CARBOHYDRATE RESEARCH
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2024
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IF 2.4
Fang Wen,Yao Liu,Hehe Yang,Xu Yan,YanDong Zhang,Zhimei Zhong
In this study, carboxylic acids compounds were grafted onto chitooligosaccharides to prepare seven phenyl/indolyl-acyl chitooligosaccharides derivatives. The structures of the derivatives were characterized by IR spectroscopy, 13 C NMR and elemental analysis. Meanwhile, antioxidant activities in vitro of the novel derivatives were analyzed. Compared to COS and carboxylic acid, the derivatives showed higher scavenging capacity for superoxide anion and DPPH radicals, with scavenging rates of 59.39% and 94.86%, respectively. The hydroxyl radical scavenging ability of the derivatives was only 18.89%. The antifungal activities of chitooligosaccharide derivatives against Diaporthe batatas and Phytophthora capsici were studied by the growth rate method. Compared with chitooligosaccharide itself, derivatives were inhibited by 97.77% and 100%. The above results showed that chitooligosaccharide derivatives have good biocompatibility and can be used in food, agriculture and medicine.
研究领域:
生物化学
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食品科学
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农业科学
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DOI
PubMed
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
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2014
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IF 3.208
Guan-Lin Chen,Song-Gen Chen,Ying-Ying Zhao,Chun-Xia Luo,Juan Li,Yong-Qing Gao
In order to provide new information on the antioxidant activities before and after in vitro digestion of selected fruits for consumers, nutritionists, and food policy makers, total phenolic contents and antioxidant capacities of 33 fruits were evaluated by Folin–Ciocalteu method, 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity assay (DPPH), Ferric reducing antioxidant power assay (FRAP), and ABTS •+ radical cation scavenging activity (ABTS) assay, respectively. The correlations between DPPH, FRAP, ABTS values and total phenolic contents were also evaluated. The results showed that different fruits had diverse antioxidant capacities and the variation was very large. Before the gastric and duodenal phases of in vitro digestion, there was a high positive correlation between the DPPH, FRAP, ABTS values, and total phenolic contents. After the digestion, however, the correlations between the results and the total phenolic contents were poor. Four fruits, plum (sanhua) ( Prunus salicina Lindl), red bayberry ( Myrica rubra (Lour.)), plum (green) ( P. salicina Lindl), and mango (shuixian) ( Mangifera indica Linn) showed the strongest antioxidant activities they were before or after the digestion, which implied that these fruits were important natural sources for preventing oxidative stress diseases.
研究领域:
食品科学
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营养学
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生物化学
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DOI
高影响因子
RSC Advances
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2021
·Vol. 11
·期 53
·33219-33234
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IF 3.361
Zhen Wu,Ruiping Gao,Hong Li,Yongde Wang,Yang Luo,Jiang Zou,Bo Zhao,Shiqi Chen
The combinatorial use of dietary jujube (Ziziphus jujuba) and ginger play a critical role in traditional Chinese medicines, folk medicine and dietary therapy. Joint effects were investigated from the viewpoint of the antioxidant (scavenging DPPH˙) and antitumor activities (against SW620 cells) of jujube polysaccharides and ginger 6-gingerol (G6G) alone and in combination. Jujube polysaccharides were extracted, purified, and characterized, and then their inhibiting and apoptotic effects alone and in combination with G6G were evaluated by the cytological tests, including Cell Counting Kit-8, colony-forming, Annexin V-FITC and propidium iodide, TdT-mediated dUTP nick end labeling (TUNEL) staining, and cell cycle assays. Results showed that the purified polysaccharide fraction (ZJPs-II) with average molecular weight of 115 kDa consisted of arabinose, rhamnose, glucose, xylose, and galactose. ZJPs-II and G6G alone dose-dependently scavenged DPPH˙ and inhibited the proliferation of SW620 cells, while their combination showed synergistic interactions (all combination index < 1). The studies further demonstrated that ZJPs-II and G6G alone reduced the cell colony-formation, induced apoptosis and arrested the cell-cycle at G2/M phase, while their combination achieved better effects and significantly arrested the growth at the G0/G1 phase. Collectively, our findings suggest enhancing the intake of jujube polysaccharides and G6G in a combinatorial approach for maintaining health and preventing cancer.
研究领域:
药理学
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肿瘤学
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营养保健品
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传统中药
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
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2023
·Vol. 24
·期 7
·6247
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IF 5.6
Shifan Zhao,Hualei Xue,Yijiong Tao,Kai Chen,Xiao Li,Mi Wang
A novel water-soluble acidic heteropolysaccharide, called PPL-1, was purified fromPueraria lobata. PPL-1 had an average molecular weight of 35 Kad, and it was composed of glucose, arabinose, galactose and galacturonic acid (6.3:0.8:0.8:2.1). In accordance with methylation and nuclear magnetic resonance analyses, PPL-1 primarily consisted of (1→2)-linked α-Araf, (1→4)-linked α-Glcp, (1→)-linked β-Glcp, (1→6)-linked α-Glcp, (1→3,6)-linked α-Galp, (1→)-linked β-GalpA and (1→4)-linked α-GalpA. In terms of bioactivities, PPL-1 exhibited remarkable scavenging ability towards DPPH (1,1-Diphenyl-2-picrylhydrazyl) radicals and moderate activity by enhancing the proliferation rate of RAW 264.7 cells by approximately 30% along with the secretion of NO. This work demonstrates that PPL-1 can be a potential source of immunoenhancers and antioxidants.Keywords:Pueraria lobata;polysaccharide;structure;bioactivity
研究领域:
生物化学
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药理学
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免疫学
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分子生物学
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DOI
PubMed
高影响因子
JOURNAL OF AOAC INTERNATIONAL
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2025
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IF 1.7
Tang Dianchao,Jin Chen,Gan Liqin,Zhong Lanlan,Wang Zexi,Liu Qiqiong,Yang Yiling,Liu Ling,Huang Huilian
BackgroundTinosporae Radix is a valuable plant with applications in medicinal and edible fields. Alkaloids are one of the main active components of Tinosporae Radix, showing prominent antioxidant activity and great potential in healthcare fields.ObjectiveResponse surface method was applied to determine the optimal enrichment process of total alkaloids of Tinosporae Radix (TRTA). Antioxidant alkaloid components were screened by characteristic chromatogram, antioxidant analysis and molecular docking, so as to provide the basis for Tinosporae Radix to develop as a medicinal and edible homologous plant and antioxidant products.MethodsIn this study, a single-factor investigation experiment and a response surface optimization experiment were used to determine the optimal enrichment process of D101 macroporous resin. High-performance liquid chromatography (HPLC) was employed to establish the characteristic chromatogram of TRTA, after which chemometrics was applied to evaluate the data. The antioxidant activity of TRTA was assessed using DPPH and ABTS assays. Grey relational analysis was applied to explore the spectrum-effect relationship, and molecular docking techniques were combined to predict targets of antioxidant activity.ResultsThe best enrichment process of total alkaloids is presented as follows: water removal 3 BV, 10% ethanol removal 3 BV, and 30% ethanol elution 3 BV. And all four identified alkaloid components contributed to the antioxidant activity of TRTA, potentially processing binding interactions with multiple antioxidant protein targets, including TNF, NO-1 and XOD.ConclusionsAlkaloids significantly contributed to the antioxidant activity of TRTA, with columbamine and palmatine playing particularly prominent roles. And the antioxidant activity of TRTA is likely exerted through a complex multi-component, multi-target mechanism, involving the coordinated action of various alkaloid components and multiple biological targets.HighlightsThe optimized enrichment process demonstrated remarkable efficiency in concentrating total alkaloids from Tinosporae Radix. This study contributes significantly to the formulation of its quality control methods.
研究领域:
植物化学
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分析化学
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药理学
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计算生物学
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DOI
PubMed
高影响因子
FITOTERAPIA
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2024
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IF 2.5
Meihong Liu,Yongxia Jia,Haihui Xie
Michelia champaca L. (Magnoliaceae) was cultivated in large scale for flowers as cosmetic raw materials, whereas the value of its leaves remains to be discovered. Our chemical study on the leaves yielded four new flavonol diglycosides, champaflavosides A–D ( 1 – 4 ), together with twenty-three known flavonoid glycosides ( 5 – 27 ). Their structures were determined by spectroscopic and chemical methods. Compounds 5 – 21 and 23 – 27 were not previously reported from the genus Michelia , and kaempferol 3- O -rutinoside ( 22 ) was obtained from this species for the first time. All the compounds were evaluated for antioxidant activity by four in vitro assays. Compounds 3 – 12 and 20 showed more potent 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity than l -ascorbic acid ( l -AA). Compounds 2 – 23 , 25 , and 27 exhibited 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) radical cation scavenging activity superior to l -AA. The ferric reducing antioxidant powers (FRAP) of compounds 2 – 13 , 17 , and 19 were higher than l -AA. Further, eighteen compounds demonstrated cellular reactive oxygen species (ROS) scavenging activity, of which champaflavoside D ( 4 ), rhamnetin 3- O -neohesperidoside ( 8 ), quercetin 3- O -(6- O - E - p -coumaroyl)-neohesperidoside ( 9 ), and liquiritin ( 27 ) were more potent than curcumin. The results revealed that the renewable leaves of M. champaca are a rich source of flavonoids and antioxidants.
研究领域:
药用植物学
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生物化学
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植物化学
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DOI
PubMed
高影响因子
SOUTH AFRICAN JOURNAL OF BOTANY
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2021
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IF 2.315
Si-Yu Zhong,Hong-Yu Jiang,Li-Ping Wu,Meng-Ying Liu,Ya-Rui Yu,Cheng-Rong Li,Jing Huang
A new neolignan, (-)-(7 S , 8 R )-3‑methoxy-4', 7-epoxy-8, 5'-neolignane-5, 9, 3', 9′-tetraol ( 1 ) and a new biphenylpropanoid, 1, 2-epoxy-1-(3', 4'-dihydroxyphenyl-)-3-(2'', 3'', 5''-trihydroxyphenyl-) propane ( 2 ), together with eight known compounds ( 3-10 ), were isolated from the 95% EtOH extract of the leaves of Cupressus funebris Endl . Their structures were determined by spectroscopic methods including NMR, circular dichroism (CD) spectra and comparison of the NMR data with reported in references. The antioxidant activities of all compounds were evaluated by DPPH, ABTS, and FRAP methods. Compounds 2 (IC 50 : 33.98 ± 0.11 μM), 3 (IC 50 : 32.97 ± 0.63 μM), 8 (IC 50 : 32.07 ± 1.57 μM), and 9 (IC 50 : 33.30 ± 2.29 μM) showed stronger DPPH free radical scavenging activity than l -ascorbic acid (IC 50 : 64.31 ± 1.32 μM). Except for 4 , the other compounds showed ABTS radical scavenging abilities comparable to l -ascorbic acid (54.91 ± 1.18 μM) with the IC 50 ranged from 24.42 ± 1.78 μM to 138.70 ± 13.58 μM. Compound 3 (0.72 ± 0.02 mmol/g) showed the same ferric reducing antioxidant power (FRAP) as l -ascorbic acid (0.83±0.02 mmol/g).
研究领域:
药用植物学
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天然产物化学
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生物化学
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DOI
高影响因子
JOURNAL OF APICULTURAL RESEARCH
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2023
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IF 1.9
Olaoye Solomon Balogun,Anthonia Olufunke Oluduro,Zhiqiang Liu
Volatile oils of propolis obtained from Ondo (Ondo town) and Osun (Ile-Ife) States in Rain forest region of southwest Nigeria were extracted using the hydrodistillation method. The oils were analysed on GC/MS and investigated for DPPH scavenging activities, antibacterial potentials, and inhibitory activities on lipase and alpha glucosidase enzymes. Spectrophotometric methods were used for the DPPH scavenging and enzyme’ inhibitions assays while the agar well diffusion method was employed for the antibacterial test. GC/MS analysis of essential oils obtained from Ondo (BPO) and Ile-Ife (BPI) indicated 80 (97.82%) and 66 (99.31%) compounds respectively. Upon classification, both oils were rich in monoterpenes, sequisterpenes and non-isoprenoid hydrocarbons. Principal chemical components of BPO included trans-α-bergamotene (9.08%), α-copaene (5.83%), cadina-1,4-diene (4.04%), 2-methyl-5-propionylfuran (3.43%), 1,5,9,9-tetramethyl-1,4,7-cycloundecatriene (3.52%), benzaldehyde (2.98%), sulcatone (2.95%), γ-muurolene (2.51%), caryophyllene (2.40%), α-bisabolol (2.09%), α-eudesmol (2.07) and α-curcumene (2.06%) while those of BPI were trans-α-bergamotene (10.14%), citral (5.44%), α-copaene (5.25%), cadina-1,4-diene (5.09%), melonal (3.69%), sulcatone (3.56%), farnesol (2.71%), 3,5,9-trimethyl-deca-2,4,8-trien-1-ol (2.66%), α-curcumene (2.64%), 1,5,9,9-tetramethyl-1,4,7-cycloundecatriene (2.20%), and α-muurolene (2.20%). Both oils inhibited α-glucosidase and had DPPH scavenging activities better than the standards, however, anti-lipase activity was observed for BPI only. The oils also demonstrated broad spectrum of antibacterial activities.The volatile components of Nigerian bee propolis are being investigated for the first time, and the plethora of biological activities resulting from their diverse chemical composition showed that bee propolis could be a potential source for drug discovery.
研究领域:
药理学
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植物化学
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微生物学
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高影响因子
MOLECULES
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2023
·Vol. 28
·期 17
·6176
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IF 4.6
Jianfa Wu,Lei Li,Chang Liu,Chunyi Li,Ying Cui,Weixing Ding,Jing Zhang,Leiling Shi
Two new compounds named 4,4′-bis(β-D-glucopyranosyloxy)biphenyl (1) and spirostane-25(27)-en-2α,3β-diol-3-O-β-D-xylopyranosyl(1→3)-β-D-glucopyranosyl(1→4)-β-D-galactopyranoside (2) were isolated from n-butanol extraction part of 80% ethanol extract of Allii Macrostemonis Bulbus. Alongside these, ten known compounds (3–12) were also identified, including a flavonoid glycoside (3), seven steroids (4–10), a nucleoside (11), and a phenylpropanoid glycoside (12) were found. Notably, compounds3–6were isolated from this plant for the first time. The structures of all compounds were confirmed using high-resolution electrospray ionization mass spectrometry (HR-ESI-MS), 1D, and 2D NMR spectroscopy. Some of these compounds showed strong antioxidant activity, and compound1demonstrated the most potent reduction of ferric ions (Fe3+) with an IC50value of 0.59 ± 0.18 mg/mL. Compounds2and3exhibited the highest scavenging activity against superoxide anion radicals (O2−·) with an IC50value of 0.02 ± 0.01 mg/mL. Additionally, compound3displayed substantial scavenging activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) with IC50values of 0.21 ± 0.17 mg/mL and 0.02 ± 0.01 mg/mL, respectively. The discovery of these two new compounds is a reference for identifying Allii Macrostemonis Bulbus quality markers. Moreover, their exceptional antioxidant activity offers a promising avenue for uncovering novel natural antioxidants.
研究领域:
药用植物学
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天然产物化学
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药物开发
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DOI
PubMed
高影响因子
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2023
·
IF 4.1
Bowei Du,Chao Zhang,Guiya Deng,Shuai Zhang,Shiqi Wang,Yuepeng Guan,Yaqin Huang
Background Nowadays, the prevalence of oxidative stress-related chronic diseases is increasing. The identification of novel antioxidant collagen-peptides to counteract oxidative stress for individuals’ health have gained significant intention. Results In this study, the collagen-peptides with antioxidant activities were separated and identified by ion chromatography (IEC), reversed-phase high-performance liquid chromatography (RP-HPLC), and liquid chromatography-tandem mass spectrometry (LC-MS/MS). The identified antioxidant collagen-peptides were further screened by molecular docking for Keap1-targeted peptide inhibitors and their theoretical interaction mechanisms were investigated. Four novel antioxidant collagen-peptides, GPAGPIGPVG, GPAGPpGPIG, ISGPpGPpGPA and IDGRPGPIGPA with high binding affinity to Keap1 were selected. Molecular docking results demonstrated that the putative antioxidant mechanism of four antioxidant collagen-peptides contributed to their blockage of Keap1-Nrf2 interactions. The results of antioxidant activity of four antioxidant collagen-peptides proved that IDGRPGPIGPA exerted a high scavenging capacity to DPPH and ABTS free radicals, while GPAGPpGPIG improved the resistance of cells to hydrogen peroxide (H 2 O 2 ) induced oxidative damage by promoting the activation of intracellular antioxidant enzymes and the production of reduced glutathione in human hepatoma (HepG2) cells. Conclusion The antioxidant collagen-peptides (GPAGPIGPVG, GPAGPpGPIG, ISGPpGPpGPA and IDGRPGPIGPA) will be developed as the novel functional food for human health in the near future. This article is protected by copyright. All rights reserved.
研究领域:
药理学
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生物化学
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分子生物学
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细胞生物学
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食品科学
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DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
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2025
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IF 6.6
Jiandong Shen,Bijiang Zhong,Lijun Fu,Bingyan Liu,Wenshui Xia,Qixing Jiang
Aquatic animals are vital source for peptides, while information on antioxidant peptides of Chinese softshell turtle is scarce. The objective of this study was to optimize the production of turtle protein hydrolysate (PTH) by proteinase hydrolysis and evaluate its functional property and antioxidant activity. Results revealed that TPH from turtle protein was hydrolysed using protamex. Under the optimum condition (enzyme-substrate ratio of 3500 U/g, pH 7.0, temperature of 50 °C, and 4 h), TPH had the highest 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, with the degree of hydrolysis (DH) of 21.27 ± 1.32%. Obtained TPH contained a high percentage of essential amino acids and its molecular weight of<3000 Da accounted for 99.61%. The solubility of TPH was more than 80% when pH was below 4.0 or above 5.0, and there was no significant difference in its solubility when temperature was 40–100 °C. Meanwhile, TPH exhibited higher emulsifying activity index and the emulsion stability index at pH 7.0 and 10.0. Antioxidant activity studies showed that DPPH scavenging activity, reducing power and ferrous chelating activity of TPH were attained depending on the concentration of hydrolysate. All results indicated that the functional and antioxidant properties of TPH could be beneficial for its application in functional food.
研究领域:
食品科学
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生物化学
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高影响因子
Food Bioscience
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2020
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IF 3.067
Xiao-Hui Yao,Yu-Shi Shen,Run-Ze Hu,Ming Xu,Jia-Xing Huang,Chen-Xi He,Fu-Liang Cao,Yu-Jie Fu,Dong-Yang Zhang,Wei-Guo Zhao,Li Liu,Tao Chen
Mulberry is a traditional crop that has been grown in China for thousands of years. While leaves and fruits of the mulberry have been consumed, mulberry seed use has been less extensive. The goal of this study was to investigate the value of the seeds. Seven cultivars of mulberry were investigated. Oil was extracted from seeds, and the oil content was determined. The primary components of the oils were analyzed using gas chromatography . It was found that all the mulberry cultivars contained high linoleic acid contents, suggesting that mulberry seed may be a potential resource of linoleic acid. The total phenolic and flavonoid contents were also compared among different mulberry seed oils . The physical and chemical parameters, as well as the antioxidant activity of the mulberry seed, were determined. Principal component analysis indicated that the ferric ion reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl free radical-scavenging activity (DPPH) of mulberry seed oil may be due to high phenolic compounds and linoleic acid. The cultivars Dongsheng, Fengtian, and Guisang had the highest antioxidant activities (FRAP, DPPH), and it was proposed that these cultivars would produce the highest quality oil for use as an ingredient for healthcare or functional food use.
研究领域:
食品科学
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营养学
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农业
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生物化学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）