Journal of Biomaterials and Tissue Engineering
·
2017
·Vol. 7
·期 2
·162-169
·
IF 1.383
Guo Zhenzhao,Ma Min,Huang Xun,Li Hong,Zhou Changren
In the design of proper scaffolds for tissue engineering, the bulk material properties and scaffold architecture are fundamental because the scaffolds have been shown to have an essential role in the induction of cell adhesion and mediation of cellular behavior. It is popularly accepted
that the surface topography (shape, size, and surface texture) is one of the most vital cues that influences cellular reactions in vivo and cell-material interactions in vitro. The objective of this study was to better understand the impact of topography on MC3T3-E1 pre-osteoblasts'
response to the co-culture of electrospun fibrous scaffolds. Three kinds of biomimetic scaffolds with typical average fiber diameters of 0.347 μm, 0.947 μm, and 6.48 μ were fabricated by adjusting proper electrospinning conditions. Cell morphology, proliferation,
ALP activity, and gene expression of MC3T3-E1 cells cultured on the scaffolds were studied. Cells cultured on 0.35 m scaffolds showed a higher projected area and upregulation of osteogenic phenotype gene, such as Runx2, Col I, ALP, and OCN. For cells cultured on 6.5 μm scaffolds,
a higher proliferation rate and aspect ratio were noted. Our results showed that topographical cues designed into scaffolds can regulate proliferation and differentiation of osteoblastic cells.
研究领域:
组织工程
·
生物材料科学
·
细胞生物学
✓ 已验证
DOI
高影响因子
Journal of Biomedical Nanotechnology
·
2019
·Vol. 15
·期 12
·2351-2362
·
IF 0
Xu Yingjie,Wu Xin,Wang Shuyi,Yang Changzhou,Li Ying,Cao Yi
Hydrogels have been widely used to mimic the biochemical and mechanical environments of native extracellular matrices for cell culture and tissue engineering. Among them, self-assembling peptide hydrogels are of special interest thanks to their great biocompatibility, designability
and convenient preparation procedures. In pioneering studies, self-assembling peptide hydrogels have been used for the culture of bone marrow cells. However, the low mechanical stability of peptide hydrogels seems to be a drawback for these applications, as bone marrow cells prefer hard substrates
for osteogenic differentiation. In this work, we explored the use of hydroxyapatite (HAP)-peptide hybrid hydrogels for three-dimensional (3D) culture and differentiation of osteogenic MC3T3-E1 cells. We used HAP nanoparticles as crosslinkers to increase the mechanical stability of peptide
hydrogels. Meanwhile, HAP provided unique chemical cues to promote the differentiation of osteoblasts. A phosphate group was introduced to the self-assembling peptide so that the peptide fibers could bind to HAP nanoparticles specifically and strongly. Rheological characterization indicated
that the hybrid hydrogels were mechanically more stable than the hydrogels containing only peptides and can be used for long term cell culture. Moreover, the hydrogels were biocompatible and showed very low cytotoxicity. The favorable mechanical properties of the hybrid hydrogels and the chemical
properties of HAP synergistically supported the differentiation of MC3T3-E1 cells. Based on these characterizations, we believe that these hybrid hydrogels can potentially be used as scaffolds for cartilage and bone regeneration in the future.
研究领域:
生物材料
·
组织工程
·
细胞生物学
·
材料科学
✓ 已验证
DOI
PubMed
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2017
·
IF 3.887
Cuixia Yu,Junjun Zhuang,Lingqing Dong,Kui Cheng,Wenjian Weng
Hierarchical porous bioglass films on the tantalum were designed to enhance osteointegration of metallic implants. The films were prepared by a sol-gel method using P123 as the mesopore template and polystyrene microsphere as the nanopore template. The films with 5.4 nm mesopores and 100 nm nanopores (MBG-100) elicited an obviously elongated morphology of the cultured MC3T3-E1 cells, as a result, a higher alkaline phosphatase level was expressed. It is suggested that the nanopores play an important role in regulating cellular behavior by initial protein adsorption through nanopore curvatures. The mesopores were proven very effective for loading rhBMP-2, and the rhBMP-2 loaded on MBG-100 films showed a better function of enhancing osteogenic differentiation, which is attributed to that the nanopore structure could expedite rhBMP-2 release and provide a microenvironment for intensifying the interaction of rhBMP-2 with the cells. Hence, the cell osteogenic differentiation can be enhanced by hierarchical porous bioglass films through both the porous structure and rhBMP-2 induction.
研究领域:
生物材料
·
细胞生物学
·
组织工程
✓ 已验证
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2016
·
IF 3.902
Kena Ma,Dan Huang,Jing Cai,Xinjie Cai,Lingling Gong,Pin Huang,Yining Wang,Tao Jiang
Metal orthopedic implants still face challenges in some compromised conditions, partly due to bio-inertness. The present study aimed to functionalize metallic implants with organic-inorganic nanocomposite (strontium-containing chitosan/gelatin) coatings through a simple single-step electrophoretic deposition under mild conditions. The surface characterization and in vitro cellular response were studied and compared with chitosan/gelatin (CS/G) coatings. SEM images suggested the inorganic nanoparticles may be encapsulated within or mixed with organic polymers. The XRD patterns showed that strontium carbonate was generated in the coatings. The TEM images revealed strontium-containing nanoparticles were released from the coatings in PBS. The continuous release after the initial burst release ensured the enduring effects of the functionalized surface. The tensile bond strength of the coatings to the substrates increased after the addition of strontium. In vitro cellular study confirmed that strontium-containing coatings supported the proliferation of MC3T3-E1 cells and exhibited excellent ability to enhance the differentiation of such pre-osteoblasts. Therefore, such organic-inorganic nanocomposite coatings are a promising candidate to functionalize orthopedic implant surfaces.
研究领域:
生物材料学
·
医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2017
·
IF 7.504
Xin Zhang,Junyu Chen,Xiang Pei,Jian Wang,Qianbing Wan,Shaokang Jiang,Chao Huang,Xibo Pei
Nanoscale zeolitic imidazolate framework-8 (ZIF-8)-modified titanium (ZIF-8@AHT) can enhance osteogenesis in vitro. In this study, we systematically and quantitatively examined the effects of ZIF-8@AHT on osteogenesis, and investigated its ability to form bone in vivo. First, we coated various quantities of nanoscale ZIF-8 crystals on alkali- and heat-treated titanium (AHT) by controlling the concentration of the synthesis solution. We then characterized the ZIF-8@AHT materials using scanning electron microscopy (SEM), powder X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), and determination of the sessile drop contact angle. To illustrate the combined effects of micro/nanotopography and ZIF-8@AHT composition on bone regeneration, we cultured MC3T3-E1 preosteoblast cells on various titanium substrates in vitro by setting pure titanium (Ti) and AHT as control groups. The ZIF-8@AHTs enhanced cell bioactivity compared with AHT and Ti, as evidenced by increased extracellular matrix (ECM) mineralization, collagen secretion and the upregulated expression of osteogenic genes (Alp, Col1, Opg, and Runx2) and osteogenesis-related proteins (ALP and OPG). ZIF-8@AHT-1/8 exhibited better osteogenic activity compared with the other ZIF-8@AHT groups investigated. We subsequently inserted Ti, AHT, and ZIF-8@AHT-1/8 implants into the healed first molars (M1s) of mice, and found that ZIF-8@AHT-1/8 also promoted osseointegration at the bone–implant interface. These results suggest that ZIF-8@AHT-1/8 has great potential for practical application in implant modification.
研究领域:
生物材料
·
组织工程
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2018
·
IF 5.158
Xueqian Wang,Mengliu Zhang,Dawei Zhang,Xinluan Wang,Huijuan Cao,Qian Zhang,Chunyan Yan
Curculigo orchioides , is a traditional Chinese medicine, is used in strengthening tendons and bones. We evaluated the anti-osteoporosis activity of the crude polysaccharide (CO70) isolated from the rhizomes of C. orchioides in ovariectomized rats. CO70 showed excellent anti-osteoporosis activity comparable to that of 17 β -estradiol. To explore the constituents responsible for the anti-osteoporosis activity of CO70, a novel homogeneous heteropolysaccharide, COP70-3, was isolated and purified from CO70. COP70-3 has a main backbone chain of (1→5)-linked α -L-Ara f , (1,3→5)-linked α -L-Ara f , (1→6)-linked β -D-Gal p , (1→4)-linked β -D-Man p , (1,2→5)-linked α -L-Ara f , (1→3)-linked β -L-Rha p , (1, 3→6)-linked β -D-Man p , (1→3)-linked α -D-Gal p A, (1,3→6)-linked β -D-Gal p and (1→6)-linked α -D-Glc p residues. Furthermore, 1.87 nM COP70-3 obviously promoted the differentiation of MC3T3-E1 cells, while 0.94 and 1.87 nM COP70-3 significantly improved the osteogenic mineralization rate. These data indicate that COP70-3 has favorable anti-osteoporosis activity in vitro .
研究领域:
药理学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Acta Biomaterialia
·
2026
·
IF 10.4
Zhongming Zhao,Liming Chen,Xiuqiang Li,Haoyang Hei,Hong Sun,Yi Gao,Mengmeng Yao,Chaojie Yu,Fanglian Yao,Hong Zhang,Junjie Li
Endoscopic submucosal dissection (ESD), while a gold-standard minimally invasive technique for treating early-stage intestinal cancers, poses significant challenges for postoperative mucosal repair, owing to the risk of complications such as bleeding, perforation, and infection. Existing strategies often fail to provide a bioactive healing microenvironment. This study presents a Resolvin E1 (RvE1)-loaded, mucin-based injectable hydrogel (MHSR) designed to address these limitations. Employing a component-based biomimetic approach, this hydrogel combines thiol-modified mucin (MUC-S) with zwitterionic methacrylated hyaluronic acid (HA-SB-MA) via thiol-ene click chemistry. The MHSR hydrogel demonstrates good injectability, self-healing properties, and adhesion to positively charged inflamed tissues via electrostatic interactions and disulfide bonds. It establishes a strong physical barrier that effectively resists biofouling from proteins, cells, and bacteria. Notably, MHSR orchestrates comprehensive mucosal regeneration by scavenging reactive oxygen species (ROS), modulating inflammatory responses, enhancing angiogenesis, restoring tight junction integrity, normalizing goblet cell function, and regulating gut microbiota. In the rat model of rectal ESD, MHSR significantly accelerated wound closure, reduced inflammation, and promoted functional restoration of the mucosal barrier. This multifunctional hydrogel system constitutes a highly promising biomimetic strategy for promoting mucosal repair and remodeling of the intestinal microenvironment after ESD. Statement of Significance The study reports a mucin-based injectable hydrogel (MHSR) loaded with Resolvin E1 for rectal mucosal repair after endoscopic submucosal dissection. It combines thiol-modified mucin and zwitterionic hyaluronic acid to form an adhesive, self-healing barrier with anti-biofouling properties. The hydrogel scavenges reactive oxygen species, modulates inflammation, promotes angiogenesis, restores mucosal integrity, and regulates gut microbiota. It significantly accelerates wound healing and reduces complications in a rat ESD model, offering a promising strategy for post-operative regenerative therapy.
✓ 已验证
DOI
PubMed
高影响因子
Food & Function
·
2019
·Vol. 11
·期 1
·1165-1175
·
IF 3.241
Xiaoxia Chen,Tianyu Li,Degang Qing,Jun Chen,Qian Zhang,Chunyan Yan
Humulus lupulus is a perennial climbing plant of the subfamily Cannabioideae native to the Northern Hemisphere. The primary use of H. lupulus is in the brewing industry, where it is an essential ingredient for imparting a unique flavor (bitterness and aroma) to beer. The female flowers of H. lupulus are also used in traditional Chinese medicine, but the biologically active ingredients underlying its benefits remain unclear. China is the largest producer and consumer of H. lupulus in Asia. Using the waste from the beer-brewing process of H. lupulus as raw materials, the biologically active polysaccharides can be screened. This is useful for the full utilization of H. lupulus, potentially leading to disease prevention and treatment. In this study, we isolated a homogeneous polysaccharide (HLP50-1) with a molecular weight of 49.13 kDa from female flowers of H. lupulus via a DEAE-Cellulose 52 anion exchange column and a Sephadex G-75 gel filtration column. Methylation, GC-MS, and NMR analyses revealed that the HLP50-1 comprised →4)-α-D-Glcp-(1→, →6)-α-D-Manp-(1→, →3)-α-L-Rhap-(1→, β-D-Glcp-(1→, α-L-Araf-(1→, →4,6)-2-OAc-β-D-Galp-(1→, β-D-Galp-(1→, →3,6)-β-D-Glcp-(1→, →2,3,4)-α-D-Xylp-(1→, →6)-α-D-Glcp-(1→, →3)-α-D-Galp-(1→, →4)-α-D-Galp-(1→. Advanced structural analysis showed that the HLP50-1 contained irregular fragments of different sizes and shapes with a smooth surface. The aggregates appeared be composed of accumulated crystals. Furthermore, the osteogenic activities of the HLP50-1 were evaluated via MC3T3-E1 cells in vitro. The results showed that 0.13 μM HLP50-1 led to outstanding proliferation, differentiation, and mineralization of the MC3T3-E1 cells. Therefore, HLP50-1 has osteogenic effects, and it may be a candidate for the treatment of osteoporosis. It has broad application prospects in functional foods, health-care products, and pharmaceuticals.
研究领域:
药理学
·
分子生物学
·
生物技术
✓ 已验证
DOI
PubMed
高影响因子
Food Bioscience
·
2024
·
IF 4.8
Aonan Xia,Yang Jiang,Yuan-Kun Lee,Dong Liang,Bo Yang,Xiaoming Liu,Jianxin Zhao,Hao Zhang,Wei Chen
Fermented milk has abundant bioactive peptides, and there is a growing interest in seeking osteogenic peptides of dairy origin. This study employed computer-assisted screening to identify potential osteogenic peptides from fermented milk by Lactobacillus helveticus and investigated the effects of these osteogenic peptides on the proliferation, differentiation, and mineralization of MC3T3-E1 cells, along with their underlying mechanisms. Three osteogenic peptides were identified: AF10 (ARHPHPHLSF), AY17 (AAGGPGAPADPGRPTGY), and NE19 (NIPPLTQTPVVVPPFLQPE). Treatment with 0.2 μM of AF10, AY17, and NE19 promoted the proliferation of MC3T3-E1 cells by increasing the proportion of cells in the S phase and G2/M phase. Further molecular docking revealed significant differences in the interaction of the three osteogenic peptides with the critical domain (Asn12-Lys13-Leu14-Thr15-Gln16-Leu17-Gly18) of the epidermal growth factor receptor (EGFR). The three peptides exhibited different degrees of promotion on the differentiation and mineralization of MC3T3-E1 cells, as well as significant differences in the expression of genes and proteins related to ERK1/2, RUNX2, and BMP2. The AF10 peptide was selected for further validation of the effect of the mitogen-activated protein kinase (MAPK) signaling pathway on the proliferation and expression of differentiation and mineralization-associated genes in MC3T3-E1 cells by combining MAPK pathway inhibitors. Molecular dynamics simulation revealed the stability of AF10 when binding to EGFR. These findings provide valuable insights into the screening of osteogenic peptides of various origins and the development of functional dairy products accordingly.
研究领域:
食品科学
·
生物化学
·
分子生物学
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2021
·
IF 6.953
Zhenquan Ye,Tianyu Li,Degang Qing,Yu Sun,Haiyun Chen,Qian Yu,Chunyan Yan
Alhagi pseudalhagi , commonly known as camel thorn, is used as an indigenous medicinal plant in China. The present study was designed to elucidate the structure of a novel polysaccharide, APP90-2, isolated from Alhagi pseudalhagi and evaluate its osteogenic activity. A homogeneous polysaccharide (APP90-2) was obtained from A. pseudalhagi via DEAE-52 and Sephacryl S-100 columns, with a molecular weight of 5.9 kDa. Monosaccharide, GC–MS, and NMR analyses showed that APP90-2 consisted of α - l -Rha p -(1→, →3)- α - l -Ara f -(1→, →5)- α - l -Ara f -(1→, →4)- β - d -Xyl p -(1→, α - d -Glc p -(1→, →3,5)- α - l -Ara f -(1→, →4)- β - d -GlcA p -(1→, →4)-3-OAc- α - d -Glc p -(1→, →3)- α - d -Gal p -(1→, →3)- β - d -GalA p -(1→, →4)- α - d -Gal p -(1→, →6)- α - d -Man p -(1→, →4,6)- β - d -Gal p -(1→, and →3,6)- β - d -Glc p -(1→ with relative molar ratios of 4.1:1.8:6.1:6.7:1.7:1.0:1.5:2.7:2.4:1.1:2.3:2.6:1.4:2.0. Morphological analyses revealed that APP90-2 interacted with Congo-red and had an obvious honeycomb structure. Additionally, APP90-2 significantly promoted proliferation, differentiation, and mineralization of MC3T3-E1 cells, indicating that APP90-2 exhibited pronounced osteogenic activity. Therefore, our findings suggest that A. pseudalhagi may be used as an alternative medicine or health supplement for the prevention and treatment of osteoporosis.
研究领域:
药理学
·
生物化学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
THIN SOLID FILMS
·
2014
·
IF 1.867
Kui Cheng,Xiaozhao Wang,Wenjian Weng,Jun Lin,Huiming Wang
Light-induced cell detachment on the substrate of TiO 2 nanodots has been proved to be a safe and attractive method for cell harvesting in tissue engineering. In order to improve the cell release efficiency, bioinert platinum with excellent charge transport loading in biocompatible graphite was prepared. 20 wt.% Pt/C powder showing excellent electrical, chemical and bioactive properties was incorporated to a TiO 2 system. We investigated the biocompatibility of Pt/C–TiO 2 composite films with different amounts of Pt/C with C/tetrabutyl titanate molar ratio varying from 0 to 0.42. The adhesion and proliferation rates of pre-osteoblastic MC3T3-E1 cells (mouse calvaria-derived) on the films prepared with the highest C/TiO 2 molar ratio are higher than that of the pure TiO 2 and other films, indicating a Pt/C dose-dependent effect. It is shown that the detachment efficiency of MC3T3-E1 cells depends on Pt/C amount and UV illumination time. Moreover, films prepared with C/tetrabutyl titanate molar ratio of 0.42 showed 93% cell detachment ratio within 5 min of UV illumination, while bare TiO 2 films only reached 75%, indicating an improved cell release efficiency by the incorporation of Pt/C. However, an interesting phenomenon was found that longer illumination time showed lower detachment ratios to some extent, which is contrary to our previous work. It is believed that this work presents the improved cell detachment efficiency by the incorporation of Pt/C.
研究领域:
生物材料学
·
组织工程学
·
细胞生物学
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2021
·
IF 6.953
Xueshi Luo,Shuyun Zhang,Binghong Luo,Hong Li
Nanostructure provides a closer structural support approximation to native bone architecture for cells and further regulates cell's behavior, resulting in the formation of functional tissues. In this work, three engineering collagen templates with oriented fiber architectures were fabricated via electrospinning (Es), plastic compression and tensile (PCT), and dynamic shear stress (SS) methods. Under the observation of POM, SEM, AFM and TEM, the PCT-template and SS-template are packed with well-oriented nanofibers with the native collagen architecture of 67 nm D-periodicity, and the mechanical properties conferred to the templates are better than that of the Es-template. When mentioning the cell's behavior, MC3T3-E1 adhered to grow along the alignment of collagen fiber orientation when cultured on the PCT-template and SS-template. The SS-template with nano- and micro-ordered architecture guided cells to stretch their plasma along with the orientation of collagen fiber, produce more aligned Type I collagen fibers and promote significantly higher osteogenic differentiation of MC3T3-E1 than the PCT-template and Es-template. Overall, it is strongly argued the feasibility of hierarchical collagen fiber architectures for bone tissue regeneration.
研究领域:
生物材料学
·
组织工程学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）