FOOD CONTROL
·
2024
·
IF 5.6
Lianjun Song,Meijun Lu,Puye Liang,Tiange Li,Xianqing Huang,Yan Ma,Libin Wan,Tianlin Wang
Herein, a ratiometric fluorescence aptasensor was established for zearalenone (ZEN) detection based on fluorescence resonance energy transfer (FRET) between aptamer-modified CdTe quantum dots (QDs) as donor and gold nanoparticles (AuNPs) as acceptor. The fluorescence of CdTe QDs quenched by AuNPs based on FRET, while Si QDs with blue fluorescence as reference. The aptamer-modified CdTe QDs were adsorbed to ZEN in the presence of ZEN, and the distance between the energy receptor and the donor increased, which prevented the FRET and led to the fluorescence recovering of CdTe QDs. The aptasensor exhibited a limit of detection of 3.5 pg/mL with a linear range of 0.01–100 ng/mL. In addition, a portable detection device was constructed by integrating with smartphone for point-of-care testing (POCT) of ZEN with satisfactory results, which could provide a promising application for visual POCT of ZEN in cereals.
研究领域:
分析化学
·
纳米技术
·
食品安全
·
即时检测
✓ 已验证
产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 4
·1-10
·
IF 5.3
Jin Birui,Ma Chuan,Zhang Chuyao,Yin Huiling,Zhao Guoxu,Hu Jie,Li Zedong
There is an urgent need for a point-of-care testing (POCT) method in developing and underserved regions to distinguish between two Monkeypox virus (MPXV) clades, given their varying transmissibility and clinical manifestations. In this paper, we target the specific complement protein gene fragment of two MPXV clades and construct a high-performance upconversion nanoparticles–based lateral flow assay (UCNPs-based LFA) with double T-lines and a shared C-line. This enables qualitative and quantitative dual-mode detection when combined with a smartphone and a benchtop fluorescence analyzer. The developed LFA exhibits stable performance, convenient operation, rapid readout (within 8 min), and a much lower limit of detection (LOD) (~ pM level) compared to existing POCT methods. The proposed detection platform demonstrates significant potential for pathogen diagnosis using a POCT approach. Graphical Abstract
研究领域:
病毒学
·
诊断方法
·
纳米技术
·
即时检测
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2023
·
IF 6.1
Birui Jin,Zhiguo Du,Jingcheng Ji,Yuemeng Bai,Deding Tang,Lihua Qiao,Jiatao Lou,Jie Hu,Zedong Li
Upconversion nanoparticles (UCNPs)-based fluorescence probes have shown great potential in point-of-care testing (POCT) applications, due to UCNPs’ features of high photostability and background-free fluorescence. Ceaseless improvements of UCNPs-probes have been carried out to increase detection sensitivity and to broaden detection range of UCNPs-based POCT. In this paper, we optimized UCNPs-probes by regulating probe density. The optimization was verified by a traditional lateral flow assay (LFA) platform for C-reactive protein (CRP) detection. Further, the optimized UCNPs-LFA integrating with a home-made benchtop fluorescence analyzer holds the capability to achieve high-performance POCT. Finally, nearly a 20 times sensitivity enhancement with a limit of detection of 0.046 ng/mL and a broad detection range of 0.2–300 ng/mL for CRP detection was obtained. Moreover, the optimized UCNPs-LFA was applied to detecting CRP in clinical serum samples and the detection results were consistent with the clinical test, validating its clinical practicability. The proposed optimization method is also expected to optimize other nanoparticles-based bio-probes for wider POCT application.
研究领域:
纳米技术
·
生物医学工程
·
分析化学
·
临床诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2022
·Vol. 414
·期 14
·4047-4057
·
IF 4.478
Jiang Shuai,Zhao Jie,Zhao Shifan,Deng Huizhen,Zhu Rui,Bai Yujian,Cui Guofeng
On-site screening of copper ions in body fluid plays a critical role in monitoring human health, especially in heavy pollution areas. In this study, we have developed a hybrid 3D printed integrated microdevice for the determination of copper ions in human body fluids. A fixed and low volume of sample was detected by using the integrated microdevice without any preprocessing. The hybrid channel enables sample uniform mixing and quantitative dilution with buffer solution by inducing the “horseshoe vortex” phenomenon. The electrolytic microcell based on the flow detection system shows a more effective copper ion reaction ratio and, as a result, a better sensitivity. The simulation of the finite element method (FEM) determined the relevant optimum parameters of the hybrid channel and the microcell. The design, fabrication, and detection procedure of the integrated microdevice are here illustrated. The microdevice presented superior detection properties towards copper ions. The calibration curves covered two linear ranges varying from 20 to 100 ppb and 100 to 400 ppb, respectively. The limit of detection was estimated to be 15 ppb ( S / N = 3). The relative standard deviation of the peak current measurements was 2.26%. The designed microdevice was further applied to detect copper ions in practical samples (calf serum sample and synthetic human urine sample) using a standard addition method, and the average recovery was found to be 95–104%. The performance of copper ion detection with the integrated microdevice was consistent with that of the inductively coupled plasma mass spectrometry (ICP-MS) in the same practical samples, demonstrating significant practicality in the test of body fluidics. The portable integrated microdevice is an excellent choice for on-site detection and has a promising prospect in the point-of-care testing (POCT) applications. Graphical abstract
研究领域:
分析化学
·
生物医学工程
·
环境科学
·
即时检测
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Sensors
·
2023
·
IF 8.9
Shixian Liu,Yuting Hou,Zirui Li,Chenyu Yang,Guozhen Liu
A fully integrated device for salivary detection with a sample-in-answer-out fashion is critical for noninvasive point-of-care testing (POCT), especially for the screening of contagious disease infection. Microfluidic paper-based analytical devices (μPADs) have demonstrated their huge potential in POCT due to their low cost and easy adaptation with other components. This study developed a generic POCT platform by integrating a centrifugal microfluidic disc with μPADs to realize sample-to-answer salivary diagnostics. Specifically, a custom centrifugal microfluidic disc integrated with μPADs is fabricated, which demonstrated a high efficiency in saliva treatment. To demonstrate the capability of the integrated device for salivary analysis, the SARS-CoV-2 Nucleocapsid (N) protein, a reliable biomarker for SARS-CoV-2 acute infection, is used as the model analyte. By the chemical treatment of the μPAD surface, and by optimizing the protein immobilization conditions, the on-disc μPADs were able to detect the SARS-CoV-2 N protein down to 10 pg mL–1 with a dynamic range of 10–1000 pg mL–1 and an assay time of 8 min. The integrated device was successfully used for the quantification of the N protein of pseudovirus in saliva with high specificity and demonstrated a comparable performance to the commercial paper lateral flow assay test strips.
研究领域:
微流控技术
·
即时检测
·
诊断学
·
生物医学工程
·
病毒学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Baoqing Zhou,Qinghua Ye,Fan Li,Xinran Xiang,Yuting Shang,Chufang Wang,Yanna Shao,Liang Xue,Jumei Zhang,Juan Wang,Yu Ding,Moutong Chen,Qingping Wu
Rapid, accurate point-of-care testing (POCT) for pathogenic bacteria detection is the key for avoiding foodborne diseases caused by pathogens or their toxins. The lateral flow biosensor (LFB) based on clustered regularly interspaced short palindromic repeats (CRISPR/Cas) has displayed remarkable potential for pathogen diagnosis. In this study, we report a novel CRISPR/Cas12a-based fluorescence enhanced LFB in conjunction with functionalized quantum dots , combined with recombinase-assisted amplification (RAA), to establish low-cost, simple, and sensitive detection of Staphylococcus aureus , namely CRA-LFB (CRISPR/Cas-recombinase-assisted amplification based LFB). The CRA-LFB assay is characterized by Cas12a-mediated trans-cleavage activation induced by the target DNA to digest biotin-DNA probes, caused no complementarity to the capture probe immobilized on the test (T) line, and resulted in an undetectable T line fluorescence signal on LFB. The naked eye or fluorescence strip reader was used to determine the fluorescence intensity of the T and control lines. The limit of detection (at optimal conditions) was as low as 75 aM of genomic DNA, and 5.4 × 10 2 cfu/mL of S. aureus in pure cultures were detected. Moreover, this CRA-LFB assay can rapidly and accurately detect S. aureus in spiked and natural meat and vegetable samples. The CRA-LFB method yielded high specificity and no interference from other nontargeted bacteria. The results exhibited high-resolution, high-intensity fluorescent signals obtained within 70 min. This bacterial detection platform is simple, low cost, almost equipment-free, and fully qualified for food and clinical diagnosis onsite testing requirements.
研究领域:
生物技术
·
微生物学
·
食品安全
·
即时诊断
✓ 已验证
DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2024
·
IF 12.2
Yaxing Ma,Li Chen,Ying Luo,Chuixiu Huang,Xiantao Shen
Lead (Pb) poisoning is estimated to account for 1 % of the global disease burden. The gold standard for diagnosing lead poisoning in human body relies on blood lead level (BLL), which is always performed in hospitals using expensive instruments. However, there are still many countries and regions with a lack of medical resources (without enough professional medical staff and analytical instruments). To achieve a facile diagnosis of lead poisoning by ordinary residents (without any expertise), this study conducted a research study on 810 participants to discover and validate a new lead poisoning indicator (creatinine-corrected urinary lead level, cULL) beyond BLL in non-invasive samples. A point-of-care testing (POCT) device to measure cULL was developed, equipped with liquid-phase microextraction and electromembrane extraction on a paper-based analytical device for on-site separation of lead and creatinine in the urine, using a smartphone for the quantification of analytes. The cULL as a novel indicator and the POCT device developed could be effective in reducing the risk of damage from lead contamination.
研究领域:
环境健康
·
分析化学
·
即时诊断
·
公共卫生
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2024
·
IF 8
Guoqing He,Shukun Zhao,Chen Yang,Longfei Chen,Yantong Liu,Qinghao Hu,Yi Yang
Point-of-care testing (POCT) of chloramphenicol (CAP) in fresh milk from private ranches is essential to ensure food safety. It’s still a great challenge to meet simultaneously rapid and accurate during POCT of CAP. Here, we present a POCT device integrating hydrogel microsphere immunoassay and smartphone Fourier analysis for fast and sensitive detection of CAP. Based on the excellent biocompatibility and outstanding mechanical properties of hydrogel microspheres (100 μm), the antibody activity can be maintained and the mechanical deformation of the hydrogel cross-linking lattice is induced by squeezing to accelerate the antigen-antibody reaction of CAP and anti-CAP antibodies. The signal-to-noise ratio of the detection result is optimized adaptively by a designed microsphere Fourier analysis algorithm for precise CAP concentration detection. The results show that the method decreases incubation time from 60 min to 25 min and meets detection limit requirements of industrial production (0.05 ng/mL, with a dynamic range of 0.05–8 ng/mL). This method not only achieves rapid and accurate on-site detection of CAP in milk but also applies to other low concentrations of harmful substances detection in food field.
研究领域:
食品安全
·
分析化学
·
生物技术
·
材料科学
·
光学
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2023
·
IF 8.4
Xiaodong Lin,Bergoi Ibarlucea,Tao Peng,Ren Shen,Peiyue Li,Pan Zhang
Susceptibility point-of-care testing (POCT) and bacterial inactivation are crucial for food safety and clinical diagnosis since bacteria pose a severe hazard to public health and healthcare. To enable photothermal determination and hyperthermal inactivation of bacteria, vancomycin-modified near-infrared (NIR)-responsive copper selenide nanoparticles (Cu 2−X [email protected] NPs) with excellent photothermal conversion are synthesized here. Cu 2−X [email protected] NPs can be trapped on the surface of Gram-positive bacteria such as Staphylococcus aureus ( S. aureus ), forming a Cu 2−X [email protected] NPs/ S. aureus complex. Suspension (Cu 2−X [email protected] NPs) and sediment (Cu 2−X [email protected] NPs/ S. aureus ) can convert the NIR laser energy into a thermal signal that can be detected by the thermal camera at concentrations as low as 6.0 CUF mL −1 . Meanwhile, S. aureus can be effectively inactivated by localized temperature rises during temperature-based signal detection. The antibacterial efficiency can reach as high as 99.53%. The proposed strategy not only provides a sensitive POCT strategy for bacterial determination but also implements efficient inactivation of bacteria simultaneously.
研究领域:
细菌学
·
纳米技术
·
生物医学工程
·
食品安全
·
临床诊断
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL BIOCHEMISTRY
·
2017
·
IF 2.334
Yuan-Yuan Xue,Wen-Tao Zhang,Meng-Yue Zhang,Li-Zhi Liu,Wen-Xin Zhu,Ling-Zhi Yan,Jing Wang,Yan-Ru Wang,Jian-Long Wang,Dao-Hong Zhang
Microfluidic paper-based analytical devices (μPADs) have a significant potential in developing portable and disposable point-of-care testing (POCT). Herein, a facile, rapid, cost-effective and environment friendly strategy for μPADs fabrication is proposed. Specifically, the substrate paper was hydrophobized by coating with trimethoxysilane (TOS), and then the selected area was hydrophilized by treating with surfactant. The whole fabrication process was implemented within 7 min, with no need for complex pre-treatment, high-temperature and special equipment. As a proof-of-concept application, the as-prepared μPAD was applied to determination of the glucose content in human serum samples. The results agreed well with those obtained by a glucometer. We believe that the μPADs fabrication method proposed here could provide a facile, rapid and low-cost reference for other related studies.
研究领域:
分析化学
·
生物医学工程
·
即时诊断
✓ 已验证
DOI
PubMed
高影响因子
ELECTROANALYSIS
·
2021
·Vol. 34
·期 2
·345-351
·
IF 3.223
Xiaofang Zhang,Jing Chen,Qia Wang,Bing Du,Gaochao Fan,Weidong Zheng,Haipeng Yang,Tailin Xu
Sarcosine level in serum is of important clinical significance in distinguishing prostate cancer. This work depicts an amperometric sarcosine biosensor with good anti-interference performance by electro-codepositing manganese phosphate, 3,4-ethylenedioxythiophene (EDOT) and indole-6-carboxylic acid (IA) on the glass carbon electrode. The prepared sarcosine biosensor has a wide linear detection range (1–55 μM) with a low detection limit of 0.11 μM. This work provides an anti-interference approach by controlling the surface charge density of the biosensor to sarcosine sensing, which has great potential to be used as point of care testing (POCT) device for the rapid detection of prostate cancer biomarkers.
研究领域:
生物医学工程
·
分析化学
·
癌症研究
✓ 已验证
产品关联
DOI
高影响因子
ACS Sensors
·
2021
·
IF 7.711
Lei Cao,Xiaojin Guo,Ping Mao,Yulin Ren,Zedong Li,Minli You,Jie Hu,Miao Tian,Chunyan Yao,Fei Li,Feng Xu
Digital polymerase chain reaction (dPCR) has found widespread applications in molecular diagnosis of various diseases owing to its sensitive single-molecule detection capability. However, the existing dPCR platforms rely on the auxiliary procedure to disperse DNA samples, which needs complicated operation, expensive apparatus, and consumables. Besides, the complex and costly dPCR readers also impede the applications of dPCR for point-of-care testing (POCT). Herein, we developed a portable digital loop-mediated isothermal amplification (dLAMP) platform, integrating a microscale hydrogel (microgel) array chip for sample partition, a miniaturized heater for DNA amplification, and a hand-held reader for digital readout. In the platform, the chip with thousands of isolated microgels holds the capability of self-absorption and partition of DNA samples, thus avoiding auxiliary equipment and professional personnel operations. Using the integrated dLAMP platform, λDNA templates have been quantified with a good linear detection range of 2–1000 copies/μL and a detection limit of 1 copy/μL. As a demonstration, the epidermal growth factor receptor L858R gene mutation, a crucial factor for the susceptibility of the tyrosine kinase inhibitor in non-small-cell lung cancer treatment, has been accurately identified by the dLAMP platform with a spiked plasma sample. This work shows that the developed dLAMP platform provides a low-cost, facile, and user-friendly solution for the absolute quantification of DNA, showing great potential for the POCT of nucleic acids.
研究领域:
分子诊断
·
生物医学工程
·
癌症研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）