Journal of Nanomaterials
·
2018
·Vol. 2018
·期 1
·1634814
·
IF 2.207
Lianbing Zhong,Guihong Geng,Yujin Wang,Feng Ye,Limeng Liu
A fully dense TaC ceramic was prepared by hot pressing using 10 mol% ZrC plus 5 mol% Cu as a sintering aid. Formation of (Ta, Zr)C solid solution (ss) by reaction between TaC and ZrC facilitated densification. Addition of Cu refined the microstructure and consequently improved flexural strength of the TaC ceramics. TEM investigation found ubiquitous precipitation of nanocrystallites at multigrain conjunctions. The nanocrystallites were (Ta, Zr)C solid solution with uniform dispersion in an oxygen-rich glassy matrix. Although formation of nanoprecipitates may not much affect the mechanical properties of the TaC ceramic, the structure suggested a new type of nanoceramic worth further research.
研究领域:
材料科学
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陶瓷学
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固态化学
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显微结构分析
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复合材料
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DOI
高影响因子
International Journal of Biological Sciences
·
2022
·Vol. 18
·期 16
·6008-6019
·
IF 10.75
Li Chao,Lv Li-fang,Qi-Li Mu-ge,Yang Rui,Wang Yu-jing,Chen Shuang-shuang,Zhang Ming-xiu,Li Tian-yu,Yu Tong,Zhou Yu-hong,Liang Hai-hai,Shan Hong-li,Li Xue-lian
Cardiac fibrosis is one of the common pathological processes in many cardiovascular diseases characterized by excessive extracellular matrix deposition. SerpinE2 is a kind of protein that inhibits peptidase in extracellular matrix and up-regulated tremendously in mouse model of cardiac fibrosis induced by pressure-overloaded via transverse aortic constriction (TAC) surgery. However, its effect on cardiac fibroblasts (CFs), collagen secretion and the underlying mechanism remains unclear. In this study, DyLight® 488 green fluorescent dye or His-tagged proteins were used to label the exogenous serpinE2 protein. It was showed that extracellular serpinE2 translocated into CFs by low-density lipoprotein receptor-related protein 1 (LRP1) and urokinase plasminogen activator receptor (uPAR) of cell membrane through endocytosis. Knockdown of LRP1 or uPAR reduced the level of serpinE2 in CFs and down-regulated the collagen expression. Inhibition of the endocytosis of serpinE2 could inhibit ERK1/2 and β-catenin signaling pathways and subsequently attenuated collagen secretion. Knockdown of serpinE2 attenuates cardiac fibrosis in TAC mouse. We conclude that serpinE2 could be translocated into cardiac fibroblasts due to endocytosis through directly interact with the membrane protein LRP1 and uPAR, and this process activated the ERK1/2, β-catenin signaling pathways, consequently promoting collagen production.
研究领域:
心血管疾病
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细胞生物学
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分子生物学
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病理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CERAMICS INTERNATIONAL
·
2016
·Vol. 42
·期 14
·16248-16254
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IF 2.758
Limeng Liu,Guihong Geng,Wanxiu Hai,Yuhong Chen,Laner Wu
TaC ceramics were prepared by hot pressing at 1900 °C for 30 min under 30 MPa mechanical pressure. Effects of the addition of 20 mol% ZrC or 5–20 mol% ZrC plus 5 mol% Cu as sintering aids on densification , phase composition, microstructure and mechanical properties of the consolidated materials were investigated. The results showed the pure TaC had a relative density of 86%of the theoretical value. Densities >97% theoretical were reached in the materials when the sintering aids were used. ZrC and TaC reacted to form solid solutions . The grain average size decreased from 18 µm in the pure TaC to 6 µm at 20 mol% addition. Addition of 5 mol% Cu further refined the microstructures. The 20 mol% ZrC+5 mol% Cu and the 10 mol% ZrC+5 mol% Cu composition had good flexural strength of 608 MPa and fracture toughness of 5.4 MPa m 1/2 , respectively.
研究领域:
材料科学
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陶瓷
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机械工程
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DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Hong Chen,Junyan Liu,Mengna Wan,Shangyi Zhao,Xiaolong Li,Chuan Liu,Xuanping Zhao,Junjie Li,Qingzhen Ma,Cuixiang Li,Xueqin Wang
Fruit juice is kind of popular beverage food worldwidely, and its total antioxidant capacity (TAC) is generally considered as an important index for people’s rational diet, thus accurate evaluation of its TAC is essential for human health guidance. Herein, we constructed a sensitive Fe 3 O 4 @MnO 2 nanocatalyst that was delicately cored with magnetite nanoparticles (Fe 3 O 4 NPs) and encapsulated with manganese dioxide (MnO 2 ) nanoshells for fast and accurate TAC colorimetric analysis in fruit juice. The data showed the fabricated Fe 3 O 4 @MnO 2 nanocatalysts performed with remarkable oxidase mimetic activities and could catalyze oxidization of colorimetric substrate without additional H 2 O 2 , and the limit of detection (LOD) was about 0.39 μM for ascorbic acid (AA) evaluation. Applied in commercial fruit juice products, the fabricated Fe 3 O 4 @MnO 2 nanocatalysts showed a reliable and visual assessment for the accurate TAC analysis. In addition, the fabricated Fe 3 O 4 @MnO 2 nanocatalysts could be easily recycled due to their intrinsic superparamagnetic property. Together, an intellegent Fe 3 O 4 @MnO 2 nanocatalyst was well developed for visual TAC analysis in fruit juice with convenient applications in future.
研究领域:
纳米技术
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分析化学
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食品科学
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材料科学
✓ 已验证
产品关联
DOI
高影响因子
ACS Applied Nano Materials
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2024
·
IF 5.3
Peng Bo Niu,Yong Qiong Wang,Rong Hu,Tong Yang
With the improvement of the quality of life, the assessment of total antioxidant capacity (TAC) has attracted much attention in the field of a healthy diet, food analysis, or health guidelines. In this work, Cu nanoparticles (NPs)/CoO/carbon nanofibers (CNFs) as a promising nanozyme for peroxidase (POD) mimicking was synthesized by electrospinning and carbonation. Subsequently, a colorimetric sensing platform is constructed for TAC assessment by using 3,3′,5,5′-tetramethylbenzidine (TMB) as a chromogenic substrate based on Cu NPs/CoO/CNFs under presence of H2O2. The enhanced POD catalytic efficiency of Cu NPs/CoO/CNFs for TMB oxidation (ox-TMB) was about 3.2-fold higher than that of bare Cu NPs/CNFs or CoO/NFs, which was ascribed to the synergistic catalytic effect and the generation of heterogeneous interfaces between Cu NPs and CoO in CNFs. When some antioxidants such as AA, FA, TA, and GA were introduced in the chromogenic system, ox-TMB was reduced to colorless TMB, allowing the subsequent assessment of TAC. The results show that the limits of detection toward AA, FA, TA, and GA were 8.7, 8.3, 2.7, and 2.4 μM, respectively. Moreover, this proposed method was used for the TAC assay in real samples including fresh juices and commercial beverages, which showed good promise in practical applications such as food nutrition assessment, food analysis, personalized nutrition intervention, or staple food research.
研究领域:
材料科学
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分析化学
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食品科学
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生物技术
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产品关联
DOI
高影响因子
JOURNAL OF FOOD PROCESSING AND PRESERVATION
·
2020
·Vol. 45
·期 1
·e15087
·
IF 1.405
Sizhu Tian,Xuwen Li,Shuang Zang,Yongri Jin,Ziwei Zhang,Yong Yu
The phenolic compound contents, antioxidant capacities, and α-amylase and tyrosinase inhibitory activities of 10 wild and 19 cultivated blueberries grown in northeast China were evaluated. The results showed that the α-amylase inhibitory activities of the blueberries represented, by 1/IC 50 , correlated with their total phenolic contents (TPCs). Moreover, the tyrosinase inhibitory activities correlated with the total anthocyanin contents (TACs). High-performance liquid chromatography and electrospray mass spectrometry were conducted to determine and identify seven phenolic acids. The average caffeic acid contents of the cultivated and wild blueberries were 277.37 and 17.71 μg/g, respectively. The total phenolic, anthocyanin, and flavonoid contents were determined. Wild blueberries have higher TPCs, α-amylase inhibitory activities, and antioxidant capacities. Cultivated blueberries have higher TACs and exhibit tyrosinase inhibitory activities similar to those of the wild ones. Practical applications This study revealed that wild blueberries have high α-amylase inhibitory activities and are effective as natural α-amylase inhibitors for the treatment of diabetes. In addition, wild blueberries were found to be rich in phenolics and have high antioxidant capacities. Thus, because of these properties, wild blueberry products with antidiabetic and antioxidant potential can be developed for use in biomedical and pharmaceutical industries.
研究领域:
食品科学
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营养学
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药理学
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生物化学
✓ 已验证
产品关联
DOI
高影响因子
International Journal of Applied Ceramic Technology
·
2022
·Vol. 20
·期 3
·1487-1503
·
IF 2.328
Yao Li,Yu Wang,Jian Cui,Guo-Hua Zhang
High-entropy carbides (HECs) possess superior properties such as favorable corrosion resistance and marvelous hardness and are widely applied in specific extreme environments. Herein, a novel method was proposed for synthesizing HECs powders with raw materials of corresponding oxides and carbon black, which was composed of steps of ball-milling in stainless jar, carbothermic reduction at 1873 K, as well as the following calcium posttreatment at 1273 K to remove residual carbon by generating CaC 2 . Finally, pure HECs powders could be fabricated after acid leaching treatment. Under the current experimental conditions, besides HfC-TaC-ZrC-NbC quaternary system, a homogeneous solid solution could be fabricated by adding Mo or/and Ti, but it could not be achieved for systems with addition of V, W, or Cr, owing to the low lattice constant or different crystal structure of corresponding carbides. In the reduction step, iron resulted from ball milling process acted as the diffusion channel to promote the mutual diffusion of different components. Meanwhile, the calcium treatment temperature had a weak effect on particle size of HEC powders. HfC-TaC-ZrC-NbC-MoC-TiC powders prepared at the calcium posttreatment temperature of 1273 K had an oxygen content of .302% and an average grain size of 252 nm.
研究领域:
材料科学
·
冶金学
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
PHYTOMEDICINE
·
2024
·
IF 6.7
Peipei Cheng,Xinting Wang,Qian Liu,Tianshu Yang,Enrui Dai,Wanjing Sha,Huiyan Qu,Hua Zhou
ABSTRACT Background The terminal stage of all cardiovascular diseases typically culminates in heart failure (HF), with no effective intervention available to halt its progression. LuQi formula (LQF) has been employed in clinical for numerous years to significantly ameliorate cardiac function in HF patients. Nevertheless, the underlying mechanism of LQF's efficacy remains inadequately comprehended. Cardiomyocyte ferroptosis has served as a pathogenic mechanism in HF. The goal of the current experiment was to ascertain whether LQF ameliorates HF by preventing cardiomyocyte ferroptosis and to elucidate the intrinsic mechanism involved. Purpose This research objective is to investigate the impact and underlying mechanism of LQF attenuating cardiomyocyte ferroptosis in heart failure. Methods Transverse aortic constriction (TAC) was performed to construct the HF mouse model. Neonatal rat cardiomyocytes (NRCMs) were subjected to in vitro experiments. High-performance liquid chromatography (HPLC) identified the bioactive compounds in LQF. Transcriptomic and quantitative proteomic analyses revealed the potential targets of LQF anti-HF. Specifically, histological staining evaluated cardiac hypertrophy and fibrosis. Transmission electron microscopy (TEM) observed mitochondrial morphology. The content of Fe 2+ , ROS, MDA, GSH, and GSSH was detected using kits. Molecular docking evaluated the binding activities between essential active ingredients of LQF and critical proteins of cardiomyocyte ferroptosis. Mechanistically, the expression levels of Nrf2, Keap1, HO-1, SLC7A11, and GPX4 were evaluated using qPCR, Western blot (WB), or immunohistochemical staining. Results The primary nine active ingredients in LQF were detected. Transcriptomic and proteomic analyses demonstrated that LQF may ameliorate HF by preventing cardiomyocyte ferroptosis. Histomorphometric analyses revealed that LQF attenuates myocardial hypertrophy and fibrosis. TEM revealed that LQF diminished mitochondrial shrinkage and increased membrane density in myocardial tissue. Additionally, LQF diminished reactive oxygen species (ROS) generation in cardiomyocytes and suppressed cardiomyocyte ferroptosis. Furthermore, the molecular docking technique revealed that the primary active ingredients of LQF had suitable binding activities with Nrf2, GPX4, and SLC7A11. Western analysis further verified that LQF activated the Nrf2/GPX4 signaling axis. decreased SLC7A11 and HO-1 expression. Conclusions These results demonstrated that LQF prevents cardiomyocyte ferroptosis via activating Nrf2/GPX4 signaling axis and suppressing SLC7A11 and HO-1 expression. Concurrently, it contributed to elucidating the intrinsic mechanism of LQF and provided a scientific rationale for its development as a novel cardiovascular therapeutic drug.
研究领域:
心血管疾病
·
药理学
·
分子生物学
·
细胞生物学
·
信号转导
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）