Journal of Pharmaceutical Innovation
·
2019
·Vol. 15
·期 1
·155-162
·
IF 1.452
Huang Zhengwei,Wu Mingjun,Ma Cheng,Bai Xuequn,Zhang Xuejuan,Zhao Ziyu,Huang Ying,Pan Xin,Wu Chuanbin
Objective Solid lipid nanoparticles (SLN) mainly consist of lipids and surfactants. However, the toxicity of surfactant cannot be ignored, as the surfactant may cause unexpected side effect. This study established model SLN with different surfactants, Tween 80 (T80) and poloxamer 188 (P188). It was aimed to establish ultraviolet-visible (UV-Vis) spectroscopy methods for quantifying the contents of T80 and P188 in the model SLN. Methods The model SLN were produced by emulsification-precipitation method, and their particle size distribution was analyzed. The calibration curves for the contents of T80 and P188 were established. Surfactants in the model SLN were extracted by ultrasonication, and UV-Vis spectroscopy methods were utilized for quantification of surfactants in SLN. Results The deviation of precision and accuracy in P188 and T80 assay were acceptable, which indicated the method could be employed in sample quantification. Ten minutes was considered as the optimal ultrasonication time. And the contents of P188 and T80 were successfully measured by the established UV-Vis methods. Conclusion The UV-Vis spectroscopy methods were developed for the quantification of T80 and P188 in the model SLN. It is expected that the methods could be extended to more SLN systems and applied for the quantification of other surfactants.
研究领域:
药剂学
·
分析化学
✓ 已验证
DOI
高影响因子
BIOTECHNOLOGY AND BIOENGINEERING
·
2016
·Vol. 114
·期 3
·543-551
·
IF 4.243
Renliang Huang,Hong Guo,Rongxin Su,Wei Qi,Zhimin He
Recycling cellulases by substrate adsorption is a promising strategy for reducing the enzyme cost of cellulosic ethanol production. However, β-glucosidase has no carbohydrate-binding module (CBM). Thus, additional enzymes are required in each cycle to achieve a high ethanol yield. In this study, we report a new method of recycling cellulases without β-glucosidase supplementation using lignocellulosic substrate, an engineered strain expressing β-glucosidase and Tween 80. The cellulases and Tween 80 were added to an aqueous suspension of diluted sulfuric acid/ammonia-treated corncobs in a simultaneous saccharification and fermentation (SSF) process for ethanol production. Subsequently, the addition of fresh pretreated corncobs to the fermentation liquor and remaining solid residue provided substrates with absorbed cellulases for the next SSF cycle. This method provided excellent ethanol production in three successive SSF cycles without requiring the addition of new cellulases. For a 10% (w/v) solid loading, a cellulase dosage of 30 filter paper units (FPU)/g cellulose, 0.5% Tween 80, and 2 g/L of the engineered strain, approximately 90% of the initial ethanol concentration from the first SSF process was obtained in the next two SSF processes, with a total ethanol production of 306.27 g/kg corncobs and an enzyme productivity of 0.044 g/FPU. Tween 80 played an important role in enhancing cellulase recovery. This new enzyme recycling method is more efficient and practical than other reported methods. Biotechnol. Bioeng. 2017;114: 543–551. © 2016 Wiley Periodicals, Inc.
研究领域:
生物技术
·
酶工程
·
发酵工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Nano Materials
·
2024
·
IF 5.3
Ruijuan Wang,Chunliu Ma,Haitao Yan,Hailiang Zhao,Pu Wang,Shoubing Zhang,Jianwei Ju,Shuyan Yu,Zhigang Yin
Co-encapsulated hydrophilic vitamin C (Vc) and hydrophobic xanthoxylin (GX-50) liposomes (L-Vc-GX-50) were prepared using ethanol injection and ultrasonic methods and characterized by dynamical light scattering, ζ-potential, cryogenic transmission electron microscopy, UV–vis spectroscopy, Fourier transform infrared spectroscopy, antioxidant capacity, tyrosinase activity, and in vitro permeation measurements. L-Vc-GX-50 are formed by the assembly of egg yolk lecithin, cholesterol, and tween 80, with Vc and GX-50 encapsulated in their hydrophilic and hydrophobic cavities, respectively. By considering the hydrodynamic radius (Rh), ζ-potential, and encapsulation efficiency (EE) of L-Vc-GX-50, the optimum formulation was determined to be egg yolk lecithin/cholesterol/tween 80/GX-50 or Vc (15:3:3:1, w/w) with phosphate buffer (pH 6.8, 0.01 M). The as-prepared L-Vc-GX-50 have an average Rh of 114 nm, a negative ζ-potential (−23.63 mV), a high EE of Vc (73.3%) and GX-50 (87.4%), and a unilamellar vesicle structure. Compared co-encapsulated L-Vc-GX-50 with single encapsulated Vc or GX-50 liposomes (L-Vc or L-GX-50), the EE of Vc in L-Vc-GX-50 is obviously higher than that of L-Vc, while the EE of GX-50 in L-Vc-GX-50 is similar to that in L-GX-50; the antioxidant capacity and tyrosinase inhibition activity of L-Vc-GX-50 are higher than those of L-GX-50 or L-Vc. The in vitro permeation data indicate that the final cumulative permeation amount of Vc and GX-50 in L-Vc-GX-50 can reach around 2.1 and 1.3 mg/cm2, respectively. The release kinetics fitting data show that the release kinetics of L-Vc-GX-50 follows the Korsmeyer–Peppas model and that the release of active ingredients is mainly dominated by the synergistic effect of diffusion release and rupture release. Moreover, L-Vc-GX-50 has good low-temperature storage stability, and the storage stability of GX-50 in L-Vc-GX-50 is significantly improved compared to L-GX-50. The main objective of this study is to improve the instability of Vc and the insolubility of GX-50 in cosmetics.
研究领域:
药剂学
·
纳米技术
·
化妆品
·
药物递送系统
·
分析化学
·
生物物理学
✓ 已验证
产品关联
DOI
高影响因子
Food Bioscience
·
2023
·
IF 5.2
Tao Yang,Chang Liu,Yan Zheng,Tristan C. Liu,Kexin Li,Jianguo Liu,Yaowei Liu,Peng Zhou
Conversion of major ginsenosides into minor ginsenosides improves its pharmacological effects. This study managed to convert major ginsenosides into minor ginsenoside Rg3 and compound K (CK) by optimizing acidolysis conditions (pH, incubation temperatures and time) and evaluated the effects of the whey protein isolate (WPI)/Tween 80 ratio on the physicochemical properties of ginsenoside nanoemulsions . The optimal conditions for acidolysis are incubation at 110 °C for 3 h at a pH of 2.0, whereafter the yield of ginsenoside Rg3 and CK was 2.16 mg/g and 2.80 mg/g, respectively. For the ginsenoside nanoemulsions with 0.5% WPI as emulsifier, as Tween 80 increased from 0.1% to 0.5%, the mean droplet diameter gradually decreased from ∼740 nm to ∼310 nm. Accordingly, the encapsulation efficiency of Rg3 and CK increased by 8.36% and 9.03%, respectively, and the centrifugal stability constant increased by 49.82%. These results showed that nanoemulsion prepared with 0.5% WPI and 0.5% Tween 80 exhibited higher encapsulation efficiency and physicochemical stability. In addition, ginsenoside nanoemulsion prepared with 0.5% WPI and 0.5% Tween 80 showed great industrial applicability during freeze-thaw cycles, heat treatments and storage. Generally, the incorporation of Tween 80 as a co-emulsifier improved the encapsulation efficiency and physicochemical stability of WPI-stabilized ginsenoside nanoemulsions, which may improve its bioaccessibility, by stabilizing ginsenoside emulsions during further processing, such as during thermal pasteurization and microencapsulation .
研究领域:
食品科学
·
药学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2024
·
IF 9.4
Sijun Huang,Liqiang Yu,Kun Zhang,Jiachen Lv,Henglong Xiang,Dongwei Zhu,Huan Li,Shuning Zhang,Xinyu Liu,Ying Wang,Yi Guo,Li Xu
The spontaneous adsorption of proteins onto nanoparticles, known as the protein corona, provides a unique perspective for designing protein-sensing biosensors. This study proposes a tailored protein corona method mediated by Tween-20 and develops a reverse-capture approach for protein quantification assays. The protein-coated microplate captures titanium dioxide nanosheets (TiO 2 -NS) in a phosphate buffer containing Tween-20 and generates fluorescence signals via the photocatalytic reduction of resazurin to resorufin, thereby indicating the amount of protein. The linear range of the current assay was 0.5–5 ng/mL, and the limit of detection (LOD) was 0.28 ng/mL, which is 100–1000 times more sensitive than the classical colorimetric methods. This method is suitable for the determination of proteins in artificial urine and has broad potential for practical applications.
研究领域:
生物化学
·
分析化学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2010
·
IF 1.988
Guolin Song,Sude Ma,Guoyi Tang,Xiaowei Wang
In this paper, with the aid of ultrasonic irradiation, hydrophobic magnesium hydroxide (MH) nanoparticles were synthesized via one-step precipitation reaction, in which the reactant solutions were simultaneously added into an organic phase instead of an aqueous phase. Tween 80 was added as a stabilizer , dispersant and surface modifier. During this synthesis process, ultrasonic irradiation could restrict the crystal growth of MH on each lattice plane and improve hydrophobicity of MH nanoparticles. Addition of Tween 80 was favourable to obtain smaller sized MH nanoparticles with even crystal growth and better dispersibility, and to further enhance the hydrophobic property of MH nanoparticles based on its bridging function between surface of MH nanoparticles and aliphatic compounds in organic phase. In addition, the morphology and the properties of obtained MH nanoparticles were characterized by XRD , FESEM , FT-IR, TGA , respectively. The improved hydrophobicity and dispersibility of surface modified MH nanoparticles were also verified by the sedimentation tests.
研究领域:
材料科学
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
Food and Bioprocess Technology
·
2014
·Vol. 7
·期 11
·3121-3135
·
IF 3.126
Tian Ye,Fu Nan,Wu Winston Duo,Zhu Danhua,Huang Jinjian,Yun Shuo,Chen Xiao Dong
Dairy industries often spray lecithin, a food-grade surfactant, to spray-dried whole milk powders while fluidisation to produce instant powders. Though adding surfactant to milk feed was often reported to improve the wettability of dried powders, this approach was not favourably used. The present study investigated the effects of surfactant addition into high solids milk feed before drying on the wettability of whole milk particles. Adding either 0.1 wt.% Tween 80 or 0.1 wt.% lecithin to un-concentrated whole milk led to a significant wettability improvement of spray-dried powders. At higher feed solids levels of 23 and 33 wt.%, the wetting process of pure milk powders was comparatively rapid, but the surfactant-added powders showed similar wettability to the pure milk powders. The development of powder wettability as drying progressed was investigated using single droplet-drying technique for 32 and 43 wt.% whole milk in the presence and absence with surfactants. The technique captured an advanced shell formation during drying of higher solids milk. The wettability of surface shells formed by surfactant-added milk was similar to those formed by pure milk throughout drying, from initial shell formation to final drying stage. By contrast, coating surfactants on the outer layer of particles being dried could substantially improve wettability. The rapid shell formation and the slow material diffusion owing to the high medium viscosity were considered the main factors limiting the migration of surfactant molecules towards droplet surface during drying.
研究领域:
食品科学
·
乳品加工
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2020
·
IF 5.162
Xiaoxiao Sun,Chuqiao Pan,Zhuoyang Ying,Dongyan Yu,Xitai Duan,Fangfang Huang,Junhong Ling,Xiao-kun Ouyang
We aimed to develop a stable nanosystem with k -carrageenan (Kc), an anionic polysaccharide , combined with tween 80 to stabilize zein nanoparticles. The experimental results showed that the particle size, zeta-potential, and stability of the system depend on the Kc amount when the zein amount was unchanged. We found that the nanosystem with 5.0 and 10.0 mg Kc had a smaller particle size (250.1 ± 0.9 and 287.6 ± 1.4 nm, respectively), low polydispersity index (PDI, ≤ 0.20), and high stability when the external conditions changed. No obvious flocculation or deposition was observed at pH of 3–9, salt ion concentrations of 0–500 mmol/L, temperature of 30 °C and 50 °C–90 °C, and storage at 4 °C for 30 days. When the Kc-ZNP system was loaded with curcumin (Cur), there was a good encapsulation effect (5.0 mg Kc with 1.00 mg Cur (EE (%) = 34.69% ± 2.02%, size = 380.23 ± 5.80 nm, PDI = 0.252 ± 0.018) and 10.0 mg Kc with 2.00 mg Cur (EE (%) = 20.13% ± 1.05%, size = 431.27 ± 0.78 nm, PDI = 0.269 ± 0.020), ensuring that the particle size was not too large. Therefore, Kc–ZNPs could be a potential delivery system for fat-soluble drugs.
研究领域:
食品科学
·
药物递送系统
·
生物材料
✓ 已验证
DOI
高影响因子
RSC Advances
·
2020
·Vol. 10
·期 44
·26059-26066
·
IF 3.119
Shuwei Liu,Lu Wang,Bin Zhao,Ze Wang,Yinyu Wang,Bin Sun,Yi Liu
In clinical tumor therapy, traditional treatments such as surgery, radiotherapy and chemotherapy all have their own limitations. With the development of nanotechnology, new therapeutic methods based on nanomaterials such as photothermal therapy (PTT) have also emerged. PTT takes advantage of the poor thermal tolerance of tumor cells and uses the heat generated by photothermal reagents to kill tumor cells. A transition metal sulfide represented as Cu2S is an ideal photothermal reagent because of its easy preparation, high extinction coefficient and photothermal conversion efficiency. Surface modification of nanoparticles (NPs) is also necessary, which not only can reduce toxicity and improve colloidal stability, but also can provide the possibility of further chemotherapeutic drug loading. In this work, we report the fabrication of Tween-20 (Tw20)-modified and doxorubicin (Dox)-loaded Cu2S NPs (Cu2S/Dox@Tw20 NPs), which significantly improves the performance in tumor therapy. Apart from the enhancement of colloidal stability and biocompatibility, the drug loading rate of Dox in Tw20 reaches 11.3%. Because of the loading of Dox, Cu2S/Dox@Tw20 NPs exhibit chemotherapeutic behaviors and the tumor inhibition rate is 76.2%. Further combined with a near-infrared laser, the high temperature directly leads to the apoptosis of a large number of tumor cells, while the release of chemotherapeutic drugs under heat can not only continue to kill residual tumor cells, but also inhibit tumor recurrence. Therefore, with the combination of PTT and chemotherapy, the tumor was completely eliminated. Both hematological analysis and histopathological analysis proved that our experiments are safe.
研究领域:
纳米技术
·
肿瘤学
·
药学
·
生物医学工程
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF FOOD SCIENCE
·
2022
·Vol. 87
·期 9
·4027-4039
·
IF 3.693
Miral Javed,Tarun Belwal,Hao Huang,Yanqun Xu,Fatima-ezzahra Ettoumi,Li Li,Xuezhi Fang,Zisheng Luo
Camellia oleifera shells are abundant in polyphenolic compounds. Green extraction methods of polyphenolic compounds are essential to ensure product quality, efficiency, process cost, environment, and safety. This study investigated the effect of Tween 80 and Rhamnolipid surfactants on the production and utilization of stabilized carbon dioxide nanobubbles (CO 2 -NBs). The results confirmed the presence of the CO 2 -NBs in ultra-pure water with a concentration of 8.45 ± 1.05 × 10 8 ml –1 , among which the stable CO 2 -NBs possessed a mean size of 40–90 nm and a negative zeta potential (−41.6 ± 1.3 mV). Further, the efficiency of CO 2 -NBs combined with ultrasonication (CO 2 -NBs-Rh-UAE) was evaluated to extract polyphenols from Camellia oleifera shells (waste). The CO 2 -NBs treatment with ultrasonication showed the highest total phenolic content (TPC) and total flavonoid content (TFC) (36.75 ± 0.22 mg GAE/g DW and 24.06 ± 0.22 mg RE/g DW, respectively). Overall, this study demonstrated an innovative approach for producing, stabilizing, and utilizing biosurfactant stabilized CO 2 -NBs to extract polyphenolic compounds from the waste agricultural products. These findings highlighted the potential application of biosurfactant-stabilized CO 2 -NBs.
研究领域:
食品科学
·
环境科学
✓ 已验证
DOI
PubMed
高影响因子
BIOCHEMICAL ENGINEERING JOURNAL
·
2020
·
IF 3.475
Conghai Li,Juan Zhao,Zhijin Zhang,Yanjun Jiang,Muhammad Bilal,Yunhong Jiang,Shiru Jia,Jiandong Cui
Lipase-inorganic hybrid nanoflowers were prepared using Ca 3 (PO 4 ) 2 as the inorganic component and lipase from Aspergillus oryzae ( A. oryzae ) as the organic component. The influences of metal ions with different valence, various additives (surfactant), and synthesis conditions on the activity of the lipase hybrid nanoflowers were systematically investigated. Results revealed that the valence state of metal ions played an important role on the shape and activity of lipase hybrid nanoflowers. The synthesized lipase hybrid nanoflowers using bivalence metal ions (Ca 2+ , Mn 2+ , and Zn 2+ ) as the inorganic components exhibited relative high activity. However, very low activities were observed in the lipase hybrid nanoflowers using univalent metal ions (Ag + ) or trivalent metal ions (Al 3+ , Fe 3+ ). More importantly, Ca 2+ not only induced self-assemble of lipase hybrid nanoflowers, but also activated the enzyme activity by inducing conformational changes in lipase from A. oryzae . As a result, lipase/Ca 3 (PO 4 ) 2 hybrid nanoflowers (hNF-lipase) exhibited the high activity. The hNF-lipase displayed 9, 12, and 61 folds higher activity than lipase/Ag 3 PO 4 hybrid nanoflowers, lipase/AlPO 4 hybrid nanoflowers, and lipase/FePO 4 nanoflowers, respectively. Compared with free lipase, the hNF-lipase displayed 172 % increase in activities by using 0.15 mM Tween-80 as an activity inducer (activated hNF-lipase). Furthermore, the hNF-lipase and activated hNF-lipase exhibited increased stability against high temperature and denaturant, and had good storage stability and reusability.
研究领域:
生物化学
·
纳米技术
·
酶学
✓ 已验证
产品关联
DOI
高影响因子
CARBOHYDRATE POLYMERS
·
2026
·
IF 13.2
Ning Li,Juan Huang,Chen Chen,Haiyan Yu,Huaixiang Tian
In the field of food colloid systems, elucidating the synergistic stabilization mechanism of composite emulsifiers at the interface in Pickering emulsions is crucial for designing high-performance emulsions. This study investigates the synergistic stabilization mechanism of β-cyclodextrin (β-CD) and Tween 60 in Pickering emulsions using experiments and dissipative particle dynamics (DPD) simulations. The optimal mixing ratio (β-CD: Tween 60 = 1:3) achieves the lowest oil–water interfacial tension (5.38 ± 0.06 mN/m) and the smallest rearrangement rate constant (K r ). All systems exhibit contact angles below 90°, forming oil-in-water emulsions. DPD simulations reveal that at the 1:3 ratio, β-CD and Tween 60 form a uniform, dense interfacial film on oil droplets. Radial distribution function analysis indicates strong short-range interactions between the two emulsifiers. The relative concentration profiles show that at this ratio, the peaks of the β-CD and Tween 60 curves are highly consistent, and the interfacial arrangement is the most ordered, confirming the mechanism of dense film formation through synergistic adsorption. Excess β-CD promotes self-aggregation, disrupting the synergistic structure. This work provides a theoretical foundation for designing high-performance Pickering emulsions.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）