BIOCONJUGATE CHEMISTRY
·
2021
·
IF 4.774
Ruiyi Dai,Xiangrong Peng,Bi Lin,Danyang Xu,Ruichan Lv
In this research, a NIR II luminescence imaging and enhanced chemo-/photothermal therapy system of CuS–DOX–Nd/FA NPs for breast cancer and lymph node tracing under single 808 nm irradiation is proposed. Nd–DTPA molecular cluster with the NIR II imaging effect as the carrier was designed to load the ultrasmall CuS nanoparticles and chemotherapeutic drug doxorubicin hydrochloride (DOX). The composite probe is used for tumor lesion imaging and tracking the breast cancer sentinel lymph nodes with simultaneous chemo-/photothermal therapy (PTT) for breast cancer under the single 808 nm laser. This designed probe not only has high permeability and retention (EPR) targeting effect but also can respond to the tumor microenvironment (TME), realizing more precise and efficient release of DOX at the cancer focus. At the same time, CuS as a drug carrier has a good photothermal therapy effect (photothermal conversion efficiency: 27.9%). The serialized released chemotherapy DOX and synergistic PTT effect can be used to the treat the in situ breast cancer land and simultaneously kill the metastasis cancer. The system made the combined molecular clusters Nd–DTPA achieve NIR II imaging of tumor lesions of breast cancer and lymph node to obtain the integration of diagnosis of the transferred disease for better prognosis. The feasibility of the system had obvious tumor growth inhibition effect with NIR II imaging guided is verified by a series of in vitro and in vivo experiments.
研究领域:
肿瘤学
·
生物医学工程
·
纳米医学
·
分子影像学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2013
·
IF 5.008
Mengxin Zhang,Yuhua Cao,Yu Chong,Yufei Ma,Hailu Zhang,Zongwu Deng,Chunhong Hu,Zhijun Zhang
Magnetic resonance imaging (MRI) is a powerful and widely used clinical technique in cancer diagnosis. MRI contrast agents (CAs) are often used to improve the quality of MRI-based diagnosis. In this work, we developed a positive T1 MRI CA based on graphene oxide (GO)–gadolinium (Gd) complexes. In our strategy, diethylenetriaminepentaacetic acid (DTPA) is chemically conjugated to GO, followed by Gd(III) complexation, to form a T1 MRI CA (GO–DTPA–Gd). We have demonstrated that the GO–DTPA–Gd system significantly improves MRI T1 relaxivity and leads to a better cellular MRI contrast effect than Magnevist, a commercially used CA. Next, an anticancer drug, doxorubicin (DOX), was loaded on the surface of GO sheets via physisorption. Thus-prepared GO–DTPA–Gd/DOX shows significant cytotoxicity to the cancer cells (HepG2). This work provides a novel strategy to build a GO-based theranostic nanoplatform with T1-weighted MRI, fluorescence imaging, and drug delivery functionalities.
研究领域:
生物医学工程
·
材料科学
·
分子影像
·
癌症研究
·
纳米技术
✓ 已验证
DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2019
·Vol. 7
·期 18
·2938-2947
·
IF 5.047
Jing Wang,Yonghong Song,Mingxun Zhang,Zhensheng Wu,Yun-Jun Xu,Jun Lin,Daishun Ling,Youjing Sheng,Yang Lu,Qiang Wu
Combination chemotherapy has improved the life quality of patients suffering from ovarian cancer; however, drug resistance and late relapse lead to a high mortality rate. As a bioactive component in zedoary oil, curcumol has been widely used against gynecological tumors in the clinic. However, the bioavailability of curcumol is limited by its indissolubility in water. The design of nano-formulas is promising to improve traditional drugs in the clinic, which is beneficial for the treatment of malignant diseases, especially cancer. Herein, a liposomal curcumol nanocomposite was prepared for the theranostics of human primary ovarian cancer cells from solid tumor tissue in patients. In this uniform nanocomposite, curcumol and a frequently used magnetic resonance contrast agent, gadopentetate dimeglumine (Gd-DTPA), were effectively wrapped in the hydrophobic lipid bilayer and aqueous cavity of the liposome, respectively. Parallel assessments of the anti-tumor ability and mechanism of liposomal curcumol were performed on both SKOV3 and human primary ovarian cancer cells, and the results indicated the induction of apoptosis in ovarian cancer cells by this curcumol nanocomposite via the endoplasmic reticulum pathway. Liposomal curcumol has been further confirmed to inhibit the growth of SKOV3 human ovarian cancer xenografts on tumor-bearing nude mice. Moreover, this gadolinium-based liposomal curcumol nanocomposite exhibited excellent T1-weighted magnetic resonance imaging (MRI) capability for the efficient detection of xenograft ovarian cancer, leading to a novel multifunctional therapeutic agent.
研究领域:
药学
·
肿瘤学
·
纳米技术
·
医学影像
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2012
·
IF 5.856
Fengshan Shen,Hyundoo Hwang,Young Ki Hahn,Je-Kyun Park
This paper describes a new label-free cell separation method using a magnetic repulsion force resulting from the magnetic susceptibility difference between cells and a paramagnetic buffer solution in a microchannel. The difference in the magnetic forces acting on different-sized cells is enhanced by adjusting the magnetic susceptibility of the surrounding medium, which depends on the concentration of paramagnetic salts, such as biocompatible gadolinium diethylenetriamine pentaacetic acid (Gd–DTPA), dissolved therein. As a proof-of-concept demonstration, Gd–DTPA solutions at concentrations of 0–80 mM were applied to separate U937 cells from red blood cells (RBCs) and to distinguish two different-sized polystyrene (PS) beads (8 and 10 μm in diameter). By increasing the Gd–DTPA concentration from 0 to 40 mM, the separation resolution of PS beads was increased from 0.08 to 0.91. Additionally, we successfully achieved label-free separation of U937 cells from RBCs with >90% purity and 1 × 105 cells/h throughput using a 40 mM Gd–DTPA solution.
研究领域:
生物医学工程
·
细胞生物学
·
微流控技术
·
分析化学
✓ 已验证
DOI
PubMed
高影响因子
CHEMOSPHERE
·
2021
·
IF 7.086
Haris Muhammad,Ting Wei,Geng Cao,ShengHui Yu,XinHao Ren,HongLei Jia,Atif Saleem,Li Hua,JunKang Guo,Yongtao Li
The application of crops straw and biochar in trace metals remediation from the contaminated environment attracted more and more attention during the past decade. Although there has been some review work on the mechanism of trace metals stabilization by crops straw, the effects and mechanisms of interaction among soil indigenous-microbes and crops-straw for trace metal adsorption and stabilization is still unclear. In this study, the dynamic effects along with potential mechanisms of wheat-straw (WS), wheat-straw biochar (WBC) and biologically modified wheat-straw (BMWS) were conducted to investigate the adsorption, leaching behaviour, chemical fractions and bioavailability of cadmium (Cd). The results showed that the biosorption capacity (q e ) was most elevated in the BMWS treatment (14.42 mg g −1 ) as compared to WBC (6.28 mg g −1 ) and WS (4.20 mg g −1 ). The application of BMWS, WBC and WS at the rate of 3% significantly reduced Cd concentration in leachate to 53, 45 and 21% respectively, as compared to control. The addition of BMWS reduced the exchangeable Cd fraction resulted an increase in organic matter and carbonate bound Cd fraction in the soil. The DTPA extractable Cd was significantly decreased by 31.2 and 28.6% with the application of BMWS and WBC at 3% w/w respectively as compared to control. The research results may provide a novel perceptive for the development of functional materials and strategies for eco-friendly and sustainable trace metal remediation in contaminated soil and water by combination of straw and soil-indigenous microorganisms .
研究领域:
环境科学
·
土壤学
·
微生物学
·
污染控制
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Cancer
·
2024
·Vol. 15
·期 17
·5594-5604
·
IF 3.3
Cheng Yuanyuan,Gong Shushu,Li Qianqian,Shen Juan,Huang Hongjuan
Objective: To demonstrate the efficacy of photodynamic therapy (PDT) using hematoporphyrin derivative (HPD) nanomedicine in combination with conventional chemotherapy based on gadolinium-diethylenetriamine pentaacetic acid (Gd-DTPA) magnetic resonance imaging (MRI) for hepatocellular carcinoma (HCC) therapy.Methods: HPD nanomedicine was prepared, and the cytotoxicity of HPD nanomedicine at different concentrations on HCC cells and the half-maximal inhibitory concentration (IC50) were analyzed. Sixty HCC patients who visited our hospital from 2021 to 2023 were retrospectively analyzed. Patient data were analyzed, with 30 cases in control group (CG) receiving conventional chemotherapy for HCC, and 30 cases in observation group (OG) receiving conventional chemotherapy combined with HPD nanomedicine PDT. Gd-DTPA MRI was utilized to monitor the morphological and biological characteristics of the lesions in patients. After treatment completion, the long-term efficacy of patients and the levels of bcl-2 and bax proteins in primary HCC cells were evaluated.Results: The IC50 values of HPD on HepG2 cell proliferation and the cell inhibition rates gradually increased with increasing doses of HPD (50 μM, 25 μM, 12.5 μM, 6.25 μM, 3.13 μM, 1.56 μM, 0.78 μM). HPD exhibited great anti-proliferative effects on HepG2 cells at relatively low concentrations. The differences in expression rates of bcl-2 protein and bax protein between groups were considerable (P<0.05). There were neglectable changes in AST and ALT levels between the two groups before treatment, but they were markedly reduced after treatment versus before treatment (P<0.05), with OG showing considerably lower levels than CG after treatment (P<0.05). Additionally, patients in OG exhibited better survival rates after the course of treatment versus those in CG.Conclusion: This study demonstrates that the combination of conventional chemotherapy based on Gd-DTPA MRI with HPD nanomedicine PDT greatly improves the efficacy of treatment for HCC patients. This combined treatment strategy not only enhances therapeutic outcomes but also alleviates adverse reactions associated with conventional treatment, providing a novel approach for future research in the treatment of HCC.
研究领域:
肿瘤学
·
药理学
·
医学影像学
·
纳米技术
·
细胞生物学
✓ 已验证
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2021
·
IF 7.963
Hongyan Huang,Jinsong Chen,Shibin Liu,Shengyan Pu
In the present study, we investigated the responses of microbial respiration and community structure, enzyme activity and DTPA-extractable Pb within 60 days of incubation in soils treated with Pb and nano-ZnO. The results showed that when the concentration of nano-ZnO exceeded 10 mg/kg, the concentration of DTPA-extractable Pb significantly decreased by 10.6%–21.3% on the 60th day of the experiment. The addition of nano-ZnO decreased the Pb-contaminated soil pH from 6.18 to 6.08 at 7 days, which is part of the reason for the β-glucosidase activity change. Ten mg/kg nano-ZnO significantly reduced the qCO 2 value, which represented the microbial energy demand for the conversion of carbon sources into biomass. Nano-ZnO improved the microbial diversity and richness of some metal-tolerant bacteria at 60 days. The findings provide deeper insight into the responses of soil microbes and Pb bioavailability in the presence of nano-ZnO particles.
研究领域:
环境科学
·
土壤微生物学
·
污染控制
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PARTICLE & PARTICLE SYSTEMS CHARACTERIZATION
·
2020
·Vol. 37
·期 3
·1900427
·
IF 3.099
Jia Zhou,Tianliang Li,Yiran Zhang,Xiaoyu Xu,Chunlei Zhang,Yuehua Li,Daxiang Cui,Yingsheng Cheng
Magnetic resonance imaging (MRI) contrast agents are broadly employed for better clinical trials in MR imaging. Magnevist solution (Gd-DTPA), a clinical MRI contrast agent, possesses inherent shortcomings like poor r 1 relaxation, short half-time, nephrotoxicity, etc. To overcome these problems, Gd-DTPA-grafted protein assemblies (Gd-P-ABs) loading with anticancer drug cisplatin and photosentizer IR-780 are constructed via chelation of Gd 3+ . Gd-P-ABs exhibit dual MR/fluorescence (FL) imaging–guided chemo/photothermal therapy. Interestingly, Gd-P-ABs behave as aggregation-enhanced magnetic resonance imaging with an extremely high r 1 value of 26.391 s −1 m m −1 , which is about 5.5-fold larger than Gd-DTPA (≈4.8 s −1 m m −1 ). Consequently, better MRI performance is presented with the same concentration of Gd ions. When exposed to acidic tumor microenvironment and light irradiation, Gd-P-ABs show significant drug release capacity. Good cell killing ability in vitro is also determined due to effective folate-targeting ability and high photo–heat conversion. In vivo MR/FL imaging results reveal that Gd-P-ABs possess high-sensitivity tumor-targeting imaging and long tumor retention, which are attributed to the folate-targeting ability and small size effect. Combined chemo/photothermal therapy in vivo demonstrates that the tumor can be eventually ablated. Altogether, the Gd-P-ABs possess great potential for clinical imaging-guided tumor therapy.
研究领域:
放射学
·
分子影像
·
癌症治疗
·
纳米医学
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
BIOMATERIALS
·
2024
·
IF 12.8
Jinbin Pan,Jiaojiao Wang,Yujie Zhao,Bing Han,Gang Shu,Min Ma,Xu Wang,Xi Wei,Wenjing Hou,Shao-Kai Sun
Anastomotic leaks are among the most dreaded complications following gastrointestinal (GI) surgery, and contrast-enhanced X-ray gastroenterography is considered the preferred initial diagnostic method for GI leaks. However, from fundamental research to clinical practice, the only oral iodinated contrast agents currently available for GI leaks detection are facing several challenges, including low sensitivity, iodine allergy, and contraindications in patients with thyroid diseases. Herein, we propose a cinematic contrast-enhanced X-ray gastroenterography for the real-time detection of GI leaks with an iodine-free bismuth chelate (Bi-DTPA) for the first time. The Bi-DTPA, synthesized through a straightforward one-pot method, offers distinct advantages such as no need for purification, a nearly 100 % yield, large-scale production capability, and good biocompatibility. The remarkable X-ray attenuation properties of Bi-DTPA enable real-time dynamic visualization of whole GI tract under both X-ray gastroenterography and computed tomography (CT) imaging. More importantly, the leaky site and severity can be both clearly displayed during Bi-DTPA-enhanced gastroenterography in a rat model with esophageal leakage. The proposed movie-like Bi-DTPA-enhanced X-ray imaging approach presents a promising alternative to traditional GI radiography based on iodinated molecules. It demonstrates significant potential in addressing concerns related to iodine-associated adverse effects and offers an alternative method for visually detecting gastrointestinal leaks.
研究领域:
医学影像学
·
胃肠病学
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
APPLIED CLAY SCIENCE
·
2023
·
IF 5.6
Kun Jiang,Aihua Xiang,Kun Liu,Qian Peng
Pb and Zn pollution for soil are becoming more and more serious, strengthening the treatment of contaminated soils is conducive to the sustainable development of soil. In this study, the humus-like substances modified montmorillonite (HLS-MMT) were synthesized to remediate the Pb and Zn-contaminated soils. The application of HLS-MMT in simulated and actual contaminated soil were carried, and the long-term stability of HLS-MMT was evaluated. The results showed that the mobility, bioavailability and leachability of heavy metals (HMs) significantly decreased after remediating by HLS-MMT. Compared with MMT, the TCLP leaching ratio ( TLr ) of Pb and Zn reduced by 9.27–13.96% and 10.3–13.18%, while the DTPA effectiveness ( DEr ) Pb and Zn reduced by 9.11% and 8.76% in simulated soil, respectively. Moreover, the aging experiments proved that HLS-MMT possessed a stability to resistance the freeze-thaw cycles, wet and dry alternation, and acid rain leaching. This article provides some theoretical basis for the soil remediation .
研究领域:
环境科学
·
土壤学
·
污染控制
✓ 已验证
产品关联
DOI
高影响因子
Advanced Healthcare Materials
·
2024
·
IF 10
Jiaojiao Wang,Dianxun Fu,Cong Tang,Gang Shu,Xuejun Zhang,Xuening Zhang,Jinbin Pan,Shao-Kai Sun
Digital subtraction angiography (DSA) is considered the “gold standard” for the diagnosis of vascular diseases. However, the contrast agents used in DSA are limited to iodine-based small molecules, which are unsuitable for patients with contraindications. Here, we propose iodine-free DSA utilizing a bismuth chelate, Bi-DTPA Dimeglumine, for vascular visualization for the first time. Bi-DTPA Dimeglumine possesses a simple synthesis process without the need for purification, large-scale production ability (over 200 g in the lab), superior X-ray imaging capability, renal clearance capacity, and good biocompatibility. Bi-DTPA-enhanced DSA can clearly display the arteries of the rabbit's head and lower limbs, with a minimum vascular resolution of 0.5 mm. The displayed integrity of terminal vessels by Bi-DTPA-enhanced DSA is superior to that of iopromide-enhanced DSA. In a rabbit model of thrombotic disease, Bi-DTPA Dimeglumine-enhanced DSA enables the detection of embolism and subsequent reevaluation of vascular conditions after recanalization therapy. Our proposed iodine-free DSA provides a promising and universal approach for diagnosing vascular diseases. This article is protected by copyright. All rights reserved
研究领域:
医学影像
·
放射学
·
血管医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Nanomaterials
·
2016
·
IF 1.758
Zhu Fei-Peng,Chen Guo-Tao,Wang Shou-Ju,Liu Ying,Tang Yu-Xia,Tian Ying,Wang Jian-Dong,Wang Chun-Yan,Wang Xin,Sun Jing,Teng Zhao-Gang,Lu Guang-Ming
Dual-modal imaging by combining magnetic resonance (MR) and near-infrared (NIR) fluorescence can integrate the advantages of high-resolution anatomical imaging with high sensitivity in vivo fluorescent imaging, which is expected to play a significant role in biomedical researches. Here we report a dual-modality imaging probe (NIR/MR-MSNs) fabricated by conjugating NIR fluorescent heptamethine dyes (IR-808) and MR contrast agents (Gd-DTPA) within highly aminated mesoporous silica nanoparticles (MSNs-NH2). The dual-modality imaging probes NIR/MR-MSNs possess a size of ca. 120 nm. The NIR/MR-MSNs show not only near-infrared fluorescence imaging property with an emission peak at 794 nm, but also highly MR relaxivity of 14.54 mM−1 s−1, which is three times more than Gd-DTPA. In vitro experiment reveals high uptake and retention abilities of the nanoprobes, while cell viability assay demonstrates excellent cytocompatibility of the dual-modality imaging probe. After intratumor injection with the NIR/MR-MSNs, MR imaging shows clear anatomical border of the enhanced tumor region while NIR fluorescence exhibits high sensitive tumor detection ability. These intriguing features suggest that this newly developed dual-modality imaging probes have great potential in biomedical imaging.
研究领域:
生物医学工程
·
纳米技术
·
医学成像
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）