SENSORS AND ACTUATORS B-CHEMICAL
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2022
·
IF 9.221
Yuanyuan Liu,Bangyan Liu,Lian Xia,Hongyan Yu,Qiaodie Wang,Yuangen Wu
Although methods based on mass spectrometry or immunoassays can meet the demands of paraquat detection, their application is limited by a lack of convenience or stability. Herein, a facile visual aptamer-based chromatographic strip (CS) was fabricated for the rapid paraquat detection using the cationic polyelectrolyte poly(diallyl dimethyl ammonium chloride) (PDDA) as a powerful capture molecule at test-line (T-line). The PDDA could capture the gold nanoparticles (AuNPs) through strong electrostatic interactions for visual detection. The proposed CS showed an excellent response for paraquat in the range of 20–150 μg·L −1 , and the limit of detection (LOD) was as low as 4.28 μg·L −1 . This detection method has good stability and specific selectivity , and can visually determine paraquat residues more than 30 μg·L −1 by naked eye within 3 min. The results demonstrated that the prepared CS based on aptamer and cationic polyelectrolyte is an accurate and practicable tool for rapid analysis of pesticide residues .
研究领域:
分析化学
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环境科学
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农业科学
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产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2023
·Vol. 190
·期 10
·1-9
·
IF 5.7
Yang Weiqing,Li Dandan,Li Yunlong,Zheng Yuan,Shan Jiajia
Abstract A method is proposed to fabricate a novel capillary surface-enhanced Raman scattering (SERS) substrate integrating sampling and detection based on meniscus evaporation self-assembled technology, named Meniscus@AgNPs@Capillary substrate. Ag nanoparticles (AgNPs) were arranged in the inner wall of the capillary through meniscus evaporation. The parameters which might affect the deposition of AgNPs during evaporation were investigated, including the evaporation temperature, self-assembly time, the ratio of silver sol to ethanol, and capillary length. The enhancement effect of SERS under different fabrication conditions was investigated using rhodamine 6G (R6G) as a Raman probe. Moreover, the optimal fabricated Meniscus@AgNPs@Capillary substrate was applied to the detection of several environmental pollutants such as polystyrene nanoplastics (PSNPs) and various antibiotics, with limits of detection (LOD) of 10 µg/L and 1 µg/L, respectively. The Meniscus@AgNPs@Capillary substrate presented the advantages of time and effort saving, high sensitivity, and on-site sampling and testing. Graphical Abstract
研究领域:
材料科学
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分析化学
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环境科学
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产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2024
·
IF 10.7
Yi Liu,Huanjiao Weng,Zhiwei Chen,Ming Zong,Shubin Fang,Zili Wang,Shaohua He,Yangming Wu,Jizhen Lin,Shangyuan Feng,Duo Lin
The Omicron variants of SARS-CoV-2 have been spreading globally and has never disappeared from our sight, indicating that its coexistence with humans has become a fact, and monitoring its evolution and spread remains a current task. Although polymerase chain reaction (PCR) is the most commonly used virus detection method, it requires labor-intensive and time-consuming procedures in a laboratory setting. Herein, a multichannel nanoplasmonic sensing chip based on surface enhanced Raman spectroscopy (SERS) was developed for detecting N and S proteins, as well as IgG and IgM, related to SARS-CoV-2 Omicron variants. Through a self-screening process, specific antibodies for on-site and rapid identification of important variants of concerns (VoCs) were obtained, and their binding was confirmed by protein structure analysis. The use of these S protein specific antibodies can accurately identify Omicron VoCs (BA. 5, BF.7，XBB.1.5) with the detection limit (LoD) at 0.16 pg/mL. Then, the proposed SERS array chip was integrated with a hand-held Raman spectrometer to successfully detect the Omicron subvariants in real saliva samples within only 20 minutes, greatly reducing the detection time of PCR. This sensing technology will provide a powerful and rapid point-of-care testing (POCT) method for virus diagnosis, subtype identification, and post infection antibody level monitoring.
研究领域:
生物医学工程
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分析化学
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病毒学
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医学诊断
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2020
·
IF 7.1
Ling Chen,Yuan-Kui Leng,Bin Liu,Juan Liu,Sheng-Peng Wan,Tao Wu,Jinhui Yuan,Liyang Shao,Guoqiang Gu,Yong Qing Fu,Hengyi Xu,Yonghua Xiong,Xing-Dao He,Qiang Wu
An ultra-high sensitivity label-free optical fiber biosensor for inactivated Staphylococcus aureus ( S. aureus ) detection is proposed and investigated in this study, with additional advantages of robust and stability compared to traditional tapered fiber structure. The proposed fiber biosensor is based on a tapered singlemode- no core-singlemode fiber coupler (SNSFC) structure, where the no core fiber was tapered to small diameter (taper-waist diameter of about 10 μm) and functionalized with the pig immunoglobulin G (IgG) antibody for detection of S. aureus . The measured maximum wavelength shift of the sensor for an S. aureus concentration of 7 × 10 1 CFU/mL (colony forming unit per milliliter) is 2.04 nm, which is equivalent to a limit of detection (LOD) of 3.1 CFU/mL (a highest LOD reported so far for optical fiber biosensors), considering the maximum wavelength variation of the sensor in phosphate buffered saline (PBS) is ±0.03 nm over 40 min, where 3 times of maximum wavelength variation (3 × 0.03 = 0.09 nm) is defined as measurement limit. The response time of the developed fiber sensor is less than 30 min. The ultra-sensitive biosensor has potential to be widely applied to various areas such as disease, medical diagnostics and food safety inspection.
研究领域:
生物传感
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光纤技术
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微生物学
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食品安全
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医学诊断
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产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Haoyu Wang,Shiting Chen,Jiqing Liu,Wanyue Liu,Wei Chen,Lin Huang,Chao Yan
In recent years, RPA-CRISPR/Cas12a-based electrochemical biosensors have emerged as highly effective tools for nucleic acid detection. However, the random trans -cleavage characteristics of Cas12a on single-stranded nucleic acid sequences can result in the presence of uncertain nucleic acid residues on the electrode surface post-reaction. This situation complicates subsequent sensitization strategies. In this study, we devised a universal sensitization protocol mediated by terminal deoxynucleotidyl transferase (TdT) , a template-independent DNA polymerase and G-rich spherical nucleic acids (G-rich SNAs) with superior performance. Initially, Cas12a was activated by the specific RPA amplification product and cleaved the closed single stranded DNA (C-ssDNA) on the electrode surface. Then, the TdT enzyme extended residual single stranded DNA (R-ssDNA) on the electrode surface, forming G-Quadruplex structures. Subsequently, G-rich SNAs were immobilized on the electrode surface via π-π stacking interactions with the G-quadruplexes. Consequently, a substantial accumulation of hemin occurred within the G-quadruplex cluster structures, generating significant electrochemical signals. We applied this method to the trace detection of Staphylococcus aureus ( S. aureus ) and achieved a good linear calibration curve with concentrations ranging from 0 CFU/mL to 10 4 CFU/mL. Notably, the limit of detection (LOD) was as low as 1.29 CFU/mL, surpassing that of most similar sensors. These results demonstrate that our proposed universal sensitization method effectively addresses the limitations associated with the random trans -cleavage characteristics of Cas12a.
研究领域:
生物传感
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纳米技术
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分子生物学
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电化学
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产品关联
DOI
高影响因子
TALANTA
·
2019
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IF 4.916
Mengke Wang,Junyang Chen,Dandan Su,Guannan Wang,Xingguang Su
In this paper, a split aptamer based fluorescence resonance energy transfer (FRET) platform was constructed for the determination of adenosine deaminase (ADA) activity by using gold nanoclusters (AuNCs) and gold nanoparticles (AuNPs). A single adenosine triphosphate (ATP) aptamer was split into two fragments (referred to as P1 and P2). P1 was covalently attached to the AuNCs at the 5′ end (P1-AuNCs), and P2 was labeled with AuNPs at the 3′ end (P2-AuNPs). In the presence of ATP, ATP bound with the two fragments with high affinity to link P1-AuNCs and P2-AuNPs together, thus the fluorescence of P1-AuNCs was quenched via FRET from P1-AuNCs to P2-AuNPs. With the addition of ADA, ATP was transformed into inosine triphosphate (ITP), and then P1 and P2 were released to cause the fluorescence recovery of the system. So a split aptamer based FRET platform for ADA detection can be established via the fluorescence intensity change of the system. This platform showed a good linear relationship between the fluorescence intensity and ADA concentration in the range of 2–120 U L −1 , and the limit of detection (LOD) was 0.72 U L −1 . Moreover, the detection of ATP in human serum sample demonstrated the accuracy and applicability of the method for ADA detection in real sample.
研究领域:
生物化学
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分析化学
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纳米技术
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分子生物学
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2023
·
IF 8.4
Xin Liu,Alei Dang,Tiehu Li,Yiting Sun,Weibin Deng,Tung-Chun Lee,Yong Yang,Amir Zada,Boning Wang,Yuhui Liu,Shaoheng Wu,Tingkai Zhao
Sensors integrated with sampling and analysis processes are the key in analytical chemistry , environmental protection and national security. However, achieving reliable and sensitive large-scale rapid screening of target analytes is still challenging. Herein, we demonstrate a robust surface-enhanced Raman scattering (SERS) sensor by constructing dense and uniform plasmonic hotspots on the flexible Ti 3 C 2 Tx MXene/graphene oxide (MG) fibers through a self-assemble strategy at the oil-water interface. The produced SERS substrates exhibited an ultralow limit of detection (LOD, 1 ×10 −15 M for R6G), outstanding sensitivity (EF = 1.53 ×10 12 ) and high stability for R6G molecules (RSD = 9.47%, over 60 days storage). SERS experiments and theoretical simulations suggested that acidified Ti 3 C 2 Tx MXene further excited charge-transfer (CT) resonance of the system, while the assembled Ag nanostructures produced a large electromagnetic (EM) field enhancement. Thus, the fiber sensor not only realizes the multiplexed and powerful detection of pesticide residues (LOD of 10 −11 M) with an error < 7.3% via an established concentration-dependent standard color barcodes, but also allows the recognization of target molecules in a wide range of fields, such as methylene blue , crystal violet and nikethamide. This work provides new insights in the development of versatile label-free sensors for the rapid multiplexed analysis of target molecules in real samples.
研究领域:
分析化学
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环境保护
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国家安全
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材料科学
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纳米技术
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产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 11
·1-11
·
IF 5.3
Xu Yuanyuan,Yin Qingqing,Du Ningjing,Yi Yinhui,Zhu Gangbing
By preparing Cu@CuO aerogel as a nanozyme which exhibits prominent peroxidase-like (POD) activity, an innovative homogeneous electrochemistry (HEC) coupled with the colorimetry dual-model sensing strategy is proposed to detect perfluorooctane sulfonate (PFOS) for the first time. Cu@CuO aerogel accelerates the oxidation process of colorless o-phenylenediamine to form yellow 2,3-diaminophenazinc (DAP), and meanwhile, DAP as an electroactive substance creates a reduction peak current upon the electrochemical measurements. Interestingly, in the presence of PFOS, the POD activity of Cu@CuO aerogel is inhibited since the specific coordination between PFOS and Cu(II) can cover the active sites, resulting in the color of the sensing system becoming light and the peak current of DAP decreasing. This innovative dual-mode detection method showed excellent electrochemical detection of PFOS in the concentration range 10.0 ~ 1125.0 nM with a limit of detection (LOD) as low as 3.3 nM and a LOD of 20.8 nM in the colorimetric detection in the range 62.3 ~ 875 nM. Furthermore, the sensor was successfully used for the analysis of real samples with an RSD value ≤ 6.5%. The successful application of this two-mode sensing method for the determination of PFOS holds promise for the detection of other contaminants in the future. Graphical Abstract
研究领域:
分析化学
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环境科学
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纳米技术
·
传感器
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产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 11
·1-9
·
IF 5.3
Zhang Shengyuan,Yang Ying,Yang Dezhi,Yang Yaling
A metal–organic-framework (MOF) fluorescent sensor is reported based on NH 2 -MIL-101(Fe) propelled pesticide and alkaline phosphatase (ALP) catalytic reaction. Different from previous reports, a cascade reaction system combined with MOF structural changes to generate fluorescence was employed. The rationale is that ALP can hydrolyze L-ascorbic acid 2-phosphate (AAP) into L-ascorbic acid (AA), which can reduce Fe 3+ to decompose structurally NH 2 -MIL-101(Fe), resulting in 2-aminoterephthalic acid (NH 2 -BDC) with intense fluorescence. The fluorescence can be decreased to different degrees due to inhibition of organophosphate pesticides (OPPs) on the activity of ALP. By taking chlorpyrifos (CPY) as the model compound of an OPP pesticide and adding ALP and CPY into the NH 2 -MIL-101(Fe) framework, the resulting cascade reaction fluorescence sensors exhibit a good sensitivity for CPY and ALP sensing. The working ranges are 0.02–2 μg/L and 0.2–20 mU/mL with detection limits (LOD) of 5.31 ng/L and 0.05 mU/mL, respectively. The proposed sensor has been actually applied to satisfactory detection of CPY and ALP in food and serum samples. This fluorescence-based assay may extend the application of MOF-based biosensors. Graphical Abstract
研究领域:
分析化学
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环境科学
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生物化学
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材料科学
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产品关联
DOI
PubMed
高影响因子
Nanomaterials
·
2024
·Vol. 14
·期 12
·1046
·
IF 4.4
Xiangyang Li,Hean Qian,Jin Tao,Mingshuo Cao,Meng Wang,Wenlei Zhai
Extensive use of pesticides in agricultural production has been causing serious health threats to humans and animals. Among them, phorate is a highly toxic organophosphorus insecticide that has been widely used in planting. Due to its harmful effects on human and animal health, it has been restricted for use in many countries. Analytical methods for the rapid and sensitive detection of phorate residues in agricultural products are urgently needed. In this study, a new method was developed by combining surface-enhanced Raman spectroscopy (SERS) and immunochromatography assay (ICA). Hybrid magnetic Fe3O4@Au@DTNB-Ab nanoprobes were prepared by modifying and growing Au nanoseeds on an Fe3O4core. SERS activity of the nanoprobe was optimized by adjusting the concentration of the Au precursor. A rapid and sensitive assay was established by replacing the traditional colloidal gold-based ICA with hybrid SERS nanoprobes for SERS-ICA. After optimizing parameters including coating antibody concentrations and the composition and pH of the buffer solution, the limit of detection (LOD) for phorate could reach 1 ng/mL, with a linear range of 5~100 ng/mL. This LOD is remarkably lower than the maximum residue limit in vegetables and fruits set by the Chinese government. The feasibility of this method was further examined by conducting a spiking test with celery as the real sample. The result demonstrated that this method could serve as a promising platform for rapid and sensitive detection of phorate in agricultural products.
研究领域:
分析化学
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环境科学
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纳米技术
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农业科学
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食品安全
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产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2021
·Vol. 13
·期 14
·1757-1765
·
IF 2.896
Can Zhang,Xiaoxiao Wu,Dongyang Li,Jinnuo Hu,Debin Wan,Zhen Zhang,Bruce D. Hammock
As a major metabolite of pyrethroid pesticides, 3-phenoxybenzoic acid (3-PBA) can be an indicator of health risk and human exposure assessment. Based on nanobodies (Nbs), we have developed a rapid flow-through dot enzyme linked immunosorbent assay (dot ELISA) and gold nanoparticle (GNP) lateral-flow immunoassay for detecting 3-PBA. The limit of detection (LOD) values for detecting 3-PBA by flow-through dot ELISA and GNP lateral-flow immunoassay were 0.01 ng mL−1 and 0.1 ng mL−1, respectively. The samples (urine and lake water) with and without 3-PBA were detected by both nanobody-based flow-through dot ELISA and GNP lateral-flow immunoassay, as well as liquid chromatography-mass spectrometry (LC-MS) for validation. There was good consistency between the results of the immunoassays. This demonstrated that the two developed nanobody-based immunoassays are suitable for rapid detection of 3-PBA.
研究领域:
环境科学
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分析化学
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免疫学
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纳米技术
✓ 已验证
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2021
·
IF 10.618
M.R. Ali,M.S. Bacchu,M.A.A. Setu,S. Akter,M.N. Hasan,F.T. Chowdhury,M.M. Rahman,M.S. Ahommed,M.Z.H. Khan
Due to the epidemics of emerging microbial diseases worldwide, the accurate and rapid quantification of pathogenic bacteria is extremely critical. In this work, a highly sensitive DNA-based electrochemical biosensor has been developed to detect Vibrio cholerae using gold nanocube and 3-aminopropyltriethoxysilane (APTES) modified glassy carbon electrode (GCE) with DNA carrier matrix. Electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV), Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM) experiments were performed to interrogate the proposed sensor at each stage of preparation. The biosensor has demonstrated high sensitivity with a wide linear response range to target DNA from 10 −8 to 10 −14 ( R 2 = 0.992 ) and 10 −14 to 10 −27 molL −1 ( R 2 = 0.993 ) with a limit of detection (LOD) value of 7.41 × 10 −30 molL −1 ( S/N = 5 ). The biosensor also exhibits a selective detection behavior in bacterial cultures that belong to the same and distant genera. Moreover, the proposed sensor can be used for six consecutive DNA assays with a repeatability relative standard deviations (RSD) value of 5% ( n = 5). Besides, the DNA biosensor shows excellent recovery for detecting V. cholerae in poultry feces, indicating that the designed biosensor could become a powerful tool for pathogenic microorganisms screening in clinical diagnostics, food safety, and environmental monitoring.
研究领域:
微生物学
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电化学
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生物技术
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环境科学
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临床诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）