EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
·
2020
·
IF 3.616
Jiahui Sun,Jing Li,Qi Liu,Min Jiang,Mengjia Yang,Siwen Zhan,Tong Qiu,Kaiyong He,Xueqiong Zhang
This study aimed to develop, evaluate, and optimize the mPEG-PLA/vitamin E-TPGS mixed micelle drug delivery system to encapsulate celecoxib (CXB) and honokiol (HNK) for intravenous treatment of breast cancer. To this end, we formulated CXB-loaded mPEG-PLA/vitamin E-TPGS (PV-CXB) and HNK-loaded mPEG-PLA/vitamin E-TPGS (PV-HNK) mixed micelles and analyzed their characteristics. The 4T1 cell line was used for cytotoxicity determination and cellular uptake experiments, and for establishing a 4T1-bearing mouse model for histopathology, immunofluorescence, terminal deoxynucleotidyl transferase-mediated nick end labeling, and Western blot analysis . The synergistic effects of PV-CXB and PV-HNK combination therapy were investigated in vitro and in vivo using the coefficient of drug interaction values. The mean size of PV-CXB and PV-HNK prepared with optimal formulation was approximately 50 nm , with a spherical shape. PV-CXB and PV-HNK combination therapy exhibited cytotoxicity in 4T1 cells in vitro. However, the toxicity of PV-CXB and PV-HNK combination therapy was not apparent in normal tissues (heart, liver, spleen, lung, and kidney) in vivo and reduced the expression of collagen fibers in tumor tissues. Moreover, the combination therapy reduced the expression of tumor growth biomarkers (Foxp3, CD4, Gr-1, CD11b, CD31, Ki67, FoxM1, and VEGF). In addition, the tumor cell apoptosis rate reached 45.71 ± 0.62%. The combined treatment with PV-CXB and PV-HNK showed synergistic effect both in vitro and in vivo. Thus, the PV-CXB and PV-HNK drug delivery system could be used as a potential combination therapy for breast cancer .
研究领域:
药剂学
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肿瘤学
·
生物医学工程
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产品关联
DOI
PubMed
高影响因子
MACROMOLECULAR RESEARCH
·
2011
·Vol. 19
·期 12
·1287-1295
·
IF 1.639
Xu Feng,Yan Ting-Ting,Luo Yan-Ling
The thermo-sensitive amphiphilic block copolymer poly( N -isopropylacrylamide)- block -poly( D,L -lactide) (PNIPAAm- b -PLA) was synthesized using a simple free radical copolymerization route based on a bifunctional initiator, 2,2-azobis(2-methylpropion amidine) dihydrochloride followed by the ring-opening polymerization of D,L -actide in the presence of a Sn(Oct) 2 catalyst. The chemical structure of the PNIPAAm- b -PLA copolymers was verified using Fourier transform-infrared spectrophotometry and nuclear magnetic resonance, and the molecular weight and polydispersity index were examined using gel permeation chromatography. The amphiphilic PNIPAAm- b -PLA block copolymers could self-assemble into spherically shaped micelles in an aqueous solution with a transmission electron microscopy diameter range of 40–56 nm and a dynamic laser scattering hydrodynamic diameter of 90–200 nm. This behavior depends on the environmental temperature, the hydrophobic interactions among PNIPAAm molecular chains, the intermolecular hydrogen bonding between the PNIPAAm chains and water molecules, and the intramolecular hydrogen bonding between the -CONH 2 groups. The copolymers held a critical micellization concentration of 4.93–7.21 mg·L −1 and a low critical solution temperature of 31.15–32.62 °C being more or less affected by their compositions, PLA or PNIPAAm block length, and polymerization temperature. The as-prepared PNIPAAm- b -PLA block polymers are anticipated to be applied as candidate drug release carriers.
研究领域:
高分子化学
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药物递送系统
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材料科学
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产品关联
DOI
高影响因子
POLYMER ENGINEERING AND SCIENCE
·
2024
·Vol. 64
·期 11
·5737-5749
·
IF 3.2
Yuhang Wang,Hongyan Fu,Jing Tan,Mahmoud M. Bubakir,Xiaodong Xu,Haoyi Li,Weimin Yang
The electrospun yarn for sutures has gained worldwide attention due to its fine fibers that resemble the extracellular matrix and its abundant functional sites. However, the use of a large number of toxic solvents poses safety risks and environmental pollution during production, making it challenging to directly apply electrospun yarn in the biomedical field. In this study, an environmentally friendly and safe method without toxic solvents was proposed for preparing antibacterial PLA ultrafine fiber sutures. This method involves melt electrospinning, hot-stretching, low-temperature plasma treatment, and chitosan grafting. The PLA ultrafine fiber sutures exhibit a high tensile strength which is 2.04 N before knotting and 1.57 N after knotting. The suture diameter is 118.7 μm and average fiber diameter is 1.72 μm. Chitosan grafted on the fiber surface provides excellent antibacterial properties for the sutures, with antibacterial rates against Escherichia coli and Staphylococcus aureus reaching 99.89% and 99.11%, respectively. The cell survival rate is 97.33%, while hemolysis rate is only 2.35%, demonstrating excellent biocompatibility of PLA ultrafine fiber sutures. This study presents a green and safe method for preparing clinically applicable absorbable antibacterial surgical sutures with high mechanical properties. Highlights A green and safe method for preparing PLA-CS ultrafine fiber sutures. PLA-CS suture with a tensile strength of 2.04 N and a fiber diameter of 1.72 μm. PLA-CS suture with good antibacterial property and biocompatibility.
研究领域:
生物医学工程
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材料科学
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医学
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产品关联
DOI
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
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2018
·
IF 3.997
Bowen Yu,Lu Meng,Sirui Fu,Zhiyu Zhao,Yuhang Liu,Ke Wang,Qiang Fu
The applications of Polylactide (PLA) microspheres in biomedical areas are greatly determined by the size, morphology and internal structure. Taking advantage of the formation of stereocomplex (SC) crystallites between poly(L-lactide) (PLLA) and poly(D-lactide) (PDLA), we propose a facile strategy to prepare PLA microspheres with tunable morphology and crystalline structure by compounding PLLA and PDLA. With increasing PDLA content, the crystallinity of SC-PLA rose gradually until the ratio of PLLA and PDLA reached 1:1 and then fell. Correspondingly, the morphology of the microspheres were varied (smooth, porous, golf-ball like, guava like) and higher crystallinity of SC-PLA would lead to a more coarse and porous structure. Finally, three typical kinds of Rifampicin-loaded microspheres with different ratio of PLLA and PDLA (7:3, 3:7, 10:0, sorted by porosity from high to low) were prepared and the release behavior was compared. At 30 h, the cumulative release of 7:3, 3:7 and 10:0 microspheres were 32.6%, 17.8% and 6.0% respectively, indicating that the release profiles were generally determined by the porosity of the microspheres. Our findings not only provide a new strategy to prepare PLA microspheres with controllable morphology but offer additional possibilities for the applications of SC-PLA products in biomedical area.
研究领域:
生物医学工程
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药学
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材料科学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF MATERIALS SCIENCE
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2014
·Vol. 49
·期 22
·7730-7741
·
IF 2.305
Luo Yan-Ling,Huang Rui-Juan,Xu Feng,Chen Ya-Shao
pH-Sensitive biodegradable polymethacrylic acid- block -polylactic acid- block -polymethacrylic acid (PMAA 2 - b -PLA- b -PMAA 2 ) H-type multiblock copolymers were synthesized by atom transfer radical polymerization. The copolymer structure and molecular weight were characterized by FT-IR, 1 H NMR, and gel permeation chromatography. The physicochemical characterization revealed that the copolymers could spontaneously form spherical core–shell micelles in aqueous solution, with critical aggregation concentration of about 19.7–32.5 mg L −1 and the hydrodynamic diameters below 200 nm. Z eta potentials measurements disclosed that the copolymer micelles were negatively charged due to ionized carboxyl groups in various PBS solutions. The H-type block copolymer micelles exhibited pH- sensitivity, as expected; and the hydrophobic anticancer drugs, 10-hydroxycamptothecin, and paclitaxel, had faster release rate in PBS solution of pH 5.6–7.4 than in PBS of pH 1.4, which was important for applications in the therapy of small intestine cancers. The copolymer micelle aggregates were proved to be biodegradable, and the degradation rates changed with copolymer compositions and environmental media. The micelle drug formulation indicated pH-dependent cytotoxicity and was thus capable of effectively killing the intestinal cells while avoiding doing harm to stomach. The biodegradable pH-sensitive PMAA 2 - b -PLA- b -PMAA 2 H-type copolymer micelles can be used as water-insoluble drug targeting release carriers for targeted treatment of intestine cancers.
研究领域:
生物材料学
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药剂学
·
高分子化学
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产品关联
DOI
高影响因子
JOURNAL OF APPLIED POLYMER SCIENCE
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2025
·
IF 2.8
Shuai Li,Mo Niu,Haoshui Yu,Xueying Song
Bio-degradable plastics have gained significant attention due to their biodegradability and environmental friendliness. However, how to improve the degradation rate of PBAT/PLA composites in aqueous environments is still a challenge. In this study, a green processing technology was proposed to achieve the rapid degradation of PBAT/PLA. By using lignin as an intermediate medium and adopting a continuous batch feeding strategy, the compatibility and biodegradability of PBAT/PLA composite materials are simultaneously improved in the absence of compatibilizers. The resulting PBAT/PLA/lignin films demonstrate excellent mechanical, water barrier, and degradation properties. The elastic modulus ( E ), tensile strength ( σ b ) and elongation at break ( ε b ) of PBAT/PLA/2%lignin are 124.2 MPa, 45.5 MPa, and 737%, respectively. The weight loss rate of the PBAT/PLA/10%lignin film after hydrolysis for 60 h was 30.0 wt%, which was 316% higher than that of the PBAT/PLA film. This sustainable and green processing strategy promotes the application of lignin in the field of composite materials, while solving the problems of poor mechanical properties and slow hydrolysis rate of PBAT/PLA composite materials.
研究领域:
材料科学
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高分子科学
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环境科学
·
化学工程
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产品关联
DOI
高影响因子
COLLOID AND POLYMER SCIENCE
·
2016
·Vol. 295
·期 1
·259-266
·
IF 1.89
Shi Yanan,Zhu Haibo,Ren Yan,Li Keke,Tian Baocheng,Han Jingtian,Feng Dongxiao
Poly(ethylene glycol)-poly(D,L-lactide) (PEG-PLA), a type of copolymer widely used for drug delivery, was synthesized in the present work based on previous studies, which is used for bovine serum albumin (BSA)-loaded micelle formulation. The micelles exhibited less than 100 nm in size with narrow polydispersity indices (PDI) of smaller than 0.3, and showed an encapsulation efficiency of 69%. BSA was encapsulated in the PEG-PLA micelle successfully exhibiting intact structure indicated by native polyacrylamide gel electrophoresis (Native-PAGE), fluorescence spectroscopy and fourier transform infrared spectroscopy (FTIR) analysis methods. It investigated the effects of freeze-drying, varying amount of cryoprotectants (sucrose, mannitol and lactose), and ultrasonication treatment on the size, and size distribution. The micelles were agglomerated in the freeze-drying process demonstrated by transmission electron microscope (TEM) and dynamic light scattering (DLS) analysis. Five percent of mannitol and ultrasonication for 90 s were found producing a significant reduction in agglomeration as well as a sharp decrease in size. The results demonstrated suitable cryoprotectant, and ultrasonication are beneficial to control the size and distribution of protein-loaded PEG-PLA micelle.
研究领域:
药剂学
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药物递送
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聚合物科学
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蛋白质工程
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产品关联
DOI
高影响因子
POLYMER TESTING
·
2023
·
IF 5.1
Minzimo Song,Shenghua Li,Guiyou Zhu,Jianwei Guo
It is valuable research to improve the compatibility and toughness of PLA/PCL blends while endowing them with shape memory & 4D printing capability. In this work, lactic acid/caprolactone-modified chitosan (mCS) was used to connect the amorphous region of PLA and PCL to achieve compatibilisation, toughening and 4D printing shape memory ability. The effects of different mCS additions on the morphology, crystalline behaviour, and thermal properties of PLA/PCL blends were observed to study the mechanism of compatibilisation and toughening. Adding 0.5 g mCS into 10 g PLA/PCL blends had the best compatibilisation and toughening effect. At the same time, the blends with this addition ratio had the best shape memory performance after 4D printing. The shape fixity and shape memory ratios of the 4D printing model were as high as 100% and 94.5%, respectively. This work provides a new way to fabricate biomedical devices and bionic components with 4D printing.
研究领域:
材料科学
·
生物医学工程
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Wen Zhang,Hongming Liu,Li Yan,Xi Mei,Zikang Hou
Developing of the multifunctional polymeric carrier for controlled drug release is still one of most challenging task. In this work, a pH-responsive dual drug delivery system was designed and prepared based on the zeolitic imidazolate framework-8 (ZIF-8). The poly(lactic acid)/chitosan (PLA/CS) core-shell nanofiber membranes by emulsion electrospinning, which the hydrophilic drug (Astragalus Polysacharin, APS) was encapsulated in the CS core and the hydrophobic drug (Camptothecin, CPT) was loaded into the PLA shell, respectively. Subsequently, ZIF-8 nanoparticles served as the protective layer were immobilized on the surface of PLA/CS to form multi-structural PLA/CS@ZIF-8 nanofiber membranes. In vitro drug release of nanofiber membranes were studied in the acidic and neutral medium, respectively. The results were that the hydrophilicity and surface roughness of nanofiber membranes rose with increasing of 2-MIM concentrations. The nanofiber membranes also had excellent pH-responsive and controlled release property. Furthermore, the drug release of PLA/CS@ZIF-8 for either APS or CPT were all carried out in a coexisting manner of diffusion and skeleton corrosion. In addition, in vitro cytotoxicity assay indicated nanofiber membranes with good cytocompatibility. Therefore, the multi-structured PLA/CS@ZIF-8 nanofiber membranes has been used as a potential pH-responsive dual drug release system.
研究领域:
材料科学
·
药物递送
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Xiaofang Liu,Yong Liu,Min Fan,Kunpeng Qian,Wenya Ma,Dan Li,Li Li
Starch is a natural polymer material that is widely sourced, biodegradable, and non-toxic. However, the application of starch is hindered by its hydrophilicity and lack of biological activity. In this study, ε-polylysine hydrochloride (ε-PLH) was grafted onto starch for improving antibacterial properties of starch and weakening its hydrophilicity. Subsequently, a new type of active packaging film was prepared by blending polylactic acid (PLA), polybutylene adipate terephthalate (PBAT) and grafted starch (ε-PLH-g-starch) using the melt extrusion method. ε-PLH-g-starch/PLA/PBAT film can effectively inhibit Escherichia coli and Staphylococcus aureus with inhibition rates of 48.62 ± 2.43 % and 57.31 ± 1.65 %, respectively. The addition of ε-PLH-g-starch also significantly improved the tensile strength, optical properties and barrier properties of the films. As a verification experiment, the application of ε-PLH-g-starch/PLA/PBAT film can effectively inhibit the protein hydrolysis, microbial growth and fat oxidation of beef, and extend the shelf life of beef at 4 °C from 7 to 14 days.
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产品关联
DOI
PubMed
高影响因子
APPLIED SURFACE SCIENCE
·
2018
·
IF 4.439
Zhenglong Yang,Kangyu Fu,Jing Yu,Xujing Shi,Peiting Zhou,Zhihao Cheng
Polylactide-b-fullerene-b-poly(3-hexylthiophene) (PLA- b -C 60 - b -P3HT) triblock copolymer via a combination of 1,3-dipolar cycloaddition reaction and controlled ring-opening polymerization technique was successfully synthesized. The structure, morphology and crystallinity of PLA- b -C 60 - b -P3HT copolymer were characterized by 1 H NMR, GPC, DSC, TGA, XRD, UV–vis, PL and AFM measurements. Nanoporous C 60 /P3HT films were easily prepared from PLA- b -C 60 - b -P3HT triblock copolymer by casting the copolymer followed by thermal annealing and chemical etching of the PLA chains. Fullerene molecules could self-assembly between P3HT and PLA to form a continuous film. An ordered nanoporous C 60 /P3HT film was formed in AFM by chemically etching the PLA microphase with NaOH solution. Photoluminescence analysis showed that the photoinduced electron transfer of the ordered nanoporous C 60 /P3HT film was significantly improved compared to the physically blending sample due to the increased interface between the P3HT and the C 60 .
研究领域:
材料科学
·
有机光电材料
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产品关联
DOI
高影响因子
Drug Delivery and Translational Research
·
2017
·Vol. 8
·期 1
·83-96
·
IF 3.094
Pan Linmei,Zhou Jing,Ju Feng,Zhu Huaxu
Alpha-asarone is a bioactive component of Acorus tatarincuii Schott with low bioavailability, which is often used for treatments of various brain diseases in clinical setting. This study was to formulate biodegradable methoxy polyethylene glycol-polylactic acid (mPEG-PLA) nanoparticles (NPs) surface-modified by lactoferrin (Lf), for delivering α-asarone into the brain following intranasal administration. Alpha-asarone NPs were prepared by premix membrane emulsification. The relative parameters were optimized by a Box-Behnken experimental design. The particle size, zeta potential, and dispersibility index of NPs and Lf-NPs were characterized. Their ex vivo permeation, pharmacokinetics, distribution in the brain and other tissue, brain targeting, and toxicity were investigated. Following intranasal administration, Lf-NPs had a better permeability and no significant poor bioavailability compared to NPs; the area under curve from 0 to 12 h of α-asarone in Lf-NPs of the olfactory bulb, hippocampus, olfactory bundles, and thalamus were 2.14-, 4.17-, 3.62-, and 1.96-fold of those in NP group, respectively. Lactoferrin could enhance the efficacy of brain targeting with NPs and reduce its liver accumulation. Toxicity of NPs on nasal mucosal cilia and epithelial cells was also decreased by Lf. To summarize, these results demonstrate that Lf-NPs of α-asarone have potential as a carrier for nose-to-brain delivery of α-asarone for brain diseases.
研究领域:
药剂学
·
神经科学
·
生物医学工程
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）