ANALYST
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2019
·Vol. 144
·期 6
·1948-1954
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IF 4.019
Xin Gong,Xiao Li,Taiping Qing,Peng Zhang,Bo Feng
Tetracycline (TC) is widely used to treat bacterial infections in humans and animals due to its low price and good antibacterial properties. The abuse of tetracycline has led to TC residues in daily food that could seriously affect human health. Thus, it is imperative to develop highly sensitive and selective methods for TC detection. In this work, we developed a colorimetric method for TC detection based on an enzyme-linked aptamer assay (ELAA) with multivalent HRP-mimicking DNAzyme. An aptamer was used as an alternative recognition element in the enzyme-linked immunosorbent assay (ELISA). Multivalent HRP-mimicking DNAzyme, assembled via hybridization chain reactions (HCR), was used for catalytic substrate color rendering in ELAA. The multivalent HRP-mimicking DNAzyme exhibited enhanced catalytic capacity and improved the detection sensitivity greatly. The limit of detection was 8.1 × 10−2 ng mL−1 with a linear range from 1.0 × 10−2 ng mL−1 to 1.0 × 104 ng mL−1 toward TC in buffer. To challenge the practical application capability of this strategy, the detection of TC in milk samples was also investigated and showed similar linear relationships. Due to the introduction of an aptamer, this ELAA strategy shows high selectivity towards TC and has potential for the detection of a wide spectrum of analytes.
研究领域:
分析化学
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食品安全
·
生物技术
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JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
·
2017
·Vol. 17
·期 7
·5109-5114
·
IF 1.483
Fang Cheng,Cao Liangli,Chen Zhencheng
A disposable electrochemical immunosensor for detection of human alpha-fetoprotein (AFP) has been developed in this study, using monoclonal antibody against AFP and horseradish peroxidase co-modified colloidal gold nanoparticles (Ab-Au-HRP) as trace label and screen-printed carbon electrodes
(SPCEs) as signal transducers. The immunosensor was based on a sandwich immunoassay scheme in which the nanoprobe Ab-Au-HRP was dispensed on the polyester membrane as conjugate pad. The other monoclonal antibody against AFP, as capture antibody, was immobilized onto the nitrocellulose membrane
which was mounted on the carbon surface of the screen-printed electrodes. An absorbent paper was mounted downstream to draw the sample to successively flow through the conjugate, capture and absorbent pads. The resulting sandwich immune reaction was evaluated by electrochemical detection,
through addition of a single-component substrate for HRP. Under optimum conditions, the current value was linearly increased with an increased AFP concentration from 0.5 to 350 ng/mL, at a relatively low detection limit of 0.25 ng/mL at 3 sigma. Excellent analytical performance, fabrication
reproducibility and operational stability for the proposed immunosensor indicated its promising application in clinical diagnostics and self-exams.
研究领域:
生物医学工程
·
临床诊断学
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2022
·
IF 8.025
Biaobiao Yan,Shuaibing Huang,Yiwen Ren,Man Zhou,Yuanyuan Yu,Bo Xu,Li Cui,Qiang Wang,Ping Wang
MXene-based functional textiles have been widely studied and applied in many fields. However, the service stability of MXene combined with textile substrates in the environment is far from ideal, which makes its practical application a great challenge. Here we introduced gallic acid (GA), as natural reactive polyphenol compound to silk fibers through enzymatic polymerization, which significantly improved the durability of its conductivity. The small molecules of GA can covalently bind to the titanium atoms on the MXene nanosheets, and the tyrosine residues from silk fibroins can be enzymatically oxidized by horseradish peroxidase (HRP) and further coupled with GA simultaneously, thus forming a covalent cross-linked network on the fiber surfaces. Furthermore, the durable MXene-based textile was used to manufacture smart dual-driven thermal devices with temperature monitoring, which can judge the real-time temperature during heating by changes in its apparent color. More importantly, the textile with smart temperature visualization also offers good EMI shielding and superior UV resistance, while retaining its inherent moisture-wicking, breathable and softness. The present work provides a new insight for the preparation of MXene-based multifunctional textile, and the smart visualization of dual-driven heating shows promising applications in practical personal thermal management.
研究领域:
材料科学
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生物化学
·
纺织工程
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BIOSENSORS & BIOELECTRONICS
·
2019
·
IF 9.518
Yun Zhang,Wenqing Tan,Yang Zhang,Huili Mao,Shuyou Shi,Liangwei Duan,Hui Wang,Junping Yu
To develop a specific method for the detection of S. aureus , chicken anti-protein A IgY was adopted for specifically capturing S. aureus , depending on the specific recognition of staphylococcal protein A (SPA) by chicken anti-protein A IgY, which can eliminate the interference from protein G-producing Streptococcus . HRP labeled IgG , Fc region of which has a high affinity towards SPA, was paired with IgY for the colorimeter analysis of the system. By optimizing the system, a super-low detection limit of 11 CFU of S. aureus in 100 μL PBS without enrichment, with a linear range from 5.0 × 10 2 CFU mL −1 to 5.0 × 10 4 CFU mL −1 was obtained. The entire assay was accomplished in less than 90 min and no cross-reactivity with the other tested bacterial species was observed. Moreover, the developed assay has been applied for the detection of S. aureus in three different types of real samples (sodium chloride injection, apple juice and human urine) with satisfactory results. To the best of our knowledge, it is the first time to report using chicken anti-protein A IgY and any IgG to detect S. aureus based on the dual-recognition mode of SPA. The novel method opened up a way for monitoring S. aureus in food samples with high sensitivity, specificity and simple operation.
研究领域:
微生物学
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分析化学
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食品安全
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Materials
·
2021
·Vol. 14
·期 22
·6901
·
IF 3.623
Yanru Ren,Xiaoyan Qin,Mike Barbeck,Yi Hou,Haijun Xu,Luo Liu,Chaoyong Liu
Infection-related titanium implant failure rates remain exceedingly high in the clinic. Functional surface coating represents a very promising strategy to improve the antibacterial and bioactive properties of titanium alloy implants. Here, we describe a novel bioactive surface coating that consists of a mussel-inspired carboxymethyl chitosan hydrogel loaded with silver nanoparticles (AgNPs) to enhance the bioactive properties of the titanium alloy. The preparation of hydrogel is based on gallic acid grafted carboxymethyl chitosan (CMCS-GA) catalyzed by DMTMM (4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride). To build a firm bonding between the hydrogel and titanium alloy plate, a polydopamine layer was introduced onto the surface of the titanium alloy. With HRP/H2O2catalysis, CMCS-GA can simply form a firm gel layer on the titanium alloy plate through the catechol groups. The surface properties of titanium alloy were characterized by scanning electron microscope (SEM), X-ray photoelectron spectroscopy (XPS), and water contact angle. Silver nanoparticles were loaded into the gel layer by in situ reduction to enhance the antibacterial properties. In vitro antibacterial and cell viability experiments showed that the AgNPs-loaded Ti-gel possesses excellent antibacterial properties and did not affect the proliferation of rabbit mesenchymal stem cells (MSCs).Keywords:carboxymethyl chitosan;gallic acid;titanium alloy;DMTMM;HRP/H2O2;catechol group;hydrogel coating;antibacterial
研究领域:
材料科学
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生物材料
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生物医学工程
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MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Yalong Cheng,Juan Qiao,Shidi Zhang,Li Qi
Metal-organic frameworks (MOFs) have attracted extensively interests for enzyme immobilization. However, their rigid structures remain a great challenge in improvement the catalytic performance of the carriers due to no nanoconfinement. Herein, carriers based on rigid UiO-66-NH 2 (U) modified with soft smart polymer were designed by surface engineering. A thermo-responsive polymer, poly(N-isopropylacrylamide) (PNP), was synthesized through an in-situ polymerization strategy, which was employed as a “soft tent” on the surface of rigid U. Glucose oxidase (GOx)-horseradish peroxidase (HRP) was chemically bonded on PNP-U using glutaraldehyde as a linker. Benefiting from the nanoconfinement via variation temperatures, PNP-U@GOx-HRP carriers exhibited 2.01-fold catalytic activity ( K cat value) at 37 °C higher than that at 25 °C. Furthermore, a colorimetric method for analysis of glucose in grape samples was developed. This study offers a strategy for construction of “soft tent” smart polymer-MOFs@enzyme carriers, which provides a pivotal nanoconfinement for boosting their catalytic performance, and broadens their applications in bio-molecules sensing.
研究领域:
生物化学
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材料科学
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分析化学
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BIOSENSORS & BIOELECTRONICS
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2015
·
IF 6.409
Yan Zhao,Yiqun Zheng,Rongmei Kong,Lian Xia,Fengli Qu
We report an ultrasensitive electrochemical immunosensor designed for the detection of protein biomarkers using horseradish peroxidase (HRP)-loaded silica-poly(acrylic acid) brushes (SiO 2 -SPAABs) as labels. HRP could be efficiently and stably accommodated in the three-dimensional architecture of the SiO 2 -SPAABs and the SiO 2 -SPAABs-HRP exhibited high catalytic performance towards o -phenylenediamine (OPD) oxidation in the presence of H 2 O 2 , which resulted in significant differential pulse voltammetric (DPV) response change and color change. Using human IgG (HIgG) as a model analyte, a sandwich-type immunosensor was constructed. In particular, graphene oxide (GO) and SiO 2 -SPAABs-HRP were used to immobilize capture antibody (Ab 1 ) and bind a layer of detection antibody (Ab 2 ), respectively. The current biosensor exhibited a good linear response of HIgG from 100 pg/mL to 100 μg/mL with a detection limit of 50 pg/mL ( S / N =5). The sensitivity was 6.70-fold higher than the conventional enzyme-linked immunosorbent assays. The immunosensor results were validated through the detection of HIgG in serum samples.
研究领域:
生物医学工程
·
分析化学
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Polymers
·
2018
·Vol. 10
·期 5
·557
·
IF 2.935
Jinqiu Yang,Shenzhou Lu,Tieling Xing,Guoqiang Chen
Atom transfer radical polymerization (ATRP) is a “living”/controlled radical polymerization, which is also used for surface grafting of various materials including textiles. However, the commonly used metal complex catalyst, CuBr, is mildly toxic and results in unwanted color for textiles. In order to replace the transition metal catalyst of surface-initiated ATRP, the possibility of HRP biocatalyst was investigated in this work. 2-hydroxypropyl methacrylate (HPMA) was grafted onto the surface of silk fabric using the horseradish peroxidase (HRP) biocatalyzed ATRP method, which is used to improve the crease resistance of silk fabric. The structure of grafted silk fabric was characterized by Fourier transform infrared spectrum, X-ray photoelectron spectroscopy, thermogravimetic analysis, and scanning electron microscopy. The results showed that HPMA was successfully grafted onto silk fabric. Compared with the control silk sample, the wrinkle recovery property of grafted silk fabric was greatly improved, especially the wet crease recovery property. However, the whiteness, breaking strength, and moisture regain of grafted silk fabric decreased somewhat. The present work provides a novel, biocatalyzed, environmentally friendly ATRP method to obtain functional silk fabric, which is favorable for clothing application and has potential for medical materials.Keywords:horseradish peroxidase;atom transfer radical polymerization;bio-catalysis;silk
研究领域:
材料科学
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纺织工程
·
生物化学
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2023
·
IF 4.3
Zou Rong,Cao Lei,Wu Nan,Chang Gang,Li Li,Xiao Lu,Yan Huiling,Li Hongjie,Wang Ping,Bao Ting,Zhang Xiuhua,Wang Shengfu,Wang Yaping,He Hanping
Alpha-fetoprotein (AFP) is inextricably linked to various diseases, including liver cancer. Thus, detecting the content of AFP in biology has great significance in diagnosis, treatment, and intervention. Motivated by the urgent need for affordable and convenient electronic sensors in the analysis and detection of aqueous biological samples, we combined the solution-gated graphene transistor (SGGT) with the catalytic reaction of enzyme nanoprobes (HRP-AuNPs-Ab2) to accurately sense AFP. The SGGT immunosensor demonstrated high specificity and stability, excellent selectivity, and excessive linearity over a range of 4 ng/mL to 500 ng/mL, with the lower detection limit down to 1.03 ng/mL. Finally, clinical samples were successfully detected by the SGGT immunosensor, and the results were consistent with chemiluminescence methods that are popular in hospitals for detecting AFP. Notably, the SGGT immunosensor is also recyclable, so it has excellent potential for use in high-throughput detection. Graphical abstract
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ANALYTICAL CHEMISTRY
·
2018
·
IF 6.042
Zhonghui Chen,Chaoqun Chen,Huawei Huang,Fang Luo,Longhua Guo,Lan Zhang,Zhenyu Lin,Guonan Chen
Hydrogen sulfide (H2S) is important for normal neural functions, which involves protecting neurons from oxidative stress and neuronal transmission modulation in brain. The detection of H2S is significant for revealing its role in the diagnosis of various disease. In this study, a novel multicolor colorimetric assay based on the etching of gold nanorods (Au NRs) is proposed to evaluate H2S level with the naked eye. This measurement relies on the catalytic oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) via horseradish peroxidase (HRP) to produce TMB2+, which could etch the Au NRs quickly and accompany with a distinct color change. The vivid colors can be easily distinguished with the naked eye without any sophisticated instruments. The presence of H2S can cause the deactivation of HRP, which affects the amount of TMB2+ produced and consequently affects the color changing of the system. Based on this mechanism, a simple but sensitive multicolor colorimetric assay is developed for H2S detection with a linear range of 0.05–50 μM. The proposed method is demonstrated for monitoring extracellular H2S in rat brain coupled with microdialysate.
研究领域:
分析化学
·
神经科学
·
生物医学工程
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COLLOIDS AND SURFACES B-BIOINTERFACES
·
2021
·
IF 5.268
Yuanyuan Liu,Yihan Liu,Linyan Gu,Juan Han,Wenjing Zhi,Yun Wang,Lei Wang
Encapsulating enzyme within MOF (enzyme-MOF) gives rise to new opportunity to improve the fragility of enzyme, but practical application of enzyme-MOF composite is far from being realized. The development of a novel enzyme-MOF composite system should simultaneously guarantee the enhanced activity and controllably complete recycling, and only in this way can we efficiently and economically utilize the enzyme-MOF composite. Herein, we addressed all these fundamental limitations of current enzyme-MOF composite by establishing aptamer-functionalized enzyme-MOF composite (HRP-ZIF-8@P1). HRP-ZIF-8@P1 relied on automatic structure switch of aptamer-target binding and aptamer-cDNA (complementary DNA) hybridization, achieving effectiveness in self-enriching substrate around HRP-ZIF-8@P1 to boost enzymatic activity first, subsequently hybridizing spontaneously with magnetically controllable cDNA sequence (Fe 3 O 4 @P3) to completely recover the HRP-ZIF-8@P1, where preferentially capturing substrate could further induce the release of the hybridized HRP-ZIF-8@P1 for automatically starting the cyclic enzyme catalysis. A 5.6-fold enhancement in the catalytic efficiency for BPA degradation was endowed, and 94.7% catalytic activity was retained for 8 consecutive degradations of BPA, both of which were even more significant than HRP-ZIF-8. Additionally, remarkable stability of HRP-ZIF-8@P1 was afforded by dual-layer protection of ZIF-8 and P1 in denaturing conditions. Taking the possibility of discovering an aptamer for any target into account, the aptamer-functionalized enzyme-MOF composites provide a generic and simple guide for simultaneously boosting enzymatic activity and controllably full recycling the enzyme-MOF systems, accelerating their commercial utilizations.
研究领域:
材料科学
·
生物技术
·
环境化学
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FOOD CHEMISTRY
·
2026
·
IF 10.4
Junxiang Zhang,Zixin Yuan,Yingli Guo,Baomiao Ding,Xingping Zhang,Xinjun Xu,Hualin Yang
The new probe PP(Fe)@HRP with high catalytic activity was used in immunoassay. • The proposed immunoassay showed excellent sensitivity for detecting OTA. • The proposed immunoassay had fluorescence and absorbance dual-mode. Ochratoxin A (OTA) is a mycotoxin with carcinogenic properties. Thus, it is essential to establish a sensitive method for detecting OTA. In this work, a colorimetric and fluorescence dual-mode OTA immunoassay was established based on PP(Fe)@HRP probe. The PP(Fe)@HRP probe consists of PCN-224(Fe), PtNPs and HRP, all of which possess peroxidase activity. When combined to form PP(Fe)@HRP, the peroxidase activity increased significantly. Then, the new probe was used to oxidize TMB to generate blue signal. Meanwhile, the TMB oxidation product (oxTMB) effectively quenched the fluorescence of GSH-AuNCs to generate fluorescence signal. By modifying antibody onto this probe, linking them to the target OTA, an ultra-sensitive dual-mode OTA immunoassay was achieved. This immunoassay demonstrated excellent sensitivity. The limit of detection (LOD) of colorimetric mode was 0.09 ng/mL, and the LOD of fluorescence mode was 0.02 ng/mL. Besides, the immunoassay had high specificity toward OTA and satisfactory recovery rate in corn samples. Download: Download high-res image (135KB) Download: Download full-size image
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