JOURNAL OF PORPHYRINS AND PHTHALOCYANINES
·
2019
·Vol. 23
·期 09
·1013-1019
·
IF 1.292
Shen Shen,Zihui Zhai,Jiaqi Qin,Xue Zhang,Yujiang Song
The employment of inexpensive metallomacrocycles to create non-precious metal electrocatalysts (NPMEs) with high performance remains a challenge. Herein, we report the self-assembly of low-cost and abundant hemin on carbon black (EC600) under hydrothermal conditions in combination with subsequent pyrolysis, leading to a new NPME. Our NPME exhibits a half-wave potential of 0.89 V vs . reversible hydrogen electrode (RHE), an onset potential of 1.0 V vs . RHE and an average HO 2 − yield below 2% as well as high durability toward oxygen reduction reactions (ORR) in alkaline electrolytes, ranking at the top of all reported NPMEs derived from hemin.
研究领域:
材料科学
·
电化学
·
催化科学
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DOI
高影响因子
MICROCHIMICA ACTA
·
2026
·Vol. 193
·期 8
·518
·
IF 5.4
Gu Tiance,Shi Haiyan,Liu Dongying,Wang Menghan,Wu Qingqing,Du Shuting,Shi Xueran,Wang Jing,Han Qian
A cerium ion-modified hemin polymer (Ce-hemin) was synthesized via a facile route, exhibiting photo-responsive oxidase-like activity and excellent stability. The Ce doping effectively prevented hemin aggregation, facilitated electron transfer, enhanced photocatalytic activity, and created a high adsorption capacity for tetracyclines (TCs). Taking advantage of the photo-sensitivity of TCs, a “turn-on” colorimetric sensing platform based on Ce-hemin nanozymes and smartphone-readout was constructed for visual analysis of TCs. This sensor offers a wide detection range and a low limit of detection (0.11 μM). The mechanism of the “turn-on” colorimetric performance was systematically discussed, which involves the abundant generation of superoxide radical anion (·O 2 − ) species through photoexcitation of the surface complex between TCs and Ce-hemin. Furthermore, machine learning was employed for the accurate classification and identification of two representative TCs and chloramphenicol. Thus, this work provides an environmentally friendly strategy for the detection of TCs without H 2 O 2 , and also broadens the application of photo-responsive nanozymes in colorimetric sensors. Graphical
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高影响因子
MICROCHEMICAL JOURNAL
·
2026
·
IF 5.2
Dongming Xu,Ziyang Xu,Hailan Gao,Xiaoyu Peng,Nan Luo,Yinuo Wang,Minghua Zhang,Shu-xuan Liang,Yongqiang Cheng,Jiangyan Zhang
Hemin-loaded amphiphilic nanoparticles enable stabilized luminol chemiluminescence. • Glow-type chemiluminescence allows direct smartphone imaging without excitation. • AChE inhibition is coupled with chemiluminescent readout for OP quantification. • Reliable OP analysis is achieved in food and environmental samples. Hemin-loaded amphiphilic nanoparticles (HeNPs) were engineered to stabilize hemin and enhance its catalytic performance in the luminol–H₂O₂ chemiluminescence (CL) system. The confined hydrophobic microenvironment effectively suppressed hemin aggregation and produced a stable glow-type emission, which could be directly captured by a smartphone without external excitation or optical accessories. By integrating this stabilized chemiluminescent platform with an acetylcholinesterase (AChE) inhibition mechanism, organophosphorus pesticides (OPs) were translated into quantifiable CL responses. The smartphone-based readout enabled sensitive and reliable analysis with good linearity. The proposed method was further validated in lake water and Chinese cabbage samples, where the results obtained by smartphone imaging showed good agreement with those from microplate-based CL detection and GC–MS. Overall, this work demonstrates a smartphone-enabled chemiluminescent sensing strategy for monitoring OP residues in food and environmental samples. Download: Download high-res image (118KB) Download: Download full-size image
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CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
·
2020
·Vol. 36
·期 2
·301-306
·
IF 1.063
Liu Shuo,Yang Mingjie,Guo Weiwei
Metal-organic frameworks(MOFs)-based nanozyme plays an important role in biosensing, therapy and catalysis. In this study, the effects of single-stranded DNA(ssDNA) with programmable sequences and its complementary DNA(TDNA) on the intrinsic peroxidase-like activity of hemin loaded MOFs(UiO-66- 2 ), denoted as hemin@UiO-66-NH 2 , were investigated. The hemin@UiO-66- 2 exhibited improved catalytic activity compared with free hemin. However, the catalytic activity is inhibited in the presence of ssDNA, as ssDNA can be adsorbed by MOFs and therefore protected the active sites from contact with substrates. Upon the addition of the T DNA , double-stranded DNA(dsDNA) was formed and detached from the MOFs, resulting in the recovery of catalytic activity. Sequentially adding ssDNA or its complementary DNA strands can achieve the reversible regulation of the catalytic activity of MOFs nanozymes. Moreover, the DNA hybridization-based regulation was further applied to a cascaded catalytic system composed of the nanozyme, hemin@UiO-66-NH 2 , and glucose oxidase. These nanozyme based programmable and reversibly regulated catalytic systems may have potential applications in future smart biosensing and catalysis systems.
研究领域:
生物化学
·
纳米医学
·
催化科学
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DOI
高影响因子
Materials Science & Engineering C-Materials for Biological Applications
·
2020
·
IF 5.88
Chunhua Lin,Yue Du,Shiqi Wang,Li Wang,Yonghai Song
The work presents a novel glucose oxidase@Cu-hemin metal-organic frameworks (GOD@ Cu-hemin MOFs) with a ball-flower structure as bienzymatic catalysts for detection of glucose. The GOD@Cu-hemin MOFs exhibits great stability as compared with free horseradish peroxidase and GOD toward harsh conditions because the ball-flower-like shell of Cu-hemin MOF effectively protects from GOD. Thus, the GOD@Cu-Hemin MOFs can be used in external harsh conditions such as high temperature and acid/base. The GOD@Cu-hemin MOFs is capable of sensitive and selective detection of glucose via peroxidase-like of Cu-hemin MOFs and GOD by using 3,3′,5,5′-tetramethylbenzidine (TMB) as a substrate. Under the existence of glucose, O 2 is reduced into H 2 O 2 via GOD@Cu-hemin MOFs. The produced H 2 O 2 as well as Cu-hemin MOFs oxidize TMB into blue oxTMB which shows UV–Vis absorbance at 652. The absorption intensity of oxTMB linearly increases with the increasing concentration of glucose from 0.01 to 1.0 mM with detection limit of 2.8 μM. An integrated agarose hydrogel film (Aga/GOD@Cu-hemin MOF/TMB) sensor is rationally designed for colorimetric detection of glucose. The sensor displays a response range of 30 μM-0.8 mM with a detection limit of 0.01 mM. The result indicates that the Cu-hemin MOFs are an ideal carrier for the encapsulation of enzymes.
研究领域:
生物化学
·
材料科学
·
分析化学
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DOI
高影响因子
JOURNAL OF ENVIRONMENTAL ENGINEERING
·
2022
·Vol. 148
·期 8
·04022036
·
IF 2.479
Jia Deng,Xiaobao Zeng,Feng Wu,Zheng Fang,Yitao Dai,Li-Zhi Huang
Metal porphyrin structure is believed to be the active site for microbiological reductive dechlorination. In this work, a biomimetic Hemin catalyst was used for the electrochemical dechlorination of trichloroethylene (TCE). Cyclic voltammetric analysis demonstrates the high electrochemical reactivity of Hemin toward reductive dechlorination of TCE, which is further confirmed by controlled potential electrolysis. TCE can be fully dechlorinated to acetylene, ethylene, and ethane. Density functional theory calculations show the appearance of unpaired electron spins on coordinatively unsaturated Fe sites. The breakage of the Fe–Cl bond in Hemin with the formation of coordinatively unsaturated FeII sites shows reactivity toward TCE-to-dichloroethylene (DCE) conversion, but FeII sites with the coordination to Cl atoms have no reactivity. Lowering the applied potential to −2.1 V generates coordinatively unsaturated FeI sites, showing much higher reactivity toward TCE reduction compared with FeII sites. The energy barrier involving complete dechlorination of TCE was decreased from 1.83 eV by FeII sites to 0.86 eV by FeI sites. FeI sites can continue to contribute to TCE dechlorination in electrochemical systems. Our study provides a novel biomimetic electrochemical approach for the dechlorination of TCE in groundwater.
研究领域:
环境化学
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电化学
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催化
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材料科学
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理论化学
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DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2016
·
IF 4.758
Gonge Tang,Cailan Zhao,Jie Gao,Hongliang Tan
It is of great importance to simply and sensitively detect hydrogen sulfide (H 2 S) because of its role in various physiological processes as well as its inherent toxicity. In this work, a colorimetric method for H 2 S detection was developed by employing terbium-G-quadruplex-hemin (Tb/G4-hemin) DNAzyme as a peroxidase mimic, which can catalyze H 2 O 2 -mediated oxidation of 2,2′-azinobis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) to produce radical cation (ABTS + ). Compared with the G4-hemin DNAzyme promoted by K + and Na + , Tb/G4-hemin DNAzyme exhibits a higher catalytic activity. In the presence of Ag + , the peroxidase-like activity of Tb/G4-hemin DNAzyme can be inhibited significantly owing to the disruption of G-quadruplex structure. However, the addition of H 2 S can effectively suppress such negative behavior by competitive binding with Ag + , leading to the recovery of the peroxidase-like activity of Tb/G4-hemin DNAzyme, which can be reflected by an increase in absorbance signal of ABTS + . The absorbance of ABTS + was enhanced linearly with increasing H 2 S concentration from 20 nM to 2 μM. The detection limit for H 2 S is 13 nM, which is much lower than most of previous methods. Moreover, the proposed method possesses the features of simple preparation, easy reproducibility and good biocompatibility. Given the fine performances and striking properties, we believe that the Tb/G4-hemin DNAzyme would have a great promise for analytical applications.
研究领域:
分析化学
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生物化学
·
分子生物学
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高影响因子
Inorganics
·
2023
·Vol. 11
·期 6
·226
·
IF 2.9
Ya-Ting Gao,Shuai Chang,Bin-Bin Chen,Da-Wei Li
Constructing optical nanoprobes with superior performance is highly desirable for sensitive and accurate assays. Herein, we develop a facile room-temperature strategy for the fabrication of green emissive carbon nanoclusters (CNCs) with dual-exciting centers for the dual-channel sensing of hemin. The formation of the CNCs is attributed to the crosslinking polymerization of the precursors driven by the Schiff base reaction between ethylenediamine and 2,3-dichloro-5,6-dicyano-1,4-benzoquinone. Most importantly, the proposed CNCs have a unique excitation-independent green emission (518 nm) with two excitation centers at 260 nm (channel 1) and 410 nm (channel 2). The dual-exciting central emission can serve as dual-channel fluorescence (FL) signals for highly sensitive and reliable detection of hemin based on the inner filter effect. Because of the great spectral overlap difference between the absorption spectrum of hemin and the excitation lights of the CNCs in the two channels, hemin has a different quenching effect on FL emission from different channels. The dual-channel signals of the CNCs can detect hemin in the range of 0.075–10 μM (channel 1) and 0.25–10 μM (channel 2), respectively. These findings not only offer new guidance for the facile synthesis of dual-exciting central CNCs but also establish a reliable sensing platform for the analysis of hemin in complex matrixes.
研究领域:
纳米材料科学
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分析化学
·
生物医学工程
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产品关联
DOI
高影响因子
Materials Chemistry Frontiers
·
2021
·Vol. 5
·期 16
·6041-6051
·
IF 6.482
Arbab Ali,Jiaming Liu,Huige Zhou,Tao Liu,Muhammad Ovais,Huibiao Liu,Yukui Rui,Chunying Chen
Surgical site infections are the most common and serious concern in health care settings. Currently, systemic administration of prophylactic antibiotics or local disinfection treatment is prescribed after surgical procedures. However, prolonged use of antibiotics may pose adverse side effects, bacterial resistance and hospitalization costs. Recently, the use of nanomaterials with peroxidase enzyme-mimic activity has drawn higher consideration due to their high antibacterial potential and minimum biotoxicity. Hemin is a highly active molecule and also the active source of many biological enzymes, such as peroxidase, which could efficiently catalyze various biochemical reactions. However, the insolubility of hemin makes it easy for it to aggregate and lose its catalytic ability. Here, we designed a well-dispersed hemin/graphdiyne (GDY) nanocomposite (GDY–hemin) with high peroxidase activity. GDY–hemin can decompose hydrogen peroxide (H2O2) to generate highly-toxic hydroxyl radicals that impair bacterial cell membranes, ultimately leading to bacterial death in both in vitro and in vivo experiments. The results reveal that GDY–hemin has good biocompatibility and is suitable for wound infection treatment in vivo.
研究领域:
纳米技术
·
生物医学工程
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材料科学
·
伤口护理
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高影响因子
Scientific Reports
·
2016
·Vol. 6
·期 1
·1-9
·
IF 5.228
He Juan,Yang Han,Zhang Yayun,Yu Jie,Miao Longfei,Song Yonghai,Wang Li
Herein, a smart porous material, Cu-hemin metal-organic-frameworks (Cu-hemin MOFs), was synthesized via assembling of Cu 2+ with hemin to load glucose oxidase (GOD) for electrochemical glucose biosensing for the first time. The formation of the Cu-hemin MOFs was verified by scanning electron microscopy, X-ray powder diffraction, Fourier transform infrared spectroscopy, N 2 adsorption/desorption isotherms, UV-vis absorption spectroscopy, fluorescence spectroscopy, thermal analysis and electrochemical techniques. The results indicated that the Cu-hemin MOFs showed a ball-flower-like hollow cage structure with a large specific surface area and a large number of mesopores. A large number of GOD molecules could be successfully loaded in the pores of Cu-hemin MOFs to keep their bioactivity just like in a solution. The GOD/Cu-hemin MOFs exhibited both good performance toward oxygen reduction reaction via Cu-hemin MOFs and catalytic oxidation of glucose via GOD, superior to other GOD/MOFs and GOD/nanomaterials. Accordingly, the performance of GOD/Cu-hemin MOFs-based electrochemical glucose sensor was enhanced greatly, showing a wide linear range from 9.10 μM to 36.0 mM and a low detection limit of 2.73 μM. Moreover, the sensor showed satisfactory results in detection of glucose in human serum. This work provides a practical design of new electrochemical sensing platform based on MOFs and biomolecules.
研究领域:
生物传感
·
材料科学
·
电化学
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2024
·
IF 6.7
Bin Liu,Tian Wang,Dehui Qiu,Xinrong Yan,Yuan Liu,Jean-Louis Mergny,Xiaobo Zhang,David Monchaud,Huangxian Ju,Jun Zhou
Hemin/G-quadruplex (hG4) complexes are frequently used as artificial peroxidase-like enzymatic systems (termed G4 DNAzymes) in many biosensing applications, in spite of a rather low efficiency, notably in terms of detection limits. To tackle this issue, we report herein a strategy in which hemin is chemically modified with the amino acids found in the active site of parent horseradish peroxidase (HRP), with the aim of recreating an environment conducive to high catalytic activity. When hemin is conjugated with a single arginine, it associates with G4 to create an arginine–hemin/G4 (R-hG4) DNAzyme that exhibits improved catalytic performances, characterized by kinetic analysis and DFT calculations. The practical relevance of this system was demonstrated with the implementation of biosensing assays enabling the chemiluminescent detection of G4-containing DNA and colorimetry detection of the flap endonuclease 1 (FEN1) enzyme with a high efficiency and sensitivity. Our results thus provide a guide for future enzyme engineering campaigns to create ever more efficient peroxidase-mimicking DNA-based systems.
研究领域:
生物化学
·
分子生物学
·
化学生物学
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Pengjuan Ni,Chuanxia Chen,Yuanyuan Jiang,Yizhong Lu,Wei Chen
Hemin, the most common biological iron form, serves many biological functions. However, studies on hemin detection are scarce. As an unusual natural peroxide, artemisinin is quite stable in the presence of common species. While, it can decompose to generate superoxide radicals in the presence of hemin. And the as-obtained superoxide radicals can effectively oxidize thiamine to form fluorescent thiochrome. Inspired by this, a simple and novel fluorescent assay for hemin detection based on artemisin-thiamine is developed. Based on the linear relationship between the fluorescence intensity and concentration of hemin, the developed artemisinin-thiamine assay can be utilized for sensitive detection of hemin in the concentration range of 2.0–300.0 nM with a detection limit of 0.68 nM. Moreover, this assay shows high selectivity over other cations and biomolecules. Besides, hemin can be detected in serum samples with satisfactory results, which demonstrates its great potential for real sample analysis. The designed assay is free of any fluorescent nanomaterials or probes by just mixing these reagents together, and all the detection processes is performed within 20 min. Therefore, it can offer a simple, highly sensitive and selective sensing platform for fluorescence detection of hemin.
研究领域:
生物化学
·
分析化学
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DOI
高影响因子
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