SCIENCE OF THE TOTAL ENVIRONMENT
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2023
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IF 9.8
Wangjing Zhai,Wenqi Jiang,Qiqi Guo,Zhixuan Wang,Donghui Liu,Zhiqiang Zhou,Peng Wang
Due to sewage irrigation, manure fertilizer application or other agricultural activities, antibiotics have been introduced into farmland as an emerging contaminant , existing with other agrochemicals . However, the potential influences of antibiotics on the efficiency of agrochemicals and crops health are still unclear. In this work, the effect of antibiotics on fertilization efficiency and pea yield was evaluated, and the mechanism was explored in view of soil microbiome. Nitrogen utilization and pea yield were decreased by antibiotics. In specific, the weight of seeds decreased 9.5 % by 5 mg/kg antibiotics and decreased 25.1 % by 50 mg/kg antibiotics. For N nutrient in pea, antibiotics resulted in 62.5 %–63.7 % decrease in amino acid content and 8.3 %–35.3 % decrease in inorganic-N content. Further research showed that antibiotics interfered with N cycle in soil, inhibiting urea decomposition and denitrification process by reducing function genes ure C, nir K and nor B in soil, thus decreasing N availability. Meanwhile, antibiotics destroyed the enzyme function in N assimilation. This work evaluated the environmental risk of antibiotics from fertilization efficiency and N utilization in crop. Antibiotics could not only affect N cycle, limiting the decomposition of N fertilizer, but also affect N utilization in plants, thus affecting the yield and even the quality of leguminous crops.
研究领域:
农业
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环境科学
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微生物学
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植物科学
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产品关联
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
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2022
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IF 10.753
Xuqian Zhang,Ying He,Zitong Yuan,Guangmao Shen,Zan Zhang,Jinzhi Niu,Lin He,Jinjun Wang,Kun Qian
Thiacloprid is a new chlorinated nicotinoid insecticide against stinging-oral pests, such as aphids. It is less toxic to bees but more toxic to earthworms. In this study, a pH- and amylase-responsive MOF (ZIF-8) was constructed for site-specific delivery of thiacloprid to control pea aphids and more safety for earthworms. Thiacloprid from α-cyclodextrin@Thiacloprid@ZIF-8 (α-CD@T@ZIF-8) could be released quickly in pea aphids, which was ascribed to disintegration of ZIF-8 at low pH values in pea aphid intestines and degradation of α-CD under the action of α-amylase. The release results showed a significant pH dependence of α-CD@T@ZIF-8, with an approximately 65 % release amount at pH = 7 and a 95 % release amount at pH = 5 for 7 d. The results of the pot experiment and biosafety showed that for α-CD@T@ZIF-8, 88 % pea aphids could be killed compared with 32 % aphids for commercially available formulation on the 7th day after application. Meanwhile the LC 50 of thiacloprid OD was 0.034 μg/cm 2 and the LC 50 of α-CD@T@ZIF-8 was 0.564 μg/cm 2 on earthworms, and it was more safety for pea and lower acute toxicity and enrichment for the earthworms. α-CD@T@ZIF-8 could be used for intelligently controlled release of other insecticides against aphids.
研究领域:
农业科学
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环境科学
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昆虫学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
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2022
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IF 9.231
Lijuan Luo,Zhiming Wang,Yuanyuan Deng,Zhencheng Wei,Yan Zhang,Xiaojun Tang,Guang Liu,Pengfei Zhou,Zhihao Zhao,Mingwei Zhang,Ping Li
High-pressure homogenization (HPH) is a technique that impacts the aggregation of globular proteins. In this study, the effect of HPH (at a pressure of 30/50 MPa for three cycles) was investigated on the aggregation states and functional properties of insoluble commercial pea protein isolates (CPPI). Results showed that HPH significantly improved the solubility, foaming and emulsifying capacity of CPPI. Samples treated at 50 MPa demonstrated better foaming and emulsifying capacity than that at 30 MPa. Surface hydrophobicity, intrinsic fluorescence, SDS-PAGE and FTIR analysis revealed that insoluble precipitates/aggregates (most legumins included) of CPPI were broken down and converted into soluble aggregates. Low-pressure HPH (30 MPa) can break non-covalent bonds (hydrophobic interactions), whereas higher pressure (50 MPa) can further break covalent bonds (S S). The study sheds light on the mechanism of disruption of insoluble CPPI under HPH and proposes a method to enhance their techno-functional properties for application in food formulations.
研究领域:
食品科学
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蛋白质化学
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DOI
PubMed
高影响因子
Frontiers in Nutrition
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2022
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IF 6.59
Hou Wenbo,Long Jie,Hua Yufei,Chen Yeming,Kong Xiangzhen,Zhang Caimeng,Li Xingfei
The emulsion gels that can be used as solid fat replacers were produced with different polysaccharides (κ-carrageenan, κC; high-acyl gellan, HA; konjac glucomannanon, and KGM), pea protein isolate (PPI) and sunflower seed oil. The effect of polysaccharide concentration on the texture, rheological property, microstructure, and water holding capacity of the mixed emulsion gels were investigated. Rheological results showed that the presence of polysaccharides enhanced the hardness, storage modulus and resistance against deformation of emulsion gel, where PPI/κC system exhibited superior hardness with a similar level of pig back fat, due to the self-gelation behavior of κC. CLSM and SEM results showed that the presence of κC, HA, and KGM broke the uniform structure of gel network and formed irregular, threadlike, and oval shaped inclusions respectively, resulting in the broken and coalescence of oil droplets. The α-helix content of emulsion gels decreased, while β-sheet, β-turn and random coils slightly increased due to the unfolding of protein during gel formation. This study may offer a valuable strategy for the development of solid fat mimetic with the characteristics closing to the pig back fat.
研究领域:
食品科学
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材料科学
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化学工程
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产品关联
DOI
PubMed
高影响因子
NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL
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2023
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IF 2.9
Nina Qin,Yan Meng,Zhihua Ma,Zhaoping Li,Zhenzhen Hu,Chenyi Zhang,Liyong Chen
The previous documentation has shown the role of resistant starch in promoting intestinal health, while the effect of starch-lipid complex (RS5) on colitis remains unclear. This study aimed to investigate the effect and potential mechanism of RS5 in colitis. We prepared RS5 complexes by combining pea starch with lauric acid. Mice with dextran sulfate sodium-induced colitis were treated with either RS5 (3.25 g/kg) or normal saline (10 mL/kg) for seven days, and the effects of pea starch-lauric acid complex on mice were observed. The RS5 treatment significantly attenuated weight loss, splenomegaly, colon shortening, and pathological damage in mice with colitis. Compare with the DSS group, cytokines levels, such as tumor necrosis factor-α and interleukin-6 in both serum and colon tissue was significantly decreased in RS5 treatment group, while the gene expression of interleukin-10 and the expression of mucin 2, zonula occludens-1, Occludin, and claudin-1 in the colon was significantly upregulated in RS5 treatment group. In addition, RS5 treatment altered the gut microbiota structure of colitis mice by increasing the abundance of Bacteroides and decreasing Turicibacter, Oscillospira, Odoribacter, and Akkermansia. The dietary composition could be exploited to manage colitis by attenuating inflammation, restoring the intestinal barrier, and regulating gut microbiota.
研究领域:
胃肠病学
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免疫学
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营养学
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微生物学
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产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
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2023
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IF 11.2
Fuyue Dong,Wei Gao,Pengfei Liu,Xuemin Kang,Bin Yu,Bo Cui
In this study, microcrystalline butyrylated pea starch (MBPS) with higher contents of resistant starch (RS) was synthesized via esterification with butyric anhydride (BA) using microcrystalline pea starch (MPS) as the raw material. With the addition of BA, the new characteristic peaks appeared at 1739 cm −1 and 0.85 ppm obtained from FTIR and 1 H NMR, respectively, and increased with the higher degree of BA substitution. Moreover, an irregular shape of MBPS, such as condensed particles and more cracks or fragments, had been observed by SEM. Further, the relative crystallinity of MPS increased then native pea starch and decreased with the reaction of esterification. MBPS had higher decomposition onset temperature (T o ) and temperature of maximum decomposition (T max ) with increasing DS values. Simultaneously, an increasing trend RS content from 63.04 % to 94.11 % and a decreasing trends in rapidly digestible starch (RDS) and slowly digestible starch (SDS) contents of MBPS were recorded with increasing DS values. MBPS samples showed higher production capacity of butyric acid ranging from 553.82 μmol/L to 892.64 μmol/L during the fermentation process . Compared with MPS, the functional properties of MBPS were significantly improved.
研究领域:
食品科学
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化学
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营养学
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产品关联
DOI
PubMed
高影响因子
SOUTH AFRICAN JOURNAL OF BOTANY
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2026
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IF 3.1
Farwa Basit,Saghir Abbas,Javaid Akhter Bhat,Pongsakorn Nitmee,Parvaiz Ahmad,Abdulaziz Abdullah Alsahli
Vanadium (V) toxicity impairs pea plants' secondary metabolites, nutrient acquisition, and photosynthesis. • V toxicity increases oxidative stress biomarkers in rice plants. • Melatonin (MT) mitigates V toxicity and promotes growth. • MT improves nutrient acquisition and antioxidant activity in rice leaves under V stress. • MT regulates phytohormone-related genes, enhancing rice biomass and growth. Vanadium (V) stress is a potential challenge for sustainable crop production and healthy plant growth. The potential of melatonin (MT) in V stress mitigation has remained unexplored in the pea plant. In the present study, we investigated the role of exogenous melatonin (MT1; 45 µM, MT2; 75 µM) application in diminishing the adverse effect of V exposure (100 µM) in pea seedlings. Our results showed that V stress negatively affected the germination, biomass, photosynthetic attributes, secondary metabolites, and nutrient acquisition, as well as increased oxidative stress. Moreover, V stress has altered the expression of phytohormone-related genes via enhanced uptake of V. Conversely, the supplementation of MT significantly reversed the negative effect of V stress. Furthermore, MT2+V treatment decreased the malondialdehyde (MDA) and hydrogen peroxide (H 2 O 2 ) content and improved the photosynthetic attributes, rate of respiration, total phenol, and flavonoid content. Besides, MT2+V enhanced the antioxidant defense system activities, as well as the nutrient content (K, Mg, and Zn) while decreasing the V uptake. The MT2+V treatment also modulated the expression levels of phytohormone-related genes in pea seedlings, with LOX1, LOX2, ACS3 , and JAR1 being upregulated, while OPR1 and NCED 2 were downregulated, which might enhance the plant defense against overproduction of reactive oxygen species (ROS). In conclusion, the present study provided the key insights about the possible role of the MT in the modulation of V stress in pea plants, as well as its use as a priming agent in the alleviation of heavy metal stresses in crop plants.
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2026
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IF 10.4
Yan Yin,Siqi Wang,Jiangtao Yu,Xiaohui Ma,David Julian McClements,Long Jin,Cuicui Ma,Xuebo Liu,Fuguo Liu
Pea protein isolate (PPI) has limited use in the food industry due to its poor solubility and gelation. This study used electron beam irradiation (EBI, 5–30 kGy) to treat PPI, followed by dual crosslinking with transglutaminase (TG) and/or CaCl 2 to prepare oil-in-water emulsion gels, aiming to optimize processing conditions for improved gelation properties. Moderate EBI (10–15 kGy, with 15 kGy showing the greatest overall improvement) promoted protein unfolding and increased surface hydrophobicity, water solubility (10.6% → 11.2%), and emulsifying activity index (15.9 → 25.6 m 2 /g). Dual crosslinking (TG + 25 mM Ca 2+ ) generated stronger, more elastic networks than single crosslinking, with higher water-holding capacity (80.7%), superior freeze-thaw stability, and higher viscoelasticity. EBI-treated PPI produced softer yet more hydrated gels. Excessive Ca 2+ (≥50 mM) or irradiation (20–30 kGy) increased protein aggregation and weakened gel strength. This combined physical-enzymatic crosslinking strategy allows tailoring of plant-based emulsion gels for specific food applications. Download: Download high-res image (320KB) Download: Download full-size image
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DOI
PubMed
高影响因子
Innovative Food Science & Emerging Technologies
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2025
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IF 6.8
Xiaoyuan Wang,Yue Zhao,Megan Povey,Xiaopeng Wei,Lihua Zhang,Wei Zong
Ultrasonic pre-treatment can significantly accelerate the reaction rate of glycosylation, improve the efficiency of protein modification, and avoid the disadvantage of long-time required by traditional glycosylation. Moreover, this method has mild reaction conditions, which can reduce the destruction of essential amino acids. Therefore, this study aimed to modify pea protein isolate (PPI) with ultrasound-assisted glycosylation by xylose. The effects of protein-to-xylose ratios on the structural and functional properties of PPI were systematically examined. The results demonstrated that ultrasonic pretreatment could improve the glycosylation grafting efficiency, and the structure and properties of modified PPI (UPPIXs) underwent significant changes. Modified PPI with protein-to-xylose ratio of 2:1 (UPPIX-2:1) exhibited superior solubility, emulsifying and foaming characteristics. Subsequently, UPPIXs were used to fabricate lutein-loaded nanoparticles (UPPIXs-LUT), and their characteristics were analyzed. The results indicated that nanoparticle prepared with UPPIX-2:1 displayed the highest encapsulation efficiency (89.46 %). Spectral analyses, fluorescence quenching, and thermodynamic studies confirmed that hydrophobic interactions dominated nanoparticle formation. Antioxidant activity and stability assessments showed that UPPIXs-LUT possessed excellent antioxidant properties, and improved stability under light, heat, storage, and pH. This study provides a theoretical foundation to use self-assembled glycosylated protein nanoparticles as an efficient delivery system for hydrophobic nutrients.
研究领域:
食品科学
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纳米技术
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蛋白质工程
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超声处理
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营养递送系统
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
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IF 7.7
Qian-Da Xu,Zhi-Long Yu,Qiang He,Wei-Cai Zeng
Pea protein isolate (PPI) was used as a carrier matrix to load tannic acid (TA) due to its multiple cavity structures and reaction sites, after that, magnesium ion (M) was further added to form more stable carrier structures. PPI was covalently bound with TA to form TA-PPI complexes in alkaline conditions, then M induced the aggregation of TA-PPI to produce M-TA-PPI complexes. TA mainly interacted with free amino groups and sulfhydryl groups of PPI, thereby decreasing their content in complexes. TA further decreased the α-helix content and increased the β-sheet and β-turn content in TA-PPI complexes correspondingly, nevertheless the M would decline these changes in M-TA-PPI complexes. As a result of binding, TA and M jointly increased the average molecular size of complexes. The higher TA addition amount (10–20 mg/g PPI) was conducive to the stronger intramolecular interactions (more hydrophobic interactions and disulfide bonds), gel structure (higher hardness value) and storage modulus in M-TA-PPI gels. Compared with TA-PPI complexes, M-TA-PPI complexes showed higher stability in gastric digestion and higher TA releasement and antioxidant capacity of its digesta in intestinal digestion. This kind of metal-phenolics-protein complexes may have potentials to be a stable and efficient carrier for loading gastric sensitive polyphenols.
研究领域:
食品科学
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生物技术
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营养学
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产品关联
DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
·
2024
·
IF 11
Wenhan Li,Shuang Han,Hechun Huang,David Julian McClements,Shuai Chen,Cuicui Ma,Xuebo Liu,Fuguo Liu
Plant proteins are becoming increasingly popular as functional ingredients in foods because of their health, sustainability, and environmental benefits. The functional performance of plant proteins can often be improved by combining them with other food ingredients. For instance, they can be combined with polyphenols and metal ions to form surface active protein-polyphenol-iron complexes with biological activity. In this study, ternary complexes of pea protein isolate (PPI), epigallocatechin-3-gallate (EGCG) and iron (Fe 3+ ) was prepared using a simple molecular self-assembly method. The formation mechanism, characteristics, and functional properties of these complexes were then characterized. Our results showed that PPI, EGCG, and Fe 3+ could spontaneously combine to form nano-sheet networks held together by hydrogen bonds, phenolic hydroxyl coordination bonds, and electrostatic interactions. The addition of Fe 3+ increased the fraction of disordered secondary structures, reduced the thermal denaturation temperature, reduced the surface hydrophobicity, increased the particle size, and altered the surface potential of the proteins in a dose dependent manner. Optimizing the EGCG-to-Fe 3+ ratio in the complexes (1:2 w/w) led to the formation of protein composites with improved surface, antioxidant, and antibacterial activities. This study provided valuable insights into the interaction mechanisms of proteins, polyphenols, and metal ions in ternary complexes. These complexes may be used as multifunctional ingredients in plant-based foods because of their beneficial antioxidant, antimicrobial, nutritional, and emulsification properties.
研究领域:
食品科学
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生物化学
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DOI
高影响因子
FOOD HYDROCOLLOIDS
·
2024
·
IF 11
Zhong Han,Ze-Ling Chen,Xin-Dong Xu,Songyi Lin,Yan Wang,Lang-Hong Wang,De-Bao Niu,Xin-An Zeng,Meng-Wai Woo,Ruo-Yong Wang
The development of natural polyphenol-protein complexes for preserving surimi holds great promise. The effects of pea protein isolate (PPI) - (−)-Epigallocatechin-gallate (EGCG) non-covalent and covalent complexes modified by pulsed electric field (PEF) on the preservation of tilapia surimi were evaluated. Results showed that the PEF-treated PPI-EGCG non-covalent complex extended the shelf life of surimi by more than 6 days. This complex reduced the microbial activity and delayed the lipid and protein oxidation of surimi during chilled storage. Total viable count (TVC), thiobarbituric acid reactive substances (TBARS), and total volatile base nitrogen (TVB-N) of surimi with PEF-treated PPI-EGCG non-covalent complex on day 15 were 31.65 %, 68.67 %, and 54.46 % lower than untreated surimi, respectively. Moreover, the rate of colour deterioration and off-odour development were also delayed. The major products of lipid oxidation in surimi were reduced by 37.91 % for hexanal , 43.14 % for pentanal, 73.24 % for 1-octen-3-ol, and 39.32 % for 1-penten-3-ol on day 15. Structural analyses and computer simulations revealed that PEF was able to unfold the structure of PPI, thereby exposing more binding sites for EGCG. PEF-treated PPI-EGCG non-covalent complex allowed the slow dissociation of EGCG from PPI and maintained the antimicrobial and antioxidant activities of EGCG in a long storage time. Therefore, PEF-treated PPI-EGCG non-covalent complex was considered as a potential preservative in fish and fish products.
研究领域:
食品科学
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生物技术
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保鲜技术
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）