BIOSENSORS & BIOELECTRONICS
·
2013
·
IF 5.437
Hongqi Chen,Fei Yuan,Shaozhen Wang,Juan Xu,YiYan Zhang,Lun Wang
In this work, we design a FRET system for sensitive and selective determination of thrombin in red region, in which NaYF 4 :Yb,Er upconversion nanoparticles (UCNPs) act as donor and gold nanorods (Au NRs) act as acceptor. NaYF 4 :Yb,Er UCNPs with a strong emission at 661 nm were successfully synthesized by tuning the doped ions ratio. Carboxyl-functionalized NaYF 4 :Yb,Er UCNPs and Au NRs were then prepared and conjugated with the thrombin aptamers, respectively. The fluorescence emission band of NaYF 4 :Yb,Er UCNPs ( λ max =661 nm) highly overlaps with the absorption band of Au NRs( λ max =666 nm), which benefits from the large tunability of the spectrum band of Au NRs. A FRET system was then formed when thrombin was added to the mixture of NaYF 4 :Yb,Er UCNPs and Au NRs, which were both modified thrombin aptamers. The fluorescence quenching efficiency of NaYF 4 :Yb,Er UCNPs was increased in a thrombin concentration-dependent manner, which built the principle of thrombin quantification. The linear range was 2.5–90 nM in an aqueous buffer, and 3.75–112.5 nM in spiked human serum samples for thrombin. It also demonstrates a high selectivity to other biological species due to the specific binding. The measurement of thrombin in human plasma is satisfying, suggesting that the FRET system is of practical value in a complex biological sample matrix in red region.
研究领域:
分析化学
·
生物传感
·
纳米技术
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2013
·Vol. 3
·期 33
·13915-13925
·
IF 2.562
Leyong Zeng,Lingchao Xiang,Wenzhi Ren,Jianjun Zheng,Tianhua Li,Bin Chen,Jichao Zhang,Chengwen Mao,Aiguo Li,Aiguo Wu
Due to non-invasive deep imaging and therapy, multifunctional agents of magnetic resonance (MR)/upconversion luminescence (UCL) imaging and photodynamic therapy (PDT) play an important role in clinical diagnosis and treatment of cancers, and also in the assessment of therapy effect. In this paper, tetra-sulfonic phthalocyanine aluminium (AlPcS4) photosensitizers-conjugated Fe3O4@NaYF4:Yb/Er (NPs-AlPcS4) nanocomplexes were synthesized for the T2-weighted MR/UCL imaging and PDT of cancer cells. The PEG-coated Fe3O4@NaYF4:Yb/Er nanoparticles (NPs) with a core–shell structure showed strong T2-weighted MR relaxivity (r2 = 42.131 mM−1 s−1) and UCL emission in the visible region (the bands at about 654–674 nm, 545 nm and 524 nm), and were conjugated successfully with AlPcS4 photosensitizer by electrostatic interaction. By direct observation of XFM and staining with Prussian blue, the element distribution and location of NPs in MCF-7 cells were characterized, respectively. Under irradiation from a 980 nm laser, the death ratio of MCF-7 cells incubated with NPs-AlPcS4 nanocomplexes could be up to about 70%. The results indicated that the as-prepared NPs-AlPcS4 nanocomplexes would be a potential candidate as multifunctional nanoprobes for the dual-modal T2-weighted MR/UCL imaging and PDT of cancers in the future.
研究领域:
生物医学工程
·
肿瘤学
·
材料科学
·
影像医学
✓ 已验证
DOI
高影响因子
RSC Advances
·
2017
·Vol. 7
·期 60
·37929-37937
·
IF 3.108
Yang Luo,Sinan Du,Wei Zhang,Zhengfang Liao,Fang Zuo,Shengtao Yang
Core@shell structures of Fe3O4@Mn2+-doped NaYF4:Yb/Tm nanoparticles (NPs) with multifunctional properties were prepared using a hydrothermal route with a seed-growth procedure. The effect of Mn2+ ions on the phase, shape, and upconversion luminescence (UCL) of Fe3O4@Mn2+-doped NaYF4:Yb/Tm NPs was explored using X-ray diffraction, transmission electron microscopy, dynamic light scattering, and photoluminescence spectroscopy. Furthermore, hydrophobic to hydrophilic surface modification of the Fe3O4@Mn2+-doped NaYF4:Yb/Tm NPs was achieved by coating the NPs with an amphiphilic polymer (poly(maleic anhydride-alt-1-octadecene) (C18PMH)) modified with amine-functionalized methyl ether poly(ethylene glycol). Then, the NIR-to-NIR UCL ascribed to the Tm3+ ions, T1-weighted MRI ascribed to the Mn2+ ions, and T2-weighted MRI ascribed to the Fe3O4 core of the hydrophilic Fe3O4@Mn2+-doped NaYF4:Yb/Tm NPs were evaluated. The results indicate that Mn2+ doping is an effective method to control the size/shape and improve the UCL intensity of Fe3O4@Mn2+-doped NaYF4:Yb/Tm NPs, which are promising as imaging agents for NIR-to-NIR UCL and T1/T2-weighted MRI in biomedical research.
研究领域:
生物医学工程
·
材料科学
·
影像医学
✓ 已验证
DOI
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2017
·Vol. 409
·期 10
·2675-2683
·
IF 3.431
Gao Ni,Ling Bo,Gao Zhongling,Wang Lun,Chen Hongqi
An electrochemiluminescence resonance energy transfer (ECL-RET) system that detects prostate-specific antigen (PSA) was created. Near-infrared (NIR)-emitting NaYF 4 :Yb,Tm/Mn upconverting nanoparticles (UCNPs) are used as donors, and gold nanorods (GNRs) are used as acceptors. The ECL was enhanced nearly threefold by Mn 2+ doping, with an emission peak appearing at an NIR wavelength of 808 nm. Anti-PSA 1 (Ab 1 ) was bound to the surfaces of UCNPs after being modified with poly(acrylic acid) (PAA). As for acceptors, cetyltrimethylammonium bromide (CTAB)-capped GNRs were treated with 11-mercaptoundecanoic acid (MUDA) and then conjugated with Anti-PSA 2 (Ab 2 ). When PSA was added, donors and acceptors were brought in close proximity through specific interactions of antibodies and antigens, resulting in high quenching efficiency levels. Under optimal conditions, the linear range of detection was 3.75–938 pg/mL for PSA ( R = 0.999), with a detection limit as low as 3.16 pg/mL. This method can be applied to detect PSA in human serums with satisfactory results.
An electrochemiluminescence resonance energy transfer system was developed for determination of prostate-specific antigen using near-infrared-emitting NaYF 4 :Yb,Tm/Mn upconverting nanoparticles as donors and gold nanorods as acceptors.
研究领域:
分析化学
·
纳米技术
·
生物传感器
·
癌症诊断
✓ 已验证
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2016
·
IF 2.653
Keyi Cheng,Jianguo Zhang,Liping Zhang,Lun Wang,Hongqi Chen
A highly sensitive luminescent bioassay for the detection of Salmonella typhimurium was fabricated using Mn 2 + -doped NaYF 4 :Yb,Tm upconversion nanoparticles (UCNPs) as the donor and gold nanorods (Au NRs) as the acceptor and utilizing an energy transfer (LET) system. Mn 2 + -doped NaYF 4 :Yb,Tm UCNPs with a strong emission peak at 807 nm were obtained by changing the doped ion ratio. Carboxyl-terminated Mn 2 + -doped NaYF 4 :Yb,Tm UCNPs were coupled with S. typhimurium aptamers, which were employed to capture and concentrate S. typhimurium . The electrostatic interactions shorten the distance between the negatively charged donor and the positively charged acceptor, which results in luminescence quenching . The added S. typhimurium leads to the restoration of luminescence due to the formation of UCNPs-aptamers- S. typhimurium , which repels the UCNPs-aptamers from the Au NRs. The LET system does not occur because of the nonexistence of the luminescence emission band of Mn 2 + -doped NaYF 4 :Yb,Tm UCNPs, which had large spectral overlap with the absorption band of Au NRs. Under optimal conditions, the linear range of detecting S. typhimurium was 12 to 5 × 10 5 cfu/mL (R = 0.99). The limit of detection for S. typhimurium was as low as 11 cfu/mL in an aqueous buffer. The measurement of S. typhimurium in milk samples was satisfied in accordance with the plate-counting method, suggesting that the proposed method was of practical value in the application of food security.
研究领域:
生物传感器
·
微生物学
·
纳米技术
·
食品安全
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2017
·
IF 4.162
Liping Zhang,Bo Ling,Lun Wang,Hongqi Chen
An upconversion luminescence method was developed for the determination of glutathione (GSH), L -cysteine (Cys) or L -ascorbic acid (AA) based on redox reaction . We synthesized poly(acrylic acid) (PAA)-modified Mn 2+ -doped NaYF 4 :Yb,Tm upconversion nanoparticles (UCNPs), and the luminescence of these UCNPs was effectively quenched due to their carboxyl groups coordinating with Fe 3+ to form a UCNPs/Fe 3+ system. GSH, Cys or AA reduced Fe 3+ to Fe 2+ , which induced the luminescence recovery of the UCNPs. Under the optimized conditions, wide linear concentration ranges from 0.25-300 μM for GSH, 0.5–875 μM for Cys and 0.5–350 μM for AA were found, and the detection limits (3 S/K) were 0.2 μM, 0.5 μM and 0.2 μM, respectively. Thus, the UCNPs/Fe 3+ system was successfully applied for sensing GSH, Cys or AA.
研究领域:
纳米技术
·
分析化学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Nano Materials
·
2024
·
IF 5.3
Mingye Ding,Xiaoyan Wang,Yinbiao Shi,Zixia Lin,Zhengtao Deng,Xiaofei Yang
Owing to their excellent photoelectric and optical properties, lead halide perovskite quantum dots (PeQDs) have attracted great attention and have broad applications in various fields. However, the shortcomings of poor stability and short-wavelength (ultraviolet/visible) excitation restrict their practical applications. Herein, to address the above issues, we report a polyacrylonitrile-based composite film containing CsPbX3 (X = Br and I) PeQDs and β-NaYF4:Yb/Ln (Ln = Tm and Er) upconversion nanoparticles (UCNPs) via an electrospinning strategy. Interestingly, all-inorganic CsPbX3 PeQDs are synthesized through an in situ growth process in polyacrylonitrile nanofibers, which provide a polymer matrix for the protection of PeQDs against decomposition under the effect of an external environment. Upon 980 nm laser irradiation, lanthanide-sensitized UC luminescence of stable CsPbX3 can be realized through radiative energy transfer from Tm3+/Er3+ ions to PeQDs. Consequently, dual-mode luminescence, including downshifting and UC with color-tunable outputs, can be observed from PeQDs under dual-wavelength excitation (UV and NIR). Benefiting from the special luminescent feathers (temperature-sensitive UC and dual-mode luminescence), the as-obtained composite nanofibers exhibit great potential in high-level anticounterfeiting and accurate noncontact thermometry.
研究领域:
材料科学
·
纳米技术
·
光学
·
光子学
✓ 已验证
产品关联
DOI
高影响因子
Analytical Methods
·
2017
·Vol. 9
·期 22
·3296-3301
·
IF 1.9
Lei Wang,Yuan Ji,Yuezhen He,Lun Wang,Hongqi Chen
Li+-Doped NaYF4:Yb,Er upconversion luminescent nanoparticles (UCLNPs) were synthesized and modified with poly(acrylic acid) (PAA). The luminescence of the carboxyl-functionalized UCLNPs at 664 nm was quenched in the presence of cupric ions (Cu2+) due to the coordination of Cu2+ with the carboxyl group of PAA. When pyrophosphate ions (PPi) were added, the luminescence of the UCLNPs was restored due to the competitive binding of PPi with Cu2+. However, pyrophosphatase (PPase) could promote the hydrolysis of PPi, thus releasing Cu2+, leading to requenching of the luminescence. Under optimal conditions, the luminescence response was virtually proportional to the activity of the PPase between 0.500 and 50.0 mU mL−1, and the limit of detection was 0.441 mU mL−1. Based on the competition assay approach, a new highly sensitive method in the red region for real-time detection of PPase activity was developed.
研究领域:
分析化学
·
生物化学
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF RARE EARTHS
·
2017
·
IF 2.429
Xuhua LIANG,Jun FAN,Yongbo WANG,Yanyan ZHAO,Ruyi JIN,Tao SUN,Min CHENG,Xuejun WANG
In this paper, we demonstrated a one-step template-free strategy to fabricate a hollow mesoporous structured NaYF 4 :Yb,Er nanoparticles with excellent upconversion luminescence. Folic acid (FA), a commonly used cancer-targeting agent, was conjugated on the surface of the nanoparticles based on the presence of free amine groups, which were labeled as NaYF 4 :Yb,Er-FA HMUCNPs. The properties were extensively studied, which indicated the obtained samples showed a typical hollow mesoporous structure and excellent upconversion luminescence that were useful for cell imaging and drug delivery. The L929 cells viability, hemolysis assay and coagulation test demonstrated good biocompatibility of the samples. The anti-cancer drug doxorubicin hydrochloride (DOX) storage/release properties were demonstrated to be pH-responsive, in which, the drug release might be beneficial at the reduced pH for targeted release and controlled therapy. Moreover, it was found that DOX-loaded NaYF 4 :Yb,Er-FA HMUCNPs exhibited greater cytotoxicity to KB cells than free DOX due to the specific cell uptake by KB cells via folate receptor-mediate endocytosis. Therefore, the multifunctional nanoparticles combining upconversion luminescent property and hollow mesoporous structure have potential for simultaneous targeted anti-cancer drug delivery and cell imaging.
研究领域:
材料科学
·
纳米技术
·
生物医学工程
·
药学
·
癌症研究
✓ 已验证
产品关联
DOI
高影响因子
ACS Applied Nano Materials
·
2025
·
IF 5.5
Zhongfei Shang,Meimei Wang,Honghong Li,Jiahao Dong,Luyan Yang,Xincun Dou,Baiyi Zu
The exploration of efficient hazardous chemical sensing technology is of great importance to protect the environment and maintain public security. Here, a design strategy of a trimode upconversion nanoprobe was developed for H2O2 detection through establishing the inner filter effect (IFE) between the anthracene-based probes and the NaYF4:Yb/Tm/Er upconversion nanoparticles (UCNPs). The trimode nanoprobe exhibits superior H2O2 detection performance, including a low limit of detection (LOD) of 152 nM, fast response (<3 s), outstanding selectivity, and robust anti-interference ability even in the presence of 15 common interferents. Furthermore, the practical utility of this design is conclusively demonstrated through a sensing chip capable of accurately and selectively identifying H2O2 even in the presence of more than 7 types of coexisting multicolor interferents. This sensing strategy, characterized by its strong anti-interference capability even when facing extremely complex real-world samples, is expected to establish a promising framework for ultra-accurate detection of hazardous substances.
研究领域:
分析化学
·
纳米技术
·
传感器
·
环境监测
·
生物工程
✓ 已验证
产品关联
DOI
高影响因子
OPTICAL MATERIALS
·
2022
·
IF 3.754
Yinyin Chen,Xinying She,Zhouping Yang,Changfang Lu,Wei Zhang,Dongju Feng,Wei Wu,Xianxiang Wang
Doxorubicin hydrochloride (DOX), an anthraquinone-based antibiotic, is widely used in human tumors treatment. However, the residues of DOX in body would pose a threat to the health of heart and liver. Herein, a photochemical sensor NaYF 4 :Yb/Tm@NaYF 4 :Yb-Cit-CD (UCNPs) for detecting DOX has been developed in this paper. The fluorescence emission spectrum of UCNPs under 980 nm laser excitation has a wide range overlap with UV–vis absorption spectrum of DOX. Therefore, the fluorescence emission of UCNPs at 480 nm can be quenched by DOX. The UCNPs sensor has displayed a linear range of 0.05–20 μg/mL with a detection limit (LOD) of 0.005 μg/mL for DOX. Furthermore, the results of recovery for DOX in human serum samples is 98.68–104.20% and in accord with those obtained by HPLC and UV–vis.
研究领域:
生物医学工程
·
药物分析
·
纳米技术
✓ 已验证
DOI
高影响因子
CRYSTENGCOMM
·
2021
·Vol. 23
·期 21
·3892-3900
·
IF 3.545
Dandan Ju,Xiaoli Gao,Shouchao Zhang,Yang Li,Wenjing Cui,Yinhui Yang,Mingyan Luo,Shujing Liu
Temperature is one of the physical parameters that affects the emission luminescence properties of upconversion (UC) materials. Temperature-dependent emission color tuning and good temperature sensitivity in multifunctional β-NaYF4:Yb/Er@β-NaYF4:Yb/Tm microcrystals were achieved under 980 nm excitation. Based on the different emission bands (blue, green and red bands) and spectral sensitivities of the core–shell structured microcrystals to temperature, intense white-light emission was obtained at room temperature and a real-time naked-eye recognizable emission color change from white to the purplish-pink region was realized with the temperature increasing from 300 to 500 K. Furthermore, optical temperature sensing properties of the sample with good sensitivity (from 0.0034 to 0.0044 K−1) were investigated by using a fluorescence intensity ratio between 521 nm (2H11/2 → 4I15/2 transition) and 540 nm (4S3/2 → 4I15/2 transition) in the temperature range from 300 to 500 K. The results indicate that the sample has high sensitivity in a relatively wide range of temperature intervals, especially in the low temperature region. We believe that the multifunctional core–shell structured microcrystals exhibit promising applications in white-light emitting diodes, the anti-counterfeiting field, and temperature sensing.
研究领域:
材料科学
·
光学
✓ 已验证
产品关联
DOI
高影响因子
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