查找使用阿拉丁产品的科研文献

Promoting effect of ReS2 microsphere on iridium complex nanosheets ECL and its sensing application for bisphenol A

Jing Wang,Shiyu He,Mingfu Ye,Yongping Dong

The inherent water insolubility of iridium complexes seriously restricts their application in aqueous electrochemiluminescence (ECL) sensing systems. In order to solve this problem, this study innovatively used water-soluble polymer polyvinyl alcohol to encapsulate the iridium complex ([Ir(dFppy) 2 (d(t-Bu)bpy)](PF 6 )) in a hydrogel, and successfully obtained nanosheets of iridium complex (IrNS) with good dispersion. Systematic studies on the behavior of IrNS ECL in aqueous media showed strong and stable cathodic ECL when persulfate (S 2 O 8 2− ) was used as a co-reactant. Notably, the introduction of rhenium disulfide microspheres (ReS 2 MS) can produce significant cathodic ECL enhancement effects by efficiently catalyzing the conversion of S 2 O 8 2− to generate more SO 4 − . Bisphenol A (BPA) can react with S 2 O 8 2− to reduce the ECL intensity, based on which an ECL sensor was constructed for its detection. Under optimal conditions, ECL intensity varied linearly with BPA concentration, ranging from 1.0 × 10 −10 to 1.0 × 10 −5 mol L −1 with a detection limit of 5.5 × 10 −12 mol L −1 . The fabricated sensors with high sensitivity and selectivity provide a new avenue for the application of water-insoluble iridium complexes in ECL sensing.

Multiple amplifications of NIR-I ECL signals of Au6PtAg nanoclusters and applications in fast detections of noradrenaline

Fanfan Guo,Yuanyuan Hu,Huiwen Zhou,Yuanjin Li,Jiadi Li,Guichuan Wei,Jing Long Han,Wenyue Gao,Sisi He,Wenxin Niu,Ling Zhang

NIR-I electrochemiluminescence (ECL) is emerging as highly sensitive detection strategy due to its low background at the ambient conditions. Nonetheless, the applications of current NIR-I ECL nanoprobes are limited to the harsh synthetic/testing condition, high toxicity, low signal, and poor biocompatibility. In this study, glutathione/lipoic acid-protected trimetallic Au 6 PtAg nanoclusters (GSH/LA/Au 6 PtAg NCs) are demonstrated as an efficient nanoprobe with strong ECL signals around 850 nm in the presences of triethylamine. Critically, the alloying of Ag  +  into the bimetallic Au 2.5  Pt NCs altered the electronic structures of Pt(II), valences of Au(0), and the zata potentials of NCs, promoting the ECL intensities at 850 nm by 1.0 time. Additionally, the partly ligand-exchange of GSH by LA further increased the ECL intensities at 850 nm by 2.2 times through accelerating electron transfer processes. Meanwhile, the NIR-I fluorescence ( λ emit  = 910 nm) was enhanced. More importantly, the utilization of GSH/LA/Au 6 PtAg NCs μg −1 is enhanced by 3000 times after embedding into SiO 2 microspheres ( D , 318 nm). Because noradrenalin has substantial inhibition effects to NIR-I ECL of GSH/LA/Au 6 PtAg/SiO 2 , 1.0 nM−0.1 mM noradrenalin was detected with the low limit of detection as 0.04 nM (S/N = 3) selectively. Biomolecules, glucose, ascorbic acid, or uric acid didn't interrupt the detection of noradrenalin. The sensitivity of this ECL method to detect noradrenalin is much higher than those in previous reports, enabling the early diagnosis of neurogenic diseases.

A Lable-free ECL Biosensor for The Detection of Uric Acid Based on Au NRs@TiO2 Nanocomposite

Meng Jiang,Jing-Shuai Chen

A novel ultrasensitive electrochemiluminescence (ECL) biosensor was developed to detect uric acid (UA) based on Au NRs@TiO 2 nanocomposite. The Au NRs@TiO 2 nanocomposite was successfully prepared. The morphologies of Au NRs@TiO 2 nanocomposite were observed by scanning electron microscope (SEM), transmission electron microscopy (TEM). Morphological representation results showed that the nanomaterials were well dispersed and had a uniform surface. The characterization of electrochemiluminescence properties indicated that the Au NRs@TiO 2 possessed good ECL behavior. The Au NRs@TiO 2 nanocomposite was used to establish a highly sensitive electrochemiluminescence biosensor for the uric acid. A highly sensitive and selective electrochemiluminescence biosensor was established successfully which based on the Au NRs@TiO 2 nanocomposite. Under the optimal conditions, the biosensor shown a wide calibration range from 40 nM to 28 μM (R = 0.9985) with detection limit of 15 nM. The proposed electrochemiluminescence biosensor provided selective detection of uric acid range from 40 nM to 28 μM (R = 0.9985) with detection limit of 15 nM. This biosensor exhibited stable, sensitive and reproducible detection of uric acid, and was able to be used in clinical detection.

Toward mechanistic understanding of electrochemiluminescence generation by tris(2,2′-bipyridyl)ruthenium(II) and peroxydisulfate

Ping Zhou,Wenxuan Fu,Lurong Ding,Yajuan Yan,Weiliang Guo,Bin Su

Electrochemiluminescence (ECL) generation by tris(2,2’-bipyridyl)ruthenium (Ru(bpy) 3 2+ ) and S 2 O 8 2− is one of most frequently studied cathodic systems. However, the reaction mechanism is yet to be fully resolved, since the reaction involving highly reactive intermediates occurs at the negative potential limit of aqueous solutions. In this work we used bismuth microwire (BiMW) as the working electrode and ECL microscopy (ECLM) as the imaging tool to study the mechanism of cathodic ECL generation by Ru(bpy) 3 2+ /S 2 O 8 2− . Thanks to its high hydrogen evolution overpotential, BiMW electrode could provide us with a wide negative potential window in aqueous solutions. While ECLM allowed us to determine the thickness of ECL layer (TEL), which was found to vary from ca. 3.4 μm to 3.0 μm with increasing the concentration of S 2 O 8 2− . Given the chemical reduction of S 2 O 8 2− by Ru(bpy) 3 + is essential for extending the concentration profile of SO 4 •− , the slightly changed TEL is likely related to the instability of Ru(bpy) 3 + in aqueous solutions. Finally, no ECL was detected for Ru(bpy) 3 2+ -labelled microbeads, implying that S 2 O 8 2− could not work as the cathodic coreactant like tri- n -propylamine (TPrA) as the anodic one in bead-based immunoassays .

Self-enhanced nanohydrogel electrochemiluminescence biosensor based on CRISPR/Cas12a and gold platinum nanoparticles modification for high-sensitivity detection of Burkholderia pseudomallei

Yuexin Wang,Bo Shen,Nini Luo,Cai Li,Haiping Wu,Yanshuang Wang,Shen Tian,Xuemiao Li,Rui Liu,Xinmin Li,Junman Chen,Wei Cheng,Shijia Ding,Rui Chen,Meifang Xiao,Qianfeng Xia

The simple, rapid, and accurate detection of highly lethal melioidosis is crucial for early clinical diagnosis and improving cure rates. Currently, due to time consumption, low sensitivity and detection rate the existing clinical detection methods cannot satisfy the needs of clinical diagnosis. Herein, a novel self-enhanced porous hydrogel material (Au@PEI-ABEI@Pt) for ultrasensitive ECL strategy of detection B. pseudomallei was report. The novel porous hydrogel material composed of PEI-ABEI porous hydrogel, gold nanoparticles (AuNP) and platinum nanoparticles (PtNP) have large specific surface area and porous structure, which not only fix more ABEI to realize self-enhanced ECL signal amplification, but also facilitate ion diffusion and efficient utilization of catalytic materials, realizing rapid electron transfer and zero-distance catalysis, significantly improving the initial signal of ECL sensor. Moreover, the most attractive aspect is that Au@PEI-ABEI@Pt hydrogels with good biocompatibility can achieve widespread application of CRISPR/Cas12a in solid-phase carriers without affecting the sensitivity, specificity and shearing activity of CRISPR/Cas12a. After coupling with the ECL system and CRISPR/Cas12a signal amplification strategy, the Au@PEI-ABEI@Pt can achieve an ultrasensitive ECL assay of B. pseudomallei with the LOD of 5 CFU mL −1 in complex samples, with high specificity and stability to effectively classify B. pseudomallei and other Gram-negative bacteria. This study shows that the developed porous hydrogel materials not only serve as an excellent ECL signal reporter to significantly improve the detection sensitivity of ECL biosensors, but also provide a new approach for the wide application of CRISPR/Cas systems in solid-phase carriers.

ECL Biosensor for Sensitive Detection of Soybean Agglutinin Based on AuPt@C60 Nanoflowers Enhanced N-(aminobutyl)-N-(ethylisoluminol)

Di Liu,Fangxin Hu,Han Zhang,Cong Zhang,Shihong Chen

A sandwich electrochemiluminescence (ECL) biosensing system for soybean agglutinin (SBA) detection was developed with AuPt@C60 nanoflowers (AuPt@C60 NFs) as a novel co-reactant accelerator to enhance the performance of N-(aminobutyl)-N-(ethylisoluminol) (ABEI). In this strategy, nanoflower-liked AuPt@C60 complex served as a matrix for loading a large sum of ABEI and galactosamine (Gal) by Au-N and Pt-N bonds to achieve the nanocomposite Gal-ABEI-AuPt@C60 NFs. Dissolved oxygen was used as a co-reaction reagent, while AuPt@C60 complex acted as a co-reactant accelerator for dissolved oxygen to enhanced the ECL emission of ABEI. Gal, as an identifying element, was highly loaded by electrodeposited gold nanoparticles (Dp Au) on the electrode to recognize SBA, and further combine with Gal-ABEI-AuPt@C60 NFs to form a sandwich model. As a result, the proposed SBA biosensor exhibited a wide linear range from 1.0 ng·mL−1 to 5.0 μg·mL−1 and a low detection limit of 0.33 ng·mL−1. In addition, the biosensor displayed an excellent selectivity and an acceptable stability and reproducibility. The combination of AuPt@C60 NFs and ABEI provided a new ECL sensing platform without exogenous co-reactant.

Detection of acetylcholinesterase based on ECL resonance energy transfer between luminol and gold nanoparticle decorated covalent organic framework

Xinyi Wang,Jing Wang,Houcheng Ding,Yongping Dong

Covalent organic framework (COF) materials exhibit potential application in electrochemical sensing and attract increasing research interests. In this study, a novel signal-off mode electrogenerated chemiluminescence (ECL) sensor based on gold nanoparticle decorated COF (Au@COF) composite was developed. The oxidation of luminol was promoted at ferrocene (Fc) modified electrode to generate strong anodic ECL signal. The resonance energy transfer (RET) between luminol ECL and Au@COF composite could inhibit ECL emission. The acetylcholinesterase (AChE) aptamer connected Au@COF composite was immobilized onto the electrode through DNA hybridization to construct ECL-RET system. The specific recognition between AChE and its aptamer could release Au@COF composite from the electrode surface to block the ECL-RET process and restore the ECL signal. As a result, an ‘‘off-on’’ type ECL sensor was constructed and used to detect AChE in the range of 0.5 nM-1 μM, with a detection limit of 1.7 × 10 -10 mol/L (3σ). The sensor has high sensitivity and good stability, indicating that COF composite has attractive potential application in ECL sensing field.

A CeO2-matrical enhancing ECL sensing platform based on the Bi2S3-labeled inverted quenching mechanism for PSA detection

Yongbei Zhao,Qi Wang,Jianxiu Li,Hongmin Ma,Yong Zhang,Dan Wu,Bin Du,Qin Wei

In this paper, amidogen graphene (NH2-Gr) and Au nanoparticle (NP) functionalized cerium oxide (CeO2) NPs (NH2-Gr/Au@CeO2) have been synthesized. The prepared NH2-Gr/Au@CeO2 exhibits strong electrochemiluminescence (ECL) activity which could be quenched efficiently by bismuth sulfide (Bi2S3). Based on this principle, a novel sandwich ECL immunosensor was constructed for the detection of prostate specific antigen (PSA). NH2-Gr was used as a loading matrix for CeO2 NPs because of its superior conductivity and a large surface area. Au NPs were used to combine anti-PSA 1 (Ab1) to CeO2via the Au–NH2 covalent bond and meanwhile to enhance the sensitivity of the immunosensor. NH2-Gr/Au@CeO2 was used as the ECL response layer. Ag NP functionalized Bi2S3 was used to label the secondary antibody (Ab2). After a sandwich-type immunoreaction, a remarkable decrease of the ECL signal was observed. The signal-off sensor showed a wide linear range response from 1 pg mL−1 to 10 ng mL−1 with a low detection limit (LOD) of 0.3 pg mL−1. The applicability of the proposed ECL immunosensor was evaluated by detecting PSA in human serum samples.

Label-Free Electrochemiluminescent (ECL) Determination of Mercury (II) Based upon the Cation Exchange Reaction with Cadmium Sulfide Nanowires

Jin-Xin Ma,Yu-Ling Wang,Chen Chen,Jun-Tao Cao,Yan-Ming Liu

Enhancing electrochemiluminescence by modifying Fe3CuO4 and CdS@ZnS: A novel ECL sensor for highly sensitive detection of permethrin

Qian Wu,Li Tian,Xiangyu Shan,Shuning Yang,Huiling Li,Chao Li,Juan Lu

A novel electrochemiluminescence (ECL) sensor was constructed for the detection of permethrin by modifying Fe 3 CuO 4 –Ru(bpy) 3 2+ and GO-CdS@ZnS on the electrode. Fe 3 CuO 4 was used as a carrier to adsorb more luminous reagent Ru(bpy) 3 2+ to promote luminescence. Meanwhile, it also can be used as a co-reaction promoter to amplify the initial signal of Ru(bpy) 3 2+ -tri-n-propylamine (TPrA) system. When GO-CdS@ZnS was introduced into this system, the ECL signal was further enhanced. The porous nature of graphene oxide (GO) was utilized to load a large amount of CdS@ZnS, in which CdS@ZnS acted as the co-reactant to amplify the ECL signal. The amount of permethrin (PMT) increased and the ECL signal decreased. Under the optimum conditions, the ECL response was linearly related to the logarithm of PMT concentration. The developed ECL sensor allowed for sensitive determination of PMT and exhibited a wide linear range from 1.0 × 10 −11  mol L −1 to 1.0 × 10 −7  mol L −1 . The limit of detection was 3.3 × 10 −12  mol L −1  (S/N = 3). It can be used for the detection of PMT in vegetable samples.

Aptamer based electrochemiluminescent determination of bisphenol A by using carboxylated graphitic carbon nitride

Cao Hai-Xia,Wang Li,Pan Chang-Gang,He Yu-Sheng,Liang Guo-Xi

An electrochemiluminescence (ECL) based assay is described for the determination of the endocrine disruptor bisphenol A (BPA). The method is based on the use of carboxylated graphitic carbon nitride (C-g-C 3 N 4 ) carrying an immobilized aptamer against BPA. In the presence of BPA, the ECL signal decreases due to ECL energy transfer from excited-state C-g-C 3 N 4 to the BPA oxidation product. Under the optimal conditions, ECL intensity increases linearly in the 0.1 pM to 1 nM BPA concentration range. The detection limit is as low as 30 fM. The assay has excellent sensitivity, outstanding stability and high selectivity. It was applied to the determination of BPA in spiked water samples. Aptamer modified carboxylated graphitic carbon nitride was synthesized and applied in an electrochemiluminescence-based aptasensor for bisphenol A.

Multicolor Iridium(III) Complexes with Host–Guest Recognition Motifs for Enhanced Electrochemiluminescence and Modular Labeling

Xinrui Yang,Yingying Xu,Xiaojin Huang,Junmeng Hang,Weiliang Guo,Zhihui Dai

Cyclometalated Ir(III) complexes with high electrochemiluminescence (ECL) efficiency and appropriate bioconjugation sites are urgently needed in ECL immunoassays (ECLIA). Herein, we report the synthesis, photophysics, electrochemistry, and ECL of six new Ir(III) complexes bearing naphthyl (nap) or adamantane phenyl (adap) substitutions, four of which emit cyan, green, or red light and display 1.7- to 7.5-fold increases in ECL intensity. In combination with DFT/TDDFT calculations, this enhancement is rationalized to the augmented radiative rate that arises from both the strengthened spin-orbit coupling (SOC) and the increased transition dipole moment. In addition, the adap-based Ir(III) complex shows high binding affinity with β-cyclodextrin (β-CD) due to the strong hydrophobic interaction, which enables us to develop a modular strategy for the labeling of Ir(III) complexes with biomolecules and to use hydrophobic luminophores in the aqueous-phase detection. As demonstrated, a novel ECLIA is built up and exhibits a wide linear range from 1 ng/mL to 10 μg/mL and a detection limit of 72 pg/mL for the determination of C-reactive protein (CRP). These findings provide new insights into the design, synthesis, and bio-labeling of highly emissive Ir(III) complexes and pave the way for the development of novel ECLIA based on host–guest recognition motifs.