TALANTA
·
2018
·
IF 4.244
Yuqing Zhang,Gongwei Sun,Yi Zhang,Biao Huang,Zhi Xing,Sichun Zhang,Xinrong Zhang
Inductively Coupled Plasma Mass Spectrometry (ICP-MS) based immunoassay method has been proposed in multiple immunoassays but has not been used in competitive and sandwich formats immunoassay simultaneously. The two immunoassays were usually conducted separately in clinical field depending on the size and the amount of binding sites of targets. We proposed an immunoassay method based on magnetic beads and ICP-MS detection that could be suitable for both small and large molecules. The functionalized magnetic beads were added to capture the immune complex after immune reaction. In this way, thyrotropin and free thyroid hormone can be captured, separated and then detected according to the elemental tags by ICP-MS simultaneously. The new method was evaluated by comparing the results with time resolved fluorescence immunoassays (TRFIA). The dynamic ranges of TSH and FT 4 were 0.16–105 mU/L and 3.5–65 pmol/L, respectively. The limits of detections were 0.06 mU/L for TSH and 1.59 pmol/L for FT 4 . And the relative standard deviations (RSD) of TSH and FT 4 were 4.64% at 2.5 mU/L and 1.87% at 5.85 pmol/L. This immunoassay method enables the determination of small and large biomolecules simultaneously via competitive and sandwich immunoassay formats.
研究领域:
分析化学
·
临床化学
·
生物医学工程
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DOI
PubMed
高影响因子
GEOSTANDARDS AND GEOANALYTICAL RESEARCH
·
2024
·
IF 2.7
Qiuyun Guan,Xudong Guo,Yali Sun,Xiaoming Liu,Shouqian Zhao
A simple method was developed for the determination of REEs, Zr, Hf, Th and U in ultramafic rocks by inductively coupled plasma-mass spectrometry with a combination of RE and UTEVA extraction resins for their separation and pre-concentration. Ultramafic rocks were digested with HNO 3 -HF-HClO 4 and finally turned into 11 mol l −1 HCl solutions together with H 3 BO 3 to remove insoluble fluorides. The removal of matrix elements was achieved during the loading procedure. Following this, REEs on RE resin, and Zr, Hf, Th and U on UTEVA resin were eluted with 10 ml of 0.24 mol l −1 HCl, with recoveries better than 94.4%. This method was validated using reference materials JP-1, DTS-2B, OKUM, UB-N, MUH-1 and DZΣ-2, and the measurement results for target analytes were comparable to literature values, indicating its applicability to the determination of REEs, Zr, Hf, Th and U at ultra-trace level in ultramafic rocks.
研究领域:
地球化学
·
分析化学
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产品关联
DOI
高影响因子
TALANTA
·
2017
·
IF 4.162
Yupin Cao,Biyang Deng,Lizhen Yan,Hongli Huang
An environmentally friendly and highly efficient gas pressure-assisted sample introduction system (GPASIS) was developed for inductively-coupled plasma mass spectrometry. A GPASIS consisting of a gas-pressure control device, a customized nebulizer, and a custom-made spray chamber was fabricated. The advantages of this GPASIS derive from its high nebulization efficiencies, small sample volume requirements, low memory effects , good precision, and zero waste emission. A GPASIS can continuously, and stably, nebulize 10% NaCl solution for more than an hour without clogging. Sensitivity, detection limits, precision, long-term stability, double charge and oxide ion levels, nebulization efficiencies, and matrix effects of the sample introduction system were evaluated. Experimental results indicated that the performance of this GPASIS, was equivalent to, or better than, those obtained by conventional sample introduction systems. This GPASIS was successfully used to determine Cd and Pb by ICP-MS in human plasma.
研究领域:
分析化学
·
环境科学
·
医学
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DOI
PubMed
高影响因子
ELECTROPHORESIS
·
2020
·Vol. 42
·期 4
·465-472
·
IF 3.081
Qiuyue Xu,Beibei Chen,Man He,Bin Hu
Ti (IV)-modified vinyl phosphate magnetic nanoparticles (Fe 3 O 4 @SiO 2 @KH570-PO 4 -Ti (IV)) was prepared for simultaneous extraction of multiple arsenic species, followed by high performance liquid chromatography (HPLC)– inductively coupled plasma mass spectrometry (ICP-MS) analysis. Inorganic arsenic (iAs), dimethyl arsenic acid (DMA), monomethyl arsenic acid (MMA), p -amino phenyl arsenic acid ( p -ASA), 4-hdroxyphenylarsenic acid (4-OH), phenyl arsenic acid (PAA), and 3-nitro-4-hydroxyphenylarsenic acid (ROX) were investigated as interest analytes. It was found that they were quantitatively adsorbed on Fe 3 O 4 @SiO 2 @KH570-PO 4 -Ti (IV) at pH 5, and desorbed completely with 0.1 mol/L sodium hydroxide solution. Enrichment factor of 100-fold was obtained by consuming 100 mL sample solution. Under the optimal conditions, the method combining MSPE with HPLC-ICP-MS presented a linear range of 1–5000 ng/L for seven arsenic species. The limits of detection were 0.39, 0.60, 0.23, 1.85, 0.54, 0.48, and 0.84 ng/L for DMA, MMA, p -ASA, iAs, 4-OH, PAA, ROX, with the relative standard deviations ( c = 10 ng/L, n = 7) of 3.6, 3.9, 5.5, 12.4, 6.1, 5.8, 5.0, respectively. The accuracy of the method was validated by analyzing BCR 627 Tuna fish. The application potential of the method was further evaluated by chicken muscle and liver samples. No target arsenic species were detected in these samples, and good recoveries (80.6–123%) were obtained for the spiked samples at low, medium, and high concentration levels.
研究领域:
分析化学
·
环境科学
·
食品科学
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DOI
高影响因子
SPECTROCHIMICA ACTA PART B-ATOMIC SPECTROSCOPY
·
2024
·
IF 3.2
Yinzhong Gao,Heping Jiao,Zhenhua Wang,Jingbo Chao,Jian Li,Ligang Hu,Shengxiao Zhang,Jing Sun
A core-shell magnetic covalent organic frameworks (MCOF-SN) nanocomposite with high adsorption capacity was prepared by simply and directly coating covalent organic frameworks with abundant thione and secondary amine groups on the surface of magnetic nanoparticles, which were used to selectively extract copper (Cu 2+ ), cadmium (Cd 2+ ), lead (Pb 2+ ), cobalt (Co 2+ ) and nickel (Ni 2+ ) ions prior to the detection by ICP-MS. The prepared magnetic covalent organic frameworks have high adsorption capacity of 52, 65, 162, 34 and 42 mg g −1 for Cu 2+ , Cd 2+ , Pb 2+ , Co 2+ and Ni 2+ ions, respectively. The analytical parameters affecting magnetic solid-phase extraction (MSPE) of metal ions were optimized. They were pH of sample, 4–5; eluent, 1 mL of 1 % ( v /v) HNO 3 ; amount of adsorbent, 15 mg; and sample volume, 200 mL. Under optimal experimental conditions, the limits of detection (LODs) of Cu 2+ , Cd 2+ , Pb 2+ , Co 2+ and Ni 2+ ions for water are 0.26, 0.16, 0.25, 0.08 and 0.17 ng L −1 with enrichment factors of 192, 191, 196, 188 and 190, and their relative standard deviations (RSD) were less than 5 % (20 ng L −1 , n = 3), respectively. The LODs for Chinese herbal medicine samples were 0.52, 0.32, 0.50, 0.16 and 0.34 μg kg −1 based on 0.100 g sample, respectively. The proposed MSPE-inductively coupled plasma mass spectrometry (MSPE-ICP-MS) method with simple operation, high sensitivity and high anti-matrix interference ability has been successfully applied to the samples with complicated matrices, such as water and Chinese herbal medicine.
研究领域:
分析化学
·
环境科学
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产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2020
·
IF 3.594
Yu Hua,Jia-yuan Li,Hong Min,Xiao-hong Wu,Xiao-bing Cui,Yi-jun Chen,Hong-zhen Lian,Dong Sheng
In this work, by introducing solvothermal method for generating Fe 3 O 4 nanoparticles and “one-pot” method for synthesizing organic–inorganic hybrid monolithic vinyl functionalized Fe 3 O 4 (Fe 3 O 4 @HM), a facile procedure was proposed for preparing poly(1-vinylimidazole) (PVIM) functionalized gold ion imprinted polymer based MNPs@IIP (MNPs@HM@PVIM-Au-IIP). The as-prepared magnetic nanocomposites were characterized by scanning electron microscopy (SEM), transmission electron microscope (TEM), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), thermogravimetric analysis (TGA), and vibrating sample magnetometry (VSM), etc. After the optimum conditions, including solution pH, adsorption time and sample volume, eluent composition and volume, and elution time were investigated, this MNPs@IIP was employed as ion imprinted polymer magnetic solid phase extraction (II-MSPE) matrix for separation and enrichment of trace gold ions followed by inductively coupled plasma mass spectrometric (ICP-MS) determination with excellent selectivity and sensitivity, and strong anti-interference ability. These merits were benefited from the formation of hybrid monolithic interlayer enduing the resultant MNPs@HM@PVIM-Au-IIP with good chemical and mechanical stability, as well as the dimensional homogeneity due to the solvothermal-generated Fe 3 O 4 nanoparticles as the cores. The applicability superiority of this MNPs@IIP was confirmed by the rapid and efficient extraction of trace level Au(III) in environment water, sediment, soil, coal and ore samples with satisfactory results. The reliability of this II-MSPE-ICP-MS method was also verified by using corresponding Certified Standard Reference materials and spiking recovery of the real samples analyzed.
研究领域:
分析化学
·
环境科学
·
材料科学
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产品关联
DOI
高影响因子
TALANTA
·
2023
·
IF 6.1
Xiao Tian,Lei Zhao
Concentrations of trace elements in coal and coal combustion products are commonly analyzed by inductively coupled plasma-mass spectrometry (ICP-MS) due to large number of elements detected and the relatively the low detection limits of this technique. Like other geological samples, complete dissolution of coal and coal combustion products is also essential for accurate ICP-MS results. In this study, Sr and Ba in coal and coal combustion products (fly and bottom ashes) from two coal-combustion power plants from North China analyzed by XRF and ICP-MS were comparatively studied. The concentrations of Sr and Ba analyzed by ICP-MS, when a mixture of acids (2 ml HF + 5 ml HNO 3 for each 50 mg coal sample and 5 ml HF + 2 ml HNO 3 for each 50 mg ash sample) was used for microwave-assisted digestion, do not fit well with the their relative XRF results. This is most probably due to the formation of the fluorides during microwave digestion, and this assumption is supported by the presence of various fluoride compounds, NaMgAl(F, OH) 6 ·H 2 O, NH 4 MgAlF 6 , AlF 3 , and K 2 SiF 6 , in the residues of all the coal and ash samples in our sequential extraction experiment. Cations of Sr and Ba were probably trapped into the divalent cation sites of the fluorides. Concentrations of Sr and Ba analyzed by ICP-MS using increased HF and HF/HNO 3 ratio (7 ml HF and 2 ml HNO 3 for each 50 mg coal/ash sample) are in better agreement with the XRF results. Our results indicate that excess amount of HF probably has led to the suppression of these elements due to fluoride precipitation. The results indicate that the modified digestion method is capable of achieving complete digestion of coal samples and results in a reliable analysis of Sr and Ba concentrations in coal samples and most fly ashes by ICP-MS. However, the formation of insoluble fluorides is probably not completely suppressed for some bottom ash samples, which can result in underestimation of Sr and Ba concentrations. Nevertheless, XRF analysis can serve as a reliable cross-check method to assist in the evaluation of the accuracy of ICP-MS results.
研究领域:
环境科学
·
分析化学
·
地球化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY
·
2022
·Vol. 38
·期 1
·142-155
·
IF 4.351
Jia-Xin She,Weiqiang Li,Shichao An,Yuanfeng Cai
Stable Sn isotopes have the potential to constrain the behavior of Sn in various natural and anthropogenetic processes, however, studies on stable isotopes of Sn for terrestrial rock samples have been limited. Here, we present a high-precision Sn isotope analytical method of geological samples by the double spike technique. Samples were equilibrated with a 117Sn–122Sn double spike during dissolution, then Sn was purified from the rock matrix using TRU spec resin. The purified Sn solutions were analyzed on a Nu 1700 Sapphire MC-ICP-MS, and data reduction was performed using Newton–Raphson iteration. Effects of acid mismatch, concentration mismatch, and matrix elements were systematically tested to evaluate their impact on the accuracy and repeatability of the measurements. We show that the mismatch of acidity exerts negligible influences on the Sn isotope analysis. By contrast, the accuracy of Sn isotopic measurements could be compromised if the Sn concentration of the sample is below 40% of that of the standard. To ensure the accuracy of Sn isotope analysis, the concentration match between the sample and standard should be better than ±20%. When the measured solution has varying mass ratios of [X]/Sn (ng g−1/ng g−1) lower than 0.5 for Mg, Ca, Fe, Ti, U, Ni, Ag, As, Ru, and Mo, the measured Sn isotope composition was not affected. However, Cd in Sn samples could affect the accuracy of Sn isotope analysis by the 117Sn–122Sn double spike through the generation of 116CdH+ in plasma that interferes with 117Sn+. The Cd/Sn ratio needs to be below 0.01 to ensure analytical accuracy by the double spike method. The accuracy of this method was further verified by measuring the pure solution with both double spike and sample standard bracketing methods, as well as processing a synthetic solution through column chemistry. The Sn isotope compositions of geological samples measured using our method are reproducible and consistent with previous data from other laboratories. Additionally, Sn isotope data for geological reference materials BIR-1, RGM-1, and GSR-1 are reported for the first time. Based on repeated analyses of pure and geological reference materials, the long-term intermediate precision is better than ±0.069‰ on δ122/118Sn for a spiked solution of 100 μg g−1.
研究领域:
地球化学
·
分析化学
·
地质学
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DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Yujing He,Jing Hu,Siqi Wei,Chenghui Li,Xiandeng Hou
As an essential and universal enzyme, alkaline phosphatase (ALP) has been identified as an important biomarker of various diseases, thus highly sensitive assay of ALP activity is of great significance. Herein, a label-free and sensitive ICP-MS based assay was developed for ALP activity detection using MnO 2 nanosheets (MnO 2 NS) as the element target source. ALP triggered the hydrolysis of ascorbic acid-2-phosphate (AAP) to produce reductive ascorbic acid (AA), followed with the decomposition of MnO 2 NS to Mn 2+ ions, which were quantified directly by ICP-MS after selective separation of MnO 2 NS and Mn 2+ ions simply by filtration, and no further elemental labelling was needed. Under the selected conditions, the limit of detection (LOD) was 0.007 U L -1 for ALP. Additionally, this assay was also extended to investigate the inhibition effect of ALP inhibitor (Na 3 VO 4 ) with reasonable results. To our knowledge, this is the first endeavor for ALP activity detection using ICP-MS, which holds great promise for the early clinical diagnosis and biomedical research on ALP.
研究领域:
分析化学
·
生物化学
·
纳米技术
·
医学诊断
✓ 已验证
产品关联
DOI
高影响因子
GEOSTANDARDS AND GEOANALYTICAL RESEARCH
·
2025
·
IF 3.4
Ai-qun Xiao,Pei-pei Zhao,Lu Yin,Bei Xu
A single-column extraction chromatographic purification method for tungsten using the N-benzoyl-N-phenylhydroxylamine (BPHA) resin was investigated in this study. This method greatly simplifies the W purification procedure, and only 10 ml of acid (5 ml 3 mol l -1 HCl-0.1 mol l -1 HF and 5 ml 1 mol l -1 HF) is required to separate W from Ti, Zr, Hf, Ta and other matrix elements. The blank in the separation step was less than 0.1 ng, and the recovery of W was ~ 99%. Six independent digestion measurements of reference materials BHVO-2, BCR-2, RGM-2 and JB-2 yielded μ 182 W values (mean value with intermediate precision, 95% confidence level) of -10.3 ± 4.0 ( n = 6, 2 s )，-1.2 ± 5.3 ( n = 6, 2 s )，-1.4 ± 4.4 ( n = 6, 2 s ) and 0.6 ± 3.8 ( n = 6, 2 s ), respectively, which are in good agreement with the previously reported values.
研究领域:
地球化学
·
分析化学
·
同位素地质学
·
质谱分析
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产品关联
DOI
高影响因子
Analytical Methods
·
2017
·Vol. 9
·期 17
·2546-2552
·
IF 1.9
Weizhe Hong,Gongwei Sun,Yi Zhang,Zhi Xing,Biao Huang,Sichun Zhang,Xinrong Zhang
ICP-MS-based multiplex immunoassays have the advantages of low sample consumption and minimized repetitions of tedious procedures. However, this technique is seldom used in clinical tests because of the complicated matrix effect in clinical samples and the discrimination effect to the target proteins. In this work, we developed an ICP-MS-based magnetic immunoassay by using magnetic beads as highly efficient affinity probes and optimizing the magnetic immunoassay parameters. We applied this method to the simultaneous determination of three crucial gynecological tumor biomarkers that are vital in the prognosis and monitoring of the recurrence of breast cancer and ovarian cancer. The measurable ranges of HER-2, HE4 and CA15-3 were 12–1000 ng mL−1, 8–2000 pmol L−1 and 5–200 U mL−1, with detection limits (3σ) of 3.94 ng mL−1, 2.59 pmol L−1 and 1.62 U mL−1, respectively. The relative standard deviations (RSDs) were 2.61% for HER-2 at 15 ng mL−1, 4.90% for HE4 at 25 pmol L−1 and 6.68% for CA15-3 at 10 U mL−1, indicating good accuracy of the method. The spiking test indicated that the present method had a low matrix effect. Under optimized conditions, the simultaneous detection of HER-2, HE4, and CA15-3 in clinical serum samples was performed and the values correlated well with those obtained by the time resolved fluorescence immunoassay (r2 = 0.9568 for HER-2, 0.9485 for HE4, 0.9133 for CA15-3). The present work shows that the ICP-MS-based magnetic immunoassay meets the requirements for the quantification of three crucial gynecological tumor biomarkers in real clinical tests.
研究领域:
分析化学
·
医学诊断
·
癌症研究
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产品关联
DOI
高影响因子
Food Analytical Methods
·
2016
·Vol. 9
·期 12
·3501-3508
·
IF 2.167
Shao Yufang,Chen Ying,Liu Jian,Wu Pingping,Ying Yibin,Xie Jing
High-resolution inductively coupled plasma mass spectrometry (ICP-MS) was applied to determine potential toxic element pollution in rice and paddy soil in a lead-acid battery production area. The contamination levels of chromium (Cr), cadmium (Cd), and lead (Pb) in rice and soil at three sampling areas were determined, and the uptake mechanism of Cr, Cd, and Pb from soil to rice grain was preliminarily analyzed. The results show that the range of potential toxic elements in rice concentrations is 3.914–11.788, 0.057–1.215, and 0.187–1.585 mg kg −1 for Cr, Cd, and Pb, respectively. For the paddy soils, Cd concentration is 0.610 mg kg −1 , which exceeds 0.300 mg kg −1 , a value recommended by Chinese Environmental Quality. Bioaccumulation factor (BAF, an index of the ability of plant to absorb a particular metal with respect to its concentration in the soil substrate) values of Cr, Cd, and Pb were less than 1 in all rice samples, suggesting that the bioavailability of these metals was relatively low in rice. The correlations between BAF values with organic matter (OM) and pH were calculated to find the mobility and availability of potential toxic elements in soil. At last, the estimated daily intake (EDI) dose was determined to evaluate the health risk to local population via consumption of rice contaminated with Cr, Cd, and Pb. This study indicated that the accumulation and translocation of potential toxic elements from soil to rice could cause the health risk for the local population.
研究领域:
环境科学
·
农业化学
·
毒理学
·
公共卫生
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）