JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2024
·
IF 4
Huanhuan Li,Qiyi Bei,Md. Mehedi Hassan,Murugavelu Marimuthu,Selorm Yao-Say Solomon Adade,Quansheng Chen,Muhammad Zareef
Abuse of tetracycline (TC) leads to the development of drug-resistant bacterial strains that threaten public health. This paper proposed a novel Cu 2+ -modulated UCNPs@RBD sensor for detecting TC. The sensor utilizes rhodamine B derivatives (RBD) that bind to Cu 2+ , forming RBD-Cu through a spirolactam open-loop reaction. The fluorescence of upconversion nanoparticles (UCNPs) is quenched by RBD-Cu via the inner filter effect (IFE). Upon addition of TC, TC-Cu forms due to electron-donor groups in TC, which decreases the generation of RBD-Cu, thereby enhancing the fluorescence of UCNPs . Under optimized conditions, the sensor exhibits remarkable accuracy across a concentration range of 50–50000 µg L −1 , with a limit of detection (LOD) of 1.72 µg L −1 . Moreover, the sensor demonstrates high selectivity for TC, validated by its application in food samples. The relative standard deviation (RSD) in real sample analysis was below 4.17 %, and the results were consistent with the HPLC method ( P > 0.05). Hence, the sensor exhibited a high level of sensitivity and reliability, making it suitable for the detection of TC in food.
研究领域:
食品科学
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分析化学
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生物传感
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产品关联
DOI
高影响因子
CHINESE JOURNAL OF CATALYSIS
·
2018
·
IF 3.525
Mengli Zhao,Wanni Wang,Chenxi Huang,Wang Dong,Yang Wang,Sheng Cheng,Huiqing Wang,Haisheng Qian
Photocatalysis driven by near-infrared (NIR) light is of scientific and technological interest for exploiting solar energy. In this study, we demonstrate a facile hydrothermal process to synthesize core-shell nanoparticles combining upconversion nanoparticles (UCNPs) and alloyed Zn x Cd 1- x S, which can be excited using NIR or visible light. Morphologies, phase, and chemical composition have been investigated using field-emission scanning electron microscopy, transmission electron microscopy, X-ray diffraction analysis, and atomic absorption spectroscopy. Moreover, we found that amorphous TiO 2 layers existing in the final samples play an important role in formation of UCNPs@Zn x Cd 1- x S yolk-shell nanoparticles, which bind the as-prepared Zn x Cd 1- x S nanoparticles tightly to form yolk-shell nanoparticles. The chemical composition of alloyed Zn x Cd 1- x S can be tunable by adjusting the amount of the Cd and Zn source compounds. The photochemical reduction of Cr(VI) in water has been performed to study the photocatalytic performance under irradiation by NIR light or a simulated solar light, showing efficient photoreduction and Cr(VI) removal over the as-prepared UCNPs@Zn x Cd 1- x S/TiO 2 yolk-shell nanoparticles. The as-prepared UCNPs@Zn x Cd 1- x S/TiO 2 nanoparticles show excellent production of hydroxyl radicals, which are responsible for the photochemical reduction of Cr(VI) to Cr(III). This study will provide an alternative strategy for environmental wastewater treatment , making full use of solar energy.
研究领域:
环境科学
·
材料科学
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产品关联
DOI
高影响因子
PHYSICA B-CONDENSED MATTER
·
2021
·
IF 2.436
Yang Yu,Sai Xu,Muhan Jiang,Ruixin Song,Jie Li,Yuefeng Gao,Xizhen Zhang,Xiangping Li,Hongquan Yu,Baojiu Chen
Upconversion core-shell nanoparticles with dual-function of temperature sensing and photothermal conversion are preferable in diagnose and treatment of cancer. Herein, NaYF 4 :Tm 3+ /Yb 3+ @NaYF 4 :Sm 3+ /Yb 3+ upconversion nanoparticles have been synthesized by a high-temperature thermal decomposition method. XRD and TEM are used to characterize the crystal structure and morphology of the samples. Temperature dependent upconversion spectra are measured under excitation of 980 nm within the scope of 303–498 K. The investigation on temperature sensing properties are on the basis of the luminescence intensity ratio of Tm 3+ . The designed core shell nanoparticles and bare core nanoparticles both have good temperature sensing performances, and the sensing properties of both are similar. Furthermore, the photothermal conversion performances are investigated ground on the power dependent temperature measurement, ethanol evaporation experiment and visual infrared thermal images. It is found that the photothermal conversion ability of the core-shell UCNPs is greatly improved by coating of the NaYF 4 : Sm 3+ /Yb 3+ outer shell.
研究领域:
纳米技术
·
生物医学工程
·
材料科学
·
癌症研究
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产品关联
DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2024
·
IF 4.3
Zi-Hang Zhang,Xiao-Bo Zhang,Pu Wang,Su-Hang Xu,Zuo-Qin Liang,Chang-Qing Ye,Xiao-Mei Wang
Iron ion (Fe 3+ ) detection is crucial for human health since it plays a crucial role in many physiological activities. In this work, a novel Schiff-base functionalized cyanine derivative (CyPy) was synthesized, which was successfully assembled on the surface of upconversion nanoparticles (UCNPs) through an amphiphilic polymer encapsulation method. In the as-designed nanoprobe, CyPy, a recognizer of Fe 3+ , is served as energy donor and β-NaYF 4 :Yb,Er upconversion nanoparticles are adopted as energy acceptor. As a result, a 93-fold enhancement of upconversion luminescence is achieved. The efficient energy transfer from CyPy to β-NaYF 4 :Yb,Er endows the nanoprobe a high sensitivity for Fe 3+ in water with a low detection limit of 0.21 μM. Moreover, the nanoprobe has been successfully applied for Fe 3+ determination in human serum and tap water samples with recovery ranges of 95 %-105 % and 97 %-106 %, respectively. Moreover, their relative standard deviations are all below 3.72 %. This work provides a sensitive and efficient methodology for Fe 3+ detection in clinical and environmental testing.
研究领域:
分析化学
·
材料科学
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环境科学
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生物医学工程
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DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2022
·
IF 16.744
Yi Zhang,Da Luo,Yin Zhang,Qi-Hai Zhang,Qing-Yuan Ji,Shi-Kang Zhou,Shan Huang,Li-li Li,Feng Lu,Wei-Feng Yao,Fang-Fang Cheng,Jun-Jie Zhu,Li Zhang
Upconversion nanoparticles (UCNPs) are promising materials for imaging biomolecules and predicting the progression of diseases, especially for tumors. However, conventional luminescence resonance energy transfer (LRET) based nanosensors remain challenging with complicated modification and unsatisfactory sensitivity. Herein, a sensing platform integrating confined emission effect and cycling amplification strategy into one substrate with high LRET efficiency and sensitivity has been developed for the in - vitro and in - vivo detection of microRNA-21. Washing UCNPs with hydrochloric acid was conducted for electrostatic absorption of 8–17 DNAzyme labeled with BHQ1 (8–17 DNAzyme-BHQ1) and complementary capturing template-aDNA3 complex (CPT-aDNA3) to form energy donor–acceptor pair (UCNPs-BHQ1) and capture miR-21. It not only avoids the complex modification procedure but also shortens the distance between the donor (UCNPs) and the acceptor (BHQ1). The quenching efficiency of 60.94 % was obtained. Targeted miR-21 could bind with CPT to release aDNA3 that can unlock 8–17 DNAzyme-BHQ1 to form aDNA3-DNAzyme-BHQ1 duplex. This aDNA3-DNAzyme-BHQ1 duplex owning -rA- group can be cleaved by Zn 2+ so that UCNPs restored green UCL emission because of the desorption of BHQ1. aDNA3 becomes dissociative again to trigger the next cycle. This [email protected] /CPT- aDNA3 achieved a wide linear range from 4 × 10 -14 M to 1 × 10 -8 M with a low limit detection of 31 fM for the sensitive detection of miR-21 in vitro . Furthermore, it was successfully applied to the real-time intracellular detection of miR-21 in HeLa cells and in - vivo monitoring of miR-21 expression in KS-01 treated tumor-bearing mouse. This proposed DNA-conjugated upconversion nanoamplicon provides new sights into monitoring of anti-cancer active ingredients efficacy and the progress evaluation of miRNA-related diseases.
研究领域:
纳米技术
·
生物医学工程
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分子生物学
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癌症研究
·
生物成像
✓ 已验证
产品关联
DOI
高影响因子
Journal of Physical Chemistry C
·
2023
·
IF 3.7
Song Jia,Ming Liu,Xinhu Liang,Xiaojuan Li,Xuefei Wang,Zhiyuan Tian
Lanthanide-doped upconversion nanoparticles (Ln-UCNPs) hold great potential in anticounterfeiting applications owing to their salient optical properties. However, a single-mode anticounterfeiting strategy based on common Ln-UCNPs typically confronts challenges in terms of imitation based on alternatives with a similar luminescence feature. Multilevel anticounterfeiting strategies on the basis of the multicolor beaconing luminescence generated upon dual- or multimode excitation are promising for addressing such a challenge and those based on the intrinsic mutual authentication of the dynamic signals generated via distinct manipulating manners are expected to present a higher security level. Herein, we demonstrate that low-concentration Yb/Er/Tm-codoped UCNPs with optimized formulation enable modulation of luminous color by manipulating the power of a continuous-wave 980-nm laser or the pulse parameter of the pulsed laser. The crucial role of acting as the red-emission-associated transient energy trapping center that Tm3+ species plays can be attenuated by Yb3+-mediated laser-power-dependent sensitization processes. The key intermediate states associated with red and green emission states are populated and deplete via different processes with distinct rates, which enables dependence of the emission feature on the pulse parameters of the laser. The unique feature of this type of Ln-UCNPs in terms of dual-mode luminous color modulation and its potential are preliminarily verified in our proof-of-concept cross-correlation experiment, which provides a feasible modality for high-level anticounterfeiting applications in specific scenarios such as high-value marketable securities and bond certificates.
研究领域:
材料科学
·
光学
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纳米技术
·
安全与密码学
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产品关联
DOI
高影响因子
Small
·
2018
·Vol. 14
·期 40
·1802292
·
IF 9.598
Keying Zhang,Shuting Song,Shan Huang,Lin Yang,Qianhao Min,Xingcai Wu,Feng Lu,Jun-Jie Zhu
Intracellular microRNAs imaging based on upconversion nanoprobes has great potential in cancer diagnostics and treatments. However, the relatively low detection sensitivity limits their application. Herein, a lock-like DNA (LLD) generated by a hairpin DNA (H1) hybridizing with a bolt DNA (bDNA) sequence is designed, which is used to program upconversion nanoparticles (UCNPs, NaYF 4 @NaYF 4 :Yb, Er@NaYF 4 ) and gold nanoparticles (AuNPs). The upconversion emission is quenched through luminescence resonance energy transfer (LRET). The multiple LLD can be repeatedly opened by one copy of target microRNA under the aid of fuel hairpin DNA strands (H2) to trigger disassembly of AuNPs from the UCNP, resulting in the lighting up of UCNPs with a high detection signal gain. This strategy is verified using microRNA-21 as model. The expression level of microRNA-21 in various cells lines can be sensitively measured in vitro, meanwhile cancer cells and normal cells can be easily and accurately distinguished by intracellular microRNA-21 imaging via the nanoprobes. The detection limit is about 1000 times lower than that of the previously reported upconversion nanoprobes without signal amplification. This is the first time a nonenzymatic signal amplification method has been combined with UCNPs for imaging intracellular microRNAs, which has great potential for cancer diagnosis.
研究领域:
纳米技术
·
分子生物学
·
癌症研究
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2025
·
IF 7.7
Huanhuan Li,Yu Wu,Xorlali Nunekpeku,Yuhan Yang,Jingsa Zhao,Wei Sheng,Yuexia Wang
The increasing presence of Aflatoxin B 1 (AFB 1 ) residues in food continues to raise significant public health concerns. Herein, we designed an upconversion-based sensor integrated into an alginate-polyacrylamide (SA-PAM) hydrogel for on-site AFB 1 detection in food. First, core-shell upconversion nanoparticles (CSUCNPs) with enhanced fluorescence were modified with complementary DNA (cDNA), forming signal probes known as CSUCNPs-cDNA. Simultaneously, NH 2 -ZIF-8@Cu 2+ with a high surface area and specific affinity for aptamer was synthesized using a one-pot method. This was then modified with an AFB 1 -specific aptamer, forming the capture probe Apt-ZIF-8@Cu 2+ . Using base pairing, CSUCNPs-cDNA and Apt-ZIF-8@Cu 2+ were hybridized into sensing probes (CSUCNPs-ZIF-8@Cu 2+ ), which were embedded into SA-PAM hydrogels as stable sensor carrier. Furthermore, the SA-PAM hydrogel showed a reduced swelling ratio of 1.68–1.80 in water and phosphate buffer solution(s) (PBS), which improves stability. In the presence of AFB 1 , the aptamer selectively binds the AFB 1 , increasing electrostatic repulsion between CSUCNPs-cDNA and Apt-ZIF-8@Cu 2+ . This disrupts Förster resonance energy transfer (FRET), resulting in a pronounced “signal-on” fluorescence. This sensor achieves AFB 1 diffusion through the SA-PAM hydrogel, FRET disruption and upconversion signal stabilization within 18 min, faster than traditional methods. Under optimized conditions, the sensor exhibited a clear linear correlation between fluorescence intensity and the logarithm of AFB 1 concentration ranging from 0.1 to 100 µg/kg ( R ² = 0.994), with a detection limit as low as 0.08 µg/kg. Besides, its performance was further validated through tests on spiked wheat, corn, peanut, and water, demonstrating high accuracy and reproducibility with relative standard deviation (RSD) below 3.63 % and recoveries of 85.88–98.04 %.
研究领域:
食品安全
·
分析化学
·
纳米技术
·
生物传感器
·
毒素检测
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产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Yi Xu,Md Mehedi Hassan,Afang Zhu,Huanhuan Li,Quansheng Chen
A new dual-mode optical-nano-sensor was designed to effectively isolate, discriminate and inactivate multiple foodborne pathogens through the combined effort of magnetic SERS tags and functionalized upconversion nanoparticles. The positively charged quaternary ammonium salt (QAS) functionalized upconversion nanoparticles (UCNPs@QAS) were used as a fluorescent probe for the analysis of total bacteria count and inactivation of bacteria, and 4-mercaptophenylboronic acid (4-MPBA) modified magnetic Au@Ag NPs (MAu@Ag@MPBA) were applied as a SERS tag for isolation and classification of bacteria. Various types of bacteria can be captured by UCNPs@QAS through electrostatic interactions and by MAu@Ag@MPBA via the reversible recognition interaction of a boronic acid group of 4-MPBA and peptidoglycan from bacteria simultaneously. And then the formed complex structural assemblies were isolated rapidly from the sample matrix by employing the magnetic force for fast and simple dual-model optical detection. In addition, the novel biosensor was successfully applied to discriminate and detect Escherichia coli , Salmonella , Staphylococcus aureus, and their mixture in contaminated milk with the classification rate of 97.2–100 %. The proposed multi-functional nano-platform was able to sensitively capture, detect, and kill foodborne pathogens in a complex system, which could be deployed successfully towards the monitoring of food safety.
研究领域:
生物传感
·
食品安全
·
纳米技术
·
微生物学
✓ 已验证
产品关联
DOI
高影响因子
Nanoscale
·
2014
·Vol. 6
·期 21
·13242-13252
·
IF 6.739
Lining Sun,Xiaoqian Ge,Jinliang Liu,Yannan Qiu,Zuwu Wei,Bo Tian,Liyi Shi
A series of new multifunctional nanomesoporous materials based on upconversion nanophosphors NaYF4:Yb,Tm@NaGdF4 (UCNPs) and lanthanide complexes were designed and synthesized through mesoporous capping UCNPs nanophosphors and linking lanthanide (Ln) complexes. The obtained UCNPs@mSiO2-Ln(dbm)4 (Ln = Eu, Sm, Er, Nd, Yb) materials can achieve downconversion and upconversion luminescence to show multicolor emission (covering the spectral region from 450 nm to 1700 nm) under visible-light excitation and 980 nm excitation, respectively. In addition, low cytotoxicity and good biocompatibility was found as determined by methyl thiazolyl tetrazolium assay, and the nanomesoporous materials were successfully applied to cell imaging in vitro based on Eu3+ luminescence (under 405 nm excitation) and small animal imaging based on Tm3+ luminescence (under 980 nm excitation). The doped Gd3+ ion endows the nanomesoporous materials UCNPs@mSiO2-Ln(dbm)4 with effective T1 signal enhancement, which affords them as potential magnetic resonance imaging (MRI) contrast agents. Therefore, our results may provide more exciting opportunities for multimodal bioimaging and multifunctional applications.
研究领域:
材料科学
·
生物医学工程
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Theranostics
·
2016
·
IF 8.854
Caixia Yue,Chunlei Zhang,Gabriel Alfranca,Yao Yang,Xinquan Jiang,Yuming Yang,Fei Pan,Jesús M. de la Fuente,Daxiang Cui
Many drug controlled release methods have been integrated in multifunctional nanoparticles, such as pH-, redox-, temperature-, enzyme-, and light-responsive release. However, few report is associated with the ROS responsive drug controlled release. Herein, a thioketal linker-based ROS responsive drug (camptothecin conjugated with thioketal linker, abbreviated as TL-CPT) was prepared and the thioketal linker could be cleaved by ROS(reactive oxygen species). To achieve cancer simultaneous optical imaging, photodynamic therapy and chemotherapy, the photosensitizer Chlorin e6(Ce6), TL-CPT and carboxyl-mPEG were loaded on the upconversion nanoparticles (UCNPs), which were named as Ce6-CPT-UCNPs. Under 980 nm laser irradiation, Ce6-CPT-UCNPs emitted a narrow emission band at 645-675 nm which was overlapped with Ce6 absorption peak. Ce6 absorbed the light to produce ROS, which was used for photodynamic therapy and to cleave the thioketal linker in Ce6-CPT-UCNPs to release camptothecin for chemotherapy. Meanwhile, Ce6 absorbed the light, was used for near-infrared fluorescence imaging. The in vivo biodistribution studies showed that the prepared nanoparticles had high orthotopic lung cancer targeting efficiency. The in vivo therapeutic results demonstrated that NCI-H460 lung cancers could be completely eliminated by combining chemo- and photodynamic therapy under 980 nm laser irradiation. The prepared multifunctional Ce6-CPT-UCNPs have great potential in applications such as cancer targeted fluorescent imaging, simultaneous ROS activated chemo- and photodynamic therapy in near future.
研究领域:
生物医学工程
·
肿瘤学
·
药剂学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2022
·
IF 9.221
Xiaodan Ding,Waqas Ahmad,Muhammad Zareef,Yawen Rong,Yunlian Zhang,Jizhong Wu,Qin Ouyang,Quansheng Chen
In this study, an ultra-sensitive and selective biosensor for the detection of enrofloxacin (ENR) in complex matrices has been established. The study intelligently converges the spectral properties of core-shell upconverted nanoparticles (UCNPs) and the excellent adsorption satiating aptitudes of metal organic frameworks (MOFs). The principle was based on the fluorescence resonance energy transfer (FRET) between aptamer-modified UCNPs as the donors and MOFs MIL-101(Cr) as the effective receptors. The spectral overlap between UCNPs fluorescence emission and MIL-101(Cr) absorption generated the FRET process under the action of π-π stacking interactions. The presence of ENR induced conformational changes in the aptamer via preferential binding, resulting in a decline in the nucleobase exposure on the surface of MIL-101(Cr) and eventually in the fluorescence recovery. The fluorescence intensity (FL) was enhanced as the ENR concentration was increased. The detection limit was 0.034 ng⋅mL −1 and the linear calibration curve between logarithmic ENR concentration and FL was achieved in the range of 0.1–1000 ng⋅mL −1 . The applicability of the platform for ENR recognition was confirmed in real fish and shrimp samples and further validated by a standard HPLC technique with no significant difference (p > 0.05). The current UCNPs-MIL-101(Cr) aptasensor proposes a fast, sensitive and quantitative detection approach for ENR in aquatic products.
研究领域:
分析化学
·
环境科学
·
食品科学
·
纳米技术
·
生物传感器
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）