TALANTA
·
2025
·
IF 6.1
Mengqiu Xiong,Yao Wang,Shuhan Lu,Nasifu Lubanga,Tao Li,Zhihao Li,Bangshun He,Ying Li
Multiple infections of respiratory viruses are common in patients with clinical respiratory diseases, but current detection methods still have problems such as complex equipment and long detection time. Rapid, low-cost, and on-site detection of human respiratory viruses is crucial for both clinical diagnosis and population screening. In this research, we created a space-coded microfluidic chip (SC-Chip) for the recognition of nine respiratory viruses: influenza A virus, influenza B virus, severe acute respiratory syndrome coronavirus 2, human coronavirus OC43, human coronavirus NL63, human coronavirus HKU1, human respiratory syncytial virus, human parainfluenza virus, and human metapneumovirus. For the first time, a comprehensive sequence comparison among these viruses was performed to design the recombinase polymerase amplification (RPA) primers and Cas12a-crRNAs. The SC-Chip partitions samples amplified by RPA into spatially coded wells preloaded with CRISPR-Cas12a detection reagents, enabling the identification of all nine viral targets in a single test using a single fluorescence probe. The chip-based assay displays 9 respiratory viruses in less than 40 min with a minimum detection limit at a concentration of 10 −18 M (∼1 copy/reaction). Additionally, the efficacy of the method was assessed through its application to 35 clinical patient samples identified as being at risk for respiratory virus infection, yielding a sensitivity of 90 % and a specificity of 100 %. In summary, this space-coded microfluidic CRISPR system offers several advantages, including ease of operation, cost-effectiveness, and rapid data acquisition, thereby holding great potential for multiplexed detection of nucleic acid targets in a clinical setting.
研究领域:
分子生物学
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医学诊断
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微流控技术
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产品关联
DOI
PubMed
高影响因子
ADVANCED ENGINEERING MATERIALS
·
2024
·
IF 3.4
Shuang Huang,Chuanjie Yao,Mengyi He,Xinshuo Huang,Zhengjie Liu,Jiayi Chen,Lelun Jiang,Hui-jiuan Chen,Xi Xie
Metabolic substances play a pivotal role in maintaining the body's regular physiological functions. When these processes are disrupted, it can lead to metabolic disorders which may cause severe damage to various organs. Diabetes mellitus, a prevalent metabolic disorder, arises from disturbances in sugar metabolism among other substances. Consequently, there's a pressing need to monitor metabolite levels for early diagnosis. To address this, in this study, a semi-implantable metabolite sensing system developed around functionalized microwires-based bundle electrodes (FMBE) is introduced. This FMBE device tracks glucose, hydrogen peroxide (H 2 O 2 ), and uric acid (UA) levels in vivo in real time, sensitively, and continuously. The FMBE is coated with Au nanoclusters and carbon nanotube–2D carbides and nitride (MXene) nanocomposites to enhance sensing surface area. In vitro characterizations affirm the FMBE's linear responsivity, detection sensitivity, and selectivity toward glucose, H 2 O 2 , and UA sensing. In addition, in in vivo testing in healthy and diabetic rats, it is demonstrated that FMBE is able to continuously monitor interstitial glucose, H 2 O 2 , and UA concentrations after implantation. The FMBE system, thus, stands out as a promising platform for real-time, in situ monitoring of metabolite concentrations, potentially assisting in the diagnosis of diabetes and associated complications.
研究领域:
生物医学工程
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分析化学
·
糖尿病研究
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产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Yuping Wang,Yiting Lei,Nan Wang,Jun Zhang,Wenguo Cui,Xiaoji Luo
The challenge of achieving graded regeneration of adjacent tissues remains significant in tissue repair and regeneration. For instance, the disruption of the microenvironment caused by “osteogenesis mobilization” often results in fibrosis of the regenerated cartilage, ultimately leading to osteoarthritis. Inspired by the utilization of activated carbon for adsorbing and filtering small particles in air purification and water treatment, we have designed an integrated hydrogel scaffold capable of absorbing the endogenous BMP-2 produced by “osteogenesis mobilization”, confining it to the bone defect area, and preventing its diffusion into the cartilage region, thereby facilitating physiological osteochondral gradient regeneration. The osteogenic layer of this integrated scaffold is NHAP-AHAMA-B2A, which captures the BMP-2 produced by “osteogenesis mobilization” and amplifies its osteogenic effect through the grafted peptide B2A. This upregulation enhances the expression of ALP, OCN, and OPN in MSCs within the osteogenic layer, promoting osteogenic differentiation. The chondrogenic layer, HAMA-B2A, utilizes the peptide B2A to upregulate the expression of COL II and ACAN in MSCs, fostering chondrogenic differentiation. This integrated HAMA-B2A/NHAP-AHAMA-B2A hydrogel scaffold confines the BMP-2 produced by “osteogenesis mobilization” to the bone defect area, prevents its diffusion into the cartilage defect area, and effectively utilizes it to induce stem cell osteogenic differentiation. Simultaneously, it induces stem cell chondrogenic differentiation in the cartilage defect area, achieving transparent cartilage regeneration. In summary, the osteogenic and chondrogenic layers of this integrated scaffold collaborate to achieve a physicochemical gradient regeneration of bone-cartilage.
研究领域:
组织工程
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再生医学
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生物材料科学
·
骨科
✓ 已验证
产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Xiaoxiang Zhou,Jiarong Liu,Yueran Hua,Yi Jia,Tianyi Zhang,Rui Zhang,Hua Wang,Hongxing Shen,Jinfeng Xu,Min Li
Bacterial resistance is a serious threat to global public health, creating a heavy burden on healthcare systems and patients. Traditional antimicrobial susceptibility testing (AST) is time-consuming, and two-dimensional (2D) culture systems fail to accurately replicate the three-dimensional (3D) microenvironment in which bacteria naturally grow. Moreover, conventional AST method only provide endpoint susceptibility results and cannot continuously monitor drug-bacteria interactions. In this study, we used a microfluidic chip to produce monodisperse alginate hydrogel microspheres, which were then used to isolate and encapsulate single Escherichia coli ( E. coli ) strain for 3D culture and AST. Our results demonstrate that the structure and limited space inside the microspheres provide a microenvironment similar to the natural 3D conditions for bacterial growth. Using this hydrogel microsphere-based 3D culture system, we performed rapid minimum inhibitory concentration (MIC) testing and continuous monitoring of E. coli strain to 3 antibiotics with different action mechanisms. This system enables to offer MIC results within 6 h, much faster than conventional methods (>16 h). We believe that combining microfluidic technology with 3D microsphere confined culture can not only effectively meet the needs of rapid clinical diagnosis and treatment by shortening the time needed for MIC testing, but also capture dynamic bacterial growth and antibiotic interactions, providing more valuable information for studying resistance mechanisms and developing treatment strategies.
研究领域:
微生物学
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生物医学工程
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抗生素耐药性
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芯片实验室技术
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传染病诊断
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF SEPARATION SCIENCE
·
2014
·Vol. 38
·期 2
·346-355
·
IF 2.594
Teng Chen,Xingchu Gong,Huali Chen,Haibin Qu
A systematic, yet simple method for the decoloration of Panax notoginseng extracts has been developed by static adsorption tests and response surface methodology. Through static adsorption experiment screening, acidic alumina was selected because of its high decoloration ratio and saponin recovery ratios. Using response surface methodology, the correlation between the process parameters (i.e., sample volume and flow rate) and decoloration performance was modeled. A design space of the decoloration process was subsequently established through the proposed models. The verification experimental values were in good agreement with the predicted values. The design space was proven reliable, because all the verification experimental results attained the criteria for design space development. Moreover, most of the saponins adsorbed by the acidic alumina could be recovered through dynamic desorption using water and ethanol. The method developed in the current study is highly efficient, flexible, and easy to control, thus providing a promising approach for the decoloration of Panax notoginseng extracts with consistent decoloration performance.
研究领域:
药学
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化学工程
·
分析化学
✓ 已验证
DOI
PubMed
高影响因子
MICROPOROUS AND MESOPOROUS MATERIALS
·
2017
·
IF 3.615
Shuo Tao,Xiaolei Li,Huimin Gong,Qike Jiang,Wenguang Yu,Huaijun Ma,Renshun Xu,Zhijian Tian
Hierarchical MgAPO-11 molecular sieves with large BET surface areas and high pore volumes have been synthesized via dry-gel conversion of the magnesioaluminophosphate-carbon (MAP-C) composites. The carbon film coated on the mesopores of amorphous magnesioaluminophosphate (MAP) provides a confined-space to restrict the overgrowth of the crystals. The products were well characterized using XRD , SEM, TEM , N 2 adsorption-desorption, infrared spectroscopy of pyridine adsorption (Py-IR) and solid-state NMR techniques. The results show that highly mesoporous MgAPO-11 with good crystallinity can be obtained at an appropriate carbon loading amount. The time-dependent study indicates that the formation process of hierarchical MgAPO-11 obeys a reversed crystal growth route: crystallization started on the outer surface of the amorphous precursor, then extended to inward, and finally hierarchical molecular sieves assembled by nanosized crystals with a size of about 50 nm were obtained. The catalyst based on hierarchical MgAPO-11 exhibits a significant higher activity and selectivity than the conventional Pt/MgAPO-11 catalyst in the hydroisomerization of n -dodecane, attributing to its higher acidity and excellent diffusion properties resulting from the hierarchical pore architecture.
研究领域:
材料科学
·
催化化学
✓ 已验证
产品关联
DOI
高影响因子
Science China-Chemistry
·
2016
·Vol. 59
·期 7
·824-829
·
IF 2.429
Zhao Jianwei,Sun Yinlu,Gao Yajun
The diffusion behavior of particles in the chromatography is a fundamental issue of chromatographic dynamics. The understanding of the diffusion behaviors is particularly critical to optimize the operation conditions, improve the chromatographic performance and design a new separation device. Many of the present simulations focus on chromatographic thermodynamics, and very few aim at the overall diffusion and separation process. In order to dynamically trace the trajectory of the diffusing particles and to perform simulations of the whole chromatographic process, we have developed a model based on the framework of random walk in the restricted space and performed the simulation of a single particle diffusion in the gas chromatography. The simulation parameters were determined by comparing with the experimental data. The elution profiles of n-alkanes under different flow rates were accurately simulated with the method. The results show that the relative difference between the measures and the simulations are less than 2% and 10% for the retention time and the peak width, respectively. The simulation method shows great significance for the optimization of separation conditions and the development of novel technologies of chromatographic separation.
研究领域:
分析化学
·
物理化学
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2021
·
IF 6.986
Yuling Wang,Rongrong Pan,Depeng Jiang,Dechen Jiang,Hong-Yuan Chen
The chemical reaction in a confined space is known to be accelerated due to a high collision probability; however, the study of this confinement effect in a supersmall space down to femtoliter (fL) is seldom reported. Here, an adjustable volume [from picoliter (pL) to fL] of the aqueous phase is retrained at the tip of a nanopipette by an organic solvent so that the confinement effect on the specific activity of glucose oxidase is investigated. The activity is determined by the amount of hydrogen peroxide generated from the reaction between the oxidase and glucose using a nanoelectrode inside the nanopipette. As compared with the activity in bulk solution (82 U/mg), the activity increases up to 7500 U/mg in a 105 fL space. The 2 orders of magnitude increase in the enzymatic activity is the highest amplification in the volume-confined enzyme reaction as reported. A near-exponential drop in the activity is observed with the increase in the space volume, revealing the dominant enhancement in the confined space at the fL level for the first time. The established electrochemical nanopipettes should not only provide a strategy for the study of the enzymatic activity in supersmall confined space but also help understand the confinement effect of enzyme-catalyzed reactions.
研究领域:
生物化学
·
电化学
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2022
·
IF 16.744
Zhen Zhang,Yu-e Shi,Yibo Liu,Yifei Xing,Ding Yi,Zhenguang Wang,Dongpeng Yan
Efficient blue and deep-blue room temperature phosphorescence (RTP) is still a great challenge due to the difficulties in populating and stabilizing the excitons in relatively high-lying states. Inorganic boric acid , lacking conventional luminophores in its structure, is generally not considered as efficient material for RTP. In this work, we illustrated how to make deep-blue RTP materials with both high emission efficiency (phosphorescence quantum yield of 45%) and an ultralong lifetime (2.9 s) through a heat treatment strategy. Moreover, a through-space conjugation assisted energy transfer and rigidification mechanism was proposed for explaining the simultaneous promoting of emission lifetime and efficiency. In addition, the deep-blue RTP materials displayed excellent stability against restrict external environments (such as high concentration acid, alkali, oxidant, reductant and organic solvents). Benefiting from these high-performance RTP materials, multi-level information encryption under harsh conditions was demonstrated. The research results thus provide a universal principle and strategy for designing stable and efficient blue RTP materials, which also extend their applications in various environments.
研究领域:
材料科学
·
光学
·
化学
✓ 已验证
产品关联
DOI
高影响因子
APPLIED THERMAL ENGINEERING
·
2022
·
IF 6.465
Ying Xu,Kai Sheng,Hongyin Xu,Junfeng Li,Wei Wang
Recently, solar-driven interfacial distillation has rapidly developed into an effective technology for unconventional water treatment. Solar evaporator as the central part of the system was extensively investigated and achieved high light-to-heat efficiencies. However, the combination of interfacial evaporators and solar still in the application was rarely studied, and the phenomena inside the device during evaporation have yet to be revealed. Here, we designed an interfacial evaporator with a conical solar still unit to study the enhancement of water collection. The physical changes inside the device during natural solar irradiation were also revealed. In addition, the COMSOL Multiphysics as simulation software was applied to reveal the temperature variation of the system during solar motion. As a result, the condensate collection of the space-occupied 3D system is 2.5 times that of the plant system in the actual solar irradiation, which is even larger than the laboratory irradiation process. In addition to more solar energy absorption during the solar movement, the faster saturation of the humidity (more than 90%) due to the space occupation is also an important factor for the higher water collection capacity of the 3D system. This discovery would provide a reference for the design of the interfacial evaporator and evaporation device in the future and promote its practical application.
研究领域:
材料科学
·
环境工程
✓ 已验证
产品关联
DOI
高影响因子
PROCESS SAFETY AND ENVIRONMENTAL PROTECTION
·
2023
·
IF 7.8
Andong Yu,Juan Zhou,Min Hua,Xuhai Pan,Juncheng Jiang,Sanming Wang
The process of styrene polymerization is a typical hazardous chemical process. Due to its high reaction temperature and high product viscosity , it is very prone to thermal runaway accidents. In order to reduce the occurrence of such accidents, this work investigates the thermal hazard of the polymerization process by reaction calorimetry experiments and deduces the thermal runaway criterion for the styrene polymerization reaction by combining the results of gas chromatography experiments. The phase space of the system was reconstructed by the delayed coordinate method to obtain the reconstructed divergence criterion ( re-div ). The results showed that the exothermic heat and adiabatic temperature rise of the polymerization reaction showed a logarithmic increase with the increase of the initial reaction temperature. Compared with the div calculated by numerical method and analytical method, the analytical method div is more accurate and effective. The analytical divergence criterion ( div ) curve is strictly decreasing in the post-polymerization case, which indicates that it can be used as an off-line criterion to determine the safety of the reaction process by the initial reaction condition parameters. After the experimental verification, it can be confirmed that the re-div can predict whether the reaction is out of control in advance and accurately.
研究领域:
化学工程
·
安全科学
✓ 已验证
产品关联
DOI
高影响因子
Journal of Materials Chemistry C
·
2022
·Vol. 10
·期 47
·18025-18032
·
IF 8.067
Shu-Yan Wang,Xiao-Juan Zhen,Zhan-Zu Feng,Ming-Jun Xiao,Shi-Ji Li,Wen Shang,Yi-Fan Huang,Qiang Wang,Hao-Li Zhang
Space exploration urgently demands novel irradiation-tolerant materials to build the arsenal for outer space use, among which nonlinear optical materials and devices are ever-increasing attractive. Tin sulfide (SnS), a novel two dimensional (2D) BP-analog with enhanced stability under ambient conditions, has exhibited excellent optoelectronic properties on the ground, but its potential as a candidate for space optics remains to be explored. Herein, the spatial adaptability of SnS was evaluated in the simulated space radiation environment. When exposed to 60Co γ-rays at a dose equal to over 45 year accumulation in a typical earth orbit, SnS still maintained its strong third-order nonlinear saturable absorption ranging from visible to near-infrared spectra. Ultrafast spectroscopy is used to study the radiation effects and relevant damage mechanisms that are difficult to be revealed via conventional techniques. This work not only sheds light on the irradiation-induced ionization damage and defects in SnS from the unique perspective of ultrafast excited state dynamics but also provides general guidance for rational design and screening space radiation-tolerant nonlinear optical materials.
研究领域:
材料科学
·
光学
·
空间科学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）