RSC Advances
·
2025
·Vol. 15
·期 46
·38585-38596
·
IF 4.6
Shukai Sun,Yue Zhai,Qingwu Tian,Peng Zhao,Hong Cheng,Deng Pan,Junna Sui,Yusun Zhou,Chao Xuan,Tingting Zhou
Magnetic molecularly imprinted polymers (MMIPs) were synthesized using magnetic Fe3O4@SiO2 as a carrier for the selective recognition and adsorption of doxorubicin (DOX) and epirubicin (EPI) in plasma. Then, the method was combined with high-performance liquid chromatography-ultraviolet detection (HPLC-UV) to realize rapid and accurate detection of DOX and EPI concentrations in plasma. The structures of the prepared MMIPs were characterized by X-ray diffraction, transmission electron microscopy, X-ray photoelectron spectroscopy, and magnetic saturation. As observed, the prepared MMIPs had a uniform appearance and excellent magnetic properties. Then, the adsorption performance of MMIPs was verified through static, dynamic, and selective adsorption tests. MMIPs were found to have high adsorption capacity (8.36 and 8.19 mg g−1), high selectivity (imprinting factors of 34.83 and 8.03), and rapid adsorption for DOX and EPI, respectively. Next, HPLC-UV was used for quantitative analysis. The prepared method had a good linear relationship (0.0001–0.5 mg mL−1 for DOX and 0.0002–0.5 mg mL−1 for EPI) and low detection limits (0.04613 ng mL−1 for DOX and 0.08969 ng mL−1 for EPI). The combination of magnetic solid-phase extraction and HPLC-UV was a highly specific and sensitive method for determining DOX and EPI concentrations in plasma.
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PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
·
2023
·
IF 3
Hong Yang,Jing Wang,Wenming Cao
Tetramethylpyrazine (TMP) is an important bioactive compound in vinegars, contributing to their health-enhancing attributes. It serves as a crucial benchmark for the assessment of vinegar quality. Unfortunately, inaccuracies have arisen due to incomplete extraction techniques and the use of an inappropriate standard substance. These challenges have significantly curtailed comprehensive exploration into the underlying TMP formation mechanisms, impeding advancements within prevailing benchmarks and methodologies governing vinegar products. To address these challenges, several critical parameters, encompassing pH, solvent type, centrifugal force, extraction times and reference materials were investigated and optimized. The TMP content was determined by adjusting the pH to 9 using a sodium hydroxide solution, followed by extraction with ethyl acetate and subsequent re-extraction of the ethyl acetate layer with 0.2 mol/L HCl. A high-performance liquid chromatography method with an ultraviolet detector (UV) was developed and validated. This method demonstrated superior sensitivity compared to existing methods, with a limit of detection (LOD) of 0.0237 μg/g, limit of quantification (LOQ) of 0.0829 μg/g, method limit of detection (MLOD) of 0.10 μg/g and method limit of quantitation (MLOQ) of 0.25 μg/g. The modified method exhibited excellent linearity for TMP in the range of 0.1–118.4 μg/mL, with a good correlation coefficient (R 2 > 0.999). The recovery rate of TMP in vinegar products ranged from 82.4 to 96.2%. Consequently, the proposed method exhibits substantial promise for systematic inquiry into TMP formation mechanisms and for ensuring consistent quality control during the production of premium-grade vinegars.
研究领域:
食品科学
·
分析化学
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PubMed
高影响因子
MOLECULES
·
2022
·Vol. 27
·期 22
·7683
·
IF 4.927
Meng-Fei Guo,Huan-Huan Zhang,Ping Zhong,Jin-Di Xu,Shan-Shan Zhou,Fang Long,Ming Kong,Qian Mao,Song-Lin Li
Cicadae Periostracum (CP) is a traditional Chinese medicinal herb derived from the slough that is molted from the nymph of the insectCryptotympana pustulataFabricius. Washing with water to remove residual silt is a primary processing method of CP that is recommended by the Chinese Pharmacopoeia, but how washing methods affect the quality and bioactivity of CP is unknown. In this study, the quality and bioactivity of non-washed CP (CP-NW), post-molting-washed CP (CP-WAT), and pre-molting-washed CP (CP-WBT) were comparatively investigated. The quality of these CP samples was evaluated in terms of the UPLC-QTOF-MS/MS-based chemical profiling and semi-quantification of 39N-acetyldopamine oligomers (belonging to six chemical types), the HPLC-UV-based quantification of 17 amino acids, the ICP-MS-based quantification of four heavy metals, and the contents of ash; the bioactivities of the samples were compared regarding their anti-oxidant and anti-inflammatory activities. It was found that, compared with CP-NW, both CP-WBT and CP-WAT had significantly lower contents of ash and heavy metals. Moreover, compared with CP-WAT, CP-WBT contained lower levels of total ash, acid-insoluble ash, and heavy metals and higher contents ofN-acetyldopamine oligomers and amino acids. It also had enhanced anti-oxidant and anti-inflammatory activities. A Spearman’s correlation analysis found that the contents ofN-acetyldopamine oligomers and free amino acids were positively correlated with the anti-oxidant/-inflammatory activities of CP. All these results suggest that pre-molting washing can not only remove the residual silt but can also avoid the loss of the bioactive components and assure higher bioactivities. It is concluded that pre-molting washing could enhance the quality and bioactivity of CP and should be a superior alternative method for the primary processing of qualified CP.Keywords:Cicadae Periostracum;primary processing;pre/post-molting washing;quality;bioactivity
研究领域:
药用植物学
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药物化学
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中药学
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分析化学
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高影响因子
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
·
2016
·
IF 0.669
Yu Wang,Jing Yang,Yanfang Guo,Haipeng Wang,Yalan Liu,Huiming Huang,Baogang Xie
The determination of alendronate (ALE) in biofluids using a low-cost instrument is potentially useful in preclinical pharmacokinetic studies. This study developed and validated a high-performance liquid chromatography with ultraviolet method for ALE determination in rat plasma using precolumn derivatization with phenyl isothiocyanate (PITC). Inhibiting compounds in the samples were first eliminated using solid-phase extraction. ALE in the sample was subsequently allowed to react with PITC to form a phenylthiocarbamoyl derivative for further analysis. The assay was linear within the concentration range of 0.29–25.0 µg/mL. The precision and accuracy were less than 3.9% and 98.0 ± 3.9%, respectively. The limits of detection and quantification were 0.08 and 0.20 µg/mL, respectively. The method was successfully used to evaluate the pharmacokinetic parameters of ALE in rats following a single oral administration (30.0 mg/kg). The results show that the peak plasma ALE concentration is 0.69 ± 0.18 µg/mL. The area under the plasma concentration–time curve value of ALE was 2.14 ± 0.68 µg/mL hr. This method can suitably evaluate the bioavailabilities of different ALE dosage forms in preclinical pharmacokinetic studies.
研究领域:
药物分析
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临床药理学
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高影响因子
JOURNAL OF CHROMATOGRAPHIC SCIENCE
·
2017
·Vol. 55
·期 6
·662-668
·
IF 0.984
Ma Xiaowei,Feng Fan,Yang Yang,Dang Xueping,Huang Jianlin,Chen Huaixia
Fe3O4@MIL-100 (MIL, Material Institut Lavoisier) core–shell magnetic microspheres were prepared and applied as the sorbent for the magnetic solid phase extraction (MSPE) of N,N-diethyl-m-toluamide (DEET) in baby toilet water for the first time. The synthesized magnetic metal–organic frameworks were characterized by transmission electron microscope, infrared spectroscopy and thermogravimetric analysis. The functionalized magnetic microparticles showed excellent dispersibility in aqueous solution. The MSPE conditions were investigated in detail. Under the optimized conditions, an MSPE–high performance liquid chromatography method for the determination of DEET was developed. The method was linear in the concentration range from 5 to 500 μg L−1 for DEET in baby toilet water and good linearity (r2 > 0.9998) was obtained for the calibration curve. The limit of detection was 1.5 μg L−1. Both the intra-day and inter-day precisions (relative standard deviations) were <3%.
研究领域:
分析化学
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环境科学
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材料科学
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PubMed
高影响因子
TALANTA
·
2025
·
IF 6.1
Manyan Qiu,Aiai Nie,Jinghang Zhang,Miao Zheng,Ning Guan,Sharuna De,Xiaoyan Pei,Xinyan Yang,Yujun Jiang,Qianyu Zhao,Chaoxin Man
The effective separation of fat-soluble vitamins from complex matrices prior to instrumental analysis remains a significant challenge. Herein, a novel magnetic covalent organic framework (Fe 3 O 4 @tetrakis(4-aminophenyl)methane (TAM)-2,6-dihydroxynaphthalene-1,5-dicarbaldehyde (DHNDA), Fe 3 O 4 @TAM-DHNDA) was successfully designed and prepared for the efficient separation and enrichment of three fat-soluble vitamins in dairy products. The maximal adsorption efficiency of Fe 3 O 4 @TAM-DHNDA to fat-soluble vitamin was as high as 26.94 mg/g. Moreover, the adsorption mechanism of Fe 3 O 4 @TAM-DHNDA for V A , V D , and V E was revealed by molecular simulation techniques. Van der Waals forces and hydrogen bonds were the main interactions between Fe 3 O 4 @TAM-DHNDA and fat-soluble vitamins. Importantly, the magnetic solid phase extraction (MSPE) method based on Fe 3 O 4 @TAM-DHNDA could be used as an effective sample pretreatment technology for separation and enrichment of fat-soluble vitamins from complex matrices prior to instrumental analysis. When coupled with high-performance liquid chromatography-ultraviolet detector (HPLC-UV), the proposed method enables the efficient detection of fat-soluble vitamins in dairy products. The limit of detection (S/N = 3) and limit of quantification (S/N = 10) were achieved at 20–50 ng/mL and 55–150 ng/mL, respectively. Good linearity (R 2 > 0.9998) was obtained within the range of 2–100 μg/mL. The average recoveries of spiked milk samples were determined to be 83%–101% and the relative standard deviation (RSD) was 0.83% to 3.46% ( n =3). This work provides novel insights into the development of novel magnetic adsorbents and the separation and enrichment of fat-soluble vitamins in complex matrices.
研究领域:
分析化学
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食品科学
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材料科学
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PubMed
高影响因子
Analytical Methods
·
2018
·Vol. 10
·期 15
·1733-1739
·
IF 2.073
Weixia Li,Ning Chen,Yonggang Zhao,Weiqiang Guo,Nadeem Muhammd,Yan Zhu,Zhongping Huang
N-Nitrosamines (NAs) are potent carcinogens and have been found in food products. Herein we report a simple tandem liquid-phase extraction (TLPE) method coupled with high-performance liquid chromatography (HPLC) for the determination of trace N-nitrosamines (NAs) in food products. The TLPE method is based upon the tandem implementation of pressurized hot water extraction (PHWE) and dispersive liquid–liquid microextraction (DLLME). The target analytes were first extracted with pressurized hot water, and then concentrated into an organic phase (n-octanol) in the DLLME procedure. The TLPE-HPLC system allowed the analytes to be extracted, concentrated and analyzed in a single step with minimal manipulation of the sample preparation, with an overall analysis time of 25 min. The optimized method showed good linearity (r2 ≥ 0.981) and satisfactory recoveries (74–117%) with good repeatability of the extractions (RSDs below 12.8%, n = 5). The limits of detection (LODs) for all nitrosamines in salted duck eggs were in the range of 0.08–0.55 μg kg−1.
研究领域:
分析化学
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食品科学
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环境科学
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高影响因子
Separation Science Plus
·
2018
·Vol. 1
·期 5
·367-373
·
IF 0
Xuemei Wang,Huan Wang,Pengfei Huang,Xiaomin Ma,Xinzhen Du,Xiaoquan Lu
A specific, precise and accurate high-performance liquid chromatography method with ultraviolet detection was established for the simultaneous determination of eight insecticides in fruit samples. Samples were extracted with acetone and cleaned using the quick, easy, cheap, effective, rugged, and safe technique. Under the optimized conditions, wide linearity ranges (5.0–2000 μg kg −1 ) were obtained for the eight insecticides and the correlation coefficients ( r ) ranged from 0.9985 to 0.9994. The limit of detection and limit of quantification were 0.27–1.85 and 0.90–6.18 μg kg −1 , respectively, and acceptable reproducibility (RSD < 6.6% for intra-day, < 9.2% for inter-day) were achieved. The recovery rates for all insecticides at two spiked levels of 20 and 50 μg kg −1 in fruits samples ranged from 85.9 to 110.4%, and the relative standard deviations were in the range of 3.3–8.9%. The results demonstrated clearly that the approach developed provides reliable, simple, rapid and environmentally friendly quantification and identification of eight insecticides in fruits. These results not only gave insights about the insecticides but also contributed to environmental protection and food safety.
研究领域:
食品科学
·
分析化学
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FOOD CHEMISTRY
·
2020
·
IF 6.306
Qiuhua Wu,Yuhong Song,Qianqian Wang,Weihua Liu,Lin Hao,Zhi Wang,Chun Wang
A magnetic o -hydroxyazobenzene (M-HAzo) porous organic polymer was facilely prepared by a green azo coupling reaction in aqueous solution. The prepared M-HAzo was applied as a new adsorbent for the first time to pre-concentrate phthalate esters (PAEs) from plastic bottled juice, followed by their determination with high performance liquid chromatography-ultraviolet detection. The effects of various parameters, i.e., the mass ratio of the Fe 3 O 4 @SiO 2 to HAzo, extraction time, ionic strength, pH of the sample, desorption conditions were optimized. Under the optimized conditions, the M-HAzo based method exhibited good performance in terms of linear range (0.3–50.0 μg L −1 ), detection limit (0.08–0.50 μg L −1 ), accuracy (recovery of 78.0–115.0%) and repeatability (relative standard deviation of 2.9–7.8%). This work provides a sensitive method for analysis of PAEs at trace levels in drinks, which is featured with high sensitivity, simple operation and environmentally-friendly merit and will have a promising potential in analysis of other organic pollutants.
研究领域:
分析化学
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环境科学
·
食品科学
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PubMed
高影响因子
BIOINORGANIC CHEMISTRY AND APPLICATIONS
·
2022
·
IF 4.724
Yuqin Yang,Junjie Miao,Zhendong Yin,Weili Hao,Hongmei Shi,Ling Ma,Tiesheng Shi
Efficient extractions of trace antibiotic residues in the environment are a key factor for accurate quantification of the residues. A new nanoporous material, namely, magnetically covalent organic polymer (MCOP, Fe3O4@SiO2-NH2-COP) was synthesized in this work and was used for magnetic solid-phase extraction (MSPE). The combination of MSPE with high-performance liquid chromatography separation together with ultraviolet detection (HPLC-UV) was established as an effective method for the determination of four sulfonamide (SA) residues in surface water surrounding a cattle farm. The synthesized magnetic material was characterized by SEM, TEM, FT-IR, magnetic properties measurement system (MPMS), and nitrogen gas porosimetry. The material possessed many attractive features, such as a unique microporous structure, a larger specific surface area (137.93 m2·g−1) than bare Fe3O4 (24.84 m2·g−1), high saturation magnetization (50.5 emu·g−1), open adsorption sites, and high stability. The influencing parameters, including pH, the used amount of MCOPs, the type of eluent, adsorption solution, and desorption time, were optimized. Under the optimized conditions, the method conferred good linearity ranges (R2 ≥ 0.9990), low detection limits (S/N = 3, LOD, 0.10–0.25 μg·L−1), and satisfactory recoveries (79.7% to 92.2%). The enrichment factor (EF) for the four SAs was 34.13–38.86. The relative standard deviations of intraday (n = 5) and of interday (n = 3) were less than 4.8% and 8.9%, respectively. The equilibria between extraction and desorption for SAs could be reached within 150 s. The proposed method was sensitive and convenient for detecting SA residues in complex environmental matrices, and the successful application of the new MCOPs as an adsorbent was demonstrated.
研究领域:
环境科学
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分析化学
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材料科学
·
水质
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PubMed
高影响因子
Scientific Reports
·
2025
·Vol. 15
·期 1
·1-14
·
IF 3.9
Zhou Lei,Zhang Mengting,Sun Huinan,Duan Liyuan,Meng Qingwei
This study establishes the first reversed-phase HPLC-UV method for simultaneous baseline separation of 12 structurally diverse water-soluble vitamins (WSVs) within 25 min. The eco-friendly approach utilizes a methanol–potassium dihydrogen phosphate (pH 4.85) gradient on a ChromCore C18 column (250 × 4.6 mm, 5 μm) with dual-wavelength detection (210/266 nm), eliminating environmentally detrimental ion-pairing reagents. Rigorous validation per ICH Q2(R1) guidelines confirmed excellent linearity (R² > 0.999), precision (RSD < 2.1%), accuracy (spike recoveries: 93.9–106.8%), and sensitivity (LODs: 0.01–0.78 µg/mL). The method resolved key analytical challenges including polarity disparities, matrix interference, and vitamin instability. Successful quantification of endogenous vitamins in DMEM medium showed ≤ 5.1% deviation from certified values for 11/12 analytes. Applied to five commercial MDCK cell culture media, the method revealed significant inter-product variability in vitamin composition – notably universal under-supplementation of riboflavin (0.576–0.741 µg/mL, 10× lower than other vitamins) and inconsistent biotin/adenine supplementation. This robust, cost-effective platform provides biomanufacturers with a powerful quality control tool for optimizing vitamin supplementation in vaccine production systems, addressing a critical gap in cell culture analytics.
研究领域:
分析化学
·
生物化学
·
生物技术
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药学
·
细胞生物学
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PubMed
高影响因子
Food Quality and Safety
·
2026
·
IF 4.9
Li Xiangyu,Liu Yingfei,Luo Yanbo,Jiang Xingyi,Chai Ying,Qiao Yuemei,Zhang Hongfei,Zhu Fengpeng,Pang Yongqiang,Chen Di
Nicotinamide mononucleotide (NMN) has gained significant attention as a promising anti-aging and health-promoting supplement, driving the development of efficient and sustainable production methods. Among these methods, enzymatic synthesis has emerged as a highly specific and environmentally friendly approach. This study presents a comprehensive evaluation of NMN synthesized via two key enzymatic pathways—nicotinamide riboside kinase (NRK)-mediated and nicotinamide phosphoribosyltransferase (NAMPT)-mediated routes—along with commercially available raw NMN materials. Key quality attributes, including purity, content, moisture levels, thermal stability, and biological activity, were systematically assessed. Results show that NAMPT-synthesized NMN consistently achieves 100% purity, matching pharmaceutical-grade standards, while NRK-synthesized NMN displays batch-dependent variability, with purity ranging from 92.13% to 96.19%. Stability analysis reveals that NAMPT-derived NMN maintains high thermal stability, whereas NRK-derived NMN undergoes significant physical and chemical degradation at elevated temperatures, with nicotinamide as the primary degradation product. Additionally, bioactivity testing demonstrated that NAMPT-derived NMN retains significantly higher biological activity compared with NRK-synthesized and commercial NMN. These findings provide essential data for optimizing NMN production processes and support the establishment of industry-wide quality control standards.
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高影响因子
危险品化学品经营许可证（带存储）