SENSORS AND ACTUATORS B-CHEMICAL
·
2022
·
IF 9.221
Min Chen,Yang Li,Rui Han,Qiuji Chen,Lingyun Jiang,Xiliang Luo
Herein, an electrochemical immunosensor capable of detecting targets in human serum with ultralow fouling and high sensitivity was successfully constructed through a click reaction. One designed biotinylated peptide was associated with the other peptide functionalized with biotin and azide groups at its two terminals, to form a branched antifouling peptide through the streptavidin-biotin affinity interaction, and it was attached to the electrode to construct an antifouling interface. Then, the exposed azide group on the branched peptide was used to link the antibody modified with 5′-dibenzocyclooctyne (DBCO), and the immunosensor was thus constructed with the aid of the click reaction between the azide group and the DBCO. The developed antifouling immunosensor demonstrated a wide linear response range for carcinoembryonic antigen (CEA) and a low limit of detection of 40 fg mL −1 , which is much superior to the sensing performance of the immunosensor prepared through the normal EDC/NHS method. The immunosensor was capable of assaying CEA in undiluted serum samples with satisfying accuracy when compared with a clinically approved method. This work offered an effective strategy to develop various biosensors through the conjugation of antifouling peptides with different antibodies.
研究领域:
分析化学
·
生物化学
·
生物传感器
·
电化学
·
癌症研究
✓ 已验证
产品关联
DOI
高影响因子
Surfaces and Interfaces
·
2023
·
IF 6.2
Qianwen Yue,Cai Wang,Ke Zhang,Huiyu Wan,Baoliang Sun,Jingguo Wu,Jingyi Sun,Ying Wang
Thoracolumbar fascia (TLF) plays an important role in the biomechanics of the spine. TLF often causes low back pain (LBP), leading to limb movement disorders and numbness. At present, the clinical diagnosis mainly depends on doctors' clinical experience and magnetic resonance imaging. In order to make the diagnosis more convenient and accurate, we designed a "sandwich" biosensor based on surface-enhanced Raman scattering (SERS) to detect C-reactive protein (CRP) and serum amyloid A (SAA) in fascia. In this study, Ag nanocubes (AgNCs) were used as the substrate, so that the antibodies of CRP and SAA were modified on the substrate surface. Then substrate combined with antigen to form the bottom layer of the sandwich. 4-Mercaptobenzoic acid (4-MBA) and Nairn blue chloride (NBA) were used as signalling molecule probes. Hollow gold nanoparticles (HGNPs) modified with (EDC / NHS) enabled the attachment of antibodies to CRP and SAA to the surface of HGNPs. Eventually, the complex formed by the antigen-antibody reaction reacted with the sandwich substrate after the modification reaction to form the final "sandwich" structure. We successfully applied it to the specific detection of inflammatory markers in fascial tissue of TLF. The results showed that it had high stability and repeatability, which can bring some help for clinical work.
研究领域:
生物传感器
·
医学诊断
·
纳米技术
·
炎症标志物
✓ 已验证
产品关联
DOI
高影响因子
Biomaterials Advances
·
2024
·
IF 5.5
Xiaomei Dai,Meijuan Yuan,Yuxia Yang,Mengbo Dang,Jian Yang,Junli Shi,Dianwei Liu,Mingjun Li,Hang Yao,Wenyong Fei
The tendon-bone interface heals through scar tissue, while the lack of a natural interface gradient structure and collagen fibre alignment leads to the occurrence of retearing. Therefore, the promotion of tendon healing has become the focus of regenerative medicine. The purpose of this study was to develop a gradient COL1/ hydroxyapatite (HAp) biomaterial loaded with human amniotic mesenchymal stem cells (hAMSCs). The performance of common cross-linking agents, Genipin, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/N-hydroxysuccinimide (EDC/NHS), and dual cross-linked materials were compared to select the best cross-linking mechanism to optimize the biological and mechanical properties of the scaffold. The optimal COL1/HAp-loaded with hAMSCs were implanted into the tendon-bone rotator cuff interfaces in rats and the effect on the tendon-bone healing was assessed by micro-CT, histological analysis, and biomechanical properties. The results showed that Genipin and EDC/NHS dual cross-linked COL1/HAp had good biological activity and mechanical properties and promoted the proliferation and differentiation of hAMSCs. Animal experiments showed that the group using a scaffold loaded with hAMSCs had excellent continuity and orientation of collagen fibers, increased fibrocartilage and bone formation, and significantly higher biomechanical functions than the control group at the interface at 12 weeks post operation. This study demonstrated that dual cross-linked gradient COL1/HAp-loaded hAMSCs could promote interface healing, thereby providing a feasible strategy for tendon-bone interface regeneration.
研究领域:
再生医学
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组织工程
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生物材料
·
干细胞生物学
·
骨科
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
·
2018
·
IF 5.089
Liming Ge,Mingjin Zhu,Xinying Li,Yongbin Xu,Xiaonan Ma,Rong Shi,Defu Li,Changdao Mu
It is significant to develop new approaches to incorporate naturally active agents into gelatin-based edible films for effective antioxidant and long-term antibacterial activities. Here the naturally active compound rosmarinic acid (RosA) was firstly conjugated onto gelatin backbone via EDC/NHS chemistry. Then the conjugates were plasticized by glycerol and crosslinked by dialdehyde xanthan gum to fabricate active RosA-gelatin edible films. The results indicate that the RosA-gelatin edible films present good water resistance property, strong mechanical property and excellent ultraviolet light barrier ability. The conjugation of RosA confers gelatin-based edible films with effective antioxidant and antibacterial activities as expected. It is interesting that RosA-gelatin edible films after 3 months of storage still show good antibacterial activity as that of newly prepared ones, demonstrating the long-term antibacterial activity. Overall, the fabricated RosA-gelatin edible films may have promising application in the fields of food and pharmaceutical packaging.
研究领域:
材料科学
·
食品科学
·
药学
·
生物技术
✓ 已验证
产品关联
DOI
高影响因子
Military Medical Research
·
2023
·Vol. 10
·期 1
·1-15
·
IF 21.1
Xue Yao-Ting,Chen Ming-Yu,Cao Jia-Sheng,Wang Lei,Hu Jia-Hao,Li Si-Yang,Shen Ji-Liang,Li Xin-Ge,Zhang Kai-Hang,Hao Shu-Qiang,Juengpanich Sarun,Cheng Si-Bo,Wong Tuck-Whye,Yang Xu-Xu,Li Tie-Feng,Cai Xiu-Jun,Yang Wei
Background Reconstruction of damaged tissues requires both surface hemostasis and tissue bridging. Tissues with damage resulting from physical trauma or surgical treatments may have arbitrary surface topographies, making tissue bridging challenging.Methods This study proposes a tissue adhesive in the form of adhesive cryogel particles (ACPs) made from chitosan, acrylic acid, 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS). The adhesion performance was examined by the 180-degree peel test to a collection of tissues including porcine heart, intestine, liver, muscle, and stomach. Cytotoxicity of ACPs was evaluated by cell proliferation of human normal liver cells (LO2) and human intestinal epithelial cells (Caco-2). The degree of inflammation and biodegradability were examined in dorsal subcutaneous rat models. The ability of ACPs to bridge irregular tissue defects was assessed using porcine heart, liver, and kidney as the ex vivo models. Furthermore, a model of repairing liver rupture in rats and an intestinal anastomosis in rabbits were established to verify the effectiveness, biocompatibility, and applicability in clinical surgery.Results ACPs are applicable to confined and irregular tissue defects, such as deep herringbone grooves in the parenchyma organs and annular sections in the cavernous organs. ACPs formed tough adhesion between tissues [(670.9 ± 50.1) J/m2 for the heart, (607.6 ± 30.0) J/m2 for the intestine, (473.7 ± 37.0) J/m2 for the liver, (186.1 ± 13.3) J/m2 for muscle, and (579.3 ± 32.3) J/m2 for the stomach]. ACPs showed considerable cytocompatibility in vitro study, with a high level of cell viability for 3 d [(98.8 ± 1.2) % for LO2 and (98.3 ± 1.6) % for Caco-2]. It has comparable inflammation repair in a ruptured rat liver (P = 0.58 compared with suture closure), the same with intestinal anastomosis in rabbits (P = 0.40 compared with suture anastomosis). Additionally, ACPs-based intestinal anastomosis (less than 30 s) was remarkably faster than the conventional suturing process (more than 10 min). When ACPs degrade after surgery, the tissues heal across the adhesion interface.Conclusion sACPs are promising as the adhesive for clinical operations and battlefield rescue, with the capability to bridge irregular tissue defects rapidly.
研究领域:
生物医学工程
·
组织工程
·
外科材料
·
伤口愈合
·
生物材料
✓ 已验证
DOI
PubMed
高影响因子
Journal of Materials Chemistry A
·
2018
·Vol. 7
·期 1
·157-164
·
IF 9.931
Danyang Zhao,Yuyang Tian,Xiaofei Jing,Yun Lu,Guangshan Zhu
Bisphenol A (BPA), an endocrine disrupting compound (EDC) that affects the human nervous system, is used worldwide and is difficult to remove from water. PAF-1@cellulose nanofibril (CNF) composite aerogels are prepared through a double-cross-linking method and applied as adsorbents to remove BPA from water. With the successful combination of the high surface area of PAF-1 and the bulk skeleton of CNFs, as well as strong interactions between BPA molecules and the networks, the resulting composite aerogels can remove 77.7% of BPA, dissolved in water, within 10 seconds at an adsorption rate of 532.2 mg g−1 min−1, and their removal efficiency reaches 97.0% after 1 hour of adsorption. The adsorption capacity of PAF-1@CNF composite aerogels towards BPA is estimated to be as high as 1000 mg g−1 according to the Langmuir adsorption model.
研究领域:
环境科学
·
材料科学
·
化学工程
·
水处理
✓ 已验证
产品关联
DOI
高影响因子
POLYMERS FOR ADVANCED TECHNOLOGIES
·
2018
·Vol. 29
·期 12
·3159-3170
·
IF 2.137
Kai Huang,Xiao Quan,Xipeng Li,F. Handan Tezel,Baoan Li
Organic fouling has been a major impediment for the widespread application of nanofiltration membranes because it could degrade the performance and shorten the service life of such membranes. In this study, the NH 2 -functionalized silica nanoparticles (NH 2 –SiO 2 NPs) were grafted on the surface of commercial NF90 membrane to improve the hydrophilicity and antifouling property with the N -(3-dimethylaminopropyl)- N -ethylcarbodiimide hydrochloride coupled with the N -hydroxysuccinimide (EDC/NHS) to activate carboxyl groups on the membrane surface. The surface roughness (Ra) of this modified membrane decreased from value of 47.4 nm of the pristine membrane to 43 nm. The water contact angle of the modified membrane decreased from 48.4° to 15°, indicating the high hydrophilicity of the surface. Moreover, the EDC/NHS membrane showed good stability when it was exposed to the ruinous physical stress (2-min sonication). The optimized grafting conditions, including the SiO 2 concentration (0.5 wt%), the activation time of EDC/NHS (20 min), and the reaction time of NH 2 –SiO 2 NPs with membrane surface (12 h), were obtained by a series of experiments. Under the antifouling test of 36 hours with bovine serum albumin (BSA) and hexadecyltrimethylammonium bromide (CTAB) as foulants, the EDC/NHS membrane lost 42.1% and 49.6% of its initial flux, respectively, which is lower than that of the pristine membrane (52.2% and 68%, respectively). After cleaning with deionized water for 2 hours, the EDC/NHS membrane also showed significant improvement of flux recovery ratio (92% and 87%, respectively) than did the pristine membrane (70.3% and 60%, respectively).
研究领域:
环境工程
·
膜科学
·
水处理
✓ 已验证
产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2021
·
IF 4.821
Yi Zhang,Yuanxiang Huang,Zhenfeng Yue,Huajun Fan,Shaojing Wu
A novel, efficient and specific aptamer-functionalized sorbent was successfully synthesized and applied in the analysis of ultra-trace aflatoxin M1 (AFM1) and its analogues in milk. By using a gold nanoparticle-based colorimetric analysis tool, we could determine that the AFM1-aptamer (M1-aptamer) recognized and bound AFM1 to generate an aptamer-target complex. After analysis and verification, the M1-aptamer on the surface of the carboxyl microspheres were completely modified via EDC/NHs amide formation. The effects of extraction pH, extraction buffer, concentration of Mg 2+ and elution solvents were investigated by single-factor experiments to determine the most suitable analysis conditions. Using these conditions, the extraction performance of the M1-aptamer-functionalized sorbent could be studied and an analytical method for AFM1 and its analogues in milk could be established. The results of adsorption equilibrium as well as adsorption selectivity experiments indicated that the M1-aptamer offered a specific adsorption site to selectively adsorb aflatoxins exhibiting a dihydrofuran oxaphthalene scaffold. The adsorption capacity of the aptamer-functionalized sorbent was 233.1 μg/g. A studied method for the determination AFM1 in milk with low matrix effect demonstrated a good linear correlation in the range of 0 to 2.0 μg/L (R 2 = 0.9982) and high detection sensitivity (detection limits of 7 ng/kg in milk and 21 ng/kg in milk power). Furthermore, excellent spiked recovery and good repeatability were obtained. The method delivered specific, accurate and reproducible results for enriching and detecting ultra-trace AFM1 and its analogues in milk.
研究领域:
分析化学
·
食品科学
·
纳米技术
·
毒理学
✓ 已验证
产品关联
DOI
高影响因子
POLYMER ENGINEERING AND SCIENCE
·
2016
·Vol. 56
·期 12
·1432-1436
·
IF 1.719
Shiping Zhan,Nan Liu,Weijing Wang,Shuhua Chen,Jingchang Wang
To improve the properties of chitosan (CS) and poly(L-lactic acid) (PLLA) and obtain fully biodegradable materials, CS- g -PLLA copolymers were prepared using 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC)/N-hydroxyl succinimide (NHS) as a coupling agent. The copolymers were characterized by Fourier transform infrared analysis (FTIR), 1 H nuclear magnetic resonance ( 1 H NMR), elemental analysis, differential scanning calorimetry (DSC), X-ray diffraction (XRD) and scanning electron microscopy (SEM). The results obtained by FTIR and 1 H NMR showed that CS and PLLA were grafted successfully via an amide bond. DSC and XRD results showed that the thermal stability of CS had been significantly improved by grafting PLLA to the molecular chains of CS and the crystallinity of the CS- g -PLLA copolymers decreased significantly. Elemental analysis showed that the achieved the maximum degree of substitution of PLLA was 60.88%, while the concentration of CS was 2 mg/mL, the PLLA molecular weight was 10,000, and the EDC/NHS ratio was 2:1. Images from SEM demonstrated that the copolymers had a spherical shape and smooth surface. Moreover, the products were well dispersed without any aggregation. POLYM. ENG. SCI., 56:1432–1436, 2016. © 2016 Society of Plastics Engineers
研究领域:
高分子科学
·
材料科学
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
KEY ENGINEERING MATERIALS
·
2018
·
IF 0
Yang Zi Zhao,You Fa Wang
Being one of the three elements of tissue engineering, three-dimensional porous structure scaffold plays an important role in tissue engineering. As it not only prvovide cells for the life, but also serves as a template to guide tissue regeneration and control of organizational structure and other functions. In this study, hyaluronic acid and gelatin are successfully cross-linked by 1-ethyl- (3-dimethylaminopropyl) -carbodiimide hydrochloride (EDC) , and compound β-TCP microspheres to prepare porous hydrogel scaffolds. The microspheres were analyzed by X-ray diffraction (XRD). The scaffolds were characterized by scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR). At the same time, the compressive strength, swelling ratio, degradation of the scaffold were tested. To assess the in vitro cell compatibility of the scaffolds, mouse L929 fibroblasts were seeded onto scaffolds for cell morphology and cell viability studies. The results showed that the pore size of the porous scaffold can be adjusted by changing the ratio of gelatin to hyaluronic acid (HA), increasing the proportion of hyaluronic acid in a certain range, pore size will be significantly increased. With the increase of the proportion of hyaluronic acid in the scaffold, the swelling ratio and the degradation rate also increased. The compressive strength of the scaffold increased with the increase of the proportion of gelatin. The appropriate ratio of β-TCP can promote cell growth and proliferation.
研究领域:
生物材料
·
组织工程
·
生物医学工程
·
材料科学
✓ 已验证
产品关联
DOI
Journal of Materials Chemistry B
·
2019
·Vol. 8
·期 4
·758-766
·
IF 5.047
Dong Han,Baolin Zhang,Chuangang Chong,Cuiping Rong,Jie Tan,Rusen Yang
Effective attachment of magnetic nanoparticles to neuronal membranes has far-reaching significance in activating ion channels and treating neurodegenerative diseases. Superparamagnetic iron oxide nanoparticles (SPIONs) synthesized by the polyol pyrolysis method have the advantages of rich surface functional groups, excellent magnetic properties, controllable particle size and water dispersibility. We propose that perfusion of biotin into the targeted brain area should be initially performed because it tends to be adsorbed by cell membranes, followed by injection of streptavidin (SA)-modified SPIONs into the same area of the brain. By means of the strong binding force between SA and biotin, the SPIONs may subsequently adhere to the cell surfaces in the brain area. In this work, fluorescein isothiocyanate-streptavidin (FITC-SA) was modified on the surface of polyethylene imine (PEI)-SPIONs by the EDC–NHS method and stereotaxically injected into the biotin-supplemented substantia nigra of mice. The combination of fluorescence detection with transmission electron microscopy (TEM) confirmed that FITC-SA/PEI-SPIONs adhered to neuronal membranes in the substantia nigra of mice 24 h after injection. The results show that our strategy can promote the attachment of SPIONs to neuronal membranes.
研究领域:
神经科学
·
纳米技术
·
生物医学工程
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD AND AGRICULTURAL IMMUNOLOGY
·
2014
·
IF 0.984
Xinlin Wei,Yaru Zhao,Baoqin Wang,Yuanfeng Wang
Two kinds of new immunogens and coating antigens had been designed and synthesised by methods of lively ester (DCC) and carbodiimide (EDC), respectively. Preliminary immunosorbent assays indicated that the two target conjugates presented good inhibition ratio against the haptens of cypermethrin in vivo. The results showed that the best efficient titres of anti-cypermethrin rabbit serum in methods of DCC and EDC were 1:32000 and 1:64000, the IC50 of them were 18.907µg/L and 16.877µg/L against the haptens of cypermethrin, respectively. The data indicated that the anti-cypermethrin antibodies in method of EDC had higher sensitivity and higher selectivity than the anti-cypermethrin antibodies in method of DCC. Thus, we can synthesise antigens and anti-bodies with method of EDC. The antibodies of high quality can be effectively used in the field of the rapid detection of pyrethroid pesticide residues in the research of the reagent strips.
研究领域:
分析化学
·
环境科学
·
毒理学
·
农业科学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）