Canadian Journal of Chemistry
·
2017
·Vol. 95
·期 8
·
IF 1.08
Juan Han,Yun Wang,Tong Chen,Xiaowei Hu,Lei Gu,Xu Tang,Lei Wang,Liang Ni
Heat-induced coacervation of triblock copolymer solution was described, and its application in the purification of Lycium barbarum polysaccharide (LBP) was investigated. The formation of coacervate micelles–protein complex combined with the incompatibility between coacervate micelles and polysaccharide made it an ideal system for the separation of protein and LBP. This separation process was governed by a series of parameters including polymer concentration, amount of crude LBP solution, and pH. In the primary coacervation extraction process, LBP was preferentially distributed to dilute phase with a high recovery ratio of 82%, whereas 87% of protein was partitioned to the coacervate phase. The coacervate micelles–protein interaction and the interphase potential was regulated by temperature and electrolytes, respectively, which contributed to the recovery and recycling of the polymer. After phase separation, LBP was precipitated with the addition of ethanol. The FTIR spectrum was used to identify LBP. In addition, the antioxidant activity of LBP was measured.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2026
·
IF 8.7
Chunlan Zou,Lisi Liang,Mouming Zhao,Lianzhu Lin
The intricate interplay between dietary components, the gut microbiome, and host immunity is a core of intestinal immune research . Lycium barbarum polysaccharide (LBP)-chlorogenic acid (CGA) complex exhibits proliferative activity of Bacteroides and strong inhibitory effect on Staphylococcus aureus, but lacks of the microbial fermentation characteristics and immunomodulatory effect. Herein, in vitro fecal bacteria microbiota and macrophages models were employed to investigated the regulatory capacity of the LBP-CGA complex on intestinal microbiota and immunomodulatory activities of its metabolites. Results showed that LBP and LBP-CGA both readily fermentable. Fermentation led to significant molecular weight and pH reductions, nearly 85% polysaccharide degradation. Notably, LBP-CGA complex was more favorable to increase the relative abundance of Sutterella , Veillonella , Faecalibacterium and contents of SCFAs than LBP. Interesting, LBP fermentation product possessed the potential for immune-enhancing, with the polysaccharide fraction was the key active component. Conversely, LBP-CGA complex fermentation product elicited the potential for immune-suppressive response, with dihydrocaffeic acid of CGA metabolites was critical contributors. The data further indicated LBP-CGA exhibited better immunomodulatory of intestinal microbiota probably due to the proliferation of Bacteroides . Bacteroides may improve the bio-transformation of CGA and produce more dihydrocaffeic acid, which may in turn potentiate the immunosuppressive activity of the LBP-CGA complex fermentation product. To summarize, the results revealed that CGA elevated the ability of LBP to regulate the intestinal microbiota and to potential exert immunosuppression. This study offers profound insights into the development of LBP-CGA complex as a prebiotic with excellent anti-inflammatory effect.
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Food & Function
·
2022
·Vol. 13
·期 16
·8703-8716
·
IF 6.317
Cui Cao,Linlin Wang,Chunqing Ai,Guiping Gong,Zhongfu Wang,Linjuan Huang,Shuang Song,Beiwei Zhu
Ulcerative colitis (UC) is often accompanied by the dysbiosis of gut microbiota and metabolism. Our previous study indicated that arabinogalactan from Lycium barbarum (LBP-3) could markedly attenuate the symptoms of chronic UC in mice by modulating the structure of gut microbiota. This study explored the impact of LBP-3 on the fecal metabolomic profiling of the same cohort of mice by HPLC-TripleTOF/MS. Untargeted metabolomic analyses indicated that supplementation with LBP-3 markedly reversed 18 of the 48 differential metabolites (mainly belonging to amino acids and organic acids) disturbed by DSS. Targeted metabolomics revealed that the lower levels of tryptophan, lysine, diiodothyronine, kynurenine, and betaine and higher levels of phenylalanine, leucine, glutamine, isoleucine, homoserine, (S)-2-hydroxyglutarate, 2-isopropylmalic acid, ascorbic acid, gluconic acid, and taurine, which were caused by DSS induction, were reversed by LBP-3 treatment. In addition, pathway analysis showed that the pentose phosphate pathway, phenylalanine metabolism, ascorbate and aldarate metabolism, and phenylalanine, tyrosine and tryptophan biosynthesis were strongly affected by LBP-3. More importantly, the above amino acids, organic acids, and metabolic pathways changed by LBP-3 were correlated with the abundance of gut microbiota such as Turicibacter, Lactobacillus, Parasutterella, Odoribacter, Veillonella, Faecalibacterium, and Ruminococcaceae. This study advances our understanding of the interaction between the microbiome and metabolomics in DSS-induced chronic colitis after LBP-3 treatment.
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Foods
·
2022
·Vol. 11
·期 3
·306
·
IF 5.561
Tingting Yang,Yuhang Hu,Yamei Yan,Wangting Zhou,Guijie Chen,Xiaoxiong Zeng,Youlong Cao
The fruits ofLycium barbarumare rich in flavonoids, which may contribute to the health-promoting function ofLycium barbarum. However, the composition of flavonoids in the fruits ofLycium barbarum(LBFs) has received little attention. Thus, the goal of this work was to identify more kinds of flavonoids from fruits ofLycium barbarumby liquid chromatography–mass spectrometry. The potential antioxidant and anti-inflammatory activities of LBFs in vitro were also investigated. Thirteen flavonoid compounds were identified in LBFs, of which daphnetin, 6,7-dihydroxycoumarin, astragalin, taxifolin, eriodictyol, naringenin, and chrysoeriol were identified for the first time in the fruits ofLycium barbarum, which greatly enriched the variety of flavonoids in the fruits ofLycium barbarum. LBFs showed a similar superior antioxidant activity to vitamin C. Furthermore, LBFs exhibited an anti-inflammatory activity by suppressing the production of nitric oxide and pro-inflammatory cytokines, including tumor necrosis factor-alpha, interleukin-1β, and interleukin-6, in lipopolysaccharide-treated RAW264.7 macrophage cells. This study demonstrated the potential development of LBFs as functional foods.Keywords:Lycium barbarum;flavonoids;liquid chromatography mass spectrometer (LC-MS);antioxidant;anti-inflammatory
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TALANTA
·
2019
·
IF 4.916
Mohammad Chand Ali,Jia Chen,Haijuan Zhang,Zhan Li,Liang Zhao,Hongdeng Qiu
The efficient extraction of natural products from biomass is deemed an essential area of interest using green technology in the pharmaceutical and biochemical industries. In recent years, deep eutectic solvents (DESs) have been paying broad interest as compatible, safe, and inexpensive solvents. In this study, we addressed, for the first time, to give a fruitful illustration to extract bioactive compounds from favourite Lycium barbarum L. fruits using DESs as green designer solvents, and displaying the tuneability of DESs to extract bioactive compounds from biomass efficiently. Among the studied DESs, the 1 : 2 M mixture of choline chloride and p -toluene sulfonic acid (DESs-6) with an ultra-sound assisted extraction (UAE) method was more efficient to get high extraction yields of flavonoids including myricetin (57.2 mg/g), morin (12.7 mg/g), and rutin (9.1 mg/g) in net DESs. The resulting extraction method in which 50 mg of sample powder was extracted by UAE for 1.5 h using DESs-6 was found to be a more efficient process than conventional extraction methods such as heating plus stirring. Operational conditions including the extractant to sample solid ratio, the effect of water content, the effect of temperature, recycling of DESs, and the effect of ultra-sound irradiation time were investigated. The proposed DESs based extraction procedure attained recovery for flavonoids were in the range 75.6%–96.9%. Therefore, p -toluenesulfonic acid-based DESs may have strong potential as promising materials for the green and efficient extraction of pharmaceutical compounds from other plants or fruits.
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TOXICOLOGY
·
2024
·
IF 4.8
Linjing Xu,Huan Liu,Yifeng Rang,Lizi Zhou,Xukai wang,Yinhuan Li,Chunhong Liu
Nonylphenol (NP) and octylphenol (OP) are environmental contaminants with potential endocrine disrupting effects. However, there is limited research on the mechanisms and intervention of combined NP and OP exposure-induced neurotoxicity. This study aims to explore the cytotoxicity of combined NP and OP exposure and evaluate the potential of Lycium barbarum polysaccharides (LBP) in mitigating the aforementioned toxicity. In present study, LBP (62.5, 125 and 250 µg/mL) were applied to intervene rat adrenal pheochromocytoma (PC-12) cells treated with combined NP and OP (NP: OP = 4:1, w/w; 1, 2, 4 and 8 µg/mL). The results showed that NP and OP induced oxidative stress, disrupted the 5-hydroxytryptamine (5-HT) and cholinergic systems in PC-12 cells. Additionally, they activated the p38 protein kinase (p38) and suppressed the expression of silent information regulation type 1 (SIRT1), monoamine oxidase A (MAOA), phosphorylated cyclic-AMP response binding protein (p-CREB), brain-derived neurotrophic factor (BDNF) and phosphorylated tropomyosin-related kinase receptor type B (p-TrkB). However, N-acetyl-L-cysteine (NAC) treatment counteracted the changes of signalling molecule p38, SIRT1/MAOA and CREB/BDNF/TrkB pathways-related proteins induced by NP and OP. LBP pretreatment ameliorated combined NP and OP exposure-induced oxidative stress and neurotransmitter imbalances. Furthermore, the application of LBP and administration of a p38 inhibitor both reversed the alterations in the signaling molecule p38, as well as the proteins associated to the SIRT1/MAOA and CREB/BDNF/TrkB pathways. These results implied that LBP may have neuroprotective effects via p38-mediated SIRT1/MAOA and CREB/BDNF/TrkB pathways.
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FOOD CHEMISTRY
·
2024
·
IF 8.5
Hailin Wang,Jinrong Zhang,Pingfan Rao,Shaomin Zheng,Guoqiang Li,Huan Han,Ying Chen,Leiwen Xiang
Protein-polysaccharide interactions are crucial for food system structure and stability. This study investigates the interaction of Lycium barbarum polysaccharide (LBP) at 0–2.00 % concentrations with whey protein isolate (WPI), focusing on functionality and structural changes. LBP covalently grafted onto WPI, as confirmed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), forming WPI-LBP complexes with a maximum degree of grafting (DG) of 44.58 % at 2.00 % LBP. This grafting reduced WPI's surface hydrophobicity (H 0 ) and improved solubility, emulsifying properties, and digestibility under certain conditions, with optimal antioxidant activity at 1.00 % LBP. Multispectral analysis and microscopy showed LBP grafting alters WPI's secondary, tertiary, crystalline, and micro/nanostructures. The comprehensive analysis indicates that the interaction between LBP and WPI involves covalent bonding, hydrogen bonding, hydrophobic interactions, and electrostatic forces, as supported by zeta potential and chemical forces results. These findings suggest LBP-protein complexes as promising food materials for enhancing functionality and stability in the food industry.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Tian Liu,Yun Zhang,Ze Wu,Chen-jing Zhao,Xi Dong,He-xin Gong,Bing Jin,Miao-miao Han,Jin-jia Wu,Yi-kai Fan,Nan Li,Ying-xia Xiong,Zi-qian Zhang,Zheng-qi Dong
The macrophage to myofibroblasts transition (MMT) has been reported as a newly key target in renal fibrosis. Lycium barbarum L. is a traditional Chinese medicine for improving renal function, in which its polysaccharides (LBPs) are the mainly active components. However, whether the role of LBPs in treating renal fibrosis is related to MMT process remain unclear. The purpose of this study was to explore the relationship between the regulating effect on MMT process and the anti-fibrotic effect of LBPs. Initially, small molecular weight LBPs fractions (LBP-S) were firstly isolated via Sephadex G-100 column. Then, the potent inhibitory effect of LBP-S on MMT process was revealed on bone marrow-derived macrophages (BMDM) model induced by TGF-β. Subsequently, the chemical structure of LBP-S was elucidated through monosaccharide, methylation and NMR spectrum analysis. In vivo biodistribution characteristics studies demonstrated that LBP-S exhibited effectively accumulation in kidney via intraperitoneal administration. Finally, LBP-S showed a satisfactory anti-renal fibrotic effect on unilateral ureteral obstruction operation (UUO) mice, which was significantly reduced following macrophage depletion. Overall, our findings indicated that LPB-S could alleviate renal fibrosis through regulating MMT process and providing new candidate agents for chronic kidney disease (CKD) related fibrosis treatment.
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LWT-FOOD SCIENCE AND TECHNOLOGY
·
2024
·
IF 6
Huiqiang Hu,Yunlong Mei,Yunpeng Wei,Zhenyu Xu,Yuping Zhao,Huaxing Xu,Xiaobo Mao,Luqi Huang
The bioactive components of goji berries ( Lycium barbarum ) are crucial determinants of their nutritional and commercial value. In this study, we combined hyperspectral imaging technology (HSI) with a one-dimensional convolutional neural network (1DCNN) to predict the content of chemical compositions in goji. To enhance the model's ability to focus on relevant information, we introduced the channel attention module (CAM), spectral attention module (SAM) and fused them together, which can focus on output features of the convolution kernels differently and adaptively emphasize more effective spectral bands, respectively. Moreover, considering the limitations of traditional single-task prediction methods and the inherent correlations among different constituents, we employed a multi-task CNN for the simultaneous prediction of various goji constituents. The results indicate that the attention-enhanced 1DCNN model outperforms both the partial least squares regression (PLSR) model and the vanilla 1DCNN. With multi-task learning, the model achieves optimal performance, achieving an average R 2 of 0.9435 for the prediction of the three components. Our research develops an efficient and accurate method for predicting the constituents of goji berries, providing a valuable, convenient, and effective tool for assessing and detecting the quality of fruit products in the food industry.
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FOOD CHEMISTRY
·
2025
·
IF 9.8
Mengcong Zhang,Li Lu,Xiaowen Hu,Chengzhe Zhou,Cheng Zhang,Jiaxin Fang,Linyuan Duan,Bo Zhang,Yuqiong Guo
Goji ( Lycium barbarum L.) leaf tea (LBLT) has gained popularity among consumers owing to its pleasant aroma. Selecting optimal Goji cultivars for tea processing is critical for enhancing the quality of LBLT. This study investigated the aroma characteristics of Goji leaf white tea (LBLWT) and green tea (LBLGT) prepared from 25 Goji lines. LBLWT emitted woody, floral and fruity aromas, while LBLGT exhibited a distinct roasted corn aroma. The integrated application of variable importance in projection, odour activity value and partial least-squares regression analyses led to the identification of key aroma compounds, whose metabolic pathways were also illustrated. Using multi-criteria evaluation methods, excellent Goji lines suitable for processing LBLWT (Z20, Z48 and N1) and LBLGT (Z85, Z20, N1 and Z68) were screened. This study took the lead in adopting multi-criteria evaluation methods, screening high-quality Goji lines and providing theoretical basis for high-quality LBLT processing.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Tian-yu Liu,Zi-wei Han,Si-si Jia,Kai Ma,Mei Li,Xin Yi,Hua Zhu,Jia-hao Fan,Hong-wei Qiu,Guang-ping Lv,He Huang
Addressing microbial imbalance is a critical therapeutic strategy for ulcerative colitis (UC). Natural polysaccharides have attracted increasing attention for their potential in UC prevention. In this study, we purified and characterized an acidic heteropolysaccharide (LBP 2–1) from Lycium barbarum L. and systematically evaluated its therapeutic effects on UC. Monosaccharide composition analysis combined with methylation analysis and NMR spectroscopy revealed that LBP 2–1 possesses a complex structure. The backbone is →2)-α-L-Rha p -(1 → 4)-α-D-GalA p -(1 → 6)-β-D-Gal p -(1 → and branched at the O-3/O-4 positions of →3,6)-β-D-Gal p -(1 → and →2,4)-α-L-Rha p -(1 →. The branch chains consist of →5)-α-L-Ara f -(1 →, →3,5)-α-L-Ara f -(1 →, →3)-β-D-Gal p -(1 →, β-D-Gal p -(1 →, and α-L-Ara f -(1 →. Particularly, LBP 2–1 relieved the symptoms of weight loss and colon shortening, and reduced DAI and histopathological scores in UC mice. Furthermore, LBP 2–1 alleviated UC symptoms by enhancing microbiota diversity, increasing the abundance of beneficial bacteria (e.g., norank_f__Muribaculaceae ), and reducing harmful bacteria (e.g., Bacteroides ). These findings elucidate the structural characteristics of a novel acidic polysaccharide and confirm its therapeutic potential for UC treatment.
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JOURNAL OF SEPARATION SCIENCE
·
2025
·Vol. 48
·期 9
·e70265
·
IF 2.8
Yingying Zhang,Ruixue Zhang,Xuzhao Yang,Longhuan Wu,Ying Li,Yakun Li,Dan Ping,Runyi Liu,Daming Wu,Xintong Jiang
The extraction of bioactive compounds from natural plants has received considerable attention, with deep eutectic solvents (DESs) being considered as a novel kind of green solvent in the extraction processes. To extract bioactive ingredients from natural plants, a series of DESs was synthesized by combining a newly Gemini ionic liquid (IL), C 3 (MIM) 2 Br 2 with ethylene glycol (EG) in various molar ratios (1:3, 1:4, 1:5, and 1:6). The structure of the C 3 (MIM) 2 Br 2 /EG(1:6) system was characterized using Fourier-transform infrared spectroscopy, as well as 1 H and 13 C nuclear magnetic resonance spectroscopy. The synthesized DESs were employed for the ultrasonic-assisted extraction (UAE) of flavonoids from Lycium barbarum L., and the flavonoid was quantified using ultraviolet-visible spectroscopy. Key process parameters, including extraction time, temperature, and solid-liquid ratio, were optimized through single-factor analysis and L 9 (3 3 ) orthogonal array experiments. The results demonstrated optimal extraction conditions as follows: extraction time of 40 min, extraction temperature of 50°C, and a solid-liquid ratio of 1/50 (g/mL) (the ratio of the mass of the solid material to the volume of the liquid as the extract, g/mL). Statistical analysis revealed that extraction temperature and extraction time had both probability values less than 0.05, indicating a significant impact on the extraction rate, and the extraction yield of flavonoids reached 17.94 mg/g, exceeding that achieved with 60% ethanol. The Gemini IL-based DESs exhibited distinct advantages in flavonoid extraction compared to traditional organic solvents. Additionally, the mechanisms underlying UAE using DESs were investigated through thermogravimetric analysis and scanning electron microscopy. The findings indicate that ultrasonically-assisted incorporation of Gemini ILs holds promise for advancing the extraction of bioactive substances.
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