LWT-FOOD SCIENCE AND TECHNOLOGY
·
2024
·
IF 6
Xiuhong Zhao,Yuxia Yang,Jinxi Cui,Yuxin Gan,Qingyu Yang
The effect of individually mixing Lactobacillus paracasei C5 and Lactobacillus pentosus D1 with yeast as starter cultures on bread flavor was investigated via electronic-nose, electronic-tongue, and GC × GC-ToF-MS methods The electronic-nose results showed that the bread smell could be completely distinguished. Electronic-tongue analysis revealed that the taste of mixed-fermented bread was positively correlated with sourness, sweetness, saltiness, and umami, while the taste of yeast-fermented bread was positively correlated with bitterness and astringency. GC × GC-TOF-MS was used to further detect volatile organic compounds, from which 15 ( L. paracasei bread) and 18 ( L. pentosus bread) major contributing aroma compounds were screened (ROAV ≥1 and VIP >1). Additionally, Lactobacillus was found to be positively correlated with 35 flavor substances and positively regulated the aroma profile of the bread, especially sweet-caramel, coffee-like, and roasted aromas. In conclusion, LAB involved in fermentation can improve the flavor of bread.
研究领域:
食品科学
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微生物学
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产品关联
DOI
高影响因子
CHEMBIOCHEM
·
2018
·Vol. 19
·期 11
·1154-1161
·
IF 2.774
Fuzhong Ouyang,Zhihao Zhao,Ruifang Gao,Rui Shi,Enhui Wu,Rui Lv,Guoqiang Xu,Jian Liu
Graphical Double distinction : A dual maleimide tagging method has been developed for both relative and absolute quantitation of cysteine-containing peptides (CCPs) in combination with MALDI-TOF mass spectrometry. The maleimides, N -methyl- and N -ethylmaleimide, with minimal differences in chemical structure, have been chosen to allow for the rapid tagging of CCPs with high efficiency. A dual maleimide (DuMal) tagging method has been developed for both relative and absolute quantitation of cysteine-containing peptides (CCPs) in combination with MALDI-TOF mass spectrometry. A pair of maleimides with minimal differences in their chemical structures, N -methylmaleimide and N ethylmaleimide, have been chosen to allow for the rapid (≈minutes) tagging of CCPs in the Michael addition reaction with high efficiency. It has been validated that the DuMal tagging technique is sensitive and reliable in the quantitative analysis of CCPs. Absolute quantitation of CCPs can be achieved with a detection limit as low as 7.3 n m . Relative quantitation of CCPs can be performed in various sample mixtures with consistent results (coefficient of variation <5 %). The DuMal tagging technique provides a sensitive and accurate approach for the quantitation of biomolecules containing thiol reactive sites; thus it is promising for protein detection, disease diagnosis, and biomarker discovery associated with post-translational modifications of cysteines.
研究领域:
生物化学
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蛋白质组学
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医学诊断
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产品关联
DOI
PubMed
高影响因子
Pathogens
·
2025
·Vol. 14
·期 5
·501
·
IF 3.3
Jia-Tong Han,Yu-Xuan Tang,Si-Yi Wu,Yi-Ran Chen,Zhan-Peng Zou,Hang Zeng,Zhongjia Yu
Escherichia colicontamination in poultry is a significant concern due to its potential to cause foodborne illness. The presence of extraintestinal pathogenicE. coli(ExPEC) strains in chicken carcasses can lead to severe human infections. This study investigates the prevalence, virulence, and antibiotic resistance ofE. coliisolates from chicken carcasses processed in both wet market and industrial environments, with a focus on the detection capabilities of MALDI-TOF MS. A total of 119E. coliisolates were obtained. Only a small proportion (5/119) carried enteropathogenic virulence genes. In contrast, 71.42% (85/119) of the isolates harbored multiple extraintestinal virulence genes. Among these,iucCandsitA, which are associated with systemic infections, were present in 68.24% (58/85) and 43.53% (37/85) of the isolates, respectively. Furthermore, 47.06% (56/119) of the isolates carrying at least two extraintestinal virulence genes were classified as ExPEC. Additionally, 94.6% (54/56) of ExPEC isolates were multidrug resistant (MDR), showing resistance to over three antibiotic classes, raising concerns about the spread of antibiotic resistance. MALDI-TOF MS profiling revealed significant heterogeneity among the ExPEC isolates, with no distinct clustering patterns based on processing environment or sampling site. These findings underscore the public health risks posed by ExPEC in poultry and emphasize the need for improved surveillance, stringent hygiene practices, and responsible antibiotic use in poultry production.
研究领域:
微生物学
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传染病学
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食品科学
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分子生物学
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DOI
PubMed
高影响因子
Phytochemistry Letters
·
2020
·
IF 1.459
Yin Zhang,Jianfeng Shi,Qianqian Fei,Yiping Huang,Fenxia Zhu,Jing Wang,Daoquan Tang,Bin Chen
The present study aimed to investigate the effects of steamed notoginseng single-flavored medicine, herb pairs, compound medicine on anemia rats induced by N-acetyl phenyl hydrazine (APH) to explore the underlying hematinic regulation mechanisms. Rats were randomly divided into seven groups: control group (CG); model group (MG); positive control group (PCG); steamed notoginseng group (SNG); ginseng group (GG); herbal pair group (HPG) and compound prescription group (CPG). Day 12 of the experiment, the blood routine examination of the rats was measured. At the same time, the rats' plasma and urine samples were collected. An Ultra-performance Liquid Chromatography-Quadrupole-Time-of-Flight / Mass Spectrometry (UPLC-Q-TOF/MS) with unsupervised principal component analysis (PCA) and supervised partial least squares discriminant analysis (PLS-DA) combined analytical method was established for metabolomics study of steamed notoginseng . Compared with the CG, red blood cell (RBC) and hemoglobin (Hb) levels in the MG were significantly lower (P < 0.01). The results of blood routine examination show that the model of the rats with blood deficiency syndrome have successfully modelled. Compared with the MG, steamed notoginseng can significantly increase the RBC level of rats (P < 0.01), indicating that steamed notoginseng has the blood tonic effect. The related indicators of each administration group showed a good trend of returning to normal. 44 potential biomarkers for hemolytic anemia were screened from plasma and urine samples. Compared with the CG, there were down-regulation seven biomarkers in plasma of the MG, such as retinol, l -valine and arachidonic acid, and 14 biomarkers up-regulated such as protoporphyrin IX and nicotinamide; 28 metabolites such as proline, histidine, phenylalanine, l -arginine and l -tyrosine were up-regulated in urine of the MG. The metabolic level of biomarkers could be changed to a normal state after the rats were given steamed notoginseng , herb pairs, and compound prescriptions. It is presumed that steamed notoginseng plays a role in blood enriching through 12 metabolic pathways, including valine, leucine and isoleucine biosynthesis, phenylalanine, tyrosine and tryptophan biosynthesis, retinol metabolism and so on.
研究领域:
药理学
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代谢组学
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中药学
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产品关联
DOI
高影响因子
ANALYST
·
2020
·Vol. 146
·期 1
·75-84
·
IF 3.978
Lei Li,Jiancheng Yu,Chengyi Xie,Chenlu Wang,Pengfei Guan,Jun Jack Hu,Keqi Tang
3-Pyridinylboronate, a zwitterionic boronic acid, displayed effective in situ ESI for reversible covalent tagging of saccharides in both cation and anion modes. The ion mobilities of thus-generated ions were examined with the Bruker timsTOF fleX instrument. Nine disaccharides were examined using this method. They have identical mass-to-charge ratios, differing only in monomer compositions, regio-linkages, and anomeric configurations (α or β). The IMS separations of the disaccharides from this method were compared with those from sodium adducts reported in the literature. The differentiation effects of this method on the disaccharide isomers were increased on average by an order of magnitude. Using this method, all the pairs of disaccharides selected from nine isomers were completely identified by comparing the mobility spectra of single-tagged and double-tagged ions.
研究领域:
分析化学
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质谱学
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碳水化合物化学
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产品关联
DOI
PubMed
高影响因子
CHROMATOGRAPHIA
·
2022
·Vol. 85
·期 12
·1017-1028
·
IF 2.213
Gao Yanlin,Chen Cen,Wang Ren,Li Zehua,Cheng Qiaoyuan
Currently, illegal addition of anti-infective drugs has been detected in some cosmetics to achieve rapid sterilization and anti-inflammatory effects. However, the abuse of anti-infective drugs may endanger the health of citizens. Thus, methods for the detection and quantitative determinations of such substances should be developed. In this paper, a method combining ultra-high-performance liquid chromatography with quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF HRMS) was designed. Its significance and potential were verified by the identification, confirmation, and quantification of 37 illegally added anti-infective drugs in cosmetics that claimed to have anti-acne effects. The method validation revealed a satisfactory linear relationship, and each coefficient of determination ( r 2 ) was higher than 0.9943. The LOD and LLOQ results were in the ranges of 0.0003–0.1063 μg g −1 and 0.0012–0.3545 μg g −1 , respectively. The accuracy ranged from 70.8 to 122.8%, whereas the intra- and inter-day precisions were in the ranges of 0.2–12.8% and 0.2–13.8%, correspondingly. The mean recoveries were in the range of 70.1–117.7%, and the matrix effects ranged from 73.0 to 121.4%. Of the 200 batches of cosmetics that claimed to have anti-acne effects, three batches were positive, with chemical components such as metronidazole, lincomycin, clindamycin, tinidazole and miconazole nitrate being discovered. Therefore, the Q-TOF spectrometry could be widely applied in routine screening of adulteration in cosmetics.
研究领域:
分析化学
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药学
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医学
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产品关联
DOI
高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
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2024
·
IF 3.1
HongXia Chen,Hao Zhou,Zhiwen Qi,Xingying Xue,ChengZhang Wang
Toxicodendron vernicifluum bark has been used for many years as a component in foods and as a traditional herbal medication. Unfortunately, the presence of urushiols, which induce allergies, limits its application. This study used a vortex-blending matrix solid-phase dispersion microextraction technique to extract urushiols from Toxicodendron vernicifluum bark. HPLC was used to evaluate the amounts of the extracted urushiols (15:0, 15:1, 15:2, and 15:3). The modified magnetic adsorbent was prepared through an in situ coprecipitation method and characterized using a variety of techniques. The optimized extraction conditions are as follows: using magnetic Zeolite Socony Mobil-Five as an adsorbent, a 1:2 sample/adsorbent ratio, 2.5 min of vortex-blending time, 4 mL of 0.1% (V/V) trifluoroacetic acid-methanol as the elution solvent and 8 min of ultrasound time. There was good linearity and high repeatability in the method. Furthermore, the limits of detection for the urushiols ranged from 0.20 to 0.50 μg/mL. Under the optimized conditions, 50 compounds were identified by ultra high performance liquid chromatography and quadrupole time-of-flight mass spectrometry. These compounds included 8 phenolic acids, 9 monomeric urushiols, 11 urushiol dimers, 10 other components, and 11 flavonoids. The suggested approach, which has the advantages of few stages and high extraction efficiency over existing extraction procedures, is a potentially useful method for obtaining and evaluating urushiols in raw materials or extracts.
研究领域:
植物化学
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分析化学
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药物化学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2016
·Vol. 17
·期 4
·412
·
IF 3.257
Ning Zhang,Haitao Chen,Baoguo Sun,Xueying Mao,Yuyu Zhang,Ying Zhou
To compare the volatile compounds of Chinese black truffle and white truffle from Yunnan province, this study presents the application of a direct solvent extraction/solvent-assisted flavor evaporation (DSE-SAFE) coupled with a comprehensive two-dimensional gas chromatography (GC × GC) high resolution time-of-flight mass spectrometry (HR-TOF/MS) and an electronic nose. Both of the analytical methods could distinguish the aroma profile of the two samples. In terms of the overall profile of truffle samples in this research, more kinds of acids were detected via the method of DSE-SAFE. Besides, compounds identified in black truffle (BT), but not in white truffle (WT), orvice versa, and those detected in both samples at different levels were considered to play an important role in differentiating the two samples. According to the analysis of electronic nose, the two samples could be separated, as well.Keywords:GC × GC/HR-TOF/MS;electronic nose;DSE-SAFE;Chinese truffles
研究领域:
分析化学
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食品科学
·
农学
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Lingping Hu,Yin Zhu,Hongwei Zhang,Xiaomei Zhang,Yujin Li,Qian Yao,Qiang Cai,Yaqin Hu
Food fraud is common in the tuna industry because of the economic benefits involved. Ensuring the authenticity of tuna species is crucial for protecting both consumers and tuna stocks. In this study, GC-Q-TOF and UPLC-Q/Orbitrap mass spectrometry-based metabolomics were used to investigate the metabolite profiles of three commercial tuna species (skipjack tuna, bigeye tuna and yellowfin tuna). A total of 22 and 77 metabolites were identified with high confidence using GC-Q-TOF and UPLC-Q/Orbitrap mass spectrometry , respectively. Further screening via chemometrics revealed that 38 metabolites could potentially serve as potential biomarkers. Hierarchical cluster analysis showed that the screened metabolite biomarkers successfully distinguished the three tested tuna species. Furthermore, a total of 27 metabolic pathways were identified through enrichment analysis based on the Kyoto Encyclopaedia of Genes and Genomes (KEGG) pathways.
研究领域:
食品科学
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代谢组学
·
生物信息学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2024
·
IF 4
Shuai Zhuang,Huihui Ming,Wenjie Yu,Liping Luo
Royal jelly (RJ) is a highly perishable food during storage. However, the quality of RJ is hard to evaluate due to its complex and variable compositions. Nanoliter electrospray ionization mass spectrometry (nano-ESI-MS) is an emerging direct mass spectrometry method suitable for rapid analysis of complex food components and food quality. In this study, we investigated the freshness and metabolite changes of RJ stored at 4 °C and 25 °C using nano-ESI-MS and verified the results with UPLC-Q/TOF-MS. The nano-ESI-MS identified 20 compounds and quantified 9 metabolites with high stability and reproducibility. Multivariate analysis of the nano-ESI-MS data distinguished RJ of different freshness and suggested 45–60 and 7 days as the freshness threshold of RJ stored at 4 °C and 25 °C, respectively. The multivariate analysis of UPLC-Q/TOF-MS data output similar results to that of nano-ESI-MS. The relative abundance changes of metabolites identified by UPLC-Q/TOF-MS were approximately consistent with the quantification results of nano-ESI-MS, indicating the reliability of nano-ESI-MS in monitoring RJ freshness. This study proved the potential of nano-ESI-MS as a rapid method for monitoring RJ freshness and thus promoted the quality control of RJ during production.
研究领域:
食品科学
·
分析化学
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产品关联
DOI
高影响因子
ACS Omega
·
2020
·
IF 2.87
Yumeng Yang,Dan Gao,Rui Qian,Yuyang Jiang
Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) using conventional organic matrices for detection of small molecules has some limitations, such as heterogeneous analyte/matrix co-crystals, as well as interference of matrices in the low-molecular-weight range. In this work, a zeolite framework nanomaterial, TS-1, was applied as a MALDI matrix for the analysis of small molecules by MALDI-MS for the first time. To improve the signal intensity and reproducibility, TS-1 was modified with polydopamine (TS-1@PDA). Using TS-1@PDA as a matrix, organic substances in the low-molecular-weight region such as amino acids, nucleosides, peptides, oligosaccharides, and fatty acids can be detected by MALDI-MS in positive ion mode. Compared with traditional organic matrices like 2,5-dihydroxybenzoic acid (2,5-DHB) and α-cyano-4-hydroxycinnamic acid (CHCA), TS-1@PDA has the advantages including the formation of uniform sample spots, small background interference at low molecular weight, and better salt tolerance. Furthermore, this matrix was employed for the analysis of endogenous glucose in urine samples, and the level of glucose was quantified with a linear range of 0–10 mM (R2 > 0.98). The results demonstrated that TS-1@PDA has the potential to be used as an effective MALDI matrix for the analysis of small molecules in biological samples with excellent reproducibility and moderate sensitivity.
研究领域:
分析化学
·
生物医学
·
质谱技术
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产品关联
DOI
PubMed
高影响因子
ANALYST
·
2025
·
IF 3.3
Zhirou Zhang,Enyun Xing,Wenzhuo Zhao,Minghui Song,Cuiping Zhang,Hong Liu,Xiaomin Li,Hongxiu Yu
Reducing the time required for the detection of bacteria in blood samples is a critical area of investigation in the field of clinical diagnosis. Positive blood culture samples often require a plate culture stage due to the interference of blood cells and proteins, which can result in significant delays before the isolation of single colonies suitable for matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis. In this study, we developed a non-specific enrichment strategy based on SiO2-encapsulated Fe3O4 nanoparticles combined with MALDI-TOF MS for direct identification of bacteria from aqueous environments or positive blood culture samples. Three distinct types of Fe3O4@SiO2 magnetic nanoparticles (MNPs) with unique surface morphologies were developed: spherical MNPs with smooth surfaces (Fe3O4@SN), mesoporous silica coated MNPs (Fe3O4@MSN), and MNPs exhibiting a viral spiked structure (Fe3O4@VSN). These MNPs exhibited excellent binding affinity towards both Staphylococcus aureus and Klebsiella pneumoniae in PBS and artificial saliva solutions. Furthermore, the strategy of using Fe3O4@VSN, which involves non-specific interactions between bacterial cells and the virus-like surface, resulted in a dramatic reduction in the minimum detectable concentrations of target pathogens by up to 1000-fold compared to conventional methods. Our results demonstrate that the use of Fe3O4@VSN has the potential to significantly reduce the processing time required after blood culture and may be useful for enrichment and identification of microorganisms in complex clinical samples.
研究领域:
医学诊断
·
微生物学
·
纳米技术
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）