RSC Advances
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2021
·Vol. 11
·期 32
·19450-19460
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IF 3.361
Zhao Wang,Jingjing Sun,Mingrui Li,Ting Luo,Yulin Shen,Amin Cao,Ruilong Sheng
Using natural-based lipids to construct biocompatible, controllable and efficient nanocarriers and elucidating their structure–function relationships, was regarded as an important area for creating sustainable biomaterials. Herein, we utilized two natural steroids: cholesterol and diosgenin (bearing different hydrophobic tails) as the building blocks, to synthesize a series of natural steroid-based cationic random copolymers PMA6Chol-r-PDMAEMA and PMA6Dios-r-PDMAEMA via RAFT polymerization. The results demonstrated that the steroid-r-PDMAEMA copolymers could efficiently bind pDNA (N/P < 3.0) and then form near-spherical shape (142–449 nm) and positively-charged (+11.5 to +19.6 mV) nanoparticles. The in vitro cytotoxicity and gene transfection efficiency greatly depend on the steroid hydrophobic tail structures and steroid/PDMAEMA block ratios. Optimum transfection efficiency of the (Chol-P1/pDNA and Dios-P3/pDNA) nanoplexes could reach to 18.1–31.2% of the PEI-25K/pDNA complex. Moreover, all of the steroid-r-PDMAEMA/Cy3-pDNA nanoplexes have an obvious “lysosome localization” effect, indicating the steroid structures do not remarkably influence the intracellular localization behaviors of these nanoplexes.
研究领域:
生物技术
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药学
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材料科学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
POLYMER
·
2022
·
IF 4.432
Jing Yan,Jianghao Yao,Yinghui Hu,Diping Huang,Dongdong Yao,Fangzhou Wu,Qiuyu Zhang,Yi Yan
To enrich the building block and function as well as the preparation methodology of metallopolymer system, functioned inorganic polyoxometalate clusters were introduced by using their intrinsic acidity induced multiple interactions with triblock copolymers . In the designed triblock copolymer PEG- b -PDMAEMA- b -PTEPM, the POMs clusters can be immobilized through in-situ protonation of the PDMAEMA block induced electrostatic interaction and the self-gelation of the PTEPM block, while the PEG block can improve the water solubility and dispersion of the final polyoxometalate-based hybrid catalyst (PHC). The selective oxidation activity of PHC is comparable with the homogeneous POMs catalyst as demonstrated by the model reaction of the selective oxidation of methyl phenyl sulfide (MPS) to methyl phenyl sulfoxide (MPSO). By optimizing the ratio between PHC and oxidant H 2 O 2 , the selectivity for MPSO can achieve as high as 99% with conversion above 95%. Furthermore, the multiple interactions between POMs and the polymer substrate also enhance the stability of PHC and enable the recycling of PHC. Therefore, the methodology of in-situ protonation and self-gelation by the acidity of POMs provides a facile and general strategy to prepare robust POMs-based metallopolymers, paving a new avenue towards cluster-based materials and devices.
研究领域:
材料科学
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催化化学
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产品关联
DOI
高影响因子
DRUG DEVELOPMENT AND INDUSTRIAL PHARMACY
·
2024
·
IF 2.4
Xingbo Wang,Hong Huang,Wenxiu Xu,Yanling Gong,Songbo Shi,Xu Wan,Li Pengbiao
Introduction Triple negative breast cancer (TNBC) is characterized with higher malignancy and mortality and is prone to distant metastasis, among which bone is the most common site. It’s urgent to explore new strategies for treatment of TNBC and its bone metastases.Methods A tumor environment responsive vector, poly-(dimethylaminoethyl methacrylate)-SS-poly(ethylene glycol)-SS-poly-(dimethylaminoethyl methacrylate) (PDMAEMA-SS-PEG-SS-PDMAEMA), was constructed to co-delivery transforming growth factor-β1 (TGF-β1) siRNA and forkhead box M1 (FOXM1) siRNA in MDA-MB-231 cells. The preparation, characterization, in vitro release, stability, and transfection efficiency of nanoparticles were measured. Cell viability, migration and invasion of MDA-MB-231 cells were determined. Cell chemotactic migration and cell heterogeneity adhesion of MDA-MB-231 cells to the human osteoblast-like cell line MG-63 were determined.Results PDMAEMA-SS-PEG-SS-PDMAEMA self assembled with siRNA at N/P of 15:1 into nanoparticles with particle size of 122 nm. In vitro release exhibited redox and pH sensitivity, and the nanoparticles protected siRNA from degradation by RNase and serum protein, remaining stable at 4 °C with similar transfection efficiency with lipo2000. Nanoparticles co-loaded with TGF-β1 siRNA and FOXM1 siRNA inhibited the cell viability, migration and invasion of MDA-MB-231 cells, as well as chemotactic migration and heterogeneous adhesion of MDA-MB-231 cells to MG-63 cells, showing a synergetic effect. After gene silencing on TGF-β1 and FOXM1, the epithelial to mesenchymal transition (EMT) related molecules vimentin mRNA expression decreased while E-cadherin increased.Conclusion PDMAEMA-SS-PEG-SS-PDMAEMA was suitable for TGF-β1 siRNA and FOXM1 siRNA delivery, exhibiting synergetic inhibition effect on TNBC and its bone metastases, which might be related to its synergetic inhibition on EMT.
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DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2024
·
IF 6.1
Dengyue Chen,Jieli Yin,Chen Liu,Jiaqi Guo,Mao Li,Baoyin Chen,Xuewen Zhang,Bing Wang,Xuan Zhu
Both chemodynamic therapy and photodynamic therapy, based on the production of reactive oxygen (ROS), have excellent potential in cancer therapy. However, the abnormal redox homeostasis in tumor cells, especially the overexpressed glutathione (GSH) could scavenge ROS and reduce the anti-tumor efficiency. Therefore, it is essential to develop a simple and effective tumor-specific drug delivery system for modulating the tumor microenvironment (TME) and achieving synergistic therapy at the tumor site. In this study, self-assembled nanoparticles (named CDZP NPs) were developed by copper ion (Cu2+), Doxorubicin (Dox), Zinc phthalocyanine (ZnPc) and a trace amount of poly (2-(di-methylamino) ethylmethacrylate)-poly [(R)-3-hydroxybutyrate]-poly (2-(dimethylamino) ethylmethacrylate (PDMAEMA-PHB-PDMAEMA) through chelation, π-π stacking and hydrophobic interaction. This triple factors-responsive (pH, laser and GSH) nanoparticles demonstrated unique advantages through the synergistic effect. Highly controllable drug release ensured its effectiveness at the tumor site, Dox-induced chemotherapy and ZnPc-mediated fluorescence (FL) imaging exhibited the distribution of nanoparticles. Meanwhile, Cu2+-mediated GSH-consumption not only reduced the intracellular ROS elimination but also produced Cu+ to catalyze hydrogen peroxide (H2O2) and generated hydroxyl radical (∙OH), thereby enhancing chemodynamic and photodynamic therapy. Herein, this study provided a green and relatively simple method for preparing multifunctional nanoparticles that can effectively modulate the TME and improve synergetic cancer therapy.
研究领域:
纳米技术
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癌症治疗
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药物递送系统
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肿瘤微环境
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活性氧
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协同治疗
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Inorganic and Organometallic Polymers and Materials
·
2024
·
IF 3.9
Liu Xuemei,Wang Peng,Wan Mengting,Li Songjun,Zhang Yuan
In this study, the photoresponsive monomer of 2-nitrobenzyl methacrylate (NBMA) was introduced into the dispersion polymerization of benzyl methacrylate (BzMA) monomer mediated by poly[ N , N -(dimethylamino) ethyl methacrylate] (PDMAEMA) macro-RAFT agent, and the PDMAEMA- b -P(BzMA- co -NBMA) block copolymer nanoparticles were prepared. The effects of the introduction of NBMA on the polymerization kinetics and the morphology of formed copolymer nanoassemblies were studied in detail. Then, the light response of PDMAEMA- b -P(BzMA- co -NBMA) block copolymer nanoparticles was verified. Moreover, the copolymer nanoparticles after irradiation could be used as the templates for loading silver nanoparticles, and it is proved that the template has advantage of regulating the amount of loaded silver by changing the UV light irradiation time. Graphical Abstract
研究领域:
高分子科学
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材料科学
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纳米技术
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光化学
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产品关联
DOI
高影响因子
Journal of Materials Chemistry B
·
2018
·Vol. 6
·期 48
·8170-8179
·
IF 4.776
Yu Cheng,Chao Huang,Dian Yang,Kai Ren,Jie Wei
Bilayer-type hydrogel composites that respond to multiple stimuli provide a useful approach for manufacturing soft actuators and intelligent sensors. In this study, we propose two types of bilayer hydrogel composites (referred to as “N” type and “D” type). The “N” type and the “D” type separately consist of poly(N-isopropylacrylamide) (PNIPAM) and poly(2-(dimethylamino)ethyl methacrylate) (PDMAEMA) bilayer hydrogels, with acrylamide (AM) as the co-monomer. The “N” type hydrogel composite exhibits oriented bending as temperature increases or as environmental salt concentration increases. The “D” type also presents oriented bending as temperature increases. Additionally, it demonstrates bending and changes color based on the pH of the surrounding solution, since it is doped with an acid-based pH-indicator. In a demonstration, we present several straight rods composed of a mixture of “N” and “D” type hydrogel composites that are able to morph into the letters “SOS” under environmental changes (temperature or pH). This can be used as a visual environmental indicator, or as an alarm. Furthermore, we show structures composed of a mixture of “N” and “D” composites imitating the flexible motions of a ballet dancer or a Tai Chi master. These mixed bilayer polymer composites may be used for applications in soft robotics, biomimetic devices, and environmental sensors.
研究领域:
材料科学
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软体机器人
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环境传感
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF MATERIALS SCIENCE & TECHNOLOGY
·
2022
·
IF 10.319
Liwei Sun,Lingjie Song,Xu Zhang,Shuaishuai Yuan,Shifang Luan
Hierarchical polymer brushes have been considered as an effective and promising method for preventing implant-associated infections via multiple antibacterial mechanisms. Herein, a bacterial phosphatase responsive surface with hierarchical zwitterionic structures was developed for timely dealing with the polymeric implant-associated bacterial infection. The hierarchical polymeric architecture was subtly realized on model polypropylene (PP) substrate by sequential photo living grafting of poly (2-(dimethylamino) ethyl methacrylate (PDMAEMA) bottom layer and zwitterionic poly (sulfobetaine methacrylate) (PSBMA) upper layer, followed by the conversion of the PDMAEMA into the zwitterionic structure via successive quaternization and phosphorylation reactions. Owing to shielding the bottom polycations, the hierarchical zwitterionic polymer brushes guaranteed the surface with the optimal biocompatibility under the normal physiological environment. Once bacteria are invaded, the surface bactericidal activity of the bottom layer can be rapidly and automatically activated owing to the transition triggered by bacterial phosphatase from zwitterion to polycation. Additionally, ameliorated by the upper layer, the hierarchical surface showed obvious adhesion resistance to dead bacterial cells and notably migrated the cytotoxicity of exposed polycation after completion of the bactericidal task. As a proof-of-principle demonstration, this self-adaptive hierarchical surface with sensitive bacterial responsiveness and biocompatibility showed great potential in combating hernia mesh-related infection. This work provides a promising and universal strategy for the on-demand prevention of medical device-associated infections.
研究领域:
材料科学
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生物材料
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聚合物化学
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生物医学工程
·
感染控制
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）