of future polymer-based drug delivery systems.
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Polymer Chemistry
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2025
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IF 3.9
Jiayi Zhang,Ruyue Cao,Xiaowei Wang,Yixuan Wang,Anchao Feng
This study introduces a novel approach combining reversible addition–fragmentation chain transfer (RAFT) polymerization and hetero-Diels–Alder (HDA) reactions to efficiently synthesize hyperbranched polymers (HBPs) with controlled topology. The traditional ABn monomer methods for creating HBPs face limitations due to random polymerization and the risk of gelation. By optimizing the ABn system into an ABx macromonomer framework, this new RAFT–HDA method enables controlled polymerization, reducing intramolecular cyclization and topological defects and broadening the range of possible polymer architectures. Additionally, more readily available and widely used novel dienes and dienophiles have been identified for the HDA reaction. The versatility of this approach was demonstrated by synthesizing a variety of HBPs with different branching degrees and molecular weights, which were thoroughly characterized by NMR, FTIR, GPC, and DLS techniques. This study provides a robust and efficient pathway for synthesizing complex polymer structures, demonstrating that the RAFT–HDA strategy enables the production of well-defined hyperbranched polymers with significant potential for applications in nanomaterials, biomedicine, and advanced functional materials.
研究领域:
材料科学
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化学工程
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Journal of Inflammation Research
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2025
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IF 4.1
Siyue Bo,Xiaotong Wang,Jiani Qian,Guoqiang Ma,Zheng Ying,Duanmin Hu,Chunyan Hou,Junfeng Ma,Longjiang Xu,Shuang Yang
Background Ulcerative colitis (UC) disrupts the colon’s protective mucus layer, exposing the epithelium to bacteria and triggering inflammation. This barrier, crucial for intestinal health, depends on complex glycosylation, notably sialic acid modifications. However, the precise role of sialic acid acetylation and the enzyme SIAE (sialic acid acetylesterase) in UC pathogenesis remains unclear. This study investigates the role of glycosylation changes, specifically sialic acid de-acetylation, in UC progression.Methods Tissue samples were obtained from patients with ulcerative colitis (UC) and colorectal cancer at the Second Affiliated Hospital of Soochow University. HT-29 cells were utilized to investigate the molecular mechanisms of SIAE in UC pathogenesis. Mass spectrometry was performed to analyze differences in protein and glycoprotein expression. Western blot (WB) and immunohistochemistry (IHC) were used to examine SIAE protein expression changes during inflammation. Furthermore, polymerase chain reaction (PCR) and immunofluorescence were employed to determine the effects of SIAE on sialic acid levels and mucosal immunity.Results In this study, we characterized proteins and glycoproteins from patient tissues with UC, finding that sialic acid acetylesterase (SIAE) is upregulated in UC. HT-29 cells exposed to TNF-α induced an inflammatory response with a 5-fold increased expression of SIAE and NEU1 when TNF-α was at a concentration of 100 ng/mL. Mass spectrometry analysis revealed a reduction in acetylation on glycans and glycoproteins, while confocal microscopy confirmed a decrease in sialic acid on the cell surface. Gene expression analysis showed that CDH1, CTNND1, and ITGA8 were significantly downregulated in HT-29 cells stimulated by TNF-α, suggesting a reduction in cell-cell adhesion. SNA lectin-confocal microscopy revealed a reduction of sialic acid on HT-29 cells in TNF-α-induced UC cell models.Conclusion This study demonstrates that SIAE is significantly upregulated in ulcerative colitis (UC) tissues and TNF-α-stimulated HT-29 cells, leading to a marked reduction in sialic acid acetylation and cell surface sialic acid levels. These changes correlate with decreased expression of cell adhesion molecules, suggesting a disruption of the mucosal barrier integrity. Consequently, SIAE-mediated sialic acid de-acetylation emerges as a critical factor in UC pathogenesis, potentially serving as both a valuable biomarker and a promising therapeutic target.
研究领域:
医学
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分子生物学
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免疫学
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JOURNAL OF CHROMATOGRAPHY A
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2025
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IF 4
Xinrui Xu,Xiaomeng Wang,Hongchuan Wang,Zhiyong Chen
Green liquid chromatography (LC) has garnered increasing attention; however, its adoption as a replacement for conventional methods is limited by inferior separation performance. Herein, we present a fully integrated design for green LC, encompassing biomass-derived starting materials, the green synthesis of a solid stationary phase, and environmentally benign separation using ethanol as the mobile phase. Via a green precipitation polymerization technique (room temperature quiescent redox-initiated precipitation polymerization, RTQRPP), we synthesized a series of monodisperse microspheres derived from natural fatty acids. Notably, microspheres composed of poly(divinylbenzene-co-tetradecyl methacrylate) (P(DVB-TMA))-where tetradecyl methacrylate (TMA) is derived from myristic acid-exhibited a uniform particle size of 4.6 μm (polydispersity index, PDI < 1.01). The resulting P(DVB-TMA) material displays exceptional superoleophilicity and superhydrophobicity, with water contact angles reaching 160 ° Consequently, columns packed with P(DVB-TMA) generated significantly lower back pressure under pure water compared to commercial C18 silica columns. Furthermore, when using an ethanol mobile phase, the P(DVB-TMA) column successfully achieved baseline separation of a four-component dye mixture, demonstrating markedly better resolution than a commercial C18 column under identical conditions. Molecular dynamics simulations and density functional theory calculations confirmed a strong reversed-phase retention mechanism, with elution order correlating with decreasing aqueous solubility (Log S) at 25 ℃. The column reproducibility is proved by that the relative standard deviations (RSD) of retention times can be controlled in 0.40∼0.70 % for the intra-day and 0.77∼1.84 % for the inter-day tests, respectively. Our findings establish this novel stationary phase as a superior, greener alternative to commercial columns for target compound separation.
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MRS Advances
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2025
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IF 0.9
Wang Xiaodong,Yu Xinrun,Xu Yushan,Yu Juan,Huang Pei
A series of PI aerogel composite films were prepared through adjustments made to the ratio of dianhydride and chemical imidization reagents, the crosslinker content, and the utilization of a non-solvent induced phase separation process. The prepared PI aerogel films exhibited a minimum dielectric constant of 1.39 (10 6 Hz), a maximum water contact angle of 137.8°, and demonstrated excellent recyclability. Furthermore, the prepared polyimide aerogel films demonstrated exceptional thermally stable characteristics, with Td 5% maintained at approximately 510 °C, Tg values remaining above 230 °C, and a low coefficient of thermal expansion, at 55.8 ppm/K. The prepared PI aerogel films exhibit a high degree of porosity (74.1%), a relatively low density (0.392 g/cm 3 ), and considerable tensile strength (up to 6.2 MPa). It has been demonstrated that these polyimide aerogel films are highly promising for the construction of high-frequency microelectronic devices. Graphical abstract
研究领域:
材料科学
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高分子科学
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纳米技术
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电子工程
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化学工程
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JOURNAL OF HAZARDOUS MATERIALS
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2019
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IF 7.65
Ming-Yong Zhou,Peng Zhang,Li-Feng Fang,Bao-Ku Zhu,Jian-Li Wang,Jiang-Hua Chen,Heba Abdallah
The development of highly efficient membranes technology using low-pressure driven filtration process, is one of the principal challenges in the wastewater treatment field, especially those aimed at the removal of trace heavy metals. In this work, a novel positively charged tight ultrafiltration (PCTUF) membrane was developed to remove heavy metal cations (Mn 2+ , Co 2+ , Ni 2+ , Zn 2+ and Cd 2+ ) from contaminated waters via electrostatic repulsion mechanism. The PCTUF membrane was fabricated from a new polymer with poly (vinyl chloride co dimethylaminoethyl methacrylate), P (VC- co -DMA) via a nonsolvent induce phase separation (NIPS) process and following facile surface quaternization. The quaternization conditions, the pore structures and chemical properties of the membranes were investigated in detail. The optimally quaternized membrane possessed a positively charged surface and 3.27 nm charged channel with the water permeability of 84 L m −2 h −1 bar −1 . The rejections of heavy metal cations surpassed 95% for feed solutions containing 10 ppm heavy metal. Moreover, the influences of feed concentrations and the operating condition with pressure and pH on the membrane performances were also investigated. The results revealed that the prepared PCTUF membrane with its high perm-selectivity performance provides a worthy reference for highly efficient removal of heavy metal cations.
研究领域:
环境科学
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材料科学
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废水处理技术
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ACS Applied Materials & Interfaces
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2016
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IF 7.145
Zhao Dang,Libin Liu,Yan Li,Yu Xiang,Gailan Guo
The development of stimuli-responsive materials with the ability of controllable oil/water separation is crucial for practical applications. Here, a novel pH responsive nonfluorine-containing copolymer was designed. The copolymer together with silica can be dip-coated on different materials including cotton fabric, filter paper, and polyurethane foam. The coated materials exhibit switchable superhydrophilicity and superhydrophobicity and can be applied in continuous separation of oil/water/oil three phase mixtures, different surfactant stabilized emulsion (oil-in-water, water-in-oil, and oil-in-acidic water) as well as oil uptake and release via in situ and ex situ pH change. We expect that the coatings highlight the practical applications because of the cost-effective preparation process and fluorine-free strategy.
研究领域:
材料科学
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环境工程
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CARBOHYDRATE POLYMERS
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2019
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IF 6.044
WenBo Du,Amin Deng,Juan Guo,Jian Chen,Huaming Li,Yong Gao
In this work, a novel strategy for the construction of injectable self-healing nanocomposite (NC) hydrogels dominated by reversible boronic ester bonds was demonstrated. Specifically, NC hydrogels were constructed by the solution-mixing of N,N -dimethylacrylamide- stat -3-acrylamidophenylboronicacid statistical copolymers (PDMA- stat -PAPBA) and poly(glycerolmonomethacrylate) (PGMA) chains grafted cellulose nanocrystals (CNC- g -PGMA). Rheology analysis indicated the as-constructed NC hydrogel displayed about 7-fold increase in the storage modulus with a low CNCs loading level of 1.43 wt% in comparison with PGMA/PDMA- stat -PAPBA hydrogel without CNCs. Furthermore, the mechanical strength of the CNC- g -PGMA/PDMA- stat -PAPBA hydrogel was far superior to that of its PGMA/PDMA- stat -PAPBA/CNCs hydrogel counterpart, in which PGMA chains were not covalently grafted on the surfaces of CNCs. Due to reversible boronic ester bonds cross-linking networks, CNC- g -PGMA/PDMA- stat -PAPBA NC hydrogel exhibited excellent self-healing and injectable properties as well as pH/glucose responsive sol-gel transitions. Good biocompatibility was also demonstrated through in vitro cytotoxicity tests.
研究领域:
材料科学
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纳米技术
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生物医学工程
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SEPARATION AND PURIFICATION TECHNOLOGY
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2025
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IF 9
Xingrui Tang,Qiang Xian,Shibing Zuo,Yiyang Zeng,Hao Li,Jun Wen
Seawater uranium extraction via adsorption technology can effectively alleviate the shortage of terrestrial uranium resources and ensure a steady, sustainable supply of essential uranium. Most uranium adsorbents face challenges like inefficient ion transport, salt shrinkage and marine biofouling. Thus, we innovatively constructed zwitterionic marine-adaptive porous cryogel adsorbents (PAO-SBMA) for uranium extraction from seawater. The incorporation of zwitterionic sulfobetaine methacrylate (SBMA) not only regulates the hierarchical pore architecture of porous adsorbents but also imparts superhydrophilicity, antifouling capability, and salt-shrinkage resistance. Additionally, density functional theory (DFT) calculations, FT-IR and XPS spectra jointly prove that the sulfonic acid groups perform as effective synergistic coordinating groups, and facilitate the efficient adsorption of uranium by amidoxime-based adsorbents. Through this “triple enhancement” strategy, the PAO-SBMA-1.5 (m AN /m SBMA = 1.5) exhibits remarkable uranium adsorption performance. Its adsorption capacity is approximately three times that of the polyamidoxime (PAO), reaching 1595.90 mg/g. After 21 days of test in natural seawater, PAO-SBMA-1.5 demonstrates a high uranium extraction capacity of 13.88 mg/g, and its extraction efficiency has reached as high as 84 %. The zwitterion-based adsorbent is poised to become a promising candidate for implementation in marine uranium extraction operations.
研究领域:
环境科学
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材料化学
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核能
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海洋资源利用
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吸附技术
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