MICROCHEMICAL JOURNAL
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2025
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IF 5.1
Zhixue Yu,Yue He,Hui Wang,Dongxia Pan,Ruipeng Chen,Xiangfang Tang,Junhu Yao,Benhai Xiong
Ochratoxin A (OTA) is a highly toxic mycotoxin with nepHrotoxicity, hepatotoxicity, and carcinogenicity. It poses a long-term threat to the health of humans and animals. Although screen printed electrodes (SPE) with aptamer functionalization provide promising detection capabilities, their applications are limited by inherent weak electrochemical signals, prompting the need for signal amplification strategies. In this study, tetrahedral DNA clusters (TDCs) were prepared as aptamer probes for OTA, and an aptamer-based electrochemical sensing method for OTA was established for signal amplification. Multiple single-stranded DNA (ssDNA) were designed to self-assemble into tetrahedral DNA nanostructure (TDN) through base complementarity, with the OTA aptamer attached to one vertex. Additional ssDNA strands were further utilized to extend TDNs at the three remaining vertices, forming a DNA tetrahedral cluster. The method had a linear range of 1 pg/mL to 100 ng/mL (LOD = 1 pg/mL) for detecting OTA, with excellent specificity and satisfactory recovery rate in feed samples
研究领域:
生物传感器
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纳米技术
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生物化学
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分析化学
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分子生物学
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食品安全
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毒素检测
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DOI
高影响因子
MICROCHIMICA ACTA
·
2018
·Vol. 185
·期 5
·1-6
·
IF 5.705
Liu Renjie,Wu Hua,Lv Lei,Kang Xiaojiao,Cui Chengbi,Feng Jin,Guo Zhijun
This study describes an aptamer based assay for the mycotoxin ochratoxin A (OTA). The method is based on the use of an OTA-specific aptamer, exonuclease (Exo) III, SYBR Gold as a fluorescent probe, and a complementary strand that specifically combines with the aptamer. In the presence of OTA, the aptamer and OTA hybridize, thereby resulting in the formation of ssDNA, which is not digested by Exo III. Intense fluorescence is observed after addition of SYBR Gold (best measured at excitation/emission wavelengths of 495/540 nm). Fluorescence increases linearly with the log of the OTA concentration in the range from 8 to 1000 ng · mL −1 . The detection limit is 4.7 ng·mL −1 . The assay was applied to the determination of OTA in diluted [2%( v /v)] red wine, and recoveries and RSDs ranged between 93.5% and 113.8%, and between 3.2% and 5.7%, respectively.
In the presence of ochratoxin A (OTA), specific combinations of aptamer and OTA may occur and result in DNA double strands being untied, which avoids being digested by Exo III. Intense fluorescence is observed after SYBR Gold addition.
研究领域:
食品科学
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分子生物学
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DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
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2021
·
IF 10.588
Benchao Su,Zhong Zhang,Zhichang Sun,Zongwen Tang,Xiaoxia Xie,Qi Chen,Hongmei Cao,Xi Yu,Yang Xu,Xing Liu,Bruce D. Hammock
Ochratoxin A (OTA) contamination in food is a serious threat to public health. There is an urgent need for development of rapid and sensitive methods for OTA detection, to minimize consumer exposure to OTA. In this study, we constructed two OTA-specific fluonanobodies (FluoNbs), with a nanobody fused at the carboxyl-terminal (SGFP-Nb) or the amino-terminal (Nb-SGFP) of superfolder green fluorescence protein. SGFP-Nb, which displayed better fluorescence performance, was selected as the tracer for OTA, to develop a FluoNb-based nanosensor (FN-Nanosens) via the fluorescence resonance energy transfer, where the SGFP-Nb served as the donor and the chemical conjugates of OTA-quantum dots served as the acceptor. After optimization, FN-Nanosens showed a limit of detection of 5 pg/mL, with a linear detection range of 5–5000 pg/mL. FN-Nanosens was found to be highly selective for OTA and showed good accuracy and repeatability in recovery experiments using cereals with various complex matrix environments. Moreover, the contents of OTA in real samples measured using FN-Nanosens correlated well with those from the liquid chromatography with tandem mass spectrometry . Therefore, this work illustrated that the FluoNb is an ideal immunosensing tool and that FN-Nanosens is reliable for rapid detection of OTA in cereals with ultrahigh sensitivity.
研究领域:
食品安全
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生物传感
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分子生物学
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DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
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2017
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IF 7.78
Shaoliang Dai,Shijia Wu,Nuo Duan,Jian Chen,Zhigao Zheng,Zhouping Wang
We developed an ultrasensitive luminescence resonance energy transfer (LRET) aptasensor for Ochratoxin A (OTA) detection, using core/shell upconversion nanoparticles (CS-UCNPs) as luminophores. The OTA aptamer was tagged to CS-UCNPs as energy donor and graphene oxide (GO) acted as energy acceptor. The π-π stacking interaction between the aptamer and GO brought CS-UCNPs and GO in close proximity hence initiated the LRET process resulting in quenching of CS-UCNPs luminescence. A linear calibration was obtained between the luminescence intensity and the logarithm of OTA concentration in the range from 0.001 ng mL −1 to 250 ng mL −1 , with a detection limit of 0.001 ng mL −1 . The aptasensor showed good specificity towards OTA in beer samples. The ultrahigh sensitivity and pronounced robustness in beer sample matrix suggested promising prospect of the aptasensor inpractical applications.
研究领域:
分析化学
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食品安全
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纳米技术
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DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2019
·
IF 2.931
Yanhua He,Youwei Yu,Xiaoye Wen,Yan Shi,Jianhu Wu,Zhengping Guan,Meilin Cui,Chunling Xiao
A sensitive, efficient and quencher-free fluorescence aptasensor to detect Ochratoxin A (OTA) based on aptamer , 2-aminopurine (2AP) labeled Oligonucleotide sequence, as well as exonuclease I (Exo I) activity was developed. In which the aptamer specific to OTA was modified into a hairpin structure, and 8 bases at the 3′ ends are exposed (H); also, 2AP is embedded in the oligonucleotide complementary to the 8 bases (2AP-probe).The detection principle based on 2AP-probe could be bonded to its complementary sequence and quenches the fluorescence of 2AP; The aptamer has a stronger affinity for the target than its complementary sequence; Exo I can dissociate single-stranded DNA and has little effect on double-stranded DNA as well as folded DNA. In the absence of OTA, the fluorescence of 2AP is quenched due to the complementary pairing of H and 2AP-probe; in the presence of OTA, H selective binding target is detached from 2AP-probe, and the fluorescence of 2AP is slightly restored. Moreover, when the Exo I is added to the detection system, 2AP-probe is dissociated by the Exo I to release the free 2AP, and the fluorescence of the system is further enhanced thereby realizing the detection of OTA. The detection limit of the aptasensor was low as 0.03 nM with a linear range of 0.5–100 nM. Moreover, the aptasensor has good selectivity and practicability and also has good potential in realizing the detection of toxic and harmful substances in food complex matrices.
研究领域:
食品科学
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分子生物学
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DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
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2014
·
IF 4.517
Chengke Wang,Xiaoya Dong,Qian Liu,Kun Wang
The combination of high selectivity of aptamer with the peroxidase-mimicking property of DNAzyme has presented considerable opportunities for designing colorimetric aptasensor for detection of ochratoxin A (OTA). The activities of both aptamer (as biorecognition element) and DNAzyme (as signal amplification element) are blocked via base pairing in the hairpin structure. Hybridization chain reaction (HCR) between two hairpin DNAs was employed to further improve the sensitivity of this method. The presence of OTA triggers the opening of the hairpin structure and the beginning of HCR, which results in the release of many DNAzyme, and generates enhanced colorimetric signals, which is correlated to the amounts of OTA with linear range between 0.01 to 0.32 nM, and the limit of detection is 0.01 nM under optimal conditions. OTA in yellow rice wine and wheat flour samples was also detected using this method. We demonstrate that a new colorimetric method for the detection of OTA has been established, which is simple, easy to conduct, label-free, sensitive, high throughput, and cost-saving.
研究领域:
食品科学
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分析化学
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生物技术
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DOI
PubMed
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
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2019
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IF 4.527
Zhichang Sun,Xuerou Wang,Zongwen Tang,Qi Chen,Xing Liu
A biotin-streptavidin-amplified enzyme-linked immunosorbent assay using a biotinylated nanobody (BA-Nb ELISA) was developed to detect ochratoxin A (OTA) in cereal. The limit of detection (LOD) of the BA-Nb ELISA, which equals to 10% maximal inhibitory concentration, was 0.011 ng/mL for OTA in buffer, and the sensitivity was approximately improved by one order of magnitude compared with the traditional Nb ELISA (LOD = 0.112 ng/mL). Under optimal conditions, the developed assay could be accomplished in 40 min with maximal inhibitory concentration of 0.138 ng/mL and the linear detection range of 0.034–0.460 ng/mL. The average recovery rate of the BA-Nb ELISA ranged from 92.8% to 114%, and the relative standard deviation was in the range of 2.04–9.85%. The developed BA-Nb ELISA was validated by liquid chromatography-tandem mass spectrometry (LC-MS/MS), and the results indicated the reliability of BA-Nb ELISA for the detection of OTA in cereal.
研究领域:
分析化学
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食品科学
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毒理学
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DOI
PubMed
高影响因子
FOOD AND AGRICULTURAL IMMUNOLOGY
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2020
·
IF 0
Lei Ma,Xi Qiu,Yaqi Li,Sheng Tang,Wei Shen,Changrui Xing,Dezhao Kong,Jianguo Sheng
Ochratoxin A (OTA) is a highly toxic potential contaminant of most agricultural products, and OTA is difficult to remove in general food production processes. In this study, we immobilized polyvinyl pyrrolidone-coated carboxypeptidase A (CPA) into zeolitic imidazolate framework material to improve the ability for OTA degradation. In this method, carboxypeptidase A was dispersed well in the material and maintained its stability and ability to degrade OTA, under complex matrix conditions with better repeated use performance than free carboxypeptidase A. The substrate conversion rate of CPA increased 3 times after immobilization. After repeated use of 10 cycles, the composite enzyme still retained 71.37% of its initial activity. The degradation rate of OTA was 30.69% higher than that of free CPA. This method may play an important role in the degradation of mycotoxins.
研究领域:
食品科学
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生物化学
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产品关联
DOI
Frontiers in Chemistry
·
2022
·
IF 5.545
Xiao Ni,Yuyan Zhang,Chuhan Xue,Xiaojun Chen
Ochratoxin A (OTA) is a harmful mycotoxin, which is mainly secreted by Penicillium and Aspergillus species. In this work, an electrochemical aptasensor is presented for OTA detection based on Au nanoparticles (AuNPs) modified zeolite imidazolate frameworks (ZIFs) ZIF-8 platform and duplex-specific nuclease (DSN) triggered hybridization chain reaction (HCR) signal amplification. G-quadruplex-hemin assembled HCR nanowire acted as a nicotinamide adenine dinucleotide (NADH) oxidase and an HRP-mimicking DNAzyme. Besides, thionine (Thi) was enriched as a redox probe for signal amplification in this pseudobienzyme electrocatalytic system. Under the optimal conditions, the analytical response ranged from 1 to 10 7 fg ml −1 with a detection limit of 0.247 fg ml −1 . Furthermore, the aptasensor was proven to be applied in real wheat samples with a recovery between 96.8 and 104.2%, illustrating the potential prospects in practical detection.
研究领域:
分析化学
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毒理学
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食品科学
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纳米技术
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电化学
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产品关联
DOI
PubMed
高影响因子
Foods
·
2022
·Vol. 11
·期 21
·3407
·
IF 5.561
Hao Wang,Lu Chen,Min Li,Yongxin She,Chao Zhu,Mengmeng Yan
Avoiding interference and realizing the precise detection of mycotoxins in complex food samples is still an urgent problem for surface-enhanced Raman spectroscopy (SERS) analysis technology. Herein, a highly sensitive and specific aptasensor was developed for the anti-interference detection of Ochratoxin A (OTA). In this aptasensor, 4-[(Trimethylsilyl) ethynyl] aniline was employed as an anti-interference Raman reporter to prove a sharp Raman peak (1998 cm−1) in silent region, which could avoid the interference of food bio-molecules in 600–1800 cm−1. 4-TEAE and OTA-aptamer were assembled on Au NPs to serve as anti-interference SERS probes. Meanwhile, Fe3O4NPs, linked with complementary aptamer (cApts), were applied as capture probes. The specific binding of OTA to aptamer hindered the complementary binding of aptamer and cApt, which inhibited the binding of SERS probes and capture probes. Hence, the Raman responses at 1998 cm−1were negatively correlated with the OTA level. Under the optimum condition, the aptasensor presented a linear response for OTA detection in the range of 0.1–40 nM, with low detection limits of 30 pM. In addition, the aptasensor was successfully applied to quantify OTA in soybean, grape and milk samples. Accordingly, this anti-interference aptasensor could perform specific, sensitive and precise detection of OTA in real samples, and proved a reliable reference strategy for other small-molecules detection in food samples.Keywords:SERS;4-TEAE;aptamer;silent region;OTA;anti-interference
研究领域:
分析化学
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食品科学
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毒理学
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DOI
PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2022
·
IF 5.518
Lei Jiang,Yaning Han,Youxun Li,Zhuang Li,Shuyuan Zhang,Xiudong Zhu,Zhibin Liu,Yuxue Chen,Susana Fernandez-Garcia,Yongjiao Tang,Xiaowei Chen
The ubiquitous presence of ochratoxin A (OTA), a toxic food contaminant, requires development of fast and sensitive detection tools. However, it is difficult to find a method that can address high sensitivity and wide detection range simultaneously. In this work, a sensing platform composed of praseodymia nanorods (Pr 6 O 11 -NRs) and aptamers for OTA is established. Under the dual-readout modes, this platform provides a highly sensitive detection of OTA with extra-wide range, over three orders of magnitude with limit of detection (LOD) of 0.8 ng/mL. Both fluorometric and colorimetric signals were obtained because of the excellent fluorescent quenching and oxidase-like activity of Pr 6 O 11 -NRs. This probe enables a detection range of OTA which covers the maximum acceptable levels of most foods. Real samples were tested with this method, demonstrating high sensitivity and good recoveries. This sensing strategy has great potential for an expanded application in point-of-care detection of other toxins via portable devices.
研究领域:
食品安全
·
分析化学
·
纳米技术
·
毒理学
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产品关联
DOI
高影响因子
PHYTOMEDICINE
·
2023
·
IF 7.9
Xin Liu,Chunli Yan,Chunxiao Chang,Fansong Meng,Wenjie Shen,Song Wang,Yi Zhang
Background Mycotoxins, such as aflatoxin and ochratoxin A (OTA), are found at measurable levels in many staple foods; the health implications of long-term exposure of such toxins are poorly understood. Increasing evidence has confirmed the important role of OTA in upregulation of oxidative stress- and inflammatory response-induced tissue injury. However, it remains unknown whether ochratoxin A can promote chronic colitis and its associated colon cancer (CRC) development, and potential molecular mechanism. Additionally, RING finger-interacting protein with C kinase (RINCK) is a ubiquitin ligase and mediates immune response. Unfortunately, the potential molecular function of RINCK on regulation of colitis is still largely unknown. Purpose This study aims to provide mechanistic evidence that the role of RINCK in colitis and early colorectal cancer progression in response to OTA treatment via targeting nuclear factor erythroid 2-related factor 2 (NRF2). Methods The Cancer Genome Atlas (TCGA) database, GEO database, human subjects with CC phenotype and CC cell lines were used in this work. Pathological links between OTA, RINCK and treatment of CC are revealed through comprehensive means such as biological information analysis, clinical experiments, RNA-seq, and verification experiments. Results In this study, under oxidative stress in setting of colitis, we first identified RINCK as a key regulatory factor and a novel endogenous suppressor of nuclear factor erythroid 2-related factor 2 (NRF2), and we also confirm that RINCK is a NRF2 partner protein that catalyses its ubiquitination and degradation in intestinal epithelial cells (IECs). Notably, in vivo study, pathological phenotypes triggered by OTA pretreatment, accompanied by post-treatment of dextran sulfate sodium (DSS)-induced colitis was significantly mitigated by IEC-specific deficiency of Rinck , IEC- Rinck (KO) and adenovirus-associated virus (AAV)-triggered suppression of Rinck in rodent model, and lentivirus (LV)-mediated downregulation of Rinck (LV-sh Rinck ) in rabbit model, as determined by decreased endogenous reactive oxygen species (ROS) production, pro-inflammatory cytokines contents, improved body weights, reduced survival rates, restored colon length, assuasive DAI and histological scores. Inversely, transgenic mice by IEC-specific Rinck overexpression, IEC- Rinck (OE) accelerated colitis in acute or chronic colitis rodent models and in vitro experiments. Moreover, we found that OTA pretreatment-promoted azoxymethane (AOM)/DSS-induced colitis-associated early colorectal cancer (CRC) was also dramatically reduced by IEC- Rinck (KO), indicated by the decreased tumor number and corresponding KI-67 levels. Clinical samples analysis revealed that RINCK levels were greatly increased in tumor tissues of patients with CRC phenotypes. In parallel, RINCK deletion remarkably retarded the proliferation of colon cancer and tumor growth in vitro and in vivo , respectively. Mechanistically, in response to onset of colitis, RINCK directly interacts with NRF2 and promotes ubiquitin-proteasome degradation via increasing K48-linkage ubiquitin chain, thus leads in suppression of NRF2 nuclear translocation and its downstream cascade inactivation, which retards antioxidant defense. Conclusion The findings suggested that oral sub-chronic exposure of OTA significantly facilitates DSS-induced colitis and colitis-associated CRC development. These results further elucidated the potential role of RINCK in colitis progression by mediating NRF2 degradation, and could be considered as a therapeutic target for the treatment of such disease.
研究领域:
毒理学
·
免疫学
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癌症生物学
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分子生物学
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胃肠病学
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产品关联
DOI
PubMed
高影响因子
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