ANALYTICAL CHEMISTRY
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2022
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IF 8.008
Jinzheng Zhang,Shuailong Zhou,Shiyi Tan,Kangyan Yi,Mengya Jin,Qian Shi,Fen Wu,Nannan Liu
Pore structure-based analytical techniques have great potential applications for the detection of biological molecules. However, the sophistication of traditional pore sensors is restricted in their applicability of analytical chemistry due to a lack of effective carrier probes. Here, we used porous coordination network-224 (PCN-224) composite probes in conjunction with a glass nanopipette (GN) as a sensing platform. The sensor exhibits a good fluorescence signal and a change in GN’s ionic current at the same time. Due to the volume exclusion mechanism coming from PCN-224, the detection limit of target DNA reaches 10 fM in a GN with a diameter of up to ca. 260 nm, outperforming a simple probe. The structure of the composite probe is optimized by the probe’s pairing efficiency. Furthermore, the sensor can also discriminate between 1-, 3-, and 5-mismatch DNA sequences and capture the target DNA from a complex mixture. Based on the GN platform, a series of techniques for detecting biomolecules are expected to emerge because of its simplicity, robustness, and universality by incorporating advanced nanoprobes.
研究领域:
分析化学
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生物传感器
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纳米技术
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分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INORGANIC CHEMISTRY COMMUNICATIONS
·
2025
·
IF 5.4
Yapu Yang,Yalin Chang,Yueshang Wang,Yinuo Gai,Jun Ma,Pengfei Shi
Phototherapy is rapidly evolving in the treatment of bacterial infections to counter the dangers of bacterial evolution and the development of bacterial resistance due to the misuse of antibiotics. Herein, we report a nano-antimicrobial platform (PCN-224-IrO 2 -HA), constructed by iridium oxide nanoparticles and metal-organic framework PCN-224. PCN-224-IrO 2 -HA is capable of the photodynamic therapy (PDT), which can induce bacterial death by converting surrounding O 2 to reactive oxygen species under laser irradiation. Meanwhile, IrO 2 can catalyse the decomposition of H 2 O 2 to generate O 2 and enhance PDT. Meanwhile, IrO 2 can rapidly convert light energy into thermal energy under laser irradiation, so that it is able to kill bacteria by photothermal therapy (PTT). Photodynamic therapy synergised with photothermal therapy significantly inhibit the growth of bacteria, providing a new idea for phototherapy to treat bacterial infections.
研究领域:
纳米医学
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抗菌治疗
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光疗
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材料科学
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传染病
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2025
·
IF 6.1
Lin Chai,Haoyu Chen,Xing Yang,Jing Liu,Qiquan Yang,Shu Huang,Min Tang,Xiaohua Zhu,Haitao Li,Youyu Zhang,Meiling Liu
Ratiometric fluorescent nanoplatforms with self-calibrating capabilities offer enhanced detection accuracy by minimizing environmental interference. However, challenges such as signal cross-interference and nanomaterial instability persist in complex biological systems. To address these issues, we developed a glutathione (GSH)-responsive ratiometric fluorescent nanoprobe by integrating a porphyrin-based metal-organic framework (PCN-224) with MnO 2 nanosheets (NSs). The PCN-224 serves as both a nanocarrier and luminescent moiety, while MnO 2 acts as oxidase (OXD) mimetic nanozyme and the reactive site for GSH. The ratiometric detection mechanism relies on the intrinsic fluorescence of PCN-224 (emission at 655 nm) and the OXD-like activity of PCN-224@MnO 2 , which catalyzes the oxidation of o -phenylenediamine (OPD) to generate fluorescent 2,3-diaminophenazine (DAP, emission at 570 nm). Upon GSH exposure, the redox reaction between GSH and MnO 2 produces Mn 2+ , quenching the OXD-like activity and diminishing the DAP fluorescence, while the PCN-224 fluorescence recovers due to MnO 2 decomposition. The nanoplatform exhibits a wide detection range spanning from 0.1 to 60 μM with a low detection limit of 15 nM and high selectivity. Furthermore, it enables accurate GSH quantification in complex biological matrices, including human serum and cell lysates, and facilitates real-time monitoring of endogenous and exogenous GSH in living cells via fluorescence imaging. This study not only presents sensitive and reliable platform for GSH detection, but also overcomes key limitations in ratiometric sensing, such as signal interference and nanomaterial instability, by utilizing a stimuli-responsive fluorescent nanoprobe design.
研究领域:
分析化学
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纳米技术
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生物化学
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生物传感器
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荧光成像
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材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
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2024
·
IF 12.2
Wei Chen,Xiayu Peng,Lichao Kang,Shengnan Dong,Jian Zhang,Yunfeng Zhao,Fengxia Sun
The development of a versatile platform for bacterial assay and elimination is urgently needed due to the danger that bacteria pose to human life. Here, we synthesized a trimetallic deposition and horseradish peroxidase (HRP)-embedded porous coordination network-224 hybrid nanozymes (PCN-224 @AuPdPt@HRP) with outstanding peroxidase activity and fluorescence quenching ability. On this basis, we designed a dual recognition strategy-driven colorimetric-fluorescence dual-mode detection platform using Listeria monocytogenes as a pattern analyte. The platform consisted of an aptamer-modified PCN-224 @AuPdPt@HRP (PCN-224 @AuPdPt@HRP@Aptamer) specifically recognizing Listeria monocytogenes and vancomycin-coated 96-well plates. In the presence of vancomycin, which has the ability to recognize and inactivate gram-positive bacteria, the significant peroxidase activity of PCN-224 @AuPdPt@HRP@Aptamer in the precipitate was able to catalyze the color change of the substrate by H 2 O 2 . Meanwhile, the residual PCN-224 @AuPdPt@HRP@Aptamer in the supernatant was able to change the fluorescence of fluorescein-labeled deoxyribonucleic acid (FAM-DNA). In summary, this paper presents a multifunctional platform capable of detecting and eliminating residual bacteria in real environments. This strategy is expected to facilitate the development of multifunctional biosensors based on metal–organic framework probes and also provide environmental health.
研究领域:
生物技术
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分析化学
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微生物学
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材料科学
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环境健康
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nanomedicine-Nanotechnology Biology and Medicine
·
2023
·
IF 5.4
Jihua Tian,Jing Wang,Huanyu Xu,Bocheng Zou,Weihao Chen,Yulong Liu,Jingshu Chen,Ruiping Zhang
Rapamycin has great potential in the antitumor application, but its therapeutic effect is seriously affected by poor water solubility, targeting ability, and low bioavailability. Here, we constructed a novel composite nanomaterial with PCN-224 as a drug carrier and loaded rapamycin, named [email protected] @HA. The nanoplate not only improves targeting, but also synergizes rapamycin with PCN-224 to effectively promote tumor cell apoptosis , which subsequently causes immunogenic cell death (ICD), and shows strong therapeutic effect in 4T1 breast cancer model. The treatment effect depends on three main points:(i)Proapoptotic effect of rapamycin on tumor cells;(ii)ROS production by PCN-224-mediated photodynamic therapy;(iii)ICD induced DC maturation, increased immune response and promoted T cell proliferation and differentiation. This nanoplate offers potential antitumor efficacy in combination with chemotherapy, photodynamic therapy, and immunotherapy .
研究领域:
癌症研究
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纳米技术
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免疫疗法
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药学
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生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Advanced Healthcare Materials
·
2024
·
IF 10
Wei Zhang,Yijun Ran,Mi Yang,Yaqin Hu,Zhigang Wang,Yang Cao,Haitao Ran
Amplifying oxidative stress to disrupt intracellular redox homeostasis can accelerate tumor cell death. In this work, an oxidative stress amplifier (PP@T) is prepared for enhanced tumor oxidation therapy to reduce tumor growth and metastases. The nano-amplifier has been successfully constructed by embedding MTH1 inhibitor (TH588) in the PDA-coated porphyrin metal–organic framework PCN-224. The controllable-released TH588 is demonstrated from pores can hinder MTH1-mediated damage-repairing process by preventing the hydrolysis of 8-oxo-dG, thereby amplifying oxidative stress and exacerbating the oxidative DNA damage induced by the sonodynamic therapy of PP@T under ultrasound irradiation. Furthermore, PP@T can effectively induce immunogenic cell death to trigger systemic anti-tumor immune response. When administered in combination with immune checkpoint blockade, PP@T not only impedes the progression of the primary tumor but also achieves obvious antimetastasis in breast cancer murine models, including orthotopic and artificial whole-body metastasis models. Furthermore, the nanoplatform also provides photoacoustic imaging for in vivo treatment guidance. In conclusion, by amplifying oxidative stress and reactive oxygen species sensitized immunotherapy, this image-guided nanosystem shows potential for highly specific, effective combined therapy against tumor cells with negligible side-effects to normal cells which will provide a new insight for precise tumor treatment.
研究领域:
肿瘤学
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生物医学工程
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免疫治疗
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纳米技术
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癌症治疗
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nanoscale Horizons
·
2024
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IF 8
Yue Chen,Tong Ding,Zhengzheng Qian,Zerui Ma,Liming Zhou,Zhiling Li,Runkai Lv,Yinghui Xu,Yingjie Xu,Linhui Hao,Chen Zhu,Xikuang Yao,Wenying Yu,Wenpei Fan
Sonodynamic therapy (SDT) is gaining popularity in cancer treatment due to its superior controllability and high tissue permeability. Nonetheless, the efficacy of SDT is severely diminished by the transient generation of limited reactive oxygen species (ROS). Herein, we introduce an acid-activated nanosonosensitizer, CaO2@PCN, by the controllable coating of porphyrinic metal-organic frameworks (PCN-224) on CaO2 to induce cascaded oxidative stress in tumors. The PCN-224 doping could generate ROS during SDT to induce intracellular oxidative stress and abnormal calcium channels. Meanwhile, the ultrasound also promotes extracellular calcium influx. In addition, the CaO2@PCN will sequentially degrade in the tumor cell lysosomes, releasing Ca2+ and H2O2 to induce further abnormal calcium channels and elevate the levels of Ca2+. Insufficient catalase (CAT) in tumor cells promotes intracellular calcium overload, which can induce persistent ROS generation and mitochondrial dysfunction through ion interference therapy (IIT). More importantly, PCN-224 also protects CaO2 against significant degradation under neutral conditions. Hence, the well-designed CaO2@PCN produces synergistic SDT/IIT effects and persistent ROS against cancer. More notably, the acidity-responsive biodegradability endows CaO2@PCN with excellent biosafety and promising clinical potential.
研究领域:
癌症治疗
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纳米技术
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生物医学工程
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材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CATALYSIS TODAY
·
2021
·
IF 6.766
Liangliang Zhang,Yang Feng,Huihui He,Yang Liu,Jiena Weng,Peng Zhang,Wei Huang
In this article, we report a series of hexanuclear rare-earth MOFs based on Ce (III) and 5,10,15,20-tetrakis(4-carboxyphenyl)porphyrin, namely Ce-PCN-224, with a new zln topology through linker induce strategy. Ce-PCN-224 exhibits permanent porosity, high water and thermal stabilities, and the ability to adsorb dye molecules in the aqueous environment. Moreover, after post-metallation with Fe, Co, and Ni, Ce-PCN-224(Fe) and Ce-PCN-224(Co) were proved to be efficient heterogeneous catalysts for the cycloaddition of CO 2 with epoxides, attributed to the combination of multiple Lewis acidic sites.
研究领域:
材料科学
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化学工程
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环境科学
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催化
✓ 已验证
产品关联
DOI
高影响因子
Nature Communications
·
2024
·Vol. 15
·期 1
·1-18
·
IF 14.7
Su Zheng,Xu Dongdong,Hu Xianli,Zhu Wanbo,Kong Lingtong,Qian Zhengzheng,Mei Jiawei,Ma Ruixiang,Shang Xifu,Fan Wenpei,Zhu Chen
Pathogen-host competition for manganese and intricate immunostimulatory pathways severely attenuates the efficacy of antibacterial immunotherapy against biofilm infections associated with orthopaedic implants. Herein, we introduce a spatiotemporal sono-metalloimmunotherapy (SMIT) strategy aimed at efficient biofilm ablation by custom design of ingenious biomimetic metal-organic framework (PCN-224)-coated MnO 2 -hydrangea nanoparticles (MnPM) as a metalloantibiotic. Upon reaching the acidic H 2 O 2 -enriched biofilm microenvironment, MnPM can convert abundant H 2 O 2 into oxygen, which is conducive to significantly enhancing the efficacy of ultrasound (US)-triggered sonodynamic therapy (SDT), thereby exposing bacteria-associated antigens (BAAs). Moreover, MnPM disrupts bacterial homeostasis, further killing more bacteria. Then, the Mn ions released from the degraded MnO 2 can recharge immune cells to enhance the cGAS-STING signaling pathway sensing of BAAs, further boosting the immune response and suppressing biofilm growth via biofilm-specific T cell responses. Following US withdrawal, the sustained oxygenation promotes the survival and migration of fibroblasts, stimulates the expression of angiogenic growth factors and angiogenesis, and neutralizes excessive inflammation. Our findings highlight that MnPM may act as an immune costimulatory metalloantibiotic to regulate the cGAS-STING signaling pathway, presenting a promising alternative to antibiotics for orthopaedic biofilm infection treatment and pro-tissue repair.
研究领域:
生物医学工程
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材料科学
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免疫学
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骨科
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传染病
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF RARE EARTHS
·
2025
·
IF 7.2
Ming Qian,Jiarui Du,Qichuan Jiang,Wei Tao,Jiayi Zhang,Liang Gong
Early diagnosis and treatment of cancer are key problems for effective cancer therapy. Therefore, the development of multifunctional materials that can integrate imaging and therapeutic functions is of great importance. Lanthanide-doped nanoparticles@metal-organic frameworks (Ln-NPs@MOFs) offer an unprecedented opportunity to address this challenge, by combining the imaging capabilities of rare earth-doped nanoparticles with the photosensitizing properties of MOFs for photodynamic therapy (PDT). Here, we report a near-infrared (NIR) light-responsive nanoparticle based on a zirconium-porphyrin metal-organic framework (PCN-224) for synergistic photothermal and photodynamic therapy. Tetra(4-carboxyphenyl)porphyrin (TCPP) that constitutes the MOF framework serves as a natural photosensitizer, enabling efficient photodynamic therapy through Förster resonance energy transfer (FRET). The dye indocyanine green (ICG) was loaded into the MOF pores to sensitize the Ln nanoparticles, enhancing the upconversion fluorescence by 41 times and the downconversion fluorescence by 5 times, which is beneficial to the biological efficacy for imaging diagnosis. Meanwhile, ICG, a traditional photothermal agent, also imparts photothermal therapy capabilities to the system. Furthermore, the encapsulation of cancer cell membranes not only enhances biocompatibility but also improves the material's targeting towards cancer cells, thereby greatly enhancing the therapeutic effect. This dual-functional material provides a promising platform for the integration of cancer diagnosis and therapy.
研究领域:
生物医学工程
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纳米医学
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癌症诊疗一体化
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光学成像
·
光疗
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）