SENSORS AND ACTUATORS B-CHEMICAL
·
2022
·
IF 9.221
Yanyan Li,Chenglong Lin,Yusi Peng,Jun He,Yong Yang
The outbreak of COVID-19 caused by SARS-CoV-2 urges the development of rapidly and accurately diagnostic methods. Here, one high-sensitivity and point-of-care detection method based on magnetic SERS biosensor composed of Fe 3 O 4 -Au nanocomposite and Au nanoneedles array was developed to detect SARS-CoV-2 directly. Among, the magnetic Fe 3 O 4 -Au nanocomposite is applied to capture and separate virus from nasal and throat swabs and enhance the Raman signals of SARS-CoV-2. The magnetic SERS biosensor possessed high sensitivity by optimizing the Fe 3 O 4 -Au nanocomposite. More significantly, the on-site detection of inactivated SARS-CoV-2 virus was achieved based on the magnetic SERS biosensor with ultra-low limit of detection of 100 copies/mL during 15 mins. Furthermore, the contaminated nasal and throat swabs samples were identified by support vector machine, and the diagnostic accuracy of 100% was obtained. The magnetic SERS biosensor combined with support vector machine provides giant potential as the point-of-care detection tool for SARS-CoV-2.
研究领域:
医学诊断
·
纳米技术
·
病毒学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
·
2020
·Vol. 167
·期 12
·126506
·
IF 3.721
Tingting Hao,Chunfeng Zhang,Han Lin,Wenting Wei,Fan Yang,Yangbo Wu,Lingmei Niu,Weijun Kang,Zhiyong Guo
Herein, a dual-mode aptasensor for subnanomolar detection of lead ions Pb2+ based on electrochemiluminescence (ECL) and fast scan voltammetry (FSV) was developed. It could be one-step fabricated using an all-in-one probe Fe3O4@Au-ssDNA&Ru-NH2 which has multiple functions: Fe3O4 core achieves one-step preparation of the aptasensor; Au shell provides a place for assembly and electrochemical reactions; ssDNA can selectively recognize and capture target Pb2+, and ferrocene labeled on it could provide fast scan voltammetric signal and participate in changes of ECL signals; and Ru-NH2 is a signal marker for ECL detection. In presence of target Pb2+, ssDNA folds to form G-quadruplex, and ferrocene labeled on its end approaches to the Au shell surface resulting in easier electron transfer. So the electrochemical signal is enhanced, and ECL quenching occurs. Electrochemical peak current increased about 1000 times at 100 V s−1 using FSV, making its sensitivity compatible with that of ECL to obtain highly sensitive dual-mode detection. Pb2+ could be detected in the concentration range from 10−10 mol l−1 to 10−6 mol l−1 with a limit of quantitation (LOQ) 10−10 mol l−1. Selectivity, stability, reproducibility, accuracy and practicality are good. This extremely simple method provides a valuable idea for developing biosensors for field detection applications.
研究领域:
分析化学
·
生物传感技术
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2018
·
IF 6.434
Qunling Fang,Jianfeng Zhang,Linfeng Bai,Jinyu Duan,Huajian Xu,Ken Cham-Fai Leung,Shouhu Xuan
This work reports a facile one-step method for the fabricating Fe 3 O 4 @Au/polydopamine sandwich-like core-shell nanostructure , in which the Au/polydopamine (Au/PDA) hybrid shell is obtained via an in situ redox-oxidation polymerization between the HAuCl 4 and dopamine. The content of Au nanocrystals, shell thickness, and particle sizes are tunable by varying the experimental parameters. Intriguingly, this general method can be applied for different functional nanostructures such as the β-FeOOH@Au/PDA, SiO 2 @Au/PDA, and CNT@Au/PDA nanocomposites. A possible formation mechanism is proposed and it is found that the surface interaction plays a key role in determining the final nanostructure. The as-prepared Fe 3 O 4 @Au/PDA exhibited eminent catalytic activity on the reduction of 4-nitrophenol. Since the external PDA shell prevents the Au nanocrystals from leaching during the reduction, the cycling activity has been maintained as high as 95% after seven times of catalytic reaction.
研究领域:
材料科学
·
化学
✓ 已验证
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2021
·
IF 9.229
Liying Zhao,Xiaoxiao Song,Xueliang Ouyang,Jiahong Zhou,Jinping Li,Dawei Deng
Bioinspired strategies have recently emerged as novel approaches for designing a functionalized nanovector with enhanced tumor targeting and therapeutic efficacy. Herein, a virus-like Fe3O4/Au@C nanovector is described for programmable drug delivery via hierarchical targeting. Specifically, the virus-like Fe3O4/Au@C nanovector is synthesized via a simple hydrothermal process, and then the spiky surface of which is camouflaged via doxorubicin (DOX)-conjugated polyethylene glycol (PEG), constructing an innovative virus-like core/spherical shell biomimetic nanomedicine (Fe3O4/Au@C-DOX-PEG), which is conducive to improve bioavailability and reduce adverse effects. After systemic administration, the as-prepared nanomedicine is capable of facilitating effective tumor accumulation and deep tumor penetration with the assistance of an external magnetic field and endogenous pH stimuli. Simultaneously, in response to the acidic tumor microenvironment, Fe3O4/Au@C-DOX nanocomposites are released and exhibit excellent performance in cellular internalization through a virus-mimetic rough surface. Furthermore, the in vivo experiments identify that the unique nanomedicine is bestowed with an effective targeting tumor, prominent antitumor efficacy, and reduced systemic toxicity. Such a bioinspired hierarchical targeting nanoplatform holds promising potential for enhanced chemotherapeutic intracellular delivery and tumor theranostics.
研究领域:
纳米技术
·
癌症治疗
·
药物递送系统
·
生物医学工程
✓ 已验证
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 3
·1-12
·
IF 5.3
Sun Danni,Liu Tao,Yao Yiran,Kong Dezhao,Liu Chang,Ye Hua,Zhang Qi,Li Shijie,Li Yaqi,Shi Qiaoqiao
A surface-enhanced Raman scattering (SERS) aptasensor was developed using gold nanostars (Au NSs) and Fe 3 O 4 @Au nanoparticles (NPs) for the highly sensitive detection of aflatoxin B 1 (AFB 1 ). Au NSs were modified by the Raman reporter 4-aminothiophenol (PATP) and then coupled with cDNA to act as the capture probes (Au NSs@PATP-cDNA). Fe 3 O 4 @Au NPs were modified with the AFB 1 aptamer (AFB 1 Apt) and used as signal probes (Fe 3 O 4 @Au NPs-AFB 1 Apt). The SERS peak of PATP at 1078 cm −1 was used for quantitative analysis. When the core-satellite nanostructures (Fe 3 O 4 @Au NPs-AFB 1 Apt/cDNA-Au NSs@PATP) were self-assembled by oligonucleotide hybridization, the SERS intensity was significantly enhanced. When AFB 1 was present, AFB 1 Apt specifically binds to AFB 1 , causing the Fe 3 O 4 @Au NPs-AFB 1 Apt and Au NSs@PATP-cDNA to dissociate, resulting in a decrease in the SERS intensity measured after magnetic separation. Under optimal conditions, the limit of detection (LOD) of AFB 1 can be reduced to 0.24 pg/mL. This is attributed to the high affinity of AFB 1 Apt, excellent magnetic separation characteristics, and multiple SERS hotspots. The assay has been validated to perform well in recovery and accuracy by evaluating spiked samples (rice, corn, and wheat) and positive samples (corn, brown rice, and wheat). Graphical
研究领域:
分析化学
·
食品安全
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
·
2014
·
IF 0.7
Lian-Ming ZHANG,Xiao-Ping WEI,Yan-Xi WEI,Jian-Ping LI,Ying ZENG
Magnetic Fe 3 O 4 nanoparticles were synthesized by co-precipitation method, and then magnetic Fe 3 O 4 @Au nanoparticle were synthesized to improve the affinity of particle surface. L -Cys-GA3 was grafted on the particle surface by self-assembly method, and then dropped it on glass carbon electrode. The MIP/Fe 3 O 4 @Au was further fabricated by electropolymerization of L -Cys. The surface morphology and particle size distribution of the Fe 3 O 4 @Au particle were studied by TEM. The structure and composition of GA3, MIP and nMIP were studied by IR. Under the optimum conditions including 30 cycles of electropolymerization period, 5 min of elution time, 7 min of rebinding time and with acetic acid-methanol (1:8, V / V ) mixed solution as eluent, high stability and good recognition ability for GA3 were obtained through the sensor. The results showed that the concentration of GA3 in the range of 1.0 × 10 −11 −1.0 × 10 −8 M had a linear relationship with the intensity of the oxidation peak current, with the detection limit of 2.6 × 10 −12 M. The sensor was successfully used for the determination of GA3 in beer sample.
研究领域:
分析化学
·
植物激素检测
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2018
·
IF 5.123
Chunhong Zhu,Wanying Zhu,Lei Xu,Xuemin Zhou
Herein, a novel magnetic biocomposite (Fe 3 O 4 @Au-S1/S2) was applied to analyze thrombin. The Fe 3 O 4 @Au-S1/S2 consisted of Fe 3 O 4 @Au nanoparticles (Fe 3 O 4 @Au NPs) as carriers for magnetic separation and magnetic field-induced self-assembly, thiolated complementary strand (S1) anchored based on Au-S bond and thrombin binding aptamer (S2) as a recognition element. As a redox indicator , methylene blue (MB) can be adsorbed to DNA anchored on the surface of Fe 3 O 4 @Au NPs by electro-static interaction. In the absence of thrombin, MB were adsorbed on double-stranded DNA (S1/S2) which anchored on Fe 3 O 4 @Au NPs and a high electrochemical signal of MB was recorded by Differential pulse voltammetry . Conversely, the complementary strand (S1) exposed after thrombin competitively bonded with aptamer. The introduction of Pb 2+ -dependent DNAzyme (S3) split S1 at specific rA site, resulting in the significantly decreased adsorption capacity of MB. Thus, the thrombin detection could be recorded by monitoring the electrochemical signal reduction of MB through incubation of thrombin with S3. This method exhibited a high sensitivity toward thrombin with a broad linear range from 5 pmol L −1 to 5 nmol L −1 and a limit of detection of 1.8 pmol L −1 .
研究领域:
分析化学
·
生物传感器
·
电化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2017
·
IF 7.504
Peng Miao,Yuguo Tang,Lei Wang
Driven by the urgent need to detect trace heavy metal ions in various real water samples, this article demonstrates for the first time an electrochemical biosensor based on DNA modified Fe3O4@Au magnetic nanoparticles (NPs). Three DNA probes are designed to contain certain mismatched base pairs. One is thiolated and modified on the surface of Fe3O4@Au NPs (DNA 1). The other two probes (DNA 2 and 3) are labeled with two independent electrochemical species. Stable structures of cytosine–Ag+–cytosine and thymine–Hg2+–thymine formed in the presence of Ag+ and Hg2+ can assist the hybridization of DNA 1/DNA 2 and DNA 1/DNA 3, which locate corresponding electrochemical species onto the surface of the magnetic NPs. The achieved nanocomposites are then used as selective electrochemical probes for the detection of heavy metal ions by recording the square wave voltammetry signals. Simultaneous detection of Ag+ and Hg2+ is demonstrated without significant interference, and their individual high sensitivities are fundamentally preserved, which meet the requirements of U.S. Environmental Protection Agency (USEPA). Furthermore, the proposed method has been challenged by various real water samples. The results confirm the DNA modified magnetic NPs based sensing method may have potential applications for the monitoring of heavy metal ions in real sample analysis.
研究领域:
生物传感技术
·
环境科学
·
纳米材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ELECTROANALYSIS
·
2017
·Vol. 29
·期 9
·2098-2105
·
IF 2.851
Fang Shang,Yuan Liu,Sui Wang,Yufang Hu,Zhiyong Guo
An ultrasensitive electrochemiluminescence (ECL) immunosensor for the detection of tetrodotoxin (TTX) is proposed, which are composed of the branched poly-(ethylenimine) (BPEI) functionalized graphene (BGNs)/Fe 3 O 4 -Au magnetic capture probes and luminol-capped gold nanocomposites (luminol-AuNPs) as the signal tag. Herein, a typical sandwich immunecomplex was constructed on the glassy carbon electrode. The BGNs/Fe 3 O 4 -Au hybrids could efficiently conjugate primary antibody via the Au−S chemical bonds or Au−N chemical bonds and rapidly separate under external magnetic field. The introduction of BPEI to GO could enhance the luminol-ECL intensity. Meanwhile, the multifunctional nanocomposites have been proved with good water-solubility, excellent electron transfer, outstanding stability, etc. The luminescent luminol-AuNPs, a high efficient electrochemiluminescence marker, can be assembled on the second antibody, which can produce the ECL signal to achieve the determination of TTX. This proposed ECL immunosensor with a linear range from 0.01–100 ng/mL can be applied in the detection of TTX in real samples with satisfactory results.
研究领域:
分析化学
·
纳米技术
·
毒理学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2021
·Vol. 45
·期 41
·19593-19604
·
IF 3.591
Yaoyu Tan,Huan Jiang,Baihui Wang,Xia Zhang
Surface-enhanced Raman scattering (SERS) is an ultrasensitive fingerprint spectroscopy technique. The combined application of SERS and nanozymes has shown excellent prospects in the field of biomedical diagnosis. In the present study, polyaniline@MoS2@Fe3O4@Au (PANI@MoS2@Fe3O4@Au) nanozymes were successfully prepared using hydrothermal reactions. PANI@MoS2@Fe3O4@Au nanozymes have not only dramatically enhanced the peroxidase-like activity but also significantly enhanced the SERS performance. We also studied the effect of different concentrations of HAuCl4·4H2O on the SERS activity of nanozymes and found that PANI@MoS2@Fe3O4@Au nanozymes prepared using 2 mg mL−1 HAuCl4·4H2O solution exhibited the best SERS performance. We used SERS to study the enzyme kinetics of the nanozymes, and the results showed that the nanozymes have a strong affinity for H2O2 and TMB. Finally, we combined PANI@MoS2@Fe3O4@Au nanozymes and glucose oxidase (GOx) to establish a SERS glucose biosensor, which has the advantages of ultrasensitivity, high-precision, and a wide linear response range. The linear response range of glucose is 10−11–10−3 M, and the detection limit is 10−12 M. More importantly, we have successfully determined the glucose content in human serum samples with satisfactory accuracy. This work provides an essential new strategy for designing composite nanozymes for biocatalysis and provides a new idea for constructing high-sensitivity SERS biosensors.
研究领域:
生物医学诊断
·
纳米材料科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
MOLECULES
·
2021
·Vol. 26
·期 11
·3180
·
IF 4.412
Yina Cai,Binxue Ren,Chifang Peng,Cunzheng Zhang,Xinlin Wei
New nanocomposites, Fe3O4@Au–FITC, were prepared and explored to develop a fluorescent detection of Pb2+. The Fe3O4@AuNPs–FITC nanocomposites could be etched by Pb2+in the presence of Na2S2O3, leading to fluorescence recovery of FITC quenched by Fe3O4@Au nanocomposites. With the increase of Pb2+concentration, the fluorescence recovery of Fe3O4@AuNPs–FITC increased gradually. Under optimized conditions, a detection limit of 5.2 nmol/L of Pb2+with a linear range of 0.02–2.0 µmol/L were obtained. The assay demonstrated negligible response to common metal ions. Recoveries of 98.2–106.4% were obtained when this fluorescent method was applied in detecting Pb2+spiked in a lake-water sample. The above results demonstrated the high potential of ion-induced nanomaterial etching in developing robust fluorescent assays.Keywords:gold nanoparticles;magnetic nanoparticles;FRET;nanomaterial etching;lead ions
研究领域:
分析化学
·
环境科学
✓ 已验证
DOI
PubMed
高影响因子
MOLECULES
·
2019
·Vol. 24
·期 17
·3039
·
IF 3.06
Yijie Mao,Yu Zhang,Wei Hu,Weiwei Ye
Histamine can be formed by enzymatic decarbonylation of histidine, which is an important indicator of seafood quality. A rapid and sensitive assay method is necessary for histamine monitoring. A fluorescence resonance energy transfer (FRET) assay system based on a carbon dot (CD)-modified nanoporous alumina membrane and Fe3O4@Au magnet nanocomposites has been developed for histamine detection in mackerel fish. CDs immobilized on nanoporous alumina membranes were used as donors, which provided a fluorescence sensing substrate for histamine detection. Fe3O4@Au magnet nanocomposites can not only act as acceptors, but also concentrate histamine from fish samples to increase detection sensitivity. Histamine was detected by the fluorescence signal changes of CDs capturing histamine by an immune reaction. The fluorescence signals of CDs were quenched by Fe3O4@Au magnet nanocomposites via the FRET mechanism. With an increase of histamine, the fluorescence intensity decreased. By recording fluorescence spectra and calculating intensity change, histamine concentration can be determined with a limit of detection (LOD) of 70 pM. This assay system can be successfully applied for histamine determination in mackerel fish to monitor the fish spoilage process in different storage conditions. It shows the potential applications of CDs-modified nanoporous alumina membranes and Fe3O4@Au magnet nanocomposites-based biosensors in the food safety area.Keywords:fluorescence resonance energy transfer;CDs;nanoporous alumina membrane;food safety
研究领域:
生物传感
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）