LANGMUIR
·
2025
·
IF 3.9
Maosheng Sang,Yang Han,Heng Zhang,Zhongyu Li,Quanlong Zhou,Xiaoya Meng,Yuhao Zhang,Yemin Guo,Xia Sun,Surayo Rakhmatzhanovna Usmanova,Jamshed Bobokalonov,Wenping Zhao
An “on–off” electrochemiluminescence (ECL) aptasensor was proposed for the highly sensitive and selective detection of ochratoxin A (OTA) in cereals. The substrate nanomaterial employed in the ECL experiments was titanium carbide (Ti3C2Tx) MXene, while cadmium sulfide quantum dots (CdS QDs) were used as coreactants of the ECL reagent tris (2,2′-bipyridyl) ruthenium(II) (Ru(bpy)32+). Compared to tripropylamine, a more toxic conventional coreactant, CdS QDs can react efficiently with Ru(bpy)32+ in the ECL reaction, and the reaction principles of both were also investigated. The effective connection between CdS QDs and Ru(bpy)32+ via the aptamer resulted in a shortened electron transfer distance, which in turn led to an improvement in ECL intensity and the achievement of the first “on” state. The introduction of OTA resulted in the loss of the effective connection between the CdS QDs and Ru(bpy)32+, which caused the sensor to enter an “off” state. The high ECL intensity of the sensor in the “on” state, in contrast with the low ECL intensity of the “off” state, resulted in an increase in the sensitivity and stability of the sensor. The established aptasensor exhibited a low limit of detection (LOD) of 0.03 ng/mL within a linear range of 0.1 ∼ 100 ng/mL, with spiked recoveries ranging from 93.7% to 105.1%, showing good selectivity, stability, and reproducibility for the detection of OTA in cereals.
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DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2026
·
IF 5.2
Muhammad Saqib,Zhang-Jian Cheng,Si-Yang Lin,Mohib Ullah,Ai-Lin Liu,Yun Lei
Analyte-induced self-enhanced electrochemiluminescence (ECL) is a powerful strategy for simplifying ECL sensing and improving the overall sensitivity without the addition of external coreactants. However, most Ru(bpy) 3 2+ -based ECL systems still rely on external coreactants such as tri- n -propylamine (TPrA) or oxalate, which introduce additional reagents and increase assay complexity. Herein, we report that the moxifloxacin (MFX) can intrinsically function as an efficient coreactant for Ru(bpy) 3 2+ , enabling an external-coreactant-free and self-enhanced ECL system for its sensitive detection. Electrochemical and ECL investigations reveal that MFX participates directly in the ECL pathway through anodic oxidation, generating reactive intermediates that efficiently trigger the excited Ru(bpy) 3 2+ * state. As a result, the Ru(bpy) 3 2+ –MFX system generates ∼17-fold higher ECL intensity than the classical oxalate system and a comparable ECL response to TPrA (∼66%) under identical conditions. Leveraging this intrinsic self-amplification, an ECL method was developed for sensitive and selective detection of MFX. The developed method exhibits two linear dynamic ranges (0.05–200 μM and 200–800 μM) with a limit of detection (LOD, S/N = 3) of 36 nM. The proposed method offers excellent sensitivity, reproducibility, and selectivity against common interferents and ophthalmic excipients. Moreover, validation using commercial ophthalmic formulations demonstrated excellent agreement with HPLC results, supporting the accuracy of the developed method for pharmaceutical quality control. By integrating analyte recognition and signal generation into a single molecular entity, this work opens a new pathway toward simple and green self-enhanced ECL sensing platforms.
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DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Yingjie Zhao,Li Tian,Xin Zhang,Zhuo Sun,Xiangyu Shan,Qian Wu,Ruizhan Chen,Juan Lu
A specificity recognition molecularly imprinted electrochemiluminescence sensor (MIECLS) for determination of clenbuterol (CLB) was established by molecular imprinting technology. In this sensing platform, Fe 2 O 3 was used as the carrier to fix Ru(bpy) 3 2+ , and it could also improve electrochemiluminescence (ECL) signal due to large specific surface area and better electronic transmission efficiency. The introduction of molecular imprinted membranes (MIPs) with CLB as template molecules realized the specificity identification of sensors. The intensity of the ECL signal decreased gradually when the concentration of CLB increased. Based on this characteristic, a novel method for measuring CLB was established, and the related mechanism was studied. Under the optimal conditions, the ECL response was proportional to the logarithm of CLB concentration in the range of 1 × 10 −9 mol L −1 to 1 × 10 −4 mol L −1 with a detection limit of 3.3 × 10 −10 mol L −1 . The results prove that MIECLS has great potential in pork and liver samples testing.
研究领域:
分析化学
·
生物传感
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DOI
高影响因子
Journal of Nanomaterials
·
2023
·
IF 3.791
Xiyuan Tong,Fang Fang,Zhuozhe Li,Kun Qian,Yao Xu,Yifeng E
Acetaminophen (AP) is a commonly used drug that has been detected in groundwater systems in many countries, and has received much attention from researchers in recent years due to its potential environmental impact. In this research, uniformly distributed boron nitride quantum dots (BNQDs) were prepared by a simple ultrasound-solvothermal method. Electrochemical luminescence (ECL) spectroscopy confirmed that BNQDs can act as an effective coreactant to create excellent efficiency in amplifying the ECL intensity of ruthenium-based ECL system. Based on the excited state of and the energy transfer quenching of AP oxidation products in the luminescent system, an AP concentration-quenched drug sensor was successfully constructed. For this sensor, a wide linear dynamic range and low detection limits (5.0 × 10−7−1.0 × 10−5 mol/L and 4.8 × 10−9 mol/L, respectively) were achieved. This ECL drug sensor has excellent performance in the accurate determination of AP content, relieving the stress of the previous AP detection process, and has good reproducibility and recovery in actual sample measurements.
研究领域:
分析化学
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环境科学
·
药学
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DOI
高影响因子
Journal of Materials Chemistry C
·
2023
·Vol. 11
·期 38
·13106-13112
·
IF 6.4
Mingquan Guo,Jiangnan Shu,Dexin Du,Yisha Wang,Hua Cui
Potential resolved electrochemiluminescence (ECL) nanoluminophores are very important for precise analysis and simultaneous determination of multiple targets. Designing ideal potential resolved ECL nanoluminophores, generating multiple high efficiency ECL emissions without overlap and interference of potential and wavelength, is a challenging subject. Herein, we developed a new nanoluminophore, meso-tetra (4-carboxyphenyl) porphine (TCPP)-functionalized chitosan/Ru(bpy)32+/SiO2 (CRuSiO2) (TCPP@CRuSiO2), with cathode and anode ECL emissions by modifying TCPP on the surface of CRuSiO2 nanoluminophores. The TCPP@CRuSiO2 nanoluminophores presented a uniform sphere morphology (∼50 nm). They exhibited two ECL emissions of 695 nm and 610 nm at −1.20 V and 1.15 V, respectively, by using K2S2O8 and tripropylamine (TPrA) as coreactants. Subsequently, the relevant ECL mechanisms are proposed. In the negative potential range, sulfate radicals (SO4˙−) reacted with TCPP−, generated by the electro-reduction of S2O42− and TCPP, to form the excited state TCPP*, resulting in ECL-1. In the positive potential range, Ru(bpy)33+ and TPrA˙, obtained by electro-oxidation of Ru(bpy)32+ and TPrA, reacted with each other to produce the excited state Ru(bpy)32+*, leading to ECL-2. The synthesized TCPP@CRuSiO2 possesses not only potential and wavelength resolved ECL emissions, but also the advantages of easy synthesis, high ECL efficiency, good stability, and easy film formation on the electrode surface. This work enriches ECL nanoluminophores, which are of important application potential in precise analysis and multiplex analysis.
研究领域:
分析化学
·
材料科学
·
电化学
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DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2023
·
IF 4.8
Kaida Kuang,Ya Li,Yu Ji,Yang Liu,Nengqin Jia
In this work, a quenched molecularly imprinting-electrochemiluminescence (MIP-ECL) sensor based on Ru(bpy) 3 2+ @ZnO-Au nanocomposites was constructed to realize the sensitive detection of acrylamide (AM). Ru(bpy) 3 2+ @ZnO-Au nanomaterials show high ECL efficiency. AuNPs has excellent properties and can improve the properties of electrochemiluminescence by promoting the electron transfer process of the luminescent body. The MIP polymer film was formed on the surface of the material and eluted with eluent, leaving an AM imprinting cavity as a specific recognition site. When adding different concentrations of AM, it was specifically recognized by the MIP membrane, and the ECL signal decreased gradually, thus realizing the sensitive detection of AM. Under the optimal conditions, the detection ranges of the MIP-ECL sensor are 1–10 8 nM and the limit of detection (LOD) is 0.123 nM. Therefore, this sensor proposed in this work opens up a new way for the sensitive detection of AM in food.
研究领域:
分析化学
·
食品安全检测
·
纳米材料科学
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DOI
高影响因子
ELECTROCHIMICA ACTA
·
2024
·
IF 5.5
Yuhao Zhang,Luting Yan,Lei Tian,Qi Guo,Maosheng Sang,Shouyi Dou,Falan Li,Qingqing Yang,Xia Sun,Yemin Guo,Yanyan Zhang
Vibrio parahaemolyticus ( VP ) is one of the most common pathogens in aquatic products, and its study using electrochemiluminescence is still relatively rare. In this study, an electrochemiluminescent (ECL) biosensor was developed to detect VP in aquatic products using AuNPs modified PEI/MWCNTs (AuNPs@PEI/MWCNTs) as a substrate material and ordered mesoporous carbon (OMC) adsorbed Ru(bpy) 3 2+ (OMC@Ru(bpy) 3 2+ ) as a luminescent material. To increase the conductivity of the biosensor and offer a favorable loading environment for the immobilization of the luminescent probe, AuNPs@PEI/MWCNTs are employed as the substrate. Meanwhile, the OMC@Ru(bpy) 3 2+ functionalized DNA probe acts as a luminescent and capture probe to generate ECL signals and specifically recognize different concentrations of VP . The biosensor has good selectivity and stability and has been successfully applied to the detection of VP in scallops. This method not only expands the potential uses of bioprobe-based ECL biosensors, but also offers a new and effective approach used to detect food-borne pathogens in aquatic products.
研究领域:
食品科学
·
生物技术
·
分析化学
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DOI
高影响因子
TALANTA
·
2025
·
IF 6.1
Jin Wang,Xinyu Lu,Huafeng Wang,Yuan Zhong,Zhihui Dai,Tianxiang Wei
In this work, Ru(bpy) 3 2+ -loaded gold nanocage (AuNCs) (Ru–AuNCs) was prepared and found to display a distinct property of electrochemiluminescence (ECL) enhancement under mercury ions (Hg 2+ ) interaction. Based on this, we designed a screen-printed bipolar electrode-ECL (SPBPE-ECL) sensing platform by coupling with the thymine-Hg 2+ -thymine (T-Hg 2+ -T) binding pattern for one-step highly sensitive detection of Hg 2+ . This ECL sensor showed a wide linear detection range (0.75 – 850 μg L −1 ) and low detection limit (0.1290 μg L −1 ) toward Hg 2+ , with a one-step detection procedure and disposable feature, displaying potential applicability in the point-of-care-testing (POCT) of Hg 2+ in the environment. In addition, the Hg 2+ -mediated ECL signal enhancement mechanism of Ru–AuNCs was also investigated. It was confirmed that Hg 2+ interaction etched the cage structure of Ru–AuNCs, which sped up the release of more Ru(bpy) 3 2+ around the sensing electrode. Furthermore, Au–Hg alloy structure was formed on the surface of Ru–AuNCs, which also improved the ECL signal. This target-induced in-situ sensing material surface reconstruction strategy would provide a better design concept for the construction of ECL POCT sensor.
研究领域:
环境科学
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分析化学
·
生物传感
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2020
·
IF 7.1
Dandan Fang,Huizhu Ren,Yitian Huang,Hong Dai,Defu Huang,Yanyu Lin
Herein, an ultrasensitive electrochemiluminescence (ECL) immunosensor based on a high performance probe of Nb 2 CTx MXene immobilized ZnO quantum dots (QDs) was constructed for Thyroglobulin (Tg) detection. ZnO QDs with good biocompatibility and catalytic property was for the first time utilized as coreaction accelerator in Ru(bpy) 3 2+ /S 2 O 8 2− system and enhanced ECL signal. Specifically, ZnO QDs can facilitate S 2 O 8 2− reduction to generate more SO 4 − , and then reacted with cathodic radical of Ru(bpy) 3 2+ , realizing 2-fold enhancement in ECL intensity. Additionally, Nb 2 CTx MXene possesses excellent properties and was used as prominent supporter, improving the loading capacity of Ru(bpy) 3 2+ and ZnO QDs, and accelerating the electron transfer, resulting in ECL signal enhancement. Furthermore, Nb 2 CTx MXene acted as temperature controller and increased the electrode surface temperature by converting laser energy into heat, further amplified ECL signal. Because of the high sensitivity, good specificity and favorable stability, the proposed immunosensor realized sensitive detection of Thyroglobulin and had a great application prospect in bioanalysis and clinical diagnosis.
研究领域:
分析化学
·
生物医学工程
·
临床诊断
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高影响因子
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
·
2015
·Vol. 17
·期 16
·10726-10736
·
IF 4.493
Huan Lin,Dan Liu,Jinlin Long,Zizhong Zhang,Huaqiang Zhuang,Yi Zheng,Xuxu Wang
The role of polymers in artificial photosystems has been studied in detail. The photosystems were composed of tris(2,2′-bipyridyl) ruthenium(II) chloride as a photosensitizer (PS), colloidal Pt stabilized by polymer as a hydrogen-evolving catalyst and sodium ascorbate as an electron donor, without the addition of a traditional molecular electron mediator. Comprehensive insights into the production of hydrogen on irradiation with visible light were achieved. Several polymers, including neutral polyvinyl pyrrolidone, anionic poly(sodium 4-styrene sulfonate) and poly(acrylic acid) not only stabilized the nanoparticles, but were also effective in the production of hydrogen. Under the optimum conditions, an outstanding apparent quantum efficiency of 12.8% for the evolution of hydrogen was achieved. The formation of self-assembled and spatially separated donor–acceptor complexes via the non-covalent intermolecular interaction between PS and the polymer–Pt was pivotal in the efficient conversion of solar energy to hydrogen fuel. Important details of the photo-induced electron and energy transfer processes in the self-assembled artificial photosystems were determined by nanosecond transient absorption spectrometry and time-resolved fluorescence spectrometry. The initial step in the photo-catalytic production of hydrogen was a reductive quenching of the triplet excited state of the PS by sodium ascorbate, leading to a reduced form of PS, which could then be quickly quenched by the polymer. The rate-determining step was the electron transfer from PS to the catalyst via the polymer bridge.
研究领域:
化学
·
能源科学
·
材料科学
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DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2017
·
IF 7.78
Hong Ke,Haifeng Sha,Yinfang Wang,Weiwei Guo,Xin Zhang,Zhiming Wang,Chusen Huang,Nengqin Jia
Electrochemiluminescent (ECL) assay has gradually drawn increasing interest in the biomedical analysis. This paper proposed a new methodology for ultrasensitive and facile detection of Alzheimer's disease marker β-amyloid (Aβ) by fabricating a sandwich-type ECL sensing platform. Herein, electrochemiluminescence resonance energy transfer (ECL-RET) was employed to determine Aβ concentration, which can be attributed to the quenching effect from RET between Ru(bpy) 3 2+ and gold nanorods (GNRs) acting as ECL-RET electron donor and acceptor, respectively. In this protocol, mesoporous carbon nanospheres were adopted to immobilize ECL reactant Ru(bpy) 3 2+ and antibody via nafion to acquire the RET donor nanocomposites (MOCs/nafion/Ru(bpy) 3 2+ /antibody), which were tightly interconnected with epoxy group functionalized Fe 3 O 4 nanoparticles . It is of vital importance that GNRs with exquisite rod shape were synthesized and exhibited a typical absorption peak at 650 nm to quench ECL signal of Ru(bpy) 3 2+ effectively. In addition, the ECL emission decreased linearly with the logarithm of Aβ concentration in a wide linear range from 1.0 × 10 −5 to 100 ng/mL with a detection limit of 4.2 × 10 −6 ng/mL. Furthermore, distinctive and desirable properties were verified to declare the promise for being applicable to analyze the Aβ content in real Alzheimer's cerebrospinal fluid samples with satisfactory results.
研究领域:
生物医学工程
·
分析化学
·
纳米技术
·
诊断学
·
神经退行性疾病
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2019
·
IF 6.393
Li Tian,Xuerong Wang,Kexin Wu,Yue Hu,Yue Wang,Juan Lu
A ultrasensitive electrochemiluminescence (ECL) biosensor was designed for the detection of dopamine using ZnSe quantum dots and Ru(bpy) 3 2+ as the signal probes, and graphite oxide and multi-walled carbon nanotube as the supporting matrix. The biosensor exhibits efficient and stable anodic ECL signal (at 1.10 V vs. Ag/AgCl) in phosphate buffer solution (pH 9.0) containing Tripropyl amine. The ECL emission decreases with adding dopamine, which can be attributed to the quenching effect from interaction between ZnSe and dopamine. The possible reaction mechanism of the ECL system is studied, and a method for the determination of dopamine is proposed. Under the optimal experimental conditions, the ECL emission decreases linearly with the logarithm of dopamine concentration in a wide linear range from1 × 10 −9 to 1 × 10 -5 mol L −1 with a detection limit of 6 × 10 -10 mol L -1 (S/N = 3). The biosensor is also successfully applied to detect dopamine in human blood serum samples.
研究领域:
生物传感
·
分析化学
·
材料科学
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高影响因子
危险品化学品经营许可证（带存储）