EUROPEAN POLYMER JOURNAL
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2023
·
IF 6
Yina Zhuge,Fujuan Liu
In order to obtain fiber membrane materials with excellent thermal insulation properties, we successfully prepared lightweight PI/PSA porous micro-nano fiber membranes by microemulsion electrospinning technology combined with calcination in this work. The morphology, tensile strength and thermal insulation properties of the fiber membranes were fully characterized. The PI/PSA micro-nano fiber membranes prepared at the PI concentration of 17 wt%, the dispersed phase/continuous phase mass ratio of 1/10, the PI/PSA mass ratio of 7/3, and the voltage of 16 kV exhibited the best morphology and the most uniform fiber diameter. Besides, the PI/PSA porous micro-nano fiber membrane obtained after calcination had excellent tensile properties (breaking stress: 10.54 ± 1.96 MPa) and thermal insulation performance (thermal conductivity: 44.7 mW·m −1 ·K −1 ). The thermal imaging and thermocouple results showed that the thermal insulation performance of PI/PSA porous micro-nano fiber membrane was superior to that of PI/PSA membrane, and the thermal insulation properties became more excellent as the number of membrane layers increased. The PI/PSA porous micro-nano fiber membrane with low thermal conductivity acquired in this work is expected to broaden the application of protective clothing.
研究领域:
材料科学
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热能工程
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产品关联
DOI
高影响因子
Advanced Science
·
2023
·Vol. 10
·期 15
·2206494
·
IF 15.1
Shaowei Xie,Xiaochen Fei,Jiayi Wang,Yi-Cheng Zhu,Jiazhou Liu,Xinxing Du,Xuesong Liu,Liang Dong,Yinjie Zhu,Jiahua Pan,Baijun Dong,Jianjun Sha,Yu Luo,Wenshe Sun,Wei Xue
High-throughput metabolic fingerprinting has been deemed one of the most promising strategies for addressing the high false positive rate of prostate cancer (PCa) diagnosis in the prostate-specific antigen (PSA) gray zone. However, the current metabolic fingerprinting remains challenging in achieving high-precision metabolite detection in complex biological samples (e.g., serum and urine). Herein, a novel self-assembly MoS 2 /MXene heterostructure nanocomposite with a tailored doping ratio of 10% is presented as a matrix for laser desorption ionization mass spectrometry analysis in clinical biosamples. Notably, owing to the two-dimensional architecture and doping effect, MoS 2 /MXene demonstrates favorable laser desorption ionization performance with low adsorption energy, which is evidenced by efficient urinary metabolic fingerprinting with an enhanced area under curve (AUC) diagnosis capability of 0.959 relative to that of serum metabolic fingerprinting (AUC = 0.902) for the diagnosis of PCa in the PSA gray zone. Thus, this MoS 2 /MXene heterostructure is anticipated to offer a novel strategy to precisely and noninvasively diagnose PCa in the PSA gray zone.
研究领域:
医学诊断
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材料科学
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生物化学
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DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2020
·Vol. 187
·期 11
·1-10
·
IF 6.232
Chen Wenqin,Li Jia,Wei Xiaotong,Fan Yunpeng,Qian Husun,Li Siqiao,Xiang Yu,Ding Shijia
Based on the hydrogel-AuNP supramolecular sphere (H-Au), a label-free and real-time surface plasmon resonance imaging biosensor has been developed for highly sensitive and specific determination of prostate cancer cell-derived exosomes. After integrating the signal amplification effect of the mass cumulative hydrogel and the LSPR effect of AuNPs with high specific aptamer, the SPRi biosensor for exosome detection exhibited a wide linear range from 1.00 × 10 5 to 1.00 × 10 7 particles/mL with a limit of detection of 1.00 × 10 5 particles/mL. Most importantly, with a strong correlation between the SPRi signal and the t-PSA value measured by the clinical chemiluminescence immunoassay, this biosensor displayed excellent practicability for human serum analysis, which exhibits great potential applications in disease diagnosis and bioanalysis. Graphical abstract
Prostate cancer has been one of the most threatening diseases in human life and health nowadays. In particular, as cancer metastasizes, it is more likely to cause fracture, paraplegia, and even fatal consequences. However, the predominant t-PSA test needs further improvement for the deficiencies of limited specificity and sensitivity, which is prone to false positive. As one of the noninvasive markers of liquid biopsies, exosome has the potential to be a substitute for t-PSA, which can provide specific and predictive information in disease diagnosis and prognosis. Herein, based on the hydrogel-AuNP supramolecular sphere (H-Au), a label-free and real-time surface plasmon resonance biosensor has been developed for highly sensitive and specific detection of prostate cancer cell-derived exosomes. After integrating the signal amplification effect of mass cumulative hydrogel and LSPR effect of AuNPs with high specific aptamer, this developed SPRi biosensor for exosome detection exhibited a wide linear range from 1.00 × 10 5 to 1.00 × 10 7 particles/mL with a limit of detection down to 1.00 × 10 5 particles/mL. Most importantly, with a strong correlation between the SPRi signal and the t-PSA value measured by the clinical chemiluminescence immunoassay, this biosensor displayed excellent practicability in human serum, which exhibited great potential applications in disease diagnosis and bioanalysis.
研究领域:
生物传感器技术
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癌症诊断
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纳米医学
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2017
·
IF 5.401
Bi-Yun Fang,Chun-Yuan Wang,Cheng Li,Hai-Bo Wang,Yuan-Di Zhao
In this paper, we proposed an amplified assay using DNase I for sensitively sensing prostate specific antigen (PSA) based on CdSe/ZnS quantum dot labelled PSA aptamer/graphene oxide (QD-aptamer/GO). In this sensing system, GO could strongly bind QD conjugated aptamer and quench the fluorescence of QD, furthermore, GO could protect DNA from nuclease cleavage. When binding target PSA, aptamer probe released from the surface of GO nanosheet, then the free aptamer could be cleaved by nuclease, thus the QD and PSA were liberated, after that, the released PSA would bind another aptamer on GO nanosheet and start a new cycle, which resulted in amplification of restoring signal. The probe possessed steady fluorescence signal and high specificity due to the anti photobleaching of QD and the high affinity of aptamer to PSA. Under the optimum experimental conditions, fluorescence intensity increased linearly with the PSA concentration between 0.1 fg mL −1 and 3 fg mL −1 with the limit of quantification of 0.05 fg mL −1 , which was three orders of magnitude lower than that without DNase I. Finally, the method was applied to quantification of PSA in human serum samples.
研究领域:
生物传感器
·
分析化学
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纳米技术
·
癌症诊断
✓ 已验证
DOI
高影响因子
MICROCHIMICA ACTA
·
2016
·Vol. 183
·期 8
·2385-2393
·
IF 4.831
Sun Xue-cheng,Lei Chong,Guo Lei,Zhou Yong
We describe a micro fluxgate based device with rectangular magnetic core for the determination of prostate specific antigen (PSA) labeled with Dynabeads. A sandwich immunoassay was employed where PSA is captured on a gold film modified with a self-assembled monolayer of antibody. The secondary antibody is labeled with Dynabeads. By applying a DC magnetic fields in the range of 460 to 700 μT, PSA can be detected with detection limit as low as 0.1 ng mL −1 . This micro fluxgate-based assay offers the advantages of miniaturization, simple and conveniently manipulation, re-usability and stability. In our perception, it offers a viable approach towards clinical determination of PSA or other biomarkers.
A separable detection method based on micro fluxgate and immunomagnetic beads was developed for detection of prostate specific antigen (PSA). Sandwich immunoassay was employed where PSA is captured on a gold film modified with a self-assembled monolayer of antibody. By applying a DC magnetic fields in the range of 460 to 700 μT, PSA can be detected with detection limit as low as 0.1 ng mL −1 , and this bio-sensing system can also give an approximate quantitation to the concentrations of them.
研究领域:
生物传感器
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医学诊断
·
分子生物学
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产品关联
DOI
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2018
·
IF 3.997
Bi-Yun Fang,Jie An,Bo Liu,Yuan-Di Zhao
In this work, a label-free Ag nanocluster (AgNC)-based fluorescent probe is proposed to detect tumor marker, prostate-specific antigen (PSA). In the experiments, DNA sequences containing segments complemented to different parts of PSA aptamer were used to synthesize DNA-Ag nanoclusters (DNA-AgNC). Some of the obtained specific DNA-AgNC exhibited significant fluorescence increase after hybridization with PSA aptamer. Based on this, a simple DNA-AgNC/aptamer hybridization probe was fabricated for PSA detection using fluorescence quenching, because competitively specific binding between PSA and its aptamer inhibited the fluorescence enhancement effect of PSA aptamer on DNA-AgNC. The sequence of template DNA, pH and salt concentration of binding buffer, and the concentration of aptamer were optimized. Under optimum conditions, the concentration of PSA within the range of 2–150 ng mL −1 with the detection limit of 1.14 ng mL −1 was detected (3σ; n = 7). This approach was also successfully applied to determine PSA in spiked serum samples. As is well known, this was the first report to realize PSA detection using fluorescent AgNC-based probe. This work would provide reference for construction of AgNC-based probes for detecting other proteins.
研究领域:
生物化学
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分析化学
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纳米技术
·
生物传感器
✓ 已验证
产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2024
·
IF 4.9
Yina Zhuge,Haipei Ge,Fujuan Liu
The white-browed penguin can survive in extreme environments due to its unique feather structure. The feather shafts of penguins are porous, separated by the membrane-pore-like structure made of keratin fiber membranes. The feather branches are densely arranged around the feather shafts. This unique structure provides inspiration for researchers. In this work, we developed a simple method to construct polyimide/polysulfone amide (PI/PSA) membrane-pore-like structure nanofibers resembling penguin feather shafts and studied the effects of different low-boiling point solvents (tetrahydrofuran (THF), ethyl acetate (EA), and acetone (AC)) on the morphology, tensile properties, and thermal insulation properties of PI/PSA membrane-pore-like structure nanofibers. Comprehensive characterization was also performed using scanning electron microscope, total reflection infrared spectrometer, X-ray diffractometer, universal testing machine, thermal conductivity meter, pore size analyzer, thermal gravimetric analyzer, infrared thermal imaging instruments, and thermocouple testing instruments. The results showed that when the mass ratio of THF to N, N-dimethylacetamide (DMAC) was 20/80, the prepared PI/PSA membrane-pore-like structure nanofibers exhibited excellent thermal insulation properties, with a low thermal conductivity of 0.0436 W·m −1 ·K −1 , a breaking strength of 7.72 ± 2.64 MPa, and a breaking elongation of 8.19 ± 4.07 %. The biomimetic membrane-pore-like structure with excellent thermal insulation properties is expected to be applied in various fields, such as heat-protective clothing and architectural coatings in harsh environments.
研究领域:
材料科学
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仿生学
·
热物理学
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产品关联
DOI
高影响因子
PROGRESS IN ORGANIC COATINGS
·
2021
·
IF 5.161
Bingyan Sun,Huimin Wang,Yanchao Fan,Xiaomeng Chu,Shaojie Liu,Song Zhao,Minzhong Zhao
For UV-induced peelable pressure sensitive adhesive (PSA) used in the wafer dicing process, improving the peel performance can effectively increase the process reliability and the yield of subsequent packaging process. In this work, a NCO-containing castor oil-based urethane acrylate oligomer called CO -PUB with dual curable functions (UV-induced polymerization and NCO/OH reaction) was obtained by partially sealing a castor oil-based urethane oligomer with hydroxyethyl acrylate. Fourier transform infrared spectroscopy (FTIR) and nuclear magnetic resonance spectrometer (NMR) results indicated that CO-PUB was successfully prepared. CO-PUB, OH-containing acrylic copolymer , multifunctional acrylate monomer and photo-initiator were used to construct a UV-induced peelable adhesive for silicon wafer dicing. The results showed that NCO groups in CO-PUB underwent the NCO/OH reaction with the acrylic copolymer to obtain a vinyl pendant cross-linked acrylic copolymer. The peel strength of this peelable adhesive before UV irradiation could exceed 20 N/25 mm. After UV irradiation, the unsaturated vinyl bonds contained in it were copolymerized with the multifunctional acrylate monomer to form a fully cross-linked adhesive film and the peel strength could be reduced to below 1 N/25 mm. Compared with UV-induced peelable adhesives prepared by other reported curing agents, the adhesive prepared from CO-PUB reduced UV dose required for curing. The cross-linking degree of cured adhesive film was also increased and the gel content was up to 95%. The residual adhesive after peeling off could be reduced to about 0.32%. In addition, CO -PUB used renewable castor oil as polyol and did not need to add any organic solvent during the preparation process, providing a new idea for the green preparation of UV-induced peelable adhesive with excellent performances.
研究领域:
材料科学
·
化学工程
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产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2020
·Vol. 187
·期 6
·1-9
·
IF 6.232
Li Xiaomeng,Kong Weisu,Qin Xia,Qu Fengli,Lu Limin
A facile and sensitive self-powered cathodic photoelectrochemical (PEC) aptasensor is reported for the detection of prostate-specific antigen (PSA) based on CuO-Cu 2 O nanowire array grown on Cu mesh (CuO-Cu 2 O NWA/CM) as electrode. The mixed narrow band gaps of the CuO-Cu 2 O heterostructure ensured its wide absorption band, effective electron/hole separation, and high photocatalytic activity in the visible region. In addition, nanowires directly grown on the substrate provided high specific surface area and exposed abundant active sites, thus guaranteeing its high photocatalytic efficiency. Therefore, the self-powered sensor exhibited favorable analytical performance with fast response, wide linear ranges of 0.01 to 5 ng/mL and 5 to 100 ng/mL, an acceptable detection limit of 3 pg/mL, and reasonable selectivity and stability. The proposed CuO-Cu 2 O NWA/CM can be considered a promising visible light-responsive photoactive material for fabrication of PEC aptasensor with high performance.
a Schematic illustration of construction process of PEC sensing platform based on the CuO-Cu 2 O composite for PSA detection. b Schematic mechanism of the operating PEC system.
研究领域:
分析化学
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生物传感器
·
纳米材料
·
癌症诊断
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF APPLIED POLYMER SCIENCE
·
2015
·Vol. 133
·期 15
·
IF 1.768
Jun Hua Chen,Qiang Zhao,Ying Wang,Fubin Luo,Lu Shen,Kun Wu,Li Yan Liang,ManGeng Lu
A heat-resistance monomer denoted as triazine heterocyclic compound (TGIC-AA) was synthesized and applied into improving the thermal stability of solvent-based acrylic pressure sensitive adhesives (PSAs) through copolymerization. The modified acrylic PSAs tapes possessed longer holding time at temperature up to 150°C and no large areas of residues could be seen when peeled off on the substrate while the temperature of test was cooled down to room temperature. The thermal stability could be significantly enhanced in PSAs as the content of triazine heterocyclic compounds increased due to the extensive crosslinking networks. This indicated a worthy method to prepared heat resistant acrylic PSAs. An obvious reduction in peel adhesion occurred at the content of crosslinkers range 5 wt %-7 wt %, while beyond 7 wt % adhesion failure occurred. The influences of crosslinking density on the molecular weight, glass transition temperature and viscosity, etc. for PSAs were also studied. © 2015 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2016 , 133 , 43281.
研究领域:
高分子科学
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材料科学
·
胶黏技术
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产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2020
·Vol. 187
·期 2
·1-7
·
IF 6.232
Sun Ting,Xia Ning,Yuan Fang,Liu Xiaoman,Chang Yong,Liu Shudi,Liu Lin
Biotinylated peptide-Cu 2+ nanoparticles (Cu-P NPs) were synthesized via “one-pot” self-assembly. The peptide P conststs of a hydrophobic dipeptide (FF), a tripeptide (KGH), and a biotin moiety attached to the terminal amino group of the Lys residue. The Cu-P NPs contain abundant catalytically active Cu 2+ ions which are liberated by acid-induced dissolution. The released Cu 2+ ions catalyze the oxidization of 3,3′,5,5′-tetramethylbenzidine (TMB) by H 2 O 2 because of their intrinsic peroxidase activity, and this results in the formation of a blue-green coloration. Based on the streptavidin–biotin interaction, the Cu-P NPs were employed to establish an enzyme-free colorimetric method for determination of prostate-specific antigen (PSA) as a model biomarker. Under optimal conditions, the linear response range is 0.001–1 ng mL −1 , with a limit of detection as low as 1 pg mL −1 .
Schematic illustration of a colorimetric immunoassay for the prostate specific antigen (PSA) with biotinylated peptide-Cu 2+ nanoparticle (Cu-P NP) as the signal label based on the streptavidin (SA)–biotin interaction. The signal was produced by Cu 2+ -catalyzed oxidization of 3,3′,5,5′-tetramethylbenzidine (TMB). P: KGHFF
研究领域:
分析化学
·
生物标志物检测
·
纳米技术
·
癌症诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF NANOPARTICLE RESEARCH
·
2022
·Vol. 24
·期 5
·1-10
·
IF 2.533
Chen Junjie,Li Xiangqian,Yu Xiaoqi,Zhou Wei,Wang Qianming
Two-dimensional platforms generate considerable interests due to diverse application. We report a “turn-on” fluorescent nanoprobe for prostate-specific antigen (PSA) detection. It is based on cobalt oxyhydroxide nanosheets (CoOOH NSs). Fluorescein amidite (abbreviated as FAM)-labeled aptamer probe (abbreviated as FAM-aptamer) has been adsorbed onto the nanosheets. Energy transfer between substrate and optical species has induced the quenching of FAM-aptamer emission. The strong affinity of FAM-aptamer to the target PSA causes the formation of a rigid aptamer structure. The integration between FAM-aptamer and CoOOH NSs has been drastically affected. The release of the aptamer probe from the nanosheet surface has been realized. The green luminescence has been recovered. The dynamic nanosensor exhibits highly sensitive performance toward PSA with a linear detection range from 0.1 to 5 ng/mL. Its detection limit is measured to be 56.1 pg/mL. Therefore, a simple and efficient sensing platform for the detection of prostate cancer can be established.
研究领域:
生物纳米技术
·
分析化学
·
癌症诊断
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）