IUBMB LIFE
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2024
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IF 3.7
Yexuan Lin,Na Sun,Dengyuan Liu,Xinmeng Yang,Yixin Dong,Chengyu Jiang
In 2020, the number of deaths caused by lung cancer worldwide reached 1,796,144, making it the leading cause of cancer-related deaths. Cyclooxygenase-2/prostaglandin endoperoxide synthase 2 ( COX-2/PTGS2 ) is overexpressed in lung cancer, which promotes tumor proliferation, invasion, angiogenesis, and resistance to apoptosis. Here, we report that the oligonucleotide drug HQi-sRNA-2 from Traditional Chinese Medicine Huangqin targeting COX-2/PTGS2 significantly inhibited proliferation, migration, and invasion and induced apoptosis in the human lung cancer cell line NCI-H460. Oral delivery of HQi-sRNA-2 bencaosomes prolonged survival, reduced tumor burden, and maintained weight in a spontaneous mouse lung cancer model. Compared with paclitaxel, HQi-sRNA-2 may be less toxic and have approximately equal efficacy in reducing tumor burden. Our previous studies reported that herbal small RNAs (sRNAs) are functional medical components. Our data suggest that sphingosine (d18:1)-HQi-sRNA-2 bencaosomes, targeting COX-2/PTGS2 and downregulating the PI3K and AKT signaling pathways, may provide novel therapeutics for lung cancer.
研究领域:
肿瘤学
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药理学
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分子生物学
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中医药
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Heliyon
·
2024
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IF 3.4
Doudou Lu,Ling Yuan,Xiaoyan Ma,Fandi Meng,Duojie Xu,Shumin Jia,Zhaozhao Wang,Yahong Li,Zhe Zhang,Yi Nan
Background Polyphyllin is a class of saponins extracted from Paris polyphylla rhizomes and has been used in clinical application in China for more than 2000 years. However, the mechanism for treating gastric cancer (GC) is still unclear. This study was designed to predict the targets and mechanisms of total Polyphyllin from Paris polyphylla rhizomes for the treatment of GC. Method Firstly, PubChem and Swiss Target Prediction databases were utilized to collect the 12 ingredients of total Polyphyllin from Paris polyphylla rhizomes and their targets. GC-related genes were obtained from the GEO database. Then the intersecting targets to all these molecules that identified using Venny. Secondly, the intersecting targets were imported into STRING platform for protein-protein interaction (PPI) network. Moreover, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were conducted in DAVID website. In addition, the GEPIA was applied to perform the expression levels, transcript levels, staging, and overall survival of hub genes. In addition, we used AutoDock Vina to evaluate binding affinity of molecular docking between key ingredients and anti-GC targets. In vitro cell experiments, we detected the cell viability of gastric cancer cells at 24, 36, and 48 h using CCK-8 assay. The G0/G1 of cell cycle and apoptosis were detected by flow cytometry. Finally, quantitative real time polymerase chain reaction (qRT-PCR) was used to detect the level of hub genes, and Western blot was used to detect the changes of PI3K/Akt signal pathway. Results Firstly, we identified 12 ingredients and 286 targets of total Polyphyllin. A total of 2653 GC-related differentially expressed genes (DEGs) were collected, including 1366 up-regulated genes and 1287 down-regulated genes. Moreover, 45 targets were obtained after intersection. Secondly, results of the GO enrichment suggested that these genes were closely related to cell proliferation, migration and aging. KEGG analysis suggested that Polyphyllin in GC therapy were mostly regulated by multiple pathways, including the pathways in cancer, calcium signaling pathway, Rap1 signaling pathway, phospholipase D signaling pathway, etc. In addition, GEPIA results exhibited that PDGFRB, KIT, FGF1, GLI1, F2R, and HIF1A were associated with GC progression, stage, and survival. Besides, the molecular docking results further confirmed that the binding energy of Polyphyllin Ⅲ with HIF1A was minimal. In vitro cell experiments, Polyphyllin Ⅲ inhibited the cell viability of gastric cancer cells, blocked the cell cycle G0/G1 phase, and induced cell apoptosis. In addition, Polyphyllin Ⅲ down-regulated the mRNA levels of PDGFRB, KIT, FGF1, GLI1, F2R , and HIF1A , and regulated the PI3K/Akt signal pathway. Conclusions The results revealed that total Polyphyllin treated GC through multiple targets, multiple channels, and multiple pathways. In addition, Polyphyllin Ⅲ played an anti-gastric cancer role by inhibiting the proliferation of gastric cancer.
研究领域:
肿瘤学
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药理学
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生物信息学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2024
·
IF 9.4
Hao Wang,Xueya Deng,Alina Bari,Mingzheng Gu,Man Lin,An Gao,Xiaomin Huang,Xiaojun Zhang
In order to enhance the energy efficiency of water electrolysis, it is imperative to devise electrocatalysts for oxygen evolution reaction that are both non-precious metal-based and highly efficient. Efficient catalyst design is generally based on electronic structural engineering. Considering the electronegativity disparity between selenium (Se) and tellurium (Te), the tunable bandgaps, and the conductive metallic nature of Te . We designed a material wherein Te atoms are uniformly doped onto the surface of Cobalt tetra selenide (Co 3 Se 4 ) nanorods, leading to the synthesis of a defect-rich material. Experimental results demonstrate that Te doping in Co 3 Se 4 increases active sites and optimizes the electronic structure of Co cations, enhancing the design of multi-defect structures. This promotes the generation of the Co(oxy) hydroxide (CoOOH) active phase, enhancing catalytic activity by maximizing the binding strength between Co sites and oxygenated intermediates. Te-Co 3 Se 4 nanorods exhibit good catalytic activity for oxygen evolution reactions, with an overpotential of 269 mV at a driving current density of 50 mA cm −2 and excellent stability in alkaline media (over 100 h). This discovery indicates the feasibility of strategically combining various imperfect structures, thereby unlocking the latent potential of diverse catalysts in electrocatalytic reactions.
研究领域:
材料科学
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电化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2024
·
IF 5.7
Ying Liang,Zi Wang,Deyang Huo,Jun-nan Hu,Lingjie Song,Xiaochi Ma,Shuang Jiang,Wei Li
In recent years, there has been a growing concern regarding health issues arising from exposure to nanoplastics (Nps) in the natural environment. The Nps bioaccumulate within the body via the circulatory system and accumulate in the liver, resulting in damage. Previous studies have demonstrated that maltol, derived from red ginseng (Panax ginseng C.A. Meyer) as a Maillard product, exhibits hepatoprotective effects by alleviating liver damage caused by carbon tetrachloride or cisplatin. In order to explore the specific mechanism of maltol in improving hepatotoxicity induced by Nps, mice exposed to 100 mg/kg Nps were given maltol at doses of 50 and 100 mg/kg, respectively. The results showed that Nps induced an increase in the levels of liver apoptotic factors BAX and cytochrome c, a decrease in the levels of the autophagy key gene LC3 II/I, and an increase in P62. It also caused oxidative stress by affecting the Nrf2/HO-1 pathway, and a decrease in GPX4 protein expression suggested the occurrence of ferroptosis. However, treatment with maltol significantly improved these changes. In addition, maltol (2, 4, and 8 μM) also protected human normal liver L02 cells from Np (400 μg/mL)-induced damage. Our data suggest that maltol could ameliorate Np-induced L02 cytotoxicity by reducing autophagy-dependent oxidative stress, exhibiting similar protective effects in vitro as in vivo. This study helps shed light on the specific molecular mechanism of Np-induced hepatotoxicity. For the first time, we studied the protective effect of maltol on Np-induced liver injury from multiple perspectives, expanding the possibility of treatment for diseases caused by environmental pollutants.
研究领域:
毒理学
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药理学
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环境科学
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细胞生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2025
·
IF 9.7
Yu Zhang,Rong Li,Qi Guo,Fangxiang Song,Kang Kai,Qianlin Chen
The emergence of the lithium-ion battery as a subject of intense research interest has propelled of high-energy–density LiFe x Mn 1-x PO 4 (LFMP) becoming a prominent area of investigation. However, the material suffers from inherently low electronic conductivity due to its olivine structure, which imposes severe constraints on electron transport kinetics, thus adversely impacting both charge–discharge rates and overall electrochemical performance. We propose an innovative protocol for high-precision reaction mechanism modulation. By employing Fe 3 (PO 4 ) 2 ·8H 2 O with strategically enhanced (020) crystal plane exposure as a pivotal precursor, we synthesized LiFe 0.75 Mn 0.25 PO 4 /C cathode material featuring a shorter ion diffusion path. Comprehensive characterization coupled with electrochemical validation revealed that the resultant cathode material exhibits a smaller particle size and more uniform morphology, along with a superior rate performance and cycle stability. The discharge specific capacity is 144.1 mAh g −1 and the capacity retention reaches 96.1 % over 1000 cycles at a 1C rate. The findings demonstrate that the regulation of the growth trajectory of the precursor Fe 3 (PO 4 ) 2 ·8H 2 O crystal plane can markedly enhance the electronic conductivity and Li + mobility of the cathode material, thereby optimising the electrochemical performance.
研究领域:
材料科学
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电化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
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2025
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IF 13.2
Tianze Wang,Hui Chi,Danying Zhao,Qitong Wang,Zhenhua Jiang,Yunhe Zhang
In recent years, boron nitride nanosheets (BNNS) have been widely used in the development of polymer-based thermal interface materials (TIM) due to their excellent thermal conductivity and electrical insulation. However, the thermal performance of these TIMs is still unsatisfactory, mainly because BNNS do not connect effectively in the polymer matrix. The lamellar structure of a crepe cake in life provides inspiration for the design of this study. In this study, TIM with long-range ordered structure was prepared by using the freezing process guided by double temperature gradient. The long-range ordered structure forces BNNS to be tightly connected and highly oriented between polymer layers, thus providing long-range conduction paths for phonons. The composite prepared by this strategy exhibits excellent in-plane thermal conductivity (10.4 W/(m·K)), volume resistivity, tensile strength and thermal stability at only 20 vol% BNNS loading, which makes it has a broad application prospect in the field of electronic device packaging. In addition, the strategy provided by the study can not only be applied to the development of thermal conductivity composites, but also can be extended to different material combinations of various functional composites.
研究领域:
材料科学
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热管理技术
✓ 已验证
产品关联
DOI
高影响因子
TOXICOLOGY
·
2024
·
IF 4.8
Di Ouyang,Yiren Xiong,Zuqing Hu,Jiayi He,Shanshan He,Renyi Liu,Zhenjie Gao,Dalin Hu
The mechanistic target of rapamycin (RAPA) complex 1 (mTORC1) - transcription factor EB (TFEB) pathway plays a crucial role in response to nutritional status, energy and environmental stress for maintaining cellular homeostasis. But there is few reports on its role in the toxic effects of arsenic exposure and the related mechanisms. Here, we show that the exposure of bronchial epithelial cells (BEAS-2B) to sodium arsenite promoted the activation of mTORC1 (p-mTORC1) and the inactivation of TFEB (p-TFEB), the number and activity of lysosomes decreased, the content of reduced glutathione (GSH) and superoxide dismutase (SOD) decreased, the content of malondialdehyde (MDA) increased, the DNA and chromosome damage elevated. Further, when mTORC1 was inhibited with RAPA, p-mTORC1 and p-TFEB down-regulated, GSH and SOD increased, MDA decreased, the DNA and chromosome damage reduced significantly, as compared with the control group. Our data revealed for the first time that mTORC1 - TFEB pathway was involved in sodium arsenite induced lysosomal alteration, oxidative stress and genetic damage in BEAS-2B cells, and it may be a potential intervention target for the toxic effects of arsenic.
研究领域:
毒理学
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细胞生物学
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分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2020
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IF 7.489
Jiaxin Cui,Tao Lu,Fanghua Li,Yulin Wang,Jiandu Lei,Wenjing Ma,Yan Zou,Chaobo Huang
Air particulate pollution from ever-increasing industrialization poses an enormous threat to public health. Thus, the development of a green air filter with high efficiency and performance is of urgent necessity. In this study, we introduce a new effective air filtration membrane that can be used for outdoor protection. The air filter’s composite nanofibre materials were prepared from polyvinyl alcohol (PVA)-sodium lignosulfonate (LS) via a “green” electrospinning method and thermal crosslinking. The addition of LS helped increase the PM 2.5 removal efficiency compared to that of a pure PVA nanofibre membrane. The pressure drops of the electrospun PVA-LS membranes exceeded those of the pristine PVA air filter. The remarkable air filtration performance was maintained even after 10 cycles of circulation filtration. In addition, the PVA-LS composite nanofibre membrane exhibited excellent mechanical properties and transparency due to the introduction of LS. This study provides new insight into the design and development of high-performance and high-visibility green filter media, which include personal protection and building screens.
研究领域:
环境科学
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材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMOSPHERE
·
2023
·
IF 8.8
Jiayi Luo,Yunqiang Yi,Zhanqiang Fang
In order to fully exploit the potential of magnetic biochar-based persulfate (PS) systems, N was utilized to modify the magnetic biochar-based catalysts through impregnation-pyrolysis method. A typical antifungal drug, metronidazole (MNZ), is selected as the target pollutant to score the reactivity of as-synthetic nitrogen-rich magnetic biochar (NMBC) catalysts. In the modified system, 99.6% of MNZ was removed, 13.6 times of that in the unmodified system. Active radical verification experiments showed that 1O2 was the key active radical. Various characterization showed that the nitrogen-rich significantly improved the persistent free radical, defect degree, content of oxygen-containing groups, electrochemical conductivity and other catalytic activity related properties. Physicochemical characterization, Fe(II) semi-quantitative analysis and masking experiments confirmed that the doping of magnetic biochar with nitrogen increased its Fe(II) content (23.79 mg/g), approximately 2.6 times higher than that of pristine magnetic biochar. Moreover, N induces strong electron accretion of Fe atom through coordination bond, which leads to the increase of electron density on the Fe atom, which increases the content of Fe (II) in the material, thus improving the ability of the material to activate PS to generate 1 O 2 , and promoting the degradation reaction of MNZ. This paper provides a method to improve the activation performance of magnetic biochar.
研究领域:
环境科学
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材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Environmental Chemical Engineering
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2024
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IF 7.4
Cong Pan,Yunqiao Wang,Chenyang Fu,Jirong Lan
Smelt slag-based environmental catalytic materials offer a sus(tainable and valuable approach to solid waste management. In this study, a novel peroxymonosulfate (PMS) catalyst (MCT-2) was prepared from copper slag (MCT-1) by a non-calcined method. Subsequently, the MCT/PMS-AOP catalytic system was established. The copper slag, mainly composed of Si, Ca, Mg, Fe and O, was converted into MCT-2 by hydrothermal treatment, NaOH etching and edta-2na-induced crystallization, and finally, the molecular self-loading of MCT was achieved by the "one-pot method" under hydrothermal conditions. MCT-2 exhibits a layered surface morphology and CaFeO 3 phase. In the MCT-2/PMS system, the dominant reactive oxygen species (ROSs) generated were SO 4 •− and HO • . The performance of the system was evaluated using CBZ as a simulated pollutant and actual river water as a carrier, and it was found that MCT-2/PMS degraded CBZ, BPA, DCF and ATZ up to 99 %. Further mechanistic studies showed that MCT-2 has relatively high catalytic activity and the lowest reaction energy barrier compared with similar materials. The easy availability of raw materials, environmentally friendly preparation method, and high degradation efficiency make MCT/PMS-AOP have a broad application prospect.
研究领域:
环境科学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF CATALYSIS
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2025
·
IF 6.5
Shan Wu,Jian Shi,Qian Duan,Ludan Shang,Xiaodong Zhang,Yuhan Duan
Porphyrins and phthalocyanines are considered as visible light catalysts with great development potential due to their unique π-conjugated structure, strong visible light absorption and structural tunability. However, they are easy to aggregate and difficult to dissolve, and their application in the field of photocatalysis is limited. Based on the above problems, in this study, three four-arms star-shaped thermosensitive copolymers (PcZn-(PNIPAM n -co-PUMPP m ) 4 ) photocatalysts with zinc phthalocyanine as the core and porphyrin thermosensitive copolymer as the arms was constructed by using RAFT polymerization technology and the ’core first’ route. The visible light catalytic activity of the prepared star-shaped copolymers was evaluated by using 1,5-dihydroxynaphthalene (DHN) and methylene blue (MB) as model pollutants. The three copolymers were found to be effective in oxidizing DHN to juglone and degrading MB. Among them, PcZn-(PNIPAM 33.5 -co-PUMPP 1.6 ) 4 (P3) showed the highest DHN conversion efficiency (100.0 %), reaction yield (70.0 %) and MB degradation (96.9 %). 1 O 2 and O 2 · - are the main active substances in the visible photocatalytic process. Based on UV–vis absorption spectra, Fluorescence spectra, EIS Nyquist plots and transient photocurrents, it is confirmed that P3 has a wider range of visible light absorption, and there is effective energy and electron transfer between TTPcZn and UMPP units in P3, which makes it have higher catalytic activity. More importantly, the lower critical solution temperature (32.6 ∼ 32.9 °C) of P3 makes it possible to achieve homogeneous catalysis and heterogeneous recovery through simple temperature regulation in the catalytic process, which makes P3 have good reusability. This work paves a way for the construction of high-performance and recyclable visible polymer photocatalyst.
研究领域:
材料科学
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环境科学
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化学工程
✓ 已验证
产品关联
DOI
高影响因子
FOOD AND CHEMICAL TOXICOLOGY
·
2024
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IF 3.9
Yiren Xiong,Jiayi He,Shanshan He,Zuqing Hu,Di Ouyang,Renyi Liu,Zhenjie Gao,Weiguang Zhang,Zhujun Kang,Shuyi Lan,Yang Wang,Fatoumata Diallo,Dalin Hu
Lead is a ubiquitous environmental chemical with various toxic damage to human body. This investigation aimed to explore the intervention effect of human umbilical cord mesenchymal stem cells derived exosomes (HUC-MSC-exo) on the neurotoxicity of lead and the relevant mechanism. Differential gradient ultracentrifugation was adopted to isolate HUC-MSC-exo. Nanoparticle tracking assay (NTA), Transmission electron microscope (TEM) technology and exosomal specific biomarkers CD9, CD63 and CD81 were adopted for exosomal characterization. Human neuroblastoma cell (SH-SY5Y) was used as the recipient cell. Confocal laser scanning microscope analysis was conducted to confirm the intake of HUC-MSC-exo by SH-SY5Y cells. Cell migration ability, apoptosis, IL-6, IL-1β and TNF-α were analyzed. The role of miR-26a-5p/PTEN axis was assessed. The result showed that the exposure of SH-SY5Y cells to lead activated the miR-26a-5p/PTEN pathway by down-regulating miR-26a-5p and up-regulating PTEN expression, which was related to the significantly decreased cell migration and increased apoptosis, as well as significantly enhanced levels of inflammatory cytokine as compared with the control. While HUC-MSC-exo could significantly alleviate the cytotoxicity, apoptosis and inflammatory effects induced by lead on SH-SY5Y cells via partially restoring miR-26a-5p/PTEN pathway. Herein, we conclude that HUC-MSC-exo can alleviate lead-induced toxic effects on SH-SY5Y cells partially through miR-26a-5p/PTEN pathway.
研究领域:
毒理学
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干细胞研究
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神经科学
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分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）