JOURNAL OF DISPERSION SCIENCE AND TECHNOLOGY
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2025
·
IF 1.9
Hui Wang,Rui He,Kuan Chang,Jing Xie,Jinhua Li,Guangli Wang,Xiaodong Yan,Jing Wang
Mannose erythritol lipid (MEL-B) lamellar vesicles are promising biocompatible carriers for skincare, but their tendency to form unstable, micron-sized vesicles when used alone limits their application. Herein, we introduce the Gemini surfactant sodium dilauramidoglutamide lysine (DLGL) to overcome this limitation. Highly stable MEL-B-based unilamellar nanovesicles (< 100 nm) are formulated with DLGL. At an optimal DLGL/MEL-B ratio of 0.2, the binary vesicles exhibit excellent stability, showing negligible size variation (< 3 nm) across different storage temperatures and maintaining a zeta potential absolute value consistently above 30 mV. These vesicles significantly enhance the skin permeation of hydrophilic Dendrobium officinale oligosaccharides (DOOS), achieving twice the cumulative permeation compared to the pure DOOS solution at 8 h. Moreover, the formation of ternary vesicles by incorporating lecithin further enhance transdermal delivery.
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PLANT PHYSIOLOGY AND BIOCHEMISTRY
·
2025
·
IF 5.7
Feixiong Zheng,Zhangting Xu,Xiaoji Deng,Yiming Sun,Jaime A. Teixeira da Silva,Xiaoxia Shen,Zhenming Yu
Salt stress severely constrains plant growth and productivity, so understanding the underlying mechanisms of salt stress is crucial for the genetic improvement of plants. Aquaporins (AQPs), which facilitate the transport of water or nutrients, exert an indispensable role in plants' response to salt stress. The AQP family in Dendrobium officinale , a commercially important medicinal and edible orchid, has not been identified or functionally characterized, while an understanding of this plant's tolerance to salinity remains unexplored. In this study, a genome-wide identification of AQP genes in D. officinale was performed using a salt-induced transcriptome. The 28 AQP members were mined and classified into four subfamilies (NIPs, PIPs, SIPs, and TIPs), and their physicochemical and bioinformatic properties were analyzed. Overexpression of DoTIP1-1 in D. officinale significantly enhanced salt tolerance via the accumulation of proline and water-soluble polysaccharides that improved water retention. Furthermore, salt tolerance was fortified by the improved activity of antioxidant enzymes (peroxidase and superoxide dismutase), allowing reactive oxygen species to be scavenged, and augmenting photosynthetic efficiency due to the upregulated expression of stress-responsive genes. This study provides the first comprehensive analysis of the AQP family in D. officinale and demonstrates that DoTIP1-1 is a key genetic determinant for enhancing salt tolerance, offering significant potential for the genetic improvement of this plant to resist abiotic stress.
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CHEMISTRY & BIODIVERSITY
·
2026
·Vol. 23
·期 6
·e71396
·
IF 2.9
Huiqiong Huang,Xun Zhou,Xin Liu,Fuchun Ji,Tang Xu,Yingying Zhao,Qingwen Deng,Xiaofang Yang,Xiaodong Wen
Efficient sample pretreatment is a critical step for the subsequent accurate analysis of target analytes. Herein, a novel dual-function deep eutectic solvent (DES) was synthesized for integrated extraction of flavonoids and digestion of Dendrobium officinale Kimura et Migo ( D. officinale ), to enable dual-mode detection. The one-step pretreatment (integrated extraction and digestion) was not only completed within a mere 50 s but also demonstrated superior performance: it achieved a flavonoid extraction efficiency 1.6−5.6 times that of alcoholic extraction, under milder conditions than traditional digestion methods, while yielding trace element results consistent with microwave digestion-inductively coupled plasma optical emission spectrometry (ICP-OES). Various characterization results show the successful preparation of novel DES, and its mechanism for flavonoid extraction was elucidated through density functional theory calculations. Furthermore, the method's greenness and extraction capacity were rigorously evaluated. Overall, this developed one-step DES pretreatment strategy provided a green, simple, and efficient method for the flavonoid extraction and digestion of D. officinale . Graphical This study pioneers a one-step pretreatment method using a novel dual-functional DES that achieves simultaneous flavonoid extraction and D. officinale digestion in 50 s.
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npj Science of Food
·
2026
·
IF 9.7
Ni Weifeng,Zhou Zhongqi,Mao Shu,Li Zhaolian,Liu Meichen,Zhao Daqing,Sun Liwei,Pan Daian,Liu Shichao,Yin Yiqiu,Wang Siming
Fresh Dendrobium officinale juice (FDOJ) changes color from green to purple after processing; however, the mechanism behind this is unknown. In this study, untargeted and targeted metabolomics were used to analyze pigment-related metabolites. The results showed that the content of delphinidin-type anthocyanin more than doubled in FDOJ after processing. Correlation analysis revealed that major substances in the anthocyanin biosynthesis pathway, including phenylalanine, cinnamic acid, and naringenin chalcone, changed with processing. We speculate that accumulation of anthocyanins during processing occurs through de novo anthocyanin biosynthesis starting from phenylalanine, and the addition of phenylalanine traced that the enhancement of enzyme activity promoted the accumulation of anthocyanins. This potential mechanism for development of the purple color in FDOJ provides theoretical guidance for quality control of FDOJ during processing.
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CARBOHYDRATE POLYMERS
·
2025
·
IF 12.5
Pengfei Dou,Jinglin Wang,Yamin Shu,Fang Cheng,Gang Nie,Li Luo,Zhijing Wu,Ziming Zheng,Yongkang Gai,Junxi Liu,Yan Liu,Yinxing Zhou,Yu Zhang,Xiaoli Lan,Kan Ding,Kaiping Wang
Natural polysaccharides are attracting increasing attention due to their excellent pharmacological properties and low toxicity. However, the clinical translation of these biomacromolecules has been hindered by a lack of pharmacokinetic studies. Polysaccharide labelling presents a promising method for performing pharmacokinetic investigations on polysaccharides. The combination of radioisotopic labelling and high-performance gel permeation chromatography (HPGPC) has not yet been applied to the detection of polysaccharides in biological samples. Here, Dendrobium officinale polysaccharides (DOP) were selected as model polysaccharides. DOP and radiolabelling of DOP ( 99m TC-DTPA-DOP) were prepared and characterized. Then, HPGPC with a radio-HPLC flow detector (radioHPGPC) was used to analyse 99m TC-DTPA-DOP in biological samples for the first time. The detection method was demonstrated to exhibit good linearity (R 2 > 0.997), sensitivity (LLOD <100 nCi, and 62.99 ng/mL for the Conc of DOP), specificity, and recovery rate (>90 %). The blood concentration and distribution in major organs after oral and intravenous administration were studied. The results showed that the bioavailability of DOP was 2.68 %. This method is superior to γ counting or liquid scintillation counting because radioHPGPC could not only eliminate the impact of free 99m Tc and quantity the DOP tracer but also qualitatively analyse the metabolism during absorption and distribution. This study provides detailed pharmacokinetic profiles of DOP and a practical method for labelling and pharmacokinetic studies of other polysaccharides with high molecular weight.
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JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE
·
2020
·Vol. 58
·期 2
·532-540
·
IF 1.946
Xing Li,Miao Yelian,Li Na,Jiang Ling,Chen Jie Yu
One water-soluble polysaccharide (WDOP) and three alkali-soluble polysaccharides (ADOP1, ADOP2 and ADOP3) were successfully obtained from Dendrobium officinale . Molecular structure of the polysaccharides was analyzed, and in vitro lactic acid fermentation of the polysaccharides by lactic acid bacteria (LAB) and bifidobacteria was performed. All the polysaccharides exerted proliferative effect on the LAB and bifidobacteria. ADOP2 was the most effective one, followed by WDOP. This could be attributed to their molecular structure features, such as a high level of total sugar, uronic acid and reducing sugar, an abundance of glucose and mannose, and a low and middle weight-average molecular weight. ADOP2 and WDOP produced more short-chain fatty acids (SCFAs) than ADOP1 and ADOP3 did during lactic acid fermentation. Propionic and acetic acids were the main SCFAs produced. These findings are useful for understanding the structure–activity relationship of D. officinale polysaccharides in lactic acid fermentation, and for developing new functional foods and beverages from D. officinale .
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MOLECULES
·
2022
·Vol. 27
·期 19
·6654
·
IF 4.927
Wang Zhang,Xinjie Liu,Xun Sun,Rongchun Han,Nianjun Yu,Juan Liang,An Zhou
It is generally believed that freshDendrobium officinale(FDO) has more significant pharmacological activity than driedDendrobium officinale(DDO); however, the difference has not been clearly shown. Our study compared their antioxidant properties both in vitro and in vivo, and the molecular weight arrangement and monosaccharide composition of the freshDendrobium officinalepolysaccharides (FDOPs) and the driedDendrobium officinalepolysaccharides (DDOPs) were analyzed by HPLC-GPC and GC-MS. The results showed that the FDO and its polysaccharides had more significant effects on scavenging DPPH, ABTS, and hydroxyl radicals than the DDO. In addition, both the FDO and DDO significantly reduced lipid peroxidation levels and increased the SOD, T-AOC, CAT, and GSH levels in mice with acute liver damage caused by CCl4, while the FDO and its polysaccharides were more effective. Histopathological analysis further verified the protective effect of the Dendrobium polysaccharides on CCl4-induced liver injury. The determination of the polysaccharides revealed that the polysaccharide and mannose contents of the FDO were significantly higher than their dried counterparts, and the homogeneous arrangement of the polysaccharides in the FDO was degraded into three polysaccharide fragments of different molecular weights in the DDO. Overall, our data identified differences in the antioxidant activities of the FDO and DDO, as well as the reasons for these differences.Keywords:Dendrobium officinale;antioxidant activity;polysaccharide;mannose;monosaccharide composition
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Foods
·
2023
·Vol. 12
·期 12
·2370
·
IF 5.2
Li Yao,Xueqin Shi,Hang Chen,Lin Zhang,Lanyan Cen,Lian Li,Yiyi Lv,Shuyi Qiu,Xiangyong Zeng,Chaoyang Wei
Rice, supplemented withDendrobium officinale, was subjected to cofermentation usingSaccharomyces cerevisiaeFBKL2.8022 (Sc) andWickerhamomyces anomalusFBKL2.8023 (Wa). The alcohol content was determined with a biosensor, total sugars with the phenol–sulfuric acid method, reducing sugars with the DNS method, total acids and total phenols with the colorimetric method, and metabolites were analyzed using LC-MS/MS combined with multivariate statistics, while metabolic pathways were constructed using metaboAnalyst 5.0. It was found that the quality of rice wine was higher with the addition ofD. officinale. A total of 127 major active substances, mainly phenols, flavonoids, terpenoids, alkaloids, and phenylpropanoids, were identified. Among them, 26 substances might have been mainly metabolized by the mixed-yeasts fermentation itself, and 10 substances might have originated either fromD. officinaleitself or from microbial metabolism on the newly supplemented substrate. In addition, significant differences in metabolite could be attributed to amino acid metabolic pathways, such as phenylalanine metabolism and alanine, aspartate, and glutamate metabolism. The characteristic microbial metabolism ofD. officinaleproduces metabolites, which are α-dihydroartemisinin, alantolactone, neohesperidin dihydrochalcone, and occidentoside. This study showed that mixed-yeasts cofermentation and fermentation withD. officinaleboth could increase the content of active substances in rice wine and significantly improve the quality of rice wine. The results of this study provide a reference for the mixed fermentation of brewer’s yeast and non-yeast yeasts in rice wine brewing.Keywords:Dendrobium officinale;cofermentation;Saccharomyces cerevisiae;Wickerhamomyces anomalus;metabolites
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PHYTOMEDICINE
·
2024
·
IF 6.7
Ying-Jie Dong,Yi-Piao Zhang,Xiao-Feng Jiang,Zhi-Yi Xie,Bo Li,Ning-Hua Jiang,Su-Hong Chen,Gui-Yuan Lv
s Background The development of gut-liver axis metabolic immune crosstalk is intimately associated with intestinal barrier disorder, intestinal SCFAs-Th17/Treg immunological imbalance, and disorders of the gut microbiota. Prior research has discovered that Dendrobium officinale National Herbal Drink (NHD), a traditional Chinese medicine drink with enhanced immunity, may enhance the immunological response in animals with impaired immune systems brought on by cyclophosphamide by repairing intestinal barrier function and controlling turbulence in the gut microbiota. However, whether NHD can further improve the gut-liver axis metabolic immune crosstalk and its related mechanisms need to be systematically studied. Objectives The purpose of this study is to clarify the function and mechanism of NHD in enhancing the gut-liver axis metabolic immunological crosstalk brought on by excessive alcohol intake. Methods In this work, we set up a mouse model to analyze the metabolic and immunological crosstalk involving the gut-liver axis across 7 weeks of continuous, excessive drinking. At the same time, high and low doses (20,10 mL/kg) of NHD were given by gavage. The effect of NHD on improving the metabolism of gut-liver axis was evaluated by blood lipid, liver lipid deposition, liver function and intestinal pathophysiology. By measuring serum immunological indices, intestinal barrier, and intestinal immune barrier, the impact of NHD on enhancing immune and intestinal barrier function was assessed. Furthermore, immunohistochemistry, immunofluorescence, 16s RNA, Western blot, q-PCR and other methods were used to detect gut microbiota, SCFAs-GPR41/43 pathway, intestinal Th17/Treg immune cells and PPAR-α-NPC1L1/SREBP1 pathway to elucidate the mechanism by which NHD enhances the gut-liver axis' metabolic immune crosstalk. Results Our study demonstrated that NHD has the potential to improve the pathophysiological damage caused by gut-liver axis in model mice. NHD also ameliorated the disorder of lipid metabolism. In addition, it regulated the levels of peripheral blood T cell immunity and serum immune factors. And NHD can restore intestinal mechanical and immune barrier damage. NHD has a favorable impact on the quantity of beneficial bacteria, including uncultured_bacterium_g__norank_f__muribaculacea and uncultured_bacterium_g__Turicibacter . Additionally, it raised the model mice's levels of SCFAs (n-butyric acid, isovaleric acid, etc.). This resulted in the promotion of intestinal GPR41/43-ERK1/2 expression and the reshaping of intestinal CD4 + T cell Th17/Treg homeostasis. As a consequence, colon IL-22 and IL-10 levels increased, while colon IL-17A levels decreased. Lastly, NHD raised the amount of intestinal IAP/LPS, regulated the development of PPAR-α-NPC1L1/SREBP1 pathway in gut-liver axis, and improve lipid metabolism disorder. Conclusions Our study found that NHD can improve the gut-liver axis metabolic immune crosstalk in model mice caused by excessive drinking. The mechanism might be connected to how NHD controls gut microbiota disorders in model mice, the activation of intestinal SCFAs-GPR41/43 pathway, the remodeling of Th17/Treg immune homeostasis of intestinal CD4 + T cells, the improvement of IAP/LPS abnormality, and further mediating the PPAR-α-NPC1L1/SREBP1 pathway of lipid metabolism in gut-liver axis.
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Advances in Medical Sciences
·
2024
·
IF 2.5
Bijun Zeng,Yining Yan,Yujin Zhang,Chang Wang,Wenting Huang,Xinyi Zhong,Zi Chen,Mengzhou Xie,Zhibo Yang
Purpose Psoriasis is a skin disease characterized by excessive proliferation, inflammation and oxidative stress in keratinocytes. The present study aimed to investigate the therapeutic effects of Dendrobium officinale polysaccharide (DOP) on keratinocyte psoriasis-like models. Methods The HaCaT keratinocyte inflammation models were induced by interleukin (IL)-22 or lipopolysaccharide (LPS), respectively, and oxidative stress damage within cells was elicited by H 2 O 2 and treated using DOP. CCK-8 and EdU were carried out to detect cell proliferation. ELISA, qRT-PCR, and Western blot were conducted to measure the expression of pro-inflammatory cytokines IL17A, IL-23, IL1β, tumor necrosis factor alpha (TNF-α), and IL-6. Reactive oxygen species (ROS) level in keratinocytes was detected by flow cytometry. Cell proliferation-associated proteins (PCNA, Ki67, Cyclin D1) and pathway proteins (p-AKT and AKT), and oxidative stress marker proteins (Nrf-2, CAT, SOD1) were detected by Western blot. Result DOP did not affect the proliferation of normal keratinocytes, but DOP was able to inhibit the proliferative activity of IL-22-induced overproliferating keratinocytes and suppress the expression of proliferation-related factors PCNA, Ki67, and Cyclin D1 as well as the proliferation pathway p-AKT. In addition, DOP treatment was able to inhibit IL-22 and LPS-induced inflammation and H 2 O 2 -induced oxidative stress, including the expression of IL17A, IL-23, IL1β, TNF-α, IL-6, and IL1β, as well as the expression levels of intracellular ROS levels and cellular oxidative stress-related indicators SOD, MDA, CAT, Nrf-2 and SOD1. Conclusion DOP inhibits keratinocyte hyperproliferation, inflammation and oxidative stress to improve the keratinocyte psoriasis-like state.
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BMC PLANT BIOLOGY
·
2025
·Vol. 25
·期 1
·1-14
·
IF 4.8
Chen Jing,Li Shoujie,Si Can,Liang Hanzhi,Duan Jun,He Chunmei
Background Abscisic acid (ABA) plays a crucial role in plant growth, development, and stress responses. The pyrabactin resistance-like (PYL) ABA receptor proteins, which are key components of ABA signal recognition and transmission. Dendrobium officinale is a precious traditional Chinese medicinal plant with known drought resistance. Nevertheless, the PYL genes of D. officinale, along with other orchid plants, remains unanalyzed.Results In this study, the PYL family was identified from D. officinale, consisting of 11 PYL proteins. A phylogenetic analysis showed that these DoPYL proteins were classified into three subfamilies. All the DoPYL proteins featured both Motif 1 and Motif 2. Notably, the members of subfamily I and III were intron-less, while those of subfamily II consistently displayed 2 introns. Analysis of expression patterns revealed that these DoPYL genes exhibit diverse spatiotemporal expression profiles in different organs. DoPYL1, DoPYL2, DoPYL4, DoPYL8 DoPYL9 and DoPYL10 followed a decreasing trend as protocorm-like bodies (PLBs) developed into plantlets. Under ABA and mannitol treatments, DoPYL1, DoPYL2, DoPYL4, DoPYL5, DoPYL6 and DoPYL10 exhibited significant down-regulation, while the expression of other genes remained unchanged. Following cold and salinity treatment, there was a notable up-regulation in the expression of the majority of DoPYL genes. PEG-simulated drought stress treatment led to the down-regulation of DoPYL genes. Furthermore, the DoPYL1 protein was localized in the cell membrane, cytoplasm and nucleus. During Yeast two-hybrid Library screening, we identified 40 potential interacting proteins of DoPYL1. Yeast two-hybrid verification confirmed that DoPYL1 interacted with DoRpb3, DoUbox, DoGrx, and DoATPsyn proteins.Conclusion Our results not only contribute to an understanding of the potential role of PYL proteins in growth, development and stress responses in Orchidaceae plants, but also provide additional theoretical guidance for breeding new varieties of D. officinale with resistance to abiotic stresses.
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Polymers
·
2025
·Vol. 17
·期 6
·727
·
IF 4.9
Yanlei Yu,Honggang Wang,Xiaoshu Jin,Wenjing Huang,Yunjie Zhao,Ningning Wang,Dongze Lu,Bin Wei,Hong Wang
Polysaccharides derived fromDendrobium officinalehave been demonstrated to exhibit metabolic regulatory properties. However, the correlation between their structure and function, particularly their mechanism of action through gut microbiota, remains underexplored. This study systematically elucidates the structural characteristics of Dendrobium officinale polysaccharide (DOP) from the Guizhou (GZ) and Zhejiang (ZJ) provinces of China using nuclear magnetic resonance (NMR) and a series of chromatographic analyses, revealing their unique molecular features. Additionally, the metabolic regulatory activities were assessed through α-glucosidase inhibitory assay and in vitro intestinal flora activity assay. The findings include the following: (1) both DOP-GZ and DOP-ZJ predominantly consist of glycosidic linkages of β-1,4-Manpand β-1,4-Glcp; (2) zhe monosaccharide composition ratios of mannose to glucose are 2.51:1 for DOP-GZ and 2.66:1 for DOP-ZJ, with molecular weights of 356 kDa and 544 kDa, respectively, indicating significant structural differences between DOPs from different sources; (3) treatment with DOP-GZ and DOP-ZJ led to alterations in the α-diversity indices andFirmicutes-to-Bacteroidotaratios; (4) more importantly, DOP-GZ and DOP-ZJ significantly increase the abundance of beneficial bacteria (e.g., g_Proteobacteria_unclassified) while suppressing the growth of pathogenic bacteria (e.g., f_Enterobacteriaceae_unclassified), with statistically significant results. These findings not only uncover a novel mechanism by which DOPs regulate metabolism through gut microbiota but also provide a crucial theoretical basis for the application of DOPs in functional foods and pharmaceutical development.
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