CHEMICAL ENGINEERING JOURNAL
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2024
·
IF 13.3
Fuchen Jiang,Qing Li,Yingxi Li,Xiaomin Lai,Yun Duan,Arlene McDowell,Zhen Huang,Shuang Liu,Ying Wang,Chen Zhang,Yan Qu,Xiaoli Pan
In biomedicine, one of the major challenges is effectively promoting wound healing of bacterial infections. Current wound dressings (e.g., gauze and bandages) are far from meeting the needs of the population as they lack various bioactivities to promote wound healing (including hemostasis, antimicrobial, anti-inflammatory, promotion of tissue regeneration, etc.) and are not biodegradable. In this study, we prepared an insect chitosan/pullulan/citric acid three-dimensional, layered electrospun nanofiber sponge embedded with ZnMOF in situ (3D-ZnMOF) and used it to promote MRSA-infected wound healing. The fabrication method and physicochemical characteristics of 2D nanofiber membranes and 3D nanofiber sponges were thoroughly assessed. SEM-EDS, XPS, XRD, and FT-IR were used to characterize the 3D-ZnMOF sponges, and the release behavior of zinc ions was examined. Experiments conducted both in vitro and in vivo demonstrated the hemostatic characteristics, biocompatibility, and antibacterial activity of 3D-ZnMOF sponges. 3D-ZnMOF sponges showed the ability to promote wound contraction, accelerate collagen deposition, inhibit inflammatory factors, and promote angiogenesis in an MRSA-infected rat whole-skin wound model. Consequently, 3D-ZnMOF nanofiber sponges offer a great deal of promise for accelerating the healing of wounds infected with drug-resistant bacteria.
研究领域:
生物医学工程
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材料科学
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微生物学
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DOI
高影响因子
Advanced Healthcare Materials
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2023
·
IF 10
Kai Lv,Guowei Li,Xiangjun Pan,Luxuan Liu,Ziheng Chen,Yu Zhang,Hao Xu,Dong Ma
Currently, undeveloped diagnosis and delayed treatment of bacteria-infected sites in vivo not only expand the risk of tissue infection but are also a major clinical cause of multiple drug-resistant bacterial infections. Here, an efficient nanoplatform for near-infrared (NIR)-light-controlled release and bacteria-targeted delivery of nitric oxide (NO) combined with photothermal therapy (PTT) is presented. Using maltotriose-decorated mesoporous polydopamine (MPDA-Mal) and BNN6, a smart antibacterial (B@MPDA-Mal) is developed to combine bacterial targeting, gas-controlled release, and PTT. Utilizing bacteria's unique maltodextrin transport system, B@MPDA-Mal can accurately distinguish bacterial infection from sterile inflammation and target the bacteria-infected sites for efficient drug enrichment. Moreover, NIR-light causes MPDA to generate heat, which not only effectively induces BNN6 to produce NO, but also raises the temperature to harm the bacteria further. NO/photothermal combination therapy effectively eliminates biofilm and drug-resistant bacteria. The myositis model of methicillin-resistant Staphylococcus aureus infection is established and indicates that B@MPDA-Mal can successfully eradicate inflammation and abscesses in mice. Meanwhile, magnetic resonance imaging technology is used to monitor the treatment procedure and healing outcomes. Given the aforementioned advantages, the smart antibacterial nanoplatform B@MPDA-Mal can be used as a potential therapeutic tool in the biomedical field against drug-resistant bacterial infections.
研究领域:
生物医学工程
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药学
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传染病学
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纳米技术
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治疗诊断学
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产品关联
DOI
PubMed
高影响因子
Advanced Science
·
2024
·
IF 14.3
Qian Liu,Xueliang Liu,Xiaojun He,Danyan Wang,Chen Zheng,Lin Jin,Jianliang Shen
Patients with methicillin-resistant Staphylococcus aureus (MRSA) infections may have higher death rates than those with non-drug-resistant infections. Nanozymes offer a promising approach to eliminating bacteria by producing reactive oxygen species. However, most of the conventional nanozyme technologies encounter significant challenges with respect to size, composition, and a naturally low number of active sites. The present study synthesizes a iron-single-atom structure (Fe-SAC) via nitrogen doped-carbon, a Fe-N 5 catalyst (Fe-SAC) with a high metal loading (4.3 wt.%). This catalyst permits the development of nanozymes consisting of single-atom structures with active sites resembling enzymes, embedded within nanomaterials. Fe-SAC displays peroxidase-like activities upon exposure to H 2 O 2 . This structure facilitates the production of hydroxyl radicals, well-known for their strong bactericidal effects. Furthermore, the photothermal properties augment the bactericidal efficacy of Fe-SAC. The findings reveal that Fe-SAC disrupts the bacterial cell membranes and the biofilms, contributing to their antibacterial effects. The bactericidal properties of Fe-SAC are harnessed, which eradicates the MRSA infections in wounds and improves wound healing. Taken together, these findings suggest that single Fe atom nanozymes offer a novel perspective on the catalytic mechanism and design, holding immense potential as next-generation nanozymes.
研究领域:
纳米技术
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催化
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生物医学工程
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材料科学
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抗菌研究
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF TOXICOLOGICAL SCIENCES
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2019
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IF 1.732
Nana Long,Huaqiao Tang,Lin Lin,Jianlong Li,Lijuan Guo,Fenghui Sun,Min Dai
Methicillin-resistant Staphylococcus aureus (MRSA) leads to serious infections, but it is not known whether it changes the expression of kidney drug metabolizing enzymes during infection. The mice were infected with different doses of MRSA and the oxidative stress and inflammation levels in the kidney were examined. The mRNA expression and activity of cytochrome P450 enzyme was analysed. Mice infected with high levels of MRSA showed a decrease in renal antioxidant capability and an elevated level of oxidative metabolites, which was accompanied by the release of inflammatory cytokines. The levels of interleukin 1β, tumour necrosis factor alpha, and macrophage inflammatory protein-1α were significantly increased along with the levels of nitric oxide and malondialdehyde. On day 7, mRNA expression of Cyp1a2, 2d22, and 3a11 were decreased by the high level of MRSA, but the low level of MRSA increased their expressions. Cyp2e1 mRNA expression was increased by MRSA in the kidney of mice. High dose of MRSA infection increased the oxidative stress and inflammatory response in mouse kidney, leading to the decrease in the expression of renal drug-metabolizing enzymes and no recovery within 7 days.
研究领域:
药理学
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微生物学
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病理学
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产品关联
DOI
PubMed
高影响因子
Foods
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2025
·Vol. 14
·期 18
·3236
·
IF 5.1
Xiaoyong Huang,Huiting Yang,Chenli Wang,Shiqi Yan,Xiaomin Ren,Zilong Sun
The relentless spread of antimicrobial resistance poses a severe threat to global public health, food safety, and environmental security. Natural products with potent antimicrobial activity offer prospective substitutes for traditional antibiotics and chemical preservatives. Here, we demonstrate that pterostilbene (PT), a natural dietary compound, exhibits rapid lytic activity against methicillin-resistantStaphylococcus aureus(MRSA). PT displayed metabolism-independent bactericidal effects, effectively eradicating dormant persister cells within one hour, though its activity was partially attenuated under anaerobic conditions. Mechanistically, PT disrupted membrane integrity by increasing permeability, dissipating membrane potential, depleting cellular ATP, and suppressing reactive oxygen species (ROS) generation. Its efficacy was modulated by membrane phospholipid composition, with phosphatidylglycerol (PG) and cardiolipin (CL) critically influencing antimicrobial potency. Crucially, PT robustly inhibited MRSA biofilm formation in milk. These findings highlight PT’s potential as a structurally stable, natural antimicrobial for controlling resilient MRSA, particularly against biofilm-associated and persister subpopulations in food systems.
研究领域:
微生物学
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抗菌研究
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食品安全
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天然产物
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生物化学
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产品关联
DOI
PubMed
高影响因子
Frontiers in Immunology
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2025
·
IF 5.9
Lingdi Niu,Haoyuan Duan,Jiaqing Wang,Zheng Jia,Hai Li,Junjie Guo,Shuhe Zhang,Ning Liu,Yaxin Miao,Junwei Ge,Fang Wang
Trained immunity refers to the ability of trained innate immune cells to generate an immune memory that produces rapid, broad-spectrum, and long-lasting protection against heterologous stimuli. Based on the rapid and broad-spectrum protection that the peptidoglycan backbone from lactic acid bacteria, bacterium-like particles (BLPs), offers, we hypothesized that BLPs enhance protection through trained immunity. Here, we found that combining BLP with a vaccine significantly improves protective efficacy against methicillin-resistant Staphylococcus aureus (MRSA) infection, accompanied by changes in trained immunity markers. We demonstrate that BLP-induced trained immunity macrophages exhibit increased cytokine secretion and phagocytic activity in vitro. In an in vivo model, BLP confers protection against S. aureus 26003 even without specific antigens. In an ex vivo model, BLP induces increased markers of trained immunity. Transcriptome analysis suggests that BLP may induce trained immunity by activating the IL-6-JAK-STAT3 pathway through TLR2 receptor activation, thereby modulating macrophage metabolic reprogramming and function. In summary, our study establishes that BLP induction of trained immunity, along with regulated metabolic reprogramming and macrophage function, may contribute to enhancing vaccine efficacy. Our findings elucidate a novel mechanism for BLP-mediated immune enhancement, critical for the application of BLP as a vaccine vector to construct a vaccine that combines specific immune response with innate immune response.
研究领域:
免疫学
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微生物学
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疫苗学
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分子生物学
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传染病学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF MEDICINAL CHEMISTRY
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2025
·
IF 6.8
Lei Xu,Yaling Wang,Sangyu Hu,Yuzhu Pei,Chenliang Qian,Wenjie Xue,Gao Zhang,Song Wu,Xinxin Si,Xuming Deng,Jie Xia,Jianfeng Wang
To date, the abuse of antibiotics and a gradual decline in novel antibiotic discovery enlarge the threat of drug-resistant bacterial infections, especially methicillin-resistant Staphylococcus aureus (MRSA). Herein, inspired by the unique structures and antibacterial activities of 2-quinolones, a class of novel 2-quinolones with substituted pyridines was synthesized. Notably, compound 11, the derivative with a methylpyridine fragment, showed potent antibacterial and antibiofilm activities, especially for MRSA strains (MIC = 0.02–0.04 μg/mL). A mechanistic study of compound 11 revealed that the increase of intracellular ROS and acceleration of the TCA cycle, which reprogrammed the bacterial metabolism, eventually triggered membrane damage and bacterial death. Most importantly, compound 11, with antibacterial and anti-inflammatory properties, accelerated the reconstruction and healing of MRSA-infected cutaneous wounds by decreasing bacterial loads, attenuating inflammation, and promoting angiogenesis. Overall, these findings provide a novel multifunctional chemotype with broad-spectrum antibacterial activity and highlight a promising strategy for MRSA-infected wound healing.
研究领域:
药理学
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微生物学
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化学
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传染病学
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伤口愈合
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
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2013
·Vol. 14
·期 1
·1802-1821
·
IF 2.464
Rongxin Qin,Kangkang Xiao,Bin Li,Weiwei Jiang,Wei Peng,Jiang Zheng,Hong Zhou
Fructus crataegi (hawthorn) is the common name of all plant species in the genusCrataegusof the Rosaceae family. In the present study, three monomers of (+)-catechin (C), (−)-epicatechin gallate (ECg) and (−)-epigallocatechin (EGC) were isolated from the hawthorn under the guide of antibacterial sensitization activity. The bioactivity of the composite fraction in enhancing the antibacterial effect of oxacillin against methicillin-resistantStaphylococcus aureus(MRSA) was greater than that of the individual monomer of the hawthorn extractin vitro. Two-fold dilution and checkerboard methods were used to analyze antibacterial activity and screen for the combination and proportion of monomers with the best bioactivity. The result showed that C (128 mg/L) combined with ECg (16 mg/L) had the greatest effect and the combination also reduced the bacterial load in blood of septic mice challenged with a sublethal dose of MRSA, increased daunomycin accumulation within MRSA and down-regulated the mRNA expression ofnorA,norCandabcA, three important efflux pumps of MRSA. In summary, C and ECg enhanced the antibacterial effect of β-lactam antibiotics against MRSAin vitroandin vivo, which might be related to the increased accumulation of antibiotics within MRSA via suppression of important efflux pumps’ gene expression.Keywords:fructus crataegi;(+)-catechin;(−)-epicatechin gallate;synergistic effect;MRSA;drug accumulation
研究领域:
药理学
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微生物学
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植物化学
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传染病学
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产品关联
DOI
PubMed
高影响因子
BIOMACROMOLECULES
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2023
·
IF 6.2
Zhiwen Zeng,Jiayi Guo,Guangxin Shen,Cuiping Guo,Dating Pei,Daohuan Lu,Zhijie Geng,Jun Huang,Shan Yu
Chronic wound infection often leads to irregular tissue closure and accompanies delayed healing and economy issues. Developing an ideal wound dressing that can control the occurrence of antibacterial infections and biological responses is highly desirable. In this study, a multifunctional hybrid hydrogel (GS@EG–Cu–CA NPs) containing synthesized thiolated gelatin, methacrylated silk fibroin, and (−)-epigallocatechin gallate–copper ionic–carrageenan nanoparticles (EG–Cu–CA NPs) was engineered by a thio–ene click reaction. The metal–polyphenol EG–Cu–CA NPs were encapsulated with kappa-carrageenan to enhance its aqueous-soluble, mechanical, and bioactive properties and endowed the hydrogel dressing with fascinating antibacterial, antioxidation, and nitric oxide (NO) generation by catalyzing. The hybrid hydrogels also illustrated a favorable cytocompatibility. Benefiting from the thio–ene click reaction, the hybrid hydrogels were injected and photocured rapidly in situ to cover an irregular wound. In an SD rat full-thickness skin-wound-infected model, the methicillin-resistant Staphylococcus aureus-infected wound covered with GS@EG–Cu–CA NPs was almost completely healed after 10 days. This study presents a facile design of hydrogel dressing incorporating metal–polyphenol nanoparticles, which demonstrates a promising potential way for dealing with effective wound infection management and other complicated wound healings.
研究领域:
生物材料
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伤口愈合
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纳米技术
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
PHYTOMEDICINE
·
2022
·
IF 6.656
Zhonghao Li,Qinmei Sun,Qingyang Liu,Xinru Mu,Hui Wang,Han Zhang,Fenfen Qin,Qisheng Wang,Dengyun Nie,Anlong Liu,Qian Li,Jianjian Ji,Yongwei Jiang,Shengfeng Lu,Qian Wang,Zhigang Lu
Background Opioids are widely used in clinical practice. However, their long-term administration causes respiratory depression , addiction, tolerance, and severe immunosuppression . Traditional Chinese medicine (TCM) can alleviate opioid-induced adverse effects. Compound 511 is particularly developed for treating opioid addiction, based on Jiumi Liangfang, an ancient Chinese drug treatment and rehabilitation monograph completed in 1833 A.D. It is an herbal formula containing eight plants, each of them contributing to the overall pharmacological effect of the product: Panax ginseng C. A. Meyer (8.8%), Astragalus membranaceus (Fisch.) (18.2%), Datura metel Linn. (10.95%), Corydalis yanhusuo W. T. Wang (14.6%), Acanthopanar gracilistμlus W. W. Smith (10.95%), Ophiopogon japonicus (Linn. f.) Ker-Gawl. (10.95%), Gynostemma pentaphyllum (Thunb.) Makino (10.95%), Polygala arvensis Willd. (14.6%). This formula effectively ameliorates opioid-induced immunosuppression. However, the underlying mechanism remains unclear. Purpose To reveal the effects of Compound 511 on the immune response of morphine-induced immunosuppressive mice and their potential underlying molecular mechanism. This study provides information for a better clinical approach and scientific use of opioids. Methods Immunosuppression was induced in mice by repeated morphine administration. Th1/Th2/Th17/Treg cell levels were measured using flow cytometry. Splenic transcription factors of Th1/Th2/Th17/Treg and outputs of the regulatory PI3K/AKT/mTOR signaling pathway were determined. Subsequently, methicillin-resistant Staphylococcus aureus (MRSA) was administered intranasally to morphine-induced immunosuppressive mice pretreated with Compound 511. Their lung inflammatory status was assessed using micro-computer tomography (CT), hematoxylin and eosin (H&E) staining, and enzyme-linked immunosorbent assay (ELISA). Results Compared to morphine, Compound 511 significantly decreased the immune organ indexes of mice, corrected the Th1/Th2 and Treg/Th17 imbalance in the immune organs and peripheral blood, reduced the mRNA levels of FOXP3 and GATA3 , and increased those of STAT3 and T-bet in the spleen . It improved immune function and reduced MRSA-induced lung inflammation. Conclusion Compound 511 ameliorates opioid-induced immunosuppression by regulating the balance of Th1/Th2 and Th17/Treg via PI3K/AKT/mTOR signaling pathway. Thus, it effectively reduces susceptibility of morphine-induced immunosuppressive mice to MRSA infection .
研究领域:
药理学
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免疫学
·
微生物学
·
中药学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
Theranostics
·
2020
·
IF 8.579
Chaoli Wang,Peng Chen,Youbei Qiao,Yuan Kang,Chaoren Yan,Zhe Yu,Jian Wang,Xin He,Hong Wu
Background : Biofilm infection caused by multidrug-resistant bacteria is difficult to eradicate by conventional therapies. Photodynamic therapy (PDT) is an effective antibacterial method for fighting against biofilm infection. However, the blocked photosensitizers outside of biofilm greatly limit the efficacy of PDT. Methods : Herein, a novel acid-responsive superporogen and photosensitizer (SiO 2 -P Ce6-IL ) was developed. Because of the protonation of the photosensitizer and the high binding energy of the polyionic liquid, SiO 2 -P Ce6-IL changed to positive SiO 2 -P IL + in an acidic microenvironment of biofilm infection. SiO 2 -P IL + could combine with negatively charged extracellular polymeric substances (EPS) and create holes to remove the biofilm barrier. To strengthen the interaction between SiO 2 -P IL + and EPS, SiO 2 -P IL + of high charge density was prepared by grafting the high-density initiation site of ATRP onto the surface of the SiO 2 base. Results : Due to the rapid protonation rate of COO - and the strong binding energy of SiO 2 -P IL + with EPS, SiO 2 -P Ce6-IL could release 90% of Ce6 in 10 s. With the stronger electrostatic and hydrophobic interaction of SiO 2 -P IL + with EPS, the surface potential, hydrophobicity, adhesion and mechanical strength of biofilm were changed, and holes in the biofilm were created in 10 min. Combining with the release of photosensitizers and the porous structure of the biofilm, Ce6 was efficiently concentrated in the biofilm. The in vitro and in vivo antibacterial experiments proved that SiO 2 -P Ce6-IL dramatically improved the PDT efficacy against MRSA biofilm infection. Conclusion : These findings suggest that SiO 2 -P Ce6-IL could rapidly increase the concentration of photosensitizer in biofilm and it is an effective therapy for combating biofilm infection.
研究领域:
材料科学
·
纳米技术
·
生物医学工程
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微生物学
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药学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Jian Yang,Dongyu Wang,Haojie Yu,Li Wang,Yun Wang,Xiaowei Liu,Yudi Huang,Chenguang Ouyang,Yichuan Hong,Shuning Ren,Yu Wang,Yang Jin,Jian Hu,Jingyi Feng
The infected diabetic wound healing is an increasingly severe healthcare problem worldwide. Bacterial infection and the inflammatory microenvironment hinder diabetic wound healing. Meanwhile, the combination of inhibiting bacterial growth and promoting macrophage polarization in the wound microenvironment is beneficial for treating diabetic wounds. Nowadays, hydrogels, as an emerging wound dressing, have great potential to replace or supplement traditional bandages or gauze. Here, glycyl methacrylate gelatin (Gel-Gym), oxidized hyaluronic acid (HA-CHO) and lauric acid (LA) were used to prepare the composite hydrogel (GH/LA) in addressing the clinical dilemma. The hydrogel could withstand 50 % compression deformation, its swelling rate was as low as 18 %, and its adhesion to pig skin reached 14 kPa. Moreover, a diabetic infected wound model was used to evaluate the feasibility of GH/LA hydrogel in vivo . The hydrogels' antimicrobial, anti-inflammatory and prorestitutive potentials were further investigated, and GH/LA showed a therapeutic effect on diabetic wounds. Interestingly, macrophage polarization into the M2 phenotype was significantly enhanced in the presence of GH/LA via GPR40/NF-κB pathway. This study provided a new avenue for treating methicillin-resistant staphylococcus aureus ( MRSA ) infected diabetic wounds.
研究领域:
生物医学工程
·
材料科学
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药剂学
·
伤口愈合
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感染性疾病
·
免疫学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）