BIOELECTROCHEMISTRY
·
2025
·
IF 4.5
Zhide Zhou,Haimei Li,Xiaohua Deng,Yuxuan Shi,Jiahang Huang,Yu Zhou,Xiaohong Tan,Jintao Liang,Guiyin Li
Cardiovascular disease (CVD) is the leading cause of death worldwide, particularly in the developing countries. Low-density lipoprotein (LDL), a critical biomarker for the early detection and intervention of CVD, provides valuable diagnostic and prognostic information for CVD. Herein, we developed a dual-signal electrochemical aptasensor for LDL detection based on a peroxidase-like nanozyme composed of reduced graphene oxide@molybdenum disulfide-ferrocenecarboxylic acid (rGO@MoS 2 -Fc). In the process of electrochemical testing, rGO@MoS 2 -Fc achieved the synergistic amplification of electrochemical signals both an enhanced Fc oxidation current detected by differential pulse voltammetry (DPV), and an increased hydrogen peroxide (H 2 O 2 ) decomposition current via amperometric current-time (i-t) analysis. The DPV signal, corresponding to Fc oxidation, exhibited a logarithmic linear response over a broad LDL concentration ranged from 0.001 to 100.0 μg/mL, with a limit of detection (LOD) as low as 0.91 ng/mL. Meanwhile, the i-t signal, arising from H 2 O 2 decomposition, displayed a linear range of 1.0 to 80.0 μg/mL and an LOD of 0.80 μg/mL. This dual-signal strategy exhibited good selectivity, reproducibility, stability and self-calibration capability. Analysis of human serum samples showed that the proposed strategy is expected to be a powerful method for early-stage CVD diagnosis and clinical management.
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高影响因子
BIOELECTROCHEMISTRY
·
2024
·
IF 4.8
Guiyin Li,Bo Wang,Shengnan Li,Xinhao Li,Ruijie Yan,Xiaohong Tan,Jintao Liang,Zhide Zhou
Golgi protein 73 (GP73) is a novel tumor marker in the early diagnosis and prognosis of hepatocellular carcinoma (HCC). Herein, a competitive electrochemical aptasensor for detecting GP73 was constructed using reduced graphene oxide-ferrocene-polyaniline nanocomposite (rGO-Fc-PANi) as the biosensing platform. The rGO-Fc-PANi had larger specific surface area, excellent conductivity and outstanding electroactive performance, which served as nanocarrier for GP73 aptamer (GP73 Apt ) binding and as redox nanoprobe for record electrical signal. Then, a complementary chain (cDNA) was fixed to the electrode by hybridization with GP73 Apt . When GP73 was present, a competitive process happened among cDNA, GP73 Apt and GP73, formed the GP73-GP73 Apt stable chemical structure and made cDNA detach from the sensing electrode, resulting in enhancement of electrical signal. The difference in the corresponding peak current before and after the competition can be used to indicate the quantitative of GP73. Under optimal conditions, the DPV current response showed a good log-linear relationship with GP73 concentrations (0.001 ∼ 100.0 ng/mL) with a detection limit of 0.15 pg/mL (S/N = 3). It was successfully used for GP73 detection in human serum with RSDs ranging from 1.08 % to 6.96 %. Therefore, the aptasensor could provide an innovative technology platform and hold a great potential in clinical application.
研究领域:
生物医学工程
·
肿瘤学
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PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2020
·
IF 3.99
Ang Li,Mengqi Zhu,Pengfei Hao,Haihao Wu,Jing Gong,Kun Wang,Weichao Yu,Yaorong Feng
To inhibit wall sticking during gathering of high water cut crude oil, based on the deposition characteristics of the complexes by reaction of phytic acid (PA) with ferric chloride (FC), a simple and environmentally friendly method for carbon steel plate modification was adopted in this study. The effects of reaction time, FC concentration, and deposition times on surface wettability and morphology were investigated. It appeared in the results that the wettability of the surface no longer changed after 1 min of reaction. After two times of deposition, the carbon steel exhibited superhydrophilicity in air and superoleophobicity under water, and more pronounced nano-structures were observed. Comparing with similar works, no electroplating, heating and other devices are required during the entire coating process . Moreover, for the sake of clearly explaining the mechanism of wettability conversion during PA-FC modification, the theory of surface energy and adhesion work were used to quantitatively calculate the adhesion strength of oil droplet on solid surfaces . It was found that the hydrophilization and underwater oleophobicization of materials were essentially a process of increasing the polar component of surface energies, and the opposite adhesion state of oil in air and under water was demonstrated theoretically. Finally, the anti-sticking ability at low temperatures of treated carbon steel was evaluated experimentally. The PA-FC coating showed good oil gel prevention performance below the pour point of crude oil. In conclusion, the wall sticking prevention method proposed in our work provides another way for flow assurance of the transportation of high water cut crude oil.
研究领域:
材料科学
·
石油工程
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高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Jintao Liang,Qing Huang,Liang Wu,Xiaohang Shi,Kaiteng Yan,Fei Guo,Zhide Zhou,Guiyin Li
Glucose (Glu) is an important source of energy and metabolic intermediate for animal and plant cells. In this study, a high-performance biosensor for specific detection of Glu was constructed by combining a light-addressable potentiometric biosensor (LAPS) and a specific sensitive unit. The specific sensitive unit was adopted a silicon-based capacitive structure, and the glucose oxidase/reduced graphene oxide -chitosan-ferrooxidase/gold nanoparticles (GOX/RGO-CS-Fc/AuNPs) was modified onto the silicon-based working electrode to construct an nanosensing membrane. The interaction between the specific sensitive unit and Glu resulted in the formation of a bilayer potential, and the field effect generated by the bilayer potential caused a change in the capacitance of the silicon structure, which resulted in a change in the photocurrent-voltage characteristics of the LAPS. Through the potential shift in the photocurrent-voltage characteristics, the LAPS was capable of detection the concentration of Glu. Under optimal conditions, the potential shift was linearly correlated with the Glu concentration from 0.01 mg/mL to 4.00 mg/mL with R 2 of 0.9821, and the limited detection was 0.001 mg/mL. In addition, the LAPS showed good recovery (97.92 %–105.72 %), admirable specificity and stability in detection Glu in human serum samples.
研究领域:
生物传感器
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纳米技术
·
分析化学
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生物医学工程
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高影响因子
JOURNAL OF ALLOYS AND COMPOUNDS
·
2023
·
IF 6.2
Meng Zhu,Dong-Xing Tan,Xiang-Yu Wang,Qing-Nan Bian,Ben-Shuai Guo,Yan-Min Liu,Gao Deng,Yuan-Yuan Feng
Water electrolysis to produce high-purity hydrogen has been regarded as the most promising and attractive method for renewable energy production. The intrinsic high kinetic barrier of the involved electrode reactions, especially that of the anodic oxygen evolution reaction (OER), however severely hinders the application of the water electrolysis technology for large scale hydrogen production. In this paper, a nano-composite Fe- and Co-based material containing crystalline metal sulfide ((NiFeCo)S) and amorphous metal phosphide ((FeCo)P) was in-situ grown on the surface of foam nickel (NF). The (FC)P-(NFC)S/NF catalyst exhibits ultra-high activities for OER at high current densities in 1.0 M KOH, delivering 500 and 1000 mA cm −2 at ultralow overpotentials of 274 and 280 mV, respectively with a small Tafel slope of 37.8 mV dec −1 . The excellent catalytic activities of (FC)P-(NFC)S/NF far exceed those of the reference single-component (FC)P/NF and (NFC)S/NF catalysts, and also exceed those of most non-noble metal catalysts reported in literature. Besides, the (FC)P-(NFC)S/NF catalyst exhibits high stability with almost no significant activity attenuation after the chronopotentiometric test at both 100 and 500 mA cm −2 for 40 h. The synergistic effects between the metal sulfide and metal phosphide have positive impacts on the OER activity through increasing the surface area, enhancing the reactivity of each active site, improving the electrical conductivity as well as the interface between the crystalline and amorphous phases. The present work provides a new strategy to develop cost-effective and efficient electrocatalysts toward OER and water electrolysis.
研究领域:
材料科学
·
能源科学
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高影响因子
MICROCHEMICAL JOURNAL
·
2023
·
IF 4.8
Jintao Liang,Fei Guo,Jiaying Huang,Xiaohong Tan,Lixian Sun,Zhide Zhou,Guiyin Li
Golgi protein-73(GP73) is one of the most common biomarkers with outstanding sensitivity and specificity in the diagnosis of hepatocellular carcinoma. Herein an ultrasensitive label-free electrochemical aptasensing platform was constructed for GP73 detection based on reduced graphene oxide-ferrocene formic acid-tripterythrine tetroxide nanocomposites (RGO-Fc-Mn 3 O 4 ) and GP73 aptamer (GP73 Apt ). Gold@poly-o-phenylenediamine (Au@PoPD) was electrodeposited on the surface of screen-printed electrode (SPE) as the conductive substrate. RGO-Fc-Mn 3 O 4 was then drop-coated on Au@PoPD/SPE as a signal probe to generate measurable redox peaks due to the electroactivity of Fc in RGO-Fc-Mn 3 O 4 . GP73 Apt was anchored on the surface of RGO-Fc-Mn 3 O 4 NPs/Au@PoPD/SPE to constitute an electrochemical aptasensing biosensor. When GP73 was captured via the specific affinity of GP73 Apt and formed GP73-GP73 Apt complex onto the aptasensing platform, the formation GP73-GP73 Apt complex impeded the electron transfer on the electrode interface, resulting in a decrease in the peak current recorded by differential pulse voltammetry method. Thus, GP73 can be detected by measuring the change of Fc redox peak current in RGO-Fc-Mn 3 O 4 . Log-linear calibration curve for GP73 concentration (0.01–1000 ng/mL) was obtained with good correlation coefficients of 0.99485 and a lower limit of detection of 0.01 ng/mL. The human serum samples were tested by direct detection method, and the relative error and relative standard deviation are 4.21–9.87% and 3.28–9.40%, respectively. All these results demonstrate that the proposed aptasensor would be a promising tool for clinical analysis of GP73 in the diagnosis of hepatocellular carcinoma.
研究领域:
生物医学工程
·
分析化学
·
癌症诊断
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高影响因子
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
·
2026
·
IF 2.9
Jin Xu,Anqi Zhou,Mengjiao Xu,Qiaoshuang Chen,Chenjing Xu,Jinhao Li,Na Li,Zhixin Li,Guodong Wu,Weitao Zhang,Shuhai Xu,Qiaoling Ni,Tao Liu,Chunguang Zheng,Wei Yu,Xi Chen,Lankun Song,Min Xia,Qingcheng Guo,Lusha Ji,Weizhu Qian,Sheng Hou,Jun Li,Ziqiao Sun,Xuekun Wang,Fubo Han,Hao Wang,Jing Li,Dapeng Zhang,Huaizu Guo
Fc-containing GLP-1 therapeutics exhibit complex post-translational modification (PTM) heterogeneity, necessitating advanced analytical methods for quality control (QC) and process analytical technology (PAT). We developed a reverse-phase liquid chromatography (RP-LC) method for the PTM-specific profiling of these biologics. Using dulaglutide (IgG4-Fc) as a model, critical parameters─including mobile-phase additives, acid concentration, and shallow gradients─were optimized to resolve PTM variants (e.g., hydroxylation, N-terminal truncation, disulfide reduction, glycosylation) within 40 min. Mass spectrometry (MS) compatibility was enabled by adopting difluoroacetic acid (DFA) as an alternative ion-pairing reagent to support intact-mass characterization of variants. This enabled the identification of additional PTMs not readily resolved under the initial RP-LC conditions, including site-specific HyK-Gal-Glc O-glycosylation and process-dependent truncations. The method also allowed for the direct quantification of critical impurities and the detection of process-induced variants across biosimilar clones. The method was further demonstrated on the IgG2-subtype GLP-1-Fc-fusion (supaglutide), showing applicability across the two Fc subtypes examined without additional optimization. This robust, MS-compatible RP-LC platform provides a rapid, accurate, and comprehensive (RAC) means of conducting PTM-specific QC for Fc-GLP-1 therapeutics, supporting PAT implementation and accelerating biosimilar and next-generation drug development.
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高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2026
·
IF 12.5
Xiaolei Zhang,Nan Gao,Zong Tan,Dongjun Lv,Zhongmin Liu,Qi Tang,Fei Yang,Xiaoli Wang,Hui Zhao,Xueting Liu,Jinyang Li,Jiahao Wang
ER-Fc-MOF@CF monolith integrates PE capture and Fenton-like oxidation. • Carbonization tunes conductivity, defects, and MOF anchoring on CF. • CF-900 achieves 91.14% PE removal under flow-through conditions. • Captured PE undergoes localized ROS-mediated surface oxidation. • Monolithic design reduces powder catalyst loss and secondary release. Microplastic remediation requires efficient particle capture and interfacial oxidation because dispersed polymers have limited contact with short-lived reactive oxygen species (ROS) in bulk solution. Here, an electroresponsive ferrocene metal–organic framework (ER-Fc-MOF) was grown in situ on thermally engineered foam carbon (CF) to construct ER-Fc-MOF@CF monoliths for polyethylene microplastic capture and subsequent electro-Fenton-like treatment. Carbonization temperature regulated the scaffold architecture, surface chemistry, defect structure, electrical resistivity, and ER-Fc-MOF coverage. Among the investigated samples, CF-900 retained accessible hierarchical porosity while exhibiting the lowest resistivity and densest ER-Fc-MOF coverage. The optimized ER-Fc-MOF@CF-900 achieved 91.14% flow-through removal from a 100 mg·L −1 polyethylene (PE) suspension. Zeta-potential, FTIR, and XPS analyses suggested pH-dependent electrostatic attraction and localized polar interactions involving oxygen-containing groups, while the interconnected monolith facilitated particle interception and retention. The low-resistance CF-900 scaffold supported electron transport and H 2 O 2 activation at ER-Fc-MOF domains. SEM, EDS, FTIR, and XPS showed progressive PE surface oxidation, including roughening, oxygen enrichment, and increased C O, C O, and O–C=O signals, while EPR identified ·OH, ·O 2 − , and 1 O 2 . After 36 h, dissolved organic carbon (DOC) reached 67.21 mg·L −1 in the PE-containing system versus 8.80 mg·L −1 in the PE-free control, supporting PE-associated dissolved organic carbon release. This work establishes a self-supporting MOF–carbon platform coupling PE capture, flow-through interception, and interfacial oxidative transformation. Download: Download high-res image (205KB) Download: Download full-size image
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Analytical Methods
·
2024
·
IF 2.7
Zhiren Zhou,Yuting Tian,Lina Zou,Yanan Liu,Xueqing Zhang,Xiaojing Huang,Huanyu Ren,Zheng Li,Huiru Niu,Hao Liao,Xiaojing Zhang,Hongzhi Pan,Shengzhong Rong,Hongkun Ma
Nuclear matrix protein 22 (NMP22) is one of the most important tumor markers of bladder cancer and is significantly elevated in the urine of bladder cancer patients. Therefore, in this work, a highly sensitive ratiometric electrochemical immunosensor was constructed to detect NMP22 based on ZIF-8@MWCNTs@Chit@Fc@AuNPs composites. ZIF-8 had a large surface area and good adsorption ability. Multi-Walled Carbon Nanotubes (MWCNTs) can optimize the electrical conductivity of ZIF-8, so that the electrode surface of ferrocene (Fc) obtains a stable and strong electrochemical signal. In addition, AuPt-MB provided another strong detection signal methylene blue (MB) while immobilizing the secondary antibody (Ab2) through Au–N and Pt–N bonds. A ratiometric electrochemical sensor was formed based on ZIF-8@MWCNTs@Chit@Fc@AuNPs and AuPt-MB, which showed a great linear connection between IMB/IFc and the logarithmic concentration of NMP22 with a detection limit of 3.33 fg mL−1 (S/N = 3) under optimized specifications in the concentration interval of 0.01 pg mL−1 to 1000 ng mL−1. In addition, the ratiometric immunosensor showed good selectivity and stability.
研究领域:
生物医学工程
·
肿瘤学
·
分析化学
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PubMed
高影响因子
Analytical Methods
·
2021
·Vol. 13
·期 17
·2011-2020
·
IF 2.896
Yi-Xin Sun,Dan-Dan Zhang,Yang Sheng,Defeng Xu,Rong Zhang,Mark Bradley
The β-CD@PEI-Fc chiral interface was prepared based on the supramolecular host–guest interaction between ferrocene (Fc) grafted polyethyleneimine (PEI-Fc) and chiral β-cyclodextrin (β-CD). SEM results show that β-CD@PEI-Fc interface has a regular spatial structure, which can effectively distinguish tryptophan (Trp) enantiomers. Under the optimal conditions, differential pulse voltammetry shows that the peak current ratio (ID/IL) of Trp enantiomers can reach 2.84 at 15 °C. More interestingly, the β-CD@PEI-Fc/GCE exhibited chiral recognition of D-Trp and L-Trp via water contact angle measurements. There was a good linear relationship between the peak current and the concentration of Trp enantiomers in the range from 0.005 mM to 0.10 mM. Finally, the chiral interface can be applied for quick detection of the proportion of isomers in Trp racemic solution, which is very important for chiral recognition in racemic mixture of chiral compounds. Meanwhile, the β-CD@PEI-Fc/GCE showed good stability and reproducibility.
研究领域:
分析化学
·
电化学
·
手性
·
材料科学
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PubMed
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Yihao Li,Fengzhen Jin,Bowen Li,Shun Wu,Yuanqing Wang,Yaohui Wu,Yonghong Wang,Ge Ning
Zearalenone (ZEN) is a mycotoxin that exerts multiple toxic effects on humans through the contamination of grains such as corn, posing a significant threat to food safety and human health. This work proposed a dual-signal ratiometric electrochemical aptasensor utilizing ferrocene-carboxylic acid‑gold nanoparticle‑molybdenum disulfide nanosheets (Fc-Au-MoS 2 ), hybridization chain reaction (HCR), and the cleavage effect of DNAzyme triggered by triple-helix molecular switch (THMS) to detect ZEN. The specific interaction with ZEN triggers the release of the DNAzyme from THMS, which then removes the immobilized primer chain on the electrode surface with the assistance of Pb 2+ , thereby preventing HCR occurrence. This inhibitory mechanism reduces methylene blue (MB) insertion while exposing the active sites of Fc-Au-MoS 2 , generating an opposite signal response to achieve high-precision ratio detection. The aptasensor demonstrated a low detection limit of 29.4 pM, with satisfactory recoveries in corn flour (97.3 %–106.4 %), confirming its potential for monitoring mycotoxin residues in food safety.
研究领域:
分析化学
·
食品安全
·
生物传感器
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纳米材料
·
环境监测
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高影响因子
Acta Pharmaceutica Sinica B
·
2025
·
IF 14.6
Chaoyan Yao,Lei Wang,Weidong Liu,Ning Shi,Ziling Liao,Yuxuan Fu,Jinhao Ouyang,Xuan Lei,Qianping Huang,Siyu Li,Yihua Zhouyang,Pinnan Zhao,Jie Wang,Hongjuan Xu,Wenhu Zhou,Xingjun Jiang,Xiang Gao,Caiping Ren,Longlong Luo
The effective treatment of nasopharyngeal carcinoma (NPC) is challenged by an immunosuppressive tumor microenvironment (TME) and insufficient immune effector cell activation. Herein, we design a synergistic tri-modal therapeutic strategy to overcome these barriers. This platform integrates: (1) a CD109-targeted liposomal doxorubicin (S3-Lip-DOX) for precise chemotherapy and induction of immunogenic cell death (ICD); (2) non-genetically engineered natural killer (NK) cells armed with dual aptamers (targeting CD109 and PD-L1) via bio-orthogonal chemistry for enhanced tumor recognition (S3-P-NK); and (3) an Fc-engineered anti-PD-L1 antibody (Atezolizumab/IgG1) that restores antibody-dependent cellular cytotoxicity (ADCC). Crucially, we uncovered a key mechanistic synergy: S3-Lip-DOX treatment, as a stress-adaptive response, upregulates PD-L1 expression on NPC cells. This finding provides a compelling rationale for the integration, turning a potential immune escape mechanism into a therapeutic vulnerability. The complete regimen, comprising S3-Lip-DOX, S3-P-NK, and Atezolizumab/IgG1, demonstrated potent synergistic antitumor effects in vitro and in vivo . This triple-combination therapy not only achieved significant tumor regression but also robustly reprogrammed the innate tumor microenvironment, evidenced by enhanced dendritic cell (DC) maturation and pro-inflammatory macrophage activation. This work establishes a mechanism-driven, modular therapeutic platform that effectively coordinates targeted chemotherapy with innate immunotherapy, holding significant translational potential for solid tumors.
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高影响因子
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