JOURNAL OF ETHNOPHARMACOLOGY
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2025
·
IF 5.4
Chaoyu Huang,Chen Qian,Zongxian Li,Yuanyuan Qin,Wuning Mo,Faquan Lin
Ethnopharmacological relevance Rosa roxburghii is an ethnic medicinal herb. Folk medicine collections have documented its nourishing and strengthen effects. It has been used to improve reproductive health, but scientific evidence supporting its efficacy and mechanisms remains limited. Aim of this study Endocrine-disrupting chemicals, such as di-(2-ethylhexyl) phthalate (DEHP), are known to impair male reproductive health. This study aims to investigate the protective effects of raw Rosa roxburghii juice (RRJ) on DEHP-induced reproductive toxicity in mice and elucidates its underlying mechanisms. Materials and methods Using a DEHP-induced murine model of reproductive damage, we evaluated the effects of RRJ on sperm quality, testicular histopathology, reproductive endocrine function, oxidative stress, inflammation, apoptosis, and DNA damage. Network pharmacology analysis was performed to identify the active components, targets, and mechanisms underlying the therapeutic effects of Rosa Roxburghii . Results Our data demonstrated that RRJ significantly improved sperm quality, alleviated testicular atrophy, restored endocrine disorders, and mitigated oxidative stress, inflammation, and apoptosis in testicular tissues. Additionally, RRJ reduced testicular and sperm DNA damage, as evidenced by decreased γ-H2AX expression and DNA fragmentation index. Network pharmacology analysis identified quercetin, apigenin, luteolin, kaempferol, eriodictyol, and ellagic acid as the key bioactive compounds in RRJ, with the PI3K/AKT signaling pathway playing a crucial role in its therapeutic effects. Western blotting confirmed that RRJ reversed DEHP-induced suppression of the PI3K/AKT pathway. Conclusions This study demonstrates that RRJ protects against DEHP-induced reproductive toxicity through antioxidant, anti-inflammatory, and anti-apoptotic mechanisms, mediated in part by the PI3K/AKT signaling pathway. This work provides the first comprehensive evidence of the protective effects of Rosa roxburghii against male reproductive system damage and its underlying mechanisms.
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Food Chemistry-X
·
2026
·
IF 9.2
Xiaoli Zhou,Yanli Wang,ShengYan Zhang,Bin Du,Lingshuai Meng
This study investigated the in vivo physiological effects of solid-state fermented (SSF) Rosa roxburghii Tratt. pomace (F-RRTP) on host metabolism and gut microbiota. Rats were fed diets supplemented with 1% ( w/w , total diet basis) unfermented RRTP or F-RRTP. Compared with unfermented RRTP, F-RRTP showed a relative increase in serum HDL-C and improved antioxidant-related parameters, as indicated by higher serum SOD and hepatic T-AOC activities. Gut microbiota analysis showed that F-RRTP altered the microbial community structure, including a reduced Firmicutes / Bacteroidetes ratio and a lower relative abundance of Proteobacteria , a taxon commonly associated with gut dysbiosis. Notably, F-RRTP enriched putative butyrate-producing genera, including Ruminococcus and Allobaculum , as well as Bifidobacterium , and this was accompanied by a higher fecal butyrate level. Serum metabolomics further revealed distinct metabolic alterations in the F-RRTP group, including changes in flavonoid-related metabolites, tryptophan metabolites such as serotonin and indole-3-lactic acid, and several metabolites associated with antioxidant status. It suggested coordinated associations among gut microbiota, serum metabolites, and host physiological indices, implying that fermentation-induced changes in pomace properties may contribute to the observed effects. Collectively, these findings indicate that SSF may enhance the functional potential of RRTP and support its further development as a value-added food or feed ingredient with potential metabolic health benefits.
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RSC Advances
·
2025
·Vol. 15
·期 26
·20872
·
IF 4.6
Fangli Lou,Jinju Wang,Xiang Wang,Mingxian Zhang,Jie Yuan
A key hurdle in optimizing sodium-ion battery (SIB) performance is developing cost-effective, highly stable anode materials. Biomass-derived carbon has emerged as a leading candidate due to its recyclability and structural benefits. As a cellulose-rich biomass waste, Rosa roxburghii pomace (RRP) can be transformed into hard carbon materials with porous structures and suitable interlayer spacing through carbonization and structural regulation, exhibiting excellent sodium storage performance. In this study, we developed high-performance hard carbon materials through a straightforward thermal decomposition method, starting with RRP as the primary feedstock. To fine-tune the structure, melamine served as a nitrogen source, effectively weaving nitrogen heteroatoms into the carbon framework. Boasting a sizable interlayer carbon distance (0.69 nm), RRP-800-N exhibits a remarkable reversible capacity (223.7 mAh g −1 at 31 mA g −1 ). It also showcases a high initial coulombic efficiency, clocking in at 99.11% at 31 mA g −1 , and demonstrates a stable cycling life, retaining 237.1 mAh g −1 after 100 cycles at 31 mA g −1 . The experimental results indicate that the high-activity N groups and disordered amorphous structure of RRP-800-N offer sufficient active sites and enhance conductivity. In addition, the abundant mesopores provide continuous ion transport pathways, shortening the diffusion distance of Na + from the electrolyte to the bulk material. This research shows how RRP biomass waste can be transformed into affordable, sustainable hard carbon, proving its potential as a durable sodium-ion battery anode. A key hurdle in optimizing sodium-ion battery (SIB) performance is developing cost-effective, highly stable anode materials.
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Arabian Journal of Chemistry
·
2021
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IF 5.165
Ruimin Wang,Ruiping He,Zhaohui Li,Xue Lin,Lu Wang
Rosa roxburghii Tratt. leaves (RRLs) have been exploited as a alternative tea product in China owing to its various biological properties. A comparative study was performed for the first time on high-performance liquid chromatography quadrupole orbitrap tandem mass spectrometry (HPLC-Q-Orbitrap-MS/MS) phenolic profiles, antioxidant, α -glucosidase inhibitory ( α -GIA) and anti-bacterial activities of the RRLs extracts extracted by traditional and eco-friendly solvents. The RRLs extracts extracted with four screened deep eutectic solvents (DES) showed higher total phenolic content (TPC, 167.48–190.14 mg GAE/g DW) and moderate total flavonoid content (TFC, 3.78–4.11 mg RE/g DW), while the RRLs extracts extracted with choline chloride-1,2-propanediol, choline chloride-levulinic acid, and 50% methanol/ethanol extracts had the highest TFC. Ethyl acetate extracts had the lowest TPC and TFC. Additionally, the phenolic constituents of the RRLs extracts were identified and quantified via HPLC-Q-Orbitrap-MS/MS and HPLC-DAD methods. A total of 30 phenolic compounds were identified in RRLs extracts. Among them, arbutin, gallic acid, (+)-catechin, 3-hydroxybenzoic acid, quercetin-3- O -galactoside and myricetin were the representative ones. The selected DES (especially for choline chloride-lactic acid and choline chloride-levulinic acid) extracts exhibited higher 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS +• ) and 2,2-diphenyl-1-picrylhydrazyl (DPPH • ) scavenging activities, cupric reducing antioxidant capacity (CUPRAC), ferric reducing antioxidant power (FRAP) except for reducing power, α -GIA and anti-bacterial activity as compared with the extracts extracted with other solvents. Multivariate analysis results revealed that the extraction solvents significantly affected the phenolic constituents and biological activities of the RRLs extracts. The present study presented eco-friendly solvents for high-efficient extraction of the phenolic metabolites from RRLs. RRLs as a potential source enriched in phenolic constituents can be applied in the health, cosmetic and pharmaceutical industries.
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Journal of Food Measurement and Characterization
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2025
·
IF 3.3
Wen Yao,Chen Geping
The polysaccharide was extracted by ultrasonic-assisted method with deep eutectic solvent from Rosa roxburghii Tratt. (RRT). The single-factor experiment was carried out first, and then the response surface experiment was designed to obtain the optimal process conditions. The optimal extraction conditions were the molar ratio of choline chloride to oxalate of 1:1, the water content of deep eutectic solvent (DES) of 27%, the solid-liquid ratio of 1:52 g/mL, time of 99 min, and the power of 240 W, with the extraction yield of 3.397%. The yield of RRT polysaccharide (RRTP) was 2.693% and 2.521% by water immersion and ultrasonic water extraction, respectively, indicating that the DES extraction method (3.397%) is superior to these two methods, and the optimization effect is good. After purification by the DEAE-SephaRosa (DEAE) column and Sephadex G-75 column, the biological activities of the extracted polysaccharides were tested. At a concentration of 6.4 mg/mL, RRTPs-1 inhibited α-glucosidase by 26%. The IC 50 value of RRTPs-1 against α-amylase was 2.94 mg/mL. At 3.2 mg/mL, RRTPs-1 inhibited DPPH· by 22.36%. The IC 50 value of RRTPs-1 on ABTS· + scavenging was 0.055 mg/mL. The analysis of monosaccharide composition shows that it contains glucose, rhamnose, xylose, arabinose, mannose, galactose, galacturonic acid, and glucuronic acid, with the molar ratio of 1.00: 14.14: 4.28: 5.63: 15.47: 64.50: 5.03: 41.43. In this paper, the process of extracting RRTP with deep eutectic solvent was studied and optimized. The activity study showed that RRTP has certain hypoglycemic and antioxidant activities. Therefore, this study can provide an experimental basis for the establishment of an industrialized production process of polysaccharides and the study of their biological activities.
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INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
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2017
·Vol. 52
·期 4
·897-905
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IF 1.64
Fangfang Zeng,Zhiwei Ge,Jarukitt Limwachiranon,Li Li,Simin Feng,Yansheng Wang,Zisheng Luo
Summary The content of bioactive phytochemicals, antioxidant activity and tyrosinase inhibitory activity of the hydroalcoholic extracts of Rosa roxburghii were determined. Yellow fruits of cultivated R. roxburghii showed the highest phenolic content (154.81 mg gallic acid g −1 ), and the green fruits of wild R. roxburghii showed higher content of flavonoid and triterpenoid. Rosa roxburghii fruits from different cultivars and maturity stages all demonstrated as good antioxidant agents and tyrosinase inhibitors, with IC50 value about twice of the positive standard in the DPPH assay and triple of the standard in the tyrosinase inhibitory activity assay. Nineteen compounds, mainly ellagic acids and its derivatives, flavonoids and their glycosides were identified by UPLC-Triple-TOF/MS analysis. As the first study of bioactive phytochemicals of R. roxburghii by UPLC-MS, the present research may provide valuable information for fulfilling the potential of R. roxburghii in the functional food area.
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INTERNATIONAL IMMUNOPHARMACOLOGY
·
2025
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IF 4.7
Xiaomeng Wang,Ting Zhou,Shaolin Huang,Heting Zhou,Yihan Ling,Tao Chen,Shuwen Zhang,Wenxi Wang,Chuan Wu,Wenya Yin
Rosa roxburghii Tratt (RRT), a fruit with dual medicinal and nutritional applications, exhibits therapeutic potential against pulmonary fibrosis, yet the specific bioactive constituents underlying this effect remain uncharacterized. This study employed an integrated spectrum-effect relationship to systematically identify RRT's principal anti-pulmonary fibrosis components. Our findings demonstrate that five different polar extracts of RRT (RRTEs) differentially attenuated bleomycin-induced pulmonary fibrosis in murine models, with the ethyl acetate fraction (EAE) showing superior therapeutic efficacy. HPLC-Q-Exactive Orbitrap MS identified 56 compounds, and screened out four active ingredients related to anti-pulmonary fibrosis by spectrum-effect relationship. In vitro experiments revealed that ellagic acid, gallic acid and syringic acid inhibited fibroblast migration, attenuated intracellular ROS overproduction, and downregulated the expression levels of α-SMA and collagen I. In summary, we established for the first time a spectrum-effect relationship between RRT and pulmonary fibrosis, elucidated the key components, and provided a foundation for future clinical applications.
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LWT-FOOD SCIENCE AND TECHNOLOGY
·
2024
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IF 6
Jiaqi Wang,Binbin Li,Guilan Jiang,Silin Li,Jingyi Zhu,Jia Huang,Yuting Song,Zhuhong Ding
Lactobacillus acidophilus GIM1.208 (LA) β-glucosidase (BGL) converts glycosides from Rosa roxburghii Tratt (RRT) to release more active aglycones. This study purified LA BGL and investigated it interaction with rosamultin in RRT. The isolated and purified BGL in the study had higher purity and activity, and the proportion of ordered structure in its secondary structure was greater than 60%, indicating better structural stability. The hydrolysis of purified BGL hydrolyzed rosamultin to tormentic acid at a rate of 25.2% (p<0.01), and the hypoglycemic activity of tormentic acid was higher than that of rosamultin. Rosamultin causes BGL to undergo massive aggregation and enhances the hydrogen bonding network structure. Thermodynamic analyses indicate that their binding is a non-spontaneous exothermic entropy-decreasing reaction, with the main forces being hydrogen bonding, van der Waals forces, or protonation forces. Additionally, their thermodynamic binding constant Kd=2.976×10 -5 mol/L and dissociation constant K D =2.67×10 -5 mol/L indicate that the two have high affinity and strong interaction. In conclusion, BGL has the ability to hydrolyze rosamultin to release tormentic acid and they have a strong interaction. The results of this study can provide a reference for the study of glycoside changes and their interactions in RRT fermented by LA.
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INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
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2024
·
IF 5
You Luo,Ruling Tang,Han Qiu,Angxin Song
Rosa roxburghii Tratt fruits (RRT) exhibit extremely high nutritional and medicinal properties due to its unique phytochemical composition. Probiotic fermentation is a common method for processing fruits. Variations in the non-volatile metabolites and bioactivities of RRT juice caused by different lactobacilli are not well understood. Therefore, we aimed to profile the non-volatile components and investigate the impacts of L. plantarum fermentation (LP) and L. paracasei fermentation (LC) on RRT juice (the control, CG). There were both similarities and differences in the effects of LP and LC on RRT juice. Both of the two strains significantly increased the content of total phenolic, total flavonoid, and some bioactive compounds such as 2-hydroxyisocaproic acid, hydroxytyrosol and indole-3-lactic acid in RRT juice. Interestingly, compared with L. paracasei , L. plantarum showed better ability to increase the content of total phenolic and these valuable compounds, as well as certain bioactivities. The antioxidant capacity and α-glucosidase inhibitory activity of RRT juice were notably enhanced after the fermentations, whereas its cholesterol esterase inhibitory activity was reduced significantly. Moreover, a total of 1466 metabolites were identified in the unfermented and fermented RRT juices. There were 278, 251 and 134 differential metabolites in LP vs CG, LC vs CG, LC vs LP, respectively, most of which were upregulated. The key differential metabolites were classified into amino acids and their derivatives, organic acids, nucleotides and their analogues, phenolic acids and alkaloids, which can serve as potential markers for authentication and discrimination between the unfermented and lactobacilli fermented RRT juice samples. The KEGG enrichment analysis uncovered that metabolic pathways, purine metabolism, nucleotide metabolism and ABC transporters contributed mainly to the formation of unique composition of fermented RRT juice. These results provide good coverage of the metabolome of RRT juice in both unfermented and fermented forms and also provide a reference for future research on the processing of RRT or other fruits.
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JOURNAL OF FOOD BIOCHEMISTRY
·
2024
·Vol. 2024
·期 1
·3900785
·
IF 3.5
Wei Ren,Jian Xu,Yongping Zhang,Yanyan Miao,Yao Liu
The aim of this study is to investigate efficient, cost-effective, and high-quality drying technologies for Rosa roxburghii Tratt. Five drying methods—hot air drying (HAD), microwave drying (MD), vacuum drying (VD), microwave vacuum drying (MVD), and freeze vacuum drying (FVD), were employed to dry Rosa roxburghii Tratt. The study focused on evaluating drying characteristics, colors, texture and structure, and microstructure, as well as the content of active ingredients and antioxidant activity of Rosa roxburghii Tratt. The results of drying kinetics indicated that the Wang and Singh model was the most suitable for FVD, while the Page model was optimal for the other four drying methods. Rosa roxburghii Tratt subjected to HAD exhibited the highest energy consumption per unit but the lowest ΔE and the highest TFC. The microstructure of Rosa roxburghii Tratt under different drying methods was analyzed. Rosa roxburghii Tratt dried using MD experienced serious cell rupture, resulting in a large loss of active ingredients and the worst antioxidant ability, but it had the fastest drying rate. On the other hand, Rosa roxburghii Tratt dried under VD had the microstructure with the least pore space, leading to slower drying speed and the highest total polyphenol content. Rosa roxburghii Tratt dried using MVD had the least hardness and chewiness, with slightly better Δ E and active ingredient content compared to MD. FVD Rosa roxburghii Tratt took the longest time to dry, with the greatest Δ E difference, hardness, and chewiness, but it had the highest Vitamin C content and the most powerful antioxidant ability. Principal component analysis (PCA)–entropy weighting method was used to provide comprehensive scores for the different drying methods, with FVD, HAD, VD, MVD, and MD were 0.8107, 0.7037, 0.4499, 0.2746, and 0.0586 respectively. Through comprehensive analysis, we demonstrated that the quality of Rosa roxburghii Tratt obtained via FVD and HAD is satisfactory. Additionally, HAD is characterized by its economic efficiency and convenience, providing valuable insights for the industrial production of Rosa roxburghii Tratt drying.
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Nutrients
·
2025
·Vol. 17
·期 21
·3463
·
IF 5
Yang Liu,Jingzhong Zheng,Xin Zheng,Dan Zhou,Hang Ma,Xue Zhou,Fahuan Ge
Background:Cili (Rosa roxburghiiTratt) is a unique fruit native to China’s Yunnan–Guizhou Plateau, rich in vitamin C, polyphenols, and triterpene, with broad health-promoting effects. Although cili’s hepatoprotective properties are reported, the bioactive components and underlying mechanisms remain poorly defined.Methods:We enriched proanthocyanidins from cili using column chromatography, identified their components via UPLC-Q-TOF-MS/MS, and validated their anti-liver fibrosis effects through in vitro and in vivo experiments.Results:Herein, we developed a novel proanthocyanidin-rich cili fruit extract (PACs-CFE) containing 84.2% total proanthocyanidins, comprising catechins, epicatechins, and diverse B-type dimers, trimers, tetramers, and gallate esters, as characterized by UPLC-Q-TOF-MS/MS. PACs-CFE inhibited LX-2 activation, suppressed collagen III and α-SMA expression, and induced ferroptosis via mitochondrial injury, reactive oxygen species accumulation, and GPX4/ferritin downregulation. In vivo, PACs-CFE ameliorated liver fibrosis, restored hepatic architecture, and improved serum alanine aminotransferase, aspartate aminotransferase, and bilirubin profiles. Moreover, PACs-CFE modulated the TGF-β1/Smad3 signaling pathway and beneficially reshaped the gut microbiota, enriching anti-inflammatory and hepatoprotective genera while reducing pathogenic taxa.Conclusions:Our findings show that PACs-CFE exerts multi-targeted anti-fibrotic effects through hepatic stellate cell inactivation, ferroptosis induction, TGF-β1/Smad3 suppression, and gut–liver axis modulation. This study provides useful insight into the hepatoprotective potential of cili fruit and supports its development as standardized functional ingredients for liver health.
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FOOD RESEARCH INTERNATIONAL
·
2025
·
IF 8
Mingxiu Long,Hui Wang,Fang Zhang,Yaoqi Ran,Yongfu Li,Jingwen Luo,Zhaojun Chen,Zhuxi Tian,Shuming Tan,Xiong Liu
Rosa roxburghii Tratt fruit polyphenols (RP) possess antioxidant, anti-inflammatory, and immunomodulatory properties, showing potential for ulcerative colitis (UC) treatment. However, their efficacy is limited by bioavailability and targeting efficiency. To address this, RP were encapsulated into acylated chitosan-crosslinked carboxymethyl konjac glucomannan (ACs-CKGM) nanogel (RPNG). Experimental results demonstrated that RPNG significantly enhanced intestinal barrier function by upregulating tight junction proteins (ZO-1, Occludin and Claudin-3) and mucus secretion (MUC2). It effectively mitigated oxidative stress by restoring antioxidant enzymes (SOD, GSH-Px and CAT) while reducing MPO, COX-2 and iNOS activation. Through integrated ELISA, western blot, and transcriptomic analyses, we emonstrated that RPNG specifically inhibited PI3K/Akt/mTOR and NF-κB signaling pathways, decreasing inflammatory cytokines (e.g., TNF-α, IL-6) while increasing anti-inflammatory mediators (e.g., IL-10). Gut microbiome analysis showed RPNG reversed DSS-induced dysbiosis by enriching beneficial bacteria (e.g., Muribaculum sp.) and suppressing pathogens (e.g., Escherichia coli ). These findings highlight RPNG as a novel targeted strategy for UC management, supported by multi-omics mechanistic insights.
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