Journal of Functional Foods
·
2015
·
IF 3.574
Guan-Lin Chen,Song-Gen Chen,Ying-Qing Xie,Fu Chen,Ying-Ying Zhao,Chun-Xia Luo,Yong-Qing Gao
In order to provide new information on the antioxidant activities before and after in vitro digestion of the selected flowers for consumers, nutritionists, and food policy makers, total phenolic and flavonoid contents and antioxidant capacities of 23 edible flowers were evaluated. In addition, some bioactive components in the flowers were also identified and quantified by ultra performance liquid chromatography (UPLC). The correlations between 2, 2-diphenyl-1-picrylhydrazylradical scavenging activity assay (DPPH), Ferric reducing antioxidant power assay (FRAP), and ABTS •+ radical cation scavenging activity assay (ABTS) values and total flavonoid contents and total phenolic contents were also evaluated. Three flowers, Osmanthus fragrans (Thunb.) Lour, Paeonia lactiflora Pall, and Rosa rugosa Thunb (purple) showed the strongest antioxidant activities before or after the digestion, which implied that these flowers are important natural sources for the prevention of oxidative stress diseases.
研究领域:
食品科学
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营养学
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药理学
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植物科学
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产品关联
DOI
高影响因子
ChemistrySelect
·
2022
·Vol. 7
·期 42
·e202203493
·
IF 2.307
Xiang Yu,Mengyu Liu,Zaiheng Huang,Yanzhi Tao,Hao Yang,Hongbin Gan,Guangzai Nong
Graphical Quercetin and selenite were combined to form a quercetin selenium complex at 100°C. The mass of the product was 30.3 % of the mass of quercetin and the structure was analyzed in depth. The complex had a DPPH antioxidant activity of 97.91 %, and the prepared skin cream containing 1 % of the complex had an antioxidant activity of 82.4 %. Flavonoid complexes have more biological activities than parent flavonoids, including antioxidant, anticancer, antibacterial, and many others. Selenium is an essential trace element for human body. Therefore, a quercetin-Se complex was synthesized and analyzed in this paper, getting the results as following: (1) A light- yellow product was obtained with the max yield of 30.30 %. (2) The complex molecule contains two quercetin units and a selenoxyl group, called selenious acid acyl diquercetin. (3) It has a strong antioxidant activity with a maximum DPPH scavenging rate of 97.71 %. In addition, we prepared a skin cream containing 1 % quercetin selenium complex, and it has excellent antioxidant activity with a DPPH scavenging rate of 82.4 %. Therefore, the generated selenious acid acyl diquercetin is a potential antioxidant material for skin care products.
研究领域:
化学
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生物学
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药学
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DOI
高影响因子
Frontiers in Nutrition
·
2020
·
IF 3.365
Luo Xuguang,Wang Jin,Chen Haiqiang,Zhou Aimei,Song Mingyue,Zhong Qingping,Chen Hanmin,Cao Yong
Finger citron (Citrus medica L. var. sarcodactylis Swingle) is a traditional Chinese herb and considered as a healthy food. Flavonoids are the major bioactive substances in Finger citron. In this study, the major flavonoids of finger citron (FFC) were purified with AB-8 macroporous resins, and then three of them were identified as diosmetin-6-8-di-C-glucoside, hesperidin and diosmetin-6-C-glucoside, and other two were preliminarily inferred as limocitrol 3-alpha-l-arabinopyranosyl-(1->3)-galactoside and scutellarein 4′-methyl ether 7-glucoside by high-performance liquid chromatography and ultraperformance liquid chromatography to quadrupole time-of-flight mass spectrometry. Further, their antioxidation and antiaging activities were determined in vitro and in vivo. In vitro, chemical assays revealed that the purified FFC had strong antioxidative activity as demonstrated by its strong DPPH (2,2-diphenyl-1-picrylhydrazyl) and ABTS [2,2-azinobis (3-ethyl-benzothiazoline-6-sulphonic acid) diammonium salt] radical scavenging activities and ORAC (oxygen radical absorbance capacity). In vivo, the purified FFC significantly increased the mean and maximum lifespan of Caenorhabditis elegans by 31.26 and 26.59%, respectively, and showed no side effects on their physiological functions. Under normal and oxidative stress conditions, purified FFC reduced the accumulation of reactive oxygen species (ROS) and malondialdehyde, while increased superoxide dismutase (SOD) and catalase (CAT) enzyme activities in C. elegans. Together, we successfully identified three major substances in purified FFC of finger citron and determined the excellent antiaging activity of FFC, which is attributed to its strong antioxidative activity and effect on homeostasis of ROS.
研究领域:
植物化学
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药理学
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生物化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2018
·
IF 3.412
Dongmei Li,Xiaokang Na,Haitao Wang,Yisha Xie,Shuang Cong,Yukun Song,Xianbing Xu,Bei-Wei Zhu,Mingqian Tan
Food-borne nanoparticles have received great attention because of their unique physicochemical properties and potential health risk. In this study, carbon dots (CDs) formed during one of the most important chemical reactions in the food processing field, the Maillard reaction from the model system including glucose and lysine, were investigated. The CDs purified from Maillard reaction products emitted a strong blue fluorescence under ultraviolet light with a fluorescent quantum yield of 16.30%. In addition, they were roughly spherical, with sizes of around 4.3 nm, and mainly composed of carbon, oxygen, hydrogen, and nitrogen. Their surface groups such as hydroxyl, amino, and carboxyl groups were found to possibly enable CDs to scavenge DPPH and hydroxyl radicals. Furthermore, the cytotoxicity assessment of CDs showed that they could readily enter HepG2 cells while causing negligible cell death at low concentration. However, high CDs concentrations were highly cytotoxic and led to cell death via interference of the glycolytic pathway.
研究领域:
食品科学
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生物医学
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纳米材料
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产品关联
DOI
PubMed
高影响因子
Frontiers in Nutrition
·
2022
·
IF 6.59
Ji Xiaolong,Guo Jianhang,Pan Feibing,Kuang Fengjun,Chen Haiming,Guo Xudan,Liu Yanqi
A novel neutral polysaccharide designated as PAP1b was isolated from Areca catechu L. by hot water extraction, ethanol precipitation, and column chromatography. PAP1b was mainly composed of mannose, galactose, xylose, and arabinose in a ratio of 4.1:3.3:0.9:1.7, with an average molecular weight of 37.3 kDa. Structural characterization indicated that the backbone of PAP1b appeared to be composed mainly of → 6-β-Manp-(1 →, → 4)-α-Galp-(1 → and → 3,6)-β-Manp-(1 →) residues with some branches, and terminal of (1 →)-linked-β-Manp residues. The results of bioactivity experiments showed that PAP1b had antioxidant in vitro, esspecially on scavenging DPPH and hydroxyl radicals. Therefore, the polysaccharide from Areca catechu L. could be used as a potential antioxidant in functional food.
研究领域:
植物化学
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食品科学
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天然产物化学
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DOI
高影响因子
MOLECULES
·
2018
·Vol. 23
·期 10
·2663
·
IF 3.098
Tian Chai,Xiao-Bo Zhao,Wei-Feng Wang,Yin Qiang,Xiao-Yun Zhang,Jun-Li Yang
Gx-50 is a bioactive compound for the treatment of Alzheimer’s disease (AD) found in Sichuan pepper (Zanthoxylum bungeanum). In order to find a stronger anti-AD lead compound, 20 gx-50 (1–20) analogs have been designed and synthesized, and their molecular structures were determined based on nuclear magnetic resonance (NMR) and mass spectrometry (MS) analysis, as well as comparison with literature data. Compounds1–20were evaluated for their anti-AD potential by using DPPH radical scavenging assay for considering their anti-oxidant activity, thioflavin T (ThT) fluorescence assay for considering the inhibitory or disaggregate potency of Aβ, and transgenicDrosophilamodel assay for evaluating their rescue effect on memory loss. Finally, compound13was determined as a promising anti-AD candidate.Keywords:Alzheimer’s disease;gx-50;DPPH assay;ThT assay;transgenicDrosophilamodel assay
研究领域:
药理学
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神经科学
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化学
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产品关联
DOI
PubMed
高影响因子
PROCESS BIOCHEMISTRY
·
2024
·
IF 3.7
Rundong Yang,Siyan Liu,Feifei Wang,Shuyi Li,Na Zhang,Zhenzhou Zhu
Laminaria polysaccharides (LP) have been shown to effectively stabilize selenium nanoparticles (SeNPs), forming LP–SeNP complexes with enhanced bioactivity. However, the correlation between their bioactivity and physicochemical properties remains inadequately explored. This study used chemical reduction with LP as stabilizer to investigate how LP-to-selenium mass ratio (LPSMR), reaction time, and temperature influence particle size, selenium content, and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity of LP–SeNPs. The results showed that enhanced DPPH scavenging correlated with higher selenium content and smaller particle size, primarily modulated by LPSMR and temperature. Under optimal conditions (LPSMR of 1; temperature of 25°C), the resulting LP–SeNPs exhibited uniform morphology with a particle size of 81.41 nm and selenium content of 653.91 mg/g. This stability was achieved through non-covalent interactions between LP and SeNPs, providing superior light and acid resistance compared to unmodified SeNPs. Notably, LP–SeNPs showed synergistic antioxidant effects, with lower half-maximal inhibitory concentration (IC 50 ) values for scavenging DPPH, hydroxyl, and superoxide anion radicals than LP or SeNPs alone, and enhanced hypoglycemic activity. Cytotoxicity assays confirmed LP–SeNPs had reduced toxicity compared to Na 2 SeO 3 and selenopeptide. These findings provide insights into the structure-activity relationships of LP–SeNPs and support their potential application as antioxidant and hypoglycemic agents.
研究领域:
纳米技术
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药学
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材料科学
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生物化学
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产品关联
DOI
高影响因子
Frontiers in Nutrition
·
2021
·
IF 6.576
Yuan Li,Chu Qian,Wu Xiaoyun,Yang Bei,Zhang Wei,Jin Wengang,Gao Ruichang
Research has shown that cartilage containing chondroitin sulfate and protein presents versatile bioactivities. Chondroitin sulfate in cartilage is beneficial to activate the immune system while the protein/peptide has not been fully understood. The current study investigated the antioxidant and anti-inflammatory properties of ethanol-soluble hydrolysates of sturgeon cartilage (ESCH) prepared through hot-pressure, enzymatic hydrolysis and ethanol extraction. UV spectrum, IR and agarose gel electrophoresis results suggested the successful exclusion of chondroitin sulfate from peptides. Nitric oxide (NO) floods in cells activated by inflammation. It was inhibited when administrated with ESCH. To further explain the observed anti-inflammatory activity, ESCH was separated with Sephadex G-15 into 3 components, among which F3 showed a higher NO inhibition rate and significantly reduced the production of the proinflammatory cytokine IL-6. In addition, the yield of IL-10 increased. Western blotting suggested that F3 downregulated the NO content and IL-6 level by suppressing Mitogen-activated protein kinases (MAPK) channels. Moreover, both ESCH and F3 showed DPPH and ABTS free radical scavenging abilities which was possibly related to the anti-inflammatory property. These results indicated that ESCH behaved anti-inflammatory and antioxidant activities. Cartilage may be a good source to produce anti-inflammatory peptides.
研究领域:
生物化学
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免疫学
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药理学
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产品关联
DOI
PubMed
高影响因子
Food & Function
·
2022
·Vol. 13
·期 7
·3946-3956
·
IF 6.317
Zhe Wang,Chunhai Li,Xi He,Kang Xu,Zhipeng Xue,Ting Wang,Zhenshang Xu,Xinli Liu
The effect of Platycodon grandiflorum (PG) on colitis and its underlying mechanism were rarely studied. In this study, Lactobacillus rhamnosus 217-1 was used to ferment PG roots, and the concentrations of platycodin-D, flavonoids, and polyphenols and the DPPH free radical scavenging rate were significantly increased. Treatment with a PG root fermentation broth (PGRFB) could reduce dextran sulfate sodium (DSS) induced ulcerative colitis (UC) in mice. Meanwhile, the PGRFB significantly reduced the content of inflammatory factors in mouse serum and the expression of inflammatory factor mRNA in the intestinal tract, regulated the polarization of M1/M2 macrophages, and increased the expression of tight junction protein mRNA in intestinal epithelial cells. In summary, it was proved that the PGRFB could inhibit the nuclear factor kappa B (NF-κB) signaling pathway and the expression of Nod-like receptor protein 3 (NLRP3) inflammasomes by activating AMP-activated protein kinase (AMPK) and lowering the release of pro-inflammatory cytokines.
研究领域:
药理学
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免疫学
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微生物学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD SCIENCE
·
2015
·Vol. 80
·期 6
·C1162
·
IF 1.696
Hai-wei Jiang,Hong-yan Li*,Cheng-wei Yu,Ting-ting Yang,Jiang-ning Hu,Rong Liu,Ze-Yuan Deng*
en Isobolographic analysis was used to assess the antioxidant interactions (synergism, addition, and antagonism) of 4 common vegetables (tomato [T], carrot [C], eggplant [E], and purple potato [P]). The lipophilic (L) extracts of T and C (main carotenoids), the hydrophilic (H) extracts of E and P (main phenolics) were mixed by the certain ratios (1:9, 3:7, 1:1, 7:3, 9:1, w/w) and their antioxidant activities were investigated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2’-Azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) radical scavenging assays, respectively. Most of the binary mixtures (LC-HE, LC-HP, HE-HP, LT-HE, and LT-HP combinations) showed the synergistic antioxidant effects. In DPPH assay, the greatest antioxidant activity of vegetable combinations was 1:9 LT-HP (EC 50 : 2.45 ± 0.13 mg/mL), followed by 9:1 HE-HP (EC 50 : 3.62 ± 0.12 mg/mL) and 1:9 LC-HE (EC 50 : 3.74 ± 0.47 mg/mL). In ABTS assay, the greatest antioxidant activity of vegetable combinations was 9:1 HE-HP (EC 50 : 4.20 ± 0.10 mg/mL), followed by 7:3 HE-HP (EC 50 : 4.41 ± 0.63 mg/mL) and 1:1 HE-HP (EC 50 : 5.35 ± 0.85 mg/mL). Among these combinations, 1:1 LC-HE combination showed the highest synergistic antioxidant effects in DPPH assay (synergistic rate: 87.4%), and 7:3 LC-HE combination showed the highest synergistic antioxidant effects in ABTS assay (synergistic rate: 87.0%). The mixtures of phenolics and carotenoids with suitable ratios in vegetables effectively enhanced the synergistic antioxidant effects. Practical Application pt In this study, the results indicate that the combination of different vegetables with suitable ratios could improve the antioxidant activities through synergistic effects of phytochemicals. Also, isobolographic analysis could be well used in evaluating the antioxidant interactions between antioxidants.
研究领域:
食品科学
·
营养学
·
生物化学
·
植物化学
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产品关联
DOI
PubMed
高影响因子
CHEMISTRY & BIODIVERSITY
·
2023
·Vol. 20
·期 10
·e202300620
·
IF 2.9
Dang-Nan Zhou,Lu Han,Zhan Zhang,Yun-Long Wang,Xiao-Ping Zhang,Yun-Jie Wu,Gao-Lei Xi
Five psoralen derivatives were synthesized and the structures of them were characterized by 1 H-NMR, 13 C-NMR, and IR. The antioxidant properties of the compounds were tested by inhibiting the free radical-initiated DNA oxidation and scavenging the radical reaction. The results showed that the effective stoichiometric factors ( n ) of the compounds V and IV could reach 2.00 and 2.11 in the system of inhibiting the DNA oxidation reaction initiated by 2,2′-Azobis(2-methylpropionamidine) dihydrochloride (AAPH). In the inhibition of ⋅OH-oxidation of the DNA system, compounds I ~ V showed antioxidant properties. The thiobarbituric acid absorbance (TBARS) percentages of compounds IV and V were 76.19 % and 78.84 %. Compounds I ~ V could also inhibit Cu 2+ /GSH-oxidation of DNA, and all compounds exhibited good antioxidant properties except compound II (94.00 %). All the five compounds were able to trap diammonium 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonate) salt radical (ABTS + ⋅), 2,2-diphenyl-1-picrylhydrazyl radical (DPPH⋅) and 2,6-di-tert-butyl-alpha-(3,5-di-tert-butyl-4-oxo-2,5-cyclohexadien- p- tolylox radical (galvinoxyl⋅). The ability of compounds I ~ V to scavenge those free radicals can be measured by the k values. The k values ranged from 0.07 to 0.82 in scavenging ABTS + ⋅, galvinoxyl, and DPPH radicals, respectively. Graphical
研究领域:
化学
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药理学
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生物化学
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产品关联
DOI
PubMed
高影响因子
CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
·
2014
·Vol. 33
·期 1
·159-168
·
IF 0.684
Wang Xueqin,Xing Ronge,Liu Song,Yu Huahua,Li Kecheng,Chen Zuoyuan,Li Pengcheng
Mackerel ( Pneumatophorus japonic u s ) proteins were hydrolyzed by five proteases: trypsin, papain, neutrase, acid protease, and flavourzyme. The hydrolysate treated by neutrase exhibited the highest antioxidant activity. Response surface methodology (RSM) was employed to optimize the hydrolysis conditions in an effort to obtain a mackerel protein hydrolysate (MPH) with the highest DPPH radical scavenging activity. The MPH was fractioned using a series of ultrafiltration membranes and five fractions, namely, MPH-I (>10 kDa), MPH-II (10–2.5 kDa), MPH-III (1–2.5 kDa), MPH-IV (0.4–1 kDa), and MPH-V (below 0.4 kDa), were obtained. DPPH radical scavenging activity, reducing power, hydroxyl radical scavenging activity, and the lipid peroxidation inhibition capability of these fractions were evaluated. The fractions in molecular weights <2.5 kDa (MPH-III, MPH-IV, and MPH-V), which occupied 93.4% of the total fractions, showed the strongest antioxidant activity; and the antioxidant activities of the three fractions are similar to each other. Using SP Sephadex C-25 and Sephadex G-25 columns, eight fractions were obtained from the MPH (<2.5 kDa). The isolated peptide I (1 664 kDa) displayed the highest DPPH radical scavenging activity. Therefore, MPH is a potential source of antioxidant peptides.
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）