BIOSENSORS & BIOELECTRONICS
·
2021
·
IF 10.618
Zhuoyue Zhou,Zhao Yang,Li Xia,Houjin Zhang
Chiral analysis of amino acids (AAs) is of great importance in medical science due to the distinctive effect of AA isomers on human health. Although various chiral recognition techniques have been developed, the quantitative chiral recognition of low-level AA isomers remains challenging. Here, we combined the fiber optic SPR with an enzyme-substrate recognition mechanism to construct a direct-assay-type chiral AA biosensor. As a proof-of-concept attempt, a recently discovered Rasamsonia emersonii D-amino acid oxidase (ReDAAO) with a wide substrate spectrum and high stability was immobilized on the graphene oxide and gold nanorods composites (GO-AuNRs), using both EDC/NHS coupling and the gold-binding peptide (GBP) method. Such a biosensor can distinguish two AA isomers at the same concentration. It achieved specific detection of D-amino acids (D-AAs) with a linear range from 5x10 -4 mM to 30 mM. Furthermore, it showed good resistance to enantiomeric interference. When the percentage of D-AA increases in the isomer mixture, a good linear relationship between the D/(D + L)-AA ratio and SPR spectral shift was obtained. This unique combination of the enzyme , nanocomposite , and SPR taps into the rich reservoir of proteins for chiral receptors. It lays the foundation for protein-based chiral recognition of other clinically important small molecules in future biosensor designs.
研究领域:
生物传感器
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分析化学
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生物医学工程
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纳米技术
·
酶学
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2022
·
IF 9.221
Min Chen,Yang Li,Rui Han,Qiuji Chen,Lingyun Jiang,Xiliang Luo
Herein, an electrochemical immunosensor capable of detecting targets in human serum with ultralow fouling and high sensitivity was successfully constructed through a click reaction. One designed biotinylated peptide was associated with the other peptide functionalized with biotin and azide groups at its two terminals, to form a branched antifouling peptide through the streptavidin-biotin affinity interaction, and it was attached to the electrode to construct an antifouling interface. Then, the exposed azide group on the branched peptide was used to link the antibody modified with 5′-dibenzocyclooctyne (DBCO), and the immunosensor was thus constructed with the aid of the click reaction between the azide group and the DBCO. The developed antifouling immunosensor demonstrated a wide linear response range for carcinoembryonic antigen (CEA) and a low limit of detection of 40 fg mL −1 , which is much superior to the sensing performance of the immunosensor prepared through the normal EDC/NHS method. The immunosensor was capable of assaying CEA in undiluted serum samples with satisfying accuracy when compared with a clinically approved method. This work offered an effective strategy to develop various biosensors through the conjugation of antifouling peptides with different antibodies.
研究领域:
分析化学
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生物化学
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生物传感器
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电化学
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癌症研究
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产品关联
DOI
高影响因子
Surfaces and Interfaces
·
2023
·
IF 6.2
Qianwen Yue,Cai Wang,Ke Zhang,Huiyu Wan,Baoliang Sun,Jingguo Wu,Jingyi Sun,Ying Wang
Thoracolumbar fascia (TLF) plays an important role in the biomechanics of the spine. TLF often causes low back pain (LBP), leading to limb movement disorders and numbness. At present, the clinical diagnosis mainly depends on doctors' clinical experience and magnetic resonance imaging. In order to make the diagnosis more convenient and accurate, we designed a "sandwich" biosensor based on surface-enhanced Raman scattering (SERS) to detect C-reactive protein (CRP) and serum amyloid A (SAA) in fascia. In this study, Ag nanocubes (AgNCs) were used as the substrate, so that the antibodies of CRP and SAA were modified on the substrate surface. Then substrate combined with antigen to form the bottom layer of the sandwich. 4-Mercaptobenzoic acid (4-MBA) and Nairn blue chloride (NBA) were used as signalling molecule probes. Hollow gold nanoparticles (HGNPs) modified with (EDC / NHS) enabled the attachment of antibodies to CRP and SAA to the surface of HGNPs. Eventually, the complex formed by the antigen-antibody reaction reacted with the sandwich substrate after the modification reaction to form the final "sandwich" structure. We successfully applied it to the specific detection of inflammatory markers in fascial tissue of TLF. The results showed that it had high stability and repeatability, which can bring some help for clinical work.
研究领域:
生物传感器
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医学诊断
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纳米技术
·
炎症标志物
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产品关联
DOI
高影响因子
Biomaterials Advances
·
2024
·
IF 5.5
Xiaomei Dai,Meijuan Yuan,Yuxia Yang,Mengbo Dang,Jian Yang,Junli Shi,Dianwei Liu,Mingjun Li,Hang Yao,Wenyong Fei
The tendon-bone interface heals through scar tissue, while the lack of a natural interface gradient structure and collagen fibre alignment leads to the occurrence of retearing. Therefore, the promotion of tendon healing has become the focus of regenerative medicine. The purpose of this study was to develop a gradient COL1/ hydroxyapatite (HAp) biomaterial loaded with human amniotic mesenchymal stem cells (hAMSCs). The performance of common cross-linking agents, Genipin, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/N-hydroxysuccinimide (EDC/NHS), and dual cross-linked materials were compared to select the best cross-linking mechanism to optimize the biological and mechanical properties of the scaffold. The optimal COL1/HAp-loaded with hAMSCs were implanted into the tendon-bone rotator cuff interfaces in rats and the effect on the tendon-bone healing was assessed by micro-CT, histological analysis, and biomechanical properties. The results showed that Genipin and EDC/NHS dual cross-linked COL1/HAp had good biological activity and mechanical properties and promoted the proliferation and differentiation of hAMSCs. Animal experiments showed that the group using a scaffold loaded with hAMSCs had excellent continuity and orientation of collagen fibers, increased fibrocartilage and bone formation, and significantly higher biomechanical functions than the control group at the interface at 12 weeks post operation. This study demonstrated that dual cross-linked gradient COL1/HAp-loaded hAMSCs could promote interface healing, thereby providing a feasible strategy for tendon-bone interface regeneration.
研究领域:
再生医学
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组织工程
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生物材料
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干细胞生物学
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骨科
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
·
2018
·
IF 5.089
Liming Ge,Mingjin Zhu,Xinying Li,Yongbin Xu,Xiaonan Ma,Rong Shi,Defu Li,Changdao Mu
It is significant to develop new approaches to incorporate naturally active agents into gelatin-based edible films for effective antioxidant and long-term antibacterial activities. Here the naturally active compound rosmarinic acid (RosA) was firstly conjugated onto gelatin backbone via EDC/NHS chemistry. Then the conjugates were plasticized by glycerol and crosslinked by dialdehyde xanthan gum to fabricate active RosA-gelatin edible films. The results indicate that the RosA-gelatin edible films present good water resistance property, strong mechanical property and excellent ultraviolet light barrier ability. The conjugation of RosA confers gelatin-based edible films with effective antioxidant and antibacterial activities as expected. It is interesting that RosA-gelatin edible films after 3 months of storage still show good antibacterial activity as that of newly prepared ones, demonstrating the long-term antibacterial activity. Overall, the fabricated RosA-gelatin edible films may have promising application in the fields of food and pharmaceutical packaging.
研究领域:
材料科学
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食品科学
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药学
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生物技术
✓ 已验证
产品关联
DOI
高影响因子
Military Medical Research
·
2023
·Vol. 10
·期 1
·1-15
·
IF 21.1
Xue Yao-Ting,Chen Ming-Yu,Cao Jia-Sheng,Wang Lei,Hu Jia-Hao,Li Si-Yang,Shen Ji-Liang,Li Xin-Ge,Zhang Kai-Hang,Hao Shu-Qiang,Juengpanich Sarun,Cheng Si-Bo,Wong Tuck-Whye,Yang Xu-Xu,Li Tie-Feng,Cai Xiu-Jun,Yang Wei
Background Reconstruction of damaged tissues requires both surface hemostasis and tissue bridging. Tissues with damage resulting from physical trauma or surgical treatments may have arbitrary surface topographies, making tissue bridging challenging.Methods This study proposes a tissue adhesive in the form of adhesive cryogel particles (ACPs) made from chitosan, acrylic acid, 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS). The adhesion performance was examined by the 180-degree peel test to a collection of tissues including porcine heart, intestine, liver, muscle, and stomach. Cytotoxicity of ACPs was evaluated by cell proliferation of human normal liver cells (LO2) and human intestinal epithelial cells (Caco-2). The degree of inflammation and biodegradability were examined in dorsal subcutaneous rat models. The ability of ACPs to bridge irregular tissue defects was assessed using porcine heart, liver, and kidney as the ex vivo models. Furthermore, a model of repairing liver rupture in rats and an intestinal anastomosis in rabbits were established to verify the effectiveness, biocompatibility, and applicability in clinical surgery.Results ACPs are applicable to confined and irregular tissue defects, such as deep herringbone grooves in the parenchyma organs and annular sections in the cavernous organs. ACPs formed tough adhesion between tissues [(670.9 ± 50.1) J/m2 for the heart, (607.6 ± 30.0) J/m2 for the intestine, (473.7 ± 37.0) J/m2 for the liver, (186.1 ± 13.3) J/m2 for muscle, and (579.3 ± 32.3) J/m2 for the stomach]. ACPs showed considerable cytocompatibility in vitro study, with a high level of cell viability for 3 d [(98.8 ± 1.2) % for LO2 and (98.3 ± 1.6) % for Caco-2]. It has comparable inflammation repair in a ruptured rat liver (P = 0.58 compared with suture closure), the same with intestinal anastomosis in rabbits (P = 0.40 compared with suture anastomosis). Additionally, ACPs-based intestinal anastomosis (less than 30 s) was remarkably faster than the conventional suturing process (more than 10 min). When ACPs degrade after surgery, the tissues heal across the adhesion interface.Conclusion sACPs are promising as the adhesive for clinical operations and battlefield rescue, with the capability to bridge irregular tissue defects rapidly.
研究领域:
生物医学工程
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组织工程
·
外科材料
·
伤口愈合
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生物材料
✓ 已验证
DOI
PubMed
高影响因子
POLYMERS FOR ADVANCED TECHNOLOGIES
·
2018
·Vol. 29
·期 12
·3159-3170
·
IF 2.137
Kai Huang,Xiao Quan,Xipeng Li,F. Handan Tezel,Baoan Li
Organic fouling has been a major impediment for the widespread application of nanofiltration membranes because it could degrade the performance and shorten the service life of such membranes. In this study, the NH 2 -functionalized silica nanoparticles (NH 2 –SiO 2 NPs) were grafted on the surface of commercial NF90 membrane to improve the hydrophilicity and antifouling property with the N -(3-dimethylaminopropyl)- N -ethylcarbodiimide hydrochloride coupled with the N -hydroxysuccinimide (EDC/NHS) to activate carboxyl groups on the membrane surface. The surface roughness (Ra) of this modified membrane decreased from value of 47.4 nm of the pristine membrane to 43 nm. The water contact angle of the modified membrane decreased from 48.4° to 15°, indicating the high hydrophilicity of the surface. Moreover, the EDC/NHS membrane showed good stability when it was exposed to the ruinous physical stress (2-min sonication). The optimized grafting conditions, including the SiO 2 concentration (0.5 wt%), the activation time of EDC/NHS (20 min), and the reaction time of NH 2 –SiO 2 NPs with membrane surface (12 h), were obtained by a series of experiments. Under the antifouling test of 36 hours with bovine serum albumin (BSA) and hexadecyltrimethylammonium bromide (CTAB) as foulants, the EDC/NHS membrane lost 42.1% and 49.6% of its initial flux, respectively, which is lower than that of the pristine membrane (52.2% and 68%, respectively). After cleaning with deionized water for 2 hours, the EDC/NHS membrane also showed significant improvement of flux recovery ratio (92% and 87%, respectively) than did the pristine membrane (70.3% and 60%, respectively).
研究领域:
环境工程
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膜科学
·
水处理
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产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Weijun He,Haofei Li,Xinbin Xu,Xing Zhang,Junlin Chen,Chuhan Lv,Hongbo Yu,Qi Feng,Hua Dong
Islets transplantation is a promising method to treat type 1 diabetes. Unfortunately, current islet encapsulation and transplantation strategies are difficult to simultaneously achieve the minimally invasive transplantation, rapid mass transportation, accurate positioning, long-term stability and easy retrieval. Herein, we present a novel strategy for islet encapsulation and transplantation that integrates the advantages of microencapsulation and macroencapsulation. Specifically, islets are first encapsulated in methacrylated carboxymethyl chitosan (CMCSMA) microgels by droplet microfluidic technology, and then oxidized sodium alginate-succinimide ester (OSA-NHS) as the assembling agent is added to time-sequentially assemble the islets-laden microgels. The as-formed microgel assembly (MA-CHO/NHS), or namely bioartificial pancreas, is injectable before transplantation due to the quick formation of dynamic Schiff base bonds, and becomes quite stable after transplantation with the slow formation of stable amide bonds. In addition, the interconnected micropores inside the microgel assembly is beneficial for mass transportation, and the abundant aldehyde and succinimide ester groups on assembling agent ensures good tissue adhesion. This injectable, tissue-adhesive, microporous, positionable and retrievable bioartificial pancreas can reverse the blood glucose levels of diabetic mice from hyperglycemia to normoglycemia for at least 90 days, and thus shows a broad application prospect in the field of diabetes treatment.
研究领域:
生物医学工程
·
糖尿病研究
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组织工程
·
生物材料
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产品关联
DOI
高影响因子
Advanced Healthcare Materials
·
2025
·
IF 9.6
Mingshan Zhang,Shi-yao Zhang,Huiqin Zhang,Youwei Liu,Yipeng Dong,Daobo Han,Le Chang,Ning Yang,Jianguo Tian,Yan Wang,Qing Ye
Reproducing the microstructure of the natural cornea remains a significant challenge in achieving the mechanical and biological functionality of artificial corneas. Therefore, the development of cascade structures that mimic the natural extracellular matrix (ECM), achieving both macro-stability and micro-structure, is of critical importance. This study proposes a novel, efficient, and general photo-functionalization strategy for modifying natural biomaterials. Collagen microfibers obtained through electrospinning are functionalized with an active N-Hydroxysuccinimide (NHS) ester, to impart light-curing ability. This approach expands the construction of photo-controllable hydrogel systems beyond conventional single methacrylate (MA) modifications or di-tyrosine bonding, enabling integration with other biomaterials for comprehensive ECM remodeling. Subsequently, the collagen microfibers are then photo-embedded into gelatin methacryloyl (GelMA) via covalent crosslinking to form a fibrous hydrogel, which supports both structural and functional requirements. In terms of biological functionality, the hydrogel promotes significant inward migration and retention of human corneal fibroblasts (hCFs), replicating ECM-like environments. Furthermore, its excellent burst resistance suggests potential as a bioadhesive. In a rabbit model, the hydrogel achieved effective repair of large-sized (6 mm) corneal defects, facilitates epithelial migration, and maintained long-term stability. This work provides valuable guidance for designing efficient and simplified bioactive materials for corneal repair and broader tissue engineering applications.
研究领域:
组织工程
·
生物医学工程
·
材料科学
·
眼科
·
再生医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2021
·
IF 13.273
Wan Peng,Hua Yin,Peiming Liu,Jiangmei Peng,Jin Sun,Xiao Zhang,Yahui Gu,Xiaohan Dong,Zhuangzhuang Ma,Jian Shen,Pingsheng Liu
Biofilm formation on biomedical devices & implants and the resulting bacterial induced infections are remaining severe clinic issues. Here, a facile layer-by-layer strategy to covalently construct poly(hexamethylene biguanide) (PHMB) as the high-efficiency bactericidal coating on silicone rubber (SR) has been developed. Based on the NHS/NH 2 chemistry (the reaction between active ester groups and amino groups), an active ester homo-polymers (pNHSMA) as the precursor layer was created on activated SR surface (SR-NH 2 ), which followed by the immobilization of dense PHMB coating. XPS and water contact angle tests verified the successful construction of PHMB coating on the surface. Robust relationships between the contact time/initial seeding density of bacteria and the bactericidal capacity of the coating have been established based on systematical qualitative and quantitative antibacterial evaluations. Results showed that the PHMB coating is able to kill 100% of the attached S. aureus cells within 1 h with initial density below 1.18 × 10 5 CFU/cm 2 without compromising of the cytocompatibility of the substrates. Interestingly, the coating significantly increased the mechanical property of the SR substrates due to the additional crosslinking introduced by the coating. Furthermore, the high-efficiency antibacterial property of PHMB coating (bactericidal ratio of 96.83%) and the significant suppressed implant-induced infection have been verified via the in vivo simulated infection model on rats. In the meantime, this surface modification strategy can be easily applied to SR based catheters, which is able to completely kill the surface attached bacteria when immersed in a bacterial suspension with a density of 3 × 10 6 CFU/mL for 30 min. These findings provide a new approach to combat the biofilm formation and devices-associated infections for general SR based biomedical devices/implants.
研究领域:
材料科学
·
生物医学工程
·
生物材料
·
感染控制
✓ 已验证
产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2021
·
IF 4.821
Yi Zhang,Yuanxiang Huang,Zhenfeng Yue,Huajun Fan,Shaojing Wu
A novel, efficient and specific aptamer-functionalized sorbent was successfully synthesized and applied in the analysis of ultra-trace aflatoxin M1 (AFM1) and its analogues in milk. By using a gold nanoparticle-based colorimetric analysis tool, we could determine that the AFM1-aptamer (M1-aptamer) recognized and bound AFM1 to generate an aptamer-target complex. After analysis and verification, the M1-aptamer on the surface of the carboxyl microspheres were completely modified via EDC/NHs amide formation. The effects of extraction pH, extraction buffer, concentration of Mg 2+ and elution solvents were investigated by single-factor experiments to determine the most suitable analysis conditions. Using these conditions, the extraction performance of the M1-aptamer-functionalized sorbent could be studied and an analytical method for AFM1 and its analogues in milk could be established. The results of adsorption equilibrium as well as adsorption selectivity experiments indicated that the M1-aptamer offered a specific adsorption site to selectively adsorb aflatoxins exhibiting a dihydrofuran oxaphthalene scaffold. The adsorption capacity of the aptamer-functionalized sorbent was 233.1 μg/g. A studied method for the determination AFM1 in milk with low matrix effect demonstrated a good linear correlation in the range of 0 to 2.0 μg/L (R 2 = 0.9982) and high detection sensitivity (detection limits of 7 ng/kg in milk and 21 ng/kg in milk power). Furthermore, excellent spiked recovery and good repeatability were obtained. The method delivered specific, accurate and reproducible results for enriching and detecting ultra-trace AFM1 and its analogues in milk.
研究领域:
分析化学
·
食品科学
·
纳米技术
·
毒理学
✓ 已验证
产品关联
DOI
高影响因子
POLYMER ENGINEERING AND SCIENCE
·
2016
·Vol. 56
·期 12
·1432-1436
·
IF 1.719
Shiping Zhan,Nan Liu,Weijing Wang,Shuhua Chen,Jingchang Wang
To improve the properties of chitosan (CS) and poly(L-lactic acid) (PLLA) and obtain fully biodegradable materials, CS- g -PLLA copolymers were prepared using 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC)/N-hydroxyl succinimide (NHS) as a coupling agent. The copolymers were characterized by Fourier transform infrared analysis (FTIR), 1 H nuclear magnetic resonance ( 1 H NMR), elemental analysis, differential scanning calorimetry (DSC), X-ray diffraction (XRD) and scanning electron microscopy (SEM). The results obtained by FTIR and 1 H NMR showed that CS and PLLA were grafted successfully via an amide bond. DSC and XRD results showed that the thermal stability of CS had been significantly improved by grafting PLLA to the molecular chains of CS and the crystallinity of the CS- g -PLLA copolymers decreased significantly. Elemental analysis showed that the achieved the maximum degree of substitution of PLLA was 60.88%, while the concentration of CS was 2 mg/mL, the PLLA molecular weight was 10,000, and the EDC/NHS ratio was 2:1. Images from SEM demonstrated that the copolymers had a spherical shape and smooth surface. Moreover, the products were well dispersed without any aggregation. POLYM. ENG. SCI., 56:1432–1436, 2016. © 2016 Society of Plastics Engineers
研究领域:
高分子科学
·
材料科学
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）