Journal of Materials Chemistry B
·
2013
·Vol. 1
·期 26
·3304-3313
·
IF 0
Tianyi Wu,Qingchun Zhang,Weiping Ren,Xiang Yi,Zubin Zhou,Xiaochun Peng,Xiaowei Yu,Meidong Lang
Infection of the bone (osteomyelitis) remains one of the most challenging problems in the field of orthopedic surgery. The limitations of systemic antibiotics administration include undesired side effects, systemic toxicity, patient discomfort, and development of bacterial resistance. In this study, we developed a bactericidal gentamicin-doped beta-tricalcium phosphate (TCP) scaffold reinforced with a gelatin/genipin hydrogel (G-TCP). Our data showed that the gentamicin-doped G-TCP had a much longer drug releasing period, while the gentamicin was completely released from pure TCP cements (B-TCP) within one day. In addition, the release profile of G-TCP exhibited an initial burst followed by a zero-order release. One standard strain, Staphylococcus aureus (S. aureus, ATCC25923) was selected to evaluate the antibacterial activity and therapeutic effect of this scaffold. G-TCP significantly inhibited growth of S. aureus both in vitro and in vivo. In a rat osteomyelitis model, osteomyelitis could be totally cured after implantation of G-TCP for three weeks. We propose that the gelatin/genipin–gentamicin TCP scaffold represents one of the promising gentamicin releasing bone scaffolds in treating osteomyelitis.
研究领域:
骨科手术
·
药剂学
·
生物材料
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DOI
PubMed
FOOD HYDROCOLLOIDS
·
2021
·
IF 9.147
Cen Zhang,Peng Wang,Jiawen Li,Hui Zhang,Jochen Weiss
Core-shell nanofibers were electrospun from bilayer gelatin/gum arabic corn oil-in-water emulsions crosslinked by genipin. The crosslinking increased the apparent viscosity and induced a predominantly elastic behavior of the emulsions, which favored the formation of a more stable emulsion system and then electrospun fibers with larger diameters. These fibers had improved surface hydrophobic and mechanical properties, and did not thermally decompose until heated to 250 ° C, which might be associated with the chemical bonds formed between primary amines on the protein chains. The encapsulation efficiency of corn oil in the fibers at different crosslinking time was higher than 86% without any significant change during 7 days of storage at room temperature. Thus, this work provides a genipin crosslinking strategy to the development of emulsion-based fibrous mats with desired mechanical and surface hydrophobic properties for food applications of bioactive encapsulation and controlled release.
研究领域:
食品科学
·
生物材料学
✓ 已验证
产品关联
DOI
高影响因子
PROGRESS IN ORGANIC COATINGS
·
2022
·
IF 6.206
Zhe Xiao,Lin-Hao Liu,Teng Liu,Dan Yang,Xiang Jia,Yu-Kun Du,Shuo-Qi Li,Wen-Jiu Yang,Yong-Ming Xi,Rong-Chang Zeng
Polyelectrolyte-coated magnesium alloy was prepared by layer-by-layer assembly with poly (allylamine hydrochloride) (PAH) and sodium alginate (ALG). The potential influence of genipin as a crosslinking agent on the microscopic features and corrosion resistance of the coatings was examined using Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), potentiodynamic polarization , and electrochemical impedance techniques. An improved anticorrosive property was observed for the crosslinked coating compared to the untreated one, which could be due to the formation of the network barrier against water and corrosion ions such as chloride and oxygen. Furthermore, the cytocompatibility evaluation of different samples by CCK-8 assay suggested that the treatment of genipin was beneficial to the MC3T3-E1 cell proliferation . The study may provide sufficient evidence to show that more efficient LbL assembled multilayer coatings for improving anticorrosion and cytocompatibility would be the ones with crosslinking treatment.
研究领域:
材料科学
·
生物医学工程
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DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Wentao Shi,Yan Gao,Yiqing Wu,Jiaqi Sun,Bai Xu,Xiaojie Lu,Qing Wang
Here, we fabricated a hybrid nanoparticle composed of polydopamine nanoparticles (pNPs), alendronate (Al) and genipin (GP) for cranial bone defect repair. Al was crosslinked into pNPs via GP ( [email protected] ), after which hybrid nanoparticles were obtained. By embedding these [email protected] into the fibrin hydrogels, a multifunctional bone repair scaffold was fabricated ( [email protected] /Fg). The [email protected] /Fg exhibited three synergistic effects on the bone microenvironment: i) enhanced ectomesenchymal stem cell (EMSC) osteogenic differentiation by activating the piezo 1 channel; ii) inhibited the formation and function of osteoclasts related to the NF-κB signaling pathways; and iii) promoted M2 polarization and anti-inflammatory factor expression under normal and simulated inflammatory conditions. [email protected] /Fg ultimately promoted cranial bone defect regeneration in an SD rat model. This simple and low-cost technology provides a new approach to constructing an efficient delivery system and has desirable biological properties, providing a tissue-committed niche for the repair of bone defects.
研究领域:
生物材料
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组织工程
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再生医学
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纳米技术
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免疫学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Biofabrication
·
2017
·Vol. 9
·期 2
·025028
·
IF 5.24
Ruiying Chen,Xinjie Cai,Kena Ma,Yi Zhou,Yining Wang,Tao Jiang
Bone regeneration is a complicated process and includes a number of distinct and sequential stages of coordinated cellular actions under the regulation of multiple growth factors. Therefore, bone grafting materials in which growth factors can be incorporated and released in a programmed order in line with the bone tissue healing process may lead to desirable clinical outcomes. In the present study, a double-layered chitosan/gelatin/genipin (d-CSG/G) nanosphere coating is developed by using layer-by-layer electrophoretic deposition and genipin crosslinking. The surface morphology, physicochemical and mechanical properties of the coatings are explored. Cytochrome C is used as a therapeutic model protein and is successfully loaded on the inner and outer layers of the coating. The protein release can be controlled by the loading position, genipin concentration and thickness of the outer layer. Furthermore, the cell response to the coatings was evaluated. Real-time polymerase chain reactions, immunofluorescence staining and extracellular matrix mineralization assay confirmed that the functions of the loaded growth factor are fully preserved after fabrication. Overall, the d-CSG/G nanosphere coating could be a promising growth factor delivery system to promote bone tissue regeneration.
研究领域:
生物材料学
·
组织工程学
·
药物递送系统
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2024
·Vol. 35
·期 19
·1-13
·
IF 2.8
Ding Hongxin,Gu Yunqing,He Chendong,Ren Yun,Qiu Qianfeng,Hu Chaoxiang,Wu Denghao,Mou Jiegang,Wu ZhenXing
The poor actuation characteristics of current bionic artificial muscles limit their application in engineering practice. Chitosan and graphene are used as the main components to prepare bionic artificial muscles. The driving characteristic indexes of the bionic artificial muscles are proposed, including the output force, output displacement and the respective response speed, and the test platform is built. The mechanism of the influence of Multi-walled carbon nanotubes (MCNT) and Genipin on the actuation characteristics of the bionic artificial muscles is investigated. MCNT crosslinked with graphene enhances the electrical conductivity of the electrode layer, while Genipin crosslinked with chitosan improves the flexibility of the artificial muscles themselves. The bionic artificial muscle with co-addition of Genipin and MCNT showed improvement in output displacement, output force, and response speed. The failure mechanism of the bionic artificial muscle and the improvement mechanism of the additives were analyzed by both electron microscopy and water loss rate, and the changes in the microscopic surface of the electrode layer and the driving layer as well as the dissipation of the internal water were the main reasons for the failure.MCNT and Genipin improved the driving characteristics by enhancing the electrode capacity and accelerating the ion migration rate, respectively.
研究领域:
材料科学
·
生物工程
✓ 已验证
产品关联
DOI
高影响因子
RSC Advances
·
2015
·Vol. 6
·期 2
·1415-1421
·
IF 3.84
Wentao Hao,Liang Zhang,Xiaomin Wang,Jin Wang,Zhenhu Hu,Wen Yang
Tough and strong nacre-like composite films were fabricated from hyperbranched poly(amido amine) (HPAMAM) and clay nanosheets with the aid of genipin crosslinking. SEM observation and SAXS analysis indicated the genipin crosslinked HPAMAM/nanoclay composite films had a compact structure. However, DMA results showed that HPAMAM molecules still had excellent flexibility. FTIR analysis suggested that the HPAMAM molecules maintained their strong ability to form hydrogen bonds after crosslinking. Therefore, characteristics of sacrificial bonds were found. In addition, dual networks were thought to have been established inside the composite films, that is, a primary chemical network and a secondary physical network. Consequently, the genipin crosslinked HPAMAM/nanoclay composite films were shown to be tough and strong. The fracture toughness was as high as 5.03 MJ m−3 and the mechanical strength was about 152.9 MPa. Lastly, the good biocompatibility of all the components, including HPAMAM, nanoclay and genipin, give great opportunities for these composites to be widely applied in biomedical research.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2023
·
IF 6.1
Yulin Zhao,Haoyan Huang,Ning Lv,Chunyan Huang,Huili Chen,Han Xing,Chaorui Guo,Ning Li,Di Zhao,Xijing Chen,Yongjie Zhang
Genipin (GP), the reactive metabolite of geniposide (GE), is responsible for GE-induced hepatotoxicity. As a potential detoxification pathway, the inactivation of GP by glutathione S-transferases (GSTs) has not yet been characterized. In this study, the thiol-GSH conjugates of GP, M532-1 and M532-2 were first identified and the catalytic activities of GSTs were investigated both in vitro and in vivo. GSTA1-1 and GSTA4-4 showed high activity in the formation of both thiol-GSH conjugates, whereas GSTA4-4 specifically catalyzed M532-2 formation in vitro. The active GST isoforms protect against alkylation of N-acetylcysteine (NAC), a classic model nucleophile. GST inhibition attenuated M532-1 formation in rat bile, confirming the in vivo catalytic role of GSTs. In conclusion, this study demonstrated the inactivation of GP by GSTs and implied that interindividual variability of GSTs may be a risk factor for susceptibility to GE-induced hepatotoxicity.
研究领域:
药理学
·
毒理学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMOSPHERE
·
2024
·
IF 8.1
Mingi Ko,Taesoon Jang,Soyeong Yoon,Jooyoung Lee,Jin-Hyuk Choi,Jae-Woo Choi,Jeong-Ann Park
Emerging micropollutants, such as pharmaceuticals and microplastics (MPs), have become a pressing water environmental concern. The aim of this study is to synthesize chitosan sponges using graphene oxide (GO) and genipin (GP) for the removal of pharmaceuticals (diclofenac (DCF) and triclosan (TCS)) and MPs, verify their adsorption mechanisms, evaluate the effects of temperature, pH, and salinity on their adsorption capacities, and determine their reusability. The GO 5 /CS/GP sponge exhibited a macroporous nature (porosity = 95%, density = 32.6 mg/cm 3 ). GO and cross-linker GP enhanced the adsorption of DCF, TCS, and polystyrene (PS) MPs onto the CS sponges. The adsorption of DCF, TCS, and PS MPs involved multiple steps: surface diffusion and pore diffusion of the sponge. The adsorption isotherms demonstrated that Langmuir model was the most fitted well model to explain adsorption of TCS (q m = 7.08 mg/g) and PS MPs (q m = 7.42 mg/g) on GO 5 /CS/GP sponge, while Freundlich model suited for DCF adsorption (q m = 48.58 mg/g). DCF adsorption was thermodynamically spontaneous and endothermic; however, the adsorption of TCS and PS MPs was exothermic (283–313 K). The optimal pH was 5.5–7 due to the surface charge of the GO 5 /CS/GP sponge (pH zpc = 5.76) and ionization of DCF, TCS, and PS MPs. As the salinity increased, DCF removal efficiency drastically decreased due to the weakening of electrostatic interactions ; however, TCS removal efficiency remained stable because TCS adsorption was mainly caused by hydrophobic and π–π interactions rather than electrostatic interaction. The removal of PS MPs was enhanced by the electrostatic screening effects of high Na + ions. PS nanoplastics (average size = 26 nm) were removed by the GO 5 /CS/GP sponge at a rate of 73.0%, which was better than that of PS MPs (41.5%). In addition, the GO 5 /CS/GP sponge could be recycled over five adsorption–desorption cycles.
研究领域:
环境科学
·
材料科学
✓ 已验证
DOI
PubMed
高影响因子
Journal of Tissue Engineering and Regenerative Medicine
·
2020
·Vol. 14
·期 3
·497-509
·
IF 3.078
Yizhong Peng,Donghua Huang,Jinye Li,Sheng Liu,Xiangcheng Qing,Zengwu Shao
Biomaterial-based therapy that can restore annulus fibrosus (AF) function in early stage and promote endogenous repair of AF tissues is a promising approach for AF tissue repair. In this study, we established a genipin-crosslinked decellularized AF hydrogels (g-DAF-G) that are injectable and could manifest better in situ formability than noncrosslinked decellularized AF hydrogel, while preserving the capacity of directing differentiation of human bone mesenchymal stem cells (hBMSCs) towards AF cells. Hematoxylin and eosin staining, 4',6-diamidino-2-phenylindole staining, and so forth showed that the majority of cellular components were removed, whereas extracellular matrix and microstructure were largely preserved. The storage modulus increased from 465.5 ± 9.4 Pa to 3.29 ± 0.24 MPa after 0.02% genipin crosslinking of decellularized AF hydrogels (DAF-G) to form g-DAF-G. AF-specific genes (COL1A1, COL5A1, TNMD, IBSP, FBLN1) were significantly higher in DAF-G and g-DAF-G groups than that in control group after 21 days of culturing. g-DAF-G significantly restored nucleus pulposus water content and preserved intervertebral structure in vivo. Summarily, we produced a novel AF regeneration biomaterial, g-DAF-G, which exhibited well biocompatibility, great bioactivity, and much higher mechanical strength than DAF-G. This study will provide an easy and fast therapeutic alternative to repair AF injuries or tears.
研究领域:
组织工程
·
干细胞生物学
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生物材料科学
·
骨科
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Antioxidants
·
2022
·Vol. 12
·期 1
·74
·
IF 7.675
Wanying Liu,Ke Li,Menglin Zheng,Ling He,Tong Chen
The present study was conducted to evaluate the effect of genipin (GEN) on the microglia of diabetic cognitive impairment and explore its potential mechanism. Diabetic mice were induced by STZ/HFD, while GEN was intragastrically and intraventricularly treated. The human microglia cell HMC3 was induced by LPS/HG/PA. As a result, GEN attenuated diabetic symptoms and diabetic cognitive impairment-related behavior in novel object recognition, Morris water maze and passive avoidance tests. GEN inhibited M1 microglia polarization, lipid accumulation, oxidative stress and promoted mitochondrial fusion via FABP4/Mfn1. FABP4 overexpression, Mfn1 overexpression, selective FABP4 inhibitor BMS, and Mfn1 SiRNA were employed for investigating the mechanism. The inhibitory effect of GEN on ROS may be associated with NOX2 signaling and the translocation of p47phox/p67phox to the cell membrane. With the ROS scavenger NAC, it was proved that ROS participated in GEN-mediated inflammation and lipid accumulation. GEN inhibited the phosphorylation and nucleus translocation of NF-κB. GEN inhibited the ubiquitination of Mfn1, which was mediated by the E3 ligase Hrd1. GEN also enhanced microglia phagocytosis. Molecular docking predicted that GEN may interact with FABP4 by hydrogen bond at the S53 and R78 residues. In conclusion, GEN attenuated diabetic cognitive impairment by inhibiting inflammation, lipid accumulation and promoting mitochondrial fusion via FABP4/Mfn1 signaling.Keywords:diabetic cognitive impairment;genipin;microglia;FABP4;Mfn1
研究领域:
神经科学
·
药理学
·
糖尿病
·
细胞生物学
·
分子生物学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2022
·
IF 8.025
Rongtai Zuo,Jingjing Shi,Susu Jiang,Min Chu,Qiyang Wang,Lingchi Kong,Qinglin Kang,YaPing Guo,Junjie Guan
Diabetic skin disorders are lingering and refractory clinical diseases. In this study, a genipin-crosslinked porous chitosan fiber (CSF) hydrogel was fabricated to achieve rapid wound healing. By embedding clemastine fumarate (CF) in the CSF hydrogel pores, we synthesised a CSF/CF hydrogel for the treatment of diabetic wounds. The microstructure, chemical elements, spectral variation, mechanical properties, swelling ratios, degradability, and toxicity of the CSF/CF hydrogels were studied. Compared with the typical CS power hydrogel, the porous CSF hydrogel crosslinked with genipin possesses a stable structure and improved physicochemical properties. Moreover, CF was slowly released from the CSF hydrogel. Molecular simulation also showed that CF was evenly embedded inside the cavity formed by the novel CSF hydrogel. The results suggested that CF can resist damage from high glucose levels and promote proliferation, tube formation, and migration of endothelial cells (ECs) and fibroblasts. The CSF/CF hydrogel promoted wound healing in a rat model. Mechanistically, the beneficial effect of CF on wound healing may be related to activation of the MEK/ERK and PI3K/Akt signalling pathways. In conclusion, genipin-crosslinked CSF/CF hydrogel can accelerate wound healing and may be an effective therapeutic method for treating diabetic skin lesions.
研究领域:
生物医学工程
·
药剂学
·
伤口愈合
·
糖尿病
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）