BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
·
2015
·Vol. 20
·期 5
·901-907
·
IF 1.113
Yang Jianbing,Ni Kefeng,Wei Dongzhi,Ren Yuhong
Ni 2+ -functionalized Fe 3 O 4 @polydopamine magnetic nanoparticles (Ni 2+ -PD-MNPs) were designed and synthesized by in situ coating of magnetic nanoparticles with polydopamine, followed by conjugation of Ni 2+ to the polydopamine film. The Ni 2+ -PD-MNPs were used to purify His-tagged red fluorescent protein (His-RFP) via affinity interaction between Ni 2+ and the His-tag. The results showed that the Ni 2+ -PD-MNPs had extraordinary selectivity for His-RFP purification. In addition, a Histagged transaminase (ω-transaminase BJ110) was selectively immobilized onto the Ni 2+ -PD-MNPs without purification, and the immobilized enzyme showed improved specific activity, as well as enhanced stability and reusability.
研究领域:
生物化学
·
纳米生物学
·
蛋白质工程
✓ 已验证
DOI
高影响因子
MICROPOROUS AND MESOPOROUS MATERIALS
·
2017
·
IF 3.615
Rong He,Zhihong Wang,Lei Tan,Yi Zhong,Weiming Li,Da Xing,Chaohai Wei,Youwen Tang
We developed two types of novel highly ordered Ni 2+ imprinted mesoporous organosilica materials (SBA-15 (NiIMS1) and MCM-41 (NiIMS2)) through co-condensation for selective adsorption of Ni 2+ from electroplating Ni 2+ effluents. FTIR and solid-state 13 C NMR spectra demonstrated the successful embedment of functionalized iminodiacetic acid and ethanediamine chelating groups in NiIMS1 and NiIMS2, respectively. X-ray photoelectron spectroscopy analysis confirmed that Ni 2+ was coordinated by N atom and carboxyl O atom of the functional groups. Furthermore, NiIMS1 and NiIMS2 displayed highly ordered periodic hexagonal P6mm meso-structure, large surface area, and highly specific recognition ability toward Ni 2+ . The adsorption experimental results proved that NiIMS1 was suitable for acidic wastewater treatment , whereas NiIMS2 was fit for alkaline wastewater. The saturated adsorption capacity of NiIMS2 was 20.8 mg/g at pH 6.0, and that of NiIMS1 reached up to 22.9 mg/g at pH 3.0. The values of selectivity coefficients of NiIMS1 and NiIMS2 for five competing metal ions ranged from 46.4 to 12.4 and 36.9 to 18.8, respectively. The adsorption efficiency of NiIMS1 was above 92.5% even after seven extraction-stripping cycles, while that for NiIMS2 was 90.9%. The real sample adsorption experiments demonstrated that these mesoporous organosilica materials have high potential for water treatment applications.
研究领域:
材料科学
·
环境科学
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
APPLIED SURFACE SCIENCE
·
2018
·
IF 4.439
Qingfeng Zhou,Yun Gong,Jianhua Lin
In the present work, Ni 2+ -doped (NH 4 ) 2 V 3 O 8 nanoflakes are in situ grown on Ni foam through a facile one-pot hydrothermal technique in a NH 4 VO 3 aqueous solution. The Ni 2+ -doped (NH 4 ) 2 V 3 O 8 @Ni foam composite material can be used as binder- and conductivity agent-free electrode in supercapacitor, it manifests a large specific capacitance of 465.5 F g −1 at a current density of 0.2 A g −1 and a superior rate capability of 317.5 F g −1 at 10 A g −1 , which is beneficial from its three-dimensional porous architecture cross-linked by the ultrathin Ni 2+ -doped (NH 4 ) 2 V 3 O 8 nanoflakes on Ni foam. Meanwhile, the Ni 2+ -doped (NH 4 ) 2 V 3 O 8 @Ni foam//Activated carbon asymmetric supercapacitor can deliver a maximum energy density of 20.1 W h kg −1 at a power density of 752.0 W kg −1 . Significantly, the Ni 2+ -doped (NH 4 ) 2 V 3 O 8 @Ni foam electrode possesses reversible electrochromic behavior, and it shows obvious visible light-driven photoresponse with much higher specific capacitance (645.3 F g −1 at 0.5 A g −1 ) under illumination (650 nm > λ > 350 nm, 100 mW cm −2 ), which is probably associated with the semiconducting characteristics of the spin-polarized (NH 4 ) 2 V 3 O 8 and the quantum confinement effect of the nanoflakes.
研究领域:
材料科学
·
能源存储与转换
✓ 已验证
产品关联
DOI
高影响因子
APPLIED CATALYSIS B-ENVIRONMENTAL
·
2018
·
IF 11.698
Qijie Mo,Wenbiao Zhang,Liuqing He,Xiang Yu,Qingsheng Gao
Exploring noble-metal-free electrocatalysts for hydrogen evolution reaction (HER) is the key issue in hydrogen economy blueprint. Herein, bimetallic Ni 2-x Co x P supported by N-doped carbon nanofibers (denoted as Ni 2-x Co x P/N-C NFs) are developed via electrospinning followed by pyrolysis under an inert flow. The space confinement by in-situ formed N-doped carbon matrix produces ultrafine Ni 2-x Co x P with abundant active-sites. More importantly, the engineering on Ni 2-x Co x P solid-solution-alloys can vary electronic configuration and consequently optimize hydrogen binding on electrocatalyst surface, accomplishing prominent HER activity in a wide pH range. The optimal Ni 2-x Co x P/N-C NFs afford low overpotentials ( η 10 ) of 100, 130 and 110 mV to reach a current density of -10 mA cm −2 in 0.5 M H 2 SO 4 , 1.0 KOH and 1.0 M PBS (phosphate buffer saline), respectively, performing among the best of noble-metal-free electrocatalysts. With a good functionality for oxygen evolution ( η 10 = 280 mV), such composite further delivers a high efficiency for overall water splitting, featuring a comparable cell voltage (1.56 V @ 10 mA cm −2 ) to a commercial IrO 2 /C − Pt/C couple, and remarkably better stability. Identifying electrocatalysis relying on solid-solution alloys, this work will inspire the exploration of cost-efficient electrocatalysts and the new understanding on catalytic mechanisms.
研究领域:
材料科学
·
电催化
·
能源化学
✓ 已验证
产品关联
DOI
高影响因子
IET Nanobiotechnology
·
2019
·Vol. 14
·期 1
·67-72
·
IF 1.925
Wenjing Wang,Fengzhen Zhou,Xiyao Cheng,Zhengding Su,Huiling Guo
To effective capture and universal enrichment of His-tagged protein, polyacrylic acid (PAA) brushes were used to encapsulate Fe 3 O 4 nanoparticles, connect NTA, and Ni 2+ to prepare magnetic beads. These materials provide many advantages, such as excellent stability, tuneable particle size, and a surface for further functionalisation with biomolecules. His-tagged green fluorescence protein (GFP) was separated efficiently, and the binding capacity of Fe 3 O 4 /MPS@PAA/NTA-Ni 2+ was 93.4 mg/g. Compared with High-Affinity Ni-NTA Resin and Ni-NTA Magnetic Agarose Beads, Fe 3 O 4 /MPS@PAA/NTA-Ni 2+ nanocomposites exhibited higher separation efficiency and binding capacity towards His-tagged GFP. Moreover, the selectivity and recyclability of them for the target proteins were maintained well after six cycles. This study would widen the application of PAA in constructing multifunctional nanocomposites for biomedical fields.
研究领域:
生物技术
·
蛋白质工程
·
纳米材料
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF WUHAN UNIVERSITY OF TECHNOLOGY-MATERIALS SCIENCE EDITION
·
2018
·Vol. 33
·期 3
·559-565
·
IF 0.524
Guo Huiling,Sun Honghao,Su Zhengding,Hu Shuchao,Wang Xiaotao
A facile approach has been developed to synthesize Fe 3 O 4 @PAM (polyacrylamide) nanoparticles (NPs) with carboxyl groups on the surfaces by copolymerization with acrylamide and acrylic acid in Fe 3 O 4 NPs aqueous suspension. Nitrilotriacetic acid (NTA) was conjugated to the magnetic NPs via well-known carboniimide chemistry using EDC and NHS. The Ni 2+ ions loaded on the surface of NPs provide abundant docking sites for immobilization of His-tagged green fluorescent proteins (His-tagged GFP). The high magnetic property of Fe 3 O 4 @PAM@NTA-Ni 2+ allows an easy separation of the NPs from solution under an external magnetic field, with high His-tagged protein binding capacity (42 μg protein/mg of NPs). The NPs can be recycled for at least four times without significant loss of binding capacity to proteins. These materials show great potential to separate His-tagged protein with low-cost purification at industrial scale.
研究领域:
材料科学
·
生物化学
·
生物技术
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
Laser & Photonics Reviews
·
2024
·Vol. 18
·期 11
·2400447
·
IF 9.8
Fangyi Zhao,Yuhe Shao,Quanlin Liu,Jiasong Zhong
Near-infrared (NIR) phosphor-converted light-emitting diode (pc-LED) light sources in the second NIR window (NIR-II, 1000–1700 nm) have sparked great interest for their emerging applications in non-destructive detection and medical diagnostics fields. However, the development of efficient NIR-II phosphors that can be excited by commercial blue LED chips remains a significant challenge, and thus impeding the applications of NIR pc-LED. Herein, a blue-light-excitable broadband NIR-II luminescence of Ni 2+ is achieved upon incorporating Cr 3+ and Ni 2+ into the Ca 3 Ga 2 Ge 3 O 12 (CGGO) host simultaneously. Through the energy transfer from Cr 3+ to Ni 2+ , CGGO:Cr 3+ ,Ni 2+ phosphor presents the improved NIR-II emission peaking at 1470 nm under 460 nm blue light excitation. The internal/external quantum efficiency values are significantly improved from 13.8%/3.3% to 24.6%/13.3% along with the enhanced luminescence thermal stability. Finally, a NIR pc-LED is fabricated by combining CGGO:Cr 3+ ,Ni 2+ phosphor with 450 nm blue chip, and its potential applications in non-destructive examination and biomedical imaging fields have been demonstrated. The strategy of Cr 3+ and Ni 2+ co-doping solves the problem that Ni 2+ luminescence cannot be effectively excited by blue light and provides the design insights to exploit more ultra-broadband NIR pc-LEDs based on blue LED chips.
研究领域:
材料科学
·
生物医学工程
·
光学成像
·
非侵入性检测
·
分子影像学
✓ 已验证
DOI
高影响因子
ENZYME AND MICROBIAL TECHNOLOGY
·
2018
·
IF 2.932
Juan Han,Yunfeng Cai,Yun Wang,Lei Gu,Chunmei Li,Yanli Mao,Wenli Zhang,Liang Ni
In this work, a novel Ni 2+ &AABOB bifunctional core–shell magnetic composite nanoparticles (MNPs), Fe 3 O 4 @p(AABOB-co-AIM-Ni 2+ ), were synthesized for the immobilization of horseradish peroxidase (HRP). Two kinds of interaction are simultaneously incorporated to improve the stability and reusability of immobilized HRP: one is the coordination between Ni 2+ and amide group in HRP, and the other is the interaction of the wulff-type boronic acid group with cis-diol-containing HRP which belongs to a type of glycoproteins. Accordingly, the proposed approach achieved high enrichment capacity (174.75 mg/g, protein/beads), and the activity of immobilized HRP still achieved 80% after reuse for 5 cycles. The degradation efficiency of phenol in the presence of immobilized HRP reached 93% within 10 min, which was significantly higher than that of free HRP only about 50% within 20 min. In addition, the immobilized HRP can be easily separated from the reaction solution by an external magnetic field.
研究领域:
生物化学
·
纳米技术
·
酶工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry C
·
2025
·Vol. 13
·期 18
·9382-9391
·
IF 5.1
Zaidong Chen,Yuefei Xiang,Xinghui Qin,Lei Zhong,Hong Liao,Shiwen Liu,Jiaqi Wang,Kezhi Zheng,Dejian Hou,Lei Zhou,Mingmei Wu
The challenges of low blue light absorption, reduced luminous efficiency, and low thermal stability are critical issues confronting near-infrared II (NIR-II) phosphors, which significantly hinder their applications in food testing, medical imaging, and various other fields. Herein, an energy transfer strategy was adopted to enhance blue light absorption by introducing Cr3+ into Lu2CaMg2Si3O12:Ni2+, thereby enhancing their luminescence properties, including quantum efficiency and thermal stability. Given the superior energy transfer efficiency of Cr3+ → Ni2+, the quantum efficiency of Lu2CaMg2Si3O12:Cr3+,Ni2+ was increased from 23.0% to 32.6%, and the thermal stability improved by 18%, as compared to Lu2CaMg2Si3O12:Ni2+. Furthermore, the Dexter's model was employed to systematically analyze the energy transfer mechanism of transition metal ions. In addition, due to the difference in the temperature response of Cr3+ and Ni2+, the thermometry performance of this phosphor was studied. Importantly, a pioneering method for food components detection based on the convolutional neural network model was proposed, demonstrating a 100% detection success rate. This research not only propels the development of novel blue-light-excitable NIR-II phosphors but also contributes to the advancement of intelligent lighting technologies.
研究领域:
材料科学
·
光学
·
人工智能
·
医学影像
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF SOL-GEL SCIENCE AND TECHNOLOGY
·
2014
·Vol. 71
·期 2
·380-384
·
IF 1.547
Li Binjie,Zhang Yongna,Cao Liuqin,Wu Yonghui,Zhao Yanbao
Silica (SiO 2 ) nanospheres (NSs) with immobilized metal ligands have been prepared for the affinity separation of proteins. First, SiO 2 NSs were prepared by controlled hydrolysis of tetraethoxysilane in a basic aqueous-ethanol solution. Then through reaction of iminodiacetic acid (IDA) with 3-glycidoxypropyltrimethoxysilane and immobilization of them onto the surfaces of above SiO 2 NSs, novel affinity adsorbents with IDA chelating groups were obtained. After chelating Ni 2+ ions, the SiO 2 –IDA–Ni 2+ NSs were applied to separate his-tagged proteins directly from the mixture of lysed cells. The SiO 2 –IDA–Ni 2+ NSs present negligible nonspecific protein adsorption and high protein binding ability (28.3 mg/g).
研究领域:
材料科学
·
生物化学
·
纳米技术
✓ 已验证
DOI
高影响因子
CERAMICS INTERNATIONAL
·
2021
·
IF 4.527
Deng-ming Wu,Xiao-pan Liu,Peng-zhao Gao,Luo-ting He,Jian-wei Li
Ni x Cu 0.2 Zn y Fe 1.98 O 4 ( x = 0.16, 0.26, 0.35, 0.43, 0.50; y = 0.8− x ) ferrites were prepared via solid-state reaction. The influence of Ni 2+ concentration on ferrites composition, microstructure, magnetic properties and DC-bias superposition characteristics was studied by XRD , SEM, XPS , and VSM. Then, the effect of magnetic properties on the DC-bias superposition characteristics was analyzed. The results showed that the ferrites sintered at 900 °C for 3 h were all in pure spinel phase with an average grain size of 7.5 μm and a relative density of about 5.15 g cm −3 . With the increase in Ni 2+ concentration, the saturation magnetization ( M s ) and coercivity ( H c ) of ferrites increased, but the magnetocrystalline anisotropy constant ( K 1 ) first increased and then decreased. And the M s , H c , and K 1 of the specimen with x = 0.43 were 63.62 emu·g −1 , 27.36 Oe, and 1813.17 Oe·emu·g −1 , respectively. In particular, the specimen of x = 0.50 achieved the best DC superposition characteristics, where the H 70% was about 220 A m −1 . The incremental permeability of the specimens decreased rapidly at a bias magnetic field <400 A m −1 . The variation mainly originated from the domain wall displacement, and the rate of decrease was influenced by both M s and K 1 . At a bias magnetic field ≥400 A m −1 , the incremental permeability of the specimens decreased slowly, mainly because the number of domain walls decreased until disappearing, and the specimens tended to become a single domain structure. And the rate of decrease of incremental permeability was mainly affected by K 1 .
研究领域:
材料科学
·
磁学
✓ 已验证
DOI
高影响因子
Nature Communications
·
2024
·Vol. 15
·期 1
·1-11
·
IF 14.7
Pei An,Wang Peng,Zhang Shiyi,Zhang Qinghua,Jiang Xiaoyi,Chen Zhaoxi,Zhou Weiwei,Qin Qizhen,Liu Renfeng,Du Ruian,Li Zhengjian,Qiu Yongcai,Yan Keyou,Gu Lin,Ye Jinyu,Waterhouse Geoffrey I. N.,Huang Wei-Hsiang,Chen Chi-Liang,Zhao Yun,Chen Guangxu
Challenges in direct catalytic oxidation of biomass-derived aldehyde and alcohol into acid with high activity and selectivity hinder the widespread biomass application. Herein, we demonstrate that a Pd/Ni(OH) 2 catalyst with abundant Ni 2+ -O-Pd interfaces allows electrooxidation of 5-hydroxymethylfurfural to 2, 5-furandicarboxylic acid with a selectivity near 100 % and 2, 5-furandicarboxylic acid yield of 97.3% at 0.6 volts (versus a reversible hydrogen electrode) in 1 M KOH electrolyte under ambient conditions. The rate-determining step of the intermediate oxidation of 5-hydroxymethyl-2-furancarboxylic acid is promoted by the increased OH species and low C–H activation energy barrier at Ni 2+ -O-Pd interfaces. Further, the Ni 2+ -O-Pd interfaces prevent the agglomeration of Pd nanoparticles during the reaction, greatly improving the stability of the catalyst. In this work, Pd/Ni(OH) 2 catalyst can achieve 100% 5-hydroxymethylfurfural conversion and >90% 2, 5-furandicarboxylic acid selectivity in a flow-cell and work stably over 200 h under a fixed cell voltage of 0.85 V.
研究领域:
电化学
·
催化
·
材料科学
·
可持续化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）