Journal of Future Foods
·
2026
·
IF 10.6
Wang-Wei Zhang,Xiu-Xiu Zhang,Yuan Song,Run-Hui Ma,Zhi-Jing Ni,Kiran Thakur,Jian-Guo Zhang,Yi-Long Ma,Zhao-Jun Wei
Oral administration of probiotic bacteria represents a promising strategy for the prevention and alleviation of colitis; however, its translational potential is frequently constrained by low bacterial viability under gastrointestinal conditions, inadequate mucosal adhesion, oxidative stress, and suboptimal host–microbe interactions at inflamed mucosal sites. To address these limitations, we developed a bilayer nanoencapsulation system for riboflavin-producing L. plantarum B2, consisting of an inner gallic acid–Ca²⁺ metal–polyphenol network and an outer coating of either hyaluronic acid (HA) or bovine serum albumin (BSA). Successful formation of uniform, conformal nanocoatings on the bacterial surface was confirmed by transmission electron microscopy (TEM), scanning electron microscopy (SEM), atomic force microscopy (AFM), zeta potential and particle size analyses, and laser scanning confocal microscopy (LSCM). Among the coated formulations, HA-coated L. plantarum B2 (H-G- L. plantarum B2) exhibited markedly improved storage stability, enhanced resistance to simulated gastrointestinal fluids, and greater tolerance to hydrogen peroxide-induced oxidative stress, while largely preserving bacterial growth kinetics, acidification capacity, riboflavin production, and isoflavone biotransformation activity during soymilk fermentation. Furthermore, HA-based nanoencapsulation significantly enhanced bacterial adhesion to Caco-2 intestinal epithelial monolayers in vitro and prolonged gastrointestinal retention in mice. In a dextran sulfate sodium (DSS)-induced colitis model, oral administration of nanoencapsulated L. plantarum B2 attenuated body weight loss, colon shortening, histopathological injury, and colonic oxidative stress. Mechanistically, the treatment suppressed the levels of TNF-α, IL-1β, calprotectin, MPO, and MDA; elevated IL-10, CAT, and reduced GSH; restored the expression of tight junction proteins ZO-1 and occludin; and modulated gut microbiota composition. These findings establish bilayer nanoencapsulation as an effective and biocompatible strategy to enhance probiotic oral delivery and highlight the therapeutic potential of riboflavin-producing L. plantarum B2 as a microbiome-targeted intervention for colitis management.
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高影响因子
Biology-Basel
·
2026
·Vol. 15
·期 2
·153
·
IF 3.5
Guangxu Zhu,Yunhe Zhao,Yunyan Wang,Baohang Huang,Rongkun Chen,Xingyun Zhao,Panpan Wu,Qiang Tu
Simple SummaryPb2+and Cd2+contamination in water threatens ecosystems and human health. To develop efficient, eco-friendly adsorbents and valorize industrial by-products (red mud, distiller’s grains), this study synthesized a novel ternary composite biochar (RM) by immobilizing red mud andLactobacillus plantarumon distiller’s grain-derived biochar. Characterized via SEM-EDS, XRD, and FTIR, RM exhibited a superior porous structure. Batch adsorption experiments indicated that the adsorption on RM followed pseudo-second-order kinetics, with Cd2+adsorption fitting the Langmuir model and Pb2+fitting the Freundlich model. Key mechanisms included surface complexation, ion exchange, and coprecipitation. RM integrates the synergistic advantages of minerals, microorganisms, and biochar, offering a promising, sustainable solution for heavy metal-contaminated water remediation.Pb2+and Cd2+represent common heavy metal contaminants in aquatic environments, posing significant risks to ecosystem stability and human health. To develop efficient adsorbents for removing Cd2+and Pb2+while achieving resource utilization of industrial by-products (red mud and distiller’s grains), this study synthesized a novel composite biochar—red mud–Lactobacillus plantarumcomposite biochar (RM)—by immobilizing red mud andLactobacillus plantarumonto biochar derived from distiller’s grains. The structural and chemical properties of RM were characterized using SEM-EDS, XRD, and FTIR. Batch adsorption experiments were conducted to evaluate the effects of various experimental factors on Cd2+and Pb2+adsorption. The adsorption process was further elucidated through kinetic and isothermal models, revealing that it follows the pseudo-second-order kinetic model. Equilibrium data were best described by the Langmuir model for Cd2+and the Freundlich model for Pb2+. The maximum adsorption capacities reached 12.13 mg/g for Cd2+and 130.10 mg/g for Pb2+. The primary mechanisms involved in Cd2+and Pb2+adsorption by RM include surface complexation, cation–π interactions, ion exchange, and coprecipitation. These findings demonstrate that RM represents a promising and effective adsorbent for the remediation of heavy metal-contaminated water.
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PubMed
高影响因子
JOURNAL OF APPLIED MICROBIOLOGY
·
2020
·Vol. 128
·期 6
·1764-1775
·
IF 3.066
Zheng L.,Hu Y.,He X.,Zhao Y.,Xu H.
AimsThe purpose of this study was to isolate Lactobacillus from gastrointestinal tract of healthy postweaning piglets and investigate its synergistic antimicrobial and probiotic effects with ZnO nanoparticles (nZnO).Methods and ResultsOf the 128 isolates, Lactobacillus plantarum BLPL03 was selected based on its excellent acid and bile salt tolerance properties. Lactobacillus plantarum BLPL03 was sensitive to β‐lactams, macrolides, amphenicols and cephalosporins, whereas it displayed the steady resistance to aminoglycosides, tetracyclines, quinolones and peptide antibiotics. In vitro analysis of antibacterial activities showed that L. plantarum BLPL03 inhibited the four common food‐borne pathogenic bacteria including Escherichia coli O157:H7 CMCC 44828, Salmonella Typhimurium ATCC 13311, Staphylococcus aureus CMCC 26003 and Listeria monocytogenes CMCC 54007 in synergy with nZnO. Furthermore, the quantitative polymerase chain reaction test demonstrated that the combined administration of L. plantarum BLPL03 fermentation liquor (LFL) and nZnO synergistically elevated the faecal number of Bifidobacterium by 73·19‐fold, and reduced the two potential enteropathogenic bacteria Enterobacteriaceae and Clostridium perfringens in mice challenged with Salm. Typhimurium. Finally, dietary supplementation with low dose of nZnO (20 mg kg−1) when combined with LFL administration enhanced final body weight, fur appearance and average daily gain, and decreased feed conversion ratio and diarrhoea incidence in weaned piglets. The faecal Bifidobacterium and Lactobacillus of piglets were dramatically enhanced by 81·96‐ and 3·15‐fold, respectively, after administration of a mixture of nZnO and LFL. Meanwhile, combination of nZnO with LFL resulted in low levels of Bacteroides, Enterococcus, and Enterobacteriaceae.ConclusionsA combination of nZnO and LFL exhibits potential health‐benefit properties for the control of gut microbial composition by their synergistic antimicrobial and probiotic effects.Significance and Impact of the StudyThis study may provide a potential nutritional strategy to improve performance and gut health of animals with gut microbiota disorders caused by pathogen infections and weanling, and so on.
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高影响因子
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
·
2013
·Vol. 236
·期 5
·817-826
·
IF 1.436
Zhang Hongzhi,Li Wei,Rui Xin,Sun Xingmin,Dong Mingsheng
A novel food-grade strain Lactobacillus plantarum 70810 producing β-galactosidase with high transgalactosylation activity was isolated from Chinese paocai. The galactooligosaccharides (GOS) were synthesized by using this enzyme with a maximum yield of 44.3 % (w/w) from 400 g/L lactose at 45 °C for 10 h. The β-galactosidase from this strain was purified to homogeneity by ammonium sulfate precipitation, anion exchange chromatography and gel filtration chromatography. It was a heterodimer arrangement of approximately 105 kDa composed of two subunits of 35 and 72 kDa. The optimal pH of the purified β-galactosidase was 8.0 for both o -nitrophenyl-β- d -galactopyranoside ( o NPG) and lactose hydrolysis, and optimal temperature was 60 °C and 55 °C, respectively. Its K
m
and V
max values for o NPG and lactose were 0.89 ± 0.05 mM, 194 ± 3.0 μmoL/min/mg protein, and 9.88 ± 0.16 mM, 15.88 ± 0.21 μmoL/min/mg protein, respectively. This enzyme was slightly inhibited by the hydrolysis products, that is, glucose and galactose. Since the β-galactosidase from L. plantarum 70810 exhibited higher transgalactosylation activity, strong affinity for lactose and low end-product inhibition, it was suggested to be a potential candidate for the synthesis of prebiotic GOS.
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LWT-FOOD SCIENCE AND TECHNOLOGY
·
2025
·
IF 6.6
Xiaohan Wang,Shujin Chen,Xirui Zhang,Bin Liang,Xiulian Li,Yan Li,Chanchan Sun
Lactobacillus plantarum ( L. plantarum ) was embedded in pea protein isolation (PPI)/sodium alginate (SA) composite microgels prepared by double crosslinking with transglutaminase (TGase) concentration of 10.0 U/g PTN and calcium chloride (CaCl 2 ) concentrations of 0, 0.05, 0.10, 0.15, 0.20, and 0.25 g/L, respectively. The influence of the CaCl 2 concentration on the structure, physicochemical properties, and functional properties of the composite microgels was investigated to reveal the conformational relationship between the structure and the delivery effect of encapsulated probiotics. The results showed that higher concentrations of CaCl 2 favoured the unfolding and aggregation of proteins through hydrophobic interactions and Ca 2+ bridges, thus increasing gel strength and mechanical properties, as well as enhancing thermal stability. When CaCl 2 was added up to a concentration of 0.20 g/L, the encapsulation efficiency (EE) of microgels reached a maximum of 92.62 ± 1.90%. During in vitro digestion, the resistance of the composite microgels to simulated gastric fluid (SGF) increased with rising CaCl 2 concentration, whereas the number of viable bacteria released in simulated intestinal fluid (SIF) decreased. After 120 minutes of immersion in the intestinal fluid, PS-Ca 2+ 2.0% released the least number of viable bacteria at 4.10×10 8 CFU/g than other double-crosslinked microgels, which can avoid the premature release of probiotics in the small intestine, and was conducive to realize the colon-targeted delivery of probiotics. Overall, the structural and functional enhancements achieved by CaCl 2 addition highlight the potential of PPI-SA composite microgels for probiotic protection and delivery.
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Applied Food Research
·
2025
·
IF 6.2
Jing-Yu Feng,Wang-Wei Zhang,Kiran Thakur,Fei Hu,Jian-Guo Zhang,Zhao-Jun Wei
Okara, a soybean by-product, holds significant industrial potential but is currently limited by its unfavorable physicochemical properties. This study innovatively explores the synergistic effects of dry planetary ball milling and Lactobacillus plantarum UFG10 fermentation on enhancing the nutritional and functional properties of okara. As the ball milling time increased (0–10 h), the particle size of okara significantly decreased. UFG10 fermentation significantly reduced the particle size of okara that was un-milled (UO), milled for 2 h (M2), and milled for 5 h (M5), with the particle sizes decreasing from 899 µm, 588 µm, and 251 µm to 367 µm (UF), 360 µm (F2), and 177 µm (F5), respectively. Fermentation had no significant effect on the zeta potential of okara but significantly improved its rheological properties. Furthermore, this dual treatment significantly improved the functional properties of okara such as water holding capacity, water swelling capacity, oil holding capacity, foaming ability and cation exchange capacity. Fourier transform infrared (FT-IR) spectroscopy and X-ray diffraction (XRD) analyses confirmed structural changes in okara, highlighting the effectiveness of the combined treatment in enhancing its physicochemical properties. The fermentation treatment significantly increased the total phenolic content (with an average increase of over 10-fold) and antioxidant activity of okara, which were positively correlated with the ball milling time. Our findings demonstrate that integrating dry planetary ball milling with fermentation represents a novel and effective strategy to transform okara into a high-quality functional food ingredient, offering new insights for its valorization in the food industry.
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Foods
·
2024
·Vol. 13
·期 11
·1727
·
IF 4.7
Chengyu Lu,Ziwei Chen,Yuvaraj Ravikumar,Guoyan Zhang,Xinrui Tang,Yufei Zhang,Mei Zhao,Wenjing Sun,Xianghui Qi
L-Arabinose isomerase (L-AI) has been commonly used as an efficient biocatalyst to produce D-tagatose via the isomerization of D-galactose. However, it remains a significant challenge to efficiently synthesize D-tagatose using the native (wild type) L-AI at an industrial scale. Hence, it is extremely urgent to redesign L-AI to improve its catalytic efficiency towards D-galactose, and herein a structure-based molecular modification ofLactobacillus plantarumCY6 L-AI (LpAI) was performed. Among the engineered LpAI, both F118M and F279I mutants showed an increased D-galactose isomerization activity. Particularly, the specific activity of double mutant F118M/F279I towards D-galactose was increased by 210.1% compared to that of the wild type LpAI (WT). Besides the catalytic activity, the substrate preference of F118M/F279I was also largely changed from L-arabinose to D-galactose. In the enzymatic production of D-tagatose, the yield and conversion ratio of F118M/F279I were increased by 81.2% and 79.6%, respectively, compared to that of WT. Furthermore, the D-tagatose production of whole cells expressing F118M/F279I displayed about 2-fold higher than that of WT cell. These results revealed that the designed site-directed mutagenesis is useful for improving the catalytic efficiency of LpAI towards D-galactose.
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FOOD HYDROCOLLOIDS
·
2025
·
IF 12.4
Yejun Zhong,Yazhe Wang,Jianyong Wu,David Julian McClements,Wenrong Huang,Liqiang Zou,Er Sheng Gong
Encapsulation technology is a crucial method for enhancing probiotic survival through gastrointestinal digestion. This study utilized ethanol-modified pomelo pith matrix (PP) passed through 40-, 100-, and 200-mesh sieves (PP-40, PP-60, and PP-100) and sodium alginate and/or fructooligosaccharide (FOS) to create gel beads for encapsulating Lactobacillus plantarum (LP). The effects of these gel beads on probiotic loading, protection, release behavior, and gut microbiota regulation were investigated. The 100-mesh pomelo pith matrix increased the specific surface area and pore volume, enabling uniform probiotic distribution within gel beads and supporting controlled swelling behavior. Pomelo pith matrix gel beads, particularly PP-LP-100, exhibited superior acid tolerance, controlled release, higher loading capacity, improved survival during simulated digestion, and greater protection during pasteurization and storage for Lactobacillus plantarum . Incorporating FOS into pomelo pith matrix gel beads boosted microbial fermentation and diversity, leading to increased gas production, total SCFA levels, and butyric acid content. The gel beads containing FOS increased the abundance of Bifidobacterium , Blautia , Faecalibacterium , and Ruminococcus , while PP-LP gel beads enriched Bacteroides and Lactobacillus . This study provides a foundation for developing advanced probiotic formulations with improved stability and controlled release.
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高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2025
·
IF 6.6
Mengchen Li,Bo Li,Binbin Long,Leibin Li,Yang Chen,Mingye Peng,Mengzhou Zhou
Lactobacillus plantarum R is the predominant bacterium widely used in food fermentation. However, it has a low tolerance to ethanol during fermentation, which restricts its broader application in the food industry. Although biofilm formation enhances bacterial resistance to various stresses, regulating biofilm formation in L. plantarum R remains challenging. In this study, we established a pathway for producing autoinducer 2 (AI-2), a quorum-sensing (QS) signaling molecule, by heterologously expressing pfs and LuxS in L. plantarum R. This approach aimed to modulate cell membrane structure and improve bacterial ethanol resistance. The findings showed that exogenous AI-2 significantly enhanced the growth of L. plantarum R under high-ethanol concentration and improved its biofilm formation at different ethanol concentrations. Furthermore, a multi-omics analysis revealed potential mechanisms by which AI-2 improved the tolerance of L. plantarum R to ethanol. Notably, the upregulation of the crr gene, a component of the phosphotransferase system (PTS), enhanced biofilm formation in response to ethanol stress. In addition, differential metabolites enriched in cysteine and methionine metabolic pathways further supported the role of QS in enhancing the tolerance of L. plantarum R to ethanol. These findings lay the groundwork for enhancing the fermentation performance of strains applied in the food fermentation industry, presenting a novel method for expanding the utilization of L. plantarum R in high-ethanol fermentation.
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Frontiers in Microbiology
·
2023
·
IF 5.2
Wen Xingjian,Liu Hejing,Luo Xiaoling,Lui Li,Fan Jiuyu,Xing Yajing,Wang Jia,Qiao Xingfang,Li Na,Wang Guixue
Atherosclerosis and non-alcoholic fatty liver disease (NAFLD) have been increasing at an alarming rate worldwide. Many clinical studies have underlined the link between NAFLD and atherosclerosis. Our previous experiments have discovered that Lactobacillus (L.) plantarum ATCC14917 supplementation could decrease the progression of atherosclerotic lesion formation. In this study, we aimed to investigate the role of supplementation of L. plantarum ATCC14917 mitigates liver injury in rats fed with a high-fat diet (HFD, 45% kcal from fat). A total of 32 rats were randomly divided into four groups, including two intervention groups, who fed with HFD and administering either 1 × 107 or 1 × 109 colony forming units (CFU) of L. plantarum ATCC14917, the normal control group, and the HFD control group. The results showed that supplementation with low-dose and high-dose of L. plantarum ATCC14917 for 8 weeks could alleviate the body weight gain (p < 0.05), hepatic steatosis, and serum lipid metabolism (p < 0.05) in HFD-fed rats. Moreover, supplementation of L. plantarum ATCC 14917 decreased total cholesterol (TC), triglyceride (TG), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) levels (p < 0.05) in serum, and improved HFD-associated inflammation (p < 0.05). Furthermore, cecal contents were analyzed by high-throughput 16S ribosomal RNA sequencing. The results indicated that supplementation of L. plantarum ATCC 14917 could ameliorate HFD-induced gut dysbiosis. In summary, our findings suggest that supplementation of L. plantarum ATCC 14917 could mitigate NAFLD in rats, suggesting it may be considered as a probiotic agent for preventing HFD-induced obesity.
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高影响因子
INTERNATIONAL IMMUNOPHARMACOLOGY
·
2022
·
IF 5.714
Hua Han,Lu Liu,Jieping Zhang,Meng Zhang,Xinyu Chen,Yuyuan Huang,Wenxiu Ma,Huanlong Qin,Li Shen,Jundong Zhang,Wensheng Yang
Inflammatory bowel disease (IBD), a progressive and unpredictable colorectal inflammatory disease, is a global health problem. Currently, therapeutic strategies for the management of the disease are limited. Results from our previous studies indicated that probiotic Lactobacillus plantarum exhibits therapeutic effects against IBD, and through screening, we obtained an active 61-amino-acid long protein, L. plantarum membrane protein 1 (LpMP-1). Based on druggability-guided strategies, the search for LpMPs with lower molecular weights and better bioactivities contributes to the development of new anti-inflammatory agents to overcome the limitations of existing therapies against IBD. We used amino-acid-truncation strategies to obtain modified LpMPs (LpMP-2 - LpMP-9) using LpMP-1 as the parent template. Furthermore, we systematically evaluated the anti-colitis pharmacodynamics of these LpMPs in terms of symptomatology, histopathology, and cytokine levels in DSS-induced ulcerative colitis mice. Their possible targets of action against IBD was investigated under an iTRAQ-based pharmacoproteomic system and a docking-guided receptor-ligand relationship frame. We found a new active protein, LpMP-8, which had a lower molecular weight than LpMP-1. LpMP-8 was found to exhibit anti-colitis activity following oral administration in vivo (50 μg/kg) by improving symptoms of colitis, colonic ulcerations, and cytokine disorders. TLRs and TGF-β were found to be involved in the action of LpMP-8 against colitis; LpMP-8 was to compete with TLR4-MD2-bound LPS and reverse TGF-β and Smad2/7 disorders. Our probiotic-derived LpMP-8 was shown to elicit oral anti-colitis activity, and its significant efficacy is probably associated with TLR4 and TGF-β.
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高影响因子
FOOD HYDROCOLLOIDS
·
2024
·
IF 11
Gege Sun,Jinhua Gong,David Julian McClements,Shilin Liu,Bin Li,Yan Li
Water-in-oil-in-water (W/O/W) emulsions are kind of promising vehicles for delivering hydrophilic and hydrophobic components. In this study, the double interfaces of W/O/W emulsion was modified to improve their properties. Specifically, in situ dynamic imine chemistry was used to crosslink the interfaces in W/O/W emulsions. W/O/W emulsions were prepared that contained whey proteins in the inner and outer aqueous phases and polyglyceryl polyricinoleate (PGPR), a hydrophobic emulsifier, in the oil phase. Aldehyde oils were added to the oil phase of the emulsions to chemically react with the proteins in both the inner and outer water phases. The stability, interfacial property and permeability of W/OW emulsions were characterized using microscopy observation, tensiometer and diffusion extent determination. Then, the gastrointestinal protection and delivery of probiotics in W/O/W emulsion was determined. The results showed that the modified W/O/W emulsions possessed high storage stability. The diffusion of Rhodamine B and hydrogen ions between the internal and external aqueous phases was retarded after cinnamaldehyde interfacial modification. Atomic force microscopy images, interfacial rheology analysis and chemical composition analysis confirmed the interaction occurrence between the lipophilic aldehyde and whey protein. After the optimization of aldehyde oils, probiotics encapsulated in W/O/W emulsion exhibited enhanced cell viability during long-term storage and after going through gastric digestion site. Overall, our results provide useful information that may facilitate the design of W/O/W emulsions that can protect probiotics from harsh environments.
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