Journal of Environmental Chemical Engineering
·
2026
·Vol. 14
·期 3
·122797
·
IF 7.5
Huanlong Liu,Jie Zhao,Shen Tang,Wanxin Cheng,Yanhong Li,Dunqiu Wang,Jie Zhang
The co-contamination of hexavalent chromium Cr(VI) and chlorpyrifos (CPF) poses a severe challenge to traditional remediation technologies due to synergistic toxicity. This study constructed a bio-material synergistic system (FeO/C@ S. oneidensis MR-1) for their simultaneous removal. Results demonstrated that under optimal conditions (0.08 g/L FeO/C, 10% amount of inoculation, pH 7.0), the removal rates of Cr(VI) and CPF reached 75.5% and 93.7%, respectively, maintaining robust degradation efficiency even under a severe organic shock load of 50 mg/L CPF. Importantly, comprehensive multi-scale characterizations (SEM, CLSM, EDS, XRD, FTIR, XPS, 3D-EEM), revealed a bidirectional bio-material synergy: FeO/C acted as an electron shuttle and play a role of bio-driven electron allocation, preferentially driving the Fe(III)/Fe(II) cycle to detoxify Cr(VI), while dynamically secreted extracellular polymeric substances (EPS) acted as an active interface to enrich CPF ensuring the efficient biomineralization of CPF and complex heavy metals. This dual action effectively alleviated acute oxidative stress, enabling cell viability to recover to 80%. For real-world applications, a clear takeaway is that this self-sustaining system entirely eliminates the need for external chemical electron donors. It offers a scalable, bio-driven electron allocation green engineering strategy for the in-situ bioremediation of complex heavy metal-organic wastewater.
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Surfaces and Interfaces
·
2023
·
IF 6.2
Diandi Li,Haijun He,Zhongxuan Xu,Hong Deng
Coexistence of chromium and iron, manganese and copper in industries is common, which may affect the microbial treating efficiency and toxicity to microorganisms, thus restricting the application of microbial treatment technology. In this research, Cu 2+ promoted the reduction of Cr(VI) and the fixation of total chromium in bimetallic systems for both Shewanella oneidensis MR-1 and Bacillus subtilis , while Mn 2+ and Fe 3+ exhibited inhibition effects. Generally, groups with coexisting heavy metal of 0.1 mM were most affected, no matter promotive or inhibitive. Toxicity analysis revealed that high concentration of heavy metal ions led to damage to the cell membranes and reduction of their survival rate. To clarify the optimal condition for microbial treatment, response surface methodology was used to investigate the influencing factors of Cr(VI) reduction by S. oneidensis . The total chromium concentration in the supernatant was correlated with three factors, and the significant order of influence was: culture time > culture temperature > pH.
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CHEMICAL ENGINEERING JOURNAL
·
2022
·
IF 16.744
Cholnam Ri,Fengxiang Li,Hyokchol Mun,Linan Liu,Jingchun Tang
The technology of integrating sulfidated zero-valent iron and functional anaerobic bacteria has been extensively explored to improve the removal efficiency of pollutants and enhance the performance of anaerobic microbial system. In this study, the simultaneous DCNB(2,4-dichloronitrobenzene) reduction and methane production by ball-milled 1:1 Fe 0 /FeS 2 (briefly, 1:1 Fe 0 /FeS 2 ), Shewanella oneidensis MR-1 and microbial consortium (MC) were firstly investigated. The result showed that the DCNB reduction was increased by 2.6 and 1.7 times in 1:1 Fe 0 /FeS 2 + MR-1 group compared to 1:1 Fe 0 /FeS 2 and MR-1, respectively, while it further increased by almost 2 times in 1:1 Fe 0 /FeS 2 + MC + MR-1 group as compared to that of 1:1 Fe 0 /FeS 2 + MR-1 group. Moreover, the methane production was drastically facilitated via the reciprocal interaction among FeS as the reaction product, MR-1 and MC. Dechlorination was not found, mainly due to the inability of 1:1 Fe 0 /FeS 2 and the inexistence of dechlorinating bacteria in original inoculum. Meanwhile, the microbial community was also changed, with genera like Eubacterium capable of reducing nitroaromatic compound and Methanosarcina as acetoclastic methanogen enriched by 1:1 Fe 0 /FeS 2 and MR-1. Therefore, fast DCNB reduction and methane production using 1:1 Fe 0 /FeS 2 in anaerobic microbial system, gives useful reference for both establishment of pollutant removal and biogas production. In conclusion, Fe 0 /FeS 2 , as a kind of sulfidated ZVI, is a good candidate for enhancing both pollutant degradation and CH 4 production in anaerobic wastewater treatment.
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Journal of Environmental Chemical Engineering
·
2025
·
IF 7.2
Jie Zhang,Ting Hu,Shen Tang,Yanhong Li,Huanlong Liu
This study elucidates the mechanism by which hexavalent chromium [Cr(VI)] synergistically promotes malathion biodegradation in Shewanella oneidensis MR-1. Exposure to 5 mg/L Cr(VI) significantly enhanced the malathion degradation rate by 2.3-fold, achieving 96.79 % removal within 72 h under optimized conditions (5 % inoculum, pH 6). Analysis of cell membrane parameters combined with microscopic observation techniques indicated that Cr(VI) induced biofilm densification and membrane lipid peroxidation. Metabolomic profiling identified three critical Cr(VI)-activated pathways: (1)tryptophan-mediated redox homeostasis, (2)sulfur assimilation for antioxidant substances biosynthesis, and (3)cofactor biosynthesis for electron transport optimization. These metabolic adaptations increased membrane permeability and accelerated reactive oxygen species diffusion. The findings highlight Cr(VI) as a metabolic modulator that reprograms microbial catabolism via redox signaling, providing a mechanistic basis for developing biocatalysis strategies in multi-pollutant environments.
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CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Shuyu He,Shaohua Chen,Liwen Zhang,Shanyi Tan,Huizi Wen,Luyao Xi,Xiangru Zuo,Dongyun Du
Dissimilatory iron-reducing bacteria (DIRB) offer an eco-friendly approach to address nanoscale zero-valent iron (nZVI) surface passivation problem, yet their efficacy is limited by the slow direct extracellular electron transfer (EET) between DIRB and Fe(III) oxide passivation layers (Fe( III )OPL). This study demonstrates that natural dissolved humic substances (DHS)-mediated electron shuttling unlocking nZVI reactivity in Shewanella oneidensis MR-1 (MR-1) system for enhance Cr(VI) removal. Seven DHSs from diverse sources were evaluated, revealing that Cr(VI) removal rate constants ( k obs ) correlate strongly with DHS's quinone content (C=O) and electron transfer capacity (ETC), quantified as k obs = 1.66 C C = O − 9.39 ( P < 0.0007) and k obs = 19.37 ETC + 0.74 ( P < 0.0005). DHS-mediated systems achieve 2.0–12.1-fold higher k obs than nZVI/MR-1 alone. The enhancement mechanism of DHS-mediated Cr(VI) removal by nZVI/MR-1 is that DHS effectively facilitates Fe( III )OPL dissolution on the nZVI surface via electron shuttling, maintaining nZVI reactivity and amplifying Fe(II) generation by 1.65-fold. Moreover, DHS stimulates MR-1 to secrete more extracellular polymeric substances (EPS), enhancing Cr(VI) adsorption and reduction. This work elucidates DHS's dual role in maintaining nZVI activity and amplifying Cr(VI) removal by nZVI/MR-1, while establishing a predictive framework linking DHS molecular traits parameters to Cr(VI) removal kinetic, offering sustainable strategies for heavy metal-contaminated water bodies remediation by integrating biogeochemical and material engineering principles.
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IET Nanobiotechnology
·
2018
·Vol. 12
·期 4
·441-445
·
IF 2.059
Xiaojie Song,Xianyang Shi,Mi Yang
Biosynthesised nanocomposites have attracted growing interests attributed to their ‘green’ synthesis nature in recent years. Shewanella oneidensis MR-1, a dissimilatory metal-reducing bacterium, was used to reduce palladium (II) nitrate to palladium (0) nanoparticles (Pd NPs) under anaerobic conditions, resulting in the in situ formation of Pd NPs immobilised on TiO 2 nanotubes (TNTs) (Pd/TNTs nanocomposites). The Pd/TNTs nanocomposites were characterised by transmission electron microscopy, X-ray diffraction, X-ray photoelectron spectroscopy, energy dispersive X-ray, and electron spin resonance, respectively. The results indicated that Pd NPs are successfully grown on the TNTs without aggregation. Photocatalytic degradation of methylene blue (MB) by Pd/TNTs nanocomposites under simulated sunlight was also investigated. Pd/TNTs nanocomposites had photocatalytic efficiency superior to that of single TiO 2 nanomaterials. The photocatalytic activity of Pd/TNTs nanocomposites can be enhanced by S. oneidensis MR-1. The results showed that after only 10 min, the degradation ratio of MB reached 98.7% by Pd/TNTs nanocomposites when simultaneously assisted with S. oneidensis MR-1.
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JOURNAL OF HAZARDOUS MATERIALS
·
2026
·
IF 10.6
Jing Sun,Hong Shu,Xiaoyan Wu,Tingting Liu,Guangyue Li,Zhao Cui,Ting Li,Xiaowei Zhou,Zhongran Dai,Qian Li
Large quantities of low-concentration uranium-contaminated water from the nuclear industry poses a critical environmental challenge due to the limitations of current methods on removal efficiency and adsorption selectivity. This study developed a novel biofilm cathode for enhanced uranium adsorption by leveraging the quorum sensing (QS) system of Shewanella oneidensis MR-1. Biofilm formation was induced using exogenous acyl-homoserine lactones (AHLs), with C4-HSL (10 μmol/L) identified as the most efficient signal molecule. It significantly improved biofilm properties, increasing thickness by 107.7 % versus the control without AHLs, boosting extracellular protein content, and increasing the proportion of living cells. Microstructure analysis (CLSM) revealed a “base-polysaccharide-gel” three-dimensional structure, where proteins, lipids, and β-polysaccharides form a functional gel layer that provides the primary functional matrix for uranium binding. The C4-HSL-induced biofilm exhibited a 43.4 % higher reduction peak current and a 33.3 % decrease in electron transfer resistance, confirming improved electron transfer efficiency. Furthermore, coupling CLSM, SEM-EDS, FT-IR and XPS analysis indicated uranium capture was primarily dominated by complexation/coordination with functional groups on extracellular polymeric substances (EPS), supplemented by electrochemical reduction of 25.91 % soluble U(VI) to insoluble U(IV). Accordingly, a possible mechanism model of QS-driven biofilm cathode is proposed for optimizing biofilm structure and enhancing uranium capture. Finally, verification tests in actual uranium-contaminated groundwater demonstrated the biofilm cathode exhibited exceptional performance with a 99.4 % uranium recovery rate and high selectivity (K d,U at 34.57 L/g). These findings highlight the significant potential of AHLs-triggered QS as a powerful strategy to optimize bioelectrochemical properties for highly-efficient uranium remediation and resource recovery.
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ENVIRONMENTAL SCIENCE & TECHNOLOGY
·
2022
·
IF 11.357
Ruixia Han,Zhe Wang,Jitao Lv,Zhe Zhu,Guang-Hui Yu,Gang Li,Yong-Guan Zhu
Microbially mediated iron redox processes are of great significance in the biogeochemical cycles of elements, which are often coupled with soil organic matter (SOM) in the environment. Although the influences of SOM fractions on individual reduction or oxidation processes have been studied extensively, a comprehensive understanding is still lacking. Here, using ferrihydrite, Shewanella oneidensis MR-1, and operationally defined SOM components including fulvic acid (FA), humic acid (HA), and humin (HM) extracted from black soil and peat, we explored the SOM-mediated microbial iron reduction and hydroxyl radical (•OH) production processes. The results showed that the addition of SOM inhibited the transformation of ferrihydrite to highly crystalline iron oxides. Although FA and HA increased Fe(II) production over four times on average due to complexation and their high electron exchange capacities, HA inhibited 30–43% of the •OH yield, while FA had no significant influence on it. Superoxide (O2•–) was the predominant intermediate in •OH production in the FA-containing system, while one- and two-electron transfer processes were concurrent in HA- and HM-containing systems. These findings provide deep insights into the multiple mechanisms of SOM in regulating microbially mediated iron redox processes and •OH production.
研究领域:
环境科学
·
微生物学
·
地球化学
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SCIENCE OF THE TOTAL ENVIRONMENT
·
2024
·
IF 8.2
Lanlan Zhang,Shanshan Chen,Yuting Yang,Shuyi Xie,Lijuan Luo,Yaobin Lu,Tiangang Luan
With the death and decomposition of widely distributed photosynthetic organisms, free natural pigments are often detected in surface water, sediment and soil. Whether free pigments can act as photosensitizers to drive biophotoelectrochemical metabolism in nonphotosynthetic microorganisms has not been reported. In this work, we provide direct evidence for the photoelectrophic relationship between extracellular chlorophyll a (Chl a ) and nonphotosynthetic microorganisms. The results show that 10 μg of Chl a can produce significant photoelectrons (∼0.34 A/cm 2 ) upon irradiation to drive nitrate reduction in Shewanella oneidensis . Chl a undergoes structural changes during the photoelectric process, thus the ability of Chl a to generate a photocurrent decreases gradually with increasing illumination time. These changes are greater in the presence of microorganisms than in the absence of microorganisms. Photoelectron transport from Chl a to S. oneidensis occurs through a direct pathway involving the cytochromes MtrA, MtrB, MtrC and CymA but not through an indirect pathway involving riboflavin. These findings reveal a novel photoelectrotrophic linkage between natural photosynthetic pigments and nonphototrophic microorganisms, which has important implications for the biogeochemical cycle of nitrogen in various natural environments where Chl a is distributed.
研究领域:
微生物学
·
环境科学
·
生物地球化学
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