ADVANCED MATERIALS
·
2025
·
IF 26.8
Guoqian Li,Langlang Yi,Shiya Zhang,Qin Song,Minghui Liang,Zhihui Guan,Yao Zhang,Yuan Zhuang,Bo Hou,Xinyuan Wang,Xiaoyao Wu,Pengju Yin,Klyuyev Dmitriy,Jiang Yang,Bo Hu,Jie Tian
Surface-enhanced Raman scattering (SERS) technology has been extensively employed for the detection of liquid samples in the biomedical field due to high sensitivity and non-invasive characteristics. However, quantitative SERS detection is hindered by sophisticated instrumentation and complex calibration procedures, while qualitative analyses often provide insufficient concentration information for clinical diagnosis. Herein, a concentration indicator kit based on dandelion propagation-inspired SERS strategy is developed for the semi-quantitative detection of drug residues in urine. This strategy achieves significant signal enhancement through the synergistic effects of optimized hotspot construction and molecular adsorption, resulting in an analytical enhancement factor of 2.503 × 10⁵, which is 4143 times greater than that of silver nanoparticles. An innovative phenomenon termed the “concentration indicator” is discovered, wherein the SERS signal is dramatically enhanced at specific indicated concentrations while remaining weak at adjacent concentrations. The practical utility of this kit is validated through the detection of 2-mercaptobenzothiazole in urine samples. The kit exhibits excellent performance in semi-quantitative detection of standard solutions and simulated samples at high, indicated, and low concentrations. The concentration indicator opens new avenues for the development of simplified and reliable SERS-based detection, offering a promising platform for bedside testing in medical diagnostics.
研究领域:
生物医学检测
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纳米技术
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分析化学
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医学诊断
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产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
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2020
·
IF 10.257
Rui Jin,Lianjing Zhao,Xu Yan,Xiaosong Han,Mengqi Liu,Yue Chen,Qingyun Li,Dandan Su,Fangmeng Liu,Peng Sun,Xiaomin Liu,Chenguang Wang,Geyu Lu
Oxalate is commonly employed as adjuvant of pesticide agent, causing renal injury of human even in trace residues. Despite the great achievements of the existing point-of-care testing (POCT) technology, accurate on-site screening of oxalate remains a tricky issue. To this aim, we proposed a “lab in a tube” platform which integrated portable hydrogel kit with smartphone for real-time monitoring of oxalate to achieve quantitatively precise analysis. In this work, a stimuli-responsive hydrogel-based kit was constructed via embedding manganese dioxide (MnO 2 ) nanosheets into sodium alginate hydrogel system. Based on the intrinsic oxidase-like activity, MnO 2 nanosheets-based nanozyme triggered color reaction by introducing a common sensing probe 3,3′,5,5′-tetramethylbenzidine. Meanwhile, the presence of oxalate would decompose MnO 2 nanosheets, inducing the decrease of nanozyme activity, which resulted in the color response of portable kit. Coupling with ImageJ software, the image information of kit captured via smartphone could be transduced into the hue intensity, which provided a directly quantitative tool to detect oxalate with a detection limit of 8.0 μmol L −1 . This portable smartphone biosensor was successfully applied for screening urine sample within 10 min for high-throughput analysis (twelve samples) without the need for any advanced analytical instruments. Based on the merits of simple operation, cost-efficiency, and good selectivity, the availability of the miniaturized biosensor platform for POCT will achieve the requirements of routine screening and disease prevention.
研究领域:
分析化学
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
ENERGY & FUELS
·
2020
·
IF 3.421
Peijing Shao,Han Chen,Qin Ying,Shihan Zhang
In order to fulfill the application of carbonic anhydrase (CA) in the carbon dioxide capture process, immobilization technology will be used to enhance the stability of the enzyme. The structure of the support for the enzyme immobilization is important to determine the enzyme activity and stability. In this work, mesoporous molecular sieves with different dimensions and pore sizes were used as supports for the immobilization of CA. Characterization analysis indicated that the carrier material retained its integrity after enzyme immobilization. The half-life (t1/2) of CA/KIT-6, CA/SBA-15, and CA/MCM-41 increased to 3.0, 2.8, and 2.0 times compared to the free CA enzyme. Additionally, the experimental results showed that when using SBA-15 with a two-dimensional structure and a large pore size as the carrier, the CA enzyme loading was high and retained 78% relative activity and 98% initial activity after 6 days at 40 °C. Comprehensively, CA/SBA-15 was more suitable for capturing CO2 in flue gas than CA/MCM-41 and CA/KIT-6.
研究领域:
生物化学
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环境科学
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产品关联
DOI
高影响因子
Molecular Medicine Reports
·
2021
·Vol. 23
·期 5
·1-9
·
IF 2.952
Yang Yao,Ranyuan Luo,Shu Xiong,Chang Zhang,Yukun Zhang
Intestinal inflammation frequently occurs alongside dysmotility, which is characterized by altered myosin light chain phosphorylation levels. Curcumin, an active component from the ginger family, is reported to confer anti‑inflammatory effects. However, the effects of curcumin on both diarrhea and constipation associated inflammation remains to be elucidated. The present study was designed to investigate the effects of curcumin on diarrhea and constipation and to determine the related mechanisms. Sprague‑Dawley rats were used to establish diarrhea and constipation models via intracolonic acetic acid (4%) instillation or cold water gavage for 2 weeks, respectively. Blood samples were collected to measure the serum levels of the cytokines TNF‑α and IL‑1β using ELISA kits. Western blotting was performed to measure NF‑κB, RhoA, Rho‑related kinase 2, phosphorylated MLC20, phosphorylated myosin phosphorylated target subunit 1, 130k Da‑MLC kinase (MLCK), c‑kit tyrosine kinase protein expression, and reverse transcription‑quantitative PCR was conducted to measure MLCK expression levels. The results indicated that curcumin reversed the elevations in the pro‑inflammatory cytokines IL‑1β and TNF‑α by inhibiting the NF‑κB pathway in rats with diarrhea and constipation. The results also indicated that myosin light chain (MLC) phosphorylation in intestinal smooth muscle was positively and negatively associated with the motility of inflammation‑related diarrhea and constipation in rats, respectively. Curcumin significantly reversed the increased MLC phosphorylation in the jejunum of the rats with diarrhea, significantly enhanced the reductions in inflammatory mediators, including TNF‑α and IL‑1β, of rats with constipation and significantly ameliorated the related hyper‑motility and hypo‑motility in rats with both diarrhea and constipation. In conclusion, the potential roles of the MLC kinase, c‑kit tyrosine and Rho A/Rho‑associated kinase 2 pathways, which are involved in curcumin‑induced amelioration of inflammation‑related diarrhea and constipation, were explored in the present study. Results from the present study suggested that curcumin has potential therapeutic value for treating intestinal inflammation and inflammation‑related motility disorders.
研究领域:
药理学
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胃肠病学
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炎症
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
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2025
·
IF 6.7
Tianyuan Bian,Shitao Gao,Xinyu Sun,Songtao Zhou,Yufeng Pei,Jie Song
G-quadruplexes (G4s) are noncanonical DNA structures that regulate gene expression by serving as mechanical barriers. Telomeric DNA and oncogenic promoter regions (e.g., c-KIT and h-RAS) often contain multiple G-quadruplex structures, which may regulate the transcription of adjacent genes through their interactions. These G4 clusters hold significant therapeutic potential as drug targets for cancer treatment. Thus, understanding the interactions within G4 clusters is of considerable importance. However, current bulk measurements struggle to elucidate the dynamic interactions among multiple intrastranded G4s due to the presence of potential interstrand interactions. Herein, we establish a single-molecule platform to investigate the intrastrand interactions between G4s in the c-KIT promoter region. Our results reveal that these G4 units interact to form higher-order structures, with kit* acting as a key regulator that couples and influences the formation and stability of adjacent G4 units. This single-molecule platform can also detect interactions between small-molecule ligands and consecutive G4 clusters. Compared to CX-5461, PDS is a more effective inhibitor of c-KIT. This single-molecule detection method provides robust support for investigating the intrastrand interactions between consecutive G4 structures.
研究领域:
分子生物学
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生物物理学
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药理学
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DOI
PubMed
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
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2018
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IF 3.849
He Yang,Weiqi Wei,Shubin Wu,Junping Zhuang
During the upgrading into biorefinery factories, the traditional pulp and paper mills urgently need to broaden the applications of pulp to achieve higher economic benefits. The conversion of pulp into high value-added bulk chemicals is considered as a potential approach. The pulp was previously treated by HCl and spray drying to decrease its particle size to 71.4 μm as the catalytic raw material. Mesoporous WO 3 with the specific surface area of 58.6 m 2 /g was then synthesized via KIT-6 silica template and it shown high performance in catalytic conversion of pretreated pulp into ethylene glycol. The feedstock conversion was 100% and the yield of ethylene glycol was 62.7% under the optimum condition. In comparison tests, the pretreated pulp and mesoporous WO 3 both displayed better catalytic performance than untreated pulp and nano-sized WO 3 without porous structure. However, the catalytic performance of mesoporous WO 3 decreased in recycling test and the reasons were analyzed preliminarily. This work demonstrated that the ordered mesoporous WO 3 was a high-performance catalyst and had potential for producing ethylene glycol in good cooperation with acid pretreated pulp.
研究领域:
化学工程
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生物质转化
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催化材料
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产品关联
DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
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2021
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IF 10.588
Yuqing Qin,Aroosha Faheem,Yonggang Hu
The excess residues of fluoride ions cause serious human health problems, making their detection highly valuable. In this work, a whole-cell-based biosensor was presented for the detection of fluoride ions, which can inhibit the color reaction of 3,3′,5,5′,-tetramethylbenzidine (TMB) catalyzed by the CotA-laccase of spore surface. This reaction for the detection of fluoride ions could be read out through UV–vis spectrophotometer, smartphone, or standard colorimetric card within 10 min. Under optimum conditions, a linear range of 1–600 μmol L –1 with a detection limit of 0.12 μmol L –1 (3σ/k) was achieved for fluoride ions detection by using UV–vis spectrophotometer. The biosensor coupling with smartphone had a good linear response to fluoride ions concentration in the range of 5–600 μmol L –1 with LOD of 0.90 μmol L –1 (3σ/k). The standard colorimetric card can be directly used for recognizing the fluoride ions level via naked-eyes. A portable kit based on a colorimetric card and smartphone was developed and has been successfully applied for fluoride ions monitoring in surface waters and groundwater. This developed method has several advantages such as rapid, outstanding selectivity and anti-interference, low-cost, ease of operation and storage, and eco-friendliness, meeting the demands of point-of-care testing of fluoride ions and disease prevention.
研究领域:
环境科学
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分析化学
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生物技术
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL SCIENCES
·
2015
·Vol. 31
·期 2
·113-118
·
IF 1.394
Guoyan LIU,Huipeng REN,Yuyu GUAN,Ronghua DAI,Chunyan CHAI
A fast and simple mercury detection kit was developed based on melamine-functionalized gold nanoparticles (GNPs). The detection kit contained reagent 1 (GNPs), reagent 2 (melanine), a reaction cuvette with four separated cells, a colorimetric card and a plastic pipette. The GNPs were prepared by a citrate reduction of HAuCl4. A proper amount of melamine was applied to functionalize the GNPs. The complex reaction took place in the present of Hg2+ in the test samples, leading to the combination of Hg2+ with the C=N group of melamine located on the surface of the GNPs. This reaction resulted in damage to the stability of colloid gold, and the aggregation of GNPs occurred. Different color changes (from claret-red to lilac, purple and plum) were displayed with different concentrations of Hg2+ in the test samples. It was very easy and convenient to determine the amount of mercury ion by the naked eye. The advantages of this methodology are listed as follows: a short detecting time (within 10 min), a high specificity (no significant interference was indicated upon adding a certain amount of Cu2+, Pb2+, Zn2+, Mg2+, Cd2+ and Fe2+), high sensitivity with a detection limit of 0.01 mg L−1 , easy operation and practical on-site use.
研究领域:
环境科学
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纳米生物学
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Jiajing Xia,Binxv Wei,Wenqian Mao,Weizhong Cao,Weizheng Wan,Yuerang Zhang,Dan Xiang,Min Hu
Glucose monitoring is a critical aspect of healthcare, particularly for the clinical diagnosis and ongoing management of diabetes mellitus. However, existing glucose assays still encounter problems such as time-consuming procedures, operational complexity, and reliance on specialized equipment. Our study introduced an innovative point-of-care testing (POCT) platform for one-step glucose detection that integrated a hydrogel kit with a nanozyme-based cascade reaction, which provides significant signal amplification for high sensitivity, and smartphone technology. The nNO 2 -MIL-101-Fe (Fe-MOF), synthesized using the electron-withdrawing ligand 2-nitroterephthalic acid, exhibited catalytic activity comparable to that of enzymes. When glucose oxidase (GOx) was immobilized onto this material, it formed a cascade catalytic system that stabilized enzymes and minimized the diffusion resistance of intermediates. The hydrogel, in its moldable form, acted as a carrier and improved enzyme stability due to its hydrophilic environment. In the cascade catalysis process, glucose from the sample triggered a reaction that converted 3,3′,5,5′-Tetramethylbenzidine (TMB) into a blue product, enabling a direct visual readout using a smartphone. Consequently, this portable POCT platform demonstrated high sensitivity and selectivity for glucose detection with a linear ranging from 0.05 to 1 mM and a detection limit of 0.035 mM. Validation in human serum samples confirmed its excellent accuracy, with recoveries ranging from 95.7 % to 101.6 %. The portable hydrogel kit-based POCT platform has the potential to significantly enhance diabetes management by providing a cost-effective, user-friendly, and accessible glucose monitoring solution.
研究领域:
生物医学工程
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糖尿病技术
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生物传感器
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即时检测
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纳米材料
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产品关联
DOI
高影响因子
JOURNAL OF URBAN HISTORY
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2010
·
IF 0.165
Richard Harris
Urban historical scholars have neglected smaller urban centers, including their residential environments and the forces that shaped them. For a time, one of these forces was the mail-order kit home. Kit manufacturers sold houses to families throughout the United States and Canada but enjoyed their greatest success in small towns where detached single-family homes were the norm. They worked to insert themselves into local building industries: They challenged lumber dealers and ignored architects but strove to mollify the contractors on whom they and their customers depended. They attracted considerable attention and met with initial success: Emerging rapidly after 1905, they had hit their stride by 1914 and enjoyed a heyday in the 1920s. They were stricken by, and failed to recover from, the Depression in large part because lumber dealers had learned how to compete with them.
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DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Zhengang Zhou,Junjie Zheng,Lingyu Shao,Xiao Zhang,Chengjin Pan,Xiuqi Hu,Kai Feng,Hao Song,Shihan Zhang,Chenghang Zheng,Xiang Gao
Catalytic solvent regeneration has garnered significant attention due to its capacity to lower energy demands in industrial processes, promoting broader adoption of carbon capture systems. While progress has been made in catalyst engineering, creating efficient solid acid catalysts still encounters critical obstacles such as synthesizing mesoporous architectures with extensive surface areas and improving the spatial arrangement of active sites. Herein, this study developed a Ni/Fe@KIT-6 catalyst with ordered porosity and dual metal acid sites, leveraging the three-dimensional cubic pore structure of mesoporous silica to enhance liquid-solid mass transfer. The anchored Ni/Fe clusters synergistically enhanced proton transfer, reducing the activation energy of carbamate decomposition through optimized intermediate adsorption configurations. Catalytic testing revealed a 58.1 % enhancement in CO₂ desorption efficiency alongside a 41.2 % decrease in energy requirements relative to non-catalytic systems. The material maintained stable performance across multiple absorption-desorption cycles (efficiency decline <10 %), linked to the KIT-6 framework's retained structural stability and optimized metal-support bonding. Theoretical calculations revealed that the Ni/Fe dual sites lowered the CO 2 desorption reaction energy barrier, aligning with the enhanced CO₂ desorption efficiency. This synergistic interaction of dual metal acid sites within a high-surface-area architecture highlights viable pathways for scalable and energy-efficient CO₂ desorption, thereby advancing practical strategies for large-scale deployment of carbon capture technologies.
研究领域:
催化化学
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碳捕集与封存
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材料科学
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化学工程
·
环境科学
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产品关联
DOI
高影响因子
ACS Omega
·
2019
·
IF 2.584
Chengxiang Shi,Huan Wang,Qiulin Bi,Liqing Li,Pingchuan Sun,Tiehong Chen
Hierarchically porous silica KIT-6 and SBA-15 mesostructures were successfully synthesized by using a mesomorphous complex of a nonionic triblock copolymer (pluronic P123) and an anionic polyelectrolyte (polyacrylic acid) as the dynamic template. The obtained mesoporous silica materials possessed both ordered mesopores (∼7 nm) and nanopores (∼15–50 nm), and the long-range order of the mesophase was not perturbed by the embedded larger secondary nanopores. Moreover, hierarchically porous silica KIT-6 exhibited enhanced adsorption capacity in enzyme and protein immobilization, which was attributed to the hierarchically porous structure.
研究领域:
材料科学
·
生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）