Acta Pharmaceutica Sinica B
·
2026
·
IF 14.6
Luyao Yu,Yingxia Guo,Tonghe Liu,Yunlong Sheng,Meichen Liu,Ning Zhang,Jiye Tian,Chunpeng Feng,Meiyun Shi,Lei Yin
Paclitaxel (PTX) has poor aqueous solubility and vehicle-related toxicity. Polymeric micelles (PMs) can improve solubility and leverage the enhanced permeability and retention (EPR) effect for tumor targeting. However, a comprehensive understanding of their in vivo fate remains limited, due to the inability to distinguish between encapsulated and released drug. We developed a novel method integrating stable isotope tracing, ultrafiltration, and ultra-performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) to quantify free, released, and total PTX, the polymeric carriers methoxy-poly(ethylene glycol) (molecular weight ∼2,000 Da) (mPEG2K) and methoxy-poly(ethylene glycol)- block -poly(D,L-lactic acid) (mPEG block MW ∼2000 Da, PLA block MW ∼2,000 Da) (mPEG2K–PLA2K) in rats following administration of PTX injection and paclitaxel-loaded polymeric micelles (PTX-PMs). PTX-PMs significantly altered the pharmacokinetics, reducing the initial dose–normalized initial plasma concentration ( C 0 /Dose) of free PTX to 27.0% of that from the injection. Altered tissue distribution was observed, with high accumulation in reticuloendothelial system (RES) organs and more complete clearance by 48 h. Excretion studies revealed a substantially higher cumulative fecal excretion of PTX after PTX-PMs administration, while the polymer carrier was predominantly excreted renally as mPEG2K. These findings provide critical insights into the in vivo disposition of PTX nanoformulations, supporting the rational design of PMs-based delivery systems.
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高影响因子
Frontiers in Plant Science
·
2016
·
IF 4.495
Gaofeng Zhou,Sue Broughton,Xiao-Qi Zhang,Yanling Ma,Meixue Zhou,Chengdao Li
Genome-wide association studies (GWAS) based on linkage disequilibrium (LD) have been used to detect QTLs underlying complex traits in major crops. In this study, we collected 218 barley (Hordeum vulgare L.) lines including wild barley and cultivated barley from China, Canada, Australia, and Europe. A total of 408 polymorphic markers were used for population structure and LD analysis. GWAS for acid soil resistance were performed on the population using a general linkage model (GLM) and a mixed linkage model (MLM), respectively. A total of 22 QTLs (quantitative trait loci) were detected with the GLM and MLM analyses. Two QTLs, close to markers bPb-1959 (133.1 cM) and bPb-8013 (86.7 cM), localized on chromosome 1H and 4H respectively, were consistently detected in two different trials with both the GLM and MLM analyses. Furthermore, bPb-8013, the closest marker to the major Al3+ resistance gene HvAACT1 in barley, was identified to be QTL5. The QTLs could be used in marker-assisted selection to identify and pyramid different loci for improved acid soil resistance in barley.
研究领域:
植物遗传学
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作物科学
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基因组学
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非生物胁迫抗性
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DOI
高影响因子
Cell Reports Methods
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2026
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IF 5.8
Zhaohui Cao,Wenlong Zuo,Lanxiang Wang,Lei Dai
Spatial mapping of microbial communities at single-cell resolution is opening up dimensions to understand microbiome organization and function. However, current fluorescence in situ hybridization (FISH) methods for microbiomes are limited by multiplexity and scalability. Here, we present the sequential error-robust FISH spatial mapping platform (SEER-Map) for fully automated imaging of complex microbial communities. We show that an integrated platform of fluidics control and fluorescence microscopy can perform 40 rounds of sequential FISH. We apply SEER-Map to profile complex microbial communities colonized on plant roots and identify distinct spatial patterns and species co-occurrence at the micron-scale. Our work establishes SEER-Map as a high-throughput and scalable platform for high-resolution spatial profiling of microbiomes.
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DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Hao Lu,Xieming Xu,Zhongyuan Zhang,Xiong Zhang,Renyan Huang,Guiqing Feng,Jintao Xu,Shaofan Wu,Yi Zheng,Xin Huang,Min Chen,Shuaihua Wang
Nondestructive X-ray imaging techniques provide insights into the internal structure and deformation defects of electronic components. Abnormal heating is a common phenomenon that often occurs with electronic component failure. However, the significance of invisible thermal signals is often overlooked due to the limitations of conventional scintillator and X-ray imaging techniques. Rare-earth-doped fluoride scintillators, known for their high-energy irradiation response and thermally stimulated luminescence (TSL) properties, offer the potential to simultaneously utilize thermal signals and nondestructive X-ray imaging. Herein, we present a thermal mapping-assisted X-ray 3D tomographic application based on a trap-modulated LiLuF 4 :Tb,Zn (LLF:Tb,Zn) scintillator. By controlling the trap density in the energy range of 0.66 to 1.77 eV, LLF:Tb,Zn microcrystals achieve over 60 days of X-ray energy storage and a 7.7-fold increase in TSL. Additionally, the light yield is 143 % higher than that of the previous level, and the detection limit reaches 3.83 nGy s −1 . Furthermore, utilizing refractive-index matching between LLF:Tb,Zn microcrystals and polyacrylate, a high-loading, large-area flexible scintillation film is developed for thermal and high spatial resolution X-ray imaging of electronic chips. The film has an imaging spatial resolution of 35 LP mm −1 . Finally, we demonstrate the application of LLF:Tb,Zn films for thermal mapping-assisted 3D X-ray tomography reconstruction of an electronic component, offering a novel approach to multimodal X-ray imaging.
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高影响因子
Small
·
2025
·
IF 12.1
Guangxiang Gu,Xuejiao Wang,Jinyang Zhang,Zhong Lin Wang
In situ detection of contamination in liquid food production by intelligent sensing equipment in a factory is of great significance to ensure food safety, quality control, process optimization, and cost reduction. Current technologies require samples to be extracted and analyzed using bulky, expensive, and large instruments, which are time-consuming, complex, and cannot be realized in situ detection. In this work, a high-density array Triboelectric Nanogenerator (TENG) probe is fabricated by combining triboelectrification and charge transfer at liquid–solid interface, and interface charge transfer mapping (ICTM) is applied to the identification and safety detection of liquid foods. When a liquid food droplet slides across an insulated surface, the electrometer connected with the metal electrode array will record the electric signals, and the electric signals at each electrode will generate an ICTM. The ICTMs are sensitive to the compositions of various liquid food and additives, thus can be used to in situ detect contamination in liquid food production. Ten different liquid foods are successfully identified by ICTMs, as well as food additives and heavy metals in 5° white vinegar. The detection limit of Pb 2+ in 5° white vinegar is as low as 10 µg L −1 (in line with FDA standards), and the linear range is 0–10 µg L −1 . Finally, through the detection of 20 random samples, the detection accuracy of lead acetate in 5° white vinegar is 95%, and the detection accuracy of potassium sorbate is 90%. The array TENG probe and the generated ICTM open an approach for in situ and rapid food production and safety testing relying on cheap, portable, low-tech instrumentation.
研究领域:
食品科学
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纳米技术
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传感器
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分析化学
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电气工程
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DOI
PubMed
高影响因子
Nanomedicine-Nanotechnology Biology and Medicine
·
2021
·
IF 6.458
Jie Dong,Jinghua Sun,Wenwen Cai,Chunyan Guo,Qian Wang,Xuhui Zhao,Ruiping Zhang
Lymphatic metastasis plays an important role in malignant tumor invasion. Efficient identification of sentinel lymph node (SLN) is extremely significant for designing therapeutic strategies and assessing prognosis. In this work, we developed a natural cuttlefish melanin nanoprobe for the preoperative and intraoperative evaluation of lymphatic metastasis. The cuttlefish melanin nanoparticle could improve the water-solubility and biocompatibility of the near-infrared-II (NIR-II) dye, and extend the retention time of small molecule dye. The NIR-II imaging results verified that the nanoparticles have a high accumulation, high sensitivity, and high signal-to-noise ratio in the lymphatic system . Moreover, the nanoparticles have obvious naked-eye identification potential due to their natural brownish-black color. Additionally, the nanoparticles can combine with Gd ions to achieve preoperative lymphatic magnetic resonance imaging (MRI). The results of this study provide a unique approach to effectively identify and accurately remove lymph nodes before operation and during surgery, exhibiting tremendous potential in clinical translation.
研究领域:
医学影像
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生物医学工程
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肿瘤学
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材料科学
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PubMed
高影响因子
Frontiers in Chemistry
·
2020
·
IF 3.693
Hua Wei,Mao Yusheng,Zhang Jinzhu,Liu Lang,Zhang Guolin,Yang Shengyang,Boyer Daniel,Zhou Chen,Zheng Fenfen,Sun Shasha,Lin Shengling
Implantable optical sensing devices that can continuously monitor physiological temperature changes hold great potential toward applications in healthcare and medical field. Here, we present a conceptual foundation for the design of biocompatible temperature sensing device by integrating renal clearable luminescent gold nanoparticles (AuNPs) with silk film (AuNPs-SF). We found that the AuNPs display strong temperature dependence in both near-IR fluorescence intensity and lifetime over a large temperature range (10–60°C), with a fluorescence intensity sensitivity of 1.72%/°C and lifetime sensitivity of 0.09 μs/°C. When integrated, the AuNPs with biocompatible silk film are implanted in the dorsal region of mice. The fluorescence imaging of the AuNPs-SF in the body shows a linear relationship between the average fluorescence intensity and temperature. More importantly, <3.68% ID gold are left in the body, and no adverse effect is observed for 8 weeks. This AuNPs-SF can be potentially used as a flexible, biocompatible, and implantable sensing device for in vivo temperature mapping.
研究领域:
生物医学工程
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材料科学
·
纳米技术
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DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2019
·
IF 6.044
Chunxiang Ding,Chenyang Cai,Lixun Yin,Qinglin Wu,Mingzhu Pan,Changtong Mei
Strain-transfer plays a key role in overall modulus of mechanically adaptive nanomaterials. Herein, mechanically adaptive nanocomposites were prepared via introducing cellulose nanocrystal (CNC) percolating network into poly butyl methacrylate (PBMA) with polyethylene glycol (PEG) as a stabilizer. The prepared PBMA/CNC nanocomposites were soaked in deionized water at 23 °C and 37 °C for one week to investigate their mechanically adaption. The interactions between PEG, CNC , and PBMA were assessed by Fourier Transform infrared, X-ray diffraction, and X-ray photoelectron spectroscopy. Incorporation of PEG to CNC and PBMA/CNC nanocomposites on the morphological and thermal properties was also investigated. The mechanical adaption of PBMA/CNC nanocomposites after switching dry-to-wet state and surrounding temperature (soaked in deionized water at 23 °C and 37 °C for one week, respectively) was evaluated by mechanical testing. Meantime, digital image correlation (DIC) was firstly used to study strain transfer mechanism in mechanical adaption which was carried out in real-time synchronized with mechanical measurement. It indicated that PEG improved the dispersion of CNC in PBMA/CNC nanocomposite and its thermal properties. Furthermore, CNC with PEG modification bridged PBMA during crack propagation and promoted the stress and stain transfer in PBMA/CNC nanocomposites according to DIC analysis.
研究领域:
材料科学
·
纳米技术
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DOI
PubMed
高影响因子
NANOTECHNOLOGY
·
2021
·Vol. 32
·期 50
·505711
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IF 3.874
Shaojie Wang,Zhen Luo,Jiajie Liang,Simin Peng,Jun Hu,Jingliang He,Qi Li
Nanoscale mapping of electric polarizability in a heterogeneous dielectric material with surface irregularities is of scientific and technical significance, but remains challenging. Here, we present an approach based on intermodulation electrostatic force microscopy (EFM) in conjunction with finite element computation for precise and high-resolution mapping of polarizability in dielectric materials. Instead of using electrostatic force in conventional quantitative EFM approaches, the force gradient is acquired to achieve an unprecedented spatial resolution. In the meantime, the finite element model is applied to eliminate the interference from the heterogeneity and surface irregularity of the sample. This approach directly reveals the high polarization ability of the amorphous region in a ferroelectric, semi-crystalline polymer with significant surface roughness, i.e. poly (vinylidene fluoride-co-chlorotrifluoroethylene), in which the result is consistent with the predicted data in the latest research. This work presenting a quantitative approach to nanoscale mapping of electric polarizability with unprecedented spatial resolution may help to reveal the complex property-structure correlation in heterogeneous dielectric materials.
研究领域:
材料科学
·
物理学
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PubMed
高影响因子
Advanced Healthcare Materials
·
2023
·
IF 10
Dengjie Yu,Qingbin Nie,Jiangtao Xue,Ruizeng Luo,Shiwang Xie,Shengyu Chao,Engui Wang,Linlin Xu,Yizhu Shan,Zhuo Liu,Yusheng Li,Zhou Li
Osteoarthritis (OA) is the most prevalent joint degenerative disease characterized by chronic joint inflammation. The pathogenesis of OA has not been fully elucidated yet. Cartilage erosion is the most significant pathological feature in OA, which is considered as the result of the cytomechanical homeostasis destruction. The cytomechanical homeostasis is maintained by the dynamic interaction between cells and the extracellular matrix, which can be reflected by cell traction force (CTF). It is critical to assess the CTF for providing a deeper understanding of the cytomechanical homeostasis destruction and progression in OA. In this study, a silicon nanopillar array (Si-NP) with high spatial resolution and aspect ratio is fabricated to investigate the CTF in response to OA. We discover the CTF is degraded in OA, which is attributed to the F-actin reorganization induced by the activation of RhoA/ROCK signaling pathway. Si-NP also show promising potential as a mechanopharmacological assessment platform for OA drug screening and evaluation. This article is protected by copyright. All rights reserved
研究领域:
骨科
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生物力学
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纳米技术
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药理学
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DOI
PubMed
高影响因子
MATERIALS & DESIGN
·
2023
·
IF 8.4
Lu Chen,Bao Shi,Xinying Liu,Sainan Wei,Ge Fang,Ruosi Yan
Wearable flexible pressure sensors are a novel class of human activity detection device. However, the preparation of pressure sensors with high sensitivity and wide sensing range still faces great challenges. This study reveals an flexible heat-resistant variable density point pressure sensing array (PSA) with ultra-wide sensing range based on Ti 3 C 2 T x -MXene. MXene containing polar oxygen-containing functional groups coated polyester fiber fabricated the pressure sensing layer while a stainless steel wire core is used as a flexible electrode for signal collection. The signal processing device rapidly converts the mechanical signal into electrical signal output to increase the transmission speed and range. Experimental results show that the PSA can effectively sense dynamic and static pressures with high sensitivity (0.14–0.87 kPa −1 over a pressure range of 7.2 Pa–2000 kPa), a wide sensing range (0–15000 kPa), fast response time (80 ms), 10,000 cycles (2000 kPa) stability and maintains a 81.25% current response. The noval fully woven structural flexible PSA exhibited larger area, of which the weave density is varied to control resolution and the pressure mapping is referenced to each pixel point to qualitatively and quantitatively analyze shape and pressure distribution. Variable density points pressure sensor with wide sensing range and spatial pressure mappinghas promising applications in healthcare.
研究领域:
生物医学工程
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材料科学
·
传感器技术
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高影响因子
Photonics
·
2021
·Vol. 8
·期 5
·145
·
IF 2.676
Jianyi Xu,Xiaofeng Yin,Yisong Qi,Bo Chen,Yusha Li,Peng Wan,Yingtao Yao,Dan Zhu,Baoguo Jiang,Tingting Yu
The quantification and distribution characteristics of spinal motor neurons play important roles in the study of spinal cord and peripheral nerve injury and repair. In most research, the sole retrograde labeling of each nerve or muscle could not simultaneously obtain the distributions of different motor neuron subpopulations. Therefore, it did not allow mapping of spatial relationships of different motor neuron columns for disclosing the functional relationship of different nerve branches. Here, we combined the multiple retrograde labeling, optical clearing, and imaging for three-dimensional (3D) visualization of motor neurons of multiple brachial plexus branches. After screening fluorescent tracers by the labeling feasibility of motor neurons and fluorescence compatibility with optical clearing, we performed mapping and quantification of the motor neurons of ulnar, median, and radial nerves in the spinal cord, then disclosed the relative spatial distribution among different neuronal subpopulations. This work will provide valuable mapping data for the understanding of the functional relationships among brachial plexus branches, hopefully facilitating the study of regeneration of axons and remodeling of motor neurons in peripheral nerve repair.
研究领域:
神经生物学
·
再生医学
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DOI
高影响因子
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