ACS Applied Materials & Interfaces
·
2013
·
IF 5.008
Yi Gong,Ai Mei Zhu,Qiu Gen Zhang,Mei Ling Ye,Hai Tao Wang,Qing Lin Liu
Cell encapsulation by locking the interfacial microgels in a water-in-oil Pickering emulsion has currently been attracting intensive attention because of the biofriendly reaction condition. Various kinds of functional microgels can only stabilize an oil-in-water Pickering emulsion, and it is thus difficult to encapsulate cells in the emulsion where the cells are usually dispersed in the continuous phase. Herein, we introduce a facile method for preparing cell-embedded colloidosomes in an oil-in-water emulsion via polyelectrolyte complexation. Escherichia coli (E. coli) was chosen as a model cell and embedded in the thin shell of chitosan/poly(N-isopropylacrylamide-co-acrylic acid) (P(NIPAM-co-AAc)) microcapsules. This is beneficial for expressing cell function because of the little resistance of mass exchange between the embedded cells and the external environment. Cells can be used in biocatalysis or biomedicine and our product will hold great promises to improve the performance in those fields. The synthesis route presents a platform to prepare cell-embedded microcapsules in an oil-in-water Pickering emulsion in a facile and biocompatible way. First, an emulsion stabilized by P(NIPAM-co-AAc) microgels was prepared. Then, the interfacial microgels in the emulsion were locked by chitosan to form colloidosomes. The mechanism of cell encapsulation in this system was studied via fluorescent labeling. The viability of E. coli after encapsulation is ca. 90%. Encapsulated E. coli is able to metabolize glucose from solution, and exhibits a slower rate than free E. coli. This demonstrates a diffusion constraint through the colloidosome shell.
研究领域:
生物技术
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材料科学
·
化学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2016
·
IF 4.758
Xiaobing Tang,Aihua Sun,Chengyi Chu,Chongyang Wang,Zhenzhong Liu,Jianjun Guo,Gaojie Xu
Multiresponsive photonic hydrogels (MRPHs) were prepared by magnetically controlled self-assembly of Fe 3 O 4 @C nanoparticles and these are sensitive to temperature, humidity, compression, tension and ethanol. This exhibits brilliant structural color thanks to the combination of AM and NIPAM monomers. Along with the increase of compression and tension on the MRPHs, the reflection peak of the samples blue shifts. When exposed MRPHs in environments with different humidity, its structure color red shifts as the humidity increases from 33% RH to 95% RH. Besides, the reflection peak redshift was observed when the MRPHs was immersed in absolute ethyl alcohol. This work may provide a new way to prepare smart photonic hydrogel with integrated functions of mechano-, humidity-, temperature- and solvent- responses.
研究领域:
材料科学
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生物医学工程
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产品关联
DOI
高影响因子
MACROMOLECULAR RESEARCH
·
2013
·Vol. 21
·期 12
·1338-1348
·
IF 1.639
Xu Yiting,Xie Jianjie,Chen Lingnan,Yuan Conghui,Pan Yinyin,Cheng Ling,Luo Weiang,Zeng Birong,Dai Lizong
A novel organic/inorganic hybrid amphiphilic random copolymer poly(methacrylate isobutyl POSS- co -N-isopropylacrylamide- co -oligo(ethylene glycol) methyl ether methacrylate- co -2-vinylpyridine), poly(MAPOSS- co -NIPAM- co -OEGMA- co -2VP), was synthesized via reversible addition-fragmentation chain transfer (RAFT) polymerization. The self-assembly behavior of random copolymers in aqueous solution was investigated by dynamic light scattering (DLS) as well as transmission electron microscopy (TEM). The results indicate the novel random copolymer in water could self-assemble into spherical aggregates and the self-aggregate size displays a remarkable dependence on pH. The stimuli-responsive characteristics of these assembles were tested by means of UV-vis spectra, DLS and TEM. There is a critic Zn 2+ concentration over which the aggregates can be coordinated into well-define spherical aggregate clusters. The critic Zn 2+ concentration can be tuned finely through adjusting solution concentration or 2VP amount. Results from UV-vis and DLS reveal that the copolymer solutions exhibit a sharp and intensive lower critical solution temperature (LCST). Some factors such as the solution concentration, molecular weight, pH and copolymer generation, which could affect the cloud point, were studied systematically. The essentially predetermined LCST can be achieved by altering the content of 2VP or pH. In addition, these novel hybrid aggregates can undergo an association/disassociation cycle with the heating and cooling of solution and the degree of reversibility shows a strong concentration dependence. As a novel organic-inorganic hybrid material which can respond to multiple external stimuli including temperature, pH, metal ions with sharp stimuli-responsive behaviors, it is potentially used for biomedicine, catalysis, diagnostics, bioseparations, biosensors and for fundamental investigation.
研究领域:
材料科学
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高分子化学
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生物医药
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产品关联
DOI
高影响因子
Journal of Water Process Engineering
·
2025
·
IF 6.7
Lian Yang,Haoran Qiu,Lijun Zhao,Ping Xiao,Guoliang Liu,Jiang Chang,Tianbei Zhang,Shaoxia Yang,Feng Xiao
Polyvinylidene fluoride (PVDF)-N-isopropylacrylamide (NIPAM)-Graphene oxide (GO) (PNG) membrane can dramatically change their microstructure and performance in response to temperature variations in raw water. However, the research on the effect of responsible temperature and individual component in this membrane is lack of clarity. In this study, the permeability-selectivity index was established to determine the responsible temperature of the PNG membrane at 35 °C (PNG-35). And the biofilter effluent from Beijing wastewater reclamation treatment plant was used to investigate the contribution of each composition in the PNG membrane. It can be noticed that with the addition of GO could increase the permeability 23.33 %，while selectivity increased 15.05 %. After the further introduction of NIPAM, subsequently the permeability could increase 82.86 % with the selectivity increased 11.35 %. Finally, comparing the effluent of membrane by EEM, molecular weight distribution and FTICR-MS, the results showed that the humic-like substances were the main contaminants with molecular weights of 142 Da, 397 Da, and 1147 Da, characterized by their sulfur content, hydrophilicity, and negative charged. Humic-like substances were the main contaminants which the sequence of removal was PNG-35 > PNG > PG > PVDF. Apparently, the adding of GO, NIPAM and the responsible temperature could improve the removal of humic-like substances that attributed to the R ir decreased the value at 0.201, 0.092 and 0.041. And charge effects between the contaminants and membrane surface played a significant role under the responsible temperature.
研究领域:
环境科学
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材料科学
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水处理技术
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膜技术
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高分子化学
✓ 已验证
产品关联
DOI
高影响因子
MACROMOLECULES
·
2018
·
IF 5.914
Jinqiao Xue,Wei Bai,Hanyi Duan,Jingjing Nie,Binyang Du,Jing Zhi Sun,Ben Zhong Tang
We studied the aggregation-induced emission (AIE) phenomenon in a nanoconfined environment, where the AIE-active molecule, namely, 1,1,2,2-tetrakis(4-methanoylphenyl)ethane (TPE-4ALD), was held in space via four acylhydrazone bonds within the thermosensitive microgel networks. The thermosensitive microgels, namely N-AH-TPE, were synthesized via the copolymerization of N-isopropylacrylamide (NIPAM) and 4-acylhydrazine-(2-hydroxy-3-(methacryloxypropyl)pyridine hydrochloride (AH monomer) with TPE-4ALD as cross-linker via surfactant free emulsion polymerization (SFEP) in aqueous solution at 70 °C. Acylhydrazone-bonded tetraphenylethene (TPE-4AH) moieties were thus constructed and worked as the fluorophore in N-AH-TPE microgels. The aqueous suspensions of N-AH-TPE microgels exhibit strongly bluish-green fluorescence under ultraviolet excitation because the four arms of TPE-4AH moieties were held and their intramolecular motions are strongly restricted. It is estimated that there is one TPE-4AH moiety per about 394 nm3 for the swollen N-AH-TPE microgels. The fluorescent properties of N-AH-TPE microgels can be modulated via the change of hydrophilic and hydrophobic environments of TPE-4AH moieties exerted by external stimuli, like addition of various good solvents for TPE-based structures, i.e., N,N-dimethylformamide (DMF), methanol, ethanol, tetrahydrofuran (THF), and N,N-dimethyl sulfoxide (DMSO), varying the solution temperature as well as the counteranions of the microgels. An unusual enhancement in the fluorescent intensity is observed when specific amounts of organic solvent are added into the aqueous suspensions of N-AH-TPE microgels, which can be attributed to the cononsolvency of the polyNIPAM network chains. The shrinkage of N-AH-TPE microgels caused by the cononsolvency effect further strengthens the confinement of TPE-4AH moieties and hence enhances the fluorescent emission of the microgels even though the organic solvents added are good solvents for TPE-4AH. Increasing the solution temperature of N-AH-TPE microgels or introducing hydrophobic counteranions into the microgels also significantly enhances the fluorescent emission of the microgels.
研究领域:
材料科学
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化学
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高分子科学
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纳米技术
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光学
✓ 已验证
产品关联
DOI
高影响因子
ACS Biomaterials Science & Engineering
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2018
·
IF 4.432
Lixia Yu,Anjie Dong,Ruiwei Guo,Muyang Yang,Liandong Deng,Jianhua Zhang
Chemo-photothermal therapy has shown enormous potential in treating cancer. To achieve the chemo-photothermal synergistic effect, an efficient nanoparticulate system with the ability for simultaneous codelivery of chemotherapeutic drug and photothermal agent as well as photothermal-triggered drug release is highly desirable. Herein, an in situ polymerization within liposome template was designed to prepare liposome-coated poly(N-isopropylacrylamide-co-acrylamide) (P(NIPAM-co-AAM)) nanogels, which can efficiently coencapsulate a NIR dye indocyanine green (ICG) and high amount of doxorubicin hydrochloride (DOX). The DOX/ICG coloaded hybrid nanogels, denoted as DI-NGs@lipo, integrated the desirable functions of PEGylated liposomes and thermosensitive nanogels. The PEGylated liposome shell provided excellent storage stability, hemodynamic stability, and fluorescence stability. Meanwhile, the thermosensitive P(NIPAM-co-AAM) nanogels core endowed DI-NGs@lipo with volume phase transition temperature (VPTT) at about 40 °C, allowing for thermo-controlled transformation and drug release. The significant photothermal effect of DI-NGs@lipo and the simultaneous hyperthermia-triggered DOX release were observed under NIR light irradiation. The DI-NGs@lipo was demonstrated to be uptaken by 4T1 murine breast cancer cells via endocytosis, enhancing the distribution of DOX in the cell nucleus. Compared with chemo or photothermal treatment alone, the combination treatment of DI-NGs@lipo with NIR light irradiation induced significantly higher cytotoxicity to 4T1 cells, demonstrating the chemo-photothermal synergistic therapeutic effects on tumor cells. In a word, the strategy provided here offers a facile approach to develop a multifunctional nanoplatform for codelivery of DOX and ICG, which can synergistically improve the cancer-cell-killing efficiency, demonstrating great potential in chemo-photothermal therapy.
研究领域:
纳米技术
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癌症治疗
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药物递送系统
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光热疗法
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Yang Pang,Zhichuang Qi,Liangliang Cui,Zhangmi Huang,Chunyan Hu,Zhijia Zhu,Baojiang Liu
Photonic crystal hydrogels (PCHs) are innovative materials that translate imperceptible deformations and humidity changes into visible colors, broadening the applications of photonics in bioengineering and smart materials. To overcome poor mechanical properties of traditional PCHs limited by weak intermolecular forces, we designed a PCH with a dual-network framework comprising N-isopropylacrylamide-co-acrylamide (NIPAM-co-AM) and biomass lotus root starch (LR). Since LR is rich in hydroxyl groups, it can undergo molecular linkage entanglement with the NIPAM-co-AM hydrogel matrix, forming hydrogen bonds that significantly enhance the mechanical properties of the PCH. We have prepared PCH films capable of conveying multidimensional information about the extent and distribution of transient deformation by encapsulating magnetic photonic crystal microspheres (MPCMs) within a hydrogel matrix. The PCH films were found to have ultrafast response time (< 10 s), full-color tunable range, high spatial resolution, excellent mechanical toughness (tensile up to 0.17 MPa) and sensitivity (2.52 nm % −1 ), and were able to sense relative humidity (RH) from 11 % to 98 %. Equipped with a dynamically reconfigurable lattice, this dual-responsive (tensile/humidity) PCH not only facilitates the creation of water-rewritable photonic films but also holds promise for integration into structural color elastic fabrics, thereby presenting novel prospects for smart textile applications.
研究领域:
材料科学
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生物工程
·
智能材料
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纺织工程
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光子学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry C
·
2016
·Vol. 4
·期 29
·7083-7092
·
IF 5.066
Yajiao Song,Jianhua Lü,Bingxin Liu,Changli Lü
Thermo-responsive block copolymer brushes of PNIPAM-b-P(MQ-co-GMA) were successfully grafted from graphene oxide (GO) by consecutive reversible addition–fragmentation chain transfer (RAFT) polymerization based on the monomers of N-isopropylacrylamide (NIPAM), 5-(2-methacryloyl-ethyloxymethyl)-8-quinolinol (MQ) and glycidyl methacrylate (GMA). The 8-hydroxyquinoline units in the polymer brushes could coordinate with Al3+ to form green luminescent copolymer brushes containing tris(8-hydroxyquinoline)aluminum (Alq3) on the GO surface. The resulting fluorescent hybrid could be used as a nano-platform for the sensitive and robust detection of 2,4,6-trinitrophenol (TNP). The green fluorescence of the polymer brush modified GO hybrid in aqueous solution could be quenched by TNP via charge transfer and the observed linear fluorescence intensity change allowed the quantitative detection of TNP with a detection limit down to 2.38 × 10−9 M. The fluorescent nanohybrids exhibited a robust temperature-responsive behavior as a result of the conformation change in PNIPAM chains of polymer brushes.
研究领域:
材料科学
·
高分子化学
·
纳米技术
·
分析化学
·
环境科学
✓ 已验证
DOI
高影响因子
Cell Reports Physical Science
·
2024
·
IF 7.9
Zhongwu Bei,Lin Ye,Qi Tong,Yang Ming,Tingyu Yang,Yuzhou Zhu,Linghong Zhang,Xicheng Li,Hanzhi Deng,Jie Liu,Wen Chen,Bingyang Chu,Zhiyong Qian
Localized hyperglycemia, chronic inflammation, and recurrent infections severely impair wound healing in diabetes. Traditional wound dressings that passively promote healing maintain wound moisture with only limited efficacy, while microbial infections further complicate the healing process. Herein, we develop a thermostimulated shrinking and adhesive hydrogel dressing for chronic wound healing composed of N-isopropylacrylamide (NIPAM), polyacrylic acid grafted with N-hydroxysuccinimide ester, dopamine-modified gelatin (GelDA), and silver-coated clay-tannic acid nanoparticles (Ag@Clay-TA), integrating properties such as tissue adhesion and antibacterial and antioxidant functions. Notably, it adheres to skin wounds, actively contracts the wound in response to skin temperature, and releases antibacterial nanoparticles. In vivo analyses confirm that this hydrogel effectively eliminates bacteria at the wound site, reduces inflammation and oxidative stress, and promotes rapid wound closure and healing. This hydrogel dressing shows promising potential in alleviating inflammation and accelerating wound closure in chronic wounds infected with methicillin-resistant Staphylococcus aureus (MRSA).
研究领域:
材料科学
·
生物医学工程
·
伤口愈合
·
糖尿病研究
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）