Fishes
·
2025
·Vol. 10
·期 3
·92
·
IF 2.4
Yijun Jia,Zhao Zhao,Leyang Chen,Yongqi Liu,Bin Zhu
A number of viral diseases have significantly impeded the growth of the aquaculture industry. Antiviral drugs represent an effective means of controlling infection. However, the efficacy of the entire therapeutic process is contingent upon the availability of an efficient delivery system. This study selected three common antiviral drugs and constructed corresponding drug delivery systems utilising single-walled carbon nanotubes (SWCNTs) as carriers. The reliability of carbon nanotubes as delivery carriers was evaluated by detecting the therapeutic effect on infectious splenic and renal necrosis virus (ISKNV). The findings demonstrated that SWCNTs can effectively enhance the absorption of the three drugs into the body, prolong their metabolic half-life, and improve the survival rate of fish infected with ISKNV. The Ribavirin-SWCNTs (RBV-SWCNTs) group exhibited the most pronounced protective effect, with a mortality rate of less than 25%. It was observed that SWCNTs facilitated the rapid transportation of ribavirin, with the drug content in the RBV-SWCNTs group being approximately double that of the free ribavirin group. Furthermore, this system markedly diminished the viral load, augmented enzyme activities, and elevated antiviral gene expression. This study indicated that carbon nanotubes are optimal carriers for antiviral drugs, which have considerable potential as a delivery vehicle for antiviral drugs to prevent viral infections in aquaculture.
研究领域:
水产养殖学
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病毒学
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药物递送
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2024
·
IF 5.7
Yucan Zhang,Chenxi Shi,Jin Yuan,Yue Zhang,Xiaohui Jin,Shaopo Zu,Honglei Zhang,Hui Hu
Porcine deltacoronavirus (PDCoV) is an emerging enteropathogenic coronavirus that mainly causes diarrhea and death in suckling piglets and also has the potential for cross-species transmission, threatening public health. However, there is still no effective vaccine or drug to prevent PDCoV infection. In order to accelerate the development of antiviral drugs, we established a high-throughput screening platform using a novel genome editing technology called transformation-associated recombination cloning in yeast. The recombinant PDCoV and PDCoV reporter virus expressing enhanced green fluorescent protein were both rapidly rescued with stable genealogical characteristics during passage. Further study demonstrated that the reporter virus can be used for high-throughput screening of antiviral drugs with a Z-factor of 0.821–0.826. Then, a medicine food homology compound library was applied, and we found that three compounds were potential antiviral reagents. In summary, we have established a fast and efficient reverse genetic system of PDCoV, providing a powerful platform for the research of antiviral drugs.
研究领域:
病毒学
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分子生物学
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药理学
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生物技术
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PubMed
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Frontiers in Nutrition
·
2025
·
IF 5.1
Zhanding Cui,Jinlong Liu,Jinlong Wang,Jing Zhang,Yimei Cao,Kun Li,Zhixun Zhao,Hong Yuan,Xingwen Bai,Xueqing Ma,Pinghua Li,Yuanfang Fu,Huifang Bao,Dong Li,Qiang Zhang,Zaixin Liu,Kai Li,Tong Xu,Zengjun Lu
This study delves into the antiviral efficacy of Formononetin (FMN) and Mizoribine (MZR) against the Porcine Reproductive and Respiratory Syndrome Virus (PRRSV), a virus with a considerable economic impact and a current void in effective treatments. FMN and MZR were found to inhibit various PRRSV strains in vitro, predominantly in the early stages of viral infection. Noteworthy was the observation of their synergistic effects when combined with Ribavirin. The study underscores the antiviral potential of FMN and MZR, particularly emphasizing their low cytotoxicity at specific concentrations. These results position FMN and MZR as promising antiviral agents against PRRSV, underscoring their low cytotoxicity and efficacy in early-stage viral inhibition. Such findings pave the way for their potential inclusion in future PRRSV management strategies.
研究领域:
兽医学
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病毒学
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药理学
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PubMed
高影响因子
WATER RESEARCH
·
2024
·
IF 11.4
Haichuan Zhang,Jiawei Li,Shanshan Ye,Xiyang Zou,Rongxin Fei,Xinyu Hu,Ji Li
The electro-peroxone (EP) process encounters two inherent challenges in wastewater treatment: sluggish O 2 /O 3 transfer and substantial ozone waste. To overcome these limitations, we introduced micro-nano bubbles (MNBs) aeration to enhance O 2 /O 3 dissolution and diffusion, ultimately aiming to improve the removal of trace pharmaceutical contaminants from hospital wastewater. In the MNBs aeration system, the ozone transfer coefficient ranging from 0.536 to 0.265 min −1 , significantly surpassing that of conventional aeration (0.220 to 0.090 min −1 ) by approximately 2 to 4.5 times. Consequently, the EP process under MNBs aeration significantly enhanced ozone-resistant ibuprofen (IBU) removal, achieving a removal rate of 98.4 ± 1.5 %, far exceeding the 47.3 ± 4.7 % observed with conventional aeration. This significant improvement was attributed to the heightened production of hydroxyl radicals (•OH), reaching 0.97 × 10 −9 M s, compared to only 0.28 × 10 −9 M s in conventional aeration. The mechanism behind the enhanced •OH production in the MNBs-EP process relied primarily on two factors: improved O 2 /O 3 dissolution due to high internal pressure/large surface and enhanced O 3 /H 2 O 2 activation from high collapse energy. These factors together contributed to the robust oxidation capability of the MNBs-EP system. As a result, over 97 % removal efficiency was achieved for five representative pharmaceutical pollutants (sulfamethoxazole, ribavirin, norfloxacin, tetracycline and ampicillin) in just 1 min. Furthermore, when applied to real hospital wastewater, the MNBs-O 3 -E treatment system reduced all 15 detected trace pharmaceutical compounds to below 10 ng L −1 and achieved 14 types of pollutants with removal rates of over 85 % within 15 min, resulting in an ultrahigh total removal rate of 98.6 %, from an initial total concentration of 2108 ng L −1 to less than 30 ng L −1 . Thus, micro-nano aeration endowed the EP process as a promising advanced oxidation system for rapid and highly-effective removal of trace pharmaceutical contaminants from hospital wastewater.
研究领域:
环境科学
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水处理技术
·
高级氧化工艺
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2024
·
IF 5.7
Zilin Wu,Min Huang,Junmei Jiang,Chun Zhang,Gefei Hao,Moxian Chen,Qing X. Li,Mengao Jia,Junfeng Liu,Xiangyang Li
Ningnanmycin has been proven to effectively inhibit infection by potato virus Y (PVY), although its underlying mechanisms remain unclear. In this study, we report a novel finding that ningnanmycin affects PVY replication. Two approaches were employed: studies using PVY movement-deficient mutants suggest that ningnanmycin, at a concentration of 500 μg/mL, inhibits PVY replication. Ningnanmycin interacts with the PVY-encoded coat protein (CP) with a binding constant of 1.34 μmol/L, and key amino acids Glu168 and Thr206 are involved in this interaction. Additionally, ningnanmycin induces the expression of antiviral response genes in Nicotiana benthamiana, including PRXIIB, PRXIIE, PUB4, and PER42. Furthermore, studies revealed that the overexpression of PUB4 in N. benthamiana confers resistance to PVY infection. These findings highlight the mechanisms by which ningnanmycin activates the PUB4 gene and suppresses CP assembly, thereby inhibiting PVY in N. benthamiana. This study represents an important step toward elucidating the molecular mechanism underlying the antiviral activity of ningnanmycin.
研究领域:
植物病理学
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病毒学
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分子生物学
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植物生物技术
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Nature Communications
·
2024
·Vol. 15
·期 1
·1-12
·
IF 14.7
Cui Zhanding,Liu Jinlong,Xie Chong,Wang Tao,Sun Pu,Wang Jinlong,Li Jiaoyang,Li Guoxiu,Qiu Jicheng,Zhang Ying,Li Dengliang,Sun Ying,Yin Juanbin,Li Kun,Zhao Zhixun,Yuan Hong,Bai Xingwen,Ma Xueqing,Li Pinghua,Fu Yuanfang,Bao Huifang,Li Dong,Zhang Qiang,Liu Zaixin,Cao Yimei,Zhang Jing,Lu Zengjun
Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) poses a major threat to the global swine industry, yet effective prevention and control measures remain elusive. This study unveils Nitazoxanide (NTZ) as a potent inhibitor of PRRSV both in vitro and in vivo. Through High-Throughput Screening techniques, 16 potential anti-PRRSV compounds are identified from a library comprising FDA-approved and pharmacopeial drugs. We show that NTZ displays strong efficacy in reducing PRRSV proliferation and transmission in a swine model, alleviating viremia and lung damage. Additionally, Tizoxanide (TIZ), the primary metabolite of NTZ, has been identified as a facilitator of NMRAL1 dimerization. This finding potentially sheds light on the underlying mechanism contributing to TIZ’s role in augmenting the sensitivity of the IFN-β pathway. These results indicate the promising potential of NTZ as a repurposed therapeutic agent for Porcine Reproductive and Respiratory Syndrome (PRRS). Additionally, they provide valuable insights into the antiviral mechanisms underlying NTZ’s effectiveness.
研究领域:
兽医学
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分子生物学
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药理学
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病毒学
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治疗学
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PubMed
高影响因子
ACS ES&T Water
·
2026
·
IF 4.3
Zijun Dong,Zhihao Han,Lulu Zhang,Xiaohui Sun,Chenyu Wang,Yifeng Yang
The pervasive use of antiviral drugs (ATVs) for treating viral infections (e.g., HIV, hepatitis, and influenza) has led to their widespread detection in aquatic environments. However, their incomplete removal in wastewater treatment plants (WWTPs) and associated ecological risks remain poorly quantified, particularly in megacities with high population density and medical facility density post-COVID-19. This study analyzed influent, effluent, and process-unit samples from 16 WWTPs and surface water samples from two major river basins via UPLC–ESI–MS/MS to systematically evaluate the occurrence, removal dynamics, and ecological risks of eight priority ATVs. Ecological risk quotients (RQs) were calculated using measured environmental concentrations (MECs) and predicted no-effect concentrations (PNECs) for aquatic organisms. Acyclovir (ACV) was ubiquitously detected in all WWTPs (197–21,334 ng/L) and rivers (139–431 ng/L), with WWTP removal rates varying drastically by process (15.6–99.9%). Oseltamivir (OS) dominated influents (18,251–21,334 ng/L) but was absent in surface waters, suggesting rapid environmental transformation. Risk assessment identified high-risk RQs (>1) for ACV and OS in effluents, threatening algae and fish. Hybrid processes (e.g., A2O+MBR) outperformed single-stage systems, yet overall ATV removal remained suboptimal (avg. 48.6%). This work underscores the urgent need for process optimization and regulatory frameworks to mitigate ATV-driven ecological hazards.
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NANO
·
2026
·
IF 1.3
Haoda Zhang,Kai Huang,Sen Zhou,Xiaoming Yang
Excessively employing tetracycline (TC) or even abusing has led to severe environmental pollution and health risks, and monitoring its amount and related residue is of great importance. Although fluorescent carbon dots (CDs) have been employed to detect TC, single-fluorescence detection usually shows the deficiencies of the limited interference resistance and relatively narrow detection range. This study introduced a dual-channel method based on nitrogen-doped phosphorescent carbon dots (N-CDs). By capitalizing on the synergistic effects of fluorescence and phosphorescence signals, this approach effectively improved the detection sensitivity and the linear range. These N-CDs were synthesized through a one-step microwave-assisted method using guanidinopropionic acid and amidinothiourea as the precursors. The findings revealed that TC selectively quenched both the fluorescent and phosphorescent signals of N-CDs mainly based on the inner filter effect (IFE). These findings not only validated the theoretical basis of the dual-channel detection approach but also highlighted its practical potential. The developed method provided a sensitive and reliable technique for detecting TC in various samples and demonstrated the versatility of N-CDs as promising sensing materials.
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PHARMAZIE
·
2021
·Vol. 76
·期 5
·195-201
·
IF 1.267
Dai Tianming,Wang Min,Wang Pengzhen,Dai Libing,Dai Renke,Meng Qingqi
The effects of eight oral anti-coronavirus drugs (lopinavir, ritonavir, chloroquine, darunavir, ribavirin, arbidol, favipiravir, oseltamivir) on the metabolism of four specific glycosides (polydatin, geniposide, quercitrin, glycyrrhizin) and on the activities of three major glycosidases
(β-glucosidase, α-rhamnosidase, β-glucuronidase) from gut microflora were explored in vitro and determined by LC-MS/MS. The metabolism of polydatin, geniposide, quercitrin and glycyrrhizin was significantly inhibited by one or several anti-coronavirus drugs of 100 μM
around 1 h and 4 h (P<0.05), among which darunavir could strongly reduce the production of genipin (70.6% reduction), quercitin (80.6% reduction) and glycyrrhetinic acid (37.9% reduction), which may cause a high risk of herb-drug interactions (HDI). Additionally, chloroquine reduced the
production of genipin and quercitin by more than 75% (P<0.05), whereas arbidol had no significant influence on the metabolism of polydatin, quercitrin and glycyrrhizin (P>0.05) so that its risk may be lower. The inhibition of darunavir on β-glucosidase was relatively strong (IC50
= 193±23 μM), and the inhibition became weaker on β-glucuronidase and α-rhamnosidase (IC50>500 μM). The consistency between gut microflora and glycosidase system indicated that the inhibition of darunavir on the activity of β-glucosidase and β-glucuronidase
may be the main reason for affecting the metabolism of geniposide, glycyrrhizin and polydatin in gut microflora. However, for the inhibition of darunavir and chloroquine on the metabolism of quercetrin, there was no correlation between gut microflora and α-rhamnosidase system. Assessing
the risk of HDI mediated by glycosidases in gut microflora may be conducive to the safety and efficacy of combining traditional herbal and Western medicine for the treatment of patients with Covid-19.
研究领域:
药理学
·
微生物学
·
生物化学
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PubMed
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2021
·Vol. 413
·期 19
·4855-4863
·
IF 4.157
Ren Le,Qi Shuo,Khan Imran Mahmood,Wu Shijia,Duan Nuo,Wang Zhouping
Acyclic guanosine analogues, a class of widely used antiviral drugs, can cause chronic toxicity and virus resistance. Therefore, it is essential to establish rapid and accurate methods to detect acyclic guanosine analogues. In this study, five acyclic guanosine analogues (acyclovir, famciclovir, ganciclovir, penciclovir, and valaciclovir) were used as positive targets to obtain broad-spectrum aptamers through Capture-SELEX technology. Real-time quantitative PCR (Q-PCR) was used to monitor the aptamer SELEX process. After the sixteen rounds of selection against mixed targets, sequences were obtained by high-throughput sequencing (HTS). Furthermore, a broad-spectrum aptamer, named CIV6, was found as the higher performance aptamer that was suitable for five acyclic guanosine analogues by graphene oxide (GO) polarization and fluorescence assay. Finally, the aptamer CIV6 was used to construct GO fluorescence assay to detect five acyclic guanosine analogues. The limits of detection (LOD) of acyclovir, famciclovir, ganciclovir, penciclovir, and valaciclovir were 0.48 ng·mL −1 , 0.53 ng·mL −1 , 0.50 ng·mL −1 , 0.56 ng·mL −1 , and 0.38 ng·mL −1 , respectively. Graphical abstract
研究领域:
分子生物学
·
分析化学
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药理学
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PubMed
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JOURNAL OF VIROLOGICAL METHODS
·
2021
·
IF 2.014
Yunlong Yan,Maoxi Huang,Li Wang,Wei Xue,Xin Xie,Xiangyang Li
Cucumber mosaic virus (CMV) is a detrimental plant virus in agricultural production. Traditionally, the half-leaf method using Nicotiana glutinosa has been used for screening agrochemicals to control CMV. However, this forms a time-consuming experimental bottleneck. In this study, we constructed a rapid screening model for anti-CMV compounds using CMV. In short, purified CMV particles were labeled through amine reactions and then subjected to binding studies with commercial compounds. The relative gene expression levels were then confirmed. Additionally, the rapid screening model results were verified using synthesized compounds. The commercial compounds ningnanmycin, ribavirin, and moroxydine hydrochloride bound to CMV with dissociation constants of 0.012, 2.870, and 0.069 μM, respectively, and they significantly inhibited expression of the gene for the CMV coat protein in CMV-infected tobacco leaves. This rapid screening model was assessed using our synthetic compounds N12 , N16 , and N18 through binding, which were shown to have dissociation constants 0.008, 0.025, and 70.800 μM, respectively, as well as via gene expression studies. Thus, a rapid method for screening anti-CMV commercial compounds and our synthetic compounds was constructed and confirmed.
研究领域:
农业科学
·
植物病理学
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药理学
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SENSORS AND ACTUATORS B-CHEMICAL
·
2020
·
IF 7.1
Xiaoming Ma,Shan He,Ying Zhang,Jianguo Xu,Huifang Zhang,Zhen Wang,Jun Xue,Xun Li,Wenbo Yu,Xiaolin Fan
Immunoassays facilitate a wide range of rapid, simple and sensitive biochemical detection analyses. We developed a signal-off tuned signal-on (SF-T-SN) colorimetric immunoassay for the detection of the antiviral drug, amantadine (AMD). Using this strategy, the conventional competitive signal-off immunoassay acts as a foundation to induce different immune recognition events in the absence and presence of AMD. The introduction of activity-metal modulated peroxidase-mimicking nanozymes converts the conventional signal-off model to a signal-on model, thereby facilitating drug detection, and overcoming disadvantages with natural enzymes. Importantly, due to switching of the reporting mechanism, and the high catalytic activity of the nanozyme, this “off-to-on” pathway makes the nanozyme-based colorimetric signal proportional to AMD concentrations, thereby circumventing the inherent drawbacks of limited signaling abilities and sensitivities with conventional signal-off immunoassay-based small molecule detection systems. AMD concentrations can be read by the eye or by the optical signals. Based on this protocol, system sensitivity (0.195 ng mL −1 AMD for the naked eye, and 0.134 ng mL −1 AMD for optical detection) not only outperformed reported methods, but it dramatically improved the naked eye observations and UV–vis measurements, when compared with conventional signal-off immunoassays. AMD concentrations spiked into chicken samples were also detected with acceptable recoveries, and relative standard derivations (RSD), indicating this method is highly applicable for biochemical analyses and food safety monitoring.
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危险品化学品经营许可证（带存储）