SENSORS AND ACTUATORS B-CHEMICAL
·
2026
·
IF 8.3
Di Wu,Wei Chen,Chi Zhang,Xiaoxia Yao,Weixuan Shao,Yuxin Zhang,Zirui Mo,Zhengchun Liu
Continuous, accurate, and stable detection of the steroid hormone cortisol is essential for evaluating and managing human physiological and psychological states. This study presents a wearable stress monitoring device (WSMD) that integrates gold nanoparticle (AuNP) modified Laser-Induced Graphene (LIG) with a custom low-power electronic system for wireless real-time cortisol monitoring. Precise control of gold nanoparticle (AuNP) growth on LIG via electrochemical deposition facilitated adhesion of the electropolymerized polypyrrole (PPy) MIP layer onto the electrode surface and enhanced embedment of Prussian blue (PB) as a built-in redox probe. This process endowed the fabricated flexible sensor with triple enhancement in electrochemical active surface area (ECSA), surface roughness, and redox activity, enabling a broad cortisol detection range of 1 pM to 100 µM (R² = 0.996) with high sensitivity (2790 nA/lg(C(nM))), reproducibility, and regenerability. ELISA validation confirms that WSMD enables real-time detection of cortisol concentrations in sweat samples in vitro, offering a feasible solution for daily stress monitoring.
✓ 已验证
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2025
·
IF 6
Yafei Wang,Haitao Wang,Yan Zhang,Wei Li,Sijia Gao,Jiyan Qiao,Lei Zou,Zhuo Zhao,Ying Guan,Yongjun Zhang
Background Ultra-sensitive detection of biomarkers is critical for disease diagnosis and other applications. Immunoassays, particularly enzyme-linked immunosorbent assay (ELISA), are commonly used for protein biomarker detection but often suffer from insufficient sensitivity at low concentrations. Enhancing the detection capacity of ELISA for early diagnosis is therefore essential. Results Herein, a simple and cost-effective strategy was developed to enhance ELISA performance by pre-enriching target proteins using molecularly imprinted polymers (MIPs). MIPs, synthesized with high absorption capacity and specificity, were prepared using TrF (Transferrin) as the template and polypeptide as crosslinker. Target protein was pre-enriched through large-volume adsorption followed by small-volume elution. This pre-enrichment process resulted in an 8.25-fold increase in protein concentration, significantly improving ELISA sensitivity and reducing the limit of detection (LOD) by nearly an order of magnitude. The enhanced detection performance was validated in terms of accuracy, precision, and linearity. Significance This method, which combines MIP-based protein enrichment with ELISA detection, offers a promising and cost-effective solution for enhancing ELISA performance. It holds great potential as a widely applicable strategy for the sensitive detection of biomarkers in disease diagnostics.
研究领域:
生物化学
·
医学诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Analytical Methods
·
2019
·Vol. 13
·期 2
·403-411
·
IF 2.413
Liu Weihua,Wang Jing,Yu Wenlong,Wang Xianghong
Flumequine (FLU) is widely used as an antibiotic in the treatment and control of animal diseases, which may result in the presence of residues harmful to humans in food products of animal origin. A novel and fast biomimetic enzyme-linked immunosorbent assay (BELISA) was developed for the determination of FLU based on a molecularly imprinted polymer (MIP) as a selective affinity agent. With an optimized molar ratio of FLU to methacrylic acid (MAA) of 1:2 determined by molecular dynamic simulations, the MIP was directly synthesized on the surface of a 96-well plate (as solid support) with FLU as the template molecule, MAA as the functional monomer, ethylene glycol dimethacrylate (EGDMA) as the cross-linker, and azodiisobutyronitrile (AIBN) as the initiator. Under the optimized conditions, the BELISA method was established with a sensitivity (IC 50 ) of 0.141 μg/mL and a detection limit (IC 15 ) of 1.09 × 10 −3 μg/mL. The developed method was applied to determine FLU concentrations in spiked beef and shrimp samples, with satisfactory recovery values ranging from 80.7 to 92.2% with relative standard deviations ( n = 3) between 3.3% and 7.0%. This method can be used for the rapid determination of FLU in animal foods and has promising applications in food safety monitoring.
研究领域:
分析化学
·
食品安全
·
生物技术
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2019
·Vol. 30
·期 24
·21177-21184
·
IF 2.195
Zheng Jiahong,Cheng Kangkang,Yu Pengfei
A simple fluorescence sensor for the detection of sulfadimidine (SM 2 ) was fabricated by molecular imprinting technology. Cadmium telluride quantum dots (CdTe QDs) as the fluorescent material, 3-aminopropyltriethoxysilane (APTES) as functional monomer, sulfadimidine as template molecule and tetraethoxysilane (TEOS) as cross-linker, the CdTe QDs were embedded in silica nano-particles by reverse microemulsion method. Under optimal detection condition, response is linear in the 2–30 μmol L −1 SM 2 concentration condition with the detection limit was 0.4 μmol L −1 . In addition, the correlation coefficient was 0.9857 and the imprinting factor was 4.82. The fluorescent probe was successfully applied to the determination of SM 2 in tap water and milk sample with the spiked recoveries ranging from 99.8 to 103.2%. It gets a good specific recognition ability and potential practical application value.
研究领域:
分析化学
·
材料科学
·
环境科学
✓ 已验证
DOI
高影响因子
LUMINESCENCE
·
2024
·Vol. 39
·期 8
·e4871
·
IF 3.2
Yaru Wang,Chaohai Pang,Xionghui Ma,Mingyue Wang,Xiaopeng Wu,Sixin Liu,Shuhuai Li
Graphene oxide (GO) and copper nanoparticles (Cu NPs) were incorporated to modulate and enhance the fluorescence properties of pegylated graphite phase carbon nitride (g-C 3 N 4 -PEG). Combined with the specific recognition capability of a molecular imprinted polymer (MIP), a highly sensitive and selective fluorescent molecular imprinted probe for dopamine detection was developed. The fluorescent g-C 3 N 4 -PEG was synthesized from melamine and modified with GO and Cu NPs to obtain GO/g-C 3 N 4 -PEG@Cu NPs. Subsequently, MIP was prepared on the surface of GO/g-C 3 N 4 -PEG@Cu NPs using dopamine as the template molecule. Upon elution of the template molecule, a dopamine-specific GO/g-C 3 N 4 -PEG@Cu NPs/MIP fluorescence probe was obtained. The fluorescence intensity of the probe was quenched through the adsorption of different concentrations of dopamine by the MIP, thus establishing a novel method for the detection of dopamine. The linear range of dopamine detection was from 5 × 10 −11 to 6 × 10 −8 mol L −1 , with a detection limit of 2.32 × 10 −11 mol L −1 . The sensor was utilised for the detection of dopamine in bananas, achieving a spiked recovery rate between 90.3% and 101.3%. These results demonstrate that the fluorescence molecular imprinted sensor developed in this study offers a highly sensitive approach for dopamine detection in bananas.
研究领域:
分析化学
·
生物传感
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 4
·1-10
·
IF 5.3
Cao Hui,Han Yiyi,Chen Zixin,Ding Xiner,Ye Tai,Yuan Min,Yu Jinsong,Wu Xiuxiu,Hao Liling,Yin Fengqin,Xu Fei
A novel molecularly imprinted nanomaterial (Eu (BTC)-MPS@MIP) was synthesized on the surface of silanized europium-based metal–organic frameworks (Eu (BTC)-MPS) using 1, 3, 5-benzotrioic acid (H 3 BTC) as a ligand. The resulting Eu (BTC)-MPS@MIP was applied to constructing a smartphone sensing platform for the sensitive and selective detection of clothianidin (CLT) in vegetables. The synthesized Eu (BTC)-MPS@MIP demonstrated the successful formation of a typical core–shell structure featuring a shell thickness of approximately 70 − 80 nm. The developed sensing platform based on Eu (BTC)-MPS@MIP exhibited sensitivity in CLT detection with a detection limit of 4 µg/L and a linear response in the range 0.01 − 10 mg/L at excitation and emission wavelengths of 365 nm and 617 nm, respectively. The fluorescence sensing platform displayed excellent specificity for CLT detection, as evidenced by a high imprinting factor of 3.1. This specificity is primarily attributed to the recognition sites in the molecularly imprinted polymer (MIP) layer. When applied to spiked vegetable samples, the recovery of CLT ranged from 78.9 to 102.0%, with relative standard deviation (RSD) values falling between 2.2 and 6.2%. The quenching mechanism of Eu (BTC)-MPS@MIP toward CLT can be attributed to the inner filter effect (IFE), resulting from the optimal spectral overlap between the absorption spectrum of CLT and the excitation spectra of Eu (BTC)-MPS@MIP. The proposed method has the potential for extension to the detection of other pesticides by replacing the MIP recognition probes. Graphical abstract
研究领域:
分析化学
·
食品安全检测
·
材料科学
✓ 已验证
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2019
·
IF 3.714
Jiadi Sun,Wei Guo,Jian Ji,Zaijun Li,Xiao Yuan,Fuwei Pi,Yinzhi Zhang,Xiulan Sun
As a kind of mycotoxins , patulin has been a usual contamination in apple juice and causes a serious public health concern nowadays. Regarding this issue, a novel and effective adsorbent, Fe 3 O 4 @SiO 2 @CS-GO@MIP, is designed to remove the patulin from apple juice. Firstly, the molecularly imprinted polymer (MIP) was prepared by surface imprinting technique. Meanwhile, addition of Fe 3 O 4 makes the final prepared MIP adsorbent magnetic, which is beneficial for separating the adsorbent from the food matrix. Moreover, chitosan (CS) and SiO 2 improve the biocompatibility, stability and dispersibility of the MIP adsorbent effectively. As for the adsorption performance, batches of experiments were conducted on the proposed adsorbent. Experimental results showed that the adsorption process followed pseudo-second-order kinetic and Freundlich isotherm model, and the physical multi-molecular layer endothermic adsorption happened during the whole adsorption process. Adsorption capacity of the proposed adsorbent is 7.11 mg/g maximumly, and over 90% of the total patulin in apple juice being removed after 24 h. Besides, the proposed adsorbent has distinguishing characteristics, including large specific surface area with BET reaching 279.6 m 2 /g, high cell viability of 79.77% and no negative effects on final quality parameters. The desirable adsorption performance and characteristics make Fe 3 O 4 @SiO 2 @CS-GO@MIP a promising adsorbent for removing the patulin from apple juice in the future.
研究领域:
食品安全
·
环境科学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
Food Analytical Methods
·
2016
·Vol. 10
·期 5
·1386-1396
·
IF 2.167
Wang Yu,Gao Lei,Qin Dongli,Chen Ligang
In this work, a simple and selective method based on matrix solid phase dispersion (MSPD) using molecularly imprinted polymers (MIPs) as sorbents was successfully developed for the determination of melamine (MEL) in milk powder. The MIPs have been prepared by surface imprinted polymerization method using carbon nanotube (CNT) as support, MEL as template molecule, γ-aminopropyltriethoxysilane as functional monomer, and tetraethyl orthosilicate as cross-linking agent. The MIPs were characterized by Fourier transform infrared spectrometer and scanning electron microscopy. At the same time, the adsorption isotherm, dynamic adsorption, and selective adsorption of MIPs to MEL were also studied. The prepared MIPs were used as dispersant of MSPD to extraction and separate MEL in milk powder. We optimized the extraction conditions as follows: the ratio of MIPs to sample was 2:3; the dispersion time was 10 min; washing solvent was 2.0 mL acetonitrile; and elution solvent was 4.0 mL ammonium methanol (5:95, v / v ). Once the MSPD process was completed, the MEL extracted from milk powder was determined by LC-MS/MS. Under the optimal conditions, the limit of detection of MEL was 0.2 ng g −1 . The relative standard deviations of intra-day and inter-day were obtained in the range of 0.4∼5.1 %. The satisfied recoveries of MEL were 92.5∼97.2 %. The MIP-MSPD method has some advantages, such as higher selectivity, lower cost of preparation, less time consuming and organic solvent consumption, simpler sample pretreatment procedure, and higher extraction recovery compared with those existing methods which were used for determination of MEL.
研究领域:
食品科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
DYES AND PIGMENTS
·
2018
·
IF 3.767
Ji Yang,Hao Wu,Ming-Hui Wu,Jun Zeng,Zheng-Zhong Lin,Xiao-Mei Chen,Zhi-Yong Huang
Malachite green (MG) is an industrial dye, but has been once abused in aquaculture for disease treatment. Because MG and its metabolite, leucomalachite green (LMG), present great harm to human health, it is essential to rapidly and accurately detect MG and LMG residues in aquatic products. However, the simultaneous screening of MG and LMG is still a challenge to date. In this work, a dual-template molecularly imprinted polymer (MIP) coated CdTe QDs was developed and applied as a nano-probe to simultaneously detect MG and LMG in fish samples via normal and synchronous fluorescence quenching. The molar ratios of templates (MG and LMG) to monomer (APTES) and cross-linker (TEOS), and the amount of CdTe QDs in the probe preparation were optimized. A normal fluorescence emission at 600 nm could be linearly quenched by MG, while the synchronous fluorescence emission at the wavelength interval of 340 nm could be quenched by both LMG and MG. The detection limits of MG and LMG were 17 nmol L −1 and 28 nmol L −1 (3 σ , n = 9), respectively. Based on the equations of Stern-Volmer, MG and LMG could be simultaneously detected without any conversion treatment. The recoveries of MG and LMG in fish samples ranged from 92% to 107% and from 91% to 108%, respectively. The results demonstrated that the as-prepared dual-template CdTe@MIP could be used as a fluorescent probe to rapidly and simultaneously detect MG and LMG in fish samples.
研究领域:
分析化学
·
食品安全
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
ELECTROANALYSIS
·
2016
·Vol. 28
·期 9
·2044-2049
·
IF 2.471
Juanjuan Zuo,Xiaoyu Zhao,Xiaocui Ju,Shue Qiu,Wenshuai Hu,Ting Fan,Juankun Zhang
A novel molecularly imprinted polymer (MIP) sensor for rapid determination of cardiac troponin (cTnI) was established. Since it can bind to the template molecule cTnI specifically, it can be used to detect concentration of cTnI in serum without much sample pretreatment. What's more, the electrochemical signals depend on the concentration of template molecules. The synthetic sensor possesses advantages including simplicity, high specificity, low cost of preparation, good chemical and mechanical properties, sensitive and label-free determination. The synthetic sensor shows good dynamic linearity at concentration range from 0.05 to 5.00 nM. The limit of detection (LOD) was found to be 0.027 nM. The detection time of whole process was within 5 minutes.
研究领域:
生物医学工程
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
Analytical Methods
·
2014
·Vol. 7
·期 4
·1387-1394
·
IF 1.938
Lu Lin,Hui-Ting Lian,Xiang-Ying Sun,Ya-Ming Yu,Bin Liu
A molecularly imprinted polymer (MIP) based sensor for detecting L-dopa was fabricated based on the composite of graphene (GR) and chitosan (CS). The microstructure and composition of the imprinted films characterized by scanning electron microscopy (SEM), Fourier transform infrared (FTIR), Raman spectroscopy and electrochemical impedance spectroscopy (EIS) indicated that the modified film of GR-MIPs had been formed successfully. Electrochemical evaluation results showed that the sensor exhibited a satisfactory selectivity for molecular binding with an imprinting factor (IF) of 6.2 and a wide linear range from 0.4 μM to 100 μM with a low detection limit of 0.012 μM. This electrochemical sensor exhibited great promise for accurate determination of L-dopa in pharmaceutical preparations and human blood serum for recoveries ranging from 95.0 to 108.0%. Furthermore, compared to imprinted films without graphene doping, GR-MIPs showed a superior chiral sensing for L-dopa in the presence of D-dopa. The results reveal that the insertion of graphene brought about an enhanced sensitivity for chiral sensing and has the potential for application in chiral recognition.
研究领域:
分析化学
·
材料科学
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
INORGANIC CHEMISTRY COMMUNICATIONS
·
2026
·
IF 5.9
Yi Li,Jing Zhang,Jing Shao
Antibiotics are widely used in the treatment of various diseases in humans and animals, and the detection of metabolic residual antibiotics has become an important research task. Flexible surface-enhanced Raman scattering (SERS) substrates are in great demand for sensitivity and specificity detection of antibiotics. Herein, we constructed a surface-enhanced Raman scattering-molecular imprinting polymer sensor (SERS-MIP) consisting of polyvinyl chloride (PVC) membrane loaded with silver nanoparticles and molecular imprinting layer. The surface of PVC was aminated by nucleophilic substitution reaction as a carrier for the formation of silver nanoparticle. The molecular imprinting method of atom transfer-electron transfer radical polymerization was used to realize the controlled growth of polymer for constructing SERS-MIP sensor, and its detection capability for ciprofloxacin reached 10 −9 M. Simultaneously, the stability, uniformity, reusability, and selectivity of the sensor all showed good performance. The characteristic peaks of ciprofloxacin were still visible in the mixed solution with other interferents. The results indicated that SERS-MIP sensor could realize sensitivity and specificity detection in complex environment.
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）