Nanomaterials
·
2024
·Vol. 14
·期 19
·1579
·
IF 4.4
Liying Jiang,Dongyang Li,Mingxing Su,Yirong Qiu,Fenghua Chen,Xiaomei Qin,Lan Wang,Yanghai Gui,Jianbo Zhao,Huishi Guo,Xiaoyun Qin,Zhen Zhang
Cardiac troponin I (cTnI) monitoring is of great value in the clinical diagnosis of acute myocardial infarction (AMI). In this paper, a highly sensitive electrochemical aptamer sensor using polystyrene (PS) microspheres as the electrode substrate material in combination with Prussian blue (PB) and gold nanoparticles (AuNPs) was demonstrated for the sensitive and label-free determination of cTnI. PS microspheres were synthesized by emulsion polymerization and then dropped onto the glassy carbon electrode (GCE); PB and AuNPs were electrodeposited on the electrode in corresponding electrolyte solutions step by step. The PS microsphere substrate provided a large surface area for the loading mass of the biological affinity aptamers, while the PB layer improved the electrical conductivity of the modified electrode, and the electroactive AuNPs exhibited excellent catalytic performance for the subsequent electrochemical measurements. In view of the above mentioned AuNPs/PB/PS/GCE sensing platform, the fabricated label-free electrochemical aptamer sensor exhibited a wide detection range of 10 fg/mL~1.0 μg/mL and a low detection limit of 2.03 fg/mL under the optimal conditions. Furthermore, this biosensor provided an effective detection platform for the analysis of cTnI in serum samples. The introduction of this sensitive electrochemical aptamer sensor provides a reference for clinically sensitive detection of cTnI.
研究领域:
生物医学工程
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分析化学
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临床诊断
·
纳米技术
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产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2016
·Vol. 8
·期 10
·2218-2225
·
IF 1.915
Yanjun Zhang,Xiuhui Liu,Lin Li,Zhipan Guo,Zhonghua Xue,Xiaoquan Lu
An electrochemical sensor for the detection of paracetamol (PAT) was reported employing multiwalled carbon nanotubes (MWCNTs) and the fourth generation poly(amidoamine) dendrimers (G4.0 PAMAM). The strategy of this work lies on the formation of a stable covalent linkage between carboxylated MWCNTs and amine terminated G4.0 PAMAM via covalent layer-by-layer (LbL) self-assembly, which provides larger surface areas for efficient adsorption of PAT. The constructed electrode was characterized first by Fourier transform infrared spectroscopy and scanning electron microscopy, and then the electrochemical behavior of PAT on the (MWCNTs–G4.0)6/GCE was investigated in detail by cyclic voltammetry, electrochemical impedance spectroscopy and chronocoulometry. Under optimal conditions, the experimental results demonstrated that the constructed sensor exhibited excellent catalytic activity toward PAT, and a wide linear range from 3.0 × 10−7 to 2.0 × 10−4 M was obtained with a limit of detection (LOD) of 1.0 × 10−7 M (S/N = 3). Moreover, it could also be applied to the determination of PAT in commercial tablets and human serum, and the satisfactory results confirm the applicability of this sensor in practical analysis.
研究领域:
分析化学
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纳米材料科学
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药物分析
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产品关联
DOI
高影响因子
CELLULOSE
·
2025
·
IF 4.8
Al Kiey Sherief A.,Baseer Rasha A.,Kamel Samir
The simultaneous detection of ascorbic acid (AA), dopamine (DA), and uric acid (UA) is crucial for biomedical and clinical diagnostics. Herein, we present a highly sensitive and selective electrochemical sensor based on a modified glassy carbon electrode (GCE) functionalized with hydroxypropyl methylcellulose (HPMC), polyvinylpyrrolidone (PVP), and 3D reduced graphene oxide/pentaerythritol (3D rGO/PE). The structural and morphological properties of the composite were characterized using Fourier-transform infrared spectroscopy (FTIR), Raman spectroscopy, X-ray diffraction (XRD), and scanning electron microscopy (SEM). Electrochemical analysis demonstrated the remarkable electrocatalytic activity of 3D rGO/PE/PVP@HPMC/GCE for the oxidation of AA, DA, and UA, with peak potential separations of 214, 133, and 346 mV for DA-AA, DA-UA, and UA-AA, respectively. Under optimized conditions, the sensor exhibited wide linear detection ranges of 4.0 µM to 1.0 mM (AA), 0.2–100 µM (DA), and 1.0–100 µM (UA), with detection limits of 0.82, 0.002, and 0.025 M, respectively. The sensor demonstrated excellent sensitivity, selectivity, reproducibility, and stability, making it a promising platform for real-sample analysis.
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DOI
高影响因子
International Journal of Electrochemical Science
·
2023
·
IF 1.5
Ming Zhou,Yong Xu,Rong Zhong,Yurong Wang
A glassy carbon electrode modified by carboxyl-functionalized reduced graphene oxide (ERGO-COOH/GCE) was developed by electrochemical deposition method. The electrochemical behavior of caffeic acid (CA) on the ERGO-COOH/GCE was studied, and an electroanalytical method for the detection of CA in tablets was established. The effects of the modification parameters, the pH of the buffer solution and the scanning rate on the electrochemical behavior of CA were investigated by cyclic voltammetry (CV). The results revealed that modifying the GCE by electrochemical deposition facilitated a more sensitive response to CA on the modified electrode than modifying it by drop coating. The peak current of CA on the ERGO-COOH/GCE was significantly higher than that on the bare glassy carbon electrode (GCE) over five scan cycles, and the optimized the concentration of GO-COOH, the scanning cycles, and the pH were 4 mg·mL -1 , 5 cycles, and 2.0, respectively. The redox reaction of CA on the modified electrode was a quasi-reversible process controlled by adsorption and involved the transfer of two electrons and two protons. The ERGO-COOH/GCE showed a linear response to CA in the range of 0.05 ~ 20 μM with a limit of detection of 8 nM ( S/N =3). The modified electrode exhibited good repeatability and reproducibility and anti-interference performance. The ERGO-COOH/GCE was applied to the detection of CA in caffeic acid tablets with a recovery of 97.7% ~ 108%.
研究领域:
分析化学
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电化学
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生物传感
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药物分析
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纳米材料
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电分析化学
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药剂学
✓ 已验证
DOI
高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 6
·1-10
·
IF 5.3
Zhong Yujun,Li Zhiyang,Zhang Anlin,Peng Yuqing,Zhou Hao,Wang Bin,Xie Lianwu,Guo Yaping
A novel electrochemical sensor, MIP/Cu-MOF/rGO/AuNPs/GCE, was developed by depositing gold nanoparticles, coating Cu-MOF/GO on the surface of glassy carbon electrode (GCE) before electroreducing graphene oxide (GO) to rGO and covering molecularly imprinted membrane by electropolymerization for highly sensitive detection of electroneutral organophosphorus pesticide residues in agricultural product. Cyclic voltammetry, differential pulse voltametry, scanning electron microscopy, energy-dispersive spectroscopy, and atomic force microscopy were used to characterize the imprinted sensor. Several key factors such as chitosan concentration, suspension volume, pH of polymerization solution, and polymerization scanning rate during preparation of the imprinted sensor were optimized in detail. When electroneutral phosmet was used as a template, the linear range of MIP/Cu-MOF/rGO/AuNPs/GCE for detecting phosmet was 1.00 × 10 −14 –5.00 × 10 −7 mol/L with the limit of detection of 7.20 × 10 −15 mol/L at working potentials of − 0.2 to 0.6 V. The selectivity, reproducibility, and repeatability of MIP/Cu-MOF/rGO/AuNPs/GCE were all acceptable. The recoveries of this method for determining phosmet in real samples ranged from 94.2 to 106.5%. The MIP/Cu-MOF/rGO/AuNPs/GCE sensor could be applied to detect electroneutral pesticide residues in organisms and agricultural products. Graphical Abstract
研究领域:
分析化学
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环境科学
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农业科学
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2017
·
IF 5.401
Wanqing Zhang,Lukuan Zong,Guangwei Geng,Yuanchao Li,Yuping Zhang
A novel MIP/MIL-101 (Cr)/MoS 2 /GCE sensor was fabricated for the determination of quercetin (Qu), based on MIL-101 (Cr)/MoS 2 modified GCE , followed by molecularly imprinted polymer (MIP) immobilized on modified electrode. Nanoscale materials of MIL-101 (Cr) and MoS 2 as electrochemical sensor modifiers were synthesized via hydrothermal methods The physical characterizations of the prepared MIL-101 (Cr), MoS 2 and MIL-101 (Cr)/MoS 2 were examined by FTIR, XRD, BET and TEM. The influencing parameters controlling the performance of the prepared sensor were in detailed studied. Under the optimized conditions, the electrochemical behavior of Qu at the MIP/MIL-101 (Cr)/MoS 2 /GCE sensor was investigated by using differential pulse voltammetry (DPV) method. The proposed sensor possessed desirable linear relationship between the peak currents and the Qu concentrations. Two linear concentration ranges can be observed at the MIP/MIL-101 (Cr)/MoS 2 /GCE sensor in the ranges of 0.1 to 10.5 μM and 10.5 to 700 μM, with a detection limit of 0.06 μM in phosphate buffer solution (PBS, pH = 3.5) (S/N = 3). Additionally, the MIP sensor presented excellent reproducibility, stability and selectivity. Furthermore, the imprinted sensor was also successfully applied for the determination of Qu in honey samples and received satisfactory recoveries.
研究领域:
分析化学
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材料科学
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食品科学
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产品关联
DOI
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2022
·
IF 5.518
Zhou Liuzhu,Sankar Sekar,Jin Chen,Sejoon Lee,Deuk Young Kim,Ramalingam Manikandan
Here, we report a simple one-step preparation of polyrutin/silver nanoparticles (AgNPs) on the glassy carbon electrode (GCE) surface using an electrochemical deposition/polymerization technique. As prepared polyrutin/AgNPs/GCE has been characterized by different techniques such as SEM with elemental morphing, TEM with EDAX , XRD , ATR-IR to confirm the materials formation. Further, electrochemical behavior of the polyrutin/AgNPs/GCE was tested by cyclic voltammetry , EIS and differential pulse voltammetric techniques. As fabricated polyrutin/AgNPs/GCE was used for simultaneous detection of Pb(II) and Cd(II)ions using anodic stripping voltammetric techniques and stripping analysis of Pb(II) and Cd(II)ions analytical parameters like pH, preconcentration time were studied. The polyrutin/AgNPs/GCE has showed a linear concentration range from 29 nM to 140 nM and 57–203 nM with detection limit of 3 nM and 10 nM for Pb(II) Cd(II)ions respectively. Further, the modified electrode was tested towards detection of these heavy metal ions in different environmental samples and the found excellent recovery results were further correlated with ICP-MS results.
研究领域:
环境科学
·
分析化学
✓ 已验证
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Jiawen Hong,Junhua Zhao,Hexin Wang,Jiesai Ma,Ruo Wang,Shen Wang,Yangjun Mao,Wenfeng Shen,Lianbang Wang
The presence of the toxic synthetic precursor and degradation product, 4-aminophenol (4-AP), in acetaminophen (ACOP) formulations poses significant health risks, necessitating robust analytical methods for quality control. Herein, a novel electrochemical sensor was developed based on a composite material synthesized by growing MOF-808 in situ on silver nanowires (AgNWs) via a one-pot method. The MOF-808/AgNWs composite synergistically combines the properties of both components, resulting in a sensor with excellent performance: wide linear ranges (0.1–400 μM), low detection limits (0.0175 μM for 4-AP and 0.0561 μM for ACOP), and high selectivity and stability. At the same time, the sensor also showed outstanding repeatability, reproducibility, stability and selectivity. The method was successfully applied to determine both analytes in pharmaceutical tablets and environmental water samples, achieving satisfactory recoveries. This work provides a simple, rapid, and highly sensitive platform for monitoring ACOP and 4-AP, holding great promise for pharmaceutical and environmental applications.
研究领域:
分析化学
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电化学
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药学
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环境科学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2013
·
IF 3.535
Yancai Li,Shuqing Feng,Shunxing Li,Yayun Zhang,Yanmei Zhong
This work presents an excellent strategy to assemble a new carboxylic acid functionalized multi-wall carbon nanotubes (CMWCNTs) thin film via layer by layer (LBL) covalent bond by using ethylenediamine as an arm linker. Scanning electron microscopy (SEM) and Fourier transform infrared (FT-IR) spectroscopy were used to characterize the assembly process. Electrochemical studies indicated that the modified electrode possesses a remarkably electrocatalytic activity toward paracetamol at a lower potential compared with the bare glass carbon electrode (GCE), and without the need for redox mediators. The thickness of the film assembled CMWCNTs is easily controlled by changing the numbers of the cycles in the LBL assembly process, which also effectively tuned the electrocatalytic activity of the film toward paracetamol. The experiments result display that the six layers film performs the best electrocatalytic activity to paracetomol. The constructed electrode with six layers showed a good sensitivity of 2.293 μA M −1 cm −2 in the linear range of 1–200 μM, and a detection limit of 0.092 μM ( S / N = 3). Furthermore, the CMWCNTs film also showed good selectivity and excellent stability for the determination of paracetamol.
研究领域:
分析化学
·
电化学
·
纳米生物技术
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药物分析
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生物传感器
✓ 已验证
DOI
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2026
·
IF 5.9
Furong Zhu,Keyi Xu,Hongjie Ling,Jiaxi Chao,Jiayin Li,Jun Liu,Chun Yang
3D/2D Bi 2 O 2 CO 3 /rGO composites were synthesized successfully. • Bi 2 O 2 CO 3 /rGO/GCE were used for high-sensitive electrochemical CAP detection. • Wide linear range (0.001–100 μM) and low LOD of 0.362 nM (S/N = 3) are obtained. Chloramphenicol (CAP) remains prevalent in aquaculture despite its classification as a Group 2 A carcinogen. Given the associated health risks, establishing robust detection methods for CAP is imperative. Herein, we innovatively propose the three-dimensional (3D) flower-like Bi 2 O 2 CO 3 /rGO composites for CAP electrochemical sensing. The best sensing parameters (pH, scan rate, accumulation conditions) were systematically investigated. As a result, the Bi 2 O 2 CO 3 /rGO/GCE sensor exhibits a peak current density of 381.91 µA/cm 2 , ultra-low detection limit (0.362 nM), and two wide linear ranges (0.001–10 μM, 10–100 μM), enabling both trace and high-concentration quantitative detection of CAP. These high sensing performances could be ascribed to the synergistic effect between rGO and Bi 2 O 2 CO 3 , which improves the stability of Bi 2 O 2 CO 3 /rGO composites. Their interfacial interaction accelerates electron transfer, lowers activation energy, and forms an efficient 3D electron/mass transport network. This sensor also applied in detecting CAP in lake water and milk samples with satisfied recovery rates. These findings underscore the Bi 2 O 2 CO 3 /rGO/GCE as a promising platform for sensitive CAP monitoring in environmental and food safety applications.
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DOI
PubMed
高影响因子
MATERIALS RESEARCH INNOVATIONS
·
2022
·
IF 0
Shridevi Salagare,Prashanth Shivappa Adarakatti,Suma B P,Yarradoddappa Venkataramanappa
A nitrite sensing platform has been constructed using a TiO2-reduced graphene oxide (RGO) nanocomposite incorporated into a glassy carbon electrode. Reduced graphene oxide is integrated in a simple technique for the manufacture of titanium dioxide (TiO2). Spectroscopic and electrochemical techniques have all been used to characterise the TiO2- RGO nanocomposite. In 3 mM and pH 6.0 phosphate buffer solutions, the TiO2-reduced graphene oxide nanocomposites tailored glassy carbon electrode demonstrated outstanding electro-catalytic nitrite oxidation activity. The square root of scan rate is also proportional to the oxidation peak current, showing that the nitrite redox reaction involves a diffusion-controlled mechanism. Using linear sweep voltammetry, the sensitivity and detection limit for nitrite at the modified electrode were determined to be 4.853 μA/μM cm2 and 0.006 µM respectively, over a concentration range of 10 - 2000 µM. Further, the nanocomposite is used to measure NO2- in water and buttermilk samples quantitatively.
研究领域:
分析化学
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材料科学
·
环境科学
✓ 已验证
产品关联
DOI
ANALYTICAL SCIENCES
·
2022
·Vol. 38
·期 9
·1181-1188
·
IF 1.967
Wang Cheng-Yan,Ge Qingmei,Jiang Nan,Cong Hang,Tao Zhu,Liu Mao,Fan Ying
Metal–organic frameworks (MOFs) including cucurbit[7]uril block (Q[7]·HAuCl 4 ) were employed to develop a diphenylamine (DPA) sensor in electrochemical method, the presence of HAuCl 4 improved the conductivity of the macrocyclic compound. To further enhance of the sensitivity, Au nanoparticles were inserted between the surface of glassy carbon electrode and Q[7]·HAuCl 4 MOFs (GCE-AuNPs-Q[7]·HAuCl 4 ). Cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and differential pulse voltammetry (DPV) were applied for evaluation on the electrochemical behavior. For the electrochemical inertness of DPA, a label-free electrochemical sensor in 5 mM K 3 [Fe(CN) 6 ] solution was achieved, to produce a limit of detection as low as 4.6 µM in a linear range of 5–1000 µM with good reproducibility, high stability and acceptable anti-interference ability. Application of the proposed electrode for the quantitative determination of DPA in tap water and apple juice confirms its real value. Graphical abstract
研究领域:
分析化学
·
材料科学
·
电化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）