MOLECULAR BIOLOGY REPORTS
·
2026
·Vol. 53
·期 1
·1301
·
IF 3.2
Zhang Chunmei,Wang Shuai,Zhang Xiaoyun,Liu Yan,Chen Yixiang
Background Acrylamide (ACR) is an important chemical raw material, and its toxic effects have been confirmed in vitro and in vivo. However, only a few studies have investigated ACR-induced liver injury. As a naturally occurring flavonoid widely distributed across the plant kingdom, rutin (Rut) possesses notable pharmacological properties. This study aimed to demonstrate its therapeutic potential in mitigating ACR-induced hepatic injury in rats. Accordingly, an intervention model was established to explore the mechanistic basis of the protective effects of Rut against ACR-induced liver injury. Methods The experimental design comprised five cohorts ( n = 10), consisting of 50 male Sprague–Dawley rats randomly assigned to each group: (1) Control (0.5% CMC-Na + ddH 2 O), (2) ACR (20 mg/kg/day via gavage), (3) Rut-L (100 mg/kg) + ACR, (4) Rut-M (200 mg/kg) + ACR, and (5) Rut-H (400 mg/kg) + ACR. Interventions lasted for 21 days. The body weights of the animals were monitored daily. The liver coefficient (liver weight/body weight) was calculated after euthanasia. Commercial assay kits were used to determine serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities and hepatic Superoxide Dismutase (SOD), Glutathione (GSH), and Malondialdehyde (MDA) levels. Histopathological changes were assessed using Hematoxylin and eosin and Masson staining. Tumor Necrosis Factor-α (TNF-α) and cleaved caspase-3 expression levels were analyzed using immunohistochemistry. Results Compared with the Control group, ACR exposure attenuated the rate of body weight accrual ( P < 0.05) and the liver-to-body weight ratio ( P < 0.05), elevated serum ALT (↑34.8%, P < 0.05) and AST (↑47.7%, P < 0.05), decreased hepatic SOD (↓48.9%, P < 0.05) and GSH (↓17.7%, P < 0.05), and increased MDA (↑38.8%, P < 0.05). High-dose Rut reversed these effects by increasing body weight gain ( P < 0.01), liver coefficient (↑29.4%, P < 0.05), SOD (↑32.1%, P < 0.05), and GSH (↑12.1%, P < 0.05), while reducing ALT (↓22.9%, P < 0.01), AST (↓16.8%, P < 0.01), and MDA (↓9.0%, P < 0.05) levels. Histopathological analysis demonstrated reduced hepatocyte necrosis and collagen deposition in the Rut-treated group. Immunohistochemistry revealed that ACR increased TNF-α (↑121.4%, P < 0.001) and cleaved caspase-3 (↑97.8%, P < 0.001) expression, which was suppressed by Rut (TNF-α: ↓48.2%, P < 0.01; cleaved caspase-3: ↓39.5%, P < 0.01). The effect sizes (Cohen’s d) ranged from 1.2 to 3.6, indicating robust effects. Conclusion Rut exhibits a marked protective effect against ACR-induced hepatic injury. Therefore, Rut may be considered a potential agent for preventing ACR-induced liver injury in rats.
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Frontiers in Bioengineering and Biotechnology
·
2020
·
IF 3.644
Wu Haitao,Su Manlin,Jin Hui,Li Xinyu,Wang Puyu,Chen Jingxiao,Chen Jinghua
In this paper, we fabricated rutin-loaded silver nanoparticles (Rutin@AgNPs) as the nano-anticoagulant with antithrombotic function. The serum stability, anticoagulation activity, and bleeding risk of Rutin@AgNPs were evaluated. The results showed Rutin@AgNPs had good serum stability, hemocompatibility, and cytocompatibility. The anticoagulation activity of rutin was maintained, and its stability and aqueous solubility were improved. The Rutin@AgNPs could provide a sustained release to prolong the half-life of rutin. The results of the coagulation parameter assay and thrombus formation test in mice model showed that the activated partial thromboplastin time and prothrombin time were prolonged, and Rutin@AgNPs inhibited the thrombosis in the 48 h period. Moreover, the limited bleeding time indicated that the Rutin@AgNPs significantly minimized the hemorrhage risk of rutin. This Rutin@AgNPs is a potential anticoagulant for antithrombotic therapy.
研究领域:
纳米医学
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药理学
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生物材料科学
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高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Shuai Dong,Shu-Min Hu,Si-Jia Yu,Shaobo Zhou,Tao Zhou
Rutin is a flavonoid polyphenol with excellent biological activity, but due to its instability and poor water solubility, the utilization rate is reduced in vivo . Preparation of rutin microcapsules from soybean protein isolate (SPI) and chitosan hydrochloride (CHC) by composite coacervation can improve this restriction. The optimal preparation conditions were as follows: the volume ratio of CHC/SPI 1:8, pH 6, and total concentration of CHC and SPI 2 %. The rutin encapsulation rate and loading capacity of the microcapsules were 90.34 % and 0.51 % under optimal conditions. The SPI-CHC-rutin (SCR) microcapsules had a gel mesh structure and good thermal stability , and the system was stable and homogeneous after 12 d storage. During in vitro digestion, the release rates of SCR microcapsules in simulated gastric and intestinal fluids were 16.97 % and 76.53 %, respectively, achieving a targeted release of rutin in intestinal fluids; and the digested products were found to exhibit superior antioxidant activity to that of free rutin digests, indicating a good protection of microencapsulation on the bioactivity of rutin. Overall, SCR microcapsules developed in this study effectively enhanced the bioavailability of rutin. The present work provides a promising delivery system for natural compounds with low bioavailability and stability.
研究领域:
食品科学
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生物化学
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PubMed
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CRYSTAL GROWTH & DESIGN
·
2024
·
IF 3.2
Bingrui Zhang,Lei Tang,Fanyu Tian,Qiaoce Ding,Ziyi Hu,Jian-Rong Wang,Xuefeng Mei
Rutin is a natural compound that is widely distributed in various plants. Increasing lines of evidence have proved that rutin has a beneficial effect on cardiovascular health, oxidative stress, and blood glucose control. However, the application of rutin is limited due to its poor solubility and low oral bioavailability. To improve the bioavailability of rutin, two cocrystals of rutin with l-proline and d-proline were prepared successfully, and multiple characterization methods were utilized to study the physicochemical properties of rutin and its cocrystals. The powder dissolution in vitro and the pharmacokinetic behavior in vivo were also evaluated. The results indicated that Rut-l-Pro exhibited a significantly improved solubility, and the oral bioavailability also had great improvement; the AUC0–10h of Rut-l-Pro was 5.6-fold that of rutin, and Cmax was 3.8 times. As a result of the improvement of rutin bioavailability, Rut-l-Pro performed better blood glucose control and cardioprotective activities.
研究领域:
药剂学
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生物化学
·
药物代谢动力学
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高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2022
·
IF 6.056
Alkassoumi Hassane Hamadou,Jiyao Zhang,Chen Chao,Bin Xu
This work evaluated the effect of pectin (P) and chitosan (CH) dual coating on rutin (R) loaded nanoliposomes (NLs). Besides R loaded NLs (RNLs), CH coated RNLs (CH-RNLs) and P coated CH-RNLs (P–CH-RNLs) were prepared. The results showed a size increase of NLs due to the polymer coatings, confirming a successful deposition of CH and P onto the surfaces of NLs. A surface charge reversal was observed after coating RNLs and CH-RNLs with CH and P, respectively. The coating resulted in a significant decrease in encapsulation efficiency (EE) from 88.19% in RNLs to 78.89% in CH-RNLs and 73.44% in P–CH-RNLs. Due to protection provided by the P–CH barrier, P–CH-RNLs displayed better thermal, pH, ionic and oxidative stability. NLs were more stable at 4 °C as compared to 25 °C over 8 weeks, and P–CH-RNLs exhibited the best performance overall. This study revealed P–CH coating as a potential route to produce stable NLs for the delivery of hydrophobic rutin.
研究领域:
药剂学
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食品科学
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材料科学
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高影响因子
JOURNAL OF FOOD BIOCHEMISTRY
·
2019
·Vol. 44
·期 1
·e13086
·
IF 1.358
Hui Liu,Yang Jiang,Hui Guan,Feng Li,Dongxiao Sun-Waterhouse,Yilun Chen,Dapeng Li
Rutin is a bioflavonoid found in many plants and derived foods, accordingly, rutin likely interacts with α-amino acids such as Lys, Ile, His or Glu to give Maillard reaction products (MRPs). The heated rutin-Lys system exhibited highest brown intensity and in vitro antioxidant activities. The 30–50 kDa rutin-Lys fraction had higher in vitro antioxidant activities than the other fractions, and at a dose of 0.4 mg/ml preserved over 90% cell viability for HepG2 cells exposed to H 2 O 2 . The dose-dependent protective effects against H 2 O 2 -induced oxidative stress of the rutin-Lys MRPs may involve the inhibition of reactive oxygen species generation, enhancement of the superoxide dismutase and catalase activities, along with the activation of the Nrf2-dependent pathway and upregulation of phase II antioxidant genes (including NQO1, HO-1, GCLG, and GCLM). Practical applications Rutin is widely distributed in vegetables and grains. The Maillard reaction is a common reaction occurring during food processing, and produces Maillard reaction products (MRPs) with distinct processing and biological properties. This study shows that a 30-min thermal treatment at 120°C generates antioxidative MRPs in the rutin-Lys, rutin-His, rutin-Ile and rutin-Glu model systems, which can directly inhibit reactive oxygen species generation and enhance SOD and CAT activities while activating the Nrf2-dependent pathway and upregulating the expression of phase II detoxifying antioxidant genes. Therefore, for food systems containing phenolic antioxidants and proteins (such as rutin and Lys), one may enhance the antioxidant properties of these food systems through a 30-min thermal treatment at 120°C. Also, the resultant rutin-Lys MRPs may be isolated and used as commercial preparations of natural antioxidants.
研究领域:
食品科学
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营养学
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生物化学
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药理学
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PubMed
高影响因子
Recent Patents on Nanotechnology
·
2020
·Vol. 14
·期 1
·35-41
·
IF 0.977
Li Na,Qian Yongfang,Zhang Zhen,Wang Ying,Lve Lihua,Wei Chunyan
Background: The electrospinning and the bubble electrospinning provide facile ways for the fabrication of functional nanofibers by incorporating rutin/hydroxypropyl-β-cyclodextrin inclusion complex (RT/HP-β-CD-IC) in Polyvinyl Alcohol (PVA). Few patents on incorporation of
rutin and cyclodextrin in nanofibers has been reported. Objective: The study aimed at increasing the loading amount of rutin in the electrospun nanofibers to obtain ultraviolet resistant property. Methods: Rutin was encapsulated in the cavity of RT/HP-β-CD and formed an inclusion
complex. Fourier Transform Infrared Spectroscopy (FTIR) and Differential Scanning Calorimeter (DSC) was used to verify the formation of inclusion complexes. Results: The results showed that the inclusion between rutin and HP-β-CD had been successfully formed. The surface morphologies
of nanofibrous membranes were characterized by Scanning Electron Microscope (SEM), which indicated that adding RT/HP-β-CD inclusion complexes had little influence on the morphologies and diameters of the fibers. Ultraviolet resistant results also confirmed the inclusion complex had increased
the loading amount in the final nanofibrous mats, and thus had good ultraviolet resistant properties. Conclusion: The formed inclusion complexes had obviously enhanced the loading amount of rutin in electrospun PVA nanofibers, indicating that encapsulation of rutin in the cavity of HP-β-CD
is a good way to increase the loading amount.
研究领域:
材料科学
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药学
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PubMed
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2024
·Vol. 25
·期 21
·11404
·
IF 4.9
Changxu Wu,Jinwu Zhang,Shisen Yang,Chunzi Peng,Maojie Lv,Jing Liang,Xiaoning Li,Liji Xie,Yingyi Wei,Hailan Chen,Jiakang He,Tingjun Hu,Zhixun Xie,Meiling Yu
Rutin is a flavonoid compound with potential for treating Alzheimer’s disease, preventing brain damage, mitigating cerebral ischemia–reperfusion injury, and exhibiting anti-glioblastoma activity. However, its efficacy is limited by its low solubility, poor bioavailability, and limited permeability across the blood–brain barrier (BBB). To enhance the bioavailability and brain-targeting ability of Rutin, transferrin-modified Rutin liposome (Tf-Rutin-Lip) was developed using liposomes as a delivery system. Rutin liposomes were prepared using the thin-film dispersion method, and the preparation conditions were optimized using the response surface methodology. Then, transferrin (Tf) was incorporated into the liposomes through covalent modification, yielding Tf-Rutin liposomes. The toxicity of these liposomes on bEnd.3 cells, as well as their impact on the tight junctions of these cells, was rigorously evaluated. Additionally, in vitro and in vivo experiments were conducted to validate the brain-targeting efficacy of the Tf-Rutin liposomes. A susceptible detection method was developed to characterize the pharmacokinetics of Tf-Rutin-Lip further. The optimized conditions for the preparation of Tf-Rutin-Lip were determined as follows: a lipid-to-cholesterol ratio of 4.63:1, a drug-to-lipid ratio of 1:45.84, a preparation temperature of 42.7 °C, a hydration volume of 20 mL, a sonication time of 10 min, a surfactant concentration of 80 mg/mL, a DSPE-MPEG-2000 concentration of 5%, and a DSPE-PEG2000-COOH to DSPE-MPEG-2000 molar ratio of 10%. The liposomes did not affect the cell activity of bEnd.3 cells at 24 h and did not disrupt the tight junction of the blood–brain barrier. Tf-modified liposomes were taken up by bEnd.3 cells, which, in turn, passed through the BBB, thus improving liposomal brain targeting. Furthermore, the results of pharmacokinetic experiments showed that the Cmax, AUC0-∞, AUC0-t, MRT0-∞, and t1/2of Tf-Rutin-Lip increased 1.99-fold, 2.77-fold, 2.58-fold, 1.26-fold, and 1.19-fold compared to those of free Rutin solution, respectively. These findings suggest that Tf-Rutin-Lip is brain-targeted and may enhance the efficacy of Rutin in the treatment of brain disorders.
研究领域:
药剂学
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神经科学
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药物递送系统
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药理学
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PubMed
高影响因子
FOOD HYDROCOLLOIDS
·
2025
·
IF 12.4
Shupin Ye,Yuteng Fu,Fangjian Ning,Jianyong Wu,Shunjing Luo,Jiangping Ye,Chengmei Liu
Polyphenolic compounds demonstrate significant potential in inhibiting starch digestion. However, the poor solubility, poor reactivity and easy degradation of polyphenols limit their effectiveness on starch digestion. The present study aims to avoid polyphenolic limitation in an effective strategy with the utility of rutin nanoparticles, and to co-gelatinize them with rice starch for regulating starch digestibility. SEM and XRD analysis had shown that rutin existed in an amorphous and non-inclusion state within NPS sample. It was accompanied by the absorption peak intensity related to hydrogen bonding weakened and shifted to lower wavenumbers in FTIR analysis. The double-helical domain formation increased from 4.57 % to 6.13 % in 13 CP/MAS NMR analysis. In-vitro digestion study showed that rutin nanoparticles exhibited maximum inhibition of starch digestion. Rutin was gradually released from NPS sample during digestion, with the highest concentration of rutin in its digestive fluid, showing the highest inhibition for digestive enzyme activity with values decreasing from 0.178 U/mL to 0.081 U/mL. Therefore, rutin nanoparticles can significantly regulate starch digestion by altering rice starch structural domains and inhibiting digestive enzyme activity. Overall, this study established a novel strategy to inhibit starch digestion by polyphenol nanoparticles, offering potential applications in low-glycemic starchy foods.
研究领域:
食品科学
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生物化学
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高影响因子
Foods
·
2022
·Vol. 11
·期 18
·2827
·
IF 5.561
Cuicui Li,Long Chen,David Julian McClements,Xinwen Peng,Chao Qiu,Jie Long,Hangyan Ji,Jianwei Zhao,Xing Zhou,Zhengyu Jin
In this work, rutin (RT)–loaded zein–carboxymethyl starch (CMS) nanoparticles were successfully prepared by the antisolvent precipitation method. The effect of CMS on composite nanoparticles at different concentrations was studied. When the ratio of zein–RT–CMS was 10:1:30, the encapsulation efficiency (EE) was the highest, reaching 73.5%. At this ratio, the size of the composite nanoparticles was 196.47 nm, and the PDI was 0.13, showing excellent dispersibility. The results of fluorescence spectroscopy, FTIR, XRD, and CD showed that electrostatic interaction, hydrogen bonding, and hydrophobic interaction were the main driving forces for the formation of nanoparticles. It can be seen from the FE–SEM images that the zein–RT–CMS nanoparticles were spherical. With the increase in the CMS concentration, the particles gradually embedded in the cross–linked network of CMS (10:1:50). After RT was loaded on zein–CMS nanoparticles, the thermal stability and pH stability of RT were improved. The results showed that zein–CMS was an excellent encapsulation material for bioactive substances.Keywords:zein;carboxymethyl starch;rutin;nanoparticle;interaction;formation mechanism
研究领域:
纳米技术
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药学
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材料科学
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PubMed
高影响因子
CELL BIOLOGY AND TOXICOLOGY
·
2024
·Vol. 40
·期 1
·1-25
·
IF 5.3
Wu Wentong,Li Jinjin,Yin Yiming,Zhou Yourong,Huang Xiangliang,Cao Yashi,Chen Xueqin,Zhou Yunfang,Du Jiangxia,Xu Zhifei,Yang Bo,He Qiaojun,Yang Xiaochun,Hu Yuhuai,Yan Hao,Luo Peihua
Ensartinib, an approved ALK inhibitor, is used as a first-line therapy for advanced ALK-positive non-small cell lung cancer in China. However, the hepatotoxicity of ensartinib seriously limits its clinical application and the regulatory mechanism is still elusive. Here, through transcriptome analysis we found that transcriptional activation of TXNIP was the main cause of ensartinib-induced liver dysfunction. A high TXNIP level and abnormal TXNIP translocation severely impaired hepatic function via mitochondrial dysfunction and hepatocyte apoptosis, and TXNIP deficiency attenuated hepatocyte apoptosis under ensartinib treatment. The increase in TXNIP induced by ensartinib is related to AKT inhibition and is mediated by MondoA. Through screening potential TXNIP inhibitors, we found that the natural polyphenolic flavonoid rutin, unlike most reported TXNIP inhibitors can inhibit TXNIP by binding to TXNIP and partially promoting its proteasomal degradation. Further studies showed rutin can attenuate the hepatotoxicity of ensartinib without antagonizing its antitumor effects. Accordingly, we suggest that TXNIP is the key cause of ensartinib-induced hepatotoxicity and rutin is a potential clinically safe and feasible therapeutic strategy for TXNIP intervention. Graphical Abstract
研究领域:
肿瘤学
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药理学
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分子生物学
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细胞生物学
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PubMed
高影响因子
INORGANIC CHEMISTRY COMMUNICATIONS
·
2025
·
IF 5.4
Wanwan Li,Duo Qian,Shunchang Liu,Ziyi Huang,Zhenwei Huang,Danrong Chen,Jinhua Xue,Shengyuan Yang
Herein, a novel type of nitrogen and sulfur co-doped dual-emission carbon dots (N, S-CDs) was successfully synthesized through a one-step hydrothermal method, utilizing o-diaminobenzene and sulfosalicylic acid as precursors. The prepared N, S-CDs exhibited two distinct emission peaks located at 345 nm and 435 nm when excited at 305 nm. Notably, rutin (RT) can selectively quench the emission of N, S-CDs at 345 nm via fluorescence resonance energy transfer (FRET) mechanism, while the fluorescence intensity at 435 nm is essentially stable. As a result, the fluorescence intensity ratio of the dual emission wavelengths at 345 nm and 435 nm gradually decreased with the increase of RT concentration. There was a good linear relationship between the change in fluorescence intensity ratio ( F 0 F ) /F 0 and the concentration of RT in the range of 0.5–60 μM, and the limit of detection was 0.14 μM. Furthermore, the ratiometric fluorescent sensor based on N, S-CDs proved to be highly effective for detecting RT in real samples such as human blood serum, urine, and RT tablet, achieving satisfactory recovery rates and demonstrating broad potential for future applications.
研究领域:
分析化学
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纳米材料
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生物传感器
·
荧光传感
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高影响因子
危险品化学品经营许可证（带存储）