MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Zhongshuai Zhao,Ran Li,Ying Zhu,Zhongde Liu
Hydrogen sulfide (H 2 S), playing unique role on signal transmission in various physiological processes, the rapid and accurate monitoring of it is particularly concerned. The drawbacks of fluorescence probe for H 2 S, namely poor selectivity or long response time, have always existed. As a test case, here headspace separation combined fluorescence strategy was attempted, on basis of H 2 S stimuli-responsive AMP/Tb 3+ /Ag + or GMP/Tb 3+ /Ag + coordination polymer probes. The fluorescence intensity of them is inversely proportional to the concentration of it, due to Ag-S in situ coordination and partial formation of Ag 2 S. Using the hybrid analysis, a quick response of less than 2.0 min and a high selectivity could be obtained. The signal change (Δ I ) of AMP/Tb 3+ /Ag + or GMP/Tb 3+ /Ag + showed good linearity within the range of 0.5–100 μM and 1.0–150 μM, along with a detection limit (3 σ ) of 0.2 and 0.4 μM, respectively. When utilized to determine the sulfide level in real samples, it shows recoveries from 96.4 % to 102.3 % with relative standard derivation ( RSD ) less than 3.0 %. The combination strategy of headspace separation and fluorescence quantitative analysis was verified to effectively eliminate potential interference such as other coexisting non-volatile thiol-containing species, providing a novel avenue to flexibly design the volatile component analysis with high selectivity in complex sample matrixes.
研究领域:
分析化学
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环境科学
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生物化学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2024
·
IF 12.2
Haibo Wang,Chisheng Hu,Yukang Li,Yun Shen,Jianhua Guo,Baoyou Shi,Pedro J.J. Alvarez,Pingfeng Yu
Despite the growing prevalence of nanoplastics in drinking water distribution systems, the collective influence of nanoplastics and background nanoparticles on biofilm formation and microbial risks remains largely unexplored. Here, we demonstrate that nano-sized polystyrene modified with carboxyl groups (nPS) and background magnetite (nFe 3 O 4 ) nanoparticles at environmentally relevant concentrations can collectively stimulate biofilm formation and prompt antibiotic resistance . Combined exposure of nPS and nFe 3 O 4 by P. aeruginosa biofilm cells stimulated intracellular reactive oxidative species (ROS) production more significantly compared with individual exposure. The resultant upregulation of quorum sensing (QS) and c-di-GMP signaling pathways enhanced the biosynthesis of polysaccharides by 50 %− 66 % and increased biofilm biomass by 36 %− 40 % relative to unexposed control. Consistently, biofilm mechanical stability (measured as Young’s modulus) increased by 7.2–9.1 folds, and chemical stress resistance (measured with chlorine disinfection) increased by 1.4–2.0 folds. For P. aeruginosa , the minimal inhibitory concentration of different antibiotics also increased by 1.1–2.5 folds after combined exposure. Moreover, at a microbial community-wide level, metagenomic analysis revealed that the combined exposure enhanced the multi-species biofilm’s resistance to chlorine, enriched the opportunistic pathogenic bacteria, and promoted their virulence and antibiotic resistance. Overall, the enhanced formation of biofilms (that may harbor opportunistic pathogens) by nanoplastics and background nanoparticles is an overlooked phenomenon, which may jeopardize the microbial safety of drinking water distribution systems.
研究领域:
环境科学
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微生物学
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纳米技术
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水质管理
✓ 已验证
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2021
·
IF 9.229
Yunsu Ma,Huanghuang Xu,Bo Sun,Silin Du,Shuai Cui,Li Zhang,Ning Ding,Dongzhi Yang
The combination of photodynamic therapy (PDT) and chemodynamic therapy (CDT) has been continuously explored in the antibacterial aspect and has achieved more effective antibacterial effect than a single therapy. We design a pH-responsive O2 and H2O2 self-supplying zeolitic imidazolate framework-67 (ZIF-67) nanosystem for PDT/CDT of wound infection. Under the acidic inflammatory conditions, ZIF-67 can degrade to produce Co2+ and release CaO2 and graphene quantum dots (GQDs). The exposed CaO2 reacted with water to generate H2O2 and O2. The self-supplied O2 alleviates hypoxia at the site of inflammation and enhances external light-initiated GQD-mediated PDT, while H2O2 was catalyzed by endogenous Co2+ to produce hydroxyl radicals for Co2+-triggered CDT. In vitro and in vivo experiments confirm that CaO2/GQDs@ZIF-67 has a combined PDT/CDT effect. The antibacterial mechanism indicates that bacteria post-treated with CaO2/GQDs@ZIF-67 may be sterilized by reactive oxygen species-mediated oxidative stress and the leakage of bacterial contents. The experiments also find that CaO2/GQDs@ZIF-67 may activate the immune response and enhance the therapeutic effect by activating the cyclic GMP–AMP synthase–stimulator of interferon genes signaling pathway.
研究领域:
纳米技术
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生物医学工程
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药学
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传染病
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材料科学
✓ 已验证
DOI
PubMed
高影响因子
Cell Death & Disease
·
2019
·Vol. 10
·期 10
·1-14
·
IF 5.959
Li Zhongwen,Wu Jun,Wang Lei,Han Weifang,Yu Juan,Liu Xin,Wang Yukai,Zhang Ying,Feng Guihai,Li Wei,Stacey Glyn Nigel,Gu Qi,Hu Baoyang,Wang Liu,Zhou Qi,Hao Jie
Hepatocytes have been successfully generated from human pluripotent stem cells (hPSCs). However, the cost-effective and clinical-grade generation of hepatocytes from hPSCs still need to be improved. In this study, we reported the production of functional hepatocytes from clinical-grade human embryonic stem cells (hESCs) under good manufacturing practice (GMP) requirements. We sequentially generated primitive streak (PS), definitive endoderm (DE), hepatoblasts and hepatocyte-like cells (HLCs) from hESCs in the different stages with completely defined reagents. During hepatoblast differentiation, dimethyl sulfoxide (DMSO), transferrin, L-ascorbic acid 2-phosphate sesquimagnesium salt hydrate (Vc-Mg), insulin, and sodium selenite were used instead of cytokines and FBS/KOSR. Then, hepatoblasts were differentiated into HLCs that had a typical hepatocyte morphology and possessed characteristics of mature hepatocytes, such as metabolic-related gene expression, albumin secretion, fat accumulation, glycogen storage, and inducible cytochrome P450 activity in vitro. HLCs integrated into the livers of Tet-uPA Rag2 –/– Il2rg –/– (URG) mice, which partially recovered after transplantation. Furthermore, a series of biosafety-related experiments were performed to ensure future clinical applications. In conclusion, we developed a chemically defined system to generate qualified clinical-grade HLCs from hESCs under GMP conditions. HLCs have been proven to be safe and effective for treating liver failure. This efficient platform could facilitate the treatment of liver diseases using hESC-derived HLCs transplantation.
研究领域:
干细胞生物学
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再生医学
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肝病治疗
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移植学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Materials Today Nano
·
2024
·
IF 8.2
Chen Wang,Qiliner Feng,Hongli Lu,Qiubei He,Peng Zhang,Junjie Tang,Zhuoyin Liu,Jie Liu,Baizhu Chen
The mitochondrial energy metabolic reprogramming of tumors leads to the hypoxic, acidic, and immunosuppressive microenvironment. To regulate the reprogrammed mitochondrial energy metabolism and the abnormal tumor microenvironment (TME) intracellularly and extracellularly, herein, we purposely constructed the metabolic regulating and immunological activating nanocomposites (NCs) formed by the manganese dioxide (MnO 2 ) nanoparticles loaded with lactate oxidase (LOX), dichloroacetic acid (DCA) and SR-717, camouflaged by the cell membrane of tumors. After the NCs targeting to the tumors, the loaded DCA inhibited the glycolysis and triggered the produced pyruvate into mitochondria for further degradation. DCA also reduced the amount of CD39 and CD73, thereby promoting the accumulation of ATP intracellularly. The cascade of MnO 2 and LOX catalyzed the lactate to pyruvate together with oxygen for the relief of the hypoxic and immunosuppressive TME. The regulation of the mitochondrial metabolism together with the degradation of lactate contributed to activating the immune cells. Meanwhile, the loaded SR-717 together with the release of Mn 2+ activated the cyclic guanosine monophosphate adenosine monophosphate synthase/interferon gene stimulator signaling pathway for efficient antitumor effects and immunotherapy. Taken together, the designed multifunctional nanocomposites realized an effective tumor immunotherapy by regulating the reprogrammed mitochondrial energy metabolism intracellularly, relieving the abnormal TME and activating the innate antitumor immunological effects extracellularly. Our work established an approach to the enhanced immunotherapy by regulating the reprogrammed metabolism for efficient cancer treatment.
研究领域:
癌症生物学
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免疫学
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纳米技术
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生物化学
·
药理学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）